Rroxscaffold_7G00162700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0003970
Physical Location & Seq
Forward (+)
5071185 .. 5073691
2507 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00162700.1

Sequence Viewer

Length: 1305 bp
ATGGGAGAGCTCGTCCAAATAGTCTTGGTGGAGCCGGGGACCGACCCTTTCGTGGCAACGATTTCTTGCAATATGGTGACTGCTCTCGACTTGCGGACGGAAAATACACCGACAGTAACAATTGTGATTAGACGGGACAGAGACACGAGAGGAGAGAGAGCTAATTATGGCCAAGGTATTATTGTGTATCATCATACTATGGGCTACCCATTCAAAAAGCGTGGATCACTCTTGCTTTGGATTACCGGCAAGATTTGGCCTTGTTACTCGATACCTCTTGGGGACGAATATATTGTTGTGGGAGGTTGGCCAAAATTCTTGCGGCATATTAGTGCAGGCCAATCCCACCTCCGACTTATTTTCCAATTCCATTCCACTACAATGACGTTCGTTGTTACCAGGTTTGATTCTTTCGGTTATCTACTGCGCTCCCCACCCGATCTACTCCACTGCGGTGTTCAACCCTCCATAGCTGGCTTGGATCAGCTTAAAGAAGAGTATGTACACAATATTGGAACACACAAGTGGTACCCGGTTGATGAACGGTTGCAGAATTCCATCTTCATACAACCTTCACTTGATACCCATCGTCATATATGTTGGACAATACAAGTGGAGAATTATGGTCTTCAAGCATTGCGGTTTGATGACGGGTGGCCCAATTTTGCACACCAAACCGGGTTAAATTCTTCCCACCGTATCAAGTTCAGGCTGCTGGAAGGTCGGCTCATTACGGCCGTCATTTTCCATCGTCGAAAGGGCCATTTGGTGACCCCCGTATTGAGTAATCCCAACCCCGCCAGCGGCGGGGCATGTGTTGGTTCTTATCAATCTACTTCGATAGCCGGTCCTTCGAATGCGGGACCCTCCATTTCCCATGCACCTTCCTCCCCAATAGGCACCACCTCGGCTGAAGTAGGTGCGGTTGATGAACCCGACTGTCCCGAAACATATGTAATTGGACGCGTTTGGCAAATGCAGGTGCATGACCTTATATTCCAGCAACTCCCATCCTTTCCCACGTGTACCATGATTGTATACGATACTTTCGCTGTAAGAAATCTCTCTCACATGTACTTGCCTCAAAGTTTTGTCCATTCCCGAACATTGCACCGCGCAAAGATGTCTTCTGTTCCGAGTTTTTCGGGAGGTGCAATGCTATTGAAGCCGACTCCGCACTGCGAAACCCTATTTGTCGGCGAAGGGTACCCGACCTTCAAGCAGCATTTTCGGTTGACAACGAACTCCCCACTTTCACTCCATCCGGTTGGAAACACTCATTTTGTTTTCAACGGCCCTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

434

Amino Acids

48.49

Weight (kDa)

8.57

Isoelectric Point (pI)

41.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 970
Acc36I ACCTGC 1 cut(s) 970
Acc65I GGTACC 2 cut(s) 528, 1206
AccB1I GGYRCC 3 cut(s) 528, 899, 1206
AccI GTMKAC 1 cut(s) 1038
AccII CGCG 2 cut(s) 966, 1116
AclWI GGATC 2 cut(s) 232, 489
AcoI YGGCCR 3 cut(s) 169, 308, 735
AcsI RAATTY 3 cut(s) 314, 553, 685
AcuI CTGAAG 1 cut(s) 933
AcvI CACGTG 1 cut(s) 1023
AfaI GTAC 5 cut(s) 504, 530, 1027, 1076, 1208
