pycom17g04730

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0001784
Physical Location & Seq
Forward (+)
3253767 .. 3254315
549 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 549 bp
ATGTCTTCCCAATCGCAACCCACAGGCCCTTCCACCGACCCTCCACCGAACACTGCTACTCAACCGACAACGTCCTTCACACTGCACGCACACGACGTCGACTCCGACAATACGTCCGTTGTACTAGCCCTACCAGTTACCCCAGCGATGGCAGAAGGAGGAGGACCACCCCAACCCAACAGTTTCCTCGACTCTTATTTTTATCCAAATCCCCCGCCCGCCGCTGCCTTGGCGGCTGTGGCGCGCCTGTCGTTGGTGACGGCCTTGGCTGGGGCGACCCAGGCTTCGATAGAGGCCTTGCCGAAGGTGACTCTGACGGAGGAAACGTCGGCGGAGTGCTGTATCTGCCTGAAGAAGTTGGAGGTCGGGGAGGAAGCCAAGGAGATGCCATGTGACCATAGGTTTCATCCGGTTTGCATTGATAGCTGGCTCGAGAGGCGTCCTTCCTGCCCGCTTTGCCGGTTTTCGATGCCTACGAGGGCGGAGGCGGCCGCACAAGGGCGAGGACATCAGACCAACAGGTGGGATTCGATTGAAATGGTTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

183

Amino Acids

19.43

Weight (kDa)

4.92

Isoelectric Point (pI)

