Rh3BG122100

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0002876
Physical Location & Seq
Reverse (-)
10026380 .. 10028777
2398 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG122100.1

Sequence Viewer

Length: 1185 bp
ATGGGAATGCAGCCGTGTTTCATCCAGGTCGTGAGGACACCGTCCAAGAACATAGACAGTTTGGTGATCCCTCCTCCATTTGTAGAAGAATTCATGAATGGCGACAGATGCCGCAAAGGGAAAATTATACTGGACGGGTGCCCCGTACTTCAATGGGTGGTTGAAATAAATACGGATGGTAATGGGGTGGAATTGGGCGATGGGTGGGCGCAATTTGTTGCCCAAAATATGACCGAAGGCTGGATGCTAGTGATGTTAAGGTACGAGGGGGCAATGATATTTTATGCAAGGAGGTATGGCCCTTCAGGAGCGCTGATGGTGGGGACGCCAAATGGGACAATTGTGAAGAAAACATGTGCTTCAGGTGGGGGCGTTTTACCATCATTTGCCCGGTTGGTGATTCCCGATGGAGTGAACAGCCAGGAATTGGACATACCCCCGGGGATATTACGTGGAACAGCAGGTCGGAACAGGATTGACAGTAAAATATGGGTGAACAATATGAACTGGGGAATGTCGTGCAACGCACGTATCCAGCTAAGCGAGGAAGGAGGAGTAATAAAAGGAGGTTGGGAAATGTTTGTGAGGGCAAACATTAGCCGGGCATCCAAGTGGTGCATTGTGGATGTAGATCGTTTCAAGCAGGAGGTAAGAGTGATGGCATTTGTGCAATGCGGGGGGGTGATGGCGTCGAGGGCAATGATGAGTGTCCAAACAATAACTGATAGACTGTACGTTGATAGTGCTGAGGAAGACTACCCTGACACAAATGACAATGATGAGGGAGATGATTCGTCGGATGAAGAACAAGATGCTGAGTTGCAACAAAATCCAATCCCAAGTGTTGTGGTCAGAGTGGGAGAGAAATATGCGGTAAGTATATTACAACATGAGTTATACTTATGTCTTCAAGCCATGACTACCGTTGATGACAAAAGGTATTACGGTGAGACGCAACTCCCCACGGGATATGCAAAGAAATATTTCAAGGGGGTCCGTAGTCGTCGGTGTGTTGTCAGGACTGGAGACGGACAATCATGGGATGTTTCCTTTCAGAAGTCTAATGACACCTATAGGTTCAGCGGCCGTTTCGTGGAACTGAGGACAGCATATGGCCTTACAGTGGGAAACAAATGCAAGGTGACTTACCTGGGAGGCTGGGTGTTCTTCATGGAGAGTCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

394

Amino Acids

44.0

Weight (kDa)

5.41

Isoelectric Point (pI)

