Rh5AG305400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0002876
Physical Location & Seq
Forward (+)
43546689 .. 43548083
1395 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG305400.1

Sequence Viewer

Length: 828 bp
ATGACTTTTTCTGCCAGGAGATATGGCCGGTCAGGAGCTCTGATGGTGACGGCACCAAGGGGGAGACAGACAGAGGAGACACGACGTGGAGTTGGGGGCATTTATCCATCATTTGCACAGTTGGTTATTCCAGGTGGAATGAACAGCGAAGTACTGGAAATACCAGCAGGGATCTTCCGTGGAGGGTCAAGTCGAACCACGATTGAGAGCAAGGTTTGGTTGAATAATATGAATTGGGGGATGTCGTGCAATGCACGTATCAAGGTAAGTGAGGATGGAGGAGTCATAAAAGGAGGTTGGGATAGGTGGGTGAGTGCAAACATTGTAAGGGTATGCAAGTGGTGCATTGTGGATTATAATCGTTCAAAAAATGAGGCAACTGTGATGGCGTTTCTGCAATGTGGAGGGGTGATGTCGAATGTGCCAATGATGAATGCCGTAACAATCGGTGATGCTATGTACGTTGATAGTTGTCATGAAGAATATCCAGAGGATTCAGAGGAAGAGGAGGAAGATCAGCCTGATGGAGGAGAAGCTGGTCATATGGTACAGTTTCCAATACCTCAAACTGCGATCCGAGTTGGCAAAAATTATGGGCAACTTCCCACGGAGTATGCAAAACAATATTTCCCGGGTGTCGGTAGTCGTCAGGTTGTTGTGAGGACTGGAGATGGACAATCATGGGAGGTTTCATTTTTGAAGTCAAATGAAACATATAGGTTCAGCGGAGGGTTTGTTGAACTAAGGAAAGGATATGGGCTGAAGGTGGGAAACAAATGCAAGCTTTCGTACCAAGGAAACTGGGTATTTGTGATGGAGAAGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

275

Amino Acids

30.54

Weight (kDa)

7.55

Isoelectric Point (pI)

