Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0001033

Physical Location & Seq

Genomic Coordinates Reverse (-)

33744957 .. 33752594

Gene Structure 7638 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2421 bp

ATGCAGGAAAGCACACACGTGGAAAACGATGACGGCTATTACAGCGGTCGTGAAGAAGATGACAATGTCCGCCATGGTGAAAGGAAAACGAGAGACTTTACCAAACTAGAATTGAAGCCTGACCACCCTAATCGGCCTTTATGGGCTTGTCCAGATGGCCGGATATTCCTGGAAACTTTCTCGGCTTTGTACAAACAAGCTTATGATTTCCTCATTGCCATTGCGGAACCCGTTTGCAGGCAAGAGTGGATGCACGAGTATAACTTGACCCCGCATTCTTTATATGCGGCTGTCTCAGTTGGCCTTGAAACCGAAACAATTATTTCTGTTCTAAATAAGCTGTCAAAGACCAAGCTCCCCAAAGAGATTATTGATTTCATACATGGTTCTACTGCTAATTATGGCAAAGTGAAGCTCGTGCTTAAGAAGAATCGGTATTTTGTCGAGTCGCCATATCCAGAGGCAGGATTTTATGCCTCCCTTAATTACCTTTTTCAGTTTACATTAGTACTGAAGACATTGCTTAAGGACAACTTTATTGCAGAATCAAGGATTACTCCTGAGGGTCCTCATGGAAATGAAGATTTTACAATTAGCACAACGATGGGTGAAATTGGAGCTGGTCCTAGTGATTTGCTAAATGAAGCAGAACTGGCAGCTGTGGCTGAAGAAAAAGAAACTCATGCATTTGAAATTGATCCTTCAAAGGTTGAGAATGTTAAGCAACGTTGCTTGCCAGATTCTTTGAACTATCCTATGTTGGAGGAGTATGACTTCAAAAATGATACAGTTAACCCTGATCTTGATATGGAACTAAAGCCTCAAGCGCAACCCCGACCGTATCAAGAGAAGAGCTTGAGTAAAATGTTTGGGAATGGGAGGGCAAGATCTGGCATTATTGTACTGCCTTGTGGTGCTGGAAAATCTCTAGTTGGTGTTTCTGCAGCCTCTCGAATAAGAAAGAGCTGCCTTTGTTTAGCAACAAATGCCGTTTCCGTTGATCAGTGGGCTTTTCAGTTTAAGTTGTGGTCAACTATCCGAGATGACCAAATTTGTCGTTTCACATCTGATAGTAAAGAAAGAGTCCGTGGAAATGCTGGGGTGGTTGTGACAACATATAACATGATTGGTTTTGGTGGAAAGAGGTCCGAAGAATCTGAAAAGATTATTGAAGAAATAAGAAACAGAGAATGGGGATTGCTTCTTATGGATGAGGTGATTATATATATGATTTTGTGCATGTTGTTCCTGCTGAAATGTTTCGAAATGTCATCAAATTTACTAAATCACATTGCAAGCTTGGGCTCACGGGTGAGTCGGATACAGCTTTGGTCCATTGCTATACAACTTACCCTTGATCTTTTTGTTTTTGGATATCCAAATTCACGCATCAGCACAATTGCAGCCACACTGGTGAGAGAGGATGAGAAAATCACAGATCTGAACTTCCTTATTGGTCCCAAATTGTATGAAGCAAACTGGTTGGATTTGGTTAAAGGCGGATTTATTGCTAATGTACAGTGTGCTGAAGTATGGTGTCCTATGACAAAAGAGTTTTTCCTGGAATATTTGAAGAGGGAAAACTCAAAGAAAAAGCAGGCACTTTATGTAATGAATCCAAATAAGTTTAGGGCATGCGAGTTTCTTATACGGTTTCATGAAGAGCACCGTGGTGATAAAATTATTGTATTTGCCGATAATCTATTTGCACTTACAGAGTATGCAATGAAACTTCGGAAACCTATGATCTATGGTGCTACCAGCCATGCTGAGAGGACAAAAATTCTACAAGCTTTCAAAACAAGTCGGGACGTAAACACCGTATTCCTGTCTAAGGTGGGTGATAATTCCATAGACATTCCTGAGGCAAATGTGATTATTCAGATCTCATCACATGCTGGTTCAAGACGTCAAGAAGCACAGCGTCTTGGACGTATTTTGAGGGCTAAGGGTAGACCTCAGGATCGGATTGCAGGAGGTAAAGAGGAGTACAATGCGTTCTTTTATTCTCTTGTGTCGACTGATACTCAGGAGATGTACTTTTCAACTAAACGCCAGCAGTTTTTGATTGATCAAGGTTATAGTTTTAAGGTTATTACAAGCTTGCCCCCTCCTGAATCAGGATCTGATTTGAGCTATCACCGTCTTGATGACCAACTGGCACTACTTTCAAAGGTGTTGAGTGTTGGTGATGATAAAGTTGGCTTAGAGACACTAGAAGAAGACCCTGATGAGATTGCCCTTCAGAAAGCACGTCGCTCAACAGGATCCATGACTTTGATGTCAGGCGCCAAGGGGATGGTTTACATGGAATATAGTACTGAAACGCAGAAACACAAGCATGGCAAAGCACAGATTAAGAGTACTAAGCCCAGGGATCCGGCAAAGAGACATTATTTGTTTAAACAACGCCGTGGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0008150 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0010224 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0050896