38.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 8 - 90 4.4e-06 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 452
AccB1I GGYRCC 1 cut(s) 138
AccB7I CCANNNNNTGG 1 cut(s) 427
AciI CCGC 4 cut(s) 112, 675, 872, 1083
AclWI GGATC 1 cut(s) 61
AcoI YGGCCR 1 cut(s) 1084
AcsI RAATTY 1 cut(s) 89
AcuI CTGAAG 2 cut(s) 288, 345
AcyI GRCGYC 2 cut(s) 326, 689
AfaI GTAC 3 cut(s) 147, 263, 734
AfeI AGCGCT 1 cut(s) 312
AfiI CCNNNNNNNGG 4 cut(s) 240, 427, 1093, 1123
AflIII ACRYGT 1 cut(s) 353
AgsI TTSAA 5 cut(s) 152, 164, 640, 911, 988
AjnI CCWGG 3 cut(s) 24, 420, 1149
AluBI AGCT 1 cut(s) 538
AluI AGCT 1 cut(s) 538
Alw26I GTCTC 2 cut(s) 944, 1020
AlwI GGATC 1 cut(s) 61
Ama87I CYCGRG 1 cut(s) 439
Aor51HI AGCGCT 1 cut(s) 312
AoxI GGCC 3 cut(s) 298, 1084, 1114
ApeKI GCWGC 1 cut(s) 10
ApoI RAATTY 1 cut(s) 89
Asp700I GAANNNNTTC 1 cut(s) 983
AspLEI GCGC 2 cut(s) 211, 313
AspS9I GGNCC 2 cut(s) 299, 994
AsuC2I CCSGG 4 cut(s) 391, 440, 441, 602
AsuHPI GGTGA 6 cut(s) 76, 409, 505, 694, 959, 1153
AvaI CYCGRG 1 cut(s) 439
AvaII GGWCC 1 cut(s) 994
BaeGI GKGCMC 1 cut(s) 143
BaeI ACNNNNGTAYC 2 cut(s) 514, 547
BanI GGYRCC 1 cut(s) 138
BbsI GAAGAC 2 cut(s) 759, 899
BbvCI CCTCAGC 1 cut(s) 747
BbvI GCAGC 1 cut(s) 22
BccI CCATC 7 cut(s) 170, 194, 310, 388, 401, 652, 679
BceAI ACGGC 1 cut(s) 1071
BciT130I CCWGG 3 cut(s) 26, 422, 1151
BciVI GTATCC 1 cut(s) 542
BcnI CCSGG 4 cut(s) 391, 440, 441, 602
BcoDI GTCTC 2 cut(s) 944, 1020
BfaI CTAG 1 cut(s) 248
BfmI CTRYAG 1 cut(s) 1072
BfoI RGCGCY 1 cut(s) 314
BfuAI ACCTGC 1 cut(s) 452
BfuI GTATCC 1 cut(s) 542
BisI GCNGC 3 cut(s) 11, 112, 1084
BlpI GCTNAGC 1 cut(s) 539
BlsI GCNGC 3 cut(s) 12, 113, 1085
Bme1390I CCNGG 7 cut(s) 26, 391, 422, 440, 441, 602, 1151
Bme18I GGWCC 1 cut(s) 994
BmeT110I CYCGRG 1 cut(s) 439
BmgT120I GGNCC 2 cut(s) 299, 994
BmiI GGNNCC 2 cut(s) 140, 995
BmrFI CCNGG 7 cut(s) 26, 391, 422, 440, 441, 602, 1151
BmrI ACTGGG 1 cut(s) 517
BmsI GCATC 4 cut(s) 98, 234, 614, 802
BmuI ACTGGG 1 cut(s) 517
BpiI GAAGAC 2 cut(s) 759, 899
BpmI CTGGAG 1 cut(s) 1044
Bpu10I CCTNAGC 1 cut(s) 747
Bpu1102I GCTNAGC 1 cut(s) 539
BpuMI CCSGG 4 cut(s) 391, 440, 441, 602
BsaAI YACGTR 2 cut(s) 452, 530
BsaBI GATNNNNATC 1 cut(s) 630
BsaHI GRCGYC 2 cut(s) 326, 689
BsaJI CCNNGG 5 cut(s) 438, 439, 440, 963, 1150
BsaXI ACNNNNNCTCC 3 cut(s) 1146, 1166, 1176
Bsc4I CCNNNNNNNGG 4 cut(s) 240, 427, 1093, 1123
Bse1I ACTGG 3 cut(s) 135, 512, 1027
Bse3DI GCAATG 3 cut(s) 279, 677, 705
Bse8I GATNNNNATC 1 cut(s) 630
BseBI CCWGG 3 cut(s) 26, 422, 1151
