Rh4BG133000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0003970
Physical Location & Seq
Reverse (-)
22406040 .. 22406692
653 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG133000.1

Sequence Viewer

Length: 540 bp
ATGTCCGTATTGATGTTCAATGCATACACTGGTGCAGTGAAGTTGTCAGATTTTGAAGGGGCGGGACCTGTTAACGCGATTTCGTCGCAAACCCCGACCACGGTTCACCGTGCAATAGACATGGCTGACAGTAGTAATGAGGCTACAATTGATGAGAACGTCAACAACGACGAAGTTTGGCAAGGGCTTGGAGAACTTCCTTCGAGCCAAAACCTACAAGATTTTCCCAATTGCACATGCGTGATGTACTCTAGCTTCGGGGTTAGTCATCGTGTTGTTATGTGCACCAATATCATGAAACATGCGCAAACAGCAATGCCCCAGCAGTTTGCTCGTAAAGAAAAACTGAAGGCGAACTGGTACATACTACAATCCATGGAGAGAACCGACATGAAGGTATTTTTCAAGAAAGTCGGCAAGACACTACATCTGACAATACGGTACATGAAGTTCTTGAGGGTATTTGACATTGATCCCGATCAGAGAATGCACATGTATCTTGTCGGGCCAGATCAATCCATTTTTCACCTCACTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

179

Amino Acids

20.38

Weight (kDa)

6.29

Isoelectric Point (pI)

