Rh1AG005200

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0002876
Physical Location & Seq
Forward (+)
706200 .. 708292
2093 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG005200.1

Sequence Viewer

Length: 1137 bp
ATGGCTTCAGGAAGTGGATCACACCGTCCAATGCAGTTGGGAAGGCAGCTGTGTTTCATCCAAGTTGTGAGGAGCCCGTCAAAGAATACGGAGAGTTTGGTCATCCCTCCTCCATTTGTGATAGAGTTCATGAATGGTGACAGATGCCGGCAGGGAAGGATAAAACTAGAAGGATTGGAACAGTGCCAGTGGCGGGTAAAAATAAATACAGATGGGAGGGGAGTTGAATTGGGTGAAGGGTGGGCGGAATTTGTGGGGGAAAATTTGGAGGACGGGTGGATGCTAGTGCTATTAAGGTACGAGGGGGCAATGACTTTTTCTGCCAGGAGATATGGCCGGTCAGGAGCTCTGATGGTGACAGCACCAAGGGGGAGACAGACAAAGGGGACACCACGTGGAGTTGGGGGCATTTATCCATCATTTGCACAGTTGGTTATTCCAAGTGGAATGAACAGCGAAGTGCTGGAAATACCAGCAGGGATCTTTTGTGGAGGATCAAGTCGAACCACGATTGAAAGTAAGGTTTGGTTGAATAATATGAATTGGGGGATGTCGTGCAATGCACGTATCAAGGTAAGTGAATATAGAGGATTCATAAAAGGAGGTTGGGACAGGTGGGTGAGTGCAAACATTGTAAGAGTATGCAAGTGGTGCATTGTGGATTATAATCGTTCAAAAAATGAGGCAACTGTGATGGCGTTTCTGCAATGTGGAGGGGTGATGTCGAACGTGGCAATGATGAATGCCATAACAATCGGTGATGCTATGTACATTGATAGTTGTGATGAAGAATATTCGGAGGATTCAAAGGAAGATGAGGAAGATCTGACTGATAAAGGAGAAGCTGGTCATATGGTACAGTTTCCAATACCTCAAAGTGTGATCCGAGTTGGAAAAAAATATGGGCAACTTTCCACAAAGTATGCAAAGCAATATTTCCCTGGTGTCGGTAGTCGTCAGGTTGTAGTGAGGACTGGAGATGGACAATCATGGGAGGTTCCATTTTTGAAGTCAAATCAAACATATAGGTACAGCAGAGGCTTTGTGGAACTAAGGAAAGGATATGGGCTGAAGGTGGGAAACAAATGCAAGGTTTCGTGCCAAGGAAACTGGGTTTTTGTGATGGAGAAGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0007275 GO:0008150 GO:0009553 GO:0009566 GO:0009567 GO:0019953 GO:0022414 GO:0032501 GO:0032502 GO:0044703 GO:0048229 GO:0048856 GO:0051704
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

378

Amino Acids

42.25

Weight (kDa)

9.03

Isoelectric Point (pI)