BseDI CCNNGG 5 cut(s) 438, 439, 440, 963, 1150
BseGI GGATG 7 cut(s) 21, 181, 249, 605, 631, 805, 1048
BseJI GATNNNNATC 1 cut(s) 630
BseLI CCNNNNNNNGG 4 cut(s) 240, 427, 1093, 1123
BseMI GCAATG 3 cut(s) 279, 677, 705
BseMII CTCAG 3 cut(s) 738, 807, 1091
BseNI ACTGG 3 cut(s) 135, 512, 1027
BseRI GAGGAG 2 cut(s) 63, 567
BseSI GKGCMC 1 cut(s) 143
BseX3I CGGCCG 1 cut(s) 1084
BseXI GCAGC 1 cut(s) 22
BseYI CCCAGC 1 cut(s) 1158
Bsh1285I CGRYCG 1 cut(s) 1087
BshFI GGCC 3 cut(s) 300, 1086, 1116
BshNI GGYRCC 1 cut(s) 138
BsiEI CGRYCG 1 cut(s) 1087
BsiHKCI CYCGRG 1 cut(s) 439
BsiSI CCGG 3 cut(s) 391, 440, 601
BslFI GGGAC 2 cut(s) 337, 349
BslI CCNNNNNNNGG 4 cut(s) 240, 427, 1093, 1123
BsmAI GTCTC 2 cut(s) 944, 1020
BsmBI CGTCTC 2 cut(s) 944, 1020
BsmFI GGGAC 2 cut(s) 337, 349
BsmI GAATGC 1 cut(s) 12
BsnI GGCC 3 cut(s) 300, 1086, 1116
BsoBI CYCGRG 1 cut(s) 439
Bsp1286I GDGCHC 1 cut(s) 143
Bsp143I GATC 2 cut(s) 66, 631
Bsp1720I GCTNAGC 1 cut(s) 539
BspACI CCGC 4 cut(s) 112, 675, 872, 1083
BspANI GGCC 3 cut(s) 300, 1086, 1116
BspCNI CTCAG 3 cut(s) 739, 808, 1092
BspHI TCATGA 1 cut(s) 93
BspLI GGNNCC 2 cut(s) 140, 995
BspMI ACCTGC 1 cut(s) 452
BspPI GGATC 1 cut(s) 61
BspT107I GGYRCC 1 cut(s) 138
BsrDI GCAATG 3 cut(s) 279, 677, 705
BsrI ACTGG 3 cut(s) 135, 512, 1027
BssECI CCNNGG 5 cut(s) 438, 439, 440, 963, 1150
BssMI GATC 2 cut(s) 66, 631
BssNI GRCGYC 2 cut(s) 326, 689
Bst2UI CCWGG 3 cut(s) 26, 422, 1151
Bst4CI ACNGT 7 cut(s) 42, 59, 482, 732, 925, 947, 1123
BstACI GRCGYC 2 cut(s) 326, 689
BstBAI YACGTR 2 cut(s) 452, 530
BstDEI CTNAG 4 cut(s) 539, 747, 816, 1100
BstDSI CCRYGG 1 cut(s) 963
BstF5I GGATG 7 cut(s) 21, 181, 249, 605, 631, 805, 1048
BstH2I RGCGCY 1 cut(s) 314
BstHHI GCGC 2 cut(s) 211, 313
BstKTI GATC 2 cut(s) 69, 634
BstMAI GTCTC 2 cut(s) 944, 1020
BstMBI GATC 2 cut(s) 66, 631
BstMCI CGRYCG 1 cut(s) 1087
BstMWI GCNNNNNNNGC 2 cut(s) 108, 695
BstNI CCWGG 3 cut(s) 26, 422, 1151
BstNSI RCATGY 1 cut(s) 357
BstSCI CCNGG 7 cut(s) 24, 389, 420, 438, 439, 600, 1149
BstSFI CTRYAG 1 cut(s) 1072
BstSLI GKGCMC 1 cut(s) 143
BstV1I GCAGC 1 cut(s) 22
BstV2I GAAGAC 2 cut(s) 759, 899
BstZI CGGCCG 1 cut(s) 1084
BsuI GTATCC 1 cut(s) 542
BsuRI GGCC 3 cut(s) 300, 1086, 1116
BtgI CCRYGG 1 cut(s) 963
BtgZI GCGATG 1 cut(s) 213
BtsCI GGATG 7 cut(s) 21, 181, 249, 605, 631, 805, 1048
BtsIMutI CAGTG 1 cut(s) 1128
BveI ACCTGC 1 cut(s) 452
CciI TCATGA 1 cut(s) 93
CfoI GCGC 2 cut(s) 211, 313
Cfr13I GGNCC 2 cut(s) 299, 994
Cfr9I CCCGGG 1 cut(s) 439
CseI GACGC 3 cut(s) 334, 678, 961
