Rw1G010450

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0002876
Physical Location & Seq
Forward (+)
23378882 .. 23381361
2480 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G010450.1

Sequence Viewer

Length: 1044 bp
ATGGCTTCAGGAAGTGGATCACACCGTCCAATGCAGTTGGGAAGGCAGCCGTGTTTCATCCAAGTTGTGAGGAGCCCGTCAAAGAACACGGAGAGTTTGGTCATCCCTCCTCCATTTGTGATAGAGTTCATGAATGGTGACAGATGCCGGCAGGGAAAGATAAAACTGGAAGGATTGGAACAGTGCCAGTGGCGGGTTGAAATAAATACGGATGGGAGGGGAGTTGAATTGGGTGAAGGGTGGGCGGAATTTGTGGGGGAAAATTTGGAGGACGGGTGGATGCTAGTGCTGTTAAGGTACGAGGGGGCAATGACTTTTTCTGCCAGGAGATATGGCCGGTCAGGAGCTCTGATGGTGACGGCACCAAGGGGGAGACAGACAGAGGAGACACCACGTGGAGTTAGGGGCATTTATCCATCATTTGCACAGTTGGTTATTCGAGGTGGAATGAACAGTGAAGTACTGTGCAACGCACGTATCAAGGTAAGTGAGGATGGAGGAGTCATAAAAGGAGGTTGGGACAGGTGGGTGAGTGCAAACATTGTAAGGGTATGCAAGTGGTGCATTGTGGATTGTAATCGTTCAAAAAATGAGGCAACTGTGATGGCGTTTCTGCAATGTGGAGGGGTGATGTCGAACGTGGCAATGATGAATGCCGTAACAATCGGTGATGCTATGTACGTTGATAGTTGTGATGAAGAATATCCAGATGATTCAGAGGAAGAGGAGGAAGATCTGCTTGATGAAGGAGAAGCTGGTCATATGGTACAGTTTCCAATACCTCAAACTGTGATCCAAGTTGGAAAAAAATATGGGCAACTTCCCACAGAGTATGCAAAGCAATATTTCCCGGGTGTCGGTAGTCGTCAGGTTGTTGTGAGGACTGGAGATGGACAATCATGGGAGGGTGATGTCATGTTGCAGCTGCTTCAAGTTAGCGGTAACTCAATCCTGTATGGGTATGCGGAAAAATTCGCCATAGTGTTCCACAATTTAAAACTGGAGACCACATGGTGGAGCCGTGGAGGAGATGAGAACCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

347

Amino Acids

38.66

Weight (kDa)

5.06

Isoelectric Point (pI)

