pycom14g01490

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0002063
Physical Location & Seq
Reverse (-)
1097080 .. 1098594
1515 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1356 bp
ATGGACACCAATCCACTGCAGCAACAACAACCCAACATGGACAGCCATTCAATCAGCATCTGGGAAGTGAACAAGGATCGGTTGGCCACCATGTACCAGATGATCTCAGAACCCCCAAAACTCCTAAGCAATGCAGCAGGCAAACCCTCCTGCTGCATCTTCAGAGTCCCACAGAGTCTTCTCGAGATAAACGGCAAGTCGTACAACCCCCACATCGTCTCCATCGGTCCATACCACAGAGGAGAGCCACGGGTCAAGATGATCGAACAGCACAAGTGGAAATATTTGGGTTCTTTGCTTGCAAGGACAGAGCCCAAAGGGTTGTCTTTAAAGGACTACTTGAAGGCCTTGGAGCCATTGGAACAAGAAGCTAGAGAGTGTTACTCAGAAAGAATAAATCTTACCACGGAAGAATTTTTGGAAATGTTGGTTCTTGATGGTTGTTTTATAATCGAATTGTTACGAAAAGTCGGCCTTTTGGTCCGGTTCGAGCGTGACGATCCTCTTGTCAACATGGTTTGGATAATCCCATTTTTGGTTAGGGATTTTCTTAGGCTCGAAAATCAAATACCCTACTTTGTTCTCCTGGCTTTGTTTGATCTCACAACTACGGAAGAACATAAAGAAAATGGGAAGTCATTGTCGTTGCTTGCCTTGGAATTTTTCAACAACCATATGCAGAGGACTGATCATGTCATTCAAAAGCACCACAATCTTACCGGTATGCATTTGCTCGATTTGTTGCGCTCAAGTGTTATACCGCCAGGCCATGAGGAGCCACGTAGTAGCAACACCATACCAACACATACCATCCATTGTGTCTCGAAGCTTCGCTGTGCAGGAATCAAGCTCAATCGGGGCAAGGGCGAAAGCTTCTTGGTGATTAAATTCAAGCGCGGAGTCATCGAAATGCCAACTATAACAGTCGACGATTTCATGAGTTCCTTCTTGCTCAACTGTGTGGCCTATGAGCAGTGCCACAAGTCAACTTCTAAGCACATCACCACCTACGCAACATTGCTCGATTGCCTTGTGAACACATACAAGGATGTTGAGTATTTGTGTGACCGTAACATCCTCGAGAATTACTTTGGGAACGATGGGGAAGTTGCTCGATTTATTAATAATTTGGGGAAGGATGTGGCTTTTGATATGGATGGGTGTTACTTGTCAAAGTTGTTCAATGACGTGCATCACTATTATGGGAATAGTTGGCATGTTCAATGGGCTAGCTTCAAGTATACTTATTTTGATACTCCATGGTCCTTCATATCTGCGTTTGCTGCTTTGATTTTGTTGATACTCACCTTGGCACAGACCTTTTACACTGTTATGAGTTACTATCCTCCACTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

452

Amino Acids

52.14

Weight (kDa)

6.31

Isoelectric Point (pI)

43.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 53 - 435 2.5e-123 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 449
AccI GTMKAC 2 cut(s) 927, 1241
AccII CGCG 1 cut(s) 897
AciI CCGC 2 cut(s) 761, 897
AclWI GGATC 2 cut(s) 84, 494
AcoI YGGCCR 1 cut(s) 84
AcsI RAATTY 3 cut(s) 413, 659, 887
AcuI CTGAAG 1 cut(s) 145
AfaI GTAC 2 cut(s) 95, 203
AfiI CCNNNNNNNGG 1 cut(s) 535
AgeI ACCGGT 1 cut(s) 719
AgsI TTSAA 8 cut(s) 51, 343, 667, 701, 892, 1183, 1223, 1237
AjiI CACGTC 1 cut(s) 1189
AjnI CCWGG 2 cut(s) 585, 763
AluBI AGCT 5 cut(s) 371, 829, 850, 873, 1233
AluI AGCT 5 cut(s) 371, 829, 850, 873, 1233
Alw26I GTCTC 2 cut(s) 223, 826
AlwI GGATC 2 cut(s) 84, 494
AlwNI CAGNNNCTG 1 cut(s) 60
Ama87I CYCGRG 2 cut(s) 182, 1079