Csp6I GTAC 3 cut(s) 146, 262, 733
CspCI CAANNNNNGTGG 2 cut(s) 828, 863
CviAII CATG 6 cut(s) 94, 354, 890, 916, 1038, 1171
CviQI GTAC 3 cut(s) 146, 262, 733
DdeI CTNAG 4 cut(s) 539, 747, 816, 1100
DpnI GATC 2 cut(s) 68, 633
DpnII GATC 2 cut(s) 66, 631
EaeI YGGCCR 1 cut(s) 1084
EagI CGGCCG 1 cut(s) 1084
EclXI CGGCCG 1 cut(s) 1084
Eco47I GGWCC 1 cut(s) 994
Eco47III AGCGCT 1 cut(s) 312
Eco52I CGGCCG 1 cut(s) 1084
Eco57I CTGAAG 2 cut(s) 288, 345
Eco88I CYCGRG 1 cut(s) 439
EcoRI GAATTC 1 cut(s) 89
EcoRII CCWGG 3 cut(s) 24, 420, 1149
Esp3I CGTCTC 2 cut(s) 944, 1020
FaeI CATG 6 cut(s) 97, 357, 893, 919, 1041, 1174
FaqI GGGAC 2 cut(s) 337, 349
FatI CATG 6 cut(s) 93, 353, 889, 915, 1037, 1170
FauI CCCGC 1 cut(s) 668
FauNDI CATATG 1 cut(s) 1111
Fnu4HI GCNGC 3 cut(s) 11, 112, 1084
FokI GGATG 7 cut(s) 8, 188, 256, 592, 638, 812, 1055
Fsp4HI GCNGC 3 cut(s) 11, 112, 1084
FspBI CTAG 1 cut(s) 248
GlaI GCGC 2 cut(s) 210, 312
GluI GCNGC 3 cut(s) 11, 112, 1084
GsaI CCCAGC 1 cut(s) 1162
GsuI CTGGAG 1 cut(s) 1044
HaeII RGCGCY 1 cut(s) 314
HaeIII GGCC 3 cut(s) 300, 1086, 1116
HapII CCGG 3 cut(s) 391, 440, 601
HgaI GACGC 3 cut(s) 334, 678, 961
HhaI GCGC 2 cut(s) 211, 313
Hin1I GRCGYC 2 cut(s) 326, 689
Hin1II CATG 6 cut(s) 97, 357, 893, 919, 1041, 1174
Hin6I GCGC 2 cut(s) 209, 311
HinP1I GCGC 2 cut(s) 209, 311
HinfI GANTC 3 cut(s) 400, 791, 1177
HpaII CCGG 3 cut(s) 391, 440, 601
HphI GGTGA 6 cut(s) 76, 409, 505, 694, 959, 1153
Hpy166II GTNNAC 2 cut(s) 415, 496
Hpy188I TCNGA 4 cut(s) 468, 799, 854, 1056
Hpy188III TCNNGA 5 cut(s) 31, 94, 306, 404, 1018
Hpy8I GTNNAC 2 cut(s) 415, 496
Hpy99I CGWCG 3 cut(s) 694, 799, 1008
HpyAV CCTTC 3 cut(s) 230, 312, 542
HpyCH4III ACNGT 7 cut(s) 42, 59, 482, 732, 925, 947, 1123
HpyCH4IV ACGT 3 cut(s) 451, 529, 735
HpyCH4V TGCA 8 cut(s) 10, 287, 522, 618, 670, 823, 974, 1137
HpyF10VI GCNNNNNNNGC 2 cut(s) 108, 695
HpyF3I CTNAG 4 cut(s) 539, 747, 816, 1100
HpySE526I ACGT 3 cut(s) 451, 529, 735
Hsp92I GRCGYC 2 cut(s) 326, 689
Hsp92II CATG 6 cut(s) 97, 357, 893, 919, 1041, 1174
HspAI GCGC 2 cut(s) 209, 311
Kzo9I GATC 2 cut(s) 66, 631
LmnI GCTCC 1 cut(s) 308
Lsp1109I GCAGC 1 cut(s) 22
LweI GCATC 4 cut(s) 98, 234, 614, 802
MaeI CTAG 1 cut(s) 248
MaeII ACGT 3 cut(s) 451, 529, 735
MaeIII GTNAC 1 cut(s) 1141
MalI GATC 2 cut(s) 68, 633
MboI GATC 2 cut(s) 66, 631
MboII GAAGA 6 cut(s) 98, 358, 764, 815, 899, 1159
MfeI CAATTG 1 cut(s) 339
MhlI GDGCHC 1 cut(s) 143
MluCI AATT 6 cut(s) 89, 123, 191, 212, 339, 425
MmeI TCCRAC 2 cut(s) 446, 777
