Rh3BG264900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0003970
Physical Location & Seq
Reverse (-)
25631924 .. 25633992
2069 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG264900.1

Sequence Viewer

Length: 1299 bp
ATGGGTTTGAAATGTTTAGAGCCGATGCAATGGTTGTCCCTTGACTCCAGTCCTTCTCCATTGGGACATGCATATCCATCAAAGGTTTCCAGGAGGAATATTGGATCACTTTCAGGGCAGATGGCATTGAACAACAACAATCGTGTGAGGAGTTATGAGTTGCCTATATTCTATGCCAGCATCGACAACAATGATCCCACTGCACACCCAAGAATTCCAAGAGAGTTCAGGAAGGCAAACTTGTTTTATGACAAGTCGAAGTTAGCTTTGCTGAGTCTAAATGGGTTCCCTCAGTGGGCTTGGGAGTGTCAGGTTGTTTATTTTGAGAAGGACATATACTTCGGTACAGACTTTTATAAGTTCTGTGCTGCTGTTAGTGAAGGGAACAGAAACATCTATCTAGTTTTCAACTACGAAGGTACTCCCATGCGGTTCACGGTGACAAGATTTGATTGTAATGGACATATGCTGCGATCCCCGCCAAACCTATATTCATATCAGGGACCTCCGCTCAGTTTCGACGGTTTGGAACATCTAAAGGAGGAGGTCTTATGCGTTAACACACTTGCAATAGAGGACCACACGGACTTGCCCATGGGTAGATTTGTGGAAAAATTGGTTGCGAATGGCCTACCGGCCACATATACTATGTTGCTTGAAACCCACCCATCAATGAGTTGGAACGTTACAGTGGGTTACAATATGGACAATACCGTTGGGCTTCAGGACGGGTGGCTACCATTTGCATACGCAACCGGAATGAATGGCTCTCAATGTATTCGCTTAAAGTTACATCAAGATGGGGAAACCATTTCCGTATTGATTTTCAATGTGATCACTGGTGCAGTGACGTTACCAGATTTTGACAATCAGGGACTTGCTTACGCTATTCCTCCGCAAACCTCGGGCACCGTTCATCGTGCAATAGACATGGCCGGCAGTAGTAATCAGTCTGCAATTGATGAGGACGTAAACGACGACGAAGTTTGGCAAGGGCTTGGAGAACTTCCTTCCAGCATAAACCTAGAAGGTTTACCCAATTGCACATGCGTGATGTACCCTAGCTTCGGGAAGATGCCCCAACAGTTTGTCCGAACAGAAAAACTGAGTTCAAACTGGTACGTACTACAATCCATGGAGGGAACCGCGATGAAGGTATTTTTCAAGAAAGTCGGCAACACACTGCATCTGACAACACGGTACACGACGTTTTTGAGGGTATTTCATATTGATGCCAATCAGAAAATTTGCATGTATCTTGTCGGGCCGGATCATTTTATTTTCCACCCCACTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

432

Amino Acids

48.74

Weight (kDa)

5.65

Isoelectric Point (pI)

