Rroxscaffold_2G00092550

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0002870
Physical Location & Seq
Reverse (-)
14113511 .. 14124953
11443 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00092550.1

Sequence Viewer

Length: 489 bp
ATGTGGACTAGGAAATACACACTCATACTATGTCAAAAGAATTTGTATCAGATTTTTGAAGACATTACTCATATCCTAACCTGGTTCTTTGGACAGTTCCGAGAGGACCGGATCTCAGACCGGTACATGAATAAGTTGAGGGAGGATTTTGGCTGCGTCCGGAGTCTTCCCGATAGTGTCAAGCTCACCGCCGCCGCTACGGCCGCGCCGCGGTGCAAGGCCACCACGTACGGGTGCGAGGTTTGTGGGAGCGTGGTTCCATTTCTTTCGGTGTGGGATGCTTGGGATCCCAATATCGCCCCAAGGGGTATCGTAACCCCTAGGCGGGAGCTTACCTCGTGGTGTATTGGCGATATGAGTCTTGAGCTTCGATACTTCTACTTAAGAGGTATGTGCATAATAACAAGTCCCCCAAGTCCCCGGTCAAGAGTTCTCTTGGGTGGGGAGTTTGACGATGGTAAAAGTATTGGAAGCTCTAGGCGAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

162

Amino Acids

18.57

Weight (kDa)

8.9

Isoelectric Point (pI)