MroXI GAANNNNTTC 1 cut(s) 983
MseI TTAA 1 cut(s) 257
MslI CAYNNNNRTG 1 cut(s) 610
MspA1I CMGCKG 1 cut(s) 1083
MspI CCGG 3 cut(s) 391, 440, 601
MspR9I CCNGG 7 cut(s) 26, 391, 422, 440, 441, 602, 1151
MunI CAATTG 1 cut(s) 339
Mva1269I GAATGC 1 cut(s) 12
MvaI CCWGG 3 cut(s) 26, 422, 1151
MwoI GCNNNNNNNGC 2 cut(s) 108, 695
NciI CCSGG 4 cut(s) 391, 440, 441, 602
NdeI CATATG 1 cut(s) 1111
NdeII GATC 2 cut(s) 66, 631
NlaIII CATG 6 cut(s) 97, 357, 893, 919, 1041, 1174
NlaIV GGNNCC 2 cut(s) 140, 995
NmuCI GTSAC 1 cut(s) 1141
NspI RCATGY 1 cut(s) 357
PagI TCATGA 1 cut(s) 93
PciI ACATGT 1 cut(s) 353
PcsI WCGNNNNNNNCGW 1 cut(s) 141
PctI GAATGC 1 cut(s) 12
PdmI GAANNNNTTC 1 cut(s) 983
PfeI GAWTC 2 cut(s) 400, 791
PflFI GACNNNGTC 1 cut(s) 40
PflMI CCANNNNNTGG 1 cut(s) 427
PkrI GCNGC 3 cut(s) 12, 113, 1085
Ppu21I YACGTR 2 cut(s) 452, 530
PscI ACATGT 1 cut(s) 353
Psp6I CCWGG 3 cut(s) 24, 420, 1149
PspFI CCCAGC 1 cut(s) 1158
PspGI CCWGG 3 cut(s) 24, 420, 1149
PspN4I GGNNCC 2 cut(s) 140, 995
PspPI GGNCC 2 cut(s) 299, 994
PsyI GACNNNGTC 1 cut(s) 40
RsaI GTAC 3 cut(s) 147, 263, 734
RsaNI GTAC 3 cut(s) 146, 262, 733
RseI CAYNNNNRTG 1 cut(s) 610
SaqAI TTAA 1 cut(s) 257
SatI GCNGC 3 cut(s) 11, 112, 1084
Sau3AI GATC 2 cut(s) 66, 631
Sau96I GGNCC 2 cut(s) 299, 994
ScrFI CCNGG 7 cut(s) 26, 391, 422, 440, 441, 602, 1151
SduI GDGCHC 1 cut(s) 143
SfaNI GCATC 4 cut(s) 98, 234, 614, 802
SfcI CTRYAG 1 cut(s) 1072
SinI GGWCC 1 cut(s) 994
SmaI CCCGGG 1 cut(s) 441
SmiMI CAYNNNNRTG 1 cut(s) 610
Sse9I AATT 6 cut(s) 89, 123, 191, 212, 339, 425
SsiI CCGC 4 cut(s) 112, 675, 872, 1083
SspI AATATT 1 cut(s) 983
SspMI CTAG 1 cut(s) 248
StyD4I CCNGG 7 cut(s) 24, 389, 420, 438, 439, 600, 1149
TaaI ACNGT 7 cut(s) 42, 59, 482, 732, 925, 947, 1123
TaiI ACGT 3 cut(s) 454, 532, 738
TaqI TCGA 1 cut(s) 692
TaqII GACCGA 1 cut(s) 248
TasI AATT 6 cut(s) 89, 123, 191, 212, 339, 425
TauI GCSGC 2 cut(s) 114, 1086
TfiI GAWTC 2 cut(s) 400, 791
Tru1I TTAA 1 cut(s) 257
Tru9I TTAA 1 cut(s) 257
TscAI CASTG 1 cut(s) 1128
TseFI GTSAC 1 cut(s) 1141
TseI GCWGC 1 cut(s) 10
Tsp45I GTSAC 1 cut(s) 1141
TspDTI ATGAA 6 cut(s) 10, 82, 110, 518, 816, 1159
TspGWI ACGGA 3 cut(s) 188, 986, 1044
TspMI CCCGGG 1 cut(s) 439
TspRI CASTG 1 cut(s) 1128
Tth111I GACNNNGTC 1 cut(s) 40
Van91I CCANNNNNTGG 1 cut(s) 427
VpaK11BI GGWCC 1 cut(s) 994
XapI RAATTY 1 cut(s) 89
XceI RCATGY 1 cut(s) 357
XmaI CCCGGG 1 cut(s) 439
XmnI GAANNNNTTC 1 cut(s) 983
XspI CTAG 1 cut(s) 248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.