AoxI GGCC 5 cut(s) 84, 345, 472, 766, 963
ApeKI GCWGC 4 cut(s) 19, 134, 153, 1283
ApoI RAATTY 3 cut(s) 413, 659, 887
AseI ATTAAT 1 cut(s) 1122
AsiGI ACCGGT 1 cut(s) 719
AspLEI GCGC 2 cut(s) 747, 897
AspS9I GGNCC 3 cut(s) 227, 481, 1263
AsuHPI GGTGA 3 cut(s) 892, 994, 1297
AsuNHI GCTAGC 1 cut(s) 1229
AvaI CYCGRG 2 cut(s) 182, 1079
AvaII GGWCC 3 cut(s) 227, 481, 1263
BalI TGGCCA 1 cut(s) 86
BanII GRGCYC 1 cut(s) 315
BbsI GAAGAC 1 cut(s) 170
BbvI GCAGC 4 cut(s) 31, 140, 146, 1270
BccI CCATC 5 cut(s) 230, 431, 818, 1094, 1151
BceAI ACGGC 1 cut(s) 208
BciT130I CCWGG 2 cut(s) 587, 765
BclI TGATCA 1 cut(s) 688
BcoDI GTCTC 2 cut(s) 223, 826
BfaI CTAG 2 cut(s) 372, 1230
BfmI CTRYAG 1 cut(s) 17
BisI GCNGC 4 cut(s) 20, 135, 154, 1284
BlsI GCNGC 4 cut(s) 21, 136, 155, 1285
Bme1390I CCNGG 2 cut(s) 587, 765
Bme18I GGWCC 3 cut(s) 227, 481, 1263
BmeT110I CYCGRG 2 cut(s) 182, 1079
BmgBI CACGTC 1 cut(s) 1189
BmgT120I GGNCC 3 cut(s) 227, 481, 1263
BmiI GGNNCC 2 cut(s) 354, 777
BmrFI CCNGG 2 cut(s) 587, 765
BmsI GCATC 3 cut(s) 66, 165, 1201
BmtI GCTAGC 1 cut(s) 1233
BpiI GAAGAC 1 cut(s) 170
BplI GAGNNNNNCTC 2 cut(s) 368, 400
Bpu10I CCTNAGC 1 cut(s) 125
BpuEI CTTGAG 1 cut(s) 733
BsaAI YACGTR 1 cut(s) 782
BsaJI CCNNGG 6 cut(s) 248, 348, 405, 654, 1259, 1308
BsaWI WCCGGW 2 cut(s) 483, 719
BsaXI ACNNNNNCTCC 2 cut(s) 203, 233
Bsc4I CCNNNNNNNGG 1 cut(s) 535
Bse118I RCCGGY 1 cut(s) 719
Bse3DI GCAATG 2 cut(s) 136, 1016
BseBI CCWGG 2 cut(s) 587, 765
BseDI CCNNGG 6 cut(s) 248, 348, 405, 654, 1259, 1308
BseGI GGATG 5 cut(s) 810, 1054, 1074, 1144, 1162
BseLI CCNNNNNNNGG 1 cut(s) 535
BseMI GCAATG 2 cut(s) 136, 1016
BseMII CTCAG 2 cut(s) 120, 399
BseRI GAGGAG 2 cut(s) 255, 788
BseXI GCAGC 4 cut(s) 31, 140, 146, 1270
BsgI GTGCAG 1 cut(s) 858
Bsh1236I CGCG 1 cut(s) 897
BshFI GGCC 5 cut(s) 86, 347, 474, 768, 965
BshTI ACCGGT 1 cut(s) 719
BsiHKCI CYCGRG 2 cut(s) 182, 1079
BsiSI CCGG 2 cut(s) 484, 720
BslFI GGGAC 1 cut(s) 152
BslI CCNNNNNNNGG 1 cut(s) 535
BsmAI GTCTC 2 cut(s) 223, 826
BsmBI CGTCTC 1 cut(s) 223
BsmFI GGGAC 1 cut(s) 152
BsnI GGCC 5 cut(s) 86, 347, 474, 768, 965
BsoBI CYCGRG 2 cut(s) 182, 1079
Bsp1286I GDGCHC 1 cut(s) 315
Bsp143I GATC 6 cut(s) 76, 102, 261, 499, 598, 688
Bsp19I CCATGG 1 cut(s) 1259
BspACI CCGC 2 cut(s) 761, 897
BspANI GGCC 5 cut(s) 86, 347, 474, 768, 965
BspCNI CTCAG 2 cut(s) 119, 398
BspFNI CGCG 1 cut(s) 897
BspHI TCATGA 1 cut(s) 936
BspLI GGNNCC 2 cut(s) 354, 777
BspMAI CTGCAG 1 cut(s) 21
