Rmu_ssc0000434.1_g000011

invertase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000434.1
OG0001702
Physical Location & Seq
Forward (+)
52297 .. 57658
5362 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1335 bp
atggatgacgctgctgaggttgagcgggtcggtgcggtagccacaaaggtggaaggcttggtcgacattgcggtggcaggtgcagaagaagaagattgcgaggacttgatcagcggtgggagaagaaaacgaaggatcagtgcgatcaagtttgtcggcggagctggagaagaggcggcgtctgagtcgatgtcggcactgaaagtcgttcgccgtggtgaacttagtggaacagatcaacctggaggtacccagcgctctcgatctttctcggtgcttgatgacccgcgaccaagcccgaacgtattctcgctgtccggccacctcacgacggtgcaccaccaccaatggcttcctctcctcgtcgccaacgtctctctggggtcaggtctttcaaccggaagctctggcgcgagaatcgacaccatgaagcctcatgttttccgacgagtttctacaaattccagtgaccgattgggctgcatgtgttctgggttgctctcggactcgacgctgtcaccgacgccggtgactcctccggcacctctggccttggattccgtcttacctgcatgtatggatgcgtatttcagaagctgtgaatgggtggaagtcgatttggcaatatggtactacgtgcttatccaaactacctataaatacccctacctcgcaaaacttcatagttctcatccccacaaaatgacaactcccatattcaccttcacattcctccctctctttctcctcatctcatcaaccttcactcccataactgccaacaatctcatctctgaaacctgcaagaaatgtgctcggaccgaccccaacctgagctacaagttctgcgtgacttctctccaagccgccccaaacagccacagagccgatctccggcgactcggcctcatctccatgaagctagtccagcacaacgtgaccaacacgaggcacttcatcaagcatcttctcaagaacaagaagcttgacaagttcgtaagggcgcgtttggatgactgtttggagctttacacagacgccatacccaccatcaagcaagccatcaagaattacaaaaagaagcacaacaaagacgccaatatcgaagtgagttcgatcgtcgatgcctccacgacttgcgaagatggattcgaggagagaccagggctggtttcgccattaacgaagaggaacaaagagacgtttcagctgtgtattatagcgctttcgattatcaatatggtgactcaaaaagtgttgtttatctttatgggttcccatccattctatgttttgaagcacgggacgacctcatcggctcatctccgctcttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

444

Amino Acids

48.97

Weight (kDa)

8.93

Isoelectric Point (pI)

46.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 262 - 411 9.3e-28 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 68
Acc36I ACCTGC 3 cut(s) 68, 577, 809
Acc65I GGTACC 1 cut(s) 248
AccB1I GGYRCC 2 cut(s) 248, 541
AccBSI CCGCTC 2 cut(s) 25, 1329
AccI GTMKAC 1 cut(s) 63
AccII CGCG 3 cut(s) 289, 413, 1006
AciI CCGC 9 cut(s) 25, 35, 71, 114, 159, 176, 287, 867, 1327
AclWI GGATC 1 cut(s) 143
AcoI YGGCCR 1 cut(s) 319
AcsI RAATTY 1 cut(s) 460