52.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 206, 211
AccIII TCCGGA 1 cut(s) 159
AciI CCGC 7 cut(s) 189, 192, 195, 204, 209, 211, 325
AclWI GGATC 3 cut(s) 119, 281, 294
AcoI YGGCCR 1 cut(s) 201
AcsI RAATTY 1 cut(s) 40
AfaI GTAC 2 cut(s) 125, 230
AfiI CCNNNNNNNGG 4 cut(s) 210, 231, 324, 325
AflII CTTAAG 1 cut(s) 382
AgeI ACCGGT 1 cut(s) 120
AgsI TTSAA 1 cut(s) 59
AjnI CCWGG 1 cut(s) 80
AluBI AGCT 4 cut(s) 184, 331, 367, 474
AluI AGCT 4 cut(s) 184, 331, 367, 474
AlwI GGATC 3 cut(s) 119, 281, 294
Aor13HI TCCGGA 1 cut(s) 159
AoxI GGCC 2 cut(s) 201, 219
ApeKI GCWGC 1 cut(s) 153
ApoI RAATTY 1 cut(s) 40
AsiGI ACCGGT 1 cut(s) 120
AspA2I CCTAGG 1 cut(s) 320
AspLEI GCGC 1 cut(s) 208
AspS9I GGNCC 1 cut(s) 106
AsuC2I CCSGG 1 cut(s) 421
AsuHPI GGTGA 1 cut(s) 178
AvaII GGWCC 1 cut(s) 106
AvrII CCTAGG 1 cut(s) 320
BamHI GGATCC 1 cut(s) 286
BauI CACGAG 1 cut(s) 337
BbsI GAAGAC 2 cut(s) 66, 158
BbvI GCAGC 1 cut(s) 140
BccI CCATC 1 cut(s) 449
BceAI ACGGC 1 cut(s) 216
BciT130I CCWGG 1 cut(s) 82
BcnI CCSGG 1 cut(s) 421
BfaI CTAG 3 cut(s) 9, 321, 477
BfrI CTTAAG 1 cut(s) 382
BglI GCCNNNNNGGC 1 cut(s) 200
BisI GCNGC 5 cut(s) 154, 192, 195, 204, 209
BlnI CCTAGG 1 cut(s) 320
BlsI GCNGC 5 cut(s) 155, 193, 196, 205, 210
Bme1390I CCNGG 2 cut(s) 82, 421
Bme18I GGWCC 1 cut(s) 106
BmgT120I GGNCC 1 cut(s) 106
BmiI GGNNCC 2 cut(s) 258, 288
BmrFI CCNGG 2 cut(s) 82, 421
BmsI GCATC 1 cut(s) 268
BpiI GAAGAC 2 cut(s) 66, 158
BplI GAGNNNNNCTC 2 cut(s) 320, 352
BpuEI CTTGAG 1 cut(s) 383
BpuMI CCSGG 1 cut(s) 421
BsaAI YACGTR 1 cut(s) 228
BsaJI CCNNGG 4 cut(s) 209, 302, 320, 419
BsaWI WCCGGW 3 cut(s) 108, 120, 159
Bsc4I CCNNNNNNNGG 4 cut(s) 210, 231, 324, 325
Bse118I RCCGGY 1 cut(s) 120
BseAI TCCGGA 1 cut(s) 159
BseBI CCWGG 1 cut(s) 82
BseDI CCNNGG 4 cut(s) 209, 302, 320, 419
BseGI GGATG 1 cut(s) 283
BseLI CCNNNNNNNGG 4 cut(s) 210, 231, 324, 325
BseMII CTCAG 1 cut(s) 129
BseX3I CGGCCG 1 cut(s) 201
BseXI GCAGC 1 cut(s) 140
Bsh1236I CGCG 2 cut(s) 206, 211
Bsh1285I CGRYCG 1 cut(s) 204
BshFI GGCC 2 cut(s) 203, 221
BshTI ACCGGT 1 cut(s) 120
BsiEI CGRYCG 1 cut(s) 204
BsiSI CCGG 4 cut(s) 109, 121, 160, 421
BsiWI CGTACG 1 cut(s) 228
BslFI GGGAC 2 cut(s) 393, 402
BslI CCNNNNNNNGG 4 cut(s) 210, 231, 324, 325
BsmFI GGGAC 2 cut(s) 393, 402
BsnI GGCC 2 cut(s) 203, 221
Bsp13I TCCGGA 1 cut(s) 159
Bsp143I GATC 2 cut(s) 111, 286
BspACI CCGC 7 cut(s) 189, 192, 195, 204, 209, 211, 325
BspANI GGCC 2 cut(s) 203, 221
BspCNI CTCAG 1 cut(s) 128
BspEI TCCGGA 1 cut(s) 159
BspFNI CGCG 2 cut(s) 206, 211
BspLI GGNNCC 2 cut(s) 258, 288
