Rmu_sc0002039.1_g000005

Plant invertase/pectin methylesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002039.1
OG0001702
Physical Location & Seq
Forward (+)
23960 .. 25551
1592 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 681 bp
atgagtgatgatttcatcgaacaatcttgcaagatagctgctggtcgtgaaccatctttgaactacaccttctgtgtctcaactcttaaggccagccctaaaagccatggtgcagaccttgatggactcatgtgcatcgtacttcgccaagccatagacaaggtggcctacatcaatgacctcatccaaaaaaaatgtgccgatgaatcccacgtctttagctacgacctatgcacaacctctcttcaagcagttccagtcagccatgtggctaatctccaaggactagcattgatagcaatggagctagcactaaacaatgcaagcagcacgcttcagatcataaaaaatttgttgatgagcaacgaaagtctagatccttttgttgtggtttgcttgaaggactgcttgcagctctattcagacgggatcacaacactggaggatgctgaaggagcatttctgggtgagcattatgacaacgccaatatttgtcttagtgcagttatggaagcatcgagtacatgtgaggaaggctttaaggagaaggaaggtgaagtattaccgtctccattgacaaatgagaattacaatctttttcaattatgtgatattgcactgtgcataggccacttgcttacctcgggttctaccttgttgatgaggtcctctgcaacttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

226

Amino Acids

24.46

Weight (kDa)

4.48

Isoelectric Point (pI)

