Rmu_sc0006198.1_g000015

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006198.1
OG0002876
Physical Location & Seq
Reverse (-)
65025 .. 67538
2514 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1104 bp
atggcatcagcaagtggatcaacccctgaacttcgaatgggaatgcagccgtgtttcatccaggtcgtgaggacaccgtccaagaacatagacagtttggtgatccctcctccatttgtagaagaattcatgaatggcgacagatgccgcaaagggaaaattatactggacgggtgccccgtacttcaatgggtggttgaaataaatacggatggtaatggggtggaattgggcgatgggtgggcgcaatttgttgcccaaaatatgaccgaaggctggatgctagtgatgttaaggtacgagggggcaatgatattttatgcaaggaggtatggcccttcaggagcgctgatggtggggacgccaaatgggacaattgtgaagaaaacatgtgcttcaggtgggggcgttttaccatcatttgcccggttggtgattcccgatggagtgaacagccaggaattggacatacccccggggatattacgtggaacagcaggtcggaacaggattgacagtaaaatatgggtgaacaatatgaactggggaatgtcgtgcaacgcacgtatccagctaagcgaggaaggaggagtaataaaaggaggttgggaaatgtttgtgagggcaaacattagccgggcatccaagtggtgcattgtggatgtagatcgtttcaagcaggaggtaagagtgatggcatttgtgcaatgcgggggggtgatggcgtcgagggcaatgatgagtgtccaaacaataactgatagactgtacgttgatagtgctgaggaagactaccctgacacaaatgacaatgatgagggagatgattcgtcggatgaagaacaagatgctgagttgcaacaaaatccaatcccaagtgttgtggtcagagtgggagagaaatatgcggttcagcggccgtttcgtggaactgaggacagcatatggccttacagtgggaaacaaatgcaaggtgacttacctgggaggctgggtgttcttcatggagagtctgtgagcttggtggcagttaagatgagattggggaagggcgatggtgaaggagacatgatcatgttaacttttgatggttgtaggacttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

367

Amino Acids

40.25

Weight (kDa)

4.89

Isoelectric Point (pI)

38.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 20 - 102 3.8e-06 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 488
AccB1I GGYRCC 1 cut(s) 174
AccB7I CCANNNNNTGG 1 cut(s) 463
AciI CCGC 4 cut(s) 148, 711, 908, 916
AclWI GGATC 2 cut(s) 25, 97
AcoI YGGCCR 1 cut(s) 917
AcsI RAATTY 1 cut(s) 125
AcuI CTGAAG 2 cut(s) 324, 381
AcyI GRCGYC 2 cut(s) 362, 725
AfaI GTAC 3 cut(s) 183, 299, 770
AfeI AGCGCT 1 cut(s) 348
AfiI CCNNNNNNNGG 4 cut(s) 276, 463, 926, 956
AflIII ACRYGT 1 cut(s) 389
AgsI TTSAA 3 cut(s) 188, 200, 676
AjnI CCWGG 3 cut(s) 60, 456, 982
AluBI AGCT 2 cut(s) 574, 1020
AluI AGCT 2 cut(s) 574, 1020
Alw26I GTCTC 1 cut(s) 1059
AlwI GGATC 2 cut(s) 25, 97
Ama87I CYCGRG 1 cut(s) 475
Aor51HI AGCGCT 1 cut(s) 348
AoxI GGCC 3 cut(s) 334, 917, 947
ApeKI GCWGC 1 cut(s) 46
ApoI RAATTY 1 cut(s) 125
