RchiOBHm_Chr1g0380531

gamma-irradiation and mitomycin c induced

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0001133
Physical Location & Seq
Forward (+)
66186690 .. 66200740
14051 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4773 bp
ATGGATAAGGAATATAATTTCCAGATCCTATTACCAAATGGCACAAGCGTCCGTTTGAAATTGCAAAATCCTGAGCCAAAAATGCCATTCAGAGATTTCATTCAAAGGGTGGAAAAGGAATACATCCGCACTTGGAAACAATCAGGCTCACTGAAGCGGAAACGGGAGATTAACTGGAAAGGTGGTAGCTTTCTTCTTGTAGATGCTGATGATATGAAGATACAAAATGCAGTGAATTTCAAGAACTTCAACACACAAAAGTGTCACATTCTGAGGCTTCAAGATGGTCTGCGGGAGAGCGCTACTACTTTTGAGAACATGTGGGATCTGACACCTGATACTGAGATACTGAAGGAACTACCGCAAGAGTATACATTTGAGACTGCTCTGGCAGATTTGATTGATAATTCTCTGCAAGCTGTATGGTCAAATGAAAGGAGAGATGTTAAACATATAAGTGTGAATGTTGATGATGATATGATCTCAATTTTTGACACTGGCCCAGGGATGGATGGAAGTGACGAAAACTCTATAGTGAAGTGGGGAAAGATGGGTGCTTCACTTCACCGATCGTATAAGGAACAGGCAATAGGGGGTAAACCCCCGTACTTAAAGCCGTATTTTGGAATGTTTGGATATGGTGGACCTATAGCCTCCATGGAGTTGGGGAGGCGTGCATTAGTTTCTTCTAAGACAAAGGATTCAAAGAAAGTTTATACCTTGAATCTTGATAGAGATGCTTTACTCAGTCGGTCTGATTGGAACACTGGTGGTAGCATGAGGGATCCTCAAGAGGATGAGATAAGCAGAACACCGCACAGAAGCTTTACTAAGGTTGAAATCTTTGAACCTAAATCAAAATTGGACACATCTGAACTCCGTTGCAAGCTGAAGGATATATATTTTCCATATATTCAGTATGATGAAGATACAAAATCAGGGAAGACCATAATGCCAGTCAAGTTTGAGGTCAATGGCGTTGATTTGGCTGAGATTCAGGGCGGTGAAATTGCAATTACCAACTTGCATTCCTGCAATGGTCCTGATTTTGTTTTACAGCTTCAGTTCTCCTGGAAGCAAGATAATATGAGAAAATGTAAATCTCCAGATTCAAAATATACCAAAGCTAATGCTCGCCTGAAATGCGTTTATTTCCCTATTGTTAAGGGAAAAGAGAACATTGACAGAATCCTGGAGGAATGCAGGATCACAGAAAATTTTGAGACCTTTAGTCGTGTCTCTATTCGGAGGCTTGGCCGCCTGCTTCCTGATGCTCGTTGGGGTTTGCTACCTTTCATGGATCTCAAGCAAAAAAGAGGAGATATGGCACAATTGTTGAAAAGATGTTGCATGAGAGTTAAATGCTTTATAGAGACTGATGCTGGTTTCAATCCAACATCATCGAAGACTGATCTAGCACACCATAGTCCTGTCACAACCGCATTGAGGAATTTCGGCAATAAGCCTCTTGAGAATGAGAATGACATCAAAGTTCAAATTTATAAGGATGGAAATCTGCTAAATCTTTCTCAACTAAAGAAGGACTATGAGGATTGGATAGTTCAGATGCATGCTCGATATGATGATGATGAAGCTGACTGTGGTGAAGACCAACCTGTCTTCGTTGTCAATCCTGCAAACAAAAAGGCACTTCGTATTTCATCTGAAGTTGCAAGGTTGCATAAATCTATAACGAGGAATGGAAGGACCTGGAAGCGTGGTCAAAGAATTAAAATTTTTAAGGGAGCATGTGTTGGGGTTCAAAAAAACAATGTTTATGCCACAATTGAATACTTTTTGCTTGAAGGTCTCCAAGATGAATCTGGTGGTGAGGCTCGAATTATATGCAGGCCTTCAAGTCTGTCAGATGAAAAGGGTTGCTTCCTTTCAGTCAATGATGGCGATACTAATTTGGATATGGGTGAATCCTTGTCTATACCACTCAGTGTGATTGATGCTGGAAAGTGCATAGCTGTTGAAAGAACCGAGTGGGAAAACCATATAGAGAGACGACGCCAAAGATCTCCTTCTACAATTGAACTTCTTGATGCAGAACAATGTCAAGAGTTGGAGGTTGATGGGGCATTACCTGTGGATGCTCGAGCTGGAAAAGCTCCTCCAGAAGAAATTGTGGCCATTGTACGACCCGGCAATTATGTCTGTTCCAGTTCTTCAAAAAGCCTGGATCAGAAGTGCATCGTCAAAAGTAATTTAGAAATGTCTATGGAAGTTAACTTCAGAGACGATGCTGAAGAACTTCAAAATGTTCGTCATATTTTTTCGGTGTGCGTAGGACCCAAGTCACTTAAGGGAATTCAGGGGTTATATGTTTTTCCAGTTAAACGCAAGCTGCCTGGTTTCTTTCGAAGTGCTGGTATCTATACATTCTCATTTCGTCTTAATGAGTCAGATTGTAAGAGTGCTGAGAGAAAAGTACGAATCAAGCCATCTTCCAAGGTCGGAAAGTGGGTCCTTCTAAGCGGTGATCAGCCTCCACTGTACCGAGTGAGAGCTGGTTCAGTATTTTCACCTCTCTCTATAGCATGTTACGATGTATATGACAATAGAATTCCATTTCCAACTACTCTGGAAGTCACGGTGAAAGTTCAGACAAATGAGGGTGTGCTTTTCCATGTAGAGAAATTCAAAAAAGAACTTTCAACCCATACTTTGACAGTAAAGGATATGCTGATGGAGAGCAGTGAGTTAGACAAACTTCGTCCTACATATGGAGCTACTTTATTTGTATGTACAGAAGATGACAAATTTTCAGTTTCGGTTCCATGTGAAGTTAATCCTGGACCTCTACAGATTGTTAAGGCTATTCCTCCTATTTTTGAAAACCAAGTGCTTCCAGATTATACTATTCAGGAGTTTATTTTAGAGATGTTTGATCAATATGGCAATCATGTAATAGAAGGCACTGAAGTTCAATTGAATGTGGAGGGATTTTCTATTCAAGATCGACTAGGTACAGCACGTACGGCTGATCATCATGGATGCATTGACCTTGGTGGCCTCTTGAAAGTAACCGCAGGTTATGGGAAAAGTGTATCAATTTCTGTCTACTCCAACAATGATGTTTTACTGAATCTTCAGTCTCAAACTGAGAAAAGGGTGTTGAGAATTTCATCAAAGGTGCCTGACGTTTGTGTGGCTGGAACTCAAATGGAAAATATGGTTTTTGAAATTGTCAACTCTGATGGTGTTGTTGATGATACTATTCATCATGACGAGAAAAGTGGCCAATTACATATGCTTACAATAAAGGCCGGTTCATCATTCATGGAGGAATCCCTTCGATACACATTCAAGCATGGCCGCTGTACTGTTCCGGCTATTTTTATTCCTGAAGTTGAAGAGAGTTTTAACTTTGTAGCTGCTCATTCATCTCATCCAGAACTGCATTTGAATGTGAAGGTTCCTGTAGTACAAGCTCCAAAAGAGAAATATGATCATTCTCTCATAAAAACAGAGAAGCATGATGAGACTCCAATTCCGTGGGAGGTTTCTCCACTCCGAGAGTCACCACTCCGAGAGTCACAGCAGCAACTAGGAAACTTAATGGTTCCAAAAGTGGAGCATCAAGAGTTTCAATCCCCATGTACTAATGGAAACACATCGCTTCTTCCAGATTCTTCTTGCCTTCTGCAATTAGAAAACTTTAAGGAACTGCAGAATGTCATCCACAAACATGGAATACGCATTGGAGATATGGAAGAAAAATTGGACCTTCTAAAGCAAGAGAAGGCTAAAGTGAAGAAAGAGCTCATTGATTTGCAAGGGTCTATTGAACCTCACAAAGAGGAATCGATAAACCGCATAGAAAGGATGAGTAACACTGCAGCTGCTTTTGTCTGTACTCTATCTAGGGAAGTTTCATTTGAAGAGGTAAACCATCATTTCATGGGAGATGTGATTGGTTTGGTTGCCCTACTTGGTACAGTTGGTACTAGTAATCTAAGCAGGATATTGGCTGAATACTTGGGTAAAGATCAGATGCTTGCTATTGTCTGTAGATCCTTTGAAGCAGCTGGAGCTCTTGAGAAGTTTGTACAGAGTGATTATGGGGAAGTTGATTCTATGCATGCGCTCTGTGAACCAGCTCTACTTCAGAGACCTACACATGGTCGATCTTTTGTTATATGTCTGGAAGACATAAGGCCTTACACTGGTGATATCAACGGCAGTGACCCTCAAAAGAAACTGGCATTGCCAGATCCTGTCTTGCCAGGTGGAATGGCTCCTGATGGTTTCTTAGGATATGCAGTCAATATGGTTGATCTGGATAGCCATCATTTACAGACGGTGACATCTGCAGGCCATGGTCTCAGGGAGACAGTATTATACTGTCTTTTCGGCGAGCTCCAAGTGTATAAAACCAGGGAAGATATGTTGGCTGCTCGTGCTTGTATTAAGCATGGTGCTATTTCTCTGGATGGAGGAATTTTGAAACAGAGTGGAGTAGTCTCTTTTGGAATCGGGGAGCCAGAAATATGTTTTCCGGTAGCAGAAAGTCTGGCACTTTCGACATCAACAAATTTTGTTGAAGTTGAGCAGCTGATCAAGGAGAAGAAGTCAAAGTTGACGATGATCAATTGTTGTATGGAAAAAGTGACCAAAATCCATCGGAGATCATTAAGGAAATTCAAGAAGTCAAAGAAGAAGGGGCACAAGCTGTTGGAGAACATTCGCTCTACCCAAAAAGATTCTCCAAGTCCGATGAAAGAAGGGAGAGAGCTCGTAAAAGCTAGCAGCTCTCAAAGTTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HATPase_c_3

Protein Analysis

1590

Amino Acids

179.0

Weight (kDa)

6.41

Isoelectric Point (pI)

44.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HATPase_c_3 PF13589 124 - 255 1e-10 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1503
AasI GACNNNNNNGTC 1 cut(s) 1616
Acc36I ACCTGC 1 cut(s) 3026
AccB1I GGYRCC 1 cut(s) 3139
AccI GTMKAC 2 cut(s) 371, 3066
AclWI GGATC 9 cut(s) 19, 333, 779, 792, 1214, 1308, 2193, 4014, 4214
AcoI YGGCCR 4 cut(s) 1255, 2133, 3244, 3319
AcyI GRCGYC 1 cut(s) 2014
AdeI CACNNNGTG 1 cut(s) 1946
AfeI AGCGCT 1 cut(s) 301
AfiI CCNNNNNNNGG 6 cut(s) 508, 623, 1446, 2729, 4127, 4172
AflII CTTAAG 1 cut(s) 2306
AflIII ACRYGT 1 cut(s) 318
AhdI GACNNNNNGTC 1 cut(s) 1230
AhlI ACTAGT 1 cut(s) 3953
AjnI CCWGG 9 cut(s) 502, 1070, 1191, 1709, 2181, 2353, 2797, 4231, 4382
AleI CACNNNNGTG 1 cut(s) 259
Alw21I GWGCWC 4 cut(s) 3771, 4042, 4368, 4743
AlwI GGATC 9 cut(s) 19, 333, 779, 792, 1214, 1308, 2193, 4014, 4214
AlwNI CAGNNNCTG 1 cut(s) 4223
Ama87I CYCGRG 1 cut(s) 2100
Aor51HI AGCGCT 1 cut(s) 301
ApeKI GCWGC 9 cut(s) 2350, 3380, 3547, 3845, 3848, 4031, 4400, 4558, 4755
ArsI GACNNNNNNTTYG 2 cut(s) 2254, 2286
Asp700I GAANNNNTTC 2 cut(s) 2256, 3297
AspLEI GCGC 2 cut(s) 302, 4093
AspS9I GGNCC 8 cut(s) 500, 644, 1040, 1707, 2294, 2470, 2801, 3730
AsuC2I CCSGG 1 cut(s) 2148
AsuII TTCGAA 1 cut(s) 2365
AsuNHI GCTAGC 1 cut(s) 4751
AvaI CYCGRG 1 cut(s) 2100
AvaII GGWCC 7 cut(s) 644, 1040, 1707, 2294, 2470, 2801, 3730
BaeGI GKGCMC 1 cut(s) 4674
BalI TGGCCA 2 cut(s) 2135, 3246
BamHI GGATCC 1 cut(s) 784
BanI GGYRCC 1 cut(s) 3139
BanII GRGCYC 4 cut(s) 3771, 4042, 4368, 4743
BauI CACGAG 1 cut(s) 4404
BbsI GAAGAC 5 cut(s) 950, 1412, 1612, 1614, 4161
Bbv12I GWGCWC 4 cut(s) 3771, 4042, 4368, 4743
BbvI GCAGC 9 cut(s) 2337, 3367, 3559, 3835, 3857, 4043, 4387, 4570, 4767
BceAI ACGGC 3 cut(s) 601, 3000, 4201
BcgI CGANNNNNNTGC 2 cut(s) 1827, 1861
BciT130I CCWGG 9 cut(s) 504, 1072, 1193, 1711, 2183, 2355, 2799, 4233, 4384
BclI TGATCA 6 cut(s) 2485, 2893, 2989, 3454, 4563, 4593