63.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 111 - 154 1.7e-07 RING-H2 zinc finger domain
zf-RING_2 PF13639 112 - 154 7.9e-14 Ring finger domain
zf-RING_11 PF17123 112 - 140 1.3e-08 RING-like zinc finger
zf-C3HC4_2 PF13923 112 - 153 9.1e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 113 - 153 1.3e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 99
AccB7I CCANNNNNTGG 1 cut(s) 522
AccI GTMKAC 1 cut(s) 99
AccII CGCG 1 cut(s) 244
AciI CCGC 9 cut(s) 215, 219, 222, 233, 332, 452, 482, 488, 492
AcoI YGGCCR 1 cut(s) 489
AcuI CTGAAG 1 cut(s) 371
AcyI GRCGYC 2 cut(s) 96, 439
AfaI GTAC 1 cut(s) 123
AfiI CCNNNNNNNGG 5 cut(s) 148, 253, 270, 498, 522
AgsI TTSAA 1 cut(s) 536
AhdI GACNNNNNGTC 1 cut(s) 112
AjnI CCWGG 1 cut(s) 279
AluBI AGCT 1 cut(s) 426
AluI AGCT 1 cut(s) 426
Ama87I CYCGRG 1 cut(s) 431
AoxI GGCC 4 cut(s) 25, 261, 294, 489
ApeKI GCWGC 1 cut(s) 224
ArsI GACNNNNNNTTYG 2 cut(s) 268, 300
AscI GGCGCGCC 1 cut(s) 242
AspLEI GCGC 2 cut(s) 244, 246
AspS9I GGNCC 2 cut(s) 26, 164
AsuHPI GGTGA 2 cut(s) 268, 319
AvaI CYCGRG 1 cut(s) 431
AvaII GGWCC 1 cut(s) 164
BbvI GCAGC 1 cut(s) 211
BccI CCATC 1 cut(s) 142
BceAI ACGGC 1 cut(s) 276
BciT130I CCWGG 1 cut(s) 281
BfaI CTAG 1 cut(s) 125
BglI GCCNNNNNGGC 1 cut(s) 233
BisI GCNGC 5 cut(s) 222, 225, 234, 489, 492
BlsI GCNGC 5 cut(s) 223, 226, 235, 490, 493
Bme1390I CCNGG 1 cut(s) 281
Bme18I GGWCC 1 cut(s) 164
BmeRI GACNNNNNGTC 1 cut(s) 112
BmeT110I CYCGRG 1 cut(s) 431
BmgT120I GGNCC 2 cut(s) 26, 164
BmrFI CCNGG 1 cut(s) 281
BmsI GCATC 2 cut(s) 375, 459
BsaHI GRCGYC 2 cut(s) 96, 439
BsaJI CCNNGG 4 cut(s) 228, 264, 279, 378
BsaWI WCCGGW 1 cut(s) 409
BsaXI ACNNNNNCTCC 6 cut(s) 25, 55, 86, 116, 311, 341
Bsc4I CCNNNNNNNGG 5 cut(s) 148, 253, 270, 498, 522
Bse118I RCCGGY 1 cut(s) 459
Bse1I ACTGG 1 cut(s) 134
BseBI CCWGG 1 cut(s) 281
BseDI CCNNGG 4 cut(s) 228, 264, 279, 378
BseGI GGATG 1 cut(s) 406
BseLI CCNNNNNNNGG 5 cut(s) 148, 253, 270, 498, 522
BseNI ACTGG 1 cut(s) 134
BsePI GCGCGC 1 cut(s) 242
BseRI GAGGAG 1 cut(s) 174
BseX3I CGGCCG 1 cut(s) 489
BseXI GCAGC 1 cut(s) 211
BseYI CCCAGC 2 cut(s) 142, 269
BsgI GTGCAG 1 cut(s) 68
Bsh1236I CGCG 1 cut(s) 244
Bsh1285I CGRYCG 1 cut(s) 492
BshFI GGCC 4 cut(s) 27, 263, 296, 491
BsiEI CGRYCG 1 cut(s) 492
BsiHKCI CYCGRG 1 cut(s) 431
BsiSI CCGG 2 cut(s) 410, 460
BslI CCNNNNNNNGG 5 cut(s) 148, 253, 270, 498, 522
BsnI GGCC 4 cut(s) 27, 263, 296, 491
BsoBI CYCGRG 1 cut(s) 431
BspACI CCGC 9 cut(s) 215, 219, 222, 233, 332, 452, 482, 488, 492
BspANI GGCC 4 cut(s) 27, 263, 296, 491
BspFNI CGCG 1 cut(s) 244
BsrFI RCCGGY 1 cut(s) 459