53.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 201 - 267 1.1e-06 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 357
AccB1I GGYRCC 1 cut(s) 52
AciI CCGC 1 cut(s) 726
AclWI GGATC 2 cut(s) 179, 568
AcoI YGGCCR 1 cut(s) 25
AcuI CTGAAG 1 cut(s) 782
AdeI CACNNNGTG 1 cut(s) 86
AfaI GTAC 4 cut(s) 153, 461, 549, 791
AfiI CCNNNNNNNGG 2 cut(s) 527, 638
AgsI TTSAA 4 cut(s) 223, 366, 700, 740
AjiI CACGTC 1 cut(s) 86
AjnI CCWGG 2 cut(s) 14, 130
AluBI AGCT 3 cut(s) 38, 536, 784
AluI AGCT 3 cut(s) 38, 536, 784
Alw21I GWGCWC 1 cut(s) 40
Alw26I GTCTC 2 cut(s) 58, 71
AlwI GGATC 2 cut(s) 179, 568
Ama87I CYCGRG 1 cut(s) 631
AoxI GGCC 1 cut(s) 25
AsuC2I CCSGG 2 cut(s) 632, 633
AsuHPI GGTGA 4 cut(s) 58, 322, 421, 461
AvaI CYCGRG 1 cut(s) 631
BanI GGYRCC 1 cut(s) 52
BanII GRGCYC 1 cut(s) 40
Bbv12I GWGCWC 1 cut(s) 40
BccI CCATC 7 cut(s) 37, 115, 269, 379, 518, 665, 808
BceAI ACGGC 2 cut(s) 66, 422
BciT130I CCWGG 2 cut(s) 16, 132
BcnI CCSGG 2 cut(s) 632, 633
BcoDI GTCTC 2 cut(s) 58, 71
BmcAI AGTACT 1 cut(s) 153
Bme1390I CCNGG 4 cut(s) 16, 132, 632, 633
BmeT110I CYCGRG 1 cut(s) 631
BmgBI CACGTC 1 cut(s) 86
BmiI GGNNCC 1 cut(s) 54
BmrFI CCNGG 4 cut(s) 16, 132, 632, 633
BmrI ACTGGG 1 cut(s) 811
BmsI GCATC 1 cut(s) 442
BmuI ACTGGG 1 cut(s) 811
BpmI CTGGAG 1 cut(s) 687
BpuMI CCSGG 2 cut(s) 632, 633
BsaAI YACGTR 1 cut(s) 257
BsaBI GATNNNNATC 1 cut(s) 357
BsaJI CCNNGG 5 cut(s) 56, 178, 606, 631, 793
BsaXI ACNNNNNCTCC 3 cut(s) 720, 750, 809
Bsc4I CCNNNNNNNGG 2 cut(s) 527, 638
Bse118I RCCGGY 1 cut(s) 27
Bse1I ACTGG 3 cut(s) 159, 670, 806
Bse3DI GCAATG 2 cut(s) 256, 404
Bse8I GATNNNNATC 1 cut(s) 357
BseBI CCWGG 2 cut(s) 16, 132
BseDI CCNNGG 5 cut(s) 56, 178, 606, 631, 793
BseGI GGATG 2 cut(s) 246, 280
BseJI GATNNNNATC 1 cut(s) 357
BseLI CCNNNNNNNGG 2 cut(s) 527, 638
BseMI GCAATG 2 cut(s) 256, 404
BseNI ACTGG 3 cut(s) 159, 670, 806
BseRI GAGGAG 4 cut(s) 89, 294, 521, 543
BshFI GGCC 1 cut(s) 27
BshNI GGYRCC 1 cut(s) 52
BsiHKAI GWGCWC 1 cut(s) 40
BsiHKCI CYCGRG 1 cut(s) 631
BsiSI CCGG 2 cut(s) 28, 632
BslI CCNNNNNNNGG 2 cut(s) 527, 638
BsmAI GTCTC 2 cut(s) 58, 71
BsmI GAATGC 1 cut(s) 439
BsnI GGCC 1 cut(s) 27
BsoBI CYCGRG 1 cut(s) 631
Bsp1286I GDGCHC 1 cut(s) 40
Bsp143I GATC 3 cut(s) 171, 514, 573
BspACI CCGC 1 cut(s) 726
BspANI GGCC 1 cut(s) 27
BspHI TCATGA 1 cut(s) 475
BspLI GGNNCC 1 cut(s) 54
BspPI GGATC 2 cut(s) 179, 568
BspT107I GGYRCC 1 cut(s) 52
BsrDI GCAATG 2 cut(s) 256, 404
BsrFI RCCGGY 1 cut(s) 27
BsrI ACTGG 3 cut(s) 159, 670, 806
BssAI RCCGGY 1 cut(s) 27
BssECI CCNNGG 5 cut(s) 56, 178, 606, 631, 793
BssMI GATC 3 cut(s) 171, 514, 573
BssT1I CCWWGG 2 cut(s) 56, 793
Bst2UI CCWGG 2 cut(s) 16, 132
Bst4CI ACNGT 3 cut(s) 120, 382, 552
Bst6I CTCTTC 1 cut(s) 498
BstAPI GCANNNNNTGC 1 cut(s) 342
BstBAI YACGTR 1 cut(s) 257
BstC8I GCNNGC 1 cut(s) 782
BstDEI CTNAG 1 cut(s) 743
BstDSI CCRYGG 2 cut(s) 178, 606
BstENI CCTNNNNNAGG 1 cut(s) 525
BstF5I GGATG 2 cut(s) 246, 280
BstKTI GATC 3 cut(s) 174, 517, 576
BstMAI GTCTC 2 cut(s) 58, 71
BstMBI GATC 3 cut(s) 171, 514, 573
BstMWI GCNNNNNNNGC 1 cut(s) 342
BstNI CCWGG 2 cut(s) 16, 132
BstSCI CCNGG 4 cut(s) 14, 130, 630, 631
BstX2I RGATCY 1 cut(s) 171
BstYI RGATCY 1 cut(s) 171
BsuRI GGCC 1 cut(s) 27
BtgI CCRYGG 2 cut(s) 178, 606
BtrI CACGTC 1 cut(s) 86
BtsCI GGATG 2 cut(s) 246, 280
Cac8I GCNNGC 1 cut(s) 782
CciI TCATGA 1 cut(s) 475
Cfr10I RCCGGY 1 cut(s) 27
Cfr9I CCCGGG 1 cut(s) 631
Csp6I GTAC 4 cut(s) 152, 460, 548, 790