AfiI CCNNNNNNNGG 3 cut(s) 52, 803, 807
AflIII ACRYGT 3 cut(s) 964, 1022, 1071
AgsI TTSAA 6 cut(s) 214, 461, 632, 1165, 1219, 1291
AjnI CCWGG 1 cut(s) 398
AleI CACNNNNGTG 2 cut(s) 453, 523
AluBI AGCT 4 cut(s) 10, 161, 473, 487
AluI AGCT 4 cut(s) 10, 161, 473, 487
Alw21I GWGCWC 1 cut(s) 12
Alw26I GTCTC 1 cut(s) 135
AlwI GGATC 2 cut(s) 232, 489
AoxI GGCC 8 cut(s) 169, 257, 308, 337, 656, 735, 760, 1294
ApeKI GCWGC 2 cut(s) 712, 1222
ApoI RAATTY 3 cut(s) 314, 553, 685
Asp718I GGTACC 2 cut(s) 528, 1206
AspLEI GCGC 2 cut(s) 429, 1118
AspS9I GGNCC 6 cut(s) 39, 657, 760, 848, 863, 1295
AsuC2I CCSGG 3 cut(s) 36, 533, 679
AsuHPI GGTGA 2 cut(s) 88, 781
AsuII TTCGAA 1 cut(s) 854
AvaII GGWCC 3 cut(s) 39, 848, 863
BalI TGGCCA 2 cut(s) 171, 310
BanI GGYRCC 3 cut(s) 528, 899, 1206
BanII GRGCYC 1 cut(s) 12
BarI GAAGNNNNNNTAC 4 cut(s) 486, 518, 1199, 1231
BauI CACGAG 1 cut(s) 145
BbrPI CACGTG 1 cut(s) 1023
BbsI GAAGAC 2 cut(s) 620, 1119
Bbv12I GWGCWC 1 cut(s) 12
BbvI GCAGC 2 cut(s) 699, 1234
BccI CCATC 5 cut(s) 566, 594, 756, 1018, 1269
BceAI ACGGC 2 cut(s) 722, 750
BcgI CGANNNNNNTGC 2 cut(s) 1211, 1245
BciT130I CCWGG 1 cut(s) 400
BcnI CCSGG 3 cut(s) 36, 533, 679
BcoDI GTCTC 1 cut(s) 135
BfuAI ACCTGC 1 cut(s) 970
BisI GCNGC 4 cut(s) 323, 713, 805, 1223
BlsI GCNGC 4 cut(s) 324, 714, 806, 1224
Bme1390I CCNGG 4 cut(s) 36, 400, 533, 679
Bme18I GGWCC 3 cut(s) 39, 848, 863
BmgT120I GGNCC 6 cut(s) 39, 657, 760, 848, 863, 1295
BmiI GGNNCC 7 cut(s) 33, 40, 530, 864, 865, 901, 1208
BmrFI CCNGG 4 cut(s) 36, 400, 533, 679
BpiI GAAGAC 2 cut(s) 620, 1119
Bpu14I TTCGAA 1 cut(s) 854
BpuMI CCSGG 3 cut(s) 36, 533, 679
BsaAI YACGTR 1 cut(s) 1023
BsaBI GATNNNNATC 1 cut(s) 585
BsaJI CCNNGG 3 cut(s) 35, 172, 906
BsaWI WCCGGW 1 cut(s) 1264
BsaXI ACNNNNNCTCC 3 cut(s) 27, 1242, 1272
Bsc4I CCNNNNNNNGG 3 cut(s) 52, 803, 807
Bse118I RCCGGY 2 cut(s) 245, 845
Bse3DI GCAATG 3 cut(s) 635, 1106, 1161
Bse8I GATNNNNATC 1 cut(s) 585
BseBI CCWGG 1 cut(s) 400
BseDI CCNNGG 3 cut(s) 35, 172, 906
BseGI GGATG 2 cut(s) 1010, 1261
BseJI GATNNNNATC 1 cut(s) 585
BseLI CCNNNNNNNGG 3 cut(s) 52, 803, 807