BspOI GCTAGC 1 cut(s) 1233
BspPI GGATC 2 cut(s) 84, 494
BsrDI GCAATG 2 cut(s) 136, 1016
BsrFI RCCGGY 1 cut(s) 719
BssAI RCCGGY 1 cut(s) 719
BssECI CCNNGG 6 cut(s) 248, 348, 405, 654, 1259, 1308
BssMI GATC 6 cut(s) 76, 102, 261, 499, 598, 688
BssNAI GTATAC 1 cut(s) 1242
BssT1I CCWWGG 4 cut(s) 348, 654, 1259, 1308
Bst1107I GTATAC 1 cut(s) 1242
Bst2UI CCWGG 2 cut(s) 587, 765
Bst4CI ACNGT 4 cut(s) 925, 959, 1070, 1330
BstBAI YACGTR 1 cut(s) 782
BstC8I GCNNGC 4 cut(s) 139, 300, 651, 1231
BstDEI CTNAG 5 cut(s) 106, 125, 385, 551, 993
BstDSI CCRYGG 3 cut(s) 248, 405, 1259
BstF5I GGATG 5 cut(s) 810, 1054, 1074, 1144, 1162
BstFNI CGCG 1 cut(s) 897
BstHHI GCGC 2 cut(s) 747, 897
BstKTI GATC 6 cut(s) 79, 105, 264, 502, 601, 691
BstMAI GTCTC 2 cut(s) 223, 826
BstMBI GATC 6 cut(s) 76, 102, 261, 499, 598, 688
BstMWI GCNNNNNNNGC 1 cut(s) 1283
BstNI CCWGG 2 cut(s) 587, 765
BstNSI RCATGY 1 cut(s) 1220
BstSCI CCNGG 2 cut(s) 585, 763
BstSFI CTRYAG 1 cut(s) 17
BstUI CGCG 1 cut(s) 897
BstV1I GCAGC 4 cut(s) 31, 140, 146, 1270
BstV2I GAAGAC 1 cut(s) 170
BstZ17I GTATAC 1 cut(s) 1242
BsuRI GGCC 5 cut(s) 86, 347, 474, 768, 965
BtgI CCRYGG 3 cut(s) 248, 405, 1259
BtrI CACGTC 1 cut(s) 1189
BtsCI GGATG 5 cut(s) 810, 1054, 1074, 1144, 1162
BtsI GCAGTG 2 cut(s) 14, 980
BtsIMutI CAGTG 3 cut(s) 14, 980, 1326
Cac8I GCNNGC 4 cut(s) 139, 300, 651, 1231
CaiI CAGNNNCTG 1 cut(s) 60
CciI TCATGA 1 cut(s) 936
CfoI GCGC 2 cut(s) 747, 897
Cfr10I RCCGGY 1 cut(s) 719
Cfr13I GGNCC 3 cut(s) 227, 481, 1263
Csp6I GTAC 2 cut(s) 94, 202
CspAI ACCGGT 1 cut(s) 719
CviAII CATG 8 cut(s) 37, 91, 514, 692, 770, 937, 1217, 1260
CviQI GTAC 2 cut(s) 94, 202
DdeI CTNAG 5 cut(s) 106, 125, 385, 551, 993
DpnI GATC 6 cut(s) 78, 104, 263, 501, 600, 690
DpnII GATC 6 cut(s) 76, 102, 261, 499, 598, 688
DraI TTTAAA 1 cut(s) 330
EaeI YGGCCR 1 cut(s) 84
Eco130I CCWWGG 4 cut(s) 348, 654, 1259, 1308
Eco147I AGGCCT 1 cut(s) 347
Eco24I GRGCYC 1 cut(s) 315
Eco47I GGWCC 3 cut(s) 227, 481, 1263
Eco57I CTGAAG 1 cut(s) 145
Eco88I CYCGRG 2 cut(s) 182, 1079
EcoRII CCWGG 2 cut(s) 585, 763
EcoT14I CCWWGG 4 cut(s) 348, 654, 1259, 1308
EcoT22I ATGCAT 1 cut(s) 729
EcoT38I GRGCYC 1 cut(s) 315
ErhI CCWWGG 4 cut(s) 348, 654, 1259, 1308
Esp3I CGTCTC 1 cut(s) 223
FaeI CATG 8 cut(s) 40, 94, 517, 695, 773, 940, 1220, 1263
FalI AAGNNNNNCTT 4 cut(s) 323, 355, 459, 491
FaqI GGGAC 1 cut(s) 152
FatI CATG 8 cut(s) 36, 90, 513, 691, 769, 936, 1216, 1259
FauNDI CATATG 1 cut(s) 675
