Rmu_sc0002724.1_g000006

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002724.1
OG0004212
Physical Location & Seq
Reverse (-)
48878 .. 49606
729 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002724.1_g000006.1.cds

Sequence Viewer

Length: 591 bp
atggcagagaggattcgtgagttggactcccaggtgcttgcaaccaacaaggagctcagggtgaccaagactcagctgggtcaaaagaagaaagatgtggctaaaatggaggattatcacaaaaggctgaaggaaaaagaaatagaggttgatcagctgggagaaaaggttaggggcctggagaaggaacttagggttaaggccaaagagtgtaagagaaacaaggatttggaggtggaagtccaagaccttagagagagggttgagggtctcaggagcaaggccaaggcagtttctttgggtgttgatgttgcgcctccagaaatcaagggtagcccctccaaggctacacttgctaatgtaagtccaggtcttgccctgtctggctatgaagttgagggattggacagtggaggagtcgaccaggcaagtgggtcagtattgggagacgaacctaccgaggcactgagcgacgaccgccagcaaggtaagtctagcactggtgacactccagctgaggacaaaggaggtgttcttggagacggggaagatggagatgaaagggccaaggtaacaaataccgatccttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

196

Amino Acids

21.2

Weight (kDa)

5.06

Isoelectric Point (pI)