42.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 357
AccB1I GGYRCC 1 cut(s) 908
AccBSI CCGCTC 1 cut(s) 511
AccII CGCG 1 cut(s) 1148
AciI CCGC 5 cut(s) 430, 479, 509, 896, 1146
AclI AACGTT 1 cut(s) 684
AclWI GGATC 4 cut(s) 112, 188, 468, 1278
AcoI YGGCCR 2 cut(s) 636, 933
AcsI RAATTY 2 cut(s) 213, 1245
AcuI CTGAAG 1 cut(s) 707
AfaI GTAC 6 cut(s) 346, 421, 1058, 1121, 1125, 1202
AfiI CCNNNNNNNGG 2 cut(s) 295, 1067
AgsI TTSAA 7 cut(s) 10, 130, 409, 659, 829, 1113, 1165
AjnI CCWGG 1 cut(s) 89
AjuI GAANNNNNNNTTGG 2 cut(s) 475, 507
AleI CACNNNNGTG 1 cut(s) 1049
AluBI AGCT 2 cut(s) 266, 1065
AluI AGCT 2 cut(s) 266, 1065
AlwI GGATC 4 cut(s) 112, 188, 468, 1278
Ama87I CYCGRG 1 cut(s) 904
AoxI GGCC 4 cut(s) 628, 636, 933, 1265
ApeKI GCWGC 2 cut(s) 368, 469
ApoI RAATTY 2 cut(s) 213, 1245
ArsI GACNNNNNNTTYG 2 cut(s) 323, 355
AspS9I GGNCC 3 cut(s) 503, 577, 1265
AsuHPI GGTGA 1 cut(s) 451
AvaI CYCGRG 1 cut(s) 904
AvaII GGWCC 2 cut(s) 503, 577
BaeGI GKGCMC 1 cut(s) 911
BanI GGYRCC 1 cut(s) 908
BarI GAAGNNNNNNTAC 4 cut(s) 320, 352, 1049, 1081
BbvI GCAGC 2 cut(s) 355, 456
BccI CCATC 4 cut(s) 85, 115, 676, 794
BciT130I CCWGG 1 cut(s) 91
BclI TGATCA 1 cut(s) 834
BfaI CTAG 3 cut(s) 401, 1025, 1062
BisI GCNGC 2 cut(s) 369, 470
BlsI GCNGC 2 cut(s) 370, 471
Bme1390I CCNGG 1 cut(s) 91
Bme18I GGWCC 2 cut(s) 503, 577
BmeT110I CYCGRG 1 cut(s) 904
BmgT120I GGNCC 3 cut(s) 503, 577, 1265
BmiI GGNNCC 4 cut(s) 287, 504, 910, 1144
BmrFI CCNGG 1 cut(s) 91
BmsI GCATC 5 cut(s) 15, 189, 1065, 1195, 1222
BoxI GACNNNNGTC 1 cut(s) 48
BpmI CTGGAG 1 cut(s) 31
BsaAI YACGTR 1 cut(s) 1123
BsaBI GATNNNNATC 1 cut(s) 1236
BsaJI CCNNGG 3 cut(s) 594, 903, 1134
BsaWI WCCGGW 1 cut(s) 755
Bsc4I CCNNNNNNNGG 2 cut(s) 295, 1067
Bse118I RCCGGY 2 cut(s) 634, 935
Bse1I ACTGG 3 cut(s) 48, 844, 1121
Bse3DI GCAATG 1 cut(s) 35
Bse8I GATNNNNATC 1 cut(s) 1236
BseBI CCWGG 1 cut(s) 91
BseDI CCNNGG 3 cut(s) 594, 903, 1134
BseJI GATNNNNATC 1 cut(s) 1236
BseLI CCNNNNNNNGG 2 cut(s) 295, 1067
BseMI GCAATG 1 cut(s) 35
BseMII CTCAG 4 cut(s) 263, 305, 526, 1097
BseNI ACTGG 3 cut(s) 48, 844, 1121
BseRI GAGGAG 2 cut(s) 163, 557
BseSI GKGCMC 1 cut(s) 911
BseXI GCAGC 2 cut(s) 355, 456
BsgI GTGCAG 2 cut(s) 186, 864
Bsh1236I CGCG 1 cut(s) 1148
BshFI GGCC 4 cut(s) 630, 638, 935, 1267
BshNI GGYRCC 1 cut(s) 908
BsiHKCI CYCGRG 1 cut(s) 904
BsiSI CCGG 4 cut(s) 635, 756, 936, 1268