BseMI GCAATG 3 cut(s) 635, 1106, 1161
BseRI GAGGAG 1 cut(s) 165
BseX3I CGGCCG 1 cut(s) 735
BseXI GCAGC 2 cut(s) 699, 1234
BsgI GTGCAG 1 cut(s) 354
Bsh1236I CGCG 2 cut(s) 966, 1116
Bsh1285I CGRYCG 1 cut(s) 738
BshFI GGCC 8 cut(s) 171, 259, 310, 339, 658, 737, 762, 1296
BshNI GGYRCC 3 cut(s) 528, 899, 1206
BsiEI CGRYCG 1 cut(s) 738
BsiHKAI GWGCWC 1 cut(s) 12
BsiSI CCGG 6 cut(s) 35, 246, 533, 678, 846, 1265
BslFI GGGAC 5 cut(s) 52, 149, 296, 876, 927
BslI CCNNNNNNNGG 3 cut(s) 52, 803, 807
BsmAI GTCTC 1 cut(s) 135
BsmFI GGGAC 5 cut(s) 52, 149, 296, 876, 927
BsmI GAATGC 1 cut(s) 862
BsnI GGCC 8 cut(s) 171, 259, 310, 339, 658, 737, 762, 1296
Bsp119I TTCGAA 1 cut(s) 854
Bsp1286I GDGCHC 1 cut(s) 12
Bsp1407I TGTACA 1 cut(s) 502
Bsp143I GATC 3 cut(s) 224, 439, 481
BspANI GGCC 8 cut(s) 171, 259, 310, 339, 658, 737, 762, 1296
BspFNI CGCG 2 cut(s) 966, 1116
BspLI GGNNCC 7 cut(s) 33, 40, 530, 864, 865, 901, 1208
BspMI ACCTGC 1 cut(s) 970
BspPI GGATC 2 cut(s) 232, 489
BspT104I TTCGAA 1 cut(s) 854
BspT107I GGYRCC 3 cut(s) 528, 899, 1206
BsrDI GCAATG 3 cut(s) 635, 1106, 1161
BsrFI RCCGGY 2 cut(s) 245, 845
BsrGI TGTACA 1 cut(s) 502
BssAI RCCGGY 2 cut(s) 245, 845
BssECI CCNNGG 3 cut(s) 35, 172, 906
BssMI GATC 3 cut(s) 224, 439, 481
BssNAI GTATAC 1 cut(s) 1039
BssSI CACGAG 1 cut(s) 145
BssT1I CCWWGG 1 cut(s) 172
Bst1107I GTATAC 1 cut(s) 1039
Bst2BI CACGAG 1 cut(s) 145
Bst2UI CCWGG 1 cut(s) 400
Bst4CI ACNGT 4 cut(s) 115, 546, 698, 941
Bst6I CTCTTC 1 cut(s) 489
BstAUI TGTACA 1 cut(s) 502
BstBAI YACGTR 1 cut(s) 1023
BstBI TTCGAA 1 cut(s) 854
BstC8I GCNNGC 3 cut(s) 337, 475, 802
BstEII GGTNACC 1 cut(s) 769
BstF5I GGATG 2 cut(s) 1010, 1261
BstFNI CGCG 2 cut(s) 966, 1116
BstHHI GCGC 2 cut(s) 429, 1118
BstKTI GATC 3 cut(s) 227, 442, 484
BstMAI GTCTC 1 cut(s) 135
BstMBI GATC 3 cut(s) 224, 439, 481
BstMCI CGRYCG 1 cut(s) 738
BstMWI GCNNNNNNNGC 2 cut(s) 1165, 1174
BstNI CCWGG 1 cut(s) 400
BstNSI RCATGY 2 cut(s) 816, 1075
BstPI GGTNACC 1 cut(s) 769
BstSCI CCNGG 4 cut(s) 34, 398, 531, 677
BstUI CGCG 2 cut(s) 966, 1116
BstV1I GCAGC 2 cut(s) 699, 1234
BstV2I GAAGAC 2 cut(s) 620, 1119