BcnI CCSGG 1 cut(s) 2148
BcuI ACTAGT 1 cut(s) 3953
BfaI CTAG 6 cut(s) 1415, 2969, 3554, 3870, 3954, 4752
BfmI CTRYAG 9 cut(s) 531, 648, 2538, 2807, 3426, 3674, 3843, 4015, 4317
BfoI RGCGCY 1 cut(s) 303
BfrI CTTAAG 1 cut(s) 2306
BfuAI ACCTGC 1 cut(s) 3026
BglII AGATCT 1 cut(s) 2021
Bme18I GGWCC 7 cut(s) 644, 1040, 1707, 2294, 2470, 2801, 3730
BmeRI GACNNNNNGTC 1 cut(s) 1230
BmeT110I CYCGRG 1 cut(s) 2100
BmgT120I GGNCC 8 cut(s) 500, 644, 1040, 1707, 2294, 2470, 2801, 3730
BmiI GGNNCC 9 cut(s) 786, 2296, 2471, 2781, 3141, 3423, 3570, 4245, 4488
BmtI GCTAGC 1 cut(s) 4755
BoxI GACNNNNGTC 1 cut(s) 2299
BpiI GAAGAC 5 cut(s) 950, 1412, 1612, 1614, 4161
BplI GAGNNNNNCTC 2 cut(s) 772, 804
BpmI CTGGAG 4 cut(s) 1089, 1214, 2103, 4056
Bpu10I CCTNAGC 1 cut(s) 72
Bpu14I TTCGAA 1 cut(s) 2365
BpuEI CTTGAG 4 cut(s) 774, 1289, 1490, 4064
BpuMI CCSGG 1 cut(s) 2148
Bsa29I ATCGAT 1 cut(s) 3812
BsaAI YACGTR 1 cut(s) 2981
BsaBI GATNNNNATC 2 cut(s) 1512, 4293
BsaHI GRCGYC 1 cut(s) 2014
BsaI GGTCTC 4 cut(s) 1217, 1814, 4111, 4334
BsaJI CCNNGG 8 cut(s) 502, 503, 657, 2454, 3010, 3500, 4324, 4383
BsaWI WCCGGW 1 cut(s) 4504
BsaXI ACNNNNNCTCC 4 cut(s) 1737, 1767, 4562, 4592
Bsc4I CCNNNNNNNGG 6 cut(s) 508, 623, 1446, 2729, 4127, 4172
Bse118I RCCGGY 1 cut(s) 3272
Bse1I ACTGG 8 cut(s) 179, 502, 772, 956, 2166, 2336, 4177, 4212
Bse3DI GCAATG 2 cut(s) 1042, 4211
Bse8I GATNNNNATC 2 cut(s) 1512, 4293
BseBI CCWGG 9 cut(s) 504, 1072, 1193, 1711, 2183, 2355, 2799, 4233, 4384
BseCI ATCGAT 1 cut(s) 3812
BseDI CCNNGG 8 cut(s) 502, 503, 657, 2454, 3010, 3500, 4324, 4383
BseJI GATNNNNATC 2 cut(s) 1512, 4293
BseLI CCNNNNNNNGG 6 cut(s) 508, 623, 1446, 2729, 4127, 4172
BseMI GCAATG 2 cut(s) 1042, 4211
BseMII CTCAG 9 cut(s) 63, 263, 333, 760, 981, 1957, 2415, 3099, 4345
BseNI ACTGG 8 cut(s) 179, 502, 772, 956, 2166, 2336, 4177, 4212
BseRI GAGGAG 2 cut(s) 1332, 2106
BseSI GKGCMC 1 cut(s) 4674
BseXI GCAGC 9 cut(s) 2337, 3367, 3559, 3835, 3857, 4043, 4387, 4570, 4767
Bsh1285I CGRYCG 1 cut(s) 572
BshNI GGYRCC 1 cut(s) 3139
BshVI ATCGAT 1 cut(s) 3812
BsiEI CGRYCG 1 cut(s) 572
BsiHKAI GWGCWC 4 cut(s) 3771, 4042, 4368, 4743
BsiHKCI CYCGRG 1 cut(s) 2100
BsiSI CCGG 4 cut(s) 2148, 3273, 3335, 4505
BsiWI CGTACG 1 cut(s) 2981
BslI CCNNNNNNNGG 6 cut(s) 508, 623, 1446, 2729, 4127, 4172
BsmBI CGTCTC 2 cut(s) 2002, 2235
BsmI GAATGC 2 cut(s) 1027, 1205
