Rroxscaffold_4G00309340

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0003970
Physical Location & Seq
Reverse (-)
31093428 .. 31095341
1914 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00309340.1

Sequence Viewer

Length: 582 bp
ATGGTCACCCAATCGCCTGCTGCGTGGGCCCCATCATTTGGTCGACTAATTTTGAACCCGTTCATTAATCCACCGGCGCTTTCTCGGCAATTGATCAACCAATTACCCTTCCGGTGCATCTCCTCTTACGACAATTCCACCCCAATTCCGGATGAATCACTTGTTGACCAATGGTTTGCAGCAAAAAGCGTGGATCACTCTTGCCGGATTACCGGCAAGATTTGGCCTTGTTACTCGATACCTCTTGGGGACGAATATATTGTTGTGGGAGGTTGGCCAAAATTCTTGCGGCATATTAGTGCAGGCCAATCCCACCTCCGACTTATTTTCCAATTCCATTCCACTACAATGACGTTCGTTGTTACCAGGTTTGATTCTTTCGGTTATCTACCGCGCTCCCCACCCGATCTACTCCACTGCGGTGTTCAACCCTCCATAGCTGGCTTGGATCAGCTTAAAGAAGAGTATGTACACAATATTGGAACACACAAGTGGTACCCGGTTGATGAACGGTTGGTAAGCATTGCAGTCCTTCTATCACGACGTACCCAAAACTGTTCTTCACGGTGTATGAGTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

193

Amino Acids

21.82

Weight (kDa)

8.58

Isoelectric Point (pI)

