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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 24 |
| AciI | CCGC | 1 cut(s) | 24 |
| AcsI | RAATTY | 1 cut(s) | 230 |
| AgsI | TTSAA | 2 cut(s) | 14, 148 |
| Alw26I | GTCTC | 1 cut(s) | 56 |
| ApoI | RAATTY | 1 cut(s) | 230 |
| AspLEI | GCGC | 1 cut(s) | 24 |
| BcoDI | GTCTC | 1 cut(s) | 56 |
| BfaI | CTAG | 2 cut(s) | 45, 219 |
| BfmI | CTRYAG | 1 cut(s) | 130 |
| BmsI | GCATC | 1 cut(s) | 50 |
| BpuEI | CTTGAG | 1 cut(s) | 38 |
| BseGI | GGATG | 1 cut(s) | 219 |
| Bsh1236I | CGCG | 1 cut(s) | 24 |
| BsmAI | GTCTC | 1 cut(s) | 56 |
| Bsp143I | GATC | 4 cut(s) | 60, 134, 173, 187 |
| BspACI | CCGC | 1 cut(s) | 24 |
| BspFNI | CGCG | 1 cut(s) | 24 |
| BspMAI | CTGCAG | 1 cut(s) | 134 |
| BssMI | GATC | 4 cut(s) | 60, 134, 173, 187 |
| BstF5I | GGATG | 1 cut(s) | 219 |
| BstFNI | CGCG | 1 cut(s) | 24 |
| BstHHI | GCGC | 1 cut(s) | 24 |
| BstKTI | GATC | 4 cut(s) | 63, 137, 176, 190 |
| BstMAI | GTCTC | 1 cut(s) | 56 |
| BstMBI | GATC | 4 cut(s) | 60, 134, 173, 187 |
| BstSFI | CTRYAG | 1 cut(s) | 130 |
| BstUI | CGCG | 1 cut(s) | 24 |
| BtsCI | GGATG | 1 cut(s) | 219 |
| BtsI | GCAGTG | 1 cut(s) | 127 |
| BtsIMutI | CAGTG | 1 cut(s) | 127 |
| CfoI | GCGC | 1 cut(s) | 24 |
| DpnI | GATC | 4 cut(s) | 62, 136, 175, 189 |
| DpnII | GATC | 4 cut(s) | 60, 134, 173, 187 |
| DraI | TTTAAA | 1 cut(s) | 93 |
| FaiI | YATR | 5 cut(s) | 33, 186, 195, 197, 238 |
| FokI | GGATG | 1 cut(s) | 226 |
| FspBI | CTAG | 2 cut(s) | 45, 219 |
| GlaI | GCGC | 1 cut(s) | 23 |
| HhaI | GCGC | 1 cut(s) | 24 |
| Hin6I | GCGC | 1 cut(s) | 22 |
| HinP1I | GCGC | 1 cut(s) | 22 |
| HincII | GTYRAC | 1 cut(s) | 142 |
| HindII | GTYRAC | 1 cut(s) | 142 |
| HinfI | GANTC | 1 cut(s) | 113 |
| Hpy166II | GTNNAC | 1 cut(s) | 142 |
| Hpy188I | TCNGA | 3 cut(s) | 118, 171, 178 |
| Hpy188III | TCNNGA | 3 cut(s) | 45, 55, 226 |
| Hpy8I | GTNNAC | 1 cut(s) | 142 |
| HpyCH4V | TGCA | 1 cut(s) | 132 |
| HspAI | GCGC | 1 cut(s) | 22 |
| Kzo9I | GATC | 4 cut(s) | 60, 134, 173, 187 |
| LweI | GCATC | 1 cut(s) | 50 |
| MaeI | CTAG | 2 cut(s) | 45, 219 |
| MalI | GATC | 4 cut(s) | 62, 136, 175, 189 |
| MboI | GATC | 4 cut(s) | 60, 134, 173, 187 |
| MboII | GAAGA | 2 cut(s) | 39, 70 |
| MluCI | AATT | 4 cut(s) | 71, 82, 181, 230 |
| MmeI | TCCRAC | 1 cut(s) | 181 |
| MseI | TTAA | 1 cut(s) | 92 |
| MvnI | CGCG | 1 cut(s) | 24 |
| NdeII | GATC | 4 cut(s) | 60, 134, 173, 187 |
| PfeI | GAWTC | 1 cut(s) | 113 |
| PstI | CTGCAG | 1 cut(s) | 134 |
| SaqAI | TTAA | 1 cut(s) | 92 |
| Sau3AI | GATC | 4 cut(s) | 60, 134, 173, 187 |
| SfaNI | GCATC | 1 cut(s) | 50 |
| SfcI | CTRYAG | 1 cut(s) | 130 |
| SgeI | CNNG | 5 cut(s) | 30, 35, 57, 67, 231 |
| SmlI | CTYRAG | 1 cut(s) | 53 |
| SmoI | CTYRAG | 1 cut(s) | 53 |
| Sse9I | AATT | 4 cut(s) | 71, 82, 181, 230 |
| SsiI | CCGC | 1 cut(s) | 24 |
| SspMI | CTAG | 2 cut(s) | 45, 219 |
| TaqII | GACCGA | 1 cut(s) | 155 |
| TasI | AATT | 4 cut(s) | 71, 82, 181, 230 |
| TfiI | GAWTC | 1 cut(s) | 113 |
| Tru1I | TTAA | 1 cut(s) | 92 |
| Tru9I | TTAA | 1 cut(s) | 92 |
| TscAI | CASTG | 1 cut(s) | 134 |
| TspGWI | ACGGA | 2 cut(s) | 165, 226 |
| TspRI | CASTG | 1 cut(s) | 134 |
| XapI | RAATTY | 1 cut(s) | 230 |
| XbaI | TCTAGA | 1 cut(s) | 44 |
| XspI | CTAG | 2 cut(s) | 45, 219 |