BslFI GGGAC 4 cut(s) 22, 78, 516, 888
BslI CCNNNNNNNGG 2 cut(s) 295, 1067
BsmFI GGGAC 4 cut(s) 22, 78, 516, 888
BsnI GGCC 4 cut(s) 630, 638, 935, 1267
BsoBI CYCGRG 1 cut(s) 904
Bsp1286I GDGCHC 1 cut(s) 911
Bsp143I GATC 5 cut(s) 104, 193, 473, 834, 1270
Bsp19I CCATGG 2 cut(s) 594, 1134
BspACI CCGC 5 cut(s) 430, 479, 509, 896, 1146
BspANI GGCC 4 cut(s) 630, 638, 935, 1267
BspCNI CTCAG 4 cut(s) 264, 304, 525, 1098
BspFNI CGCG 1 cut(s) 1148
BspLI GGNNCC 4 cut(s) 287, 504, 910, 1144
BspPI GGATC 4 cut(s) 112, 188, 468, 1278
BspT107I GGYRCC 1 cut(s) 908
BsrBI CCGCTC 1 cut(s) 511
BsrDI GCAATG 1 cut(s) 35
BsrFI RCCGGY 2 cut(s) 634, 935
BsrI ACTGG 3 cut(s) 48, 844, 1121
BssAI RCCGGY 2 cut(s) 634, 935
BssECI CCNNGG 3 cut(s) 594, 903, 1134
BssMI GATC 5 cut(s) 104, 193, 473, 834, 1270
BssT1I CCWWGG 2 cut(s) 594, 1134
Bst2UI CCWGG 1 cut(s) 91
Bst4CI ACNGT 7 cut(s) 439, 524, 691, 715, 913, 1086, 1200
BstBAI YACGTR 1 cut(s) 1123
BstC8I GCNNGC 2 cut(s) 178, 937
BstDEI CTNAG 4 cut(s) 272, 291, 512, 1106
BstDSI CCRYGG 2 cut(s) 594, 1134
BstFNI CGCG 1 cut(s) 1148
BstKTI GATC 5 cut(s) 107, 196, 476, 837, 1273
BstMBI GATC 5 cut(s) 104, 193, 473, 834, 1270
BstMWI GCNNNNNNNGC 1 cut(s) 478
BstNI CCWGG 1 cut(s) 91
BstNSI RCATGY 3 cut(s) 71, 1050, 1255
BstPAI GACNNNNGTC 1 cut(s) 48
BstSCI CCNGG 1 cut(s) 89
BstSLI GKGCMC 1 cut(s) 911
BstSNI TACGTA 1 cut(s) 1123
BstUI CGCG 1 cut(s) 1148
BstV1I GCAGC 2 cut(s) 355, 456
BsuRI GGCC 4 cut(s) 630, 638, 935, 1267
BtgI CCRYGG 2 cut(s) 594, 1134
BtgZI GCGATG 1 cut(s) 1163
BtsI GCAGTG 3 cut(s) 198, 852, 1181
BtsIMutI CAGTG 7 cut(s) 198, 299, 696, 837, 852, 1181, 1290
Cac8I GCNNGC 2 cut(s) 178, 937
Cfr10I RCCGGY 2 cut(s) 634, 935
Cfr13I GGNCC 3 cut(s) 503, 577, 1265
Csp6I GTAC 6 cut(s) 345, 420, 1057, 1120, 1124, 1201
CviAII CATG 7 cut(s) 68, 427, 595, 931, 1047, 1135, 1252
CviQI GTAC 6 cut(s) 345, 420, 1057, 1120, 1124, 1201
DdeI CTNAG 4 cut(s) 272, 291, 512, 1106
DpnI GATC 5 cut(s) 106, 195, 475, 836, 1272
DpnII GATC 5 cut(s) 104, 193, 473, 834, 1270
EaeI YGGCCR 2 cut(s) 636, 933
Eco105I TACGTA 1 cut(s) 1123
Eco130I CCWWGG 2 cut(s) 594, 1134
Eco47I GGWCC 2 cut(s) 503, 577
Eco57I CTGAAG 1 cut(s) 707
Eco88I CYCGRG 1 cut(s) 904
EcoO109I RGGNCCY 1 cut(s) 503
EcoRI GAATTC 1 cut(s) 213
EcoRII CCWGG 1 cut(s) 89
EcoT14I CCWWGG 2 cut(s) 594, 1134
EcoT22I ATGCAT 1 cut(s) 73