56.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 495
AccB1I GGYRCC 1 cut(s) 495
AccB7I CCANNNNNTGG 1 cut(s) 38
AccI GTMKAC 1 cut(s) 43
AccII CGCG 1 cut(s) 394
AccIII TCCGGA 1 cut(s) 148
AciI CCGC 3 cut(s) 289, 392, 420
AclWI GGATC 2 cut(s) 201, 456
AcoI YGGCCR 1 cut(s) 275
AcsI RAATTY 1 cut(s) 281
AfaI GTAC 3 cut(s) 471, 497, 547
AfiI CCNNNNNNNGG 2 cut(s) 38, 148
AgsI TTSAA 2 cut(s) 55, 428
AjnI CCWGG 1 cut(s) 365
AleI CACNNNNGTG 2 cut(s) 420, 490
AluBI AGCT 2 cut(s) 440, 454
AluI AGCT 2 cut(s) 440, 454
AlwI GGATC 2 cut(s) 201, 456
Aor13HI TCCGGA 1 cut(s) 148
AoxI GGCC 4 cut(s) 27, 224, 275, 304
ApaI GGGCCC 1 cut(s) 31
ApeKI GCWGC 2 cut(s) 20, 179
ApoI RAATTY 1 cut(s) 281
ArsI GACNNNNNNTTYG 2 cut(s) 158, 190
AseI ATTAAT 1 cut(s) 66
Asp700I GAANNNNTTC 1 cut(s) 59
Asp718I GGTACC 1 cut(s) 495
AspLEI GCGC 2 cut(s) 79, 396
AspS9I GGNCC 2 cut(s) 27, 28
AsuC2I CCSGG 1 cut(s) 500
BaeGI GKGCMC 1 cut(s) 31
BalI TGGCCA 1 cut(s) 277
BanI GGYRCC 1 cut(s) 495
BanII GRGCYC 1 cut(s) 31
BarI GAAGNNNNNNTAC 2 cut(s) 453, 485
BbvI GCAGC 2 cut(s) 7, 191
BccI CCATC 1 cut(s) 40
BciT130I CCWGG 1 cut(s) 367
BclI TGATCA 1 cut(s) 93
BcnI CCSGG 1 cut(s) 500
BfoI RGCGCY 1 cut(s) 80
BisI GCNGC 3 cut(s) 21, 180, 290
BlsI GCNGC 3 cut(s) 22, 181, 291
Bme1390I CCNGG 2 cut(s) 367, 500
BmgT120I GGNCC 2 cut(s) 27, 28
BmiI GGNNCC 3 cut(s) 29, 30, 497
BmrFI CCNGG 2 cut(s) 367, 500
BmsI GCATC 1 cut(s) 126
BpuMI CCSGG 1 cut(s) 500
BsaWI WCCGGW 2 cut(s) 111, 148
Bsc4I CCNNNNNNNGG 2 cut(s) 38, 148
Bse118I RCCGGY 2 cut(s) 73, 212
Bse3DI GCAATG 1 cut(s) 522
BseAI TCCGGA 1 cut(s) 148
BseBI CCWGG 1 cut(s) 367
BseGI GGATG 1 cut(s) 157
BseLI CCNNNNNNNGG 2 cut(s) 38, 148
BseMI GCAATG 1 cut(s) 522
BseRI GAGGAG 1 cut(s) 112
BseSI GKGCMC 1 cut(s) 31
BseXI GCAGC 2 cut(s) 7, 191
BsgI GTGCAG 1 cut(s) 321
Bsh1236I CGCG 1 cut(s) 394
BshFI GGCC 4 cut(s) 29, 226, 277, 306
BshNI GGYRCC 1 cut(s) 495
BsiSI CCGG 6 cut(s) 74, 112, 149, 205, 213, 500
BslFI GGGAC 1 cut(s) 263
BslI CCNNNNNNNGG 2 cut(s) 38, 148
BsmFI GGGAC 1 cut(s) 263
BsnI GGCC 4 cut(s) 29, 226, 277, 306
Bsp120I GGGCCC 1 cut(s) 27
Bsp1286I GDGCHC 1 cut(s) 31
Bsp13I TCCGGA 1 cut(s) 148
Bsp1407I TGTACA 1 cut(s) 469
Bsp143I GATC 4 cut(s) 93, 193, 406, 448
BspACI CCGC 3 cut(s) 289, 392, 420
BspANI GGCC 4 cut(s) 29, 226, 277, 306
BspEI TCCGGA 1 cut(s) 148
BspFNI CGCG 1 cut(s) 394
BspLI GGNNCC 3 cut(s) 29, 30, 497
BspPI GGATC 2 cut(s) 201, 456
BspT107I GGYRCC 1 cut(s) 495
BsrDI GCAATG 1 cut(s) 522
BsrFI RCCGGY 2 cut(s) 73, 212
BsrGI TGTACA 1 cut(s) 469
BssAI RCCGGY 2 cut(s) 73, 212
BssMI GATC 4 cut(s) 93, 193, 406, 448
Bst2UI CCWGG 1 cut(s) 367