BspPI GGATC 3 cut(s) 119, 281, 294
BspTI CTTAAG 1 cut(s) 382
BsrFI RCCGGY 1 cut(s) 120
BssAI RCCGGY 1 cut(s) 120
BssECI CCNNGG 4 cut(s) 209, 302, 320, 419
BssMI GATC 2 cut(s) 111, 286
BssSI CACGAG 1 cut(s) 337
BssT1I CCWWGG 2 cut(s) 302, 320
Bst2BI CACGAG 1 cut(s) 337
Bst2UI CCWGG 1 cut(s) 82
Bst4CI ACNGT 1 cut(s) 96
BstAFI CTTAAG 1 cut(s) 382
BstBAI YACGTR 1 cut(s) 228
BstDEI CTNAG 1 cut(s) 115
BstDSI CCRYGG 1 cut(s) 209
BstF5I GGATG 1 cut(s) 283
BstFNI CGCG 2 cut(s) 206, 211
BstHHI GCGC 1 cut(s) 208
BstKTI GATC 2 cut(s) 114, 289
BstMBI GATC 2 cut(s) 111, 286
BstMCI CGRYCG 1 cut(s) 204
BstMWI GCNNNNNNNGC 2 cut(s) 200, 203
BstNI CCWGG 1 cut(s) 82
BstSCI CCNGG 2 cut(s) 80, 419
BstUI CGCG 2 cut(s) 206, 211
BstV1I GCAGC 1 cut(s) 140
BstV2I GAAGAC 2 cut(s) 66, 158
BstX2I RGATCY 2 cut(s) 111, 286
BstYI RGATCY 2 cut(s) 111, 286
BstZI CGGCCG 1 cut(s) 201
BsuRI GGCC 2 cut(s) 203, 221
BtgI CCRYGG 1 cut(s) 209
BtsCI GGATG 1 cut(s) 283
CfoI GCGC 1 cut(s) 208
Cfr10I RCCGGY 1 cut(s) 120
Cfr13I GGNCC 1 cut(s) 106
Cfr42I CCGCGG 1 cut(s) 212
CseI GACGC 1 cut(s) 145
CsiI ACCWGGT 1 cut(s) 80
Csp6I GTAC 2 cut(s) 124, 229
CspAI ACCGGT 1 cut(s) 120
CviAII CATG 1 cut(s) 127
CviJI RGCY 7 cut(s) 153, 184, 203, 221, 331, 367, 474
CviKI_1 RGCY 7 cut(s) 153, 184, 203, 221, 331, 367, 474
CviQI GTAC 2 cut(s) 124, 229
DdeI CTNAG 1 cut(s) 115
DpnI GATC 2 cut(s) 113, 288
DpnII GATC 2 cut(s) 111, 286
EaeI YGGCCR 1 cut(s) 201
EagI CGGCCG 1 cut(s) 201
EclXI CGGCCG 1 cut(s) 201
Eco130I CCWWGG 2 cut(s) 302, 320
Eco47I GGWCC 1 cut(s) 106
Eco52I CGGCCG 1 cut(s) 201
EcoRII CCWGG 1 cut(s) 80
EcoT14I CCWWGG 2 cut(s) 302, 320
ErhI CCWWGG 2 cut(s) 302, 320
FaeI CATG 1 cut(s) 130
FaiI YATR 7 cut(s) 26, 31, 72, 128, 356, 392, 398
FaqI GGGAC 2 cut(s) 393, 402
FatI CATG 1 cut(s) 126
FauI CCCGC 1 cut(s) 318
Fnu4HI GCNGC 5 cut(s) 154, 192, 195, 204, 209
FokI GGATG 1 cut(s) 290
Fsp4HI GCNGC 5 cut(s) 154, 192, 195, 204, 209
FspBI CTAG 3 cut(s) 9, 321, 477
GlaI GCGC 1 cut(s) 207
GluI GCNGC 5 cut(s) 154, 192, 195, 204, 209
HaeIII GGCC 2 cut(s) 203, 221
HapII CCGG 4 cut(s) 109, 121, 160, 421
HgaI GACGC 1 cut(s) 145
HhaI GCGC 1 cut(s) 208
Hin1II CATG 1 cut(s) 130
Hin6I GCGC 1 cut(s) 206
HinP1I GCGC 1 cut(s) 206
HinfI GANTC 2 cut(s) 163, 358
HpaII CCGG 4 cut(s) 109, 121, 160, 421
HphI GGTGA 1 cut(s) 178
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 3 cut(s) 51, 101, 118
Hpy188III TCNNGA 4 cut(s) 160, 170, 362, 426
Hpy8I GTNNAC 1 cut(s) 6
HpyCH4III ACNGT 1 cut(s) 96
HpyCH4IV ACGT 1 cut(s) 227
HpyCH4V TGCA 2 cut(s) 216, 396
