Rorug06G0002200

Phospholipase A-2-activating

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0002857
Physical Location & Seq
Reverse (-)
417374 .. 418858
1485 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0002200.1

Sequence Viewer

Length: 1044 bp
ATGGGATTTGGGGAGTCAGTGGCGGCGGCGGCGCAGGTTCCGGCAATGTTTGCGTTTGGGGACTCACTCATTGATGCTGGCAACAACAACTTCATAAGCTCCATCGCAAAATCCAATTATTATCCTTACGGCATCGATTTCAGGGGAGGCCCCTCCGGCAGATTCACCAATGGAAGAACTTTCGTTGATATGCTTGGAAATTTGCTGGGTCTTCCTTATGCTCCAGCCTTTGCAGATCCTAATACAAAAGGAAACACAATGCTCAGGGGAGTCAATTATGCTTCAGCAGCTGCTGGCATACTCGATGAGACAGGCCGGCACTATGGAGATCGGTATACCCTAAGCCGGCAAGTGTTGAATTTCGAGACCACCTTGAATCAGTTAAGAACGATGATGGGGGGAACTAACCTGACCCAGTACCTGGCTAAGTCGATTGCCGTTTTGGTATTTGGGAGCAATGACTACATCAACAACTACCTCATGCCTTCCCTCTATTCTTCCAGCTACACTTACACTCCTCCTGCCTTTGCCAACCTTCTCCTTAATCGCTATGCCACACAGATTCTGGCTTTGCACGGTCTTGGATTGAGGAAGTTTGTGCTAGTAGGAATTGGGCCTTTGGGTTGCATTCCTAACCAAAGAGCATCAGGTAAGGCCCAGCCTGGAAGGTGTGTTGACAATGTCAATCAGATCCTTGGCAGCTTCAATCAAGGGCTCAGATCGCTCGTCACCCAACTTAACAACAACCATCCTGGTGCCATCTTTGTGTACGGCAATGCTTATGGCGCTTTTGGTGACATTCTGAATAACCCTGCACCCTATGGTTTCAGTGTGATTGATAGGGCTTGCTGTGGAATTGGGAGGAACCAAGGGCAGATAACTTGTCTTCCATTTGCAGTTCCATGCTCAAATAGAAATCAATATATGTTTTGGGATGCTTTCCACCCAACTGAAGCTGCAAACGCAGTCCTTGCTTGGCGAGCTTTTAATGGCCCGCCCTCTGATAGTTACCCCATCAATGTCCAACAAATGGCTCTCATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001558 GO:0002237 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006082 GO:0006508 GO:0006511 GO:0006629 GO:0006631 GO:0006690 GO:0006692 GO:0006693 GO:0006807 GO:0006914 GO:0007275 GO:0007399 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009605 GO:0009607 GO:0009617 GO:0009892 GO:0009987 GO:0010033 GO:0010517 GO:0010518 GO:0010605 GO:0010720 GO:0010975 GO:0010976 GO:0016236 GO:0019222 GO:0019538 GO:0019752 GO:0019941 GO:0022008 GO:0022603 GO:0022604 GO:0030154 GO:0030163 GO:0030307 GO:0030334 GO:0030335 GO:0031323 GO:0031324 GO:0031344 GO:0031346 GO:0031396 GO:0031397 GO:0031399 GO:0031400 GO:0032268 GO:0032269 GO:0032429 GO:0032430 GO:0032496 GO:0032501 GO:0032502 GO:0032787 GO:0032879 GO:0033559 GO:0033993 GO:0040008 GO:0040012 GO:0040017 GO:0042221 GO:0043085 GO:0043162 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043632 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044255 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044281 GO:0044424 GO:0044464 GO:0045202 GO:0045595 GO:0045597 GO:0045664 GO:0045666 GO:0045927 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048638 GO:0048639 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050767 GO:0050769 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050896 GO:0051049 GO:0051050 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051239 GO:0051240 GO:0051246 GO:0051248 GO:0051270 GO:0051272 GO:0051336 GO:0051345 GO:0051603 GO:0051704 GO:0051707 GO:0051716 GO:0051960 GO:0051962 GO:0060191 GO:0060193 GO:0060255 GO:0060284 GO:0060341 GO:0061919 GO:0065007 GO:0065009 GO:0070887 GO:0071216 GO:0071219 GO:0071222 GO:0071310 GO:0071396 GO:0071704 GO:0080090 GO:0098693 GO:0120035 GO:1900044 GO:1900045 GO:1901564 GO:1901565 GO:1901568 GO:1901575 GO:1901700 GO:1901701 GO:1902914 GO:1902915 GO:1903320 GO:1903321 GO:1903421 GO:1903423 GO:1903859 GO:1903861 GO:2000026 GO:2000145 GO:2000147 GO:2001222 GO:2001224
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
PFU PUL WD40