42.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 420
AciI CCGC 1 cut(s) 478
AclWI GGATC 1 cut(s) 578
AcuI CTGAAG 1 cut(s) 149
AfiI CCNNNNNNNGG 2 cut(s) 184, 343
AjnI CCWGG 4 cut(s) 30, 177, 367, 423
AluBI AGCT 4 cut(s) 55, 76, 157, 515
AluI AGCT 4 cut(s) 55, 76, 157, 515
Alw21I GWGCWC 1 cut(s) 57
Alw26I GTCTC 3 cut(s) 275, 441, 534
AlwI GGATC 1 cut(s) 578
AoxI GGCC 4 cut(s) 175, 201, 282, 564
AspLEI GCGC 1 cut(s) 316
AspS9I GGNCC 2 cut(s) 175, 564
AsuHPI GGTGA 2 cut(s) 73, 515
BanII GRGCYC 1 cut(s) 57
Bbv12I GWGCWC 1 cut(s) 57
BbvCI CCTCAGC 1 cut(s) 516
BccI CCATC 1 cut(s) 545
BciT130I CCWGG 4 cut(s) 32, 179, 369, 425
BclI TGATCA 1 cut(s) 151
BcoDI GTCTC 3 cut(s) 275, 441, 534
BfaI CTAG 1 cut(s) 495
Bme1390I CCNGG 4 cut(s) 32, 179, 369, 425
BmgT120I GGNCC 2 cut(s) 175, 564
BmiI GGNNCC 1 cut(s) 176
BmrFI CCNGG 4 cut(s) 32, 179, 369, 425
BplI GAGNNNNNCTC 2 cut(s) 11, 43
BpmI CTGGAG 3 cut(s) 200, 303, 495
Bpu10I CCTNAGC 2 cut(s) 56, 516
BsaI GGTCTC 1 cut(s) 275
BsaJI CCNNGG 5 cut(s) 30, 285, 342, 459, 567
BsaXI ACNNNNNCTCC 2 cut(s) 153, 183
Bsc4I CCNNNNNNNGG 2 cut(s) 184, 343
Bse1I ACTGG 1 cut(s) 505
BseBI CCWGG 4 cut(s) 32, 179, 369, 425
BseDI CCNNGG 5 cut(s) 30, 285, 342, 459, 567
BseLI CCNNNNNNNGG 2 cut(s) 184, 343
BseMII CTCAG 5 cut(s) 70, 86, 286, 458, 507
BseNI ACTGG 1 cut(s) 505
BseRI GAGGAG 1 cut(s) 429
BseYI CCCAGC 2 cut(s) 76, 157
Bsh1285I CGRYCG 1 cut(s) 478
BshFI GGCC 4 cut(s) 177, 203, 284, 566
BsiEI CGRYCG 1 cut(s) 478
BsiHKAI GWGCWC 1 cut(s) 57
BslI CCNNNNNNNGG 2 cut(s) 184, 343
BsmAI GTCTC 3 cut(s) 275, 441, 534
BsmBI CGTCTC 2 cut(s) 441, 534
BsnI GGCC 4 cut(s) 177, 203, 284, 566
Bso31I GGTCTC 1 cut(s) 275
Bsp1286I GDGCHC 1 cut(s) 57
Bsp143I GATC 2 cut(s) 151, 583
BspACI CCGC 1 cut(s) 478
BspANI GGCC 4 cut(s) 177, 203, 284, 566
BspCNI CTCAG 5 cut(s) 69, 85, 285, 459, 508
BspLI GGNNCC 1 cut(s) 176
BspPI GGATC 1 cut(s) 578
BspTNI GGTCTC 1 cut(s) 275
BsrI ACTGG 1 cut(s) 505
BssECI CCNNGG 5 cut(s) 30, 285, 342, 459, 567
BssMI GATC 2 cut(s) 151, 583
BssT1I CCWWGG 3 cut(s) 285, 342, 567
Bst2UI CCWGG 4 cut(s) 32, 179, 369, 425
Bst4CI ACNGT 1 cut(s) 410
BstC8I GCNNGC 2 cut(s) 39, 482
BstDEI CTNAG 7 cut(s) 56, 72, 191, 251, 272, 467, 516
BstEII GGTNACC 1 cut(s) 61
BstENI CCTNNNNNAGG 1 cut(s) 182
BstHHI GCGC 1 cut(s) 316
BstKTI GATC 2 cut(s) 154, 586
BstMAI GTCTC 3 cut(s) 275, 441, 534
BstMBI GATC 2 cut(s) 151, 583
BstMCI CGRYCG 1 cut(s) 478
BstMWI GCNNNNNNNGC 2 cut(s) 353, 477
BstNI CCWGG 4 cut(s) 32, 179, 369, 425
BstPI GGTNACC 1 cut(s) 61
BstSCI CCNGG 4 cut(s) 30, 177, 367, 423
BstXI CCANNNNNNTGG 1 cut(s) 431
BsuRI GGCC 4 cut(s) 177, 203, 284, 566
BtsIMutI CAGTG 3 cut(s) 415, 464, 498
Cac8I GCNNGC 2 cut(s) 39, 482
CfoI GCGC 1 cut(s) 316
Cfr13I GGNCC 2 cut(s) 175, 564
DdeI CTNAG 7 cut(s) 56, 72, 191, 251, 272, 467, 516
DpnI GATC 2 cut(s) 153, 585
DpnII GATC 2 cut(s) 151, 583
Ecl136II GAGCTC 1 cut(s) 55
Eco130I CCWWGG 3 cut(s) 285, 342, 567