40.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 19 - 106 3.9e-10 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 666
AciI CCGC 2 cut(s) 193, 245
AclWI GGATC 4 cut(s) 25, 488, 502, 877
AcoI YGGCCR 1 cut(s) 334
AcsI RAATTY 2 cut(s) 248, 262
AcuI CTGAAG 1 cut(s) 1091
AcvI CACGTG 1 cut(s) 395
AdeI CACNNNGTG 1 cut(s) 395
AfaI GTAC 4 cut(s) 299, 770, 858, 1031
AfiI CCNNNNNNNGG 2 cut(s) 193, 947
AgsI TTSAA 6 cut(s) 227, 515, 532, 675, 807, 1009
AjnI CCWGG 2 cut(s) 323, 940
AluBI AGCT 3 cut(s) 49, 347, 845
AluI AGCT 3 cut(s) 49, 347, 845
Alw21I GWGCWC 1 cut(s) 349
Alw26I GTCTC 1 cut(s) 367
AlwI GGATC 4 cut(s) 25, 488, 502, 877
AoxI GGCC 1 cut(s) 334
ApeKI GCWGC 1 cut(s) 46
ApoI RAATTY 2 cut(s) 248, 262
AsuHPI GGTGA 6 cut(s) 149, 245, 367, 631, 730, 770
BanII GRGCYC 2 cut(s) 77, 349
BbrPI CACGTG 1 cut(s) 395
Bbv12I GWGCWC 1 cut(s) 349
BbvI GCAGC 1 cut(s) 58
BccI CCATC 6 cut(s) 206, 346, 424, 688, 974, 1117
BciT130I CCWGG 2 cut(s) 325, 942
BcoDI GTCTC 1 cut(s) 367
BfaI CTAG 2 cut(s) 167, 284
BglII AGATCT 1 cut(s) 823
BisI GCNGC 1 cut(s) 47
BlsI GCNGC 1 cut(s) 48
Bme1390I CCNGG 2 cut(s) 325, 942
BmiI GGNNCC 2 cut(s) 74, 999
BmrFI CCNGG 2 cut(s) 325, 942
BmrI ACTGGG 1 cut(s) 1120
BmsI GCATC 3 cut(s) 134, 270, 751
BmuI ACTGGG 1 cut(s) 1120
BpmI CTGGAG 1 cut(s) 996
BsaAI YACGTR 2 cut(s) 395, 566
BsaBI GATNNNNATC 1 cut(s) 666
BsaJI CCNNGG 3 cut(s) 365, 940, 1102
BsaXI ACNNNNNCTCC 3 cut(s) 260, 290, 1118
Bsc4I CCNNNNNNNGG 2 cut(s) 193, 947
Bse118I RCCGGY 2 cut(s) 147, 336
Bse1I ACTGG 3 cut(s) 187, 979, 1115
Bse3DI GCAATG 4 cut(s) 315, 565, 713, 741
Bse8I GATNNNNATC 1 cut(s) 666
BseBI CCWGG 2 cut(s) 325, 942
BseDI CCNNGG 3 cut(s) 365, 940, 1102
BseGI GGATG 4 cut(s) 57, 102, 285, 555
BseJI GATNNNNATC 1 cut(s) 666
BseLI CCNNNNNNNGG 2 cut(s) 193, 947
BseMI GCAATG 4 cut(s) 315, 565, 713, 741
BseNI ACTGG 3 cut(s) 187, 979, 1115
BseRI GAGGAG 2 cut(s) 85, 99
BseXI GCAGC 1 cut(s) 58
BshFI GGCC 1 cut(s) 336
BsiHKAI GWGCWC 1 cut(s) 349
BsiSI CCGG 2 cut(s) 148, 337
BslFI GGGAC 2 cut(s) 400, 623
BslI CCNNNNNNNGG 2 cut(s) 193, 947
BsmAI GTCTC 1 cut(s) 367
BsmFI GGGAC 2 cut(s) 400, 623
BsmI GAATGC 1 cut(s) 748
BsnI GGCC 1 cut(s) 336
Bsp1286I GDGCHC 2 cut(s) 77, 349
Bsp1407I TGTACA 1 cut(s) 768
Bsp143I GATC 5 cut(s) 17, 480, 494, 823, 882
BspACI CCGC 2 cut(s) 193, 245
BspANI GGCC 1 cut(s) 336
BspHI TCATGA 1 cut(s) 129
BspLI GGNNCC 2 cut(s) 74, 999
BspPI GGATC 4 cut(s) 25, 488, 502, 877
BsrDI GCAATG 4 cut(s) 315, 565, 713, 741
BsrFI RCCGGY 2 cut(s) 147, 336
BsrGI TGTACA 1 cut(s) 768