Protein Analysis

347

Amino Acids

37.49

Weight (kDa)

8.84

Isoelectric Point (pI)

26.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 17 - 325 7.4e-33 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 25
AccB1I GGYRCC 1 cut(s) 755
AccB7I CCANNNNNTGG 2 cut(s) 421, 1030
AccI GTMKAC 1 cut(s) 335
AciI CCGC 4 cut(s) 23, 26, 29, 995
AclWI GGATC 2 cut(s) 230, 685
AcsI RAATTY 2 cut(s) 199, 358
AcuI CTGAAG 2 cut(s) 267, 972
AfaI GTAC 2 cut(s) 419, 770
AfiI CCNNNNNNNGG 3 cut(s) 345, 421, 1030
AgsI TTSAA 3 cut(s) 358, 376, 706
AjnI CCWGG 3 cut(s) 420, 661, 751
AluBI AGCT 6 cut(s) 99, 290, 504, 702, 956, 983
AluI AGCT 6 cut(s) 99, 290, 504, 702, 956, 983
Alw26I GTCTC 2 cut(s) 302, 359
AlwI GGATC 2 cut(s) 230, 685
AlwNI CAGNNNCTG 4 cut(s) 290, 293, 421, 565
AoxI GGCC 5 cut(s) 148, 313, 614, 654, 991
ApeKI GCWGC 4 cut(s) 287, 290, 699, 956
ApoI RAATTY 2 cut(s) 199, 358
AspLEI GCGC 2 cut(s) 34, 788
AspS9I GGNCC 4 cut(s) 149, 614, 655, 992
AsuHPI GGTGA 3 cut(s) 157, 721, 806
BanI GGYRCC 1 cut(s) 755
BanII GRGCYC 1 cut(s) 717
BbsI GAAGAC 2 cut(s) 203, 878
BbvI GCAGC 4 cut(s) 277, 299, 711, 943
BccI CCATC 5 cut(s) 110, 388, 756, 767, 1022
BceAI ACGGC 3 cut(s) 145, 422, 787
BciT130I CCWGG 3 cut(s) 422, 663, 753
BcoDI GTCTC 2 cut(s) 302, 359
BfaI CTAG 1 cut(s) 602
BfoI RGCGCY 1 cut(s) 789
BfuAI ACCTGC 1 cut(s) 25
BglI GCCNNNNNGGC 1 cut(s) 156
BisI GCNGC 7 cut(s) 24, 27, 30, 288, 291, 700, 957
BlsI GCNGC 7 cut(s) 25, 28, 31, 289, 292, 701, 958
Bme1390I CCNGG 3 cut(s) 422, 663, 753
BmgT120I GGNCC 4 cut(s) 149, 614, 655, 992
BmiI GGNNCC 4 cut(s) 39, 151, 757, 866
BmrFI CCNGG 3 cut(s) 422, 663, 753
BmrI ACTGGG 1 cut(s) 409
BmsI GCATC 4 cut(s) 64, 141, 653, 925
BmuI ACTGGG 1 cut(s) 409
BpiI GAAGAC 2 cut(s) 203, 878
BpmI CTGGAG 1 cut(s) 207
Bpu10I CCTNAGC 2 cut(s) 263, 341
Bsa29I ATCGAT 1 cut(s) 135
BsaI GGTCTC 1 cut(s) 359
BsaJI CCNNGG 2 cut(s) 694, 868
BsaXI ACNNNNNCTCC 2 cut(s) 499, 529
Bsc4I CCNNNNNNNGG 3 cut(s) 345, 421, 1030
Bse118I RCCGGY 2 cut(s) 315, 345
Bse1I ACTGG 1 cut(s) 415
Bse3DI GCAATG 3 cut(s) 51, 463, 781
BseBI CCWGG 3 cut(s) 422, 663, 753
BseCI ATCGAT 1 cut(s) 135
BseDI CCNNGG 2 cut(s) 694, 868
BseGI GGATG 2 cut(s) 748, 940
BseLI CCNNNNNNNGG 3 cut(s) 345, 421, 1030
BseMI GCAATG 3 cut(s) 51, 463, 781
BseMII CTCAG 2 cut(s) 277, 730
BseNI ACTGG 1 cut(s) 415
BseRI GAGGAG 1 cut(s) 507
