Rmu_sc0005712.1_g000017

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005712.1
OG0003970
Physical Location & Seq
Forward (+)
94322 .. 99668
5347 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005712.1_g000017.1.cds

Sequence Viewer

Length: 1191 bp
atgacgtttgtcgttactcggtttgatggattaggtcatctaatgcgaaccccacccgatcttgccgatttcaccattccaccttccgtagccggcttggcacagctgaaagaagagtatgtgtacgaagttggaaaacacaagtggtaccgtgtcgacgaacagttgggacgtcgaaatcttcaactcgtgatctttcactccacactcaaaatcatcgtgcaaggaggatatgaggatgatcagggggaggaggagatcacgaaaatgggaaggatcggcctatgcatcgccggcgtcgaggttgtccggccaggtcgggtttcgcacggctgggtgtcttcgatccagctctgtgggcggcggcggggcaaggcgacaccacagggcgactgcaaggcttgcggcgacttcgggaggtgccgggtccggggccggaggaggccggaggggggagaattccccgggtcgggtcaagcgcttgagacacaccgtcatatgttgtggaaggttcagctggaagcttttggactatcgtcagtgcagtttactggtgggtggtcgaggtttgccctggaaaccgagttgaattcttctcactctctgaagttgcggttgctagatggtggattaatttcggctgtcatattccatcgtaggaagggtcatttggtgtcccctggattcagtaatacaaaccaggcgagcatcatccctgttccttgctcaattggctctacctctcattctgttctttcccatcccggcccttccaattctcctggaaccccctccaccatagccactaactatccagctgtaggagccgtcggtgttccggagcatgcggaaccttatgtagttggacgcgtttggcaaatgcaagttcatgaccatatcatgcagcacctccaatggtatccggcatgcaccattatggtatacgattcattcgcatccttgcctcagagttttgtggaggcagaaaacattccactctgcaaagatgttttccttttccaaagtttgactagagcggccatgctaataaagttggttcctcattgcaataccctctttatgaccgaagggtatccaacattcaagaaacattttaagttgccaactgaatctgaactgtccgtgcgtatggttgggaagacccattttgttttcaaccgtccagtctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

396

Amino Acids

44.0

Weight (kDa)

9.02

Isoelectric Point (pI)