AcyI GRCGYC 4 cut(s) 179, 524, 1038, 1095
AdeI CACNNNGTG 1 cut(s) 937
AfaI GTAC 2 cut(s) 250, 632
AfeI AGCGCT 2 cut(s) 257, 1224
AfiI CCNNNNNNNGG 3 cut(s) 331, 894, 948
AgsI TTSAA 2 cut(s) 396, 1297
AjnI CCWGG 2 cut(s) 241, 1162
AleI CACNNNNGTG 2 cut(s) 47, 332
AluBI AGCT 8 cut(s) 164, 405, 597, 837, 922, 985, 1027, 1210
AluI AGCT 8 cut(s) 164, 405, 597, 837, 922, 985, 1027, 1210
Alw21I GWGCWC 2 cut(s) 339, 817
Alw26I GTCTC 3 cut(s) 379, 1153, 1193
Alw44I GTGCAC 1 cut(s) 335
AlwI GGATC 1 cut(s) 143
AlwNI CAGNNNCTG 1 cut(s) 597
Aor51HI AGCGCT 2 cut(s) 257, 1224
AoxI GGCC 3 cut(s) 319, 549, 904
ApaLI GTGCAC 1 cut(s) 335
ApeKI GCWGC 2 cut(s) 11, 480
ApoI RAATTY 1 cut(s) 460
Asp700I GAANNNNTTC 1 cut(s) 305
Asp718I GGTACC 1 cut(s) 248
AspLEI GCGC 4 cut(s) 258, 413, 1006, 1225
AspS9I GGNCC 1 cut(s) 819
AsuHPI GGTGA 5 cut(s) 230, 510, 541, 712, 1255
AvaII GGWCC 1 cut(s) 819
BaeGI GKGCMC 1 cut(s) 339
BanI GGYRCC 2 cut(s) 248, 541
BauI CACGAG 1 cut(s) 946
Bbv12I GWGCWC 2 cut(s) 339, 817
BbvCI CCTCAGC 1 cut(s) 15
BbvI GCAGC 1 cut(s) 467
BccI CCATC 4 cut(s) 1058, 1070, 1139, 1287
BceAI ACGGC 1 cut(s) 198
BcgI CGANNNNNNTGC 2 cut(s) 462, 496
BciT130I CCWGG 2 cut(s) 243, 1164
BclI TGATCA 1 cut(s) 108
BcoDI GTCTC 3 cut(s) 379, 1153, 1193
BfaI CTAG 1 cut(s) 923
BfoI RGCGCY 2 cut(s) 259, 1226
BfuAI ACCTGC 3 cut(s) 68, 577, 809
BisI GCNGC 4 cut(s) 12, 177, 481, 867
BlsI GCNGC 4 cut(s) 13, 178, 482, 868
Bme1390I CCNGG 2 cut(s) 243, 1164
Bme18I GGWCC 1 cut(s) 819
BmgT120I GGNCC 1 cut(s) 819
BmiI GGNNCC 3 cut(s) 250, 543, 1276
BmrFI CCNGG 2 cut(s) 243, 1164
BmsI GCATC 3 cut(s) 571, 973, 1114
BplI GAGNNNNNCTC 2 cut(s) 876, 908
BpmI CTGGAG 2 cut(s) 186, 264
Bpu10I CCTNAGC 2 cut(s) 15, 833
BpuEI CTTGAG 1 cut(s) 956
BsaAI YACGTR 1 cut(s) 637
BsaHI GRCGYC 4 cut(s) 179, 524, 1038, 1095
BsaI GGTCTC 1 cut(s) 1153
BsaJI CCNNGG 3 cut(s) 214, 552, 1163
BsaWI WCCGGW 1 cut(s) 398
BsaXI ACNNNNNCTCC 2 cut(s) 751, 781
Bsc4I CCNNNNNNNGG 3 cut(s) 331, 894, 948
Bse118I RCCGGY 1 cut(s) 526
Bse1I ACTGG 1 cut(s) 465
Bse3DI GCAATG 1 cut(s) 66
BseBI CCWGG 2 cut(s) 243, 1164
BseDI CCNNGG 3 cut(s) 214, 552, 1163
BseGI GGATG 5 cut(s) 10, 586, 691, 1018, 1279