BstXI CCANNNNNNTGG 1 cut(s) 1268
BstZ17I GTATAC 1 cut(s) 1039
BstZI CGGCCG 1 cut(s) 735
BsuRI GGCC 8 cut(s) 171, 259, 310, 339, 658, 737, 762, 1296
BtsCI GGATG 2 cut(s) 1010, 1261
BtsI GCAGTG 2 cut(s) 448, 1177
BtsIMutI CAGTG 2 cut(s) 448, 1177
BveI ACCTGC 1 cut(s) 970
Cac8I GCNNGC 3 cut(s) 337, 475, 802
CfoI GCGC 2 cut(s) 429, 1118
Cfr10I RCCGGY 2 cut(s) 245, 845
Cfr13I GGNCC 6 cut(s) 39, 657, 760, 848, 863, 1295
CseI GACGC 1 cut(s) 972
CsiI ACCWGGT 1 cut(s) 398
Csp6I GTAC 5 cut(s) 503, 529, 1026, 1075, 1207
CspCI CAANNNNNGTGG 4 cut(s) 202, 237, 594, 629
CviAII CATG 5 cut(s) 813, 878, 986, 1030, 1072
CviQI GTAC 5 cut(s) 503, 529, 1026, 1075, 1207
DpnI GATC 3 cut(s) 226, 441, 483
DpnII GATC 3 cut(s) 224, 439, 481
EaeI YGGCCR 3 cut(s) 169, 308, 735
EagI CGGCCG 1 cut(s) 735
Eam1104I CTCTTC 1 cut(s) 489
EarI CTCTTC 1 cut(s) 489
Ecl136II GAGCTC 1 cut(s) 10
EclXI CGGCCG 1 cut(s) 735
Eco130I CCWWGG 1 cut(s) 172
Eco24I GRGCYC 1 cut(s) 12
Eco47I GGWCC 3 cut(s) 39, 848, 863
Eco52I CGGCCG 1 cut(s) 735
Eco53kI GAGCTC 1 cut(s) 10
Eco57I CTGAAG 1 cut(s) 933
Eco72I CACGTG 1 cut(s) 1023
Eco91I GGTNACC 1 cut(s) 769
EcoICRI GAGCTC 1 cut(s) 10
EcoO109I RGGNCCY 1 cut(s) 863
EcoO65I GGTNACC 1 cut(s) 769
EcoRI GAATTC 1 cut(s) 553
EcoRII CCWGG 1 cut(s) 398
EcoT14I CCWWGG 1 cut(s) 172
EcoT38I GRGCYC 1 cut(s) 12
ErhI CCWWGG 1 cut(s) 172
FaeI CATG 5 cut(s) 816, 881, 989, 1033, 1075
FaqI GGGAC 5 cut(s) 52, 149, 296, 876, 927
FatI CATG 5 cut(s) 812, 877, 985, 1029, 1071
FauI CCCGC 3 cut(s) 800, 805, 853
FauNDI CATATG 1 cut(s) 952
FblI GTMKAC 1 cut(s) 1038
Fnu4HI GCNGC 4 cut(s) 323, 713, 805, 1223
FokI GGATG 2 cut(s) 997, 1248
FriOI GRGCYC 1 cut(s) 12
Fsp4HI GCNGC 4 cut(s) 323, 713, 805, 1223
GlaI GCGC 2 cut(s) 428, 1117
GluI GCNGC 4 cut(s) 323, 713, 805, 1223
HaeIII GGCC 8 cut(s) 171, 259, 310, 339, 658, 737, 762, 1296
HapII CCGG 6 cut(s) 35, 246, 533, 678, 846, 1265
HgaI GACGC 1 cut(s) 972
HhaI GCGC 2 cut(s) 429, 1118
Hin1II CATG 5 cut(s) 816, 881, 989, 1033, 1075
Hin6I GCGC 2 cut(s) 427, 1116
HinP1I GCGC 2 cut(s) 427, 1116
HincII GTYRAC 1 cut(s) 1236
HindII GTYRAC 1 cut(s) 1236
