pycom12g18410

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0001553
Physical Location & Seq
Forward (+)
20563123 .. 20566304
3182 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1191 bp
ATGAAGCTTTTCTGGGTTAGCATTGTGATTTCACTTGCTTTCAGCAAATGGGCTGCTGGGTTTATGGAGTACGGCTTCAATGAAACAGAGATGGCTTTGTTGGAGGGATATGGAGAATCAAAGGTTGGAGCTAACTTGCTGATGGTAGGACTGACCCTCATTGGAGGAGCTGGGGCCAAAGGAGCAGTCTGCTTGGATGGAACCTTACCGGGGTATCATTTGCATCGCGGATACGGGTCAGGAGCAAATAGTTGGCTCATTCAATTGGAGGGGGGAGGATGGTGTAACAACATTAGAACTTGTGTTTATCGAAAAACAACAAGGCGGGGATCATCAAAATACATGGAAAAACAGTTGGTATTCAGTGGAATACTGAGCAATAAAGCTGAAGAAAATCCTGATTTTTTCAACTGGAACAGAGTCAAGGTTCGTTATTGCGATGGTGCTTCTTTTTCTGGGGATAGTGAAGATAAGGCCAAGGAACTGCAATTTAGAGGAGAGCGTATATGGTTGGCTGCAATGGAAGACTTGAAGTCAAAGGGCATGAGCAACGCCAAGCAGGCTCTTCTTTCGGGTTGCTCTGCCGGTGGTGTTGCAGCTATTTTGCACTGTGATAAGTTCCGGGGTTTAATGCCAGGAACTACGAAAGTGAAGTGCCTGAGTGATGCTGGATTGTTTCTTGATGCGAATGACGTTTCTGGTGGACGCACACTCAGGAATTTATATGGTGGCATTGTCGGCTTGCAGTGCTTCTTCCCTCAGAACTTGATCGATCCAGCATCAAAACCCCACTGTTTATACTCAATGCAGCATATGATTCGTGGCAGCCAGACCAGTTTAGCTCCACCGGCAGCAGATCGTGCCGGCTACTGGCGCGATTGCAGATTAAACCATGAAAAATGTACTCCATCACAGATCAATTTTCTTCAAGGATTCAGGAATCAAATGCTGAATGCAGTAAAACAGTTCTCTATGTCTGAACAAAACGGGTTGTTTATAAATTCGTGTTTTGCGCACTGCCAATCCGAGAGGCAGGATACATGGTTTGCTGATGATTCTCCAGTTATTGGAAAGAAGGCAATTGCTATAGCAGTTGGAGATTGGTATTTCGACCGAACAAGCGTGAAGATCATAGATTGTCCCTACCCATGTGACAATTCTTGCCACAATCTGAATTTCGAAAAAGCTTAG

Protein Analysis

397

Amino Acids

43.7

Weight (kDa)

8.51

Isoelectric Point (pI)