44.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 175
Acc65I GGTACC 1 cut(s) 147
AccB1I GGYRCC 2 cut(s) 147, 420
AccBSI CCGCTC 1 cut(s) 1037
AccI GTMKAC 2 cut(s) 156, 942
AccII CGCG 1 cut(s) 870
AccIII TCCGGA 1 cut(s) 838
AciI CCGC 7 cut(s) 361, 364, 367, 405, 613, 848, 1037
AclWI GGATC 2 cut(s) 284, 340
AcoI YGGCCR 2 cut(s) 311, 1038
AcsI RAATTY 2 cut(s) 458, 589
AcuI CTGAAG 1 cut(s) 626
AcyI GRCGYC 2 cut(s) 172, 297
AfaI GTAC 2 cut(s) 125, 149
AfeI AGCGCT 1 cut(s) 480
AfiI CCNNNNNNNGG 5 cut(s) 430, 452, 469, 470, 821
AflIII ACRYGT 1 cut(s) 868
AgsI TTSAA 4 cut(s) 185, 589, 1105, 1177
AhdI GACNNNNNGTC 1 cut(s) 492
AjnI CCWGG 5 cut(s) 313, 573, 679, 699, 781
AjuI GAANNNNNNNTTGG 2 cut(s) 653, 685
AluBI AGCT 5 cut(s) 106, 352, 517, 524, 818
AluI AGCT 5 cut(s) 106, 352, 517, 524, 818
Alw26I GTCTC 1 cut(s) 479
AlwI GGATC 2 cut(s) 284, 340
Ama87I CYCGRG 1 cut(s) 464
Aor13HI TCCGGA 1 cut(s) 838
Aor51HI AGCGCT 1 cut(s) 480
AoxI GGCC 6 cut(s) 280, 311, 433, 443, 766, 1038
ApeKI GCWGC 1 cut(s) 904
ApoI RAATTY 2 cut(s) 458, 589
AseI ATTAAT 1 cut(s) 632
Asp700I GAANNNNTTC 1 cut(s) 990
Asp718I GGTACC 1 cut(s) 147
AspLEI GCGC 1 cut(s) 481
AspS9I GGNCC 3 cut(s) 427, 433, 767
AsuC2I CCSGG 5 cut(s) 425, 431, 465, 466, 765
AsuHPI GGTGA 1 cut(s) 64
AvaI CYCGRG 1 cut(s) 464
AvaII GGWCC 1 cut(s) 427
BanI GGYRCC 2 cut(s) 147, 420
BauI CACGAG 1 cut(s) 188
BbsI GAAGAC 2 cut(s) 333, 1166
BbvI GCAGC 1 cut(s) 916
BccI CCATC 4 cut(s) 20, 617, 660, 768
BceAI ACGGC 2 cut(s) 346, 812
BciT130I CCWGG 5 cut(s) 315, 575, 681, 701, 783
BciVI GTATCC 2 cut(s) 930, 1104
BclI TGATCA 1 cut(s) 241
BcnI CCSGG 5 cut(s) 425, 431, 465, 466, 765
BcoDI GTCTC 1 cut(s) 479
BfaI CTAG 2 cut(s) 620, 1032
BfmI CTRYAG 1 cut(s) 819
BfoI RGCGCY 1 cut(s) 482
BfuI GTATCC 2 cut(s) 930, 1104
BglI GCCNNNNNGGC 1 cut(s) 98
BisI GCNGC 5 cut(s) 362, 365, 406, 905, 1038
BlsI GCNGC 5 cut(s) 363, 366, 407, 906, 1039
Bme18I GGWCC 1 cut(s) 427
BmeRI GACNNNNNGTC 1 cut(s) 492
BmeT110I CYCGRG 1 cut(s) 464
BmgT120I GGNCC 3 cut(s) 427, 433, 767
BmiI GGNNCC 8 cut(s) 149, 422, 428, 434, 787, 826, 852, 1059
BmsI GCATC 3 cut(s) 297, 717, 965
BoxI GACNNNNGTC 2 cut(s) 8, 535
BpiI GAAGAC 2 cut(s) 333, 1166
BpuEI CTTGAG 1 cut(s) 503
BpuMI CCSGG 5 cut(s) 425, 431, 465, 466, 765
BsaHI GRCGYC 2 cut(s) 172, 297
BsaJI CCNNGG 5 cut(s) 430, 463, 464, 573, 679
BsaWI WCCGGW 1 cut(s) 838
Bsc4I CCNNNNNNNGG 5 cut(s) 430, 452, 469, 470, 821
Bse118I RCCGGY 2 cut(s) 92, 293
Bse1I ACTGG 2 cut(s) 556, 1184
Bse3DI GCAATG 1 cut(s) 1063
BseAI TCCGGA 1 cut(s) 838
BseBI CCWGG 5 cut(s) 315, 575, 681, 701, 783
BseDI CCNNGG 5 cut(s) 430, 463, 464, 573, 679
BseGI GGATG 4 cut(s) 244, 711, 760, 956
BseLI CCNNNNNNNGG 5 cut(s) 430, 452, 469, 470, 821
