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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 113 |
| AfaI | GTAC | 1 cut(s) | 72 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 182 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 87, 119 |
| AluBI | AGCT | 1 cut(s) | 148 |
| AluI | AGCT | 1 cut(s) | 148 |
| AoxI | GGCC | 1 cut(s) | 127 |
| ApoI | RAATTY | 1 cut(s) | 113 |
| AspS9I | GGNCC | 1 cut(s) | 128 |
| BceAI | ACGGC | 1 cut(s) | 41 |
| BfaI | CTAG | 2 cut(s) | 6, 47 |
| BlpI | GCTNAGC | 1 cut(s) | 87 |
| BmgT120I | GGNCC | 1 cut(s) | 128 |
| BpmI | CTGGAG | 1 cut(s) | 170 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 87 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 143, 173 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 182 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 182 |
| BseMII | CTCAG | 1 cut(s) | 90 |
| BshFI | GGCC | 1 cut(s) | 129 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 182 |
| BsnI | GGCC | 1 cut(s) | 129 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 87 |
| BspANI | GGCC | 1 cut(s) | 129 |
| BspCNI | CTCAG | 1 cut(s) | 91 |
| BstDEI | CTNAG | 2 cut(s) | 87, 99 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 148 |
| BsuRI | GGCC | 1 cut(s) | 129 |
| Cfr13I | GGNCC | 1 cut(s) | 128 |
| Csp6I | GTAC | 1 cut(s) | 71 |
| CviAII | CATG | 1 cut(s) | 83 |
| CviJI | RGCY | 4 cut(s) | 28, 62, 129, 148 |
| CviKI_1 | RGCY | 4 cut(s) | 28, 62, 129, 148 |
| CviQI | GTAC | 1 cut(s) | 71 |
| DdeI | CTNAG | 2 cut(s) | 87, 99 |
| FaeI | CATG | 1 cut(s) | 86 |
| FaiI | YATR | 4 cut(s) | 59, 84, 119, 183 |
| FatI | CATG | 1 cut(s) | 82 |
| FspBI | CTAG | 2 cut(s) | 6, 47 |
| GsuI | CTGGAG | 1 cut(s) | 170 |
| HaeIII | GGCC | 1 cut(s) | 129 |
| Hin1II | CATG | 1 cut(s) | 86 |
| HinfI | GANTC | 1 cut(s) | 95 |
| Hpy188I | TCNGA | 2 cut(s) | 100, 177 |
| Hpy188III | TCNNGA | 2 cut(s) | 13, 47 |
| HpyAV | CCTTC | 1 cut(s) | 71 |
| HpyF3I | CTNAG | 2 cut(s) | 87, 99 |
| Hsp92II | CATG | 1 cut(s) | 86 |
| LpnPI | CCDG | 2 cut(s) | 26, 134 |
| MaeI | CTAG | 2 cut(s) | 6, 47 |
| MboII | GAAGA | 1 cut(s) | 62 |
| MluCI | AATT | 1 cut(s) | 113 |
| MmeI | TCCRAC | 1 cut(s) | 85 |
| MnlI | CCTC | 3 cut(s) | 94, 119, 145 |
| NlaIII | CATG | 1 cut(s) | 86 |
| PfeI | GAWTC | 1 cut(s) | 95 |
| PspPI | GGNCC | 1 cut(s) | 128 |
| RsaI | GTAC | 1 cut(s) | 72 |
| RsaNI | GTAC | 1 cut(s) | 71 |
| Sau96I | GGNCC | 1 cut(s) | 128 |
| SetI | ASST | 3 cut(s) | 82, 105, 150 |
| Sse9I | AATT | 1 cut(s) | 113 |
| SspMI | CTAG | 2 cut(s) | 6, 47 |
| TaqI | TCGA | 1 cut(s) | 124 |
| TasI | AATT | 1 cut(s) | 113 |
| TatI | WGTACW | 1 cut(s) | 70 |
| TfiI | GAWTC | 1 cut(s) | 95 |
| XapI | RAATTY | 1 cut(s) | 113 |
| XbaI | TCTAGA | 1 cut(s) | 46 |
| XspI | CTAG | 2 cut(s) | 6, 47 |