BseLI CCNNNNNNNGG 3 cut(s) 331, 894, 948
BseMI GCAATG 1 cut(s) 66
BseMII CTCAG 3 cut(s) 6, 174, 824
BseNI ACTGG 1 cut(s) 465
BseRI GAGGAG 4 cut(s) 350, 525, 737, 1169
BseSI GKGCMC 1 cut(s) 339
BseXI GCAGC 1 cut(s) 467
BseYI CCCAGC 1 cut(s) 252
BsgI GTGCAG 1 cut(s) 102
Bsh1236I CGCG 3 cut(s) 289, 413, 1006
Bsh1285I CGRYCG 1 cut(s) 1119
BshFI GGCC 3 cut(s) 321, 551, 906
BshNI GGYRCC 2 cut(s) 248, 541
BsiEI CGRYCG 1 cut(s) 1119
BsiHKAI GWGCWC 2 cut(s) 339, 817
BsiSI CCGG 5 cut(s) 318, 399, 527, 539, 895
BslFI GGGAC 1 cut(s) 1318
BslI CCNNNNNNNGG 3 cut(s) 331, 894, 948
BsmAI GTCTC 3 cut(s) 379, 1153, 1193
BsmBI CGTCTC 2 cut(s) 379, 1193
BsmFI GGGAC 1 cut(s) 1318
BsnI GGCC 3 cut(s) 321, 551, 906
Bso31I GGTCTC 1 cut(s) 1153
Bsp1286I GDGCHC 2 cut(s) 339, 817
Bsp143I GATC 7 cut(s) 108, 135, 144, 235, 263, 889, 1116
BspACI CCGC 9 cut(s) 25, 35, 71, 114, 159, 176, 287, 867, 1327
BspANI GGCC 3 cut(s) 321, 551, 906
BspCNI CTCAG 3 cut(s) 7, 175, 825
BspFNI CGCG 3 cut(s) 289, 413, 1006
BspLI GGNNCC 3 cut(s) 250, 543, 1276
BspMI ACCTGC 3 cut(s) 68, 577, 809
BspPI GGATC 1 cut(s) 143
BspT107I GGYRCC 2 cut(s) 248, 541
BspTNI GGTCTC 1 cut(s) 1153
BsrBI CCGCTC 2 cut(s) 25, 1329
BsrDI GCAATG 1 cut(s) 66
BsrFI RCCGGY 1 cut(s) 526
BsrI ACTGG 1 cut(s) 465
BssAI RCCGGY 1 cut(s) 526
BssECI CCNNGG 3 cut(s) 214, 552, 1163
BssMI GATC 7 cut(s) 108, 135, 144, 235, 263, 889, 1116
BssNI GRCGYC 4 cut(s) 179, 524, 1038, 1095
BssSI CACGAG 1 cut(s) 946
BssT1I CCWWGG 1 cut(s) 552
Bst2BI CACGAG 1 cut(s) 946
Bst2UI CCWGG 2 cut(s) 243, 1164
Bst4CI ACNGT 2 cut(s) 334, 1019
Bst6I CTCTTC 2 cut(s) 165, 1181
BstACI GRCGYC 4 cut(s) 179, 524, 1038, 1095
BstBAI YACGTR 1 cut(s) 637
BstC8I GCNNGC 1 cut(s) 1059
BstDEI CTNAG 4 cut(s) 15, 183, 224, 833
BstDSI CCRYGG 1 cut(s) 214
BstF5I GGATG 5 cut(s) 10, 586, 691, 1018, 1279
BstFNI CGCG 3 cut(s) 289, 413, 1006
BstH2I RGCGCY 2 cut(s) 259, 1226
BstHHI GCGC 4 cut(s) 258, 413, 1006, 1225
BstKTI GATC 7 cut(s) 111, 138, 147, 238, 266, 892, 1119
BstMAI GTCTC 3 cut(s) 379, 1153, 1193
BstMBI GATC 7 cut(s) 108, 135, 144, 235, 263, 889, 1116
BstMCI CGRYCG 1 cut(s) 1119
BstMWI GCNNNNNNNGC 3 cut(s) 548, 928, 1174
BstNI CCWGG 2 cut(s) 243, 1164
