Rorug07G0046500

methyltransferase DDB_G0268948

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0002857
Physical Location & Seq
Forward (+)
3381165 .. 3385046
3882 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0046500.1

Sequence Viewer

Length: 3300 bp
ATGTACTCGAACTCGAAAACTCGTTCATGGCGCTCGGTGTTGTGCTCTTCCATATTCTCACTCATCATCATGATAACAGCTGAAGTTGTAGCTGGAGATTCTCTAACCACAGACATGGAAGTTCTGCTGAGCCTTAAAGCTTTTCTTCAAGAACAAAACAGAGTAAACCAAGGCCAATACTCAAAATGGAACCAGAGCATTAGCCCCTGTCATTGGCCTAGAATCAAATGCAATGGTGACAGTAGAGTTACTGGAATCGATCTCTCCGAAAGCAAAATCTCCTGTGAGTTTTTCAGAAACTTCTCTGCCTTGACACAGCTTTCGGACCTGGACTTGTCCGATAACACTCTAGGTGGAGCTATCCCGGAGGACTTGAGTCAATGCAAGAGCCTCAAGTACCTGAATTTGTCACATAACATTCTAGAAGGTGAGCTGAACTTGCAGGGGTTGAGTCAATTGGAGGTTCTTGATTTGGCTGTGAATAGGTTTTATGGGGATATGAAGATGAGCTTCCCTGGAATTTGCAAGAACTTGGTGGTGGCAAATGTTTCATCAAACAACTTTACTGGTAGGATTGACCAGTACTTTGATGAGTGCTCGAAATTGCAGTACTTGGATTTGAGCACAAACAATTTTAGTGGAGATGTGTGGGTTGGATTCACAAGGCTTAGGGAGTTTTCGGCGTCTGAGAATCACCTCAGTGGGGCTATTTTGCCTTCTATTTTCAATGGCAGTTGTAACTTGGTGATTTTGGACCTGTCACAGAACAATTTCTCTGGTCGAGTTCCAAAGGAGATTTCAAATTGCCGGAATTTGGCTGCTTTGAATCTCTGGGGGAACAAATTTACAGGGCCAATTCCATCTGAGATTGGAAGCATTTCGAGTCTGGAGATTTTGTTCTTTGGCAATAACAGTTTTTCTAGAGTCATCCCGGAAGCCCTTTTGAATTTGAGCAACTTGGTGTTCTTGGATTTGAGCAGGAACAATTTTGGAGGAAATATACAAGAGATTTTTGGGCGATTTACGCAGGTGAAGATTCTTATGCTGCACTCAAATTCATACACAGGTGGTATATATACATCTGGAATTCTCAAGCTACCTAATGTTTCTCGGTTAGATCTCAGTAACAACAATTTTACAGGTCCTTTACCGGTTGAAATTGCTGAAATGACAAGCTTGAAGTACTTGTACCTTGCCCACAATCAATTCAGTGGAACTATACCACCAGAGTATGGAAATTGGCAACAGCTCCAAGCACTGGACCTTTCTTTCAACAGCCTAACAGGGTCAATCCCGGCCACCATAGGAAAGTTGAGGTCTCTCTTATGGTTGATGCTTGCCAATAATTCTCTAACAGGTCAAATTCCAATGGAGCTAGGGAATTGTACTAGCTTGTTGTGGCTGAACCTTTCCAACAACAAGCTCAATGGAACAATCCCATCTGAGTTGAAGAATATTGGGACAAATGCAGGTCCAACATTTGAATCAAACAATGATATCAAGGATGCGATCATAGGTGGATCCGGGGAGTGCTTAGCAATAAAGAGATGGATTCCAGCAGACTACCCTCCATTCAGTTTTGTGTACACAATCCTCACAAGGAAGAGTTGTCGAAGCATGTGGGATCGGCTACTTAAAGGAAATGGCCTTTTTCCCATATGTGTTGCTGGTTCTGCTGTAAGGACATTGCAAATCTCAGGTTATGTACAACTGAGTGGGAACCAGCTTTCAGGTCAGGTCCCTCCAGAGATTGGCGAGATGCGCAATTTTAGTATGATGAATTTGGCTTTCAATCAATTAAGTGGAGAACTCCCTGCCGGTATTGGACAATTGCCACTCGTAGTGTTTAACCTCAGTAAGAACAGTTTCTCGGGCGAAATCCCAATGGAAATTGGGAACAGTAAGTGTATGCAGAATCTTGATTTGTCCTACAATAACTTTTCTGGTACATTTCCTGTAAGCTTAAACAGCTTGACTGAGCTAAGCAAGTTCAATATTTCATACAATCCACTCATCTCTGGTGTTATTCCTTCAAGTGGCCAATTGGCAACGTTTGAAAAGGAGTCCTACCTTGGTGACCCCTTCCTTACTCTTCCGAGTTTTATCACCAACTCCCCGAATAATTCAACAAACAAGACAACTGGAAACCAAAAAAAGCCTGCAAAGCTTGGTGCTAATTTGGTGTTTCTAGTGCTAGTACTGACTTTCTTGATATGTGGGGTCTTTTCACTTACGATGTGCTTATTTATGAAGAGCCCAGCAGATGAGCCACAAGGGTATCTGCTGCCACACAGCAAATACCGGCATGACCTTGCATCAAGTTCAAACTCTTCATCACCTTGGCTATCGGATACTGTCAAGGTCATTCGACTGGACAAAACAGCTTTCACACATGCTGACATTTTGAAGGCCACAGGCAATTTTTCAGAAGGGAGGATCATAGGGAGGGGAGGGTTTGGAACAGTGTACCAAGGAGTGTTGCCTGATGGGAGAGAAGTAGCAGTGAAGAAGCTTCAAAGAGAAGGACTTGAAGGTGAAAGGGAATTCAGAGCTGAAATGGAAGTTCTTACTGGAAATGGCTTTGGTTGGCCTCACCCAAATCTTGTGCAACTTCATGGTTGGTGTCATGATGGTTCAGAGAAAATATTAGTCTATGAGTACATGGAAGGTGGGAGCTTGGAGGATATTATATGTGACAAAGTAAGACTAAGATGGCGCAGACGGATTGATGTAGCAGTTGATGTGGCTCGAGCCTTGGTGTTTCTACACCATGAGTGCTTCCCTGCTATCGTGCACCGTGATGTGAAGGCTAGCAATGTGCTGCTAGATAAGGATGGGAAGGCAAGAGTGACAGATTTTGGGCTGGCTAGAATTGTTGATGCAGGGGATAGTCATGTGAGCACAATGGTGGCTGGAACTATTGGTTATGTTGCACCAGAATATGGGCAGACATGGCAAGCCACAACGAAAGGCGATGTGTACAGTTATGGAGTCCTAGCAATGGAACTGGCAACTGGGAGGAGAGCAGTGGATGGAGGAGAGGAGTGTCTAGTGGAATGGGCCAGAAGGGTGATGGGAAATGGGCGGCTAGGATTCAATCGTTCTGTGATGCCGGTTGTGCTTATGGGGTCTGGACTAGTTGATGGGGCAGAAGAGATGTGTGAGTTACTAAGGGTTGGCATAAAGTGCACAGCTGAGGCACCACAGTCTAGACCAAACATGAAGGAAGTTCTAGCTATGCTGATAAAGATAGCCAATATCCAAGGGGATTTTAGTTGTGGTTCTCCTCCTTCATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

1099

Amino Acids

120.75

Weight (kDa)

6.18

Isoelectric Point (pI)

37.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 100 - 139 2.5e-06 Leucine rich repeat
LRR_8 PF13855 343 - 403 1.2e-07 Leucine rich repeat
LRR_14 PF23598 386 - 474 1.9e-09 Leucine-rich repeat region
LRR_8 PF13855 416 - 475 2.8e-07 Leucine rich repeat
Pkinase PF00069 809 - 1078 8.9e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 811 - 1080 1e-40 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1018
Acc16I TGCGCA 1 cut(s) 1763
Acc36I ACCTGC 2 cut(s) 1018, 1460
AccB1I GGYRCC 1 cut(s) 3199
AccB7I CCANNNNNTGG 4 cut(s) 1232, 1258, 1751, 2942
AciI CCGC 1 cut(s) 3085
AclI AACGTT 1 cut(s) 2051
AclWI GGATC 4 cut(s) 1515, 1528, 1632, 2444
AcoI YGGCCR 2 cut(s) 1296, 2038
AcuI CTGAAG 1 cut(s) 102
AcyI GRCGYC 1 cut(s) 683