Bso31I GGTCTC 4 cut(s) 1217, 1814, 4111, 4334
BsoBI CYCGRG 1 cut(s) 2100
Bsp119I TTCGAA 1 cut(s) 2365
Bsp1286I GDGCHC 5 cut(s) 3771, 4042, 4368, 4674, 4743
Bsp1407I TGTACA 2 cut(s) 2750, 4054
Bsp19I CCATGG 2 cut(s) 657, 4324
BspCNI CTCAG 9 cut(s) 64, 264, 334, 759, 982, 1956, 2416, 3100, 4344
BspDI ATCGAT 1 cut(s) 3812
BspHI TCATGA 1 cut(s) 3229
BspLI GGNNCC 9 cut(s) 786, 2296, 2471, 2781, 3141, 3423, 3570, 4245, 4488
BspMAI CTGCAG 3 cut(s) 3678, 3847, 4321
BspMI ACCTGC 1 cut(s) 3026
BspOI GCTAGC 1 cut(s) 4755
BspPI GGATC 9 cut(s) 19, 333, 779, 792, 1214, 1308, 2193, 4014, 4214
BspT104I TTCGAA 1 cut(s) 2365
BspT107I GGYRCC 1 cut(s) 3139
BspTI CTTAAG 1 cut(s) 2306
BspTNI GGTCTC 4 cut(s) 1217, 1814, 4111, 4334
BsrDI GCAATG 2 cut(s) 1042, 4211
BsrFI RCCGGY 1 cut(s) 3272
BsrGI TGTACA 2 cut(s) 2750, 4054
BsrI ACTGG 8 cut(s) 179, 502, 772, 956, 2166, 2336, 4177, 4212
BssAI RCCGGY 1 cut(s) 3272
BssECI CCNNGG 8 cut(s) 502, 503, 657, 2454, 3010, 3500, 4324, 4383
BssNAI GTATAC 1 cut(s) 372
BssNI GRCGYC 1 cut(s) 2014
BssSI CACGAG 1 cut(s) 4404
BssT1I CCWWGG 4 cut(s) 657, 2454, 3010, 4324
Bst1107I GTATAC 1 cut(s) 372
Bst2BI CACGAG 1 cut(s) 4404
Bst2UI CCWGG 9 cut(s) 504, 1072, 1193, 1711, 2183, 2355, 2799, 4233, 4384
Bst4CI ACNGT 9 cut(s) 1601, 2499, 2599, 2677, 3331, 3946, 4309, 4342, 4352
Bst6I CTCTTC 2 cut(s) 3354, 3882
BstACI GRCGYC 1 cut(s) 2014
BstAFI CTTAAG 1 cut(s) 2306
BstAUI TGTACA 2 cut(s) 2750, 4054
BstBAI YACGTR 1 cut(s) 2981
BstBI TTCGAA 1 cut(s) 2365
BstDSI CCRYGG 3 cut(s) 657, 3500, 4324
BstH2I RGCGCY 1 cut(s) 303
BstHHI GCGC 2 cut(s) 302, 4093
BstMCI CGRYCG 1 cut(s) 572
BstMWI GCNNNNNNNGC 6 cut(s) 82, 1143, 2111, 2984, 4037, 4406
BstNI CCWGG 9 cut(s) 504, 1072, 1193, 1711, 2183, 2355, 2799, 4233, 4384
BstNSI RCATGY 5 cut(s) 322, 1574, 1752, 2547, 4091
BstPAI GACNNNNGTC 1 cut(s) 2299
BstSFI CTRYAG 9 cut(s) 531, 648, 2538, 2807, 3426, 3674, 3843, 4015, 4317
BstSLI GKGCMC 1 cut(s) 4674
BstV1I GCAGC 9 cut(s) 2337, 3367, 3559, 3835, 3857, 4043, 4387, 4570, 4767
BstV2I GAAGAC 5 cut(s) 950, 1412, 1612, 1614, 4161
BstX2I RGATCY 7 cut(s) 24, 325, 784, 1300, 2021, 4019, 4219
BstXI CCANNNNNNTGG 3 cut(s) 664, 3501, 3695
BstYI RGATCY 7 cut(s) 24, 325, 784, 1300, 2021, 4019, 4219
BstZ17I GTATAC 1 cut(s) 372
Bsu15I ATCGAT 1 cut(s) 3812
BsuTUI ATCGAT 1 cut(s) 3812
BtgI CCRYGG 3 cut(s) 657, 3500, 4324
BtgZI GCGATG 1 cut(s) 3606
