Rroxscaffold_4G00312850

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0002870
Physical Location & Seq
Forward (+)
36076075 .. 36078793
2719 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00312850.1

Sequence Viewer

Length: 756 bp
ATGGAGAAGGAAGGAAAGGGAGCAGCCCAAAGGGCTGCCTCTGTTTTGGTGGTCAGCTATTGTCGAAAGGAAGATGATAGTTCTCAAGGGTTAGTTCAATACATATGGAGTCGTGGTGGCTTGTGGCCAAAGAAAGAGGAAATGGGAATGCATCAGGGGCTTGGAGTTAAGGGAGAAGCATTCAACAGAGAGATGGCTCTAGGCTTGTTTTCTCTTCTTATTTTCCTGTTACTCTTAAAATCTGGGTTCTTGATGATGGGCGGCGATAGCGACGTTTTGAGTACGATGGCAAAATCTCAAGCTTGGATTGAGTCCGATGATGAAATCTCGTGCACACTCAAGTTCGGTGGCGAAGAGAGCCCAATTGAGAGCACGGTTCCCCCGCGCCACCGCGATGGTGTGTTGGTGCCGGTGGTTACGGCGGAGGCGGTGGCCCACGTTATCATCCTTGTGGGTGATGTGTGTGTCTTTGCCCTCCATGGTGGTGTTAAGGCGAGTGGTACCAACCTTCCTGCTCCTCGGCATCCCACCGGCAATGGTCGGTTGAATCTTCTCTCGGGTTTGGTTAGTGGCGTGCTCGCCTTGCGCCTCGGGATAGGATCCCGATATCACCCCGAAGGTATCGAAATCCCCACCGGGAGCTTACCTCGCCGGTGTATTGCAGATACGAGTCTTGTGCTTCCGATACTTGTGCTTAAGAGGTATGTGCATACCAACAAGTCCCCCAAGTCCCCGGTCAAGAGTTCTCTTGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

251

Amino Acids

26.82

Weight (kDa)

8.66

Isoelectric Point (pI)