AfiI CCNNNNNNNGG 9 cut(s) 213, 703, 814, 1232, 1258, 1751, 2036, 2942, 3001
AgeI ACCGGT 1 cut(s) 1150
AhlI ACTAGT 1 cut(s) 3136
AjnI CCWGG 2 cut(s) 327, 514
AjuI GAANNNNNNNTTGG 2 cut(s) 781, 813
AleI CACNNNNGTG 2 cut(s) 699, 2906
AloI GAACNNNNNNTCC 2 cut(s) 881, 913
Alw21I GWGCWC 6 cut(s) 47, 599, 626, 2796, 2903, 3191
Alw26I GTCTC 1 cut(s) 1323
Alw44I GTGCAC 2 cut(s) 2792, 3187
AlwI GGATC 4 cut(s) 1515, 1528, 1632, 2444
Ama87I CYCGRG 2 cut(s) 1870, 2748
AoxI GGCC 9 cut(s) 172, 215, 851, 1296, 1645, 2038, 2409, 2588, 3060
ApaLI GTGCAC 2 cut(s) 2792, 3187
ApeKI GCWGC 4 cut(s) 818, 1045, 2284, 2821
ArsI GACNNNNNNTTYG 4 cut(s) 304, 336, 569, 601
AsiGI ACCGGT 1 cut(s) 1150
Asp700I GAANNNNTTC 3 cut(s) 770, 877, 1865
AspLEI GCGC 3 cut(s) 33, 1764, 2718
AspS9I GGNCC 8 cut(s) 325, 754, 851, 1142, 1261, 1472, 1738, 3060
AsuC2I CCSGG 4 cut(s) 365, 932, 1295, 1525
AsuNHI GCTAGC 1 cut(s) 2810
AvaI CYCGRG 2 cut(s) 1870, 2748
AvaII GGWCC 6 cut(s) 325, 754, 1142, 1261, 1472, 1738
BaeGI GKGCMC 2 cut(s) 2796, 3191
BaeI ACNNNNGTAYC 2 cut(s) 2261, 2294
BalI TGGCCA 1 cut(s) 2040
BamHI GGATCC 1 cut(s) 1520
BanI GGYRCC 1 cut(s) 3199
BanII GRGCYC 1 cut(s) 2258
BarI GAAGNNNNNNTAC 2 cut(s) 1172, 1204
Bbv12I GWGCWC 6 cut(s) 47, 599, 626, 2796, 2903, 3191
BbvCI CCTCAGC 1 cut(s) 3195
BbvI GCAGC 4 cut(s) 805, 1032, 2271, 2808
BciT130I CCWGG 2 cut(s) 329, 516
BciVI GTATCC 1 cut(s) 2344
BcnI CCSGG 4 cut(s) 365, 932, 1295, 1525
BcoDI GTCTC 1 cut(s) 1323
BcuI ACTAGT 1 cut(s) 3136
BfoI RGCGCY 1 cut(s) 34
BfuAI ACCTGC 2 cut(s) 1018, 1460
BfuI GTATCC 1 cut(s) 2344
BglII AGATCT 1 cut(s) 1117
BisI GCNGC 5 cut(s) 819, 1046, 2285, 2822, 3086
BlpI GCTNAGC 3 cut(s) 128, 1534, 1982
BlsI GCNGC 5 cut(s) 820, 1047, 2286, 2823, 3087
BmcAI AGTACT 4 cut(s) 584, 611, 1184, 2199
Bme1390I CCNGG 6 cut(s) 329, 365, 516, 932, 1295, 1525
Bme18I GGWCC 6 cut(s) 325, 754, 1142, 1261, 1472, 1738
BmeT110I CYCGRG 2 cut(s) 1870, 2748
BmgT120I GGNCC 8 cut(s) 325, 754, 851, 1142, 1261, 1472, 1738, 3060
BmiI GGNNCC 5 cut(s) 191, 1522, 1721, 1740, 3201
BmrFI CCNGG 6 cut(s) 329, 365, 516, 932, 1295, 1525
BmrI ACTGGG 1 cut(s) 3024
BmsI GCATC 6 cut(s) 1323, 1495, 1749, 2324, 2869, 3099
BmtI GCTAGC 1 cut(s) 2814
BmuI ACTGGG 1 cut(s) 3024