KEGG Pathways

Metabolic & Signaling

ko03022 ko03420 map03022 map03420

Pfam Domains

Protein Families

ERCC3_RAD25_C Helicase_C_3 ResIII

Protein Analysis

806

Amino Acids

91.74

Weight (kDa)

7.25

Isoelectric Point (pI)

37.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Helicase_C_3	PF13625	47 - 154	8.4e-29	Helicase conserved C-terminal domain
ResIII	PF04851	277 - 406	1e-08	Type III restriction enzyme, res subunit
ERCC3_RAD25_C	PF16203	497 - 743	2.3e-115	ERCC3/RAD25/XPB C-terminal helicase
Helicase_C	PF00271	547 - 649	3.6e-13	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0001033)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G41360 AT5G41360 AT5G41370
fragaria_vesca	FvH4_5g02840 FvH4_5g02840 FvH4_5g02840 FvH4_5g02840 FvH4_5g02840 FvH4_5g19420 FvH4_5g19430 FvH4_7g08220 FvH4_7g08220
malus_domestica	MD06G1087900.v1.1
prunus_persica	Prupe.5G104000_v2.0.a1
pyrus_communis	pycom06g08400
rosa_chinensis	RchiOBHm_Chr1g0341611 RchiOBHm_Chr1g0341621 RchiOBHm_Chr1g0341861 RchiOBHm_Chr7g0197061 RchiOBHm_Chr7g0203931
rosa_laevigata	RLG00000003497 RLG00000003962 RLG00000029105 RLG00000029132
rosa_multiflora	Rmu_co8328521.1_g000001 Rmu_co8329503.1_g000001 Rmu_sc0002302.1_g000034 Rmu_sc0002322.1_g000070 Rmu_sc0003411.1_g000004 Rmu_sc0013871.1_g000001 Rmu_sc0032022.1_g000001
rosa_roxburghii	Rroxscaffold_3G00258690 Rroxscaffold_4G00312270 Rroxscaffold_4G00312590
rosa_rugosa	Rorug01G0154800.1 Rorug07G0037400 Rorug07G0079500
rosa_samantha	Rh1AG168500 Rh1AG170500 Rh1BG136800 Rh1BG138300 Rh1BG138400 Rh1CG156100 Rh1CG158700 Rh1DG170300 Rh1DG170600 Rh1DG170700 Rh7AG162300 Rh7AG209400 Rh7AG209500 Rh7BG164400 Rh7BG208200 Rh7BG208300 Rh7CG170500 Rh7CG221900 Rh7DG163800 Rh7DG216500
rosa_wichuraiana	Rw1G014160 Rw1G014180 Rw7G014170 Rw7G018250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2281
AatII	GACGTC	1 cut(s)	1912
AccB1I	GGYRCC	1 cut(s)	2288
AccI	GTMKAC	2 cut(s)	1954, 2018
AciI	CCGC	6 cut(s)	45, 70, 224, 272, 287, 1500
AclI	AACGTT	1 cut(s)	727
AclWI	GGATC	7 cut(s)	692, 1971, 2131, 2262, 2275, 2372, 2385
AcoI	YGGCCR	1 cut(s)	157