34.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 46 - 389 4.2e-142 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 998
Acc16I TGCGCA 1 cut(s) 1014
AccB7I CCANNNNNTGG 1 cut(s) 1067
AccII CGCG 2 cut(s) 228, 876
AciI CCGC 2 cut(s) 228, 325
AclWI GGATC 2 cut(s) 337, 767
AcsI RAATTY 3 cut(s) 718, 1000, 1174
AcuI CTGAAG 1 cut(s) 408
AfaI GTAC 2 cut(s) 71, 904
AfiI CCNNNNNNNGG 3 cut(s) 210, 870, 1067
AgsI TTSAA 5 cut(s) 79, 263, 409, 532, 929
AhdI GACNNNNNGTC 1 cut(s) 532
AjnI CCWGG 1 cut(s) 634
AjuI GAANNNNNNNTTGG 2 cut(s) 108, 140
AluBI AGCT 7 cut(s) 7, 131, 170, 386, 599, 842, 1187
AluI AGCT 7 cut(s) 7, 131, 170, 386, 599, 842, 1187
AlwI GGATC 2 cut(s) 337, 767
AoxI GGCC 2 cut(s) 174, 474
ApeKI GCWGC 6 cut(s) 53, 515, 596, 808, 825, 851
ApoI RAATTY 3 cut(s) 718, 1000, 1174
ArsI GACNNNNNNTTYG 2 cut(s) 171, 203
Asp700I GAANNNNTTC 1 cut(s) 8
AspLEI GCGC 2 cut(s) 876, 1015
AspS9I GGNCC 1 cut(s) 174
AsuC2I CCSGG 2 cut(s) 210, 623
AsuII TTCGAA 1 cut(s) 1179
BaeI ACNNNNGTAYC 2 cut(s) 197, 230
BbsI GAAGAC 1 cut(s) 531
BbvI GCAGC 6 cut(s) 40, 502, 608, 820, 837, 863
BccI CCATC 6 cut(s) 85, 136, 191, 273, 434, 916
BceAI ACGGC 1 cut(s) 88
BcgI CGANNNNNNTGC 2 cut(s) 800, 834
BciT130I CCWGG 1 cut(s) 636
BciVI GTATCC 2 cut(s) 224, 1030
BcnI CCSGG 2 cut(s) 210, 623
BfmI CTRYAG 1 cut(s) 1086
BfuI GTATCC 2 cut(s) 224, 1030
BglI GCCNNNNNGGC 1 cut(s) 560
BisI GCNGC 6 cut(s) 54, 516, 597, 809, 826, 852
BlsI GCNGC 6 cut(s) 55, 517, 598, 810, 827, 853
Bme1390I CCNGG 3 cut(s) 210, 623, 636
BmeRI GACNNNNNGTC 1 cut(s) 532
BmgT120I GGNCC 1 cut(s) 174
BmiI GGNNCC 2 cut(s) 175, 202
BmrFI CCNGG 3 cut(s) 210, 623, 636
BmsI GCATC 4 cut(s) 232, 655, 673, 788
BpiI GAAGAC 1 cut(s) 531
BpmI CTGGAG 1 cut(s) 1044
Bpu14I TTCGAA 1 cut(s) 1179
BpuMI CCSGG 2 cut(s) 210, 623
Bsa29I ATCGAT 1 cut(s) 771
BsaJI CCNNGG 3 cut(s) 209, 477, 622
Bsc4I CCNNNNNNNGG 3 cut(s) 210, 870, 1067
Bse118I RCCGGY 3 cut(s) 584, 847, 863
Bse1I ACTGG 4 cut(s) 416, 834, 875, 1061
Bse3DI GCAATG 1 cut(s) 525
BseBI CCWGG 1 cut(s) 636
BseCI ATCGAT 1 cut(s) 771
BseDI CCNNGG 3 cut(s) 209, 477, 622
BseGI GGATG 2 cut(s) 202, 284
BseLI CCNNNNNNNGG 3 cut(s) 210, 870, 1067
BseMI GCAATG 1 cut(s) 525
BseMII CTCAG 4 cut(s) 365, 650, 727, 773
BseNI ACTGG 4 cut(s) 416, 834, 875, 1061
BseRI GAGGAG 2 cut(s) 180, 510
BseXI GCAGC 6 cut(s) 40, 502, 608, 820, 837, 863