BoxI GACNNNNGTC 1 cut(s) 375
BpmI CTGGAG 3 cut(s) 114, 908, 1728
Bpu10I CCTNAGC 2 cut(s) 668, 3195
Bpu1102I GCTNAGC 3 cut(s) 128, 1534, 1982
BpuEI CTTGAG 3 cut(s) 377, 394, 1076
BpuMI CCSGG 4 cut(s) 365, 932, 1295, 1525
Bsa29I ATCGAT 1 cut(s) 258
BsaHI GRCGYC 1 cut(s) 683
BsaI GGTCTC 1 cut(s) 1323
BsaJI CCNNGG 8 cut(s) 169, 514, 1524, 2071, 2339, 2470, 2754, 3262
BsaWI WCCGGW 1 cut(s) 1150
BsaXI ACNNNNNCTCC 2 cut(s) 881, 911
Bsc4I CCNNNNNNNGG 9 cut(s) 213, 703, 814, 1232, 1258, 1751, 2036, 2942, 3001
Bse118I RCCGGY 4 cut(s) 1150, 1817, 2301, 3112
Bse1I ACTGG 9 cut(s) 256, 571, 580, 1263, 2146, 2376, 2575, 3012, 3019
Bse3DI GCAATG 4 cut(s) 238, 1685, 2821, 3006
BseBI CCWGG 2 cut(s) 329, 516
BseCI ATCGAT 1 cut(s) 258
BseDI CCNNGG 8 cut(s) 169, 514, 1524, 2071, 2339, 2470, 2754, 3262
BseGI GGATG 4 cut(s) 927, 1510, 2839, 3037
BseLI CCNNNNNNNGG 9 cut(s) 213, 703, 814, 1232, 1258, 1751, 2036, 2942, 3001
BseMI GCAATG 4 cut(s) 238, 1685, 2821, 3006
BseNI ACTGG 9 cut(s) 256, 571, 580, 1263, 2146, 2376, 2575, 3012, 3019
BseRI GAGGAG 4 cut(s) 3034, 3051, 3056, 3276
BseSI GKGCMC 2 cut(s) 2796, 3191
BseXI GCAGC 4 cut(s) 805, 1032, 2271, 2808
BseYI CCCAGC 1 cut(s) 2257
BsgI GTGCAG 1 cut(s) 1031
BshFI GGCC 9 cut(s) 174, 217, 853, 1298, 1647, 2040, 2411, 2590, 3062
BshNI GGYRCC 1 cut(s) 3199
BshTI ACCGGT 1 cut(s) 1150
BshVI ATCGAT 1 cut(s) 258
BsiHKAI GWGCWC 6 cut(s) 47, 599, 626, 2796, 2903, 3191
BsiHKCI CYCGRG 2 cut(s) 1870, 2748
BsiSI CCGG 9 cut(s) 365, 808, 932, 1151, 1295, 1524, 1818, 2302, 3113
BslFI GGGAC 2 cut(s) 1474, 1724
BslI CCNNNNNNNGG 9 cut(s) 213, 703, 814, 1232, 1258, 1751, 2036, 2942, 3001
BsmAI GTCTC 1 cut(s) 1323
BsmFI GGGAC 2 cut(s) 1474, 1724
BsnI GGCC 9 cut(s) 174, 217, 853, 1298, 1647, 2040, 2411, 2590, 3062
Bso31I GGTCTC 1 cut(s) 1323
BsoBI CYCGRG 2 cut(s) 1870, 2748
Bsp1286I GDGCHC 7 cut(s) 47, 599, 626, 2258, 2796, 2903, 3191
Bsp1407I TGTACA 3 cut(s) 1584, 1705, 2979
Bsp143I GATC 6 cut(s) 259, 1117, 1509, 1520, 1624, 2436
Bsp1720I GCTNAGC 3 cut(s) 128, 1534, 1982
BspACI CCGC 1 cut(s) 3085
BspANI GGCC 9 cut(s) 174, 217, 853, 1298, 1647, 2040, 2411, 2590, 3062
BspDI ATCGAT 1 cut(s) 258
BspHI TCATGA 2 cut(s) 69, 2626
BspLI GGNNCC 5 cut(s) 191, 1522, 1721, 1740, 3201