AspLEI GCGC 2 cut(s) 247, 349
AspS9I GGNCC 1 cut(s) 335
AsuC2I CCSGG 4 cut(s) 427, 476, 477, 638
AsuHPI GGTGA 6 cut(s) 112, 445, 541, 730, 986, 1070
AsuII TTCGAA 1 cut(s) 34
AvaI CYCGRG 1 cut(s) 475
BaeGI GKGCMC 1 cut(s) 179
BaeI ACNNNNGTAYC 2 cut(s) 550, 583
BanI GGYRCC 1 cut(s) 174
BbsI GAAGAC 1 cut(s) 795
BbvCI CCTCAGC 1 cut(s) 783
BbvI GCAGC 1 cut(s) 58
BccI CCATC 9 cut(s) 206, 230, 346, 424, 437, 688, 715, 1049, 1082
BceAI ACGGC 2 cut(s) 34, 904
BciT130I CCWGG 3 cut(s) 62, 458, 984
BciVI GTATCC 1 cut(s) 578
BclI TGATCA 1 cut(s) 1071
BcnI CCSGG 4 cut(s) 427, 476, 477, 638
BcoDI GTCTC 1 cut(s) 1059
BfaI CTAG 1 cut(s) 284
BfoI RGCGCY 1 cut(s) 350
BfuAI ACCTGC 1 cut(s) 488
BfuI GTATCC 1 cut(s) 578
BisI GCNGC 3 cut(s) 47, 148, 917
BlpI GCTNAGC 1 cut(s) 575
BlsI GCNGC 3 cut(s) 48, 149, 918
Bme1390I CCNGG 7 cut(s) 62, 427, 458, 476, 477, 638, 984
BmeT110I CYCGRG 1 cut(s) 475
BmgT120I GGNCC 1 cut(s) 335
BmiI GGNNCC 1 cut(s) 176
BmrFI CCNGG 7 cut(s) 62, 427, 458, 476, 477, 638, 984
BmrI ACTGGG 1 cut(s) 553
BmsI GCATC 5 cut(s) 14, 134, 270, 650, 838
BmuI ACTGGG 1 cut(s) 553
BpiI GAAGAC 1 cut(s) 795
Bpu10I CCTNAGC 1 cut(s) 783
Bpu1102I GCTNAGC 1 cut(s) 575
Bpu14I TTCGAA 1 cut(s) 34
BpuMI CCSGG 4 cut(s) 427, 476, 477, 638
BsaAI YACGTR 2 cut(s) 488, 566
BsaBI GATNNNNATC 1 cut(s) 666
BsaHI GRCGYC 2 cut(s) 362, 725
BsaJI CCNNGG 4 cut(s) 474, 475, 476, 983
BsaXI ACNNNNNCTCC 4 cut(s) 979, 999, 1009, 1029
Bsc4I CCNNNNNNNGG 4 cut(s) 276, 463, 926, 956
Bse1I ACTGG 2 cut(s) 171, 548
Bse3DI GCAATG 3 cut(s) 315, 713, 741
Bse8I GATNNNNATC 1 cut(s) 666
BseBI CCWGG 3 cut(s) 62, 458, 984
BseDI CCNNGG 4 cut(s) 474, 475, 476, 983
BseGI GGATG 6 cut(s) 57, 217, 285, 641, 667, 841
BseJI GATNNNNATC 1 cut(s) 666
BseLI CCNNNNNNNGG 4 cut(s) 276, 463, 926, 956
BseMI GCAATG 3 cut(s) 315, 713, 741
BseMII CTCAG 3 cut(s) 774, 843, 924
BseNI ACTGG 2 cut(s) 171, 548
BseRI GAGGAG 2 cut(s) 99, 603
BseSI GKGCMC 1 cut(s) 179
BseX3I CGGCCG 1 cut(s) 917
BseXI GCAGC 1 cut(s) 58
BseYI CCCAGC 1 cut(s) 991
Bsh1285I CGRYCG 1 cut(s) 920
BshFI GGCC 3 cut(s) 336, 919, 949
BshNI GGYRCC 1 cut(s) 174
BsiEI CGRYCG 1 cut(s) 920
BsiHKCI CYCGRG 1 cut(s) 475
BsiSI CCGG 3 cut(s) 427, 476, 637
BslFI GGGAC 2 cut(s) 373, 385
BslI CCNNNNNNNGG 4 cut(s) 276, 463, 926, 956
BsmAI GTCTC 1 cut(s) 1059