BseXI GCAGC 4 cut(s) 277, 299, 711, 943
BseYI CCCAGC 2 cut(s) 205, 657
BsgI GTGCAG 1 cut(s) 798
BshFI GGCC 5 cut(s) 150, 315, 616, 656, 993
BshNI GGYRCC 1 cut(s) 755
BshVI ATCGAT 1 cut(s) 135
BsiSI CCGG 4 cut(s) 41, 156, 316, 346
BslFI GGGAC 1 cut(s) 74
BslI CCNNNNNNNGG 3 cut(s) 345, 421, 1030
BsmAI GTCTC 2 cut(s) 302, 359
BsmFI GGGAC 1 cut(s) 74
BsmI GAATGC 1 cut(s) 627
BsnI GGCC 5 cut(s) 150, 315, 616, 656, 993
Bso31I GGTCTC 1 cut(s) 359
Bsp1286I GDGCHC 1 cut(s) 717
Bsp143I GATC 4 cut(s) 235, 328, 690, 719
BspACI CCGC 4 cut(s) 23, 26, 29, 995
BspANI GGCC 5 cut(s) 150, 315, 616, 656, 993
BspCNI CTCAG 2 cut(s) 276, 729
BspDI ATCGAT 1 cut(s) 135
BspLI GGNNCC 4 cut(s) 39, 151, 757, 866
BspMI ACCTGC 1 cut(s) 25
BspPI GGATC 2 cut(s) 230, 685
BspT107I GGYRCC 1 cut(s) 755
BspTNI GGTCTC 1 cut(s) 359
BsrDI GCAATG 3 cut(s) 51, 463, 781
BsrFI RCCGGY 2 cut(s) 315, 345
BsrI ACTGG 1 cut(s) 415
BssAI RCCGGY 2 cut(s) 315, 345
BssECI CCNNGG 2 cut(s) 694, 868
BssMI GATC 4 cut(s) 235, 328, 690, 719
BssNAI GTATAC 1 cut(s) 336
BssT1I CCWWGG 2 cut(s) 694, 868
Bst1107I GTATAC 1 cut(s) 336
Bst2UI CCWGG 3 cut(s) 422, 663, 753
Bst4CI ACNGT 1 cut(s) 578
BstAPI GCANNNNNTGC 2 cut(s) 50, 971
BstC8I GCNNGC 7 cut(s) 79, 295, 317, 347, 847, 981, 995
BstDEI CTNAG 4 cut(s) 263, 341, 426, 716
BstF5I GGATG 2 cut(s) 748, 940
BstH2I RGCGCY 1 cut(s) 789
BstHHI GCGC 2 cut(s) 34, 788
BstKTI GATC 4 cut(s) 238, 331, 693, 722
BstMAI GTCTC 2 cut(s) 302, 359
BstMBI GATC 4 cut(s) 235, 328, 690, 719
BstMWI GCNNNNNNNGC 9 cut(s) 29, 50, 156, 287, 721, 785, 962, 971, 980
BstNI CCWGG 3 cut(s) 422, 663, 753
BstSCI CCNGG 3 cut(s) 420, 661, 751
BstV1I GCAGC 4 cut(s) 277, 299, 711, 943
BstV2I GAAGAC 2 cut(s) 203, 878
BstX2I RGATCY 2 cut(s) 235, 690
BstYI RGATCY 2 cut(s) 235, 690
BstZ17I GTATAC 1 cut(s) 336
Bsu15I ATCGAT 1 cut(s) 135
BsuRI GGCC 5 cut(s) 150, 315, 616, 656, 993
BsuTUI ATCGAT 1 cut(s) 135
BtgZI GCGATG 1 cut(s) 88
BtsCI GGATG 2 cut(s) 748, 940
BtsIMutI CAGTG 2 cut(s) 24, 835
BveI ACCTGC 1 cut(s) 25
Cac8I GCNNGC 7 cut(s) 79, 295, 317, 347, 847, 981, 995
CaiI CAGNNNCTG 4 cut(s) 290, 293, 421, 565
CfoI GCGC 2 cut(s) 34, 788
Cfr10I RCCGGY 2 cut(s) 315, 345
Cfr13I GGNCC 4 cut(s) 149, 614, 655, 992
ClaI ATCGAT 1 cut(s) 135
Csp6I GTAC 2 cut(s) 418, 769
CviAII CATG 2 cut(s) 481, 903
CviQI GTAC 2 cut(s) 418, 769