BseMI GCAATG 1 cut(s) 1063
BseMII CTCAG 1 cut(s) 980
BseNI ACTGG 2 cut(s) 556, 1184
BseRI GAGGAG 3 cut(s) 266, 269, 454
BseXI GCAGC 1 cut(s) 916
BseYI CCCAGC 1 cut(s) 333
BsgI GTGCAG 1 cut(s) 563
Bsh1236I CGCG 1 cut(s) 870
BshFI GGCC 6 cut(s) 282, 313, 435, 445, 768, 1040
BshNI GGYRCC 2 cut(s) 147, 420
BsiHKCI CYCGRG 1 cut(s) 464
BslFI GGGAC 2 cut(s) 183, 661
BslI CCNNNNNNNGG 5 cut(s) 430, 452, 469, 470, 821
BsmAI GTCTC 1 cut(s) 479
BsmFI GGGAC 2 cut(s) 183, 661
BsnI GGCC 6 cut(s) 282, 313, 435, 445, 768, 1040
BsoBI CYCGRG 1 cut(s) 464
Bsp13I TCCGGA 1 cut(s) 838
Bsp143I GATC 6 cut(s) 58, 192, 241, 258, 276, 345
BspACI CCGC 7 cut(s) 361, 364, 367, 405, 613, 848, 1037
BspANI GGCC 6 cut(s) 282, 313, 435, 445, 768, 1040
BspCNI CTCAG 1 cut(s) 979
BspEI TCCGGA 1 cut(s) 838
BspFNI CGCG 1 cut(s) 870
BspHI TCATGA 1 cut(s) 889
BspLI GGNNCC 8 cut(s) 149, 422, 428, 434, 787, 826, 852, 1059
BspPI GGATC 2 cut(s) 284, 340
BspT107I GGYRCC 2 cut(s) 147, 420
BsrBI CCGCTC 1 cut(s) 1037
BsrDI GCAATG 1 cut(s) 1063
BsrFI RCCGGY 2 cut(s) 92, 293
BsrI ACTGG 2 cut(s) 556, 1184
BssAI RCCGGY 2 cut(s) 92, 293
BssECI CCNNGG 5 cut(s) 430, 463, 464, 573, 679
BssMI GATC 6 cut(s) 58, 192, 241, 258, 276, 345
BssNAI GTATAC 1 cut(s) 943
BssNI GRCGYC 2 cut(s) 172, 297
BssSI CACGAG 1 cut(s) 188
Bst1107I GTATAC 1 cut(s) 943
Bst2BI CACGAG 1 cut(s) 188
Bst2UI CCWGG 5 cut(s) 315, 575, 681, 701, 783
Bst4CI ACNGT 5 cut(s) 152, 165, 494, 1140, 1181
Bst6I CTCTTC 1 cut(s) 108
BstACI GRCGYC 2 cut(s) 172, 297
BstAPI GCANNNNNTGC 1 cut(s) 402
BstC8I GCNNGC 6 cut(s) 94, 295, 403, 706, 846, 928
BstDEI CTNAG 1 cut(s) 966
BstF5I GGATG 4 cut(s) 244, 711, 760, 956
BstFNI CGCG 1 cut(s) 870
BstH2I RGCGCY 1 cut(s) 482
BstHHI GCGC 1 cut(s) 481
BstKTI GATC 6 cut(s) 61, 195, 244, 261, 279, 348
BstMAI GTCTC 1 cut(s) 479
BstMBI GATC 6 cut(s) 58, 192, 241, 258, 276, 345
BstMWI GCNNNNNNNGC 6 cut(s) 98, 294, 358, 402, 732, 824
BstNI CCWGG 5 cut(s) 315, 575, 681, 701, 783
BstNSI RCATGY 2 cut(s) 848, 930
BstPAI GACNNNNGTC 2 cut(s) 8, 535
BstSFI CTRYAG 1 cut(s) 819
BstUI CGCG 1 cut(s) 870
BstV1I GCAGC 1 cut(s) 916
BstV2I GAAGAC 2 cut(s) 333, 1166
BstXI CCANNNNNNTGG 1 cut(s) 356
BstZ17I GTATAC 1 cut(s) 943
BsuI GTATCC 2 cut(s) 930, 1104
BsuRI GGCC 6 cut(s) 282, 313, 435, 445, 768, 1040
BtgZI GCGATG 1 cut(s) 274
BtsCI GGATG 4 cut(s) 244, 711, 760, 956
BtsIMutI CAGTG 1 cut(s) 546
Cac8I GCNNGC 6 cut(s) 94, 295, 403, 706, 846, 928
CciI TCATGA 1 cut(s) 889
CfoI GCGC 1 cut(s) 481
Cfr10I RCCGGY 2 cut(s) 92, 293
Cfr13I GGNCC 3 cut(s) 427, 433, 767
Cfr9I CCCGGG 1 cut(s) 464
CseI GACGC 2 cut(s) 286, 876
Csp6I GTAC 2 cut(s) 124, 148
CviAII CATG 5 cut(s) 845, 890, 901, 927, 1042
CviQI GTAC 2 cut(s) 124, 148
DdeI CTNAG 1 cut(s) 966