BstNSI RCATGY 2 cut(s) 487, 576
BstSCI CCNGG 2 cut(s) 241, 1162
BstSLI GKGCMC 1 cut(s) 339
BstUI CGCG 3 cut(s) 289, 413, 1006
BstV1I GCAGC 1 cut(s) 467
BstXI CCANNNNNNTGG 1 cut(s) 49
BsuRI GGCC 3 cut(s) 321, 551, 906
BtgI CCRYGG 1 cut(s) 214
BtsCI GGATG 5 cut(s) 10, 586, 691, 1018, 1279
BtsIMutI CAGTG 3 cut(s) 145, 197, 472
BveI ACCTGC 3 cut(s) 68, 577, 809
Cac8I GCNNGC 1 cut(s) 1059
CaiI CAGNNNCTG 1 cut(s) 597
CfoI GCGC 4 cut(s) 258, 413, 1006, 1225
Cfr10I RCCGGY 1 cut(s) 526
Cfr13I GGNCC 1 cut(s) 819
CpoI CGGWCCG 1 cut(s) 819
CseI GACGC 6 cut(s) 17, 168, 520, 532, 1046, 1103
Csp6I GTAC 2 cut(s) 249, 631
CspI CGGWCCG 1 cut(s) 819
CviAII CATG 5 cut(s) 427, 437, 484, 573, 916
CviQI GTAC 2 cut(s) 249, 631
DdeI CTNAG 4 cut(s) 15, 183, 224, 833
DpnI GATC 7 cut(s) 110, 137, 146, 237, 265, 891, 1118
DpnII GATC 7 cut(s) 108, 135, 144, 235, 263, 889, 1116
DraIII CACNNNGTG 1 cut(s) 937
EaeI YGGCCR 1 cut(s) 319
Eam1104I CTCTTC 2 cut(s) 165, 1181
EarI CTCTTC 2 cut(s) 165, 1181
EciI GGCGGA 1 cut(s) 174
Eco130I CCWWGG 1 cut(s) 552
Eco31I GGTCTC 1 cut(s) 1153
Eco47I GGWCC 1 cut(s) 819
Eco47III AGCGCT 2 cut(s) 257, 1224
EcoRII CCWGG 2 cut(s) 241, 1162
EcoT14I CCWWGG 1 cut(s) 552
ErhI CCWWGG 1 cut(s) 552
Esp3I CGTCTC 2 cut(s) 379, 1193
FaeI CATG 5 cut(s) 430, 440, 487, 576, 919
FaqI GGGAC 1 cut(s) 1318
FatI CATG 5 cut(s) 426, 436, 483, 572, 915
FauI CCCGC 2 cut(s) 18, 294
FbaI TGATCA 1 cut(s) 108
FblI GTMKAC 1 cut(s) 63
Fnu4HI GCNGC 4 cut(s) 12, 177, 481, 867
FokI GGATG 5 cut(s) 17, 593, 678, 1025, 1266
Fsp4HI GCNGC 4 cut(s) 12, 177, 481, 867
FspBI CTAG 1 cut(s) 923
GlaI GCGC 4 cut(s) 257, 412, 1005, 1224
GluI GCNGC 4 cut(s) 12, 177, 481, 867
GsaI CCCAGC 1 cut(s) 256
GsuI CTGGAG 2 cut(s) 186, 264
HaeII RGCGCY 2 cut(s) 259, 1226
HaeIII GGCC 3 cut(s) 321, 551, 906
HapII CCGG 5 cut(s) 318, 399, 527, 539, 895
HgaI GACGC 6 cut(s) 17, 168, 520, 532, 1046, 1103
HhaI GCGC 4 cut(s) 258, 413, 1006, 1225
Hin1I GRCGYC 4 cut(s) 179, 524, 1038, 1095
Hin1II CATG 5 cut(s) 430, 440, 487, 576, 919
Hin6I GCGC 4 cut(s) 256, 411, 1004, 1223
HinP1I GCGC 4 cut(s) 256, 411, 1004, 1223
HincII GTYRAC 1 cut(s) 64
HindII GTYRAC 1 cut(s) 64