BspMI ACCTGC 2 cut(s) 1018, 1460
BspOI GCTAGC 1 cut(s) 2814
BspPI GGATC 4 cut(s) 1515, 1528, 1632, 2444
BspQI GCTCTTC 2 cut(s) 52, 2246
BspT107I GGYRCC 1 cut(s) 3199
BspTNI GGTCTC 1 cut(s) 1323
BsrDI GCAATG 4 cut(s) 238, 1685, 2821, 3006
BsrFI RCCGGY 4 cut(s) 1150, 1817, 2301, 3112
BsrGI TGTACA 3 cut(s) 1584, 1705, 2979
BsrI ACTGG 9 cut(s) 256, 571, 580, 1263, 2146, 2376, 2575, 3012, 3019
BssAI RCCGGY 4 cut(s) 1150, 1817, 2301, 3112
BssECI CCNNGG 8 cut(s) 169, 514, 1524, 2071, 2339, 2470, 2754, 3262
BssMI GATC 6 cut(s) 259, 1117, 1509, 1520, 1624, 2436
BssNI GRCGYC 1 cut(s) 683
BssT1I CCWWGG 6 cut(s) 169, 2071, 2339, 2470, 2754, 3262
Bst2UI CCWGG 2 cut(s) 329, 516
Bst4CI ACNGT 9 cut(s) 242, 914, 1865, 1901, 2356, 2464, 2798, 2984, 3207
Bst6I CTCTTC 6 cut(s) 52, 1598, 2097, 2246, 2335, 3147
BstACI GRCGYC 1 cut(s) 683
BstAPI GCANNNNNTGC 1 cut(s) 3186
BstAUI TGTACA 3 cut(s) 1584, 1705, 2979
BstC8I GCNNGC 5 cut(s) 1338, 2160, 2812, 2865, 2958
BstEII GGTNACC 1 cut(s) 2075
BstF5I GGATG 4 cut(s) 927, 1510, 2839, 3037
BstH2I RGCGCY 1 cut(s) 34
BstHHI GCGC 3 cut(s) 33, 1764, 2718
BstKTI GATC 6 cut(s) 262, 1120, 1512, 1523, 1627, 2439
BstMAI GTCTC 1 cut(s) 1323
BstMBI GATC 6 cut(s) 259, 1117, 1509, 1520, 1624, 2436
BstMWI GCNNNNNNNGC 9 cut(s) 439, 1024, 1673, 1761, 1968, 2164, 2953, 3118, 3186
BstNI CCWGG 2 cut(s) 329, 516
BstNSI RCATGY 2 cut(s) 1621, 2396
BstPAI GACNNNNGTC 1 cut(s) 375
BstPI GGTNACC 1 cut(s) 2075
BstSCI CCNGG 6 cut(s) 327, 363, 514, 930, 1293, 1523
BstSLI GKGCMC 2 cut(s) 2796, 3191
BstV1I GCAGC 4 cut(s) 805, 1032, 2271, 2808
BstX2I RGATCY 2 cut(s) 1117, 1520
BstXI CCANNNNNNTGG 1 cut(s) 115
BstYI RGATCY 2 cut(s) 1117, 1520
Bsu15I ATCGAT 1 cut(s) 258
BsuI GTATCC 1 cut(s) 2344
BsuRI GGCC 9 cut(s) 174, 217, 853, 1298, 1647, 2040, 2411, 2590, 3062
BsuTUI ATCGAT 1 cut(s) 258
BtgZI GCGATG 1 cut(s) 2988
BtsCI GGATG 4 cut(s) 927, 1510, 2839, 3037
BtsI GCAGTG 2 cut(s) 2508, 3033
BtsIMutI CAGTG 6 cut(s) 706, 1216, 1256, 2469, 2508, 3033
BveI ACCTGC 2 cut(s) 1018, 1460
Cac8I GCNNGC 5 cut(s) 1338, 2160, 2812, 2865, 2958
CciI TCATGA 2 cut(s) 69, 2626
CfoI GCGC 3 cut(s) 33, 1764, 2718
Cfr10I RCCGGY 4 cut(s) 1150, 1817, 2301, 3112
Cfr13I GGNCC 8 cut(s) 325, 754, 851, 1142, 1261, 1472, 1738, 3060