BseYI CCCAGC 2 cut(s) 56, 170
Bsh1236I CGCG 2 cut(s) 228, 876
Bsh1285I CGRYCG 1 cut(s) 1114
BshFI GGCC 2 cut(s) 176, 476
BshVI ATCGAT 1 cut(s) 771
BsiEI CGRYCG 1 cut(s) 1114
BsiSI CCGG 5 cut(s) 209, 585, 622, 848, 864
BslFI GGGAC 1 cut(s) 1125
BslI CCNNNNNNNGG 3 cut(s) 210, 870, 1067
BsmFI GGGAC 1 cut(s) 1125
BsmI GAATGC 1 cut(s) 958
BsnI GGCC 2 cut(s) 176, 476
Bsp119I TTCGAA 1 cut(s) 1179
Bsp143I GATC 6 cut(s) 329, 768, 772, 856, 915, 1128
BspACI CCGC 2 cut(s) 228, 325
BspANI GGCC 2 cut(s) 176, 476
BspCNI CTCAG 4 cut(s) 366, 651, 726, 772
BspDI ATCGAT 1 cut(s) 771
BspFNI CGCG 2 cut(s) 228, 876
BspLI GGNNCC 2 cut(s) 175, 202
BspPI GGATC 2 cut(s) 337, 767
BspQI GCTCTTC 1 cut(s) 570
BspT104I TTCGAA 1 cut(s) 1179
BsrDI GCAATG 1 cut(s) 525
BsrFI RCCGGY 3 cut(s) 584, 847, 863
BsrI ACTGG 4 cut(s) 416, 834, 875, 1061
BssAI RCCGGY 3 cut(s) 584, 847, 863
BssECI CCNNGG 3 cut(s) 209, 477, 622
BssMI GATC 6 cut(s) 329, 768, 772, 856, 915, 1128
BssT1I CCWWGG 1 cut(s) 477
Bst2UI CCWGG 1 cut(s) 636
Bst4CI ACNGT 4 cut(s) 354, 611, 794, 966
Bst6I CTCTTC 1 cut(s) 570
BstAPI GCANNNNNTGC 1 cut(s) 860
BstBI TTCGAA 1 cut(s) 1179
BstC8I GCNNGC 3 cut(s) 561, 743, 865
BstDEI CTNAG 5 cut(s) 374, 659, 713, 759, 1188
BstF5I GGATG 2 cut(s) 202, 284
BstFNI CGCG 2 cut(s) 228, 876
BstHHI GCGC 2 cut(s) 876, 1015
BstKTI GATC 6 cut(s) 332, 771, 775, 859, 918, 1131
BstMBI GATC 6 cut(s) 329, 768, 772, 856, 915, 1128
BstMCI CGRYCG 1 cut(s) 1114
BstMWI GCNNNNNNNGC 7 cut(s) 182, 560, 738, 747, 848, 860, 873
BstNI CCWGG 1 cut(s) 636
BstSCI CCNGG 3 cut(s) 208, 621, 634
BstSFI CTRYAG 1 cut(s) 1086
BstUI CGCG 2 cut(s) 228, 876
BstV1I GCAGC 6 cut(s) 40, 502, 608, 820, 837, 863
BstV2I GAAGAC 1 cut(s) 531
Bsu15I ATCGAT 1 cut(s) 771
BsuI GTATCC 2 cut(s) 224, 1030
BsuRI GGCC 2 cut(s) 176, 476
BsuTUI ATCGAT 1 cut(s) 771
BtgZI GCGATG 2 cut(s) 209, 453
BtsCI GGATG 2 cut(s) 202, 284
BtsI GCAGTG 2 cut(s) 752, 1015
BtsIMutI CAGTG 5 cut(s) 370, 607, 752, 790, 1015
Cac8I GCNNGC 3 cut(s) 561, 743, 865
CfoI GCGC 2 cut(s) 876, 1015
Cfr10I RCCGGY 3 cut(s) 584, 847, 863
Cfr13I GGNCC 1 cut(s) 174
ClaI ATCGAT 1 cut(s) 771
CseI GACGC 1 cut(s) 714
Csp6I GTAC 2 cut(s) 70, 903
CviAII CATG 5 cut(s) 343, 544, 893, 1041, 1149
CviQI GTAC 2 cut(s) 70, 903
DdeI CTNAG 5 cut(s) 374, 659, 713, 759, 1188
DpnI GATC 6 cut(s) 331, 770, 774, 858, 917, 1130