HindIII AAGCTT 1 cut(s) 983
HinfI GANTC 8 cut(s) 185, 417, 506, 532, 557, 900, 1149, 1246
HpaII CCGG 5 cut(s) 318, 399, 527, 539, 895
HphI GGTGA 5 cut(s) 230, 510, 541, 712, 1255
Hpy166II GTNNAC 3 cut(s) 64, 221, 337
Hpy188I TCNGA 6 cut(s) 184, 446, 505, 593, 796, 819
Hpy188III TCNNGA 5 cut(s) 261, 328, 973, 1066, 1332
Hpy8I GTNNAC 3 cut(s) 64, 221, 337
Hpy99I CGWCG 6 cut(s) 334, 368, 450, 514, 526, 1124
HpyAV CCTTC 4 cut(s) 47, 126, 733, 772
HpyCH4III ACNGT 2 cut(s) 334, 1019
HpyCH4IV ACGT 5 cut(s) 303, 372, 636, 936, 1202
HpyCH4V TGCA 5 cut(s) 83, 337, 483, 572, 804
HpyF10VI GCNNNNNNNGC 3 cut(s) 548, 928, 1174
HpyF3I CTNAG 4 cut(s) 15, 183, 224, 833
HpySE526I ACGT 5 cut(s) 303, 372, 636, 936, 1202
Hsp92I GRCGYC 4 cut(s) 179, 524, 1038, 1095
Hsp92II CATG 5 cut(s) 430, 440, 487, 576, 919
HspAI GCGC 4 cut(s) 256, 411, 1004, 1223
KpnI GGTACC 1 cut(s) 252
Ksp22I TGATCA 1 cut(s) 108
Kzo9I GATC 7 cut(s) 108, 135, 144, 235, 263, 889, 1116
LmnI GCTCC 2 cut(s) 161, 1024
Lsp1109I GCAGC 1 cut(s) 467
LweI GCATC 3 cut(s) 571, 973, 1114
MaeI CTAG 1 cut(s) 923
MaeII ACGT 5 cut(s) 303, 372, 636, 936, 1202
MaeIII GTNAC 6 cut(s) 467, 516, 529, 850, 937, 1243
MalI GATC 7 cut(s) 110, 137, 146, 237, 265, 891, 1118
MbiI CCGCTC 2 cut(s) 25, 1329
MboI GATC 7 cut(s) 108, 135, 144, 235, 263, 889, 1116
MboII GAAGA 8 cut(s) 98, 101, 104, 135, 182, 959, 1154, 1198
MhlI GDGCHC 2 cut(s) 339, 817
MluCI AATT 2 cut(s) 460, 1069
MlyI GAGTC 5 cut(s) 194, 500, 526, 894, 1240
MmeI TCCRAC 1 cut(s) 469
MroXI GAANNNNTTC 1 cut(s) 305
MseI TTAA 1 cut(s) 1181
MslI CAYNNNNRTG 4 cut(s) 47, 71, 332, 914
MspA1I CMGCKG 2 cut(s) 114, 1210
MspI CCGG 5 cut(s) 318, 399, 527, 539, 895
MspR9I CCNGG 2 cut(s) 243, 1164
MvaI CCWGG 2 cut(s) 243, 1164
MvnI CGCG 3 cut(s) 289, 413, 1006
MwoI GCNNNNNNNGC 3 cut(s) 548, 928, 1174
NdeII GATC 7 cut(s) 108, 135, 144, 235, 263, 889, 1116
NlaIII CATG 5 cut(s) 430, 440, 487, 576, 919
NlaIV GGNNCC 3 cut(s) 250, 543, 1276
NmeAIII GCCGAG 1 cut(s) 882
NmuCI GTSAC 6 cut(s) 467, 516, 529, 850, 937, 1243
NspI RCATGY 2 cut(s) 487, 576
OliI CACNNNNGTG 2 cut(s) 47, 332
PaqCI CACCTGC 1 cut(s) 68
PcsI WCGNNNNNNNCGW 4 cut(s) 369, 518, 1101, 1181