AcsI	RAATTY	4 cut(s)	1050, 1276, 1381, 1782
AcuI	CTGAAG	4 cut(s)	533, 687, 1548, 2228
AcvI	CACGTG	1 cut(s)	19
AcyI	GRCGYC	2 cut(s)	1909, 2289
AfaI	GTAC	8 cut(s)	191, 510, 903, 1518, 1991, 2039, 2320, 2365
AfiI	CCNNNNNNNGG	4 cut(s)	237, 464, 1834, 2120
AflII	CTTAAG	2 cut(s)	422, 524
AflIII	ACRYGT	1 cut(s)	16
AjiI	CACGTC	1 cut(s)	2255
AjnI	CCWGG	3 cut(s)	168, 1560, 2372
AleI	CACNNNNGTG	3 cut(s)	17, 1412, 1671
Alw21I	GWGCWC	1 cut(s)	1668
Alw26I	GTCTC	4 cut(s)	87, 298, 2204, 2383
AlwI	GGATC	7 cut(s)	692, 1971, 2131, 2262, 2275, 2372, 2385
AlwNI	CAGNNNCTG	1 cut(s)	2126
AoxI	GGCC	3 cut(s)	134, 157, 301
ApeKI	GCWGC	4 cut(s)	656, 944, 966, 1403
ApoI	RAATTY	4 cut(s)	1050, 1276, 1381, 1782
Asp700I	GAANNNNTTC	1 cut(s)	1259
AspLEI	GCGC	2 cut(s)	829, 2291
AspS9I	GGNCC	5 cut(s)	566, 623, 1146, 1332, 1457
AsuHPI	GGTGA	9 cut(s)	89, 620, 1228, 1324, 1426, 1685, 1853, 2132, 2201
AsuII	TTCGAA	1 cut(s)	1263
AvaII	GGWCC	5 cut(s)	566, 623, 1146, 1332, 1457
AxyI	CCTNAGG	3 cut(s)	561, 1863, 1959
BamHI	GGATCC	2 cut(s)	2267, 2377
BanI	GGYRCC	1 cut(s)	2288
BanII	GRGCYC	1 cut(s)	1307
BauI	CACGAG	2 cut(s)	254, 416
BbrPI	CACGTG	1 cut(s)	19
BbsI	GAAGAC	2 cut(s)	521, 2229
Bbv12I	GWGCWC	1 cut(s)	1668
BbvI	GCAGC	4 cut(s)	668, 953, 956, 1415
BccI	CCATC	3 cut(s)	149, 598, 2293
BceAI	ACGGC	3 cut(s)	49, 974, 2397
BciT130I	CCWGG	3 cut(s)	170, 1562, 2374
BciVI	GTATCC	1 cut(s)	1314
BclI	TGATCA	2 cut(s)	1000, 2071
BcoDI	GTCTC	4 cut(s)	87, 298, 2204, 2383
BfaI	CTAG	4 cut(s)	107, 627, 929, 2216
BfmI	CTRYAG	1 cut(s)	942
BfoI	RGCGCY	1 cut(s)	2292
BfrI	CTTAAG	2 cut(s)	422, 524
BfuI	GTATCC	1 cut(s)	1314
BglII	AGATCT	3 cut(s)	887, 1438, 1884
BisI	GCNGC	5 cut(s)	288, 657, 945, 967, 1404
BlsI	GCNGC	5 cut(s)	289, 658, 946, 968, 1405
BmcAI	AGTACT	3 cut(s)	510, 2320, 2365
Bme1390I	CCNGG	3 cut(s)	170, 1562, 2374
Bme18I	GGWCC	5 cut(s)	566, 623, 1146, 1332, 1457
BmgBI	CACGTC	1 cut(s)	2255
BmgT120I	GGNCC	5 cut(s)	566, 623, 1146, 1332, 1457
BmiI	GGNNCC	6 cut(s)	228, 567, 1459, 2269, 2290, 2379
BmrFI	CCNGG	3 cut(s)	170, 1562, 2374
BmsI	GCATC	2 cut(s)	240, 1398
BpiI	GAAGAC	2 cut(s)	521, 2229
Bpu10I	CCTNAGC	1 cut(s)	1947