BtsI GCAGTG 4 cut(s) 237, 2707, 3840, 4195
BveI ACCTGC 1 cut(s) 3026
CaiI CAGNNNCTG 1 cut(s) 4223
CciI TCATGA 1 cut(s) 3229
CfoI GCGC 2 cut(s) 302, 4093
Cfr10I RCCGGY 1 cut(s) 3272
Cfr13I GGNCC 8 cut(s) 500, 644, 1040, 1707, 2294, 2470, 2801, 3730
ClaI ATCGAT 1 cut(s) 3812
CseI GACGC 2 cut(s) 37, 2022
DraIII CACNNNGTG 1 cut(s) 1946
DrdI GACNNNNNNGTC 1 cut(s) 1616
DriI GACNNNNNGTC 1 cut(s) 1230
DseDI GACNNNNNNGTC 1 cut(s) 1616
EaeI YGGCCR 4 cut(s) 1255, 2133, 3244, 3319
Eam1104I CTCTTC 2 cut(s) 3354, 3882
Eam1105I GACNNNNNGTC 1 cut(s) 1230
EarI CTCTTC 2 cut(s) 3354, 3882
Ecl136II GAGCTC 4 cut(s) 3769, 4040, 4366, 4741
Eco130I CCWWGG 4 cut(s) 657, 2454, 3010, 4324
Eco147I AGGCCT 2 cut(s) 1852, 4165
Eco24I GRGCYC 4 cut(s) 3771, 4042, 4368, 4743
Eco31I GGTCTC 4 cut(s) 1217, 1814, 4111, 4334
Eco32I GATATC 1 cut(s) 4180
Eco47I GGWCC 7 cut(s) 644, 1040, 1707, 2294, 2470, 2801, 3730
Eco47III AGCGCT 1 cut(s) 301
Eco53kI GAGCTC 4 cut(s) 3769, 4040, 4366, 4741
Eco88I CYCGRG 1 cut(s) 2100
EcoICRI GAGCTC 4 cut(s) 3769, 4040, 4366, 4741
EcoO109I RGGNCCY 3 cut(s) 1707, 2294, 2470
EcoRI GAATTC 2 cut(s) 2313, 2568
EcoRII CCWGG 9 cut(s) 502, 1070, 1191, 1709, 2181, 2353, 2797, 4231, 4382
EcoRV GATATC 1 cut(s) 4180
EcoT14I CCWWGG 4 cut(s) 657, 2454, 3010, 4324
EcoT22I ATGCAT 3 cut(s) 1572, 3005, 4089
EcoT38I GRGCYC 4 cut(s) 3771, 4042, 4368, 4743
ErhI CCWWGG 4 cut(s) 657, 2454, 3010, 4324
Esp3I CGTCTC 2 cut(s) 2002, 2235
FalI AAGNNNNNCTT 4 cut(s) 1865, 1897, 2011, 2043
FauI CCCGC 1 cut(s) 285
FauNDI CATATG 2 cut(s) 2728, 3255
FbaI TGATCA 6 cut(s) 2485, 2893, 2989, 3454, 4563, 4593
FblI GTMKAC 2 cut(s) 371, 3066
FriOI GRGCYC 4 cut(s) 3771, 4042, 4368, 4743
FspBI CTAG 6 cut(s) 1415, 2969, 3554, 3870, 3954, 4752
GlaI GCGC 2 cut(s) 301, 4092
GsuI CTGGAG 4 cut(s) 1089, 1214, 2103, 4056
HaeII RGCGCY 1 cut(s) 303
HapII CCGG 4 cut(s) 2148, 3273, 3335, 4505
HgaI GACGC 2 cut(s) 37, 2022
HhaI GCGC 2 cut(s) 302, 4093
Hin1I GRCGYC 1 cut(s) 2014
Hin6I GCGC 2 cut(s) 300, 4091
HinP1I GCGC 2 cut(s) 300, 4091
HincII GTYRAC 3 cut(s) 2233, 3196, 4587
HindII GTYRAC 3 cut(s) 2233, 3196, 4587
HindIII AAGCTT 1 cut(s) 823
HpaI GTTAAC 1 cut(s) 2233
HpaII CCGG 4 cut(s) 2148, 3273, 3335, 4505
Hpy166II GTNNAC 9 cut(s) 372, 599, 644, 2233, 3067, 3196, 3895, 4100, 4587
Hpy8I GTNNAC 9 cut(s) 372, 599, 644, 2233, 3067, 3196, 3895, 4100, 4587
Hpy99I CGWCG 1 cut(s) 2016