47.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 500
AccB1I GGYRCC 2 cut(s) 406, 500
AccII CGCG 2 cut(s) 385, 393
AciI CCGC 5 cut(s) 261, 383, 391, 422, 428
AclWI GGATC 2 cut(s) 594, 607
AcoI YGGCCR 1 cut(s) 125
AfaI GTAC 2 cut(s) 283, 502
AflII CTTAAG 1 cut(s) 695
AgsI TTSAA 3 cut(s) 98, 184, 547
AluBI AGCT 3 cut(s) 57, 302, 642
AluI AGCT 3 cut(s) 57, 302, 642
Alw21I GWGCWC 3 cut(s) 335, 374, 579
Alw44I GTGCAC 1 cut(s) 331
AlwI GGATC 2 cut(s) 594, 607
Ama87I CYCGRG 2 cut(s) 556, 590
AoxI GGCC 2 cut(s) 125, 432
ApaLI GTGCAC 1 cut(s) 331
ApeKI GCWGC 2 cut(s) 23, 35
Asp718I GGTACC 1 cut(s) 500
AspLEI GCGC 2 cut(s) 387, 588
AspS9I GGNCC 1 cut(s) 433
AsuC2I CCSGG 2 cut(s) 637, 734
AsuHPI GGTGA 2 cut(s) 467, 602
AvaI CYCGRG 2 cut(s) 556, 590
BaeGI GKGCMC 1 cut(s) 335
BalI TGGCCA 1 cut(s) 127
BamHI GGATCC 1 cut(s) 599
BanI GGYRCC 2 cut(s) 406, 500
BanII GRGCYC 1 cut(s) 362
BauI CACGAG 1 cut(s) 328
Bbv12I GWGCWC 3 cut(s) 335, 374, 579
BbvI GCAGC 2 cut(s) 22, 35
BccI CCATC 4 cut(s) 187, 250, 280, 389
BceAI ACGGC 1 cut(s) 435
BcgI CGANNNNNNTGC 4 cut(s) 658, 673, 692, 707
BcnI CCSGG 2 cut(s) 637, 734
BfaI CTAG 1 cut(s) 200
BfrI CTTAAG 1 cut(s) 695
BglI GCCNNNNNGGC 1 cut(s) 32
BisI GCNGC 3 cut(s) 24, 36, 262
BlsI GCNGC 3 cut(s) 25, 37, 263
Bme1390I CCNGG 2 cut(s) 637, 734
BmeT110I CYCGRG 2 cut(s) 556, 590
BmgT120I GGNCC 1 cut(s) 433
BmiI GGNNCC 4 cut(s) 378, 408, 502, 601
BmrFI CCNGG 2 cut(s) 637, 734
BmsI GCATC 2 cut(s) 160, 532
BplI GAGNNNNNCTC 2 cut(s) 631, 663
BpuEI CTTGAG 3 cut(s) 69, 282, 323
BpuMI CCSGG 2 cut(s) 637, 734
BsaJI CCNNGG 4 cut(s) 478, 518, 589, 732
BsaXI ACNNNNNCTCC 2 cut(s) 100, 130
Bse118I RCCGGY 3 cut(s) 409, 530, 651
Bse3DI GCAATG 1 cut(s) 541
BseDI CCNNGG 4 cut(s) 478, 518, 589, 732
BseGI GGATG 2 cut(s) 444, 523
BseMI GCAATG 1 cut(s) 541
BseRI GAGGAG 1 cut(s) 507
BseSI GKGCMC 1 cut(s) 335
BseXI GCAGC 2 cut(s) 22, 35
Bsh1236I CGCG 2 cut(s) 385, 393
BshFI GGCC 2 cut(s) 127, 434
BshNI GGYRCC 2 cut(s) 406, 500
BsiHKAI GWGCWC 3 cut(s) 335, 374, 579
BsiHKCI CYCGRG 2 cut(s) 556, 590
BsiSI CCGG 5 cut(s) 410, 531, 636, 652, 734
BslFI GGGAC 2 cut(s) 706, 715
BsmFI GGGAC 2 cut(s) 706, 715
BsmI GAATGC 2 cut(s) 153, 179
BsnI GGCC 2 cut(s) 127, 434
BsoBI CYCGRG 2 cut(s) 556, 590
Bsp1286I GDGCHC 4 cut(s) 335, 362, 374, 579
Bsp143I GATC 1 cut(s) 599
Bsp19I CCATGG 1 cut(s) 478
BspACI CCGC 5 cut(s) 261, 383, 391, 422, 428
BspANI GGCC 2 cut(s) 127, 434
BspFNI CGCG 2 cut(s) 385, 393
BspLI GGNNCC 4 cut(s) 378, 408, 502, 601
BspPI GGATC 2 cut(s) 594, 607
BspT107I GGYRCC 2 cut(s) 406, 500
BspTI CTTAAG 1 cut(s) 695
BsrDI GCAATG 1 cut(s) 541
BsrFI RCCGGY 3 cut(s) 409, 530, 651
BssAI RCCGGY 3 cut(s) 409, 530, 651
BssECI CCNNGG 4 cut(s) 478, 518, 589, 732
BssMI GATC 1 cut(s) 599
BssSI CACGAG 1 cut(s) 328
BssT1I CCWWGG 1 cut(s) 478
Bst2BI CACGAG 1 cut(s) 328
Bst4CI ACNGT 1 cut(s) 376
Bst6I CTCTTC 2 cut(s) 219, 348
BstAFI CTTAAG 1 cut(s) 695
BstC8I GCNNGC 2 cut(s) 575, 579
BstDSI CCRYGG 1 cut(s) 478
BstF5I GGATG 2 cut(s) 444, 523
BstFNI CGCG 2 cut(s) 385, 393
BstHHI GCGC 2 cut(s) 387, 588
BstKTI GATC 1 cut(s) 602
BstMBI GATC 1 cut(s) 599
BstMWI GCNNNNNNNGC 6 cut(s) 32, 157, 267, 357, 583, 648
BstSCI CCNGG 2 cut(s) 635, 732
BstSLI GKGCMC 1 cut(s) 335
BstUI CGCG 2 cut(s) 385, 393
BstV1I GCAGC 2 cut(s) 22, 35
BstX2I RGATCY 1 cut(s) 599
BstXI CCANNNNNNTGG 1 cut(s) 395
BstYI RGATCY 1 cut(s) 599
BsuRI GGCC 2 cut(s) 127, 434
BtgI CCRYGG 1 cut(s) 478
BtgZI GCGATG 1 cut(s) 408
BtsCI GGATG 2 cut(s) 444, 523
Cac8I GCNNGC 2 cut(s) 575, 579
CfoI GCGC 2 cut(s) 387, 588
Cfr10I RCCGGY 3 cut(s) 409, 530, 651
Cfr13I GGNCC 1 cut(s) 433
Csp6I GTAC 2 cut(s) 282, 501
CspCI CAANNNNNGTGG 2 cut(s) 328, 363
CviAII CATG 1 cut(s) 479
CviQI GTAC 2 cut(s) 282, 501
DpnI GATC 1 cut(s) 601
DpnII GATC 1 cut(s) 599
EaeI YGGCCR 1 cut(s) 125
Eam1104I CTCTTC 2 cut(s) 219