Bpu14I	TTCGAA	1 cut(s)	1263
BpuEI	CTTGAG	2 cut(s)	807, 877
BsaAI	YACGTR	1 cut(s)	19
BsaHI	GRCGYC	2 cut(s)	1909, 2289
BsaJI	CCNNGG	7 cut(s)	73, 1087, 1669, 2292, 2372, 2373, 2413
Bsc4I	CCNNNNNNNGG	4 cut(s)	237, 464, 1834, 2120
Bse1I	ACTGG	4 cut(s)	657, 1416, 1484, 2163
Bse21I	CCTNAGG	3 cut(s)	561, 1863, 1959
Bse3DI	GCAATG	6 cut(s)	213, 219, 518, 1290, 1335, 1731
BseBI	CCWGG	3 cut(s)	170, 1562, 2374
BseDI	CCNNGG	7 cut(s)	73, 1087, 1669, 2292, 2372, 2373, 2413
BseGI	GGATG	4 cut(s)	255, 1216, 1429, 2304
BseLI	CCNNNNNNNGG	4 cut(s)	237, 464, 1834, 2120
BseMI	GCAATG	6 cut(s)	213, 219, 518, 1290, 1335, 1731
BseMII	CTCAG	6 cut(s)	309, 552, 1761, 1854, 1973, 2042
BseNI	ACTGG	4 cut(s)	657, 1416, 1484, 2163
BseRI	GAGGAG	2 cut(s)	779, 2000
BseXI	GCAGC	4 cut(s)	668, 953, 956, 1415
BseYI	CCCAGC	1 cut(s)	1097
Bsh1285I	CGRYCG	2 cut(s)	49, 839
BshFI	GGCC	3 cut(s)	136, 159, 303
BshNI	GGYRCC	1 cut(s)	2288
BsiEI	CGRYCG	2 cut(s)	49, 839
BsiHKAI	GWGCWC	1 cut(s)	1668
BsiSI	CCGG	2 cut(s)	160, 2381
BslFI	GGGAC	2 cut(s)	1443, 1823
BslI	CCNNNNNNNGG	4 cut(s)	237, 464, 1834, 2120
BsmAI	GTCTC	4 cut(s)	87, 298, 2204, 2383
BsmFI	GGGAC	2 cut(s)	1443, 1823
BsmI	GAATGC	1 cut(s)	274
BsnI	GGCC	3 cut(s)	136, 159, 303
Bsp119I	TTCGAA	1 cut(s)	1263
Bsp1286I	GDGCHC	2 cut(s)	1307, 1668
Bsp1407I	TGTACA	2 cut(s)	189, 1516
Bsp19I	CCATGG	1 cut(s)	73
BspACI	CCGC	6 cut(s)	45, 70, 224, 272, 287, 1500
BspANI	GGCC	3 cut(s)	136, 159, 303
BspCNI	CTCAG	6 cut(s)	308, 553, 1762, 1855, 1972, 2041
BspHI	TCATGA	1 cut(s)	1657
BspLI	GGNNCC	6 cut(s)	228, 567, 1459, 2269, 2290, 2379
BspMAI	CTGCAG	1 cut(s)	946
BspPI	GGATC	7 cut(s)	692, 1971, 2131, 2262, 2275, 2372, 2385
BspQI	GCTCTTC	2 cut(s)	845, 1656
BspT104I	TTCGAA	1 cut(s)	1263
BspT107I	GGYRCC	1 cut(s)	2288
BspTI	CTTAAG	2 cut(s)	422, 524
BsrDI	GCAATG	6 cut(s)	213, 219, 518, 1290, 1335, 1731
BsrGI	TGTACA	2 cut(s)	189, 1516
BsrI	ACTGG	4 cut(s)	657, 1416, 1484, 2163
BssECI	CCNNGG	7 cut(s)	73, 1087, 1669, 2292, 2372, 2373, 2413
BssNI	GRCGYC	2 cut(s)	1909, 2289
BssSI	CACGAG	2 cut(s)	254, 416
BssT1I	CCWWGG	2 cut(s)	73, 2292
Bst2BI	CACGAG	2 cut(s)	254, 416