DdeI CTNAG 4 cut(s) 263, 341, 426, 716
DpnI GATC 4 cut(s) 237, 330, 692, 721
DpnII GATC 4 cut(s) 235, 328, 690, 719
Eco130I CCWWGG 2 cut(s) 694, 868
Eco24I GRGCYC 1 cut(s) 717
Eco31I GGTCTC 1 cut(s) 359
Eco57I CTGAAG 2 cut(s) 267, 972
EcoO109I RGGNCCY 1 cut(s) 149
EcoRII CCWGG 3 cut(s) 420, 661, 751
EcoT14I CCWWGG 2 cut(s) 694, 868
EcoT38I GRGCYC 1 cut(s) 717
ErhI CCWWGG 2 cut(s) 694, 868
FaeI CATG 2 cut(s) 484, 906
FaqI GGGAC 1 cut(s) 74
FatI CATG 2 cut(s) 480, 902
FauI CCCGC 1 cut(s) 1002
FblI GTMKAC 1 cut(s) 335
Fnu4HI GCNGC 7 cut(s) 24, 27, 30, 288, 291, 700, 957
FokI GGATG 2 cut(s) 735, 947
FriOI GRGCYC 1 cut(s) 717
Fsp4HI GCNGC 7 cut(s) 24, 27, 30, 288, 291, 700, 957
FspBI CTAG 1 cut(s) 602
GlaI GCGC 2 cut(s) 33, 787
GluI GCNGC 7 cut(s) 24, 27, 30, 288, 291, 700, 957
GsaI CCCAGC 2 cut(s) 209, 661
GsuI CTGGAG 1 cut(s) 207
HaeII RGCGCY 1 cut(s) 789
HaeIII GGCC 5 cut(s) 150, 315, 616, 656, 993
HapII CCGG 4 cut(s) 41, 156, 316, 346
HhaI GCGC 2 cut(s) 34, 788
Hin1II CATG 2 cut(s) 484, 906
Hin6I GCGC 2 cut(s) 32, 786
HinP1I GCGC 2 cut(s) 32, 786
HincII GTYRAC 1 cut(s) 676
HindII GTYRAC 1 cut(s) 676
HinfI GANTC 6 cut(s) 14, 62, 162, 270, 376, 562
HpaII CCGG 4 cut(s) 41, 156, 316, 346
HphI GGTGA 3 cut(s) 157, 721, 806
Hpy166II GTNNAC 3 cut(s) 336, 676, 769
Hpy188I TCNGA 4 cut(s) 690, 719, 804, 1003
Hpy188III TCNNGA 1 cut(s) 364
Hpy8I GTNNAC 3 cut(s) 336, 676, 769
HpyAV CCTTC 3 cut(s) 495, 545, 660
HpyCH4III ACNGT 1 cut(s) 578
HpyCH4V TGCA 6 cut(s) 233, 574, 627, 815, 896, 959
HpyF10VI GCNNNNNNNGC 9 cut(s) 29, 50, 156, 287, 721, 785, 962, 971, 980
HpyF3I CTNAG 4 cut(s) 263, 341, 426, 716
Hsp92II CATG 2 cut(s) 484, 906
HspAI GCGC 2 cut(s) 32, 786
KroI GCCGGC 2 cut(s) 315, 345
KroNI GCCGGC 2 cut(s) 317, 347
Kzo9I GATC 4 cut(s) 235, 328, 690, 719
LmnI GCTCC 3 cut(s) 104, 226, 453
Lsp1109I GCAGC 4 cut(s) 277, 299, 711, 943
LweI GCATC 4 cut(s) 64, 141, 653, 925
MaeI CTAG 1 cut(s) 602
MaeIII GTNAC 3 cut(s) 727, 794, 1007
MalI GATC 4 cut(s) 237, 330, 692, 721
MboI GATC 4 cut(s) 235, 328, 690, 719
MboII GAAGA 4 cut(s) 186, 203, 489, 878
MflI RGATCY 2 cut(s) 235, 690
MhlI GDGCHC 1 cut(s) 717
MluCI AATT 6 cut(s) 115, 199, 274, 358, 609, 855
MlyI GAGTC 3 cut(s) 23, 56, 279
MnlI CCTC 8 cut(s) 140, 163, 488, 500, 528, 582, 855, 1009
MroNI GCCGGC 2 cut(s) 315, 345
MseI TTAA 4 cut(s) 383, 543, 738, 987