FbaI TGATCA 1 cut(s) 688
FblI GTMKAC 2 cut(s) 927, 1241
Fnu4HI GCNGC 4 cut(s) 20, 135, 154, 1284
FokI GGATG 5 cut(s) 797, 1061, 1061, 1151, 1169
FriOI GRGCYC 1 cut(s) 315
Fsp4HI GCNGC 4 cut(s) 20, 135, 154, 1284
FspBI CTAG 2 cut(s) 372, 1230
GlaI GCGC 2 cut(s) 746, 896
GluI GCNGC 4 cut(s) 20, 135, 154, 1284
HaeIII GGCC 5 cut(s) 86, 347, 474, 768, 965
HapII CCGG 2 cut(s) 484, 720
HhaI GCGC 2 cut(s) 747, 897
Hin1II CATG 8 cut(s) 40, 94, 517, 695, 773, 940, 1220, 1263
Hin6I GCGC 2 cut(s) 745, 895
HinP1I GCGC 2 cut(s) 745, 895
HincII GTYRAC 3 cut(s) 511, 928, 987
HindII GTYRAC 3 cut(s) 511, 928, 987
HindIII AAGCTT 2 cut(s) 827, 871
HinfI GANTC 4 cut(s) 165, 175, 843, 900
HpaII CCGG 2 cut(s) 484, 720
HphI GGTGA 3 cut(s) 892, 994, 1297
Hpy166II GTNNAC 6 cut(s) 70, 511, 928, 987, 1036, 1242
Hpy188I TCNGA 3 cut(s) 109, 164, 388
Hpy188III TCNNGA 7 cut(s) 182, 184, 256, 434, 823, 937, 1081
Hpy8I GTNNAC 6 cut(s) 70, 511, 928, 987, 1036, 1242
Hpy99I CGWCG 1 cut(s) 932
HpyAV CCTTC 4 cut(s) 337, 955, 1129, 1276
HpyCH4III ACNGT 4 cut(s) 925, 959, 1070, 1330
HpyCH4IV ACGT 2 cut(s) 781, 1188
HpyCH4V TGCA 8 cut(s) 19, 134, 156, 302, 679, 727, 839, 1192
HpyF10VI GCNNNNNNNGC 1 cut(s) 1283
HpyF3I CTNAG 5 cut(s) 106, 125, 385, 551, 993
HpySE526I ACGT 2 cut(s) 781, 1188
Hsp92II CATG 8 cut(s) 40, 94, 517, 695, 773, 940, 1220, 1263
HspAI GCGC 2 cut(s) 745, 895
Ksp22I TGATCA 1 cut(s) 688
Kzo9I GATC 6 cut(s) 76, 102, 261, 499, 598, 688
LmnI GCTCC 2 cut(s) 352, 775
Lsp1109I GCAGC 4 cut(s) 31, 140, 146, 1270
LweI GCATC 3 cut(s) 66, 165, 1201
MaeI CTAG 2 cut(s) 372, 1230
MaeII ACGT 2 cut(s) 781, 1188
MaeIII GTNAC 7 cut(s) 380, 459, 494, 1064, 1070, 1163, 1337
MalI GATC 6 cut(s) 78, 104, 263, 501, 600, 690
MboI GATC 6 cut(s) 76, 102, 261, 499, 598, 688
MboII GAAGA 4 cut(s) 151, 170, 422, 626
MhlI GDGCHC 1 cut(s) 315
MlsI TGGCCA 1 cut(s) 86
MluCI AATT 6 cut(s) 413, 455, 659, 887, 1084, 1126
MluNI TGGCCA 1 cut(s) 86
MlyI GAGTC 3 cut(s) 174, 184, 909
MnlI CCTC 7 cut(s) 157, 233, 513, 675, 766, 1088, 1356
Mox20I TGGCCA 1 cut(s) 86
Mph1103I ATGCAT 1 cut(s) 729
MscI TGGCCA 1 cut(s) 86
MseI TTAA 3 cut(s) 329, 885, 1122
MslI CAYNNNNRTG 3 cut(s) 908, 1200, 1331
Msp20I TGGCCA 1 cut(s) 86
MspI CCGG 2 cut(s) 484, 720
MspR9I CCNGG 2 cut(s) 587, 765
MvaI CCWGG 2 cut(s) 587, 765
MvnI CGCG 1 cut(s) 897
MwoI GCNNNNNNNGC 1 cut(s) 1283
NcoI CCATGG 1 cut(s) 1259
NdeI CATATG 1 cut(s) 675
NdeII GATC 6 cut(s) 76, 102, 261, 499, 598, 688