BsrI ACTGG 1 cut(s) 134
BssAI RCCGGY 1 cut(s) 459
BssECI CCNNGG 4 cut(s) 228, 264, 279, 378
BssHII GCGCGC 1 cut(s) 242
BssNI GRCGYC 2 cut(s) 96, 439
BssT1I CCWWGG 3 cut(s) 228, 264, 378
Bst2UI CCWGG 1 cut(s) 281
Bst4CI ACNGT 1 cut(s) 182
BstACI GRCGYC 2 cut(s) 96, 439
BstC8I GCNNGC 5 cut(s) 87, 219, 244, 428, 452
BstF5I GGATG 1 cut(s) 406
BstFNI CGCG 1 cut(s) 244
BstHHI GCGC 2 cut(s) 244, 246
BstMCI CGRYCG 1 cut(s) 492
BstMWI GCNNNNNNNGC 9 cut(s) 230, 233, 239, 281, 345, 423, 436, 456, 488
BstNI CCWGG 1 cut(s) 281
BstSCI CCNGG 1 cut(s) 279
BstUI CGCG 1 cut(s) 244
BstV1I GCAGC 1 cut(s) 211
BstZI CGGCCG 1 cut(s) 489
BsuRI GGCC 4 cut(s) 27, 263, 296, 491
BtgZI GCGATG 1 cut(s) 161
BtsCI GGATG 1 cut(s) 406
BtsI GCAGTG 2 cut(s) 51, 80
BtsIMutI CAGTG 2 cut(s) 51, 80
Cac8I GCNNGC 5 cut(s) 87, 219, 244, 428, 452
CciNI GCGGCCGC 1 cut(s) 489
CfoI GCGC 2 cut(s) 244, 246
Cfr10I RCCGGY 1 cut(s) 459
Cfr13I GGNCC 2 cut(s) 26, 164
CseI GACGC 1 cut(s) 428
Csp6I GTAC 1 cut(s) 122
CviAII CATG 1 cut(s) 390
CviQI GTAC 1 cut(s) 122
DriI GACNNNNNGTC 1 cut(s) 112
EaeI YGGCCR 1 cut(s) 489
EagI CGGCCG 1 cut(s) 489
Eam1105I GACNNNNNGTC 1 cut(s) 112
EciI GGCGGA 2 cut(s) 347, 497
EclXI CGGCCG 1 cut(s) 489
Eco130I CCWWGG 3 cut(s) 228, 264, 378
Eco147I AGGCCT 1 cut(s) 296
Eco47I GGWCC 1 cut(s) 164
Eco52I CGGCCG 1 cut(s) 489
Eco57I CTGAAG 1 cut(s) 371
Eco88I CYCGRG 1 cut(s) 431
EcoO109I RGGNCCY 1 cut(s) 26
EcoRII CCWGG 1 cut(s) 279
EcoT14I CCWWGG 3 cut(s) 228, 264, 378
ErhI CCWWGG 3 cut(s) 228, 264, 378
FaeI CATG 1 cut(s) 393
FaiI YATR 2 cut(s) 391, 399
FatI CATG 1 cut(s) 389
FauI CCCGC 3 cut(s) 222, 226, 459
FblI GTMKAC 1 cut(s) 99
Fnu4HI GCNGC 5 cut(s) 222, 225, 234, 489, 492
FokI GGATG 1 cut(s) 393
Fsp4HI GCNGC 5 cut(s) 222, 225, 234, 489, 492
FspBI CTAG 1 cut(s) 125
GlaI GCGC 2 cut(s) 243, 245
GluI GCNGC 5 cut(s) 222, 225, 234, 489, 492
GsaI CCCAGC 2 cut(s) 146, 273
HaeIII GGCC 4 cut(s) 27, 263, 296, 491
HapII CCGG 2 cut(s) 410, 460
HgaI GACGC 1 cut(s) 428
HhaI GCGC 2 cut(s) 244, 246
Hin1I GRCGYC 2 cut(s) 96, 439
Hin1II CATG 1 cut(s) 393
Hin6I GCGC 2 cut(s) 242, 244
HinP1I GCGC 2 cut(s) 242, 244
HincII GTYRAC 1 cut(s) 100
HindII GTYRAC 1 cut(s) 100
HinfI GANTC 4 cut(s) 101, 191, 310, 527
HpaII CCGG 2 cut(s) 410, 460
HphI GGTGA 2 cut(s) 268, 319
Hpy166II GTNNAC 1 cut(s) 100
Hpy188I TCNGA 3 cut(s) 106, 315, 513
Hpy188III TCNNGA 1 cut(s) 433
Hpy8I GTNNAC 1 cut(s) 100
Hpy99I CGWCG 3 cut(s) 98, 101, 331
HpyAV CCTTC 5 cut(s) 39, 85, 149, 298, 453
HpyCH4III ACNGT 1 cut(s) 182
HpyCH4IV ACGT 4 cut(s) 71, 96, 113, 326