DpnII GATC 6 cut(s) 329, 768, 772, 856, 915, 1128
DriI GACNNNNNGTC 1 cut(s) 532
Eam1104I CTCTTC 1 cut(s) 570
Eam1105I GACNNNNNGTC 1 cut(s) 532
EarI CTCTTC 1 cut(s) 570
Eco130I CCWWGG 1 cut(s) 477
Eco57I CTGAAG 1 cut(s) 408
EcoRII CCWGG 1 cut(s) 634
EcoT14I CCWWGG 1 cut(s) 477
ErhI CCWWGG 1 cut(s) 477
FaeI CATG 5 cut(s) 346, 547, 896, 1044, 1152
FaqI GGGAC 1 cut(s) 1125
FatI CATG 5 cut(s) 342, 543, 892, 1040, 1148
FauI CCCGC 1 cut(s) 318
FauNDI CATATG 1 cut(s) 813
Fnu4HI GCNGC 6 cut(s) 54, 516, 597, 809, 826, 852
FokI GGATG 2 cut(s) 209, 291
Fsp4HI GCNGC 6 cut(s) 54, 516, 597, 809, 826, 852
FspI TGCGCA 1 cut(s) 1014
GlaI GCGC 2 cut(s) 875, 1014
GluI GCNGC 6 cut(s) 54, 516, 597, 809, 826, 852
GsaI CCCAGC 2 cut(s) 60, 174
GsuI CTGGAG 1 cut(s) 1044
HaeIII GGCC 2 cut(s) 176, 476
HapII CCGG 5 cut(s) 209, 585, 622, 848, 864
HgaI GACGC 1 cut(s) 714
HhaI GCGC 2 cut(s) 876, 1015
Hin1II CATG 5 cut(s) 346, 547, 896, 1044, 1152
Hin6I GCGC 2 cut(s) 874, 1013
HinP1I GCGC 2 cut(s) 874, 1013
HindIII AAGCTT 2 cut(s) 5, 1185
HinfI GANTC 6 cut(s) 116, 420, 817, 933, 940, 1055
HpaII CCGG 5 cut(s) 209, 585, 622, 848, 864
Hpy166II GTNNAC 1 cut(s) 704
Hpy188I TCNGA 4 cut(s) 762, 979, 1027, 1173
Hpy188III TCNNGA 5 cut(s) 240, 398, 680, 715, 937
Hpy8I GTNNAC 1 cut(s) 704
HpyAV CCTTC 1 cut(s) 1069
HpyCH4III ACNGT 4 cut(s) 354, 611, 794, 966
HpyCH4IV ACGT 1 cut(s) 693
HpyCH4V TGCA 9 cut(s) 223, 487, 518, 596, 607, 745, 808, 882, 956
HpyF10VI GCNNNNNNNGC 7 cut(s) 182, 560, 738, 747, 848, 860, 873
HpyF3I CTNAG 5 cut(s) 374, 659, 713, 759, 1188
HpySE526I ACGT 1 cut(s) 693
Hsp92II CATG 5 cut(s) 346, 547, 896, 1044, 1152
HspAI GCGC 2 cut(s) 874, 1013
KroI GCCGGC 1 cut(s) 863
KroNI GCCGGC 1 cut(s) 865
Kzo9I GATC 6 cut(s) 329, 768, 772, 856, 915, 1128
LguI GCTCTTC 1 cut(s) 570
LmnI GCTCC 5 cut(s) 128, 167, 182, 242, 847
Lsp1109I GCAGC 6 cut(s) 40, 502, 608, 820, 837, 863
LweI GCATC 4 cut(s) 232, 655, 673, 788
MaeII ACGT 1 cut(s) 693
MaeIII GTNAC 2 cut(s) 284, 1151
MalI GATC 6 cut(s) 331, 770, 774, 858, 917, 1130
MboI GATC 6 cut(s) 329, 768, 772, 856, 915, 1128
MboII GAAGA 7 cut(s) 401, 479, 536, 557, 745, 917, 1138
MfeI CAATTG 2 cut(s) 263, 1080
MluCI AATT 8 cut(s) 263, 488, 718, 919, 1000, 1080, 1156, 1174
MlyI GAGTC 1 cut(s) 429
MmeI TCCRAC 3 cut(s) 81, 106, 1075
MnlI CCTC 8 cut(s) 97, 158, 167, 262, 269, 488, 768, 1023