44.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 306
AccII CGCG 1 cut(s) 77
AciI CCGC 1 cut(s) 62
AclWI GGATC 1 cut(s) 467
AcuI CTGAAG 1 cut(s) 368
AfaI GTAC 3 cut(s) 248, 362, 443
AfiI CCNNNNNNNGG 1 cut(s) 100
AflIII ACRYGT 1 cut(s) 492
AgsI TTSAA 3 cut(s) 19, 56, 406
AleI CACNNNNGTG 1 cut(s) 239
AluBI AGCT 1 cut(s) 255
AluI AGCT 1 cut(s) 255
Alw21I GWGCWC 1 cut(s) 287
Alw44I GTGCAC 1 cut(s) 283
AlwI GGATC 1 cut(s) 467
AoxI GGCC 1 cut(s) 506
ApaLI GTGCAC 1 cut(s) 283
AspLEI GCGC 1 cut(s) 307
AspS9I GGNCC 2 cut(s) 65, 506
AsuHPI GGTGA 2 cut(s) 98, 518
AvaII GGWCC 1 cut(s) 65
BaeGI GKGCMC 1 cut(s) 287
BarI GAAGNNNNNNTAC 2 cut(s) 239, 271
Bbv12I GWGCWC 1 cut(s) 287
BcgI CGANNNNNNTGC 2 cut(s) 314, 348
BfaI CTAG 1 cut(s) 252
Bme18I GGWCC 1 cut(s) 65
BmgT120I GGNCC 2 cut(s) 65, 506
BmiI GGNNCC 1 cut(s) 66
BpuEI CTTGAG 1 cut(s) 475
BsaBI GATNNNNATC 1 cut(s) 477
BsaJI CCNNGG 2 cut(s) 99, 375
Bsc4I CCNNNNNNNGG 1 cut(s) 100
Bse1I ACTGG 2 cut(s) 34, 362
Bse3DI GCAATG 1 cut(s) 321
Bse8I GATNNNNATC 1 cut(s) 477
BseDI CCNNGG 2 cut(s) 99, 375
BseJI GATNNNNATC 1 cut(s) 477
BseLI CCNNNNNNNGG 1 cut(s) 100
BseMI GCAATG 1 cut(s) 321
BseNI ACTGG 2 cut(s) 34, 362
BseSI GKGCMC 1 cut(s) 287
BseYI CCCAGC 1 cut(s) 321
BsgI GTGCAG 1 cut(s) 54
Bsh1236I CGCG 1 cut(s) 77
BshFI GGCC 1 cut(s) 508
BsiHKAI GWGCWC 1 cut(s) 287
BslFI GGGAC 1 cut(s) 78
BslI CCNNNNNNNGG 1 cut(s) 100
BsmFI GGGAC 1 cut(s) 78
BsmI GAATGC 1 cut(s) 492
BsnI GGCC 1 cut(s) 508
Bsp1286I GDGCHC 1 cut(s) 287
Bsp143I GATC 3 cut(s) 472, 478, 511
Bsp19I CCATGG 1 cut(s) 375
BspACI CCGC 1 cut(s) 62
BspANI GGCC 1 cut(s) 508
BspFNI CGCG 1 cut(s) 77
BspHI TCATGA 1 cut(s) 294
BspLI GGNNCC 1 cut(s) 66
BspPI GGATC 1 cut(s) 467
BsrDI GCAATG 1 cut(s) 321
BsrI ACTGG 2 cut(s) 34, 362
BssECI CCNNGG 2 cut(s) 99, 375
BssMI GATC 3 cut(s) 472, 478, 511
BssT1I CCWWGG 1 cut(s) 375
Bst4CI ACNGT 4 cut(s) 103, 110, 131, 441
BstDSI CCRYGG 2 cut(s) 99, 375
BstFNI CGCG 1 cut(s) 77
BstHHI GCGC 1 cut(s) 307
BstKTI GATC 3 cut(s) 475, 481, 514
BstMBI GATC 3 cut(s) 472, 478, 511
BstMWI GCNNNNNNNGC 1 cut(s) 311
BstNSI RCATGY 3 cut(s) 240, 305, 496
BstSLI GKGCMC 1 cut(s) 287
BstUI CGCG 1 cut(s) 77
BsuRI GGCC 1 cut(s) 508
BtgI CCRYGG 2 cut(s) 99, 375
BtsI GCAGTG 1 cut(s) 42
BtsIMutI CAGTG 3 cut(s) 27, 42, 531
CciI TCATGA 1 cut(s) 294
CfoI GCGC 1 cut(s) 307
Cfr13I GGNCC 2 cut(s) 65, 506
Csp6I GTAC 3 cut(s) 247, 361, 442
CviAII CATG 8 cut(s) 121, 237, 295, 302, 376, 391, 445, 493
CviJI RGCY 6 cut(s) 125, 143, 187, 207, 255, 508
CviKI_1 RGCY 6 cut(s) 125, 143, 187, 207, 255, 508
CviQI GTAC 3 cut(s) 247, 361, 442
DpnI GATC 3 cut(s) 474, 480, 513
DpnII GATC 3 cut(s) 472, 478, 511
Eco130I CCWWGG 1 cut(s) 375
Eco47I GGWCC 1 cut(s) 65
Eco57I CTGAAG 1 cut(s) 368
EcoO109I RGGNCCY 1 cut(s) 65
EcoT14I CCWWGG 1 cut(s) 375
EcoT22I ATGCAT 1 cut(s) 25
ErhI CCWWGG 1 cut(s) 375
FaeI CATG 8 cut(s) 124, 240, 298, 305, 379, 394, 448, 496
FaqI GGGAC 1 cut(s) 78
FatI CATG 8 cut(s) 120, 236, 294, 301, 375, 390, 444, 492