BsrI ACTGG 3 cut(s) 187, 979, 1115
BssAI RCCGGY 2 cut(s) 147, 336
BssECI CCNNGG 3 cut(s) 365, 940, 1102
BssMI GATC 5 cut(s) 17, 480, 494, 823, 882
BssT1I CCWWGG 2 cut(s) 365, 1102
Bst2UI CCWGG 2 cut(s) 325, 942
Bst4CI ACNGT 5 cut(s) 26, 183, 429, 691, 861
BstAPI GCANNNNNTGC 1 cut(s) 651
BstAUI TGTACA 1 cut(s) 768
BstBAI YACGTR 2 cut(s) 395, 566
BstC8I GCNNGC 1 cut(s) 149
BstDEI CTNAG 1 cut(s) 1052
BstF5I GGATG 4 cut(s) 57, 102, 285, 555
BstKTI GATC 5 cut(s) 20, 483, 497, 826, 885
BstMAI GTCTC 1 cut(s) 367
BstMBI GATC 5 cut(s) 17, 480, 494, 823, 882
BstMWI GCNNNNNNNGC 1 cut(s) 651
BstNI CCWGG 2 cut(s) 325, 942
BstSCI CCNGG 2 cut(s) 323, 940
BstV1I GCAGC 1 cut(s) 58
BstX2I RGATCY 2 cut(s) 480, 823
BstYI RGATCY 2 cut(s) 480, 823
BsuRI GGCC 1 cut(s) 336
BtsCI GGATG 4 cut(s) 57, 102, 285, 555
BtsIMutI CAGTG 2 cut(s) 188, 194
Cac8I GCNNGC 1 cut(s) 149
CciI TCATGA 1 cut(s) 129
Cfr10I RCCGGY 2 cut(s) 147, 336
Csp6I GTAC 4 cut(s) 298, 769, 857, 1030
CviAII CATG 2 cut(s) 130, 990
CviJI RGCY 8 cut(s) 5, 49, 75, 336, 347, 845, 1041, 1069
CviKI_1 RGCY 8 cut(s) 5, 49, 75, 336, 347, 845, 1041, 1069
CviQI GTAC 4 cut(s) 298, 769, 857, 1030
DdeI CTNAG 1 cut(s) 1052
DpnI GATC 5 cut(s) 19, 482, 496, 825, 884
DpnII GATC 5 cut(s) 17, 480, 494, 823, 882
DraIII CACNNNGTG 1 cut(s) 395
EaeI YGGCCR 1 cut(s) 334
EciI GGCGGA 1 cut(s) 260
Ecl136II GAGCTC 1 cut(s) 347
Eco130I CCWWGG 2 cut(s) 365, 1102
Eco24I GRGCYC 2 cut(s) 77, 349
Eco53kI GAGCTC 1 cut(s) 347
Eco57I CTGAAG 1 cut(s) 1091
Eco72I CACGTG 1 cut(s) 395
EcoICRI GAGCTC 1 cut(s) 347
EcoRII CCWGG 2 cut(s) 323, 940
EcoT14I CCWWGG 2 cut(s) 365, 1102
EcoT38I GRGCYC 2 cut(s) 77, 349
ErhI CCWWGG 2 cut(s) 365, 1102
FaeI CATG 2 cut(s) 133, 993
FaqI GGGAC 2 cut(s) 400, 623
FatI CATG 2 cut(s) 129, 989
FauI CCCGC 1 cut(s) 186
FauNDI CATATG 1 cut(s) 852
Fnu4HI GCNGC 1 cut(s) 47
FokI GGATG 4 cut(s) 44, 89, 292, 562
FriOI GRGCYC 2 cut(s) 77, 349
Fsp4HI GCNGC 1 cut(s) 47
FspBI CTAG 2 cut(s) 167, 284
GluI GCNGC 1 cut(s) 47
GsuI CTGGAG 1 cut(s) 996
HaeIII GGCC 1 cut(s) 336
HapII CCGG 2 cut(s) 148, 337
Hin1II CATG 2 cut(s) 133, 993
HinfI GANTC 2 cut(s) 591, 803
HpaII CCGG 2 cut(s) 148, 337
HphI GGTGA 6 cut(s) 149, 245, 367, 631, 730, 770
Hpy188I TCNGA 4 cut(s) 351, 799, 828, 887
Hpy188III TCNNGA 3 cut(s) 9, 130, 342
HpyAV CCTTC 5 cut(s) 36, 150, 164, 230, 1066
HpyCH4III ACNGT 5 cut(s) 26, 183, 429, 691, 861
HpyCH4IV ACGT 3 cut(s) 394, 565, 729
HpyF10VI GCNNNNNNNGC 1 cut(s) 651
HpyF3I CTNAG 1 cut(s) 1052
HpySE526I ACGT 3 cut(s) 394, 565, 729