MroNI GCCGGC 1 cut(s) 863
MroXI GAANNNNTTC 1 cut(s) 8
MseI TTAA 2 cut(s) 629, 887
MspI CCGG 5 cut(s) 209, 585, 622, 848, 864
MspR9I CCNGG 3 cut(s) 210, 623, 636
MunI CAATTG 2 cut(s) 263, 1080
Mva1269I GAATGC 1 cut(s) 958
MvaI CCWGG 1 cut(s) 636
MvnI CGCG 2 cut(s) 228, 876
MwoI GCNNNNNNNGC 7 cut(s) 182, 560, 738, 747, 848, 860, 873
NaeI GCCGGC 1 cut(s) 865
NciI CCSGG 2 cut(s) 210, 623
NdeI CATATG 1 cut(s) 813
NdeII GATC 6 cut(s) 329, 768, 772, 856, 915, 1128
NgoMIV GCCGGC 1 cut(s) 863
NlaIII CATG 5 cut(s) 346, 547, 896, 1044, 1152
NlaIV GGNNCC 2 cut(s) 175, 202
NmuCI GTSAC 1 cut(s) 1151
NsbI TGCGCA 1 cut(s) 1014
NspV TTCGAA 1 cut(s) 1179
PciSI GCTCTTC 1 cut(s) 570
PctI GAATGC 1 cut(s) 958
PdiI GCCGGC 1 cut(s) 865
PdmI GAANNNNTTC 1 cut(s) 8
PfeI GAWTC 5 cut(s) 116, 817, 933, 940, 1055
PflMI CCANNNNNTGG 1 cut(s) 1067
PkrI GCNGC 6 cut(s) 55, 517, 598, 810, 827, 853
PleI GAGTC 1 cut(s) 428
PpsI GAGTC 1 cut(s) 428
PsiI TTATAA 1 cut(s) 998
Psp6I CCWGG 1 cut(s) 634
PspFI CCCAGC 2 cut(s) 56, 170
PspGI CCWGG 1 cut(s) 634
PspN4I GGNNCC 2 cut(s) 175, 202
PspPI GGNCC 1 cut(s) 174
RsaI GTAC 2 cut(s) 71, 904
RsaNI GTAC 2 cut(s) 70, 903
SapI GCTCTTC 1 cut(s) 570
SaqAI TTAA 2 cut(s) 629, 887
SatI GCNGC 6 cut(s) 54, 516, 597, 809, 826, 852
Sau3AI GATC 6 cut(s) 329, 768, 772, 856, 915, 1128
Sau96I GGNCC 1 cut(s) 174
SchI GAGTC 1 cut(s) 429
ScrFI CCNGG 3 cut(s) 210, 623, 636
SfaNI GCATC 4 cut(s) 232, 655, 673, 788
SfcI CTRYAG 1 cut(s) 1086
SfuI TTCGAA 1 cut(s) 1179
Sse9I AATT 8 cut(s) 263, 488, 718, 919, 1000, 1080, 1156, 1174
SsiI CCGC 2 cut(s) 228, 325
StyD4I CCNGG 3 cut(s) 208, 621, 634
StyI CCWWGG 1 cut(s) 477
TaaI ACNGT 4 cut(s) 354, 611, 794, 966
TaiI ACGT 1 cut(s) 696
TaqI TCGA 4 cut(s) 310, 771, 1110, 1179
TaqII GACCGA 1 cut(s) 1128
TasI AATT 8 cut(s) 263, 488, 718, 919, 1000, 1080, 1156, 1174
TatI WGTACW 1 cut(s) 902
TfiI GAWTC 5 cut(s) 116, 817, 933, 940, 1055
Tru1I TTAA 2 cut(s) 629, 887
Tru9I TTAA 2 cut(s) 629, 887
TscAI CASTG 5 cut(s) 370, 614, 752, 797, 1022
TseFI GTSAC 1 cut(s) 1151
TseI GCWGC 6 cut(s) 53, 515, 596, 808, 825, 851
Tsp45I GTSAC 1 cut(s) 1151
TspDTI ATGAA 3 cut(s) 17, 96, 909
TspRI CASTG 5 cut(s) 370, 614, 752, 797, 1022
Van91I CCANNNNNTGG 1 cut(s) 1067
XapI RAATTY 3 cut(s) 718, 1000, 1174
XmnI GAANNNNTTC 1 cut(s) 8
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.