Bst2UI	CCWGG	3 cut(s)	170, 1562, 2374
Bst4CI	ACNGT	7 cut(s)	790, 840, 1521, 1653, 1670, 1822, 2144
Bst6I	CTCTTC	3 cut(s)	845, 1568, 1656
BstACI	GRCGYC	2 cut(s)	1909, 2289
BstAFI	CTTAAG	2 cut(s)	422, 524
BstAPI	GCANNNNNTGC	1 cut(s)	986
BstAUI	TGTACA	2 cut(s)	189, 1516
BstBAI	YACGTR	1 cut(s)	19
BstBI	TTCGAA	1 cut(s)	1263
BstC8I	GCNNGC	7 cut(s)	239, 734, 1297, 1599, 1636, 2057, 2105
BstDSI	CCRYGG	4 cut(s)	73, 1087, 1669, 2413
BstENI	CCTNNNNNAGG	2 cut(s)	1832, 2118
BstF5I	GGATG	4 cut(s)	255, 1216, 1429, 2304
BstH2I	RGCGCY	1 cut(s)	2292
BstHHI	GCGC	2 cut(s)	829, 2291
BstMAI	GTCTC	4 cut(s)	87, 298, 2204, 2383
BstMCI	CGRYCG	2 cut(s)	49, 839
BstMWI	GCNNNNNNNGC	5 cut(s)	42, 653, 662, 826, 986
BstNI	CCWGG	3 cut(s)	170, 1562, 2374
BstNSI	RCATGY	3 cut(s)	1243, 1638, 1898
BstSCI	CCNGG	3 cut(s)	168, 1560, 2372
BstSFI	CTRYAG	1 cut(s)	942
BstV1I	GCAGC	4 cut(s)	668, 953, 956, 1415
BstV2I	GAAGAC	2 cut(s)	521, 2229
BstX2I	RGATCY	6 cut(s)	887, 1438, 1884, 2123, 2267, 2377
BstXI	CCANNNNNNTGG	1 cut(s)	2299
BstYI	RGATCY	6 cut(s)	887, 1438, 1884, 2123, 2267, 2377
Bsu36I	CCTNAGG	3 cut(s)	561, 1863, 1959
BsuI	GTATCC	1 cut(s)	1314
BsuRI	GGCC	3 cut(s)	136, 159, 303
BtgI	CCRYGG	4 cut(s)	73, 1087, 1669, 2413
BtrI	CACGTC	1 cut(s)	2255
BtsCI	GGATG	4 cut(s)	255, 1216, 1429, 2304
BtsIMutI	CAGTG	3 cut(s)	1010, 1409, 1526
Cac8I	GCNNGC	7 cut(s)	239, 734, 1297, 1599, 1636, 2057, 2105
CaiI	CAGNNNCTG	1 cut(s)	2126
CciI	TCATGA	1 cut(s)	1657
CfoI	GCGC	2 cut(s)	829, 2291
Cfr13I	GGNCC	5 cut(s)	566, 623, 1146, 1332, 1457
CseI	GACGC	1 cut(s)	1913
Csp6I	GTAC	8 cut(s)	190, 509, 902, 1517, 1990, 2038, 2319, 2364
CviQI	GTAC	8 cut(s)	190, 509, 902, 1517, 1990, 2038, 2319, 2364
DinI	GGCGCC	1 cut(s)	2290
DraI	TTTAAA	1 cut(s)	2404
DrdI	GACNNNNNNGTC	1 cut(s)	2281
DseDI	GACNNNNNNGTC	1 cut(s)	2281
EaeI	YGGCCR	1 cut(s)	157
Eam1104I	CTCTTC	3 cut(s)	845, 1568, 1656
EarI	CTCTTC	3 cut(s)	845, 1568, 1656
EciI	GGCGGA	2 cut(s)	59, 1515
Eco130I	CCWWGG	2 cut(s)	73, 2292
Eco24I	GRGCYC	1 cut(s)	1307
Eco32I	GATATC	1 cut(s)	1376
Eco47I	GGWCC	5 cut(s)	566, 623, 1146, 1332, 1457
Eco57I	CTGAAG	4 cut(s)	533, 687, 1548, 2228