Bst4CI ACNGT 3 cut(s) 513, 557, 567
Bst6I CTCTTC 1 cut(s) 456
BstAUI TGTACA 1 cut(s) 469
BstC8I GCNNGC 3 cut(s) 18, 304, 442
BstEII GGTNACC 1 cut(s) 4
BstF5I GGATG 1 cut(s) 157
BstFNI CGCG 1 cut(s) 394
BstH2I RGCGCY 1 cut(s) 80
BstHHI GCGC 2 cut(s) 79, 396
BstKTI GATC 4 cut(s) 96, 196, 409, 451
BstMBI GATC 4 cut(s) 93, 193, 406, 448
BstMWI GCNNNNNNNGC 2 cut(s) 26, 85
BstNI CCWGG 1 cut(s) 367
BstPI GGTNACC 1 cut(s) 4
BstSCI CCNGG 2 cut(s) 365, 498
BstSLI GKGCMC 1 cut(s) 31
BstUI CGCG 1 cut(s) 394
BstV1I GCAGC 2 cut(s) 7, 191
BsuRI GGCC 4 cut(s) 29, 226, 277, 306
BtsCI GGATG 1 cut(s) 157
BtsI GCAGTG 1 cut(s) 415
BtsIMutI CAGTG 1 cut(s) 415
Cac8I GCNNGC 3 cut(s) 18, 304, 442
CfoI GCGC 2 cut(s) 79, 396
Cfr10I RCCGGY 2 cut(s) 73, 212
Cfr13I GGNCC 2 cut(s) 27, 28
CsiI ACCWGGT 1 cut(s) 365
Csp6I GTAC 3 cut(s) 470, 496, 546
CspCI CAANNNNNGTGG 2 cut(s) 171, 206
CviJI RGCY 7 cut(s) 29, 226, 277, 306, 440, 444, 454
CviKI_1 RGCY 7 cut(s) 29, 226, 277, 306, 440, 444, 454
CviQI GTAC 3 cut(s) 470, 496, 546
DpnI GATC 4 cut(s) 95, 195, 408, 450
DpnII GATC 4 cut(s) 93, 193, 406, 448
EaeI YGGCCR 1 cut(s) 275
Eam1104I CTCTTC 1 cut(s) 456
EarI CTCTTC 1 cut(s) 456
Eco24I GRGCYC 1 cut(s) 31
Eco91I GGTNACC 1 cut(s) 4
EcoO109I RGGNCCY 1 cut(s) 28
EcoO65I GGTNACC 1 cut(s) 4
EcoRII CCWGG 1 cut(s) 365
EcoT38I GRGCYC 1 cut(s) 31
FaiI YATR 5 cut(s) 258, 294, 437, 468, 572
FaqI GGGAC 1 cut(s) 263
FbaI TGATCA 1 cut(s) 93
FblI GTMKAC 1 cut(s) 43
Fnu4HI GCNGC 3 cut(s) 21, 180, 290
FokI GGATG 1 cut(s) 164
FriOI GRGCYC 1 cut(s) 31
Fsp4HI GCNGC 3 cut(s) 21, 180, 290
GlaI GCGC 2 cut(s) 78, 395
GluI GCNGC 3 cut(s) 21, 180, 290
HaeII RGCGCY 1 cut(s) 80
HaeIII GGCC 4 cut(s) 29, 226, 277, 306
HapII CCGG 6 cut(s) 74, 112, 149, 205, 213, 500
HhaI GCGC 2 cut(s) 79, 396
Hin6I GCGC 2 cut(s) 77, 394
HinP1I GCGC 2 cut(s) 77, 394
HincII GTYRAC 2 cut(s) 44, 166
HindII GTYRAC 2 cut(s) 44, 166
HinfI GANTC 2 cut(s) 155, 374
HpaII CCGG 6 cut(s) 74, 112, 149, 205, 213, 500
Hpy166II GTNNAC 3 cut(s) 44, 166, 472
Hpy188I TCNGA 1 cut(s) 320
Hpy188III TCNNGA 2 cut(s) 149, 540
Hpy8I GTNNAC 3 cut(s) 44, 166, 472
Hpy99I CGWCG 1 cut(s) 546
HpyAV CCTTC 2 cut(s) 118, 542
HpyCH4III ACNGT 3 cut(s) 513, 557, 567
HpyCH4IV ACGT 2 cut(s) 353, 544
HpyCH4V TGCA 4 cut(s) 117, 179, 302, 527
HpyF10VI GCNNNNNNNGC 2 cut(s) 26, 85
HpySE526I ACGT 2 cut(s) 353, 544
HspAI GCGC 2 cut(s) 77, 394
Kpn2I TCCGGA 1 cut(s) 148
KpnI GGTACC 1 cut(s) 499
Ksp22I TGATCA 1 cut(s) 93
Kzo9I GATC 4 cut(s) 93, 193, 406, 448
LmnI GCTCC 1 cut(s) 401
Lsp1109I GCAGC 2 cut(s) 7, 191
LweI GCATC 1 cut(s) 126
MabI ACCWGGT 1 cut(s) 365