HpyF10VI GCNNNNNNNGC 2 cut(s) 200, 203
HpyF3I CTNAG 1 cut(s) 115
HpySE526I ACGT 1 cut(s) 227
Hsp92II CATG 1 cut(s) 130
HspAI GCGC 1 cut(s) 206
Kpn2I TCCGGA 1 cut(s) 159
KspI CCGCGG 1 cut(s) 212
Kzo9I GATC 2 cut(s) 111, 286
LmnI GCTCC 2 cut(s) 249, 328
LpnPI CCDG 6 cut(s) 67, 94, 122, 134, 173, 434
Lsp1109I GCAGC 1 cut(s) 140
LweI GCATC 1 cut(s) 268
MabI ACCWGGT 1 cut(s) 80
MaeI CTAG 3 cut(s) 9, 321, 477
MaeII ACGT 1 cut(s) 227
MaeIII GTNAC 1 cut(s) 313
MalI GATC 2 cut(s) 113, 288
MboI GATC 2 cut(s) 111, 286
MboII GAAGA 2 cut(s) 71, 158
MflI RGATCY 2 cut(s) 111, 286
MluCI AATT 1 cut(s) 40
MlyI GAGTC 2 cut(s) 172, 367
MnlI CCTC 6 cut(s) 97, 132, 136, 232, 346, 380
MroI TCCGGA 1 cut(s) 159
MseI TTAA 2 cut(s) 383, 487
MspA1I CMGCKG 1 cut(s) 211
MspCI CTTAAG 1 cut(s) 382
MspI CCGG 4 cut(s) 109, 121, 160, 421
MspR9I CCNGG 2 cut(s) 82, 421
MvaI CCWGG 1 cut(s) 82
MvnI CGCG 2 cut(s) 206, 211
MwoI GCNNNNNNNGC 2 cut(s) 200, 203
NciI CCSGG 1 cut(s) 421
NdeII GATC 2 cut(s) 111, 286
NlaIII CATG 1 cut(s) 130
NlaIV GGNNCC 2 cut(s) 258, 288
Pfl23II CGTACG 1 cut(s) 228
PinAI ACCGGT 1 cut(s) 120
PkrI GCNGC 5 cut(s) 155, 193, 196, 205, 210
PleI GAGTC 2 cut(s) 171, 366
PpsI GAGTC 2 cut(s) 171, 366
Ppu21I YACGTR 1 cut(s) 228
Psp6I CCWGG 1 cut(s) 80
PspGI CCWGG 1 cut(s) 80
PspLI CGTACG 1 cut(s) 228
PspN4I GGNNCC 2 cut(s) 258, 288
PspPI GGNCC 1 cut(s) 106
PsuI RGATCY 2 cut(s) 111, 286
RsaI GTAC 2 cut(s) 125, 230
RsaNI GTAC 2 cut(s) 124, 229
SacII CCGCGG 1 cut(s) 212
SaqAI TTAA 2 cut(s) 383, 487
SatI GCNGC 5 cut(s) 154, 192, 195, 204, 209
Sau3AI GATC 2 cut(s) 111, 286
Sau96I GGNCC 1 cut(s) 106
SchI GAGTC 2 cut(s) 172, 367
ScrFI CCNGG 2 cut(s) 82, 421
SetI ASST 9 cut(s) 83, 186, 230, 243, 333, 338, 369, 391, 476
SexAI ACCWGGT 1 cut(s) 80
SfaNI GCATC 1 cut(s) 268
Sfr303I CCGCGG 1 cut(s) 212
SgrBI CCGCGG 1 cut(s) 212
SinI GGWCC 1 cut(s) 106
SmlI CTYRAG 2 cut(s) 362, 382
SmoI CTYRAG 2 cut(s) 362, 382
Sse9I AATT 1 cut(s) 40
SsiI CCGC 7 cut(s) 189, 192, 195, 204, 209, 211, 325
SspMI CTAG 3 cut(s) 9, 321, 477
StyD4I CCNGG 2 cut(s) 80, 419
StyI CCWWGG 2 cut(s) 302, 320
TaaI ACNGT 1 cut(s) 96
TaiI ACGT 1 cut(s) 230
TaqI TCGA 1 cut(s) 370
TasI AATT 1 cut(s) 40
TauI GCSGC 4 cut(s) 194, 197, 206, 211
Tru1I TTAA 2 cut(s) 383, 487
Tru9I TTAA 2 cut(s) 383, 487
TseI GCWGC 1 cut(s) 153
TspDTI ATGAA 1 cut(s) 143
Vha464I CTTAAG 1 cut(s) 382
VpaK11BI GGWCC 1 cut(s) 106
XapI RAATTY 1 cut(s) 40
XmaJI CCTAGG 1 cut(s) 320
XspI CTAG 3 cut(s) 9, 321, 477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.