ErhI CCWWGG 2 cut(s) 594, 1134
FaeI CATG 7 cut(s) 71, 430, 598, 934, 1050, 1138, 1255
FalI AAGNNNNNCTT 2 cut(s) 224, 256
FaqI GGGAC 4 cut(s) 22, 78, 516, 888
FatI CATG 7 cut(s) 67, 426, 594, 930, 1046, 1134, 1251
FauI CCCGC 1 cut(s) 486
FauNDI CATATG 1 cut(s) 465
FbaI TGATCA 1 cut(s) 834
Fnu4HI GCNGC 2 cut(s) 369, 470
Fsp4HI GCNGC 2 cut(s) 369, 470
FspBI CTAG 3 cut(s) 401, 1025, 1062
GluI GCNGC 2 cut(s) 369, 470
GsuI CTGGAG 1 cut(s) 31
HaeIII GGCC 4 cut(s) 630, 638, 935, 1267
HapII CCGG 4 cut(s) 635, 756, 936, 1268
Hin1II CATG 7 cut(s) 71, 430, 598, 934, 1050, 1138, 1255
HincII GTYRAC 1 cut(s) 559
HindII GTYRAC 1 cut(s) 559
HinfI GANTC 2 cut(s) 44, 274
HpaI GTTAAC 1 cut(s) 559
HpaII CCGG 4 cut(s) 635, 756, 936, 1268
HphI GGTGA 1 cut(s) 451
Hpy166II GTNNAC 5 cut(s) 435, 559, 973, 1034, 1203
Hpy188I TCNGA 3 cut(s) 1094, 1191, 1242
Hpy188III TCNNGA 5 cut(s) 229, 725, 797, 1069, 1165
Hpy8I GTNNAC 5 cut(s) 435, 559, 973, 1034, 1203
Hpy99I CGWCG 4 cut(s) 524, 980, 983, 1210
HpyAV CCTTC 8 cut(s) 63, 226, 322, 374, 410, 1020, 1022, 1147
HpyCH4III ACNGT 7 cut(s) 439, 524, 691, 715, 913, 1086, 1200
HpyCH4IV ACGT 5 cut(s) 684, 851, 969, 1122, 1208
HpyF10VI GCNNNNNNNGC 1 cut(s) 478
HpyF3I CTNAG 4 cut(s) 272, 291, 512, 1106
HpySE526I ACGT 5 cut(s) 684, 851, 969, 1122, 1208
Hsp92II CATG 7 cut(s) 71, 430, 598, 934, 1050, 1138, 1255
KroI GCCGGC 1 cut(s) 935
KroNI GCCGGC 1 cut(s) 937
Ksp22I TGATCA 1 cut(s) 834
KspAI GTTAAC 1 cut(s) 559
Kzo9I GATC 5 cut(s) 104, 193, 473, 834, 1270
Lsp1109I GCAGC 2 cut(s) 355, 456
LweI GCATC 5 cut(s) 15, 189, 1065, 1195, 1222
MaeI CTAG 3 cut(s) 401, 1025, 1062
MaeII ACGT 5 cut(s) 684, 851, 969, 1122, 1208
MaeIII GTNAC 6 cut(s) 439, 685, 695, 789, 847, 852
MalI GATC 5 cut(s) 106, 195, 475, 836, 1272
MbiI CCGCTC 1 cut(s) 511
MboI GATC 5 cut(s) 104, 193, 473, 834, 1270
MboII GAAGA 1 cut(s) 1084
MfeI CAATTG 2 cut(s) 957, 1039
MhlI GDGCHC 1 cut(s) 911
MluCI AATT 5 cut(s) 213, 614, 957, 1039, 1245
MlyI GAGTC 2 cut(s) 38, 283
MmeI TCCRAC 1 cut(s) 659
Mph1103I ATGCAT 1 cut(s) 73
MroNI GCCGGC 1 cut(s) 935
MseI TTAA 3 cut(s) 558, 785, 1297
MslI CAYNNNNRTG 3 cut(s) 798, 1049, 1230
MspI CCGG 4 cut(s) 635, 756, 936, 1268
MspR9I CCNGG 1 cut(s) 91
MunI CAATTG 2 cut(s) 957, 1039
MvaI CCWGG 1 cut(s) 91
MvnI CGCG 1 cut(s) 1148
MwoI GCNNNNNNNGC 1 cut(s) 478
NaeI GCCGGC 1 cut(s) 937