MslI CAYNNNNRTG 2 cut(s) 753, 764
MspA1I CMGCKG 1 cut(s) 290
MspI CCGG 4 cut(s) 41, 156, 316, 346
MspR9I CCNGG 3 cut(s) 422, 663, 753
Mva1269I GAATGC 1 cut(s) 627
MvaI CCWGG 3 cut(s) 422, 663, 753
MwoI GCNNNNNNNGC 9 cut(s) 29, 50, 156, 287, 721, 785, 962, 971, 980
NaeI GCCGGC 2 cut(s) 317, 347
NdeII GATC 4 cut(s) 235, 328, 690, 719
NgoMIV GCCGGC 2 cut(s) 315, 345
NlaIII CATG 2 cut(s) 484, 906
NlaIV GGNNCC 4 cut(s) 39, 151, 757, 866
NmuCI GTSAC 2 cut(s) 727, 794
PctI GAATGC 1 cut(s) 627
PdiI GCCGGC 2 cut(s) 317, 347
PfeI GAWTC 3 cut(s) 162, 376, 562
PflFI GACNNNGTC 1 cut(s) 680
PflMI CCANNNNNTGG 2 cut(s) 421, 1030
PkrI GCNGC 7 cut(s) 25, 28, 31, 289, 292, 701, 958
PleI GAGTC 3 cut(s) 22, 56, 278
PpsI GAGTC 3 cut(s) 22, 56, 278
Psp6I CCWGG 3 cut(s) 420, 661, 751
PspFI CCCAGC 2 cut(s) 205, 657
PspGI CCWGG 3 cut(s) 420, 661, 751
PspN4I GGNNCC 4 cut(s) 39, 151, 757, 866
PspPI GGNCC 4 cut(s) 149, 614, 655, 992
PstNI CAGNNNCTG 4 cut(s) 290, 293, 421, 565
PsuI RGATCY 2 cut(s) 235, 690
PsyI GACNNNGTC 1 cut(s) 680
PvuII CAGCTG 1 cut(s) 290
RsaI GTAC 2 cut(s) 419, 770
RsaNI GTAC 2 cut(s) 418, 769
RseI CAYNNNNRTG 2 cut(s) 753, 764
SaqAI TTAA 4 cut(s) 383, 543, 738, 987
SatI GCNGC 7 cut(s) 24, 27, 30, 288, 291, 700, 957
Sau3AI GATC 4 cut(s) 235, 328, 690, 719
Sau96I GGNCC 4 cut(s) 149, 614, 655, 992
SchI GAGTC 3 cut(s) 23, 56, 279
ScrFI CCNGG 3 cut(s) 422, 663, 753
SduI GDGCHC 1 cut(s) 717
SfaNI GCATC 4 cut(s) 64, 141, 653, 925
SmiMI CAYNNNNRTG 2 cut(s) 753, 764
Sse9I AATT 6 cut(s) 115, 199, 274, 358, 609, 855
SsiI CCGC 4 cut(s) 23, 26, 29, 995
SspMI CTAG 1 cut(s) 602
StyD4I CCNGG 3 cut(s) 420, 661, 751
StyI CCWWGG 2 cut(s) 694, 868
TaaI ACNGT 1 cut(s) 578
TaqI TCGA 4 cut(s) 135, 303, 363, 431
TasI AATT 6 cut(s) 115, 199, 274, 358, 609, 855
TauI GCSGC 3 cut(s) 26, 29, 32
TfiI GAWTC 3 cut(s) 162, 376, 562
Tru1I TTAA 4 cut(s) 383, 543, 738, 987
Tru9I TTAA 4 cut(s) 383, 543, 738, 987
TscAI CASTG 2 cut(s) 24, 835
TseFI GTSAC 2 cut(s) 727, 794
TseI GCWGC 4 cut(s) 287, 290, 699, 956
Tsp45I GTSAC 2 cut(s) 727, 794
TspDTI ATGAA 1 cut(s) 82
TspRI CASTG 2 cut(s) 24, 835
Tth111I GACNNNGTC 1 cut(s) 680
Van91I CCANNNNNTGG 2 cut(s) 421, 1030
XapI RAATTY 2 cut(s) 199, 358
XcmI CCANNNNNNNNNTGG 1 cut(s) 562
XmiI GTMKAC 1 cut(s) 335
XspI CTAG 1 cut(s) 602
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.