44.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 19 - 106 1.5e-10 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 361
AccB7I CCANNNNNTGG 1 cut(s) 1014
AciI CCGC 4 cut(s) 193, 245, 939, 965
AclWI GGATC 2 cut(s) 25, 787
AcoI YGGCCR 1 cut(s) 334
AcsI RAATTY 3 cut(s) 248, 262, 971
AcvI CACGTG 1 cut(s) 395
AdeI CACNNNGTG 2 cut(s) 395, 1014
AfaI GTAC 4 cut(s) 299, 462, 680, 768
AfiI CCNNNNNNNGG 3 cut(s) 193, 857, 1014
AgsI TTSAA 4 cut(s) 200, 227, 585, 932
AjnI CCWGG 1 cut(s) 323
AluBI AGCT 3 cut(s) 347, 755, 925
AluI AGCT 3 cut(s) 347, 755, 925
Alw21I GWGCWC 1 cut(s) 349
Alw26I GTCTC 3 cut(s) 367, 380, 998
AlwI GGATC 2 cut(s) 25, 787
Ama87I CYCGRG 1 cut(s) 850
AoxI GGCC 1 cut(s) 334
ApeKI GCWGC 3 cut(s) 46, 922, 925
ApoI RAATTY 3 cut(s) 248, 262, 971
AsuC2I CCSGG 2 cut(s) 851, 852
AsuHPI GGTGA 7 cut(s) 149, 245, 367, 541, 640, 680, 920
AvaI CYCGRG 1 cut(s) 850
BaeI ACNNNNGTAYC 2 cut(s) 460, 493
BanI GGYRCC 1 cut(s) 361
BanII GRGCYC 2 cut(s) 77, 349
BbrPI CACGTG 1 cut(s) 395
Bbv12I GWGCWC 1 cut(s) 349
BbvI GCAGC 3 cut(s) 58, 912, 934
BccI CCATC 6 cut(s) 206, 346, 424, 488, 598, 884
BceAI ACGGC 4 cut(s) 34, 375, 641, 1005
BciT130I CCWGG 1 cut(s) 325
BcnI CCSGG 2 cut(s) 851, 852
BcoDI GTCTC 3 cut(s) 367, 380, 998
BfaI CTAG 1 cut(s) 284
BglII AGATCT 1 cut(s) 733
BisI GCNGC 3 cut(s) 47, 923, 926
BlsI GCNGC 3 cut(s) 48, 924, 927
BmcAI AGTACT 1 cut(s) 462
Bme1390I CCNGG 3 cut(s) 325, 851, 852
BmeT110I CYCGRG 1 cut(s) 850
BmiI GGNNCC 3 cut(s) 74, 363, 1019
BmrFI CCNGG 3 cut(s) 325, 851, 852
BmsI GCATC 3 cut(s) 134, 270, 661
BpmI CTGGAG 2 cut(s) 906, 1022
BpuMI CCSGG 2 cut(s) 851, 852
BsaAI YACGTR 2 cut(s) 395, 476
BsaBI GATNNNNATC 1 cut(s) 576
BsaI GGTCTC 1 cut(s) 998
BsaJI CCNNGG 3 cut(s) 365, 850, 1021
BsaXI ACNNNNNCTCC 2 cut(s) 260, 290
Bsc4I CCNNNNNNNGG 3 cut(s) 193, 857, 1014
Bse118I RCCGGY 2 cut(s) 147, 336
Bse1I ACTGG 4 cut(s) 171, 187, 889, 1005
Bse3DI GCAATG 3 cut(s) 315, 623, 651
Bse8I GATNNNNATC 1 cut(s) 576
BseBI CCWGG 1 cut(s) 325
BseDI CCNNGG 3 cut(s) 365, 850, 1021
BseGI GGATG 5 cut(s) 57, 102, 217, 285, 499
BseJI GATNNNNATC 1 cut(s) 576
BseLI CCNNNNNNNGG 3 cut(s) 193, 857, 1014
BseMI GCAATG 3 cut(s) 315, 623, 651
BseNI ACTGG 4 cut(s) 171, 187, 889, 1005
BseRI GAGGAG 6 cut(s) 85, 99, 398, 513, 740, 1041
BseXI GCAGC 3 cut(s) 58, 912, 934
BshFI GGCC 1 cut(s) 336
BshNI GGYRCC 1 cut(s) 361
BsiHKAI GWGCWC 1 cut(s) 349
BsiHKCI CYCGRG 1 cut(s) 850
BsiSI CCGG 3 cut(s) 148, 337, 851
BslFI GGGAC 1 cut(s) 533
BslI CCNNNNNNNGG 3 cut(s) 193, 857, 1014
BsmAI GTCTC 3 cut(s) 367, 380, 998
BsmFI GGGAC 1 cut(s) 533
BsmI GAATGC 1 cut(s) 658
BsnI GGCC 1 cut(s) 336
Bso31I GGTCTC 1 cut(s) 998
BsoBI CYCGRG 1 cut(s) 850
Bsp1286I GDGCHC 2 cut(s) 77, 349
Bsp143I GATC 3 cut(s) 17, 733, 792
BspACI CCGC 4 cut(s) 193, 245, 939, 965
BspANI GGCC 1 cut(s) 336
BspHI TCATGA 1 cut(s) 129
BspLI GGNNCC 3 cut(s) 74, 363, 1019
BspPI GGATC 2 cut(s) 25, 787