HpyCH4III ACNGT 9 cut(s) 1601, 2499, 2599, 2677, 3331, 3946, 4309, 4342, 4352
HpyCH4IV ACGT 2 cut(s) 2980, 3147
HpyF10VI GCNNNNNNNGC 6 cut(s) 82, 1143, 2111, 2984, 4037, 4406
HpySE526I ACGT 2 cut(s) 2980, 3147
Hsp92I GRCGYC 1 cut(s) 2014
HspAI GCGC 2 cut(s) 300, 4091
Ksp22I TGATCA 6 cut(s) 2485, 2893, 2989, 3454, 4563, 4593
KspAI GTTAAC 1 cut(s) 2233
LmnI GCTCC 9 cut(s) 1745, 2119, 2732, 3442, 3580, 4037, 4249, 4371, 4486
Lsp1109I GCAGC 9 cut(s) 2337, 3367, 3559, 3835, 3857, 4043, 4387, 4570, 4767
MaeI CTAG 6 cut(s) 1415, 2969, 3554, 3870, 3954, 4752
MaeII ACGT 2 cut(s) 2980, 3147
MfeI CAATTG 5 cut(s) 1331, 1785, 2034, 2933, 4597
MflI RGATCY 7 cut(s) 24, 325, 784, 1300, 2021, 4019, 4219
MhlI GDGCHC 5 cut(s) 3771, 4042, 4368, 4674, 4743
MlsI TGGCCA 2 cut(s) 2135, 3246
MluNI TGGCCA 2 cut(s) 2135, 3246
MlyI GAGTC 4 cut(s) 2414, 3484, 3533, 3548
MmeI TCCRAC 6 cut(s) 1418, 2049, 2440, 2603, 3096, 4662
Mox20I TGGCCA 2 cut(s) 2135, 3246
Mph1103I ATGCAT 3 cut(s) 1572, 3005, 4089
MroXI GAANNNNTTC 2 cut(s) 2256, 3297
MscI TGGCCA 2 cut(s) 2135, 3246
MslI CAYNNNNRTG 4 cut(s) 259, 456, 3411, 3693
Msp20I TGGCCA 2 cut(s) 2135, 3246
MspA1I CMGCKG 4 cut(s) 3324, 3848, 4034, 4561
MspCI CTTAAG 1 cut(s) 2306
MspI CCGG 4 cut(s) 2148, 3273, 3335, 4505
MunI CAATTG 5 cut(s) 1331, 1785, 2034, 2933, 4597
Mva1269I GAATGC 2 cut(s) 1027, 1205
MvaI CCWGG 9 cut(s) 504, 1072, 1193, 1711, 2183, 2355, 2799, 4233, 4384
MwoI GCNNNNNNNGC 6 cut(s) 82, 1143, 2111, 2984, 4037, 4406
NciI CCSGG 1 cut(s) 2148
NcoI CCATGG 2 cut(s) 657, 4324
NdeI CATATG 2 cut(s) 2728, 3255
NheI GCTAGC 1 cut(s) 4751
NlaIV GGNNCC 9 cut(s) 786, 2296, 2471, 2781, 3141, 3423, 3570, 4245, 4488
NsiI ATGCAT 3 cut(s) 1572, 3005, 4089
NspI RCATGY 5 cut(s) 322, 1574, 1752, 2547, 4091
NspV TTCGAA 1 cut(s) 2365
OliI CACNNNNGTG 1 cut(s) 259
PaeI GCATGC 2 cut(s) 1574, 4091
PaeR7I CTCGAG 1 cut(s) 2100
PagI TCATGA 1 cut(s) 3229
PasI CCCWGGG 1 cut(s) 503
PceI AGGCCT 2 cut(s) 1852, 4165
PciI ACATGT 1 cut(s) 318
PctI GAATGC 2 cut(s) 1027, 1205
PdmI GAANNNNTTC 2 cut(s) 2256, 3297
Pfl23II CGTACG 1 cut(s) 2981
PfoI TCCNGGA 3 cut(s) 1070, 1191, 2797
Ple19I CGATCG 1 cut(s) 572
PleI GAGTC 4 cut(s) 2413, 3484, 3532, 3547
PpsI GAGTC 4 cut(s) 2413, 3484, 3532, 3547
Ppu21I YACGTR 1 cut(s) 2981
PpuMI RGGWCCY 3 cut(s) 1707, 2294, 2470
PscI ACATGT 1 cut(s) 318
PshAI GACNNNNGTC 1 cut(s) 2299