HinfI GANTC 2 cut(s) 407, 1171
HpaII CCGG 6 cut(s) 35, 246, 533, 678, 846, 1265
HphI GGTGA 2 cut(s) 88, 781
Hpy166II GTNNAC 4 cut(s) 505, 1026, 1039, 1236
Hpy188I TCNGA 2 cut(s) 353, 1137
Hpy188III TCNNGA 4 cut(s) 86, 944, 1101, 1146
Hpy8I GTNNAC 4 cut(s) 505, 1026, 1039, 1236
Hpy99I CGWCG 1 cut(s) 756
HpyAV CCTTC 6 cut(s) 582, 713, 861, 894, 1196, 1225
HpyCH4III ACNGT 4 cut(s) 115, 546, 698, 941
HpyCH4IV ACGT 2 cut(s) 386, 1022
HpyCH4V TGCA 9 cut(s) 69, 335, 550, 668, 881, 979, 985, 1111, 1154
HpyF10VI GCNNNNNNNGC 2 cut(s) 1165, 1174
HpySE526I ACGT 2 cut(s) 386, 1022
Hsp92II CATG 5 cut(s) 816, 881, 989, 1033, 1075
HspAI GCGC 2 cut(s) 427, 1116
KflI GGGWCCC 1 cut(s) 863
KpnI GGTACC 2 cut(s) 532, 1210
Kzo9I GATC 3 cut(s) 224, 439, 481
LmnI GCTCC 2 cut(s) 31, 434
Lsp1109I GCAGC 2 cut(s) 699, 1234
MabI ACCWGGT 1 cut(s) 398
MaeII ACGT 2 cut(s) 386, 1022
MaeIII GTNAC 5 cut(s) 76, 115, 263, 394, 769
MalI GATC 3 cut(s) 226, 441, 483
MboI GATC 3 cut(s) 224, 439, 481
MboII GAAGA 5 cut(s) 506, 553, 620, 681, 1119
MfeI CAATTG 1 cut(s) 120
MhlI GDGCHC 1 cut(s) 12
MlsI TGGCCA 2 cut(s) 171, 310
MluCI AATT 9 cut(s) 120, 163, 314, 365, 553, 619, 661, 685, 957
MluI ACGCGT 1 cut(s) 964
MluNI TGGCCA 2 cut(s) 171, 310
MlyI GAGTC 1 cut(s) 1165
MmeI TCCRAC 3 cut(s) 376, 581, 1249
Mox20I TGGCCA 2 cut(s) 171, 310
MscI TGGCCA 2 cut(s) 171, 310
MseI TTAA 2 cut(s) 489, 683
MslI CAYNNNNRTG 4 cut(s) 330, 380, 453, 523
Msp20I TGGCCA 2 cut(s) 171, 310
MspA1I CMGCKG 1 cut(s) 804
MspI CCGG 6 cut(s) 35, 246, 533, 678, 846, 1265
MspR9I CCNGG 4 cut(s) 36, 400, 533, 679
MunI CAATTG 1 cut(s) 120
Mva1269I GAATGC 1 cut(s) 862
MvaI CCWGG 1 cut(s) 400
MvnI CGCG 2 cut(s) 966, 1116
MwoI GCNNNNNNNGC 2 cut(s) 1165, 1174
NciI CCSGG 3 cut(s) 36, 533, 679
NdeI CATATG 1 cut(s) 952
NdeII GATC 3 cut(s) 224, 439, 481
NlaIII CATG 5 cut(s) 816, 881, 989, 1033, 1075
NlaIV GGNNCC 7 cut(s) 33, 40, 530, 864, 865, 901, 1208
NmeAIII GCCGAG 1 cut(s) 887
NmuCI GTSAC 2 cut(s) 76, 769
NspI RCATGY 2 cut(s) 816, 1075
NspV TTCGAA 1 cut(s) 854
OliI CACNNNNGTG 2 cut(s) 453, 523
PaqCI CACCTGC 1 cut(s) 970