NheI GCTAGC 1 cut(s) 1229
NlaIII CATG 8 cut(s) 40, 94, 517, 695, 773, 940, 1220, 1263
NlaIV GGNNCC 2 cut(s) 354, 777
NmuCI GTSAC 2 cut(s) 494, 1064
NsiI ATGCAT 1 cut(s) 729
NspI RCATGY 1 cut(s) 1220
PaeR7I CTCGAG 2 cut(s) 182, 1079
PagI TCATGA 1 cut(s) 936
PceI AGGCCT 1 cut(s) 347
PcsI WCGNNNNNNNCGW 1 cut(s) 495
PfeI GAWTC 1 cut(s) 843
PinAI ACCGGT 1 cut(s) 719
PkrI GCNGC 4 cut(s) 21, 136, 155, 1285
PleI GAGTC 3 cut(s) 173, 183, 908
PpsI GAGTC 3 cut(s) 173, 183, 908
Ppu21I YACGTR 1 cut(s) 782
PshBI ATTAAT 1 cut(s) 1122
PsiI TTATAA 1 cut(s) 449
Psp6I CCWGG 2 cut(s) 585, 763
PspGI CCWGG 2 cut(s) 585, 763
PspN4I GGNNCC 2 cut(s) 354, 777
PspPI GGNCC 3 cut(s) 227, 481, 1263
PstI CTGCAG 1 cut(s) 21
PstNI CAGNNNCTG 1 cut(s) 60
RsaI GTAC 2 cut(s) 95, 203
RsaNI GTAC 2 cut(s) 94, 202
RseI CAYNNNNRTG 3 cut(s) 908, 1200, 1331
SalI GTCGAC 1 cut(s) 926
SaqAI TTAA 3 cut(s) 329, 885, 1122
SatI GCNGC 4 cut(s) 20, 135, 154, 1284
Sau3AI GATC 6 cut(s) 76, 102, 261, 499, 598, 688
Sau96I GGNCC 3 cut(s) 227, 481, 1263
SchI GAGTC 3 cut(s) 174, 184, 909
ScrFI CCNGG 2 cut(s) 587, 765
SduI GDGCHC 1 cut(s) 315
SfaNI GCATC 3 cut(s) 66, 165, 1201
SfcI CTRYAG 1 cut(s) 17
Sfr274I CTCGAG 2 cut(s) 182, 1079
SinI GGWCC 3 cut(s) 227, 481, 1263
SlaI CTCGAG 2 cut(s) 182, 1079
SmiMI CAYNNNNRTG 3 cut(s) 908, 1200, 1331
SmlI CTYRAG 3 cut(s) 182, 748, 1079
SmoI CTYRAG 3 cut(s) 182, 748, 1079
Sse9I AATT 6 cut(s) 413, 455, 659, 887, 1084, 1126
SseBI AGGCCT 1 cut(s) 347
SsiI CCGC 2 cut(s) 761, 897
SspI AATATT 1 cut(s) 284
SspMI CTAG 2 cut(s) 372, 1230
StuI AGGCCT 1 cut(s) 347
StyD4I CCNGG 2 cut(s) 585, 763
StyI CCWWGG 4 cut(s) 348, 654, 1259, 1308
TaaI ACNGT 4 cut(s) 925, 959, 1070, 1330
TaiI ACGT 2 cut(s) 784, 1191
TaqII GACCGA 1 cut(s) 215
TasI AATT 6 cut(s) 413, 455, 659, 887, 1084, 1126
TfiI GAWTC 1 cut(s) 843
Tru1I TTAA 3 cut(s) 329, 885, 1122
Tru9I TTAA 3 cut(s) 329, 885, 1122
TscAI CASTG 3 cut(s) 21, 980, 1333
TseFI GTSAC 2 cut(s) 494, 1064
TseI GCWGC 4 cut(s) 19, 134, 153, 1283
Tsp45I GTSAC 2 cut(s) 494, 1064
TspDTI ATGAA 2 cut(s) 925, 1258
TspGWI ACGGA 2 cut(s) 422, 626
TspRI CASTG 3 cut(s) 21, 980, 1333
VpaK11BI GGWCC 3 cut(s) 227, 481, 1263
VspI ATTAAT 1 cut(s) 1122
XapI RAATTY 3 cut(s) 413, 659, 887
XceI RCATGY 1 cut(s) 1220
XhoI CTCGAG 2 cut(s) 182, 1079
XmiI GTMKAC 2 cut(s) 927, 1241
XspI CTAG 2 cut(s) 372, 1230
Zsp2I ATGCAT 1 cut(s) 729
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.