Belongs to the GST superfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 53 |
| AciI | CCGC | 1 cut(s) | 294 |
| AclWI | GGATC | 2 cut(s) | 220, 272 |
| AcsI | RAATTY | 2 cut(s) | 314, 508 |
| AcuI | CTGAAG | 1 cut(s) | 107 |
| AfaI | GTAC | 1 cut(s) | 416 |
| AgsI | TTSAA | 4 cut(s) | 122, 155, 233, 242 |
| AluBI | AGCT | 3 cut(s) | 8, 37, 91 |
| AluI | AGCT | 3 cut(s) | 8, 37, 91 |
| Alw21I | GWGCWC | 1 cut(s) | 195 |
| Alw44I | GTGCAC | 1 cut(s) | 191 |
| AlwI | GGATC | 2 cut(s) | 220, 272 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 212 |
| AoxI | GGCC | 1 cut(s) | 396 |
| ApaLI | GTGCAC | 1 cut(s) | 191 |
| ApeKI | GCWGC | 1 cut(s) | 296 |
| ApoI | RAATTY | 2 cut(s) | 314, 508 |
| AsuHPI | GGTGA | 1 cut(s) | 332 |
| BaeGI | GKGCMC | 2 cut(s) | 85, 195 |
| Bbv12I | GWGCWC | 1 cut(s) | 195 |
| BbvI | GCAGC | 1 cut(s) | 283 |
| BccI | CCATC | 3 cut(s) | 122, 346, 483 |
| BglII | AGATCT | 1 cut(s) | 124 |
| BisI | GCNGC | 2 cut(s) | 294, 297 |
| BlsI | GCNGC | 2 cut(s) | 295, 298 |
| BmcAI | AGTACT | 1 cut(s) | 416 |
| BmiI | GGNNCC | 1 cut(s) | 180 |
| BsaJI | CCNNGG | 1 cut(s) | 399 |
| Bse1I | ACTGG | 1 cut(s) | 182 |
| BseDI | CCNNGG | 1 cut(s) | 399 |
| BseGI | GGATG | 1 cut(s) | 494 |
| BseNI | ACTGG | 1 cut(s) | 182 |
| BseRI | GAGGAG | 1 cut(s) | 440 |
| BseSI | GKGCMC | 2 cut(s) | 85, 195 |
| BseXI | GCAGC | 1 cut(s) | 283 |
| BsgI | GTGCAG | 1 cut(s) | 282 |
| BshFI | GGCC | 1 cut(s) | 398 |
| BsiHKAI | GWGCWC | 1 cut(s) | 195 |
| BsnI | GGCC | 1 cut(s) | 398 |
| Bsp1286I | GDGCHC | 2 cut(s) | 85, 195 |
| Bsp143I | GATC | 3 cut(s) | 124, 225, 277 |
| BspACI | CCGC | 1 cut(s) | 294 |
| BspANI | GGCC | 1 cut(s) | 398 |
| BspLI | GGNNCC | 1 cut(s) | 180 |
| BspPI | GGATC | 2 cut(s) | 220, 272 |
| BspQI | GCTCTTC | 1 cut(s) | 44 |
| BsrI | ACTGG | 1 cut(s) | 182 |
| BssECI | CCNNGG | 1 cut(s) | 399 |
| BssMI | GATC | 3 cut(s) | 124, 225, 277 |
| BssT1I | CCWWGG | 1 cut(s) | 399 |
| Bst4CI | ACNGT | 1 cut(s) | 362 |
| Bst6I | CTCTTC | 2 cut(s) | 44, 417 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 479 |
| BstC8I | GCNNGC | 2 cut(s) | 301, 495 |
| BstF5I | GGATG | 1 cut(s) | 494 |
| BstKTI | GATC | 3 cut(s) | 127, 228, 280 |
| BstMBI | GATC | 3 cut(s) | 124, 225, 277 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 479 |
| BstSLI | GKGCMC | 2 cut(s) | 85, 195 |
| BstV1I | GCAGC | 1 cut(s) | 283 |
| BstX2I | RGATCY | 2 cut(s) | 124, 277 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 22, 345 |
| BstYI | RGATCY | 2 cut(s) | 124, 277 |
| BsuRI | GGCC | 1 cut(s) | 398 |
| BtsCI | GGATG | 1 cut(s) | 494 |
| BtsIMutI | CAGTG | 3 cut(s) | 83, 189, 367 |
| Cac8I | GCNNGC | 2 cut(s) | 301, 495 |
| CaiI | CAGNNNCTG | 1 cut(s) | 212 |
| Csp6I | GTAC | 1 cut(s) | 415 |
| CviAII | CATG | 3 cut(s) | 48, 249, 519 |
| CviJI | RGCY | 8 cut(s) | 8, 37, 91, 141, 293, 353, 398, 493 |
| CviKI_1 | RGCY | 8 cut(s) | 8, 37, 91, 141, 293, 353, 398, 493 |
| CviQI | GTAC | 1 cut(s) | 415 |
| DpnI | GATC | 3 cut(s) | 126, 227, 279 |
| DpnII | GATC | 3 cut(s) | 124, 225, 277 |
| Eam1104I | CTCTTC | 2 cut(s) | 44, 417 |
| EarI | CTCTTC | 2 cut(s) | 44, 417 |
| Eco130I | CCWWGG | 1 cut(s) | 399 |
| Eco57I | CTGAAG | 1 cut(s) | 107 |
| EcoT14I | CCWWGG | 1 cut(s) | 399 |