ClaI ATCGAT 1 cut(s) 258
CseI GACGC 1 cut(s) 672
CspAI ACCGGT 1 cut(s) 1150
CspCI CAANNNNNGTGG 2 cut(s) 619, 654
DpnI GATC 6 cut(s) 261, 1119, 1511, 1522, 1626, 2438
DpnII GATC 6 cut(s) 259, 1117, 1509, 1520, 1624, 2436
EaeI YGGCCR 2 cut(s) 1296, 2038
Eam1104I CTCTTC 6 cut(s) 52, 1598, 2097, 2246, 2335, 3147
EarI CTCTTC 6 cut(s) 52, 1598, 2097, 2246, 2335, 3147
Eco130I CCWWGG 6 cut(s) 169, 2071, 2339, 2470, 2754, 3262
Eco24I GRGCYC 1 cut(s) 2258
Eco31I GGTCTC 1 cut(s) 1323
Eco32I GATATC 1 cut(s) 1498
Eco47I GGWCC 6 cut(s) 325, 754, 1142, 1261, 1472, 1738
Eco57I CTGAAG 1 cut(s) 102
Eco88I CYCGRG 2 cut(s) 1870, 2748
Eco91I GGTNACC 1 cut(s) 2075
EcoO109I RGGNCCY 2 cut(s) 1142, 1738
EcoO65I GGTNACC 1 cut(s) 2075
EcoRI GAATTC 2 cut(s) 1086, 2543
EcoRII CCWGG 2 cut(s) 327, 514
EcoRV GATATC 1 cut(s) 1498
EcoT14I CCWWGG 6 cut(s) 169, 2071, 2339, 2470, 2754, 3262
EcoT38I GRGCYC 1 cut(s) 2258
ErhI CCWWGG 6 cut(s) 169, 2071, 2339, 2470, 2754, 3262
FalI AAGNNNNNCTT 4 cut(s) 129, 161, 494, 526
FaqI GGGAC 2 cut(s) 1474, 1724
FauNDI CATATG 1 cut(s) 1658
Fnu4HI GCNGC 5 cut(s) 819, 1046, 2285, 2822, 3086
FokI GGATG 4 cut(s) 914, 1517, 2846, 3044
FriOI GRGCYC 1 cut(s) 2258
Fsp4HI GCNGC 5 cut(s) 819, 1046, 2285, 2822, 3086
FspI TGCGCA 1 cut(s) 1763
GlaI GCGC 3 cut(s) 32, 1763, 2717
GluI GCNGC 5 cut(s) 819, 1046, 2285, 2822, 3086
GsaI CCCAGC 1 cut(s) 2261
GsuI CTGGAG 3 cut(s) 114, 908, 1728
HaeII RGCGCY 1 cut(s) 34
HaeIII GGCC 9 cut(s) 174, 217, 853, 1298, 1647, 2040, 2411, 2590, 3062
HapII CCGG 9 cut(s) 365, 808, 932, 1151, 1295, 1524, 1818, 2302, 3113
HgaI GACGC 1 cut(s) 672
HhaI GCGC 3 cut(s) 33, 1764, 2718
Hin1I GRCGYC 1 cut(s) 683
Hin6I GCGC 3 cut(s) 31, 1762, 2716
HinP1I GCGC 3 cut(s) 31, 1762, 2716
HindIII AAGCTT 5 cut(s) 138, 1174, 1960, 2165, 2510
HpaII CCGG 9 cut(s) 365, 808, 932, 1151, 1295, 1524, 1818, 2302, 3113
Hpy166II GTNNAC 7 cut(s) 166, 1585, 1587, 2467, 2794, 2980, 3189
Hpy8I GTNNAC 7 cut(s) 166, 1585, 1587, 2467, 2794, 2980, 3189
HpyCH4III ACNGT 9 cut(s) 242, 914, 1865, 1901, 2356, 2464, 2798, 2984, 3207
HpyCH4IV ACGT 1 cut(s) 2051
HpyF10VI GCNNNNNNNGC 9 cut(s) 439, 1024, 1673, 1761, 1968, 2164, 2953, 3118, 3186
HpySE526I ACGT 1 cut(s) 2051
Hsp92I GRCGYC 1 cut(s) 683