PsiI TTATAA 1 cut(s) 1503
Psp124BI GAGCTC 4 cut(s) 3771, 4042, 4368, 4743
Psp5II RGGWCCY 3 cut(s) 1707, 2294, 2470
Psp6I CCWGG 9 cut(s) 502, 1070, 1191, 1709, 2181, 2353, 2797, 4231, 4382
PspGI CCWGG 9 cut(s) 502, 1070, 1191, 1709, 2181, 2353, 2797, 4231, 4382
PspLI CGTACG 1 cut(s) 2981
PspN4I GGNNCC 9 cut(s) 786, 2296, 2471, 2781, 3141, 3423, 3570, 4245, 4488
PspPI GGNCC 8 cut(s) 500, 644, 1040, 1707, 2294, 2470, 2801, 3730
PspPPI RGGWCCY 3 cut(s) 1707, 2294, 2470
PspXI VCTCGAGB 1 cut(s) 2100
PsrI GAACNNNNNNTAC 2 cut(s) 4092, 4124
PstI CTGCAG 3 cut(s) 3678, 3847, 4321
PstNI CAGNNNCTG 1 cut(s) 4223
PsuI RGATCY 7 cut(s) 24, 325, 784, 1300, 2021, 4019, 4219
PvuI CGATCG 1 cut(s) 572
PvuII CAGCTG 3 cut(s) 3848, 4034, 4561
RseI CAYNNNNRTG 4 cut(s) 259, 456, 3411, 3693
SacI GAGCTC 4 cut(s) 3771, 4042, 4368, 4743
Sau96I GGNCC 8 cut(s) 500, 644, 1040, 1707, 2294, 2470, 2801, 3730
SchI GAGTC 4 cut(s) 2414, 3484, 3533, 3548
SduI GDGCHC 5 cut(s) 3771, 4042, 4368, 4674, 4743
SfcI CTRYAG 9 cut(s) 531, 648, 2538, 2807, 3426, 3674, 3843, 4015, 4317
Sfr274I CTCGAG 1 cut(s) 2100
SfuI TTCGAA 1 cut(s) 2365
SinI GGWCC 7 cut(s) 644, 1040, 1707, 2294, 2470, 2801, 3730
SlaI CTCGAG 1 cut(s) 2100
SmiMI CAYNNNNRTG 4 cut(s) 259, 456, 3411, 3693
SmlI CTYRAG 6 cut(s) 789, 1304, 1469, 2100, 2306, 4043
SmoI CTYRAG 6 cut(s) 789, 1304, 1469, 2100, 2306, 4043
SpeI ACTAGT 1 cut(s) 3953
SphI GCATGC 2 cut(s) 1574, 4091
SseBI AGGCCT 2 cut(s) 1852, 4165
SspMI CTAG 6 cut(s) 1415, 2969, 3554, 3870, 3954, 4752
SstI GAGCTC 4 cut(s) 3771, 4042, 4368, 4743
StuI AGGCCT 2 cut(s) 1852, 4165
StyI CCWWGG 4 cut(s) 657, 2454, 3010, 4324
TaaI ACNGT 9 cut(s) 1601, 2499, 2599, 2677, 3331, 3946, 4309, 4342, 4352
TaiI ACGT 2 cut(s) 2983, 3150
TaqII GACCGA 1 cut(s) 741
TatI WGTACW 6 cut(s) 2750, 3326, 3430, 3605, 3860, 4054
TauI GCSGC 2 cut(s) 1260, 3324
TseI GCWGC 9 cut(s) 2350, 3380, 3547, 3845, 3848, 4031, 4400, 4558, 4755
TspGWI ACGGA 3 cut(s) 41, 869, 3489
Vha464I CTTAAG 1 cut(s) 2306
VpaK11BI GGWCC 7 cut(s) 644, 1040, 1707, 2294, 2470, 2801, 3730
XceI RCATGY 5 cut(s) 322, 1574, 1752, 2547, 4091
XcmI CCANNNNNNNNNTGG 1 cut(s) 1820
XhoI CTCGAG 1 cut(s) 2100
XmiI GTMKAC 2 cut(s) 371, 3066
XmnI GAANNNNTTC 2 cut(s) 2256, 3297
XspI CTAG 6 cut(s) 1415, 2969, 3554, 3870, 3954, 4752
Zsp2I ATGCAT 3 cut(s) 1572, 3005, 4089
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.