| ErhI | CCWWGG | 1 cut(s) | 399 |
| FaeI | CATG | 3 cut(s) | 51, 252, 522 |
| FaiI | YATR | 6 cut(s) | 49, 171, 250, 462, 504, 520 |
| FatI | CATG | 3 cut(s) | 47, 248, 518 |
| FblI | GTMKAC | 1 cut(s) | 53 |
| Fnu4HI | GCNGC | 2 cut(s) | 294, 297 |
| FokI | GGATG | 1 cut(s) | 501 |
| Fsp4HI | GCNGC | 2 cut(s) | 294, 297 |
| GluI | GCNGC | 2 cut(s) | 294, 297 |
| HaeIII | GGCC | 1 cut(s) | 398 |
| Hin1II | CATG | 3 cut(s) | 51, 252, 522 |
| HinfI | GANTC | 1 cut(s) | 215 |
| HphI | GGTGA | 1 cut(s) | 332 |
| Hpy166II | GTNNAC | 2 cut(s) | 54, 193 |
| Hpy188I | TCNGA | 2 cut(s) | 109, 274 |
| Hpy188III | TCNNGA | 1 cut(s) | 284 |
| Hpy8I | GTNNAC | 2 cut(s) | 54, 193 |
| HpyAV | CCTTC | 3 cut(s) | 169, 359, 388 |
| HpyCH4III | ACNGT | 1 cut(s) | 362 |
| HpyCH4IV | ACGT | 1 cut(s) | 408 |
| HpyCH4V | TGCA | 5 cut(s) | 193, 299, 473, 482, 497 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 479 |
| HpySE526I | ACGT | 1 cut(s) | 408 |
| Hsp92II | CATG | 3 cut(s) | 51, 252, 522 |
| Kzo9I | GATC | 3 cut(s) | 124, 225, 277 |
| LguI | GCTCTTC | 1 cut(s) | 44 |
| LpnPI | CCDG | 3 cut(s) | 64, 177, 195 |
| Lsp1109I | GCAGC | 1 cut(s) | 283 |
| MaeII | ACGT | 1 cut(s) | 408 |
| MalI | GATC | 3 cut(s) | 126, 227, 279 |
| MboI | GATC | 3 cut(s) | 124, 225, 277 |
| MboII | GAAGA | 7 cut(s) | 31, 134, 143, 254, 287, 434, 451 |
| MflI | RGATCY | 2 cut(s) | 124, 277 |
| MhlI | GDGCHC | 2 cut(s) | 85, 195 |
| MluCI | AATT | 3 cut(s) | 314, 443, 508 |
| MnlI | CCTC | 4 cut(s) | 63, 291, 373, 418 |
| MseI | TTAA | 2 cut(s) | 357, 431 |
| MslI | CAYNNNNRTG | 1 cut(s) | 487 |
| MspA1I | CMGCKG | 1 cut(s) | 296 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 479 |
| NdeII | GATC | 3 cut(s) | 124, 225, 277 |
| NlaIII | CATG | 3 cut(s) | 51, 252, 522 |
| NlaIV | GGNNCC | 1 cut(s) | 180 |
| PciSI | GCTCTTC | 1 cut(s) | 44 |
| PfeI | GAWTC | 1 cut(s) | 215 |
| PkrI | GCNGC | 2 cut(s) | 295, 298 |
| PspN4I | GGNNCC | 1 cut(s) | 180 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 398, 430 |
| PstNI | CAGNNNCTG | 1 cut(s) | 212 |
| PsuI | RGATCY | 2 cut(s) | 124, 277 |
| RsaI | GTAC | 1 cut(s) | 416 |
| RsaNI | GTAC | 1 cut(s) | 415 |
| RseI | CAYNNNNRTG | 1 cut(s) | 487 |
| SapI | GCTCTTC | 1 cut(s) | 44 |
| SaqAI | TTAA | 2 cut(s) | 357, 431 |
| SatI | GCNGC | 2 cut(s) | 294, 297 |
| Sau3AI | GATC | 3 cut(s) | 124, 225, 277 |
| ScaI | AGTACT | 1 cut(s) | 416 |
| SduI | GDGCHC | 2 cut(s) | 85, 195 |
| SetI | ASST | 8 cut(s) | 10, 39, 93, 180, 333, 384, 388, 411 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 487 |
| Sse9I | AATT | 3 cut(s) | 314, 443, 508 |
| SsiI | CCGC | 1 cut(s) | 294 |
| StyI | CCWWGG | 1 cut(s) | 399 |
| TaaI | ACNGT | 1 cut(s) | 362 |
| TaiI | ACGT | 1 cut(s) | 411 |
| TasI | AATT | 3 cut(s) | 314, 443, 508 |
| TatI | WGTACW | 1 cut(s) | 414 |
| TauI | GCSGC | 1 cut(s) | 296 |
| TfiI | GAWTC | 1 cut(s) | 215 |
| Tru1I | TTAA | 2 cut(s) | 357, 431 |
| Tru9I | TTAA | 2 cut(s) | 357, 431 |
| TscAI | CASTG | 3 cut(s) | 90, 189, 367 |
| TseI | GCWGC | 1 cut(s) | 296 |
| TspDTI | ATGAA | 2 cut(s) | 158, 339 |
| TspRI | CASTG | 3 cut(s) | 90, 189, 367 |
| VneI | GTGCAC | 1 cut(s) | 191 |
| XapI | RAATTY | 2 cut(s) | 314, 508 |
| XmiI | GTMKAC | 1 cut(s) | 53 |
| ZrmI | AGTACT | 1 cut(s) | 416 |