HpyCH4V TGCA 2 cut(s) 85, 417
HpyF10VI GCNNNNNNNGC 9 cut(s) 230, 233, 239, 281, 345, 423, 436, 456, 488
HpySE526I ACGT 4 cut(s) 71, 96, 113, 326
Hsp92I GRCGYC 2 cut(s) 96, 439
Hsp92II CATG 1 cut(s) 393
HspAI GCGC 2 cut(s) 242, 244
Lsp1109I GCAGC 1 cut(s) 211
LweI GCATC 2 cut(s) 375, 459
MaeI CTAG 1 cut(s) 125
MaeII ACGT 4 cut(s) 71, 96, 113, 326
MaeIII GTNAC 4 cut(s) 136, 256, 307, 392
MboII GAAGA 1 cut(s) 364
MlyI GAGTC 3 cut(s) 95, 185, 304
MmeI TCCRAC 2 cut(s) 129, 339
MspA1I CMGCKG 1 cut(s) 224
MspI CCGG 2 cut(s) 410, 460
MspR9I CCNGG 1 cut(s) 281
MvaI CCWGG 1 cut(s) 281
MvnI CGCG 1 cut(s) 244
MwoI GCNNNNNNNGC 9 cut(s) 230, 233, 239, 281, 345, 423, 436, 456, 488
NlaIII CATG 1 cut(s) 393
NmuCI GTSAC 3 cut(s) 256, 307, 392
NotI GCGGCCGC 1 cut(s) 489
PaeR7I CTCGAG 1 cut(s) 431
PalAI GGCGCGCC 1 cut(s) 242
PauI GCGCGC 1 cut(s) 242
PceI AGGCCT 1 cut(s) 296
PcsI WCGNNNNNNNCGW 4 cut(s) 93, 102, 323, 473
PfeI GAWTC 1 cut(s) 527
PflFI GACNNNGTC 1 cut(s) 70
PflMI CCANNNNNTGG 1 cut(s) 522
PkrI GCNGC 5 cut(s) 223, 226, 235, 490, 493
PleI GAGTC 3 cut(s) 95, 185, 304
PpsI GAGTC 3 cut(s) 95, 185, 304
Psp6I CCWGG 1 cut(s) 279
PspFI CCCAGC 2 cut(s) 142, 269
PspGI CCWGG 1 cut(s) 279
PspPI GGNCC 2 cut(s) 26, 164
PsyI GACNNNGTC 1 cut(s) 70
PteI GCGCGC 1 cut(s) 242
RsaI GTAC 1 cut(s) 123
RsaNI GTAC 1 cut(s) 122
SalI GTCGAC 1 cut(s) 98
SatI GCNGC 5 cut(s) 222, 225, 234, 489, 492
Sau96I GGNCC 2 cut(s) 26, 164
SchI GAGTC 3 cut(s) 95, 185, 304
ScrFI CCNGG 1 cut(s) 281
SetI ASST 9 cut(s) 74, 99, 116, 309, 329, 366, 404, 428, 524
SfaNI GCATC 2 cut(s) 375, 459
Sfr274I CTCGAG 1 cut(s) 431
SgsI GGCGCGCC 1 cut(s) 242
SinI GGWCC 1 cut(s) 164
SlaI CTCGAG 1 cut(s) 431
SmlI CTYRAG 1 cut(s) 431
SmoI CTYRAG 1 cut(s) 431
SseBI AGGCCT 1 cut(s) 296
SsiI CCGC 9 cut(s) 215, 219, 222, 233, 332, 452, 482, 488, 492
SspMI CTAG 1 cut(s) 125
StuI AGGCCT 1 cut(s) 296
StyD4I CCNGG 1 cut(s) 279
StyI CCWWGG 3 cut(s) 228, 264, 378
TaaI ACNGT 1 cut(s) 182
TaiI ACGT 4 cut(s) 74, 99, 116, 329
TaqI TCGA 6 cut(s) 99, 189, 287, 432, 467, 530
TatI WGTACW 1 cut(s) 121
TauI GCSGC 4 cut(s) 224, 236, 491, 494
TfiI GAWTC 1 cut(s) 527
TscAI CASTG 2 cut(s) 58, 87
TseFI GTSAC 3 cut(s) 256, 307, 392
TseI GCWGC 1 cut(s) 224
Tsp45I GTSAC 3 cut(s) 256, 307, 392
TspDTI ATGAA 1 cut(s) 395
TspGWI ACGGA 2 cut(s) 106, 332
TspRI CASTG 2 cut(s) 58, 87
Tth111I GACNNNGTC 1 cut(s) 70
Van91I CCANNNNNTGG 1 cut(s) 522
VpaK11BI GGWCC 1 cut(s) 164
XhoI CTCGAG 1 cut(s) 431
XmiI GTMKAC 1 cut(s) 99
XspI CTAG 1 cut(s) 125
ZraI GACGTC 1 cut(s) 97
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.