Eco72I	CACGTG	1 cut(s)	19
Eco81I	CCTNAGG	3 cut(s)	561, 1863, 1959
EcoNI	CCTNNNNNAGG	2 cut(s)	1832, 2118
EcoO109I	RGGNCCY	1 cut(s)	566
EcoRII	CCWGG	3 cut(s)	168, 1560, 2372
EcoRV	GATATC	1 cut(s)	1376
EcoT14I	CCWWGG	2 cut(s)	73, 2292
EcoT22I	ATGCAT	1 cut(s)	688
EcoT38I	GRGCYC	1 cut(s)	1307
EgeI	GGCGCC	1 cut(s)	2290
EheI	GGCGCC	1 cut(s)	2290
ErhI	CCWWGG	2 cut(s)	73, 2292
FalI	AAGNNNNNCTT	2 cut(s)	518, 550
FaqI	GGGAC	2 cut(s)	1443, 1823
FauI	CCCGC	1 cut(s)	279
FbaI	TGATCA	2 cut(s)	1000, 2071
FblI	GTMKAC	2 cut(s)	1954, 2018
Fnu4HI	GCNGC	5 cut(s)	288, 657, 945, 967, 1404
FokI	GGATG	4 cut(s)	262, 1223, 1436, 2311
FriOI	GRGCYC	1 cut(s)	1307
Fsp4HI	GCNGC	5 cut(s)	288, 657, 945, 967, 1404
FspBI	CTAG	4 cut(s)	107, 627, 929, 2216
GlaI	GCGC	2 cut(s)	828, 2290
GluI	GCNGC	5 cut(s)	288, 657, 945, 967, 1404
GsaI	CCCAGC	1 cut(s)	1101
HaeII	RGCGCY	1 cut(s)	2292
HaeIII	GGCC	3 cut(s)	136, 159, 303
HapII	CCGG	2 cut(s)	160, 2381
HgaI	GACGC	1 cut(s)	1913
HhaI	GCGC	2 cut(s)	829, 2291
Hin1I	GRCGYC	2 cut(s)	1909, 2289
Hin6I	GCGC	2 cut(s)	827, 2289
HinP1I	GCGC	2 cut(s)	827, 2289
HincII	GTYRAC	3 cut(s)	793, 1032, 2019
HindII	GTYRAC	3 cut(s)	793, 1032, 2019
HindIII	AAGCTT	4 cut(s)	198, 1297, 1791, 2101
HinfI	GANTC	9 cut(s)	430, 446, 545, 740, 1083, 1154, 1315, 1615, 2117
HpaI	GTTAAC	1 cut(s)	793
HpaII	CCGG	2 cut(s)	160, 2381
HphI	GGTGA	9 cut(s)	89, 620, 1228, 1324, 1426, 1685, 1853, 2132, 2201
Hpy166II	GTNNAC	7 cut(s)	501, 793, 1032, 1816, 1955, 2019, 2305
Hpy8I	GTNNAC	7 cut(s)	501, 793, 1032, 1816, 1955, 2019, 2305
Hpy99I	CGWCG	1 cut(s)	2259
HpyAV	CCTTC	2 cut(s)	711, 2252
HpyCH4III	ACNGT	7 cut(s)	790, 840, 1521, 1653, 1670, 1822, 2144
HpyCH4IV	ACGT	6 cut(s)	18, 727, 1812, 1909, 1933, 2254
HpyF10VI	GCNNNNNNNGC	5 cut(s)	42, 653, 662, 826, 986
HpySE526I	ACGT	6 cut(s)	18, 727, 1812, 1909, 1933, 2254
Hsp92I	GRCGYC	2 cut(s)	1909, 2289
HspAI	GCGC	2 cut(s)	827, 2289
KasI	GGCGCC	1 cut(s)	2288
Ksp22I	TGATCA	2 cut(s)	1000, 2071
KspAI	GTTAAC	1 cut(s)	793
LguI	GCTCTTC	2 cut(s)	845, 1656
LmnI	GCTCC	2 cut(s)	360, 617
Lsp1109I	GCAGC	4 cut(s)	668, 953, 956, 1415
LweI	GCATC	2 cut(s)	240, 1398