DpnI GATC 6 cut(s) 60, 194, 243, 260, 278, 347
DpnII GATC 6 cut(s) 58, 192, 241, 258, 276, 345
DriI GACNNNNNGTC 1 cut(s) 492
EaeI YGGCCR 2 cut(s) 311, 1038
Eam1104I CTCTTC 1 cut(s) 108
Eam1105I GACNNNNNGTC 1 cut(s) 492
EarI CTCTTC 1 cut(s) 108
Eco47I GGWCC 1 cut(s) 427
Eco47III AGCGCT 1 cut(s) 480
Eco57I CTGAAG 1 cut(s) 626
Eco88I CYCGRG 1 cut(s) 464
EcoRI GAATTC 2 cut(s) 458, 589
EcoRII CCWGG 5 cut(s) 313, 573, 679, 699, 781
EcoT22I ATGCAT 1 cut(s) 290
FaeI CATG 5 cut(s) 848, 893, 904, 930, 1045
FaqI GGGAC 2 cut(s) 183, 661
FatI CATG 5 cut(s) 844, 889, 900, 926, 1041
FauI CCCGC 1 cut(s) 360
FauNDI CATATG 1 cut(s) 498
FbaI TGATCA 1 cut(s) 241
FblI GTMKAC 2 cut(s) 156, 942
Fnu4HI GCNGC 5 cut(s) 362, 365, 406, 905, 1038
FokI GGATG 4 cut(s) 251, 698, 747, 943
Fsp4HI GCNGC 5 cut(s) 362, 365, 406, 905, 1038
FspBI CTAG 2 cut(s) 620, 1032
GlaI GCGC 1 cut(s) 480
GluI GCNGC 5 cut(s) 362, 365, 406, 905, 1038
GsaI CCCAGC 1 cut(s) 337
HaeII RGCGCY 1 cut(s) 482
HaeIII GGCC 6 cut(s) 282, 313, 435, 445, 768, 1040
HgaI GACGC 2 cut(s) 286, 876
HhaI GCGC 1 cut(s) 481
Hin1I GRCGYC 2 cut(s) 172, 297
Hin1II CATG 5 cut(s) 848, 893, 904, 930, 1045
Hin6I GCGC 1 cut(s) 479
HinP1I GCGC 1 cut(s) 479
HincII GTYRAC 1 cut(s) 157
HindII GTYRAC 1 cut(s) 157
HindIII AAGCTT 1 cut(s) 522
HinfI GANTC 3 cut(s) 684, 947, 1130
HphI GGTGA 1 cut(s) 64
Hpy166II GTNNAC 4 cut(s) 124, 157, 549, 943
Hpy188I TCNGA 4 cut(s) 606, 969, 1135, 1190
Hpy188III TCNNGA 6 cut(s) 190, 262, 415, 839, 890, 1105
Hpy8I GTNNAC 4 cut(s) 124, 157, 549, 943
Hpy99I CGWCG 4 cut(s) 161, 177, 302, 833
HpyAV CCTTC 6 cut(s) 93, 267, 502, 655, 780, 1082
HpyCH4III ACNGT 5 cut(s) 152, 165, 494, 1140, 1181
HpyCH4IV ACGT 2 cut(s) 5, 172
HpyCH4V TGCA 9 cut(s) 223, 288, 396, 544, 883, 904, 930, 1002, 1068
HpyF10VI GCNNNNNNNGC 6 cut(s) 98, 294, 358, 402, 732, 824
HpyF3I CTNAG 1 cut(s) 966
HpySE526I ACGT 2 cut(s) 5, 172
Hsp92I GRCGYC 2 cut(s) 172, 297
Hsp92II CATG 5 cut(s) 848, 893, 904, 930, 1045
HspAI GCGC 1 cut(s) 479
Kpn2I TCCGGA 1 cut(s) 838
KpnI GGTACC 1 cut(s) 151
KroI GCCGGC 2 cut(s) 92, 293
KroNI GCCGGC 2 cut(s) 94, 295
Ksp22I TGATCA 1 cut(s) 241
Kzo9I GATC 6 cut(s) 58, 192, 241, 258, 276, 345
LmnI GCTCC 2 cut(s) 824, 841
Lsp1109I GCAGC 1 cut(s) 916
LweI GCATC 3 cut(s) 297, 717, 965
MaeI CTAG 2 cut(s) 620, 1032
MaeII ACGT 2 cut(s) 5, 172
MaeIII GTNAC 1 cut(s) 13
MalI GATC 6 cut(s) 60, 194, 243, 260, 278, 347
MbiI CCGCTC 1 cut(s) 1037
MboI GATC 6 cut(s) 58, 192, 241, 258, 276, 345
MboII GAAGA 5 cut(s) 125, 173, 333, 585, 1171
MfeI CAATTG 1 cut(s) 729
MluCI AATT 5 cut(s) 458, 589, 633, 729, 775
MluI ACGCGT 1 cut(s) 868
MmeI TCCRAC 3 cut(s) 112, 844, 1121
Mph1103I ATGCAT 1 cut(s) 290
MreI CGCCGGCG 1 cut(s) 293