48.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 60 - 209 3.7e-26 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 371, 437
AcsI RAATTY 1 cut(s) 349
AcuI CTGAAG 2 cut(s) 320, 471
AfaI GTAC 2 cut(s) 141, 523
AflII CTTAAG 1 cut(s) 86
AflIII ACRYGT 1 cut(s) 524
AgsI TTSAA 4 cut(s) 61, 248, 400, 602
AjiI CACGTC 1 cut(s) 214
AluBI AGCT 4 cut(s) 38, 222, 307, 415
AluI AGCT 4 cut(s) 38, 222, 307, 415
Alw26I GTCTC 2 cut(s) 82, 573
AlwI GGATC 2 cut(s) 371, 437
Ama87I CYCGRG 1 cut(s) 643
AoxI GGCC 3 cut(s) 90, 165, 628
ApeKI GCWGC 3 cut(s) 38, 327, 412
ApoI RAATTY 1 cut(s) 349
AspS9I GGNCC 1 cut(s) 666
AsuHPI GGTGA 2 cut(s) 479, 566
AsuNHI GCTAGC 1 cut(s) 307
AvaI CYCGRG 1 cut(s) 643
AvaII GGWCC 1 cut(s) 666
BbvI GCAGC 3 cut(s) 25, 339, 424
BccI CCATC 2 cut(s) 61, 116
BcoDI GTCTC 2 cut(s) 82, 573
BfaI CTAG 3 cut(s) 287, 308, 374
BfrI CTTAAG 1 cut(s) 86
BisI GCNGC 3 cut(s) 39, 328, 413
BlsI GCNGC 3 cut(s) 40, 329, 414
Bme18I GGWCC 1 cut(s) 666
BmeT110I CYCGRG 1 cut(s) 643
BmgBI CACGTC 1 cut(s) 214
BmgT120I GGNCC 1 cut(s) 666
BmsI GCATC 3 cut(s) 144, 436, 524
BmtI GCTAGC 1 cut(s) 311
BpmI CTGGAG 1 cut(s) 461
BsaJI CCNNGG 3 cut(s) 106, 280, 642
Bse1I ACTGG 2 cut(s) 257, 444
Bse3DI GCAATG 1 cut(s) 306
BseDI CCNNGG 3 cut(s) 106, 280, 642
BseGI GGATG 2 cut(s) 183, 451
BseMI GCAATG 1 cut(s) 306
BseNI ACTGG 2 cut(s) 257, 444
BseXI GCAGC 3 cut(s) 25, 339, 424
BsgI GTGCAG 2 cut(s) 132, 522
BshFI GGCC 3 cut(s) 92, 167, 630
BsiHKCI CYCGRG 1 cut(s) 643
BsmAI GTCTC 2 cut(s) 82, 573
BsmBI CGTCTC 1 cut(s) 573
BsnI GGCC 3 cut(s) 92, 167, 630
BsoBI CYCGRG 1 cut(s) 643
Bsp143I GATC 3 cut(s) 339, 376, 429
Bsp19I CCATGG 1 cut(s) 106
BspANI GGCC 3 cut(s) 92, 167, 630
BspOI GCTAGC 1 cut(s) 311
BspPI GGATC 2 cut(s) 371, 437
BspTI CTTAAG 1 cut(s) 86
BsrDI GCAATG 1 cut(s) 306
BsrI ACTGG 2 cut(s) 257, 444
BssECI CCNNGG 3 cut(s) 106, 280, 642
BssMI GATC 3 cut(s) 339, 376, 429
BssT1I CCWWGG 2 cut(s) 106, 280
Bst4CI ACNGT 2 cut(s) 567, 621
Bst6I CTCTTC 1 cut(s) 249
BstAFI CTTAAG 1 cut(s) 86
BstC8I GCNNGC 5 cut(s) 94, 309, 325, 332, 410
BstDEI CTNAG 1 cut(s) 497
BstDSI CCRYGG 1 cut(s) 106
BstF5I GGATG 2 cut(s) 183, 451
BstKTI GATC 3 cut(s) 342, 379, 432
BstMAI GTCTC 2 cut(s) 82, 573
BstMBI GATC 3 cut(s) 339, 376, 429
BstMWI GCNNNNNNNGC 3 cut(s) 102, 296, 455
BstNSI RCATGY 1 cut(s) 528
BstV1I GCAGC 3 cut(s) 25, 339, 424
BstX2I RGATCY 1 cut(s) 376
BstYI RGATCY 1 cut(s) 376
BsuRI GGCC 3 cut(s) 92, 167, 630
BtgI CCRYGG 1 cut(s) 106
BtrI CACGTC 1 cut(s) 214
BtsCI GGATG 2 cut(s) 183, 451
BtsIMutI CAGTG 2 cut(s) 437, 617
Cac8I GCNNGC 5 cut(s) 94, 309, 325, 332, 410
Cfr13I GGNCC 1 cut(s) 666
Csp6I GTAC 2 cut(s) 140, 522
CviAII CATG 4 cut(s) 107, 130, 266, 525
CviQI GTAC 2 cut(s) 140, 522
DdeI CTNAG 1 cut(s) 497
DpnI GATC 3 cut(s) 341, 378, 431
DpnII GATC 3 cut(s) 339, 376, 429
Eam1104I CTCTTC 1 cut(s) 249
EarI CTCTTC 1 cut(s) 249
Eco130I CCWWGG 2 cut(s) 106, 280
Eco47I GGWCC 1 cut(s) 666
Eco57I CTGAAG 2 cut(s) 320, 471
Eco88I CYCGRG 1 cut(s) 643
EcoO109I RGGNCCY 1 cut(s) 666
EcoT14I CCWWGG 2 cut(s) 106, 280
ErhI CCWWGG 2 cut(s) 106, 280
Esp3I CGTCTC 1 cut(s) 573
FaeI CATG 4 cut(s) 110, 133, 269, 528
FalI AAGNNNNNCTT 2 cut(s) 392, 424
FatI CATG 4 cut(s) 106, 129, 265, 524
Fnu4HI GCNGC 3 cut(s) 39, 328, 413
FokI GGATG 2 cut(s) 170, 458
Fsp4HI GCNGC 3 cut(s) 39, 328, 413
FspBI CTAG 3 cut(s) 287, 308, 374
GluI GCNGC 3 cut(s) 39, 328, 413
GsuI CTGGAG 1 cut(s) 461
HaeIII GGCC 3 cut(s) 92, 167, 630
Hin1II CATG 4 cut(s) 110, 133, 269, 528
HinfI GANTC 2 cut(s) 126, 206
HphI GGTGA 2 cut(s) 479, 566
Hpy166II GTNNAC 1 cut(s) 50
Hpy188I TCNGA 2 cut(s) 339, 424
Hpy188III TCNNGA 2 cut(s) 47, 374
Hpy8I GTNNAC 1 cut(s) 50
HpyAV CCTTC 6 cut(s) 79, 394, 446, 527, 541, 545
HpyCH4III ACNGT 2 cut(s) 567, 621
HpyCH4IV ACGT 1 cut(s) 213
HpyF10VI GCNNNNNNNGC 3 cut(s) 102, 296, 455
HpyF3I CTNAG 1 cut(s) 497
HpySE526I ACGT 1 cut(s) 213
Hsp92II CATG 4 cut(s) 110, 133, 269, 528
Kzo9I GATC 3 cut(s) 339, 376, 429
LmnI GCTCC 2 cut(s) 304, 455
LpnPI CCDG 5 cut(s) 27, 106, 270, 425, 449
Lsp1109I GCAGC 3 cut(s) 25, 339, 424
LweI GCATC 3 cut(s) 144, 436, 524
MaeI CTAG 3 cut(s) 287, 308, 374
MaeII ACGT 1 cut(s) 213
MalI GATC 3 cut(s) 341, 378, 431
MboI GATC 3 cut(s) 339, 376, 429
MboII GAAGA 1 cut(s) 236
MflI RGATCY 1 cut(s) 376
MluCI AATT 3 cut(s) 349, 586, 602
MlyI GAGTC 1 cut(s) 120
MnlI CCTC 7 cut(s) 191, 250, 436, 523, 652, 657, 679
MseI TTAA 3 cut(s) 87, 540, 679
MspCI CTTAAG 1 cut(s) 86
MwoI GCNNNNNNNGC 3 cut(s) 102, 296, 455
NcoI CCATGG 1 cut(s) 106
NdeII GATC 3 cut(s) 339, 376, 429
NheI GCTAGC 1 cut(s) 307
NlaIII CATG 4 cut(s) 110, 133, 269, 528
NspI RCATGY 1 cut(s) 528
PciI ACATGT 1 cut(s) 524
PfeI GAWTC 1 cut(s) 206
PkrI GCNGC 3 cut(s) 40, 329, 414
PleI GAGTC 1 cut(s) 120
PpsI GAGTC 1 cut(s) 120
PpuMI RGGWCCY 1 cut(s) 666
PscI ACATGT 1 cut(s) 524
Psp5II RGGWCCY 1 cut(s) 666
PspPI GGNCC 1 cut(s) 666
PspPPI RGGWCCY 1 cut(s) 666
PsuI RGATCY 1 cut(s) 376
RsaI GTAC 2 cut(s) 141, 523
RsaNI GTAC 2 cut(s) 140, 522
SaqAI TTAA 3 cut(s) 87, 540, 679
SatI GCNGC 3 cut(s) 39, 328, 413
Sau3AI GATC 3 cut(s) 339, 376, 429
Sau96I GGNCC 1 cut(s) 666
SchI GAGTC 1 cut(s) 120
SfaNI GCATC 3 cut(s) 144, 436, 524
SinI GGWCC 1 cut(s) 666
SmlI CTYRAG 1 cut(s) 86
SmoI CTYRAG 1 cut(s) 86
Sse9I AATT 3 cut(s) 349, 586, 602
SspI AATATT 1 cut(s) 490
SspMI CTAG 3 cut(s) 287, 308, 374
StyI CCWWGG 2 cut(s) 106, 280
TaaI ACNGT 2 cut(s) 567, 621
TaiI ACGT 1 cut(s) 216
TaqI TCGA 2 cut(s) 18, 518
TasI AATT 3 cut(s) 349, 586, 602
TatI WGTACW 1 cut(s) 521
TfiI GAWTC 1 cut(s) 206
Tru1I TTAA 3 cut(s) 87, 540, 679
Tru9I TTAA 3 cut(s) 87, 540, 679
TscAI CASTG 2 cut(s) 444, 624
TseI GCWGC 3 cut(s) 38, 327, 412
TspDTI ATGAA 2 cut(s) 4, 219
TspRI CASTG 2 cut(s) 444, 624
Vha464I CTTAAG 1 cut(s) 86
VpaK11BI GGWCC 1 cut(s) 666
XapI RAATTY 1 cut(s) 349
XbaI TCTAGA 1 cut(s) 373
XceI RCATGY 1 cut(s) 528
XcmI CCANNNNNNNNNTGG 1 cut(s) 160
XspI CTAG 3 cut(s) 287, 308, 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.