MaeI	CTAG	4 cut(s)	107, 627, 929, 2216
MaeII	ACGT	6 cut(s)	18, 727, 1812, 1909, 1933, 2254
MaeIII	GTNAC	1 cut(s)	1108
MfeI	CAATTG	1 cut(s)	1398
MflI	RGATCY	6 cut(s)	887, 1438, 1884, 2123, 2267, 2377
MhlI	GDGCHC	2 cut(s)	1307, 1668
Mly113I	GGCGCC	1 cut(s)	2289
MlyI	GAGTC	3 cut(s)	455, 1092, 1324
MmeI	TCCRAC	3 cut(s)	741, 1298, 1464
Mph1103I	ATGCAT	1 cut(s)	688
MroXI	GAANNNNTTC	1 cut(s)	1259
MslI	CAYNNNNRTG	6 cut(s)	17, 576, 602, 1412, 1671, 2340
MspA1I	CMGCKG	2 cut(s)	45, 659
MspCI	CTTAAG	2 cut(s)	422, 524
MspI	CCGG	2 cut(s)	160, 2381
MspR9I	CCNGG	3 cut(s)	170, 1562, 2374
MssI	GTTTAAAC	1 cut(s)	2404
MunI	CAATTG	1 cut(s)	1398
Mva1269I	GAATGC	1 cut(s)	274
MvaI	CCWGG	3 cut(s)	170, 1562, 2374
MwoI	GCNNNNNNNGC	5 cut(s)	42, 653, 662, 826, 986
NarI	GGCGCC	1 cut(s)	2289
NcoI	CCATGG	1 cut(s)	73
NlaIV	GGNNCC	6 cut(s)	228, 567, 1459, 2269, 2290, 2379
NmeAIII	GCCGAG	1 cut(s)	161
NmuCI	GTSAC	1 cut(s)	1108
NsiI	ATGCAT	1 cut(s)	688
NspI	RCATGY	3 cut(s)	1243, 1638, 1898
NspV	TTCGAA	1 cut(s)	1263
OliI	CACNNNNGTG	3 cut(s)	17, 1412, 1671
PaeI	GCATGC	1 cut(s)	1638
PagI	TCATGA	1 cut(s)	1657
PasI	CCCWGGG	1 cut(s)	2373
PciSI	GCTCTTC	2 cut(s)	845, 1656
PcsI	WCGNNNNNNNCGW	2 cut(s)	24, 1818
PctI	GAATGC	1 cut(s)	274
PdmI	GAANNNNTTC	1 cut(s)	1259
PfeI	GAWTC	6 cut(s)	430, 545, 740, 1154, 1615, 2117
PflFI	GACNNNGTC	1 cut(s)	65
PfoI	TCCNGGA	2 cut(s)	168, 1560
PkrI	GCNGC	5 cut(s)	289, 658, 946, 968, 1405
PleI	GAGTC	3 cut(s)	454, 1091, 1323
PluTI	GGCGCC	1 cut(s)	2292
PmaCI	CACGTG	1 cut(s)	19
PmeI	GTTTAAAC	1 cut(s)	2404
PmlI	CACGTG	1 cut(s)	19
PpsI	GAGTC	3 cut(s)	454, 1091, 1323
Ppu21I	YACGTR	1 cut(s)	19
PpuMI	RGGWCCY	1 cut(s)	566
Psp1406I	AACGTT	1 cut(s)	727
Psp5II	RGGWCCY	1 cut(s)	566
Psp6I	CCWGG	3 cut(s)	168, 1560, 2372
PspCI	CACGTG	1 cut(s)	19
PspFI	CCCAGC	1 cut(s)	1097
PspGI	CCWGG	3 cut(s)	168, 1560, 2372
PspN4I	GGNNCC	6 cut(s)	228, 567, 1459, 2269, 2290, 2379
PspPI	GGNCC	5 cut(s)	566, 623, 1146, 1332, 1457
PspPPI	RGGWCCY	1 cut(s)	566
PsrI	GAACNNNNNNTAC	2 cut(s)	1982, 2014
PstI	CTGCAG	1 cut(s)	946
PstNI	CAGNNNCTG	1 cut(s)	2126
PsuI	RGATCY	6 cut(s)	887, 1438, 1884, 2123, 2267, 2377