MroI TCCGGA 1 cut(s) 838
MroNI GCCGGC 2 cut(s) 92, 293
MroXI GAANNNNTTC 1 cut(s) 990
MseI TTAA 2 cut(s) 632, 1116
MslI CAYNNNNRTG 2 cut(s) 266, 935
MspA1I CMGCKG 3 cut(s) 106, 517, 818
MunI CAATTG 1 cut(s) 729
MvaI CCWGG 5 cut(s) 315, 575, 681, 701, 783
MvnI CGCG 1 cut(s) 870
MwoI GCNNNNNNNGC 6 cut(s) 98, 294, 358, 402, 732, 824
NaeI GCCGGC 2 cut(s) 94, 295
NciI CCSGG 5 cut(s) 425, 431, 465, 466, 765
NdeI CATATG 1 cut(s) 498
NdeII GATC 6 cut(s) 58, 192, 241, 258, 276, 345
NgoMIV GCCGGC 2 cut(s) 92, 293
NlaIII CATG 5 cut(s) 848, 893, 904, 930, 1045
NlaIV GGNNCC 8 cut(s) 149, 422, 428, 434, 787, 826, 852, 1059
NsiI ATGCAT 1 cut(s) 290
NspI RCATGY 2 cut(s) 848, 930
PaeI GCATGC 2 cut(s) 848, 930
PagI TCATGA 1 cut(s) 889
PcsI WCGNNNNNNNCGW 1 cut(s) 297
PdiI GCCGGC 2 cut(s) 94, 295
PdmI GAANNNNTTC 1 cut(s) 990
PfeI GAWTC 3 cut(s) 684, 947, 1130
PfoI TCCNGGA 1 cut(s) 781
PkrI GCNGC 5 cut(s) 363, 366, 407, 906, 1039
PshAI GACNNNNGTC 2 cut(s) 8, 535
PshBI ATTAAT 1 cut(s) 632
Psp6I CCWGG 5 cut(s) 313, 573, 679, 699, 781
PspFI CCCAGC 1 cut(s) 333
PspGI CCWGG 5 cut(s) 313, 573, 679, 699, 781
PspN4I GGNNCC 8 cut(s) 149, 422, 428, 434, 787, 826, 852, 1059
PspPI GGNCC 3 cut(s) 427, 433, 767
PvuII CAGCTG 3 cut(s) 106, 517, 818
RsaI GTAC 2 cut(s) 125, 149
RsaNI GTAC 2 cut(s) 124, 148
RseI CAYNNNNRTG 2 cut(s) 266, 935
SalI GTCGAC 1 cut(s) 155
SaqAI TTAA 2 cut(s) 632, 1116
SatI GCNGC 5 cut(s) 362, 365, 406, 905, 1038
Sau3AI GATC 6 cut(s) 58, 192, 241, 258, 276, 345
Sau96I GGNCC 3 cut(s) 427, 433, 767
SfaNI GCATC 3 cut(s) 297, 717, 965
SfcI CTRYAG 1 cut(s) 819
SgrAI CRCCGGYG 1 cut(s) 293
SinI GGWCC 1 cut(s) 427
SmaI CCCGGG 1 cut(s) 466
SmiMI CAYNNNNRTG 2 cut(s) 266, 935
SmlI CTYRAG 1 cut(s) 482
SmoI CTYRAG 1 cut(s) 482
SphI GCATGC 2 cut(s) 848, 930
Sse9I AATT 5 cut(s) 458, 589, 633, 729, 775
SsiI CCGC 7 cut(s) 361, 364, 367, 405, 613, 848, 1037
SspMI CTAG 2 cut(s) 620, 1032
TaaI ACNGT 5 cut(s) 152, 165, 494, 1140, 1181
TaiI ACGT 2 cut(s) 8, 175
TaqI TCGA 5 cut(s) 156, 175, 300, 344, 563
TaqII GACCGA 1 cut(s) 1100
TasI AATT 5 cut(s) 458, 589, 633, 729, 775
TauI GCSGC 4 cut(s) 364, 367, 408, 1040
TfiI GAWTC 3 cut(s) 684, 947, 1130
Tru1I TTAA 2 cut(s) 632, 1116
Tru9I TTAA 2 cut(s) 632, 1116
TscAI CASTG 1 cut(s) 546
TseI GCWGC 1 cut(s) 904
TspDTI ATGAA 2 cut(s) 878, 939
TspGWI ACGGA 2 cut(s) 76, 1132
TspMI CCCGGG 1 cut(s) 464
TspRI CASTG 1 cut(s) 546
VpaK11BI GGWCC 1 cut(s) 427
VspI ATTAAT 1 cut(s) 632
XapI RAATTY 2 cut(s) 458, 589
XceI RCATGY 2 cut(s) 848, 930
XmaI CCCGGG 1 cut(s) 464
XmiI GTMKAC 2 cut(s) 156, 942
XmnI GAANNNNTTC 1 cut(s) 990
XspI CTAG 2 cut(s) 620, 1032
ZraI GACGTC 1 cut(s) 173
Zsp2I ATGCAT 1 cut(s) 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.