HspAI GCGC 3 cut(s) 31, 1762, 2716
Kzo9I GATC 6 cut(s) 259, 1117, 1509, 1520, 1624, 2436
LguI GCTCTTC 2 cut(s) 52, 2246
LmnI GCTCC 4 cut(s) 356, 1254, 1372, 2673
Lsp1109I GCAGC 4 cut(s) 805, 1032, 2271, 2808
LweI GCATC 6 cut(s) 1323, 1495, 1749, 2324, 2869, 3099
MaeII ACGT 1 cut(s) 2051
MalI GATC 6 cut(s) 261, 1119, 1511, 1522, 1626, 2438
MboI GATC 6 cut(s) 259, 1117, 1509, 1520, 1624, 2436
MfeI CAATTG 3 cut(s) 455, 1829, 2042
MflI RGATCY 2 cut(s) 1117, 1520
MhlI GDGCHC 7 cut(s) 47, 599, 626, 2258, 2796, 2903, 3191
MlsI TGGCCA 1 cut(s) 2040
MluNI TGGCCA 1 cut(s) 2040
MlyI GAGTC 6 cut(s) 385, 460, 892, 933, 2072, 3000
MmeI TCCRAC 3 cut(s) 634, 1437, 1499
Mox20I TGGCCA 1 cut(s) 2040
MroXI GAANNNNTTC 3 cut(s) 770, 877, 1865
MscI TGGCCA 1 cut(s) 2040
MseI TTAA 5 cut(s) 135, 1635, 1799, 1848, 1964
MslI CAYNNNNRTG 5 cut(s) 68, 113, 699, 2799, 2906
Msp20I TGGCCA 1 cut(s) 2040
MspA1I CMGCKG 2 cut(s) 80, 3194
MspI CCGG 9 cut(s) 365, 808, 932, 1151, 1295, 1524, 1818, 2302, 3113
MspR9I CCNGG 6 cut(s) 329, 365, 516, 932, 1295, 1525
MunI CAATTG 3 cut(s) 455, 1829, 2042
MvaI CCWGG 2 cut(s) 329, 516
MwoI GCNNNNNNNGC 9 cut(s) 439, 1024, 1673, 1761, 1968, 2164, 2953, 3118, 3186
NciI CCSGG 4 cut(s) 365, 932, 1295, 1525
NdeI CATATG 1 cut(s) 1658
NdeII GATC 6 cut(s) 259, 1117, 1509, 1520, 1624, 2436
NheI GCTAGC 1 cut(s) 2810
NlaIV GGNNCC 5 cut(s) 191, 1522, 1721, 1740, 3201
NmuCI GTSAC 6 cut(s) 236, 408, 759, 2075, 2693, 2848
NsbI TGCGCA 1 cut(s) 1763
NspI RCATGY 2 cut(s) 1621, 2396
OliI CACNNNNGTG 2 cut(s) 699, 2906
PaeR7I CTCGAG 1 cut(s) 2748
PagI TCATGA 2 cut(s) 69, 2626
PaqCI CACCTGC 1 cut(s) 1018
PciSI GCTCTTC 2 cut(s) 52, 2246
PcsI WCGNNNNNNNCGW 1 cut(s) 264
PdmI GAANNNNTTC 3 cut(s) 770, 877, 1865
PflMI CCANNNNNTGG 4 cut(s) 1232, 1258, 1751, 2942
PfoI TCCNGGA 2 cut(s) 363, 930
PinAI ACCGGT 1 cut(s) 1150
PkrI GCNGC 5 cut(s) 820, 1047, 2286, 2823, 3087
PleI GAGTC 6 cut(s) 384, 459, 891, 932, 2071, 2999
PpsI GAGTC 6 cut(s) 384, 459, 891, 932, 2071, 2999
PpuMI RGGWCCY 2 cut(s) 1142, 1738
PshAI GACNNNNGTC 1 cut(s) 375
Psp1406I AACGTT 1 cut(s) 2051
Psp5II RGGWCCY 2 cut(s) 1142, 1738
Psp6I CCWGG 2 cut(s) 327, 514
PspEI GGTNACC 1 cut(s) 2075
PspFI CCCAGC 1 cut(s) 2257
PspGI CCWGG 2 cut(s) 327, 514