PsyI	GACNNNGTC	1 cut(s)	65
PvuII	CAGCTG	1 cut(s)	659
RsaI	GTAC	8 cut(s)	191, 510, 903, 1518, 1991, 2039, 2320, 2365
RsaNI	GTAC	8 cut(s)	190, 509, 902, 1517, 1990, 2038, 2319, 2364
RseI	CAYNNNNRTG	6 cut(s)	17, 576, 602, 1412, 1671, 2340
SalI	GTCGAC	1 cut(s)	2017
SapI	GCTCTTC	2 cut(s)	845, 1656
SatI	GCNGC	5 cut(s)	288, 657, 945, 967, 1404
Sau96I	GGNCC	5 cut(s)	566, 623, 1146, 1332, 1457
ScaI	AGTACT	3 cut(s)	510, 2320, 2365
SchI	GAGTC	3 cut(s)	455, 1092, 1324
ScrFI	CCNGG	3 cut(s)	170, 1562, 2374
SduI	GDGCHC	2 cut(s)	1307, 1668
SfaNI	GCATC	2 cut(s)	240, 1398
SfcI	CTRYAG	1 cut(s)	942
SfoI	GGCGCC	1 cut(s)	2290
SfuI	TTCGAA	1 cut(s)	1263
SinI	GGWCC	5 cut(s)	566, 623, 1146, 1332, 1457
SmiMI	CAYNNNNRTG	6 cut(s)	17, 576, 602, 1412, 1671, 2340
SmlI	CTYRAG	4 cut(s)	422, 524, 822, 856
SmoI	CTYRAG	4 cut(s)	422, 524, 822, 856
SphI	GCATGC	1 cut(s)	1638
SsiI	CCGC	6 cut(s)	45, 70, 224, 272, 287, 1500
SspDI	GGCGCC	1 cut(s)	2288
SspI	AATATT	1 cut(s)	1568
SspMI	CTAG	4 cut(s)	107, 627, 929, 2216
StyD4I	CCNGG	3 cut(s)	168, 1560, 2372
StyI	CCWWGG	2 cut(s)	73, 2292
TaaI	ACNGT	7 cut(s)	790, 840, 1521, 1653, 1670, 1822, 2144
TaiI	ACGT	6 cut(s)	21, 730, 1815, 1912, 1936, 2257
TaqI	TCGA	4 cut(s)	444, 952, 1263, 2018
TatI	WGTACW	8 cut(s)	189, 508, 901, 1516, 1989, 2037, 2318, 2363
TauI	GCSGC	1 cut(s)	290
TfiI	GAWTC	6 cut(s)	430, 545, 740, 1154, 1615, 2117
TscAI	CASTG	3 cut(s)	1010, 1416, 1526
TseFI	GTSAC	1 cut(s)	1108
TseI	GCWGC	4 cut(s)	656, 944, 966, 1403
Tsp45I	GTSAC	1 cut(s)	1108
TspDTI	ATGAA	8 cut(s)	367, 594, 657, 1485, 1628, 1646, 1674, 1742
TspGWI	ACGGA	2 cut(s)	985, 1076
TspRI	CASTG	3 cut(s)	1010, 1416, 1526
Tth111I	GACNNNGTC	1 cut(s)	65
Vha464I	CTTAAG	2 cut(s)	422, 524
VpaK11BI	GGWCC	5 cut(s)	566, 623, 1146, 1332, 1457
XagI	CCTNNNNNAGG	2 cut(s)	1832, 2118
XapI	RAATTY	4 cut(s)	1050, 1276, 1381, 1782
XceI	RCATGY	3 cut(s)	1243, 1638, 1898
XmiI	GTMKAC	2 cut(s)	1954, 2018
XmnI	GAANNNNTTC	1 cut(s)	1259
XspI	CTAG	4 cut(s)	107, 627, 929, 2216
ZraI	GACGTC	1 cut(s)	1910
ZrmI	AGTACT	3 cut(s)	510, 2320, 2365
Zsp2I	ATGCAT	1 cut(s)	688

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.