BspT107I GGYRCC 1 cut(s) 361
BspTNI GGTCTC 1 cut(s) 998
BsrDI GCAATG 3 cut(s) 315, 623, 651
BsrFI RCCGGY 2 cut(s) 147, 336
BsrI ACTGG 4 cut(s) 171, 187, 889, 1005
BssAI RCCGGY 2 cut(s) 147, 336
BssECI CCNNGG 3 cut(s) 365, 850, 1021
BssMI GATC 3 cut(s) 17, 733, 792
BssT1I CCWWGG 1 cut(s) 365
Bst2UI CCWGG 1 cut(s) 325
Bst4CI ACNGT 9 cut(s) 26, 183, 429, 455, 465, 601, 771, 790, 1040
Bst6I CTCTTC 1 cut(s) 717
BstAPI GCANNNNNTGC 1 cut(s) 561
BstBAI YACGTR 2 cut(s) 395, 476
BstC8I GCNNGC 1 cut(s) 149
BstDSI CCRYGG 1 cut(s) 1021
BstF5I GGATG 5 cut(s) 57, 102, 217, 285, 499
BstKTI GATC 3 cut(s) 20, 736, 795
BstMAI GTCTC 3 cut(s) 367, 380, 998
BstMBI GATC 3 cut(s) 17, 733, 792
BstMWI GCNNNNNNNGC 1 cut(s) 561
BstNI CCWGG 1 cut(s) 325
BstSCI CCNGG 3 cut(s) 323, 849, 850
BstV1I GCAGC 3 cut(s) 58, 912, 934
BstX2I RGATCY 1 cut(s) 733
BstYI RGATCY 1 cut(s) 733
BsuRI GGCC 1 cut(s) 336
BtgI CCRYGG 1 cut(s) 1021
BtsCI GGATG 5 cut(s) 57, 102, 217, 285, 499
BtsIMutI CAGTG 3 cut(s) 188, 194, 460
Cac8I GCNNGC 1 cut(s) 149
CciI TCATGA 1 cut(s) 129
Cfr10I RCCGGY 2 cut(s) 147, 336
Cfr9I CCCGGG 1 cut(s) 850
Csp6I GTAC 4 cut(s) 298, 461, 679, 767
CviAII CATG 4 cut(s) 130, 900, 916, 1011
CviJI RGCY 8 cut(s) 5, 49, 75, 336, 347, 755, 925, 1020
CviKI_1 RGCY 8 cut(s) 5, 49, 75, 336, 347, 755, 925, 1020
CviQI GTAC 4 cut(s) 298, 461, 679, 767
DpnI GATC 3 cut(s) 19, 735, 794
DpnII GATC 3 cut(s) 17, 733, 792
DraI TTTAAA 1 cut(s) 996
DraIII CACNNNGTG 2 cut(s) 395, 1014
EaeI YGGCCR 1 cut(s) 334
Eam1104I CTCTTC 1 cut(s) 717
EarI CTCTTC 1 cut(s) 717
EciI GGCGGA 1 cut(s) 260
Ecl136II GAGCTC 1 cut(s) 347
Eco130I CCWWGG 1 cut(s) 365
Eco24I GRGCYC 2 cut(s) 77, 349
Eco31I GGTCTC 1 cut(s) 998
Eco53kI GAGCTC 1 cut(s) 347
Eco72I CACGTG 1 cut(s) 395
Eco88I CYCGRG 1 cut(s) 850
EcoICRI GAGCTC 1 cut(s) 347
EcoRII CCWGG 1 cut(s) 323
EcoT14I CCWWGG 1 cut(s) 365
EcoT38I GRGCYC 2 cut(s) 77, 349
ErhI CCWWGG 1 cut(s) 365
FaeI CATG 4 cut(s) 133, 903, 919, 1014
FalI AAGNNNNNCTT 2 cut(s) 723, 755
FaqI GGGAC 1 cut(s) 533
FatI CATG 4 cut(s) 129, 899, 915, 1010
FauI CCCGC 1 cut(s) 186
FauNDI CATATG 1 cut(s) 762
Fnu4HI GCNGC 3 cut(s) 47, 923, 926
FokI GGATG 5 cut(s) 44, 89, 224, 292, 506
FriOI GRGCYC 2 cut(s) 77, 349
Fsp4HI GCNGC 3 cut(s) 47, 923, 926
FspBI CTAG 1 cut(s) 284
GluI GCNGC 3 cut(s) 47, 923, 926
GsuI CTGGAG 2 cut(s) 906, 1022
HaeIII GGCC 1 cut(s) 336
HapII CCGG 3 cut(s) 148, 337, 851
Hin1II CATG 4 cut(s) 133, 903, 919, 1014
HinfI GANTC 2 cut(s) 501, 713
HpaII CCGG 3 cut(s) 148, 337, 851
HphI GGTGA 7 cut(s) 149, 245, 367, 541, 640, 680, 920
Hpy188I TCNGA 2 cut(s) 351, 718
Hpy188III TCNNGA 4 cut(s) 9, 130, 342, 707
HpyAV CCTTC 4 cut(s) 36, 164, 230, 740
HpyCH4III ACNGT 9 cut(s) 26, 183, 429, 455, 465, 601, 771, 790, 1040
HpyCH4IV ACGT 4 cut(s) 394, 475, 639, 681
HpyCH4V TGCA 9 cut(s) 34, 425, 468, 536, 555, 564, 616, 836, 922
HpyF10VI GCNNNNNNNGC 1 cut(s) 561
HpySE526I ACGT 4 cut(s) 394, 475, 639, 681