PspN4I GGNNCC 5 cut(s) 191, 1522, 1721, 1740, 3201
PspPI GGNCC 8 cut(s) 325, 754, 851, 1142, 1261, 1472, 1738, 3060
PspPPI RGGWCCY 2 cut(s) 1142, 1738
PspXI VCTCGAGB 1 cut(s) 2748
PsuI RGATCY 2 cut(s) 1117, 1520
PvuII CAGCTG 2 cut(s) 80, 3194
RseI CAYNNNNRTG 5 cut(s) 68, 113, 699, 2799, 2906
SapI GCTCTTC 2 cut(s) 52, 2246
SaqAI TTAA 5 cut(s) 135, 1635, 1799, 1848, 1964
SatI GCNGC 5 cut(s) 819, 1046, 2285, 2822, 3086
Sau3AI GATC 6 cut(s) 259, 1117, 1509, 1520, 1624, 2436
Sau96I GGNCC 8 cut(s) 325, 754, 851, 1142, 1261, 1472, 1738, 3060
ScaI AGTACT 4 cut(s) 584, 611, 1184, 2199
SchI GAGTC 6 cut(s) 385, 460, 892, 933, 2072, 3000
ScrFI CCNGG 6 cut(s) 329, 365, 516, 932, 1295, 1525
SduI GDGCHC 7 cut(s) 47, 599, 626, 2258, 2796, 2903, 3191
SfaNI GCATC 6 cut(s) 1323, 1495, 1749, 2324, 2869, 3099
Sfr274I CTCGAG 1 cut(s) 2748
SinI GGWCC 6 cut(s) 325, 754, 1142, 1261, 1472, 1738
SlaI CTCGAG 1 cut(s) 2748
SmiMI CAYNNNNRTG 5 cut(s) 68, 113, 699, 2799, 2906
SmlI CTYRAG 4 cut(s) 373, 392, 1091, 2748
SmoI CTYRAG 4 cut(s) 373, 392, 1091, 2748
SpeI ACTAGT 1 cut(s) 3136
SsiI CCGC 1 cut(s) 3085
SspI AATATT 3 cut(s) 1456, 1996, 2646
StyD4I CCNGG 6 cut(s) 327, 363, 514, 930, 1293, 1523
StyI CCWWGG 6 cut(s) 169, 2071, 2339, 2470, 2754, 3262
TaaI ACNGT 9 cut(s) 242, 914, 1865, 1901, 2356, 2464, 2798, 2984, 3207
TaiI ACGT 1 cut(s) 2054
TaqI TCGA 9 cut(s) 8, 14, 258, 599, 781, 881, 1612, 2368, 2749
TauI GCSGC 1 cut(s) 3088
Tru1I TTAA 5 cut(s) 135, 1635, 1799, 1848, 1964
Tru9I TTAA 5 cut(s) 135, 1635, 1799, 1848, 1964
TscAI CASTG 6 cut(s) 706, 1216, 1263, 2469, 2508, 3033
TseFI GTSAC 6 cut(s) 236, 408, 759, 2075, 2693, 2848
TseI GCWGC 4 cut(s) 818, 1045, 2284, 2821
Tsp45I GTSAC 6 cut(s) 236, 408, 759, 2075, 2693, 2848
TspGWI ACGGA 1 cut(s) 2737
TspRI CASTG 6 cut(s) 706, 1216, 1263, 2469, 2508, 3033
Van91I CCANNNNNTGG 4 cut(s) 1232, 1258, 1751, 2942
VneI GTGCAC 2 cut(s) 2792, 3187
VpaK11BI GGWCC 6 cut(s) 325, 754, 1142, 1261, 1472, 1738
XbaI TCTAGA 3 cut(s) 421, 920, 3209
XceI RCATGY 2 cut(s) 1621, 2396
XcmI CCANNNNNNNNNTGG 1 cut(s) 3070
XhoI CTCGAG 1 cut(s) 2748
XmnI GAANNNNTTC 3 cut(s) 770, 877, 1865
ZrmI AGTACT 4 cut(s) 584, 611, 1184, 2199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.