Hsp92II CATG 2 cut(s) 133, 993
KroI GCCGGC 1 cut(s) 147
KroNI GCCGGC 1 cut(s) 149
Kzo9I GATC 5 cut(s) 17, 480, 494, 823, 882
LmnI GCTCC 2 cut(s) 72, 344
Lsp1109I GCAGC 1 cut(s) 58
LweI GCATC 3 cut(s) 134, 270, 751
MaeI CTAG 2 cut(s) 167, 284
MaeII ACGT 3 cut(s) 394, 565, 729
MaeIII GTNAC 2 cut(s) 137, 355
MalI GATC 5 cut(s) 19, 482, 496, 825, 884
MboI GATC 5 cut(s) 17, 480, 494, 823, 882
MboII GAAGA 3 cut(s) 800, 824, 833
MflI RGATCY 2 cut(s) 480, 823
MhlI GDGCHC 2 cut(s) 77, 349
MluCI AATT 4 cut(s) 227, 248, 262, 541
MmeI TCCRAC 1 cut(s) 871
MroNI GCCGGC 1 cut(s) 147
MseI TTAA 1 cut(s) 293
MspA1I CMGCKG 1 cut(s) 49
MspI CCGG 2 cut(s) 148, 337
MspR9I CCNGG 2 cut(s) 325, 942
Mva1269I GAATGC 1 cut(s) 748
MvaI CCWGG 2 cut(s) 325, 942
MwoI GCNNNNNNNGC 1 cut(s) 651
NaeI GCCGGC 1 cut(s) 149
NdeI CATATG 1 cut(s) 852
NdeII GATC 5 cut(s) 17, 480, 494, 823, 882
NgoMIV GCCGGC 1 cut(s) 147
NlaIII CATG 2 cut(s) 133, 993
NlaIV GGNNCC 2 cut(s) 74, 999
NmuCI GTSAC 2 cut(s) 137, 355
PagI TCATGA 1 cut(s) 129
PctI GAATGC 1 cut(s) 748
PdiI GCCGGC 1 cut(s) 149
PfeI GAWTC 2 cut(s) 591, 803
PkrI GCNGC 1 cut(s) 48
PmaCI CACGTG 1 cut(s) 395
PmlI CACGTG 1 cut(s) 395
Ppu21I YACGTR 2 cut(s) 395, 566
PsiI TTATAA 1 cut(s) 666
Psp124BI GAGCTC 1 cut(s) 349
Psp6I CCWGG 2 cut(s) 323, 940
PspCI CACGTG 1 cut(s) 395
PspGI CCWGG 2 cut(s) 323, 940
PspN4I GGNNCC 2 cut(s) 74, 999
PsuI RGATCY 2 cut(s) 480, 823
PvuII CAGCTG 1 cut(s) 49
RsaI GTAC 4 cut(s) 299, 770, 858, 1031
RsaNI GTAC 4 cut(s) 298, 769, 857, 1030
SacI GAGCTC 1 cut(s) 349
SaqAI TTAA 1 cut(s) 293
SatI GCNGC 1 cut(s) 47
Sau3AI GATC 5 cut(s) 17, 480, 494, 823, 882
ScrFI CCNGG 2 cut(s) 325, 942
SduI GDGCHC 2 cut(s) 77, 349
SfaNI GCATC 3 cut(s) 134, 270, 751
Sse9I AATT 4 cut(s) 227, 248, 262, 541
SsiI CCGC 2 cut(s) 193, 245
SspI AATATT 2 cut(s) 794, 935
SspMI CTAG 2 cut(s) 167, 284
SstI GAGCTC 1 cut(s) 349
StyD4I CCNGG 2 cut(s) 323, 940
StyI CCWWGG 2 cut(s) 365, 1102
TaaI ACNGT 5 cut(s) 26, 183, 429, 691, 861
TaiI ACGT 3 cut(s) 397, 568, 732
TaqI TCGA 2 cut(s) 502, 725
TasI AATT 4 cut(s) 227, 248, 262, 541
TatI WGTACW 1 cut(s) 768
TfiI GAWTC 2 cut(s) 591, 803
Tru1I TTAA 1 cut(s) 293
Tru9I TTAA 1 cut(s) 293
TscAI CASTG 2 cut(s) 188, 194
TseFI GTSAC 2 cut(s) 137, 355
TseI GCWGC 1 cut(s) 46
Tsp45I GTSAC 2 cut(s) 137, 355
TspDTI ATGAA 8 cut(s) 46, 118, 146, 464, 554, 583, 755, 801
TspGWI ACGGA 1 cut(s) 104
TspRI CASTG 2 cut(s) 188, 194
XapI RAATTY 2 cut(s) 248, 262
XspI CTAG 2 cut(s) 167, 284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.