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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 77 |
| AfaI | GTAC | 1 cut(s) | 15 |
| AluBI | AGCT | 1 cut(s) | 185 |
| AluI | AGCT | 1 cut(s) | 185 |
| AoxI | GGCC | 1 cut(s) | 71 |
| ApoI | RAATTY | 1 cut(s) | 77 |
| AspS9I | GGNCC | 1 cut(s) | 72 |
| AsuHPI | GGTGA | 1 cut(s) | 166 |
| BmgT120I | GGNCC | 1 cut(s) | 72 |
| BseRI | GAGGAG | 1 cut(s) | 192 |
| BshFI | GGCC | 1 cut(s) | 73 |
| BsnI | GGCC | 1 cut(s) | 73 |
| BspANI | GGCC | 1 cut(s) | 73 |
| BstDEI | CTNAG | 1 cut(s) | 173 |
| BsuRI | GGCC | 1 cut(s) | 73 |
| Cfr13I | GGNCC | 1 cut(s) | 72 |
| Csp6I | GTAC | 1 cut(s) | 14 |
| CviAII | CATG | 1 cut(s) | 62 |
| CviJI | RGCY | 3 cut(s) | 73, 92, 185 |
| CviKI_1 | RGCY | 3 cut(s) | 73, 92, 185 |
| CviQI | GTAC | 1 cut(s) | 14 |
| DdeI | CTNAG | 1 cut(s) | 173 |
| EcoRI | GAATTC | 1 cut(s) | 77 |
| FaeI | CATG | 1 cut(s) | 65 |
| FaiI | YATR | 5 cut(s) | 63, 136, 138, 143, 151 |
| FatI | CATG | 1 cut(s) | 61 |
| HaeIII | GGCC | 1 cut(s) | 73 |
| Hin1II | CATG | 1 cut(s) | 65 |
| HinfI | GANTC | 2 cut(s) | 38, 169 |
| HphI | GGTGA | 1 cut(s) | 166 |
| Hpy188I | TCNGA | 2 cut(s) | 35, 43 |
| Hpy99I | CGWCG | 1 cut(s) | 102 |
| HpyF3I | CTNAG | 1 cut(s) | 173 |
| Hsp92II | CATG | 1 cut(s) | 65 |
| MluCI | AATT | 2 cut(s) | 77, 159 |
| MnlI | CCTC | 2 cut(s) | 63, 170 |
| MseI | TTAA | 1 cut(s) | 107 |
| NlaIII | CATG | 1 cut(s) | 65 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 31 |
| PfeI | GAWTC | 2 cut(s) | 38, 169 |
| PspPI | GGNCC | 1 cut(s) | 72 |
| RsaI | GTAC | 1 cut(s) | 15 |
| RsaNI | GTAC | 1 cut(s) | 14 |
| SaqAI | TTAA | 1 cut(s) | 107 |
| Sau96I | GGNCC | 1 cut(s) | 72 |
| SetI | ASST | 2 cut(s) | 25, 187 |
| SgeI | CNNG | 7 cut(s) | 32, 38, 47, 74, 86, 95, 113 |
| Sse9I | AATT | 2 cut(s) | 77, 159 |
| TaqI | TCGA | 1 cut(s) | 6 |
| TasI | AATT | 2 cut(s) | 77, 159 |
| TatI | WGTACW | 1 cut(s) | 13 |
| TfiI | GAWTC | 2 cut(s) | 38, 169 |
| Tru1I | TTAA | 1 cut(s) | 107 |
| Tru9I | TTAA | 1 cut(s) | 107 |
| TspDTI | ATGAA | 1 cut(s) | 114 |
| TspGWI | ACGGA | 1 cut(s) | 68 |
| XapI | RAATTY | 1 cut(s) | 77 |