BsmFI GGGAC 2 cut(s) 373, 385
BsmI GAATGC 1 cut(s) 48
BsnI GGCC 3 cut(s) 336, 919, 949
BsoBI CYCGRG 1 cut(s) 475
Bsp119I TTCGAA 1 cut(s) 34
Bsp1286I GDGCHC 1 cut(s) 179
Bsp143I GATC 4 cut(s) 17, 102, 667, 1071
Bsp1720I GCTNAGC 1 cut(s) 575
BspACI CCGC 4 cut(s) 148, 711, 908, 916
BspANI GGCC 3 cut(s) 336, 919, 949
BspCNI CTCAG 3 cut(s) 775, 844, 925
BspHI TCATGA 1 cut(s) 129
BspLI GGNNCC 1 cut(s) 176
BspMI ACCTGC 1 cut(s) 488
BspPI GGATC 2 cut(s) 25, 97
BspT104I TTCGAA 1 cut(s) 34
BspT107I GGYRCC 1 cut(s) 174
BsrDI GCAATG 3 cut(s) 315, 713, 741
BsrI ACTGG 2 cut(s) 171, 548
BssECI CCNNGG 4 cut(s) 474, 475, 476, 983
BssMI GATC 4 cut(s) 17, 102, 667, 1071
BssNI GRCGYC 2 cut(s) 362, 725
Bst2UI CCWGG 3 cut(s) 62, 458, 984
Bst4CI ACNGT 5 cut(s) 78, 95, 518, 768, 956
BstACI GRCGYC 2 cut(s) 362, 725
BstBAI YACGTR 2 cut(s) 488, 566
BstBI TTCGAA 1 cut(s) 34
BstDEI CTNAG 4 cut(s) 575, 783, 852, 933
BstF5I GGATG 6 cut(s) 57, 217, 285, 641, 667, 841
BstH2I RGCGCY 1 cut(s) 350
BstHHI GCGC 2 cut(s) 247, 349
BstKTI GATC 4 cut(s) 20, 105, 670, 1074
BstMAI GTCTC 1 cut(s) 1059
BstMBI GATC 4 cut(s) 17, 102, 667, 1071
BstMCI CGRYCG 1 cut(s) 920
BstMWI GCNNNNNNNGC 2 cut(s) 144, 731
BstNI CCWGG 3 cut(s) 62, 458, 984
BstNSI RCATGY 1 cut(s) 393
BstSCI CCNGG 7 cut(s) 60, 425, 456, 474, 475, 636, 982
BstSLI GKGCMC 1 cut(s) 179
BstV1I GCAGC 1 cut(s) 58
BstV2I GAAGAC 1 cut(s) 795
BstZI CGGCCG 1 cut(s) 917
BsuI GTATCC 1 cut(s) 578
BsuRI GGCC 3 cut(s) 336, 919, 949
BtgZI GCGATG 2 cut(s) 249, 1068
BtsCI GGATG 6 cut(s) 57, 217, 285, 641, 667, 841
BtsIMutI CAGTG 1 cut(s) 961
BveI ACCTGC 1 cut(s) 488
CciI TCATGA 1 cut(s) 129
CfoI GCGC 2 cut(s) 247, 349
Cfr13I GGNCC 1 cut(s) 335
Cfr9I CCCGGG 1 cut(s) 475
CseI GACGC 2 cut(s) 370, 714
Csp6I GTAC 3 cut(s) 182, 298, 769
CspCI CAANNNNNGTGG 2 cut(s) 864, 899
CviAII CATG 5 cut(s) 130, 390, 1004, 1069, 1075
CviQI GTAC 3 cut(s) 182, 298, 769
DdeI CTNAG 4 cut(s) 575, 783, 852, 933
DpnI GATC 4 cut(s) 19, 104, 669, 1073
DpnII GATC 4 cut(s) 17, 102, 667, 1071
EaeI YGGCCR 1 cut(s) 917
EagI CGGCCG 1 cut(s) 917
EclXI CGGCCG 1 cut(s) 917
Eco47III AGCGCT 1 cut(s) 348
Eco52I CGGCCG 1 cut(s) 917
Eco57I CTGAAG 2 cut(s) 324, 381
Eco88I CYCGRG 1 cut(s) 475
EcoRI GAATTC 1 cut(s) 125
EcoRII CCWGG 3 cut(s) 60, 456, 982