MaeII ACGT 2 cut(s) 353, 544
MaeIII GTNAC 3 cut(s) 4, 230, 361
MalI GATC 4 cut(s) 95, 195, 408, 450
MboI GATC 4 cut(s) 93, 193, 406, 448
MboII GAAGA 2 cut(s) 473, 552
MfeI CAATTG 1 cut(s) 89
MhlI GDGCHC 1 cut(s) 31
MlsI TGGCCA 1 cut(s) 277
MluCI AATT 7 cut(s) 48, 89, 101, 133, 144, 281, 332
MluNI TGGCCA 1 cut(s) 277
MmeI TCCRAC 1 cut(s) 343
MnlI CCTC 5 cut(s) 133, 252, 263, 326, 442
Mox20I TGGCCA 1 cut(s) 277
MroI TCCGGA 1 cut(s) 148
MroXI GAANNNNTTC 1 cut(s) 59
MscI TGGCCA 1 cut(s) 277
MseI TTAA 2 cut(s) 66, 456
MslI CAYNNNNRTG 4 cut(s) 297, 347, 420, 490
Msp20I TGGCCA 1 cut(s) 277
MspI CCGG 6 cut(s) 74, 112, 149, 205, 213, 500
MspR9I CCNGG 2 cut(s) 367, 500
MunI CAATTG 1 cut(s) 89
MvaI CCWGG 1 cut(s) 367
MvnI CGCG 1 cut(s) 394
MwoI GCNNNNNNNGC 2 cut(s) 26, 85
NciI CCSGG 1 cut(s) 500
NdeII GATC 4 cut(s) 93, 193, 406, 448
NlaIV GGNNCC 3 cut(s) 29, 30, 497
NmeAIII GCCGAG 1 cut(s) 64
NmuCI GTSAC 1 cut(s) 4
OliI CACNNNNGTG 2 cut(s) 420, 490
PcsI WCGNNNNNNNCGW 1 cut(s) 20
PdmI GAANNNNTTC 1 cut(s) 59
PfeI GAWTC 2 cut(s) 155, 374
PflMI CCANNNNNTGG 1 cut(s) 38
PkrI GCNGC 3 cut(s) 22, 181, 291
PshBI ATTAAT 1 cut(s) 66
Psp6I CCWGG 1 cut(s) 365
PspEI GGTNACC 1 cut(s) 4
PspGI CCWGG 1 cut(s) 365
PspN4I GGNNCC 3 cut(s) 29, 30, 497
PspOMI GGGCCC 1 cut(s) 27
PspPI GGNCC 2 cut(s) 27, 28
RsaI GTAC 3 cut(s) 471, 497, 547
RsaNI GTAC 3 cut(s) 470, 496, 546
RseI CAYNNNNRTG 4 cut(s) 297, 347, 420, 490
SalI GTCGAC 1 cut(s) 42
SaqAI TTAA 2 cut(s) 66, 456
SatI GCNGC 3 cut(s) 21, 180, 290
Sau3AI GATC 4 cut(s) 93, 193, 406, 448
Sau96I GGNCC 2 cut(s) 27, 28
ScrFI CCNGG 2 cut(s) 367, 500
SduI GDGCHC 1 cut(s) 31
SetI ASST 8 cut(s) 244, 274, 318, 356, 371, 442, 456, 547
SexAI ACCWGGT 1 cut(s) 365
SfaNI GCATC 1 cut(s) 126
SgrAI CRCCGGYG 1 cut(s) 73
SmiMI CAYNNNNRTG 4 cut(s) 297, 347, 420, 490
Sse9I AATT 7 cut(s) 48, 89, 101, 133, 144, 281, 332
SsiI CCGC 3 cut(s) 289, 392, 420
SspI AATATT 1 cut(s) 478
StyD4I CCNGG 2 cut(s) 365, 498
TaaI ACNGT 3 cut(s) 513, 557, 567
TaiI ACGT 2 cut(s) 356, 547
TaqI TCGA 2 cut(s) 43, 236
TasI AATT 7 cut(s) 48, 89, 101, 133, 144, 281, 332
TatI WGTACW 1 cut(s) 469
TauI GCSGC 1 cut(s) 292
TfiI GAWTC 2 cut(s) 155, 374
Tru1I TTAA 2 cut(s) 66, 456
Tru9I TTAA 2 cut(s) 66, 456
TscAI CASTG 1 cut(s) 422
TseFI GTSAC 1 cut(s) 4
TseI GCWGC 2 cut(s) 20, 179
Tsp45I GTSAC 1 cut(s) 4
TspDTI ATGAA 3 cut(s) 52, 168, 522
TspRI CASTG 1 cut(s) 422
Van91I CCANNNNNTGG 1 cut(s) 38
VspI ATTAAT 1 cut(s) 66
XapI RAATTY 1 cut(s) 281
XcmI CCANNNNNNNNNTGG 1 cut(s) 442
XmiI GTMKAC 1 cut(s) 43
XmnI GAANNNNTTC 1 cut(s) 59
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.