NcoI CCATGG 2 cut(s) 594, 1134
NdeI CATATG 1 cut(s) 465
NdeII GATC 5 cut(s) 104, 193, 473, 834, 1270
NgoMIV GCCGGC 1 cut(s) 935
NlaIII CATG 7 cut(s) 71, 430, 598, 934, 1050, 1138, 1255
NlaIV GGNNCC 4 cut(s) 287, 504, 910, 1144
NmuCI GTSAC 2 cut(s) 439, 847
NsiI ATGCAT 1 cut(s) 73
NspI RCATGY 3 cut(s) 71, 1050, 1255
OliI CACNNNNGTG 1 cut(s) 1049
PcsI WCGNNNNNNNCGW 1 cut(s) 975
PdiI GCCGGC 1 cut(s) 937
PfoI TCCNGGA 1 cut(s) 89
PkrI GCNGC 2 cut(s) 370, 471
PleI GAGTC 2 cut(s) 38, 282
PpsI GAGTC 2 cut(s) 38, 282
Ppu21I YACGTR 1 cut(s) 1123
PpuMI RGGWCCY 1 cut(s) 503
PshAI GACNNNNGTC 1 cut(s) 48
PsiI TTATAA 1 cut(s) 357
Psp1406I AACGTT 1 cut(s) 684
Psp5II RGGWCCY 1 cut(s) 503
Psp6I CCWGG 1 cut(s) 89
PspGI CCWGG 1 cut(s) 89
PspN4I GGNNCC 4 cut(s) 287, 504, 910, 1144
PspPI GGNCC 3 cut(s) 503, 577, 1265
PspPPI RGGWCCY 1 cut(s) 503
RsaI GTAC 6 cut(s) 346, 421, 1058, 1121, 1125, 1202
RsaNI GTAC 6 cut(s) 345, 420, 1057, 1120, 1124, 1201
RseI CAYNNNNRTG 3 cut(s) 798, 1049, 1230
SaqAI TTAA 3 cut(s) 558, 785, 1297
SatI GCNGC 2 cut(s) 369, 470
Sau3AI GATC 5 cut(s) 104, 193, 473, 834, 1270
Sau96I GGNCC 3 cut(s) 503, 577, 1265
SchI GAGTC 2 cut(s) 38, 283
ScrFI CCNGG 1 cut(s) 91
SduI GDGCHC 1 cut(s) 911
SfaNI GCATC 5 cut(s) 15, 189, 1065, 1195, 1222
SinI GGWCC 2 cut(s) 503, 577
SmiMI CAYNNNNRTG 3 cut(s) 798, 1049, 1230
SnaBI TACGTA 1 cut(s) 1123
Sse9I AATT 5 cut(s) 213, 614, 957, 1039, 1245
SsiI CCGC 5 cut(s) 430, 479, 509, 896, 1146
SspI AATATT 1 cut(s) 100
SspMI CTAG 3 cut(s) 401, 1025, 1062
StyD4I CCNGG 1 cut(s) 89
StyI CCWWGG 2 cut(s) 594, 1134
TaaI ACNGT 7 cut(s) 439, 524, 691, 715, 913, 1086, 1200
TaiI ACGT 5 cut(s) 687, 854, 972, 1125, 1211
TaqI TCGA 3 cut(s) 183, 257, 519
TasI AATT 5 cut(s) 213, 614, 957, 1039, 1245
Tru1I TTAA 3 cut(s) 558, 785, 1297
Tru9I TTAA 3 cut(s) 558, 785, 1297
TscAI CASTG 7 cut(s) 205, 299, 696, 844, 852, 1188, 1297
TseFI GTSAC 2 cut(s) 439, 847
TseI GCWGC 2 cut(s) 368, 469
Tsp45I GTSAC 2 cut(s) 439, 847
TspDTI ATGAA 5 cut(s) 483, 776, 905, 1166, 1214
TspGWI ACGGA 2 cut(s) 599, 805
TspRI CASTG 7 cut(s) 205, 299, 696, 844, 852, 1188, 1297
VpaK11BI GGWCC 2 cut(s) 503, 577
XapI RAATTY 2 cut(s) 213, 1245
XceI RCATGY 3 cut(s) 71, 1050, 1255
XcmI CCANNNNNNNNNTGG 1 cut(s) 675
XspI CTAG 3 cut(s) 401, 1025, 1062
Zsp2I ATGCAT 1 cut(s) 73
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.