FaeI CATG 5 cut(s) 133, 393, 1007, 1072, 1078
FaqI GGGAC 2 cut(s) 373, 385
FatI CATG 5 cut(s) 129, 389, 1003, 1068, 1074
FauI CCCGC 1 cut(s) 704
FauNDI CATATG 1 cut(s) 944
FbaI TGATCA 1 cut(s) 1071
Fnu4HI GCNGC 3 cut(s) 47, 148, 917
FokI GGATG 6 cut(s) 44, 224, 292, 628, 674, 848
Fsp4HI GCNGC 3 cut(s) 47, 148, 917
FspBI CTAG 1 cut(s) 284
GlaI GCGC 2 cut(s) 246, 348
GluI GCNGC 3 cut(s) 47, 148, 917
GsaI CCCAGC 1 cut(s) 995
HaeII RGCGCY 1 cut(s) 350
HaeIII GGCC 3 cut(s) 336, 919, 949
HapII CCGG 3 cut(s) 427, 476, 637
HgaI GACGC 2 cut(s) 370, 714
HhaI GCGC 2 cut(s) 247, 349
Hin1I GRCGYC 2 cut(s) 362, 725
Hin1II CATG 5 cut(s) 133, 393, 1007, 1072, 1078
Hin6I GCGC 2 cut(s) 245, 347
HinP1I GCGC 2 cut(s) 245, 347
HincII GTYRAC 1 cut(s) 1080
HindII GTYRAC 1 cut(s) 1080
HinfI GANTC 3 cut(s) 436, 827, 1010
HpaI GTTAAC 1 cut(s) 1080
HpaII CCGG 3 cut(s) 427, 476, 637
HphI GGTGA 6 cut(s) 112, 445, 541, 730, 986, 1070
Hpy166II GTNNAC 3 cut(s) 451, 532, 1080
Hpy188I TCNGA 3 cut(s) 504, 835, 890
Hpy188III TCNNGA 4 cut(s) 67, 130, 342, 440
Hpy8I GTNNAC 3 cut(s) 451, 532, 1080
Hpy99I CGWCG 2 cut(s) 730, 835
HpyAV CCTTC 5 cut(s) 266, 348, 578, 1042, 1055
HpyCH4III ACNGT 5 cut(s) 78, 95, 518, 768, 956
HpyCH4IV ACGT 3 cut(s) 487, 565, 771
HpyCH4V TGCA 7 cut(s) 46, 323, 558, 654, 706, 859, 970
HpyF10VI GCNNNNNNNGC 2 cut(s) 144, 731
HpyF3I CTNAG 4 cut(s) 575, 783, 852, 933
HpySE526I ACGT 3 cut(s) 487, 565, 771
Hsp92I GRCGYC 2 cut(s) 362, 725
Hsp92II CATG 5 cut(s) 133, 393, 1007, 1072, 1078
HspAI GCGC 2 cut(s) 245, 347
Ksp22I TGATCA 1 cut(s) 1071
KspAI GTTAAC 1 cut(s) 1080
Kzo9I GATC 4 cut(s) 17, 102, 667, 1071
LmnI GCTCC 1 cut(s) 344
Lsp1109I GCAGC 1 cut(s) 58
LweI GCATC 5 cut(s) 14, 134, 270, 650, 838
MaeI CTAG 1 cut(s) 284
MaeII ACGT 3 cut(s) 487, 565, 771
MaeIII GTNAC 1 cut(s) 974
MalI GATC 4 cut(s) 19, 104, 669, 1073
MboI GATC 4 cut(s) 17, 102, 667, 1071
MboII GAAGA 5 cut(s) 134, 394, 800, 851, 992
MfeI CAATTG 1 cut(s) 375
MhlI GDGCHC 1 cut(s) 179
MluCI AATT 6 cut(s) 125, 159, 227, 248, 375, 461
MlyI GAGTC 1 cut(s) 1019
MmeI TCCRAC 2 cut(s) 482, 813
MseI TTAA 3 cut(s) 293, 1032, 1079
MslI CAYNNNNRTG 2 cut(s) 646, 1073
MspA1I CMGCKG 1 cut(s) 916
MspI CCGG 3 cut(s) 427, 476, 637
MspR9I CCNGG 7 cut(s) 62, 427, 458, 476, 477, 638, 984