Hsp92II CATG 4 cut(s) 133, 903, 919, 1014
KroI GCCGGC 1 cut(s) 147
KroNI GCCGGC 1 cut(s) 149
Kzo9I GATC 3 cut(s) 17, 733, 792
LmnI GCTCC 3 cut(s) 72, 344, 1017
Lsp1109I GCAGC 3 cut(s) 58, 912, 934
LweI GCATC 3 cut(s) 134, 270, 661
MaeI CTAG 1 cut(s) 284
MaeII ACGT 4 cut(s) 394, 475, 639, 681
MaeIII GTNAC 4 cut(s) 137, 355, 658, 941
MalI GATC 3 cut(s) 19, 735, 794
MboI GATC 3 cut(s) 17, 733, 792
MboII GAAGA 3 cut(s) 710, 734, 743
MflI RGATCY 1 cut(s) 733
MhlI GDGCHC 2 cut(s) 77, 349
MluCI AATT 5 cut(s) 227, 248, 262, 971, 991
MlyI GAGTC 1 cut(s) 510
MmeI TCCRAC 1 cut(s) 781
MroNI GCCGGC 1 cut(s) 147
MseI TTAA 2 cut(s) 293, 995
MspA1I CMGCKG 1 cut(s) 925
MspI CCGG 3 cut(s) 148, 337, 851
MspR9I CCNGG 3 cut(s) 325, 851, 852
Mva1269I GAATGC 1 cut(s) 658
MvaI CCWGG 1 cut(s) 325
MwoI GCNNNNNNNGC 1 cut(s) 561
NaeI GCCGGC 1 cut(s) 149
NciI CCSGG 2 cut(s) 851, 852
NdeI CATATG 1 cut(s) 762
NdeII GATC 3 cut(s) 17, 733, 792
NgoMIV GCCGGC 1 cut(s) 147
NlaIII CATG 4 cut(s) 133, 903, 919, 1014
NlaIV GGNNCC 3 cut(s) 74, 363, 1019
NmuCI GTSAC 2 cut(s) 137, 355
PagI TCATGA 1 cut(s) 129
PctI GAATGC 1 cut(s) 658
PdiI GCCGGC 1 cut(s) 149
PfeI GAWTC 1 cut(s) 713
PflMI CCANNNNNTGG 1 cut(s) 1014
PkrI GCNGC 3 cut(s) 48, 924, 927
PleI GAGTC 1 cut(s) 509
PmaCI CACGTG 1 cut(s) 395
PmlI CACGTG 1 cut(s) 395
PpsI GAGTC 1 cut(s) 509
Ppu21I YACGTR 2 cut(s) 395, 476
Psp124BI GAGCTC 1 cut(s) 349
Psp6I CCWGG 1 cut(s) 323
PspCI CACGTG 1 cut(s) 395
PspGI CCWGG 1 cut(s) 323
PspN4I GGNNCC 3 cut(s) 74, 363, 1019
PsuI RGATCY 1 cut(s) 733
PvuII CAGCTG 1 cut(s) 925
RsaI GTAC 4 cut(s) 299, 462, 680, 768
RsaNI GTAC 4 cut(s) 298, 461, 679, 767
SacI GAGCTC 1 cut(s) 349
SaqAI TTAA 2 cut(s) 293, 995
SatI GCNGC 3 cut(s) 47, 923, 926
Sau3AI GATC 3 cut(s) 17, 733, 792
ScaI AGTACT 1 cut(s) 462
SchI GAGTC 1 cut(s) 510
ScrFI CCNGG 3 cut(s) 325, 851, 852
SduI GDGCHC 2 cut(s) 77, 349
SfaNI GCATC 3 cut(s) 134, 270, 661
SmaI CCCGGG 1 cut(s) 852
Sse9I AATT 5 cut(s) 227, 248, 262, 971, 991
SsiI CCGC 4 cut(s) 193, 245, 939, 965
SspI AATATT 1 cut(s) 845
SspMI CTAG 1 cut(s) 284
SstI GAGCTC 1 cut(s) 349
StyD4I CCNGG 3 cut(s) 323, 849, 850
StyI CCWWGG 1 cut(s) 365
TaaI ACNGT 9 cut(s) 26, 183, 429, 455, 465, 601, 771, 790, 1040
TaiI ACGT 4 cut(s) 397, 478, 642, 684
TaqI TCGA 2 cut(s) 439, 635
TasI AATT 5 cut(s) 227, 248, 262, 971, 991
TatI WGTACW 1 cut(s) 460
TfiI GAWTC 1 cut(s) 713
Tru1I TTAA 2 cut(s) 293, 995
Tru9I TTAA 2 cut(s) 293, 995
TscAI CASTG 3 cut(s) 188, 194, 460
TseFI GTSAC 2 cut(s) 137, 355
TseI GCWGC 3 cut(s) 46, 922, 925
Tsp45I GTSAC 2 cut(s) 137, 355
TspDTI ATGAA 7 cut(s) 46, 118, 146, 464, 665, 711, 759
TspGWI ACGGA 2 cut(s) 104, 224
TspMI CCCGGG 1 cut(s) 850
TspRI CASTG 3 cut(s) 188, 194, 460
Van91I CCANNNNNTGG 1 cut(s) 1014
XapI RAATTY 3 cut(s) 248, 262, 971
XmaI CCCGGG 1 cut(s) 850
XspI CTAG 1 cut(s) 284
ZrmI AGTACT 1 cut(s) 462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.