PdmI GAANNNNTTC 1 cut(s) 305
PfeI GAWTC 3 cut(s) 417, 557, 1149
PflFI GACNNNGTC 1 cut(s) 514
PkrI GCNGC 4 cut(s) 13, 178, 482, 868
Ple19I CGATCG 1 cut(s) 1119
PleI GAGTC 5 cut(s) 193, 500, 526, 894, 1240
PpsI GAGTC 5 cut(s) 193, 500, 526, 894, 1240
Ppu21I YACGTR 1 cut(s) 637
Psp6I CCWGG 2 cut(s) 241, 1162
PspFI CCCAGC 1 cut(s) 252
PspGI CCWGG 2 cut(s) 241, 1162
PspN4I GGNNCC 3 cut(s) 250, 543, 1276
PspPI GGNCC 1 cut(s) 819
PstNI CAGNNNCTG 1 cut(s) 597
PsyI GACNNNGTC 1 cut(s) 514
PvuI CGATCG 1 cut(s) 1119
PvuII CAGCTG 1 cut(s) 1210
RsaI GTAC 2 cut(s) 250, 632
RsaNI GTAC 2 cut(s) 249, 631
RseI CAYNNNNRTG 4 cut(s) 47, 71, 332, 914
Rsr2I CGGWCCG 1 cut(s) 819
RsrII CGGWCCG 1 cut(s) 819
SalI GTCGAC 1 cut(s) 62
SaqAI TTAA 1 cut(s) 1181
SatI GCNGC 4 cut(s) 12, 177, 481, 867
Sau3AI GATC 7 cut(s) 108, 135, 144, 235, 263, 889, 1116
Sau96I GGNCC 1 cut(s) 819
SchI GAGTC 5 cut(s) 194, 500, 526, 894, 1240
ScrFI CCNGG 2 cut(s) 243, 1164
SduI GDGCHC 2 cut(s) 339, 817
SfaNI GCATC 3 cut(s) 571, 973, 1114
SgrAI CRCCGGYG 1 cut(s) 526
SinI GGWCC 1 cut(s) 819
SmiMI CAYNNNNRTG 4 cut(s) 47, 71, 332, 914
SmlI CTYRAG 1 cut(s) 971
SmoI CTYRAG 1 cut(s) 971
Sse9I AATT 2 cut(s) 460, 1069
SsiI CCGC 9 cut(s) 25, 35, 71, 114, 159, 176, 287, 867, 1327
SspMI CTAG 1 cut(s) 923
StyD4I CCNGG 2 cut(s) 241, 1162
StyI CCWWGG 1 cut(s) 552
TaaI ACNGT 2 cut(s) 334, 1019
TaiI ACGT 5 cut(s) 306, 375, 639, 939, 1205
TaqII GACCGA 2 cut(s) 486, 836
TasI AATT 2 cut(s) 460, 1069
TauI GCSGC 2 cut(s) 179, 869
TfiI GAWTC 3 cut(s) 417, 557, 1149
Tru1I TTAA 1 cut(s) 1181
Tru9I TTAA 1 cut(s) 1181
TscAI CASTG 3 cut(s) 145, 204, 472
TseFI GTSAC 6 cut(s) 467, 516, 529, 850, 937, 1243
TseI GCWGC 2 cut(s) 11, 480
Tsp45I GTSAC 6 cut(s) 467, 516, 529, 850, 937, 1243
TspDTI ATGAA 4 cut(s) 443, 671, 932, 946
TspGWI ACGGA 1 cut(s) 550
TspRI CASTG 3 cut(s) 145, 204, 472
Tth111I GACNNNGTC 1 cut(s) 514
VneI GTGCAC 1 cut(s) 335
VpaK11BI GGWCC 1 cut(s) 819
XapI RAATTY 1 cut(s) 460
XceI RCATGY 2 cut(s) 487, 576
XcmI CCANNNNNNNNNTGG 2 cut(s) 376, 472
XmiI GTMKAC 1 cut(s) 63
XmnI GAANNNNTTC 1 cut(s) 305
XspI CTAG 1 cut(s) 923
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.