MunI CAATTG 1 cut(s) 375
Mva1269I GAATGC 1 cut(s) 48
MvaI CCWGG 3 cut(s) 62, 458, 984
MwoI GCNNNNNNNGC 2 cut(s) 144, 731
NciI CCSGG 4 cut(s) 427, 476, 477, 638
NdeI CATATG 1 cut(s) 944
NdeII GATC 4 cut(s) 17, 102, 667, 1071
NlaIII CATG 5 cut(s) 133, 393, 1007, 1072, 1078
NlaIV GGNNCC 1 cut(s) 176
NmuCI GTSAC 1 cut(s) 974
NspI RCATGY 1 cut(s) 393
NspV TTCGAA 1 cut(s) 34
PagI TCATGA 1 cut(s) 129
PciI ACATGT 1 cut(s) 389
PcsI WCGNNNNNNNCGW 1 cut(s) 177
PctI GAATGC 1 cut(s) 48
PfeI GAWTC 2 cut(s) 436, 827
PflFI GACNNNGTC 1 cut(s) 76
PflMI CCANNNNNTGG 1 cut(s) 463
PkrI GCNGC 3 cut(s) 48, 149, 918
PleI GAGTC 1 cut(s) 1018
PpsI GAGTC 1 cut(s) 1018
Ppu21I YACGTR 2 cut(s) 488, 566
PscI ACATGT 1 cut(s) 389
Psp6I CCWGG 3 cut(s) 60, 456, 982
PspFI CCCAGC 1 cut(s) 991
PspGI CCWGG 3 cut(s) 60, 456, 982
PspN4I GGNNCC 1 cut(s) 176
PspPI GGNCC 1 cut(s) 335
PsyI GACNNNGTC 1 cut(s) 76
RsaI GTAC 3 cut(s) 183, 299, 770
RsaNI GTAC 3 cut(s) 182, 298, 769
RseI CAYNNNNRTG 2 cut(s) 646, 1073
SaqAI TTAA 3 cut(s) 293, 1032, 1079
SatI GCNGC 3 cut(s) 47, 148, 917
Sau3AI GATC 4 cut(s) 17, 102, 667, 1071
Sau96I GGNCC 1 cut(s) 335
SchI GAGTC 1 cut(s) 1019
ScrFI CCNGG 7 cut(s) 62, 427, 458, 476, 477, 638, 984
SduI GDGCHC 1 cut(s) 179
SfaNI GCATC 5 cut(s) 14, 134, 270, 650, 838
SfuI TTCGAA 1 cut(s) 34
SmaI CCCGGG 1 cut(s) 477
SmiMI CAYNNNNRTG 2 cut(s) 646, 1073
Sse9I AATT 6 cut(s) 125, 159, 227, 248, 375, 461
SsiI CCGC 4 cut(s) 148, 711, 908, 916
SspMI CTAG 1 cut(s) 284
StyD4I CCNGG 7 cut(s) 60, 425, 456, 474, 475, 636, 982
TaaI ACNGT 5 cut(s) 78, 95, 518, 768, 956
TaiI ACGT 3 cut(s) 490, 568, 774
TaqI TCGA 2 cut(s) 34, 728
TaqII GACCGA 1 cut(s) 284
TasI AATT 6 cut(s) 125, 159, 227, 248, 375, 461
TauI GCSGC 2 cut(s) 150, 919
TfiI GAWTC 2 cut(s) 436, 827
Tru1I TTAA 3 cut(s) 293, 1032, 1079
Tru9I TTAA 3 cut(s) 293, 1032, 1079
TscAI CASTG 1 cut(s) 961
TseFI GTSAC 1 cut(s) 974
TseI GCWGC 1 cut(s) 46
Tsp45I GTSAC 1 cut(s) 974
TspDTI ATGAA 6 cut(s) 46, 118, 146, 554, 852, 992
TspGWI ACGGA 1 cut(s) 224
TspMI CCCGGG 1 cut(s) 475
TspRI CASTG 1 cut(s) 961
Tth111I GACNNNGTC 1 cut(s) 76
Van91I CCANNNNNTGG 1 cut(s) 463
XapI RAATTY 1 cut(s) 125
XceI RCATGY 1 cut(s) 393
XmaI CCCGGG 1 cut(s) 475
XspI CTAG 1 cut(s) 284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.