FauI CCCGC 1 cut(s) 55
FspBI CTAG 1 cut(s) 252
FspI TGCGCA 1 cut(s) 306
GlaI GCGC 1 cut(s) 306
GsaI CCCAGC 1 cut(s) 325
HaeIII GGCC 1 cut(s) 508
HhaI GCGC 1 cut(s) 307
Hin1II CATG 8 cut(s) 124, 240, 298, 305, 379, 394, 448, 496
Hin6I GCGC 1 cut(s) 305
HinP1I GCGC 1 cut(s) 305
HincII GTYRAC 2 cut(s) 73, 163
HindII GTYRAC 2 cut(s) 73, 163
HpaI GTTAAC 1 cut(s) 73
HphI GGTGA 2 cut(s) 98, 518
Hpy166II GTNNAC 4 cut(s) 73, 106, 163, 285
Hpy188I TCNGA 3 cut(s) 49, 432, 483
Hpy188III TCNNGA 4 cut(s) 295, 406, 454, 476
Hpy8I GTNNAC 4 cut(s) 73, 106, 163, 285
Hpy99I CGWCG 2 cut(s) 88, 173
HpyAV CCTTC 4 cut(s) 50, 210, 343, 388
HpyCH4III ACNGT 4 cut(s) 103, 110, 131, 441
HpyCH4IV ACGT 1 cut(s) 159
HpyCH4V TGCA 6 cut(s) 23, 35, 113, 234, 285, 490
HpyF10VI GCNNNNNNNGC 1 cut(s) 311
HpySE526I ACGT 1 cut(s) 159
Hsp92II CATG 8 cut(s) 124, 240, 298, 305, 379, 394, 448, 496
HspAI GCGC 1 cut(s) 305
KspAI GTTAAC 1 cut(s) 73
Kzo9I GATC 3 cut(s) 472, 478, 511
LpnPI CCDG 5 cut(s) 15, 81, 335, 343, 522
MaeI CTAG 1 cut(s) 252
MaeII ACGT 1 cut(s) 159
MalI GATC 3 cut(s) 474, 480, 513
MboI GATC 3 cut(s) 472, 478, 511
MfeI CAATTG 2 cut(s) 147, 229
MhlI GDGCHC 1 cut(s) 287
MluCI AATT 2 cut(s) 147, 229
MnlI CCTC 3 cut(s) 133, 450, 539
Mph1103I ATGCAT 1 cut(s) 25
MseI TTAA 2 cut(s) 72, 538
MslI CAYNNNNRTG 1 cut(s) 239
MunI CAATTG 2 cut(s) 147, 229
Mva1269I GAATGC 1 cut(s) 492
MvnI CGCG 1 cut(s) 77
MwoI GCNNNNNNNGC 1 cut(s) 311
NcoI CCATGG 1 cut(s) 375
NdeII GATC 3 cut(s) 472, 478, 511
NlaIII CATG 8 cut(s) 124, 240, 298, 305, 379, 394, 448, 496
NlaIV GGNNCC 1 cut(s) 66
NsbI TGCGCA 1 cut(s) 306
NsiI ATGCAT 1 cut(s) 25
NspI RCATGY 3 cut(s) 240, 305, 496
OliI CACNNNNGTG 1 cut(s) 239
PagI TCATGA 1 cut(s) 294
PciI ACATGT 1 cut(s) 492
PcsI WCGNNNNNNNCGW 2 cut(s) 92, 165
PctI GAATGC 1 cut(s) 492
PpuMI RGGWCCY 1 cut(s) 65
PscI ACATGT 1 cut(s) 492
Psp5II RGGWCCY 1 cut(s) 65
PspFI CCCAGC 1 cut(s) 321
PspN4I GGNNCC 1 cut(s) 66
PspPI GGNCC 2 cut(s) 65, 506
PspPPI RGGWCCY 1 cut(s) 65
PsrI GAACNNNNNNTAC 2 cut(s) 434, 466
RsaI GTAC 3 cut(s) 248, 362, 443
RsaNI GTAC 3 cut(s) 247, 361, 442
RseI CAYNNNNRTG 1 cut(s) 239
SaqAI TTAA 2 cut(s) 72, 538
Sau3AI GATC 3 cut(s) 472, 478, 511
Sau96I GGNCC 2 cut(s) 65, 506
SduI GDGCHC 1 cut(s) 287
SetI ASST 6 cut(s) 70, 162, 216, 257, 399, 531
SinI GGWCC 1 cut(s) 65
SmiMI CAYNNNNRTG 1 cut(s) 239
SmlI CTYRAG 1 cut(s) 454
SmoI CTYRAG 1 cut(s) 454
Sse9I AATT 2 cut(s) 147, 229
SsiI CCGC 1 cut(s) 62
SspMI CTAG 1 cut(s) 252
StyI CCWWGG 1 cut(s) 375
TaaI ACNGT 4 cut(s) 103, 110, 131, 441
TaiI ACGT 1 cut(s) 162
TaqI TCGA 1 cut(s) 203
TasI AATT 2 cut(s) 147, 229
TatI WGTACW 1 cut(s) 246
Tru1I TTAA 2 cut(s) 72, 538
Tru9I TTAA 2 cut(s) 72, 538
TscAI CASTG 3 cut(s) 34, 42, 538
TspDTI ATGAA 3 cut(s) 311, 407, 461
TspRI CASTG 3 cut(s) 34, 42, 538
VneI GTGCAC 1 cut(s) 283
VpaK11BI GGWCC 1 cut(s) 65
XceI RCATGY 3 cut(s) 240, 305, 496
XspI CTAG 1 cut(s) 252
Zsp2I ATGCAT 1 cut(s) 25
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.