PciI ACATGT 1 cut(s) 1071
PctI GAATGC 1 cut(s) 862
PfeI GAWTC 1 cut(s) 407
PkrI GCNGC 4 cut(s) 324, 714, 806, 1224
PleI GAGTC 1 cut(s) 1165
PmaCI CACGTG 1 cut(s) 1023
PmlI CACGTG 1 cut(s) 1023
PpsI GAGTC 1 cut(s) 1165
Ppu21I YACGTR 1 cut(s) 1023
PpuMI RGGWCCY 1 cut(s) 863
PscI ACATGT 1 cut(s) 1071
Psp124BI GAGCTC 1 cut(s) 12
Psp5II RGGWCCY 1 cut(s) 863
Psp6I CCWGG 1 cut(s) 398
PspCI CACGTG 1 cut(s) 1023
PspEI GGTNACC 1 cut(s) 769
PspGI CCWGG 1 cut(s) 398
PspN4I GGNNCC 7 cut(s) 33, 40, 530, 864, 865, 901, 1208
PspPI GGNCC 6 cut(s) 39, 657, 760, 848, 863, 1295
PspPPI RGGWCCY 1 cut(s) 863
RsaI GTAC 5 cut(s) 504, 530, 1027, 1076, 1208
RsaNI GTAC 5 cut(s) 503, 529, 1026, 1075, 1207
RseI CAYNNNNRTG 4 cut(s) 330, 380, 453, 523
SacI GAGCTC 1 cut(s) 12
SaqAI TTAA 2 cut(s) 489, 683
SatI GCNGC 4 cut(s) 323, 713, 805, 1223
Sau3AI GATC 3 cut(s) 224, 439, 481
Sau96I GGNCC 6 cut(s) 39, 657, 760, 848, 863, 1295
SchI GAGTC 1 cut(s) 1165
ScrFI CCNGG 4 cut(s) 36, 400, 533, 679
SduI GDGCHC 1 cut(s) 12
SexAI ACCWGGT 1 cut(s) 398
SfuI TTCGAA 1 cut(s) 854
SinI GGWCC 3 cut(s) 39, 848, 863
SmiMI CAYNNNNRTG 4 cut(s) 330, 380, 453, 523
Sse9I AATT 9 cut(s) 120, 163, 314, 365, 553, 619, 661, 685, 957
SspI AATATT 1 cut(s) 511
SstI GAGCTC 1 cut(s) 12
StyD4I CCNGG 4 cut(s) 34, 398, 531, 677
StyI CCWWGG 1 cut(s) 172
TaaI ACNGT 4 cut(s) 115, 546, 698, 941
TaiI ACGT 2 cut(s) 389, 1025
TaqI TCGA 5 cut(s) 87, 269, 754, 839, 854
TaqII GACCGA 1 cut(s) 56
TasI AATT 9 cut(s) 120, 163, 314, 365, 553, 619, 661, 685, 957
TatI WGTACW 2 cut(s) 502, 1074
TauI GCSGC 2 cut(s) 325, 807
TfiI GAWTC 1 cut(s) 407
Tru1I TTAA 2 cut(s) 489, 683
Tru9I TTAA 2 cut(s) 489, 683
TscAI CASTG 2 cut(s) 455, 1184
TseFI GTSAC 2 cut(s) 76, 769
TseI GCWGC 2 cut(s) 712, 1222
Tsp45I GTSAC 2 cut(s) 76, 769
TspDTI ATGAA 3 cut(s) 553, 555, 945
TspGWI ACGGA 1 cut(s) 113
TspRI CASTG 2 cut(s) 455, 1184
VpaK11BI GGWCC 3 cut(s) 39, 848, 863
XapI RAATTY 3 cut(s) 314, 553, 685
XceI RCATGY 2 cut(s) 816, 1075
XcmI CCANNNNNNNNNTGG 1 cut(s) 475
XmiI GTMKAC 1 cut(s) 1038
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.