CviAII CATG 2 cut(s) 476, 681
CviJI RGCY 6 cut(s) 27, 38, 520, 536, 760, 784
CviKI_1 RGCY 6 cut(s) 27, 38, 520, 536, 760, 784
CviQI GTAC 4 cut(s) 152, 460, 548, 790
DdeI CTNAG 1 cut(s) 743
DpnI GATC 3 cut(s) 173, 516, 575
DpnII GATC 3 cut(s) 171, 514, 573
DraIII CACNNNGTG 1 cut(s) 86
EaeI YGGCCR 1 cut(s) 25
Eam1104I CTCTTC 1 cut(s) 498
EarI CTCTTC 1 cut(s) 498
Ecl136II GAGCTC 1 cut(s) 38
Eco130I CCWWGG 2 cut(s) 56, 793
Eco24I GRGCYC 1 cut(s) 40
Eco53kI GAGCTC 1 cut(s) 38
Eco57I CTGAAG 1 cut(s) 782
Eco88I CYCGRG 1 cut(s) 631
EcoICRI GAGCTC 1 cut(s) 38
EcoNI CCTNNNNNAGG 1 cut(s) 525
EcoRII CCWGG 2 cut(s) 14, 130
EcoT14I CCWWGG 2 cut(s) 56, 793
EcoT38I GRGCYC 1 cut(s) 40
ErhI CCWWGG 2 cut(s) 56, 793
FaeI CATG 2 cut(s) 479, 684
FatI CATG 2 cut(s) 475, 680
FauNDI CATATG 1 cut(s) 543
FokI GGATG 2 cut(s) 253, 287
FriOI GRGCYC 1 cut(s) 40
GsuI CTGGAG 1 cut(s) 687
HaeIII GGCC 1 cut(s) 27
HapII CCGG 2 cut(s) 28, 632
Hin1II CATG 2 cut(s) 479, 684
HindIII AAGCTT 1 cut(s) 782
HinfI GANTC 2 cut(s) 282, 494
HpaII CCGG 2 cut(s) 28, 632
HphI GGTGA 4 cut(s) 58, 322, 421, 461
Hpy188I TCNGA 3 cut(s) 42, 499, 578
Hpy188III TCNNGA 3 cut(s) 33, 476, 488
Hpy99I CGWCG 1 cut(s) 87
HpyAV CCTTC 1 cut(s) 757
HpyCH4III ACNGT 3 cut(s) 120, 382, 552
HpyCH4IV ACGT 3 cut(s) 85, 256, 462
HpyCH4V TGCA 9 cut(s) 116, 249, 254, 317, 336, 345, 397, 617, 780
HpyF10VI GCNNNNNNNGC 1 cut(s) 342
HpyF3I CTNAG 1 cut(s) 743
HpySE526I ACGT 3 cut(s) 85, 256, 462
Hsp92II CATG 2 cut(s) 479, 684
Kzo9I GATC 3 cut(s) 171, 514, 573
LmnI GCTCC 1 cut(s) 35
LweI GCATC 1 cut(s) 442
MaeII ACGT 3 cut(s) 85, 256, 462
MaeIII GTNAC 2 cut(s) 46, 439
MalI GATC 3 cut(s) 173, 516, 575
MboI GATC 3 cut(s) 171, 514, 573
MboII GAAGA 4 cut(s) 166, 491, 515, 524
MflI RGATCY 1 cut(s) 171
MhlI GDGCHC 1 cut(s) 40
MluCI AATT 2 cut(s) 232, 589
MlyI GAGTC 1 cut(s) 291
MspA1I CMGCKG 1 cut(s) 726
MspI CCGG 2 cut(s) 28, 632
MspR9I CCNGG 4 cut(s) 16, 132, 632, 633
Mva1269I GAATGC 1 cut(s) 439
MvaI CCWGG 2 cut(s) 16, 132
MwoI GCNNNNNNNGC 1 cut(s) 342
NciI CCSGG 2 cut(s) 632, 633
NdeI CATATG 1 cut(s) 543
NdeII GATC 3 cut(s) 171, 514, 573
NlaIII CATG 2 cut(s) 479, 684
NlaIV GGNNCC 1 cut(s) 54
NmuCI GTSAC 1 cut(s) 46
PagI TCATGA 1 cut(s) 475
PctI GAATGC 1 cut(s) 439
PfeI GAWTC 1 cut(s) 494
PleI GAGTC 1 cut(s) 290
PpsI GAGTC 1 cut(s) 290
Ppu21I YACGTR 1 cut(s) 257
PsiI TTATAA 1 cut(s) 357
Psp124BI GAGCTC 1 cut(s) 40
Psp6I CCWGG 2 cut(s) 14, 130
PspGI CCWGG 2 cut(s) 14, 130
PspN4I GGNNCC 1 cut(s) 54
PsuI RGATCY 1 cut(s) 171
RsaI GTAC 4 cut(s) 153, 461, 549, 791
RsaNI GTAC 4 cut(s) 152, 460, 548, 790
SacI GAGCTC 1 cut(s) 40
Sau3AI GATC 3 cut(s) 171, 514, 573
ScaI AGTACT 1 cut(s) 153
SchI GAGTC 1 cut(s) 291
ScrFI CCNGG 4 cut(s) 16, 132, 632, 633
SduI GDGCHC 1 cut(s) 40
SfaNI GCATC 1 cut(s) 442
SmaI CCCGGG 1 cut(s) 633
Sse9I AATT 2 cut(s) 232, 589
SsiI CCGC 1 cut(s) 726
SspI AATATT 1 cut(s) 626
SstI GAGCTC 1 cut(s) 40
StyD4I CCNGG 4 cut(s) 14, 130, 630, 631
StyI CCWWGG 2 cut(s) 56, 793
TaaI ACNGT 3 cut(s) 120, 382, 552
TaiI ACGT 3 cut(s) 88, 259, 465
TaqI TCGA 2 cut(s) 193, 416
TasI AATT 2 cut(s) 232, 589
TatI WGTACW 1 cut(s) 151
TfiI GAWTC 1 cut(s) 494
TseFI GTSAC 1 cut(s) 46
Tsp45I GTSAC 1 cut(s) 46
TspDTI ATGAA 6 cut(s) 155, 245, 446, 492, 681, 723
TspGWI ACGGA 2 cut(s) 167, 623
TspMI CCCGGG 1 cut(s) 631
XagI CCTNNNNNAGG 1 cut(s) 525
XmaI CCCGGG 1 cut(s) 631
ZrmI AGTACT 1 cut(s) 153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.