Eco24I GRGCYC 1 cut(s) 57
Eco31I GGTCTC 1 cut(s) 275
Eco53kI GAGCTC 1 cut(s) 55
Eco57I CTGAAG 1 cut(s) 149
Eco91I GGTNACC 1 cut(s) 61
EcoICRI GAGCTC 1 cut(s) 55
EcoNI CCTNNNNNAGG 1 cut(s) 182
EcoO109I RGGNCCY 1 cut(s) 175
EcoO65I GGTNACC 1 cut(s) 61
EcoRII CCWGG 4 cut(s) 30, 177, 367, 423
EcoT14I CCWWGG 3 cut(s) 285, 342, 567
EcoT38I GRGCYC 1 cut(s) 57
ErhI CCWWGG 3 cut(s) 285, 342, 567
Esp3I CGTCTC 2 cut(s) 441, 534
FaiI YATR 1 cut(s) 390
FbaI TGATCA 1 cut(s) 151
FblI GTMKAC 1 cut(s) 420
FriOI GRGCYC 1 cut(s) 57
FspBI CTAG 1 cut(s) 495
GlaI GCGC 1 cut(s) 315
GsaI CCCAGC 2 cut(s) 80, 161
GsuI CTGGAG 3 cut(s) 200, 303, 495
HaeIII GGCC 4 cut(s) 177, 203, 284, 566
HhaI GCGC 1 cut(s) 316
Hin6I GCGC 1 cut(s) 314
HinP1I GCGC 1 cut(s) 314
HincII GTYRAC 1 cut(s) 421
HindII GTYRAC 1 cut(s) 421
HinfI GANTC 4 cut(s) 13, 26, 70, 417
HphI GGTGA 2 cut(s) 73, 515
Hpy166II GTNNAC 1 cut(s) 421
Hpy188III TCNNGA 3 cut(s) 17, 274, 320
Hpy8I GTNNAC 1 cut(s) 421
Hpy99I CGWCG 1 cut(s) 476
HpyAV CCTTC 2 cut(s) 124, 178
HpyCH4III ACNGT 1 cut(s) 410
HpyCH4V TGCA 1 cut(s) 41
HpyF10VI GCNNNNNNNGC 2 cut(s) 353, 477
HpyF3I CTNAG 7 cut(s) 56, 72, 191, 251, 272, 467, 516
HspAI GCGC 1 cut(s) 314
Ksp22I TGATCA 1 cut(s) 151
Kzo9I GATC 2 cut(s) 151, 583
LmnI GCTCC 2 cut(s) 52, 276
MaeI CTAG 1 cut(s) 495
MaeIII GTNAC 3 cut(s) 61, 503, 571
MalI GATC 2 cut(s) 153, 585
MboI GATC 2 cut(s) 151, 583
MboII GAAGA 2 cut(s) 100, 560
MhlI GDGCHC 1 cut(s) 57
MlyI GAGTC 3 cut(s) 20, 64, 426
MseI TTAA 1 cut(s) 198
MspA1I CMGCKG 3 cut(s) 76, 157, 515
MspR9I CCNGG 4 cut(s) 32, 179, 369, 425
MvaI CCWGG 4 cut(s) 32, 179, 369, 425
MwoI GCNNNNNNNGC 2 cut(s) 353, 477
NdeII GATC 2 cut(s) 151, 583
NlaIV GGNNCC 1 cut(s) 176
NmuCI GTSAC 2 cut(s) 61, 503
PcsI WCGNNNNNNNCGW 1 cut(s) 456
PfeI GAWTC 1 cut(s) 13
PleI GAGTC 3 cut(s) 20, 64, 425
PpsI GAGTC 3 cut(s) 20, 64, 425
Psp124BI GAGCTC 1 cut(s) 57
Psp6I CCWGG 4 cut(s) 30, 177, 367, 423
PspEI GGTNACC 1 cut(s) 61
PspFI CCCAGC 2 cut(s) 76, 157
PspGI CCWGG 4 cut(s) 30, 177, 367, 423
PspN4I GGNNCC 1 cut(s) 176
PspPI GGNCC 2 cut(s) 175, 564
PvuII CAGCTG 3 cut(s) 76, 157, 515
SacI GAGCTC 1 cut(s) 57
SalI GTCGAC 1 cut(s) 419
SaqAI TTAA 1 cut(s) 198
Sau3AI GATC 2 cut(s) 151, 583
Sau96I GGNCC 2 cut(s) 175, 564
SchI GAGTC 3 cut(s) 20, 64, 426
ScrFI CCNGG 4 cut(s) 32, 179, 369, 425
SduI GDGCHC 1 cut(s) 57
SsiI CCGC 1 cut(s) 478
SspMI CTAG 1 cut(s) 495
SstI GAGCTC 1 cut(s) 57
StyD4I CCNGG 4 cut(s) 30, 177, 367, 423
StyI CCWWGG 3 cut(s) 285, 342, 567
TaaI ACNGT 1 cut(s) 410
TaqI TCGA 1 cut(s) 420
TfiI GAWTC 1 cut(s) 13
Tru1I TTAA 1 cut(s) 198
Tru9I TTAA 1 cut(s) 198
TscAI CASTG 3 cut(s) 415, 471, 505
TseFI GTSAC 2 cut(s) 61, 503
Tsp45I GTSAC 2 cut(s) 61, 503
TspDTI ATGAA 2 cut(s) 405, 573
TspRI CASTG 3 cut(s) 415, 471, 505
XagI CCTNNNNNAGG 1 cut(s) 182
XcmI CCANNNNNNNNNTGG 1 cut(s) 73
XmiI GTMKAC 1 cut(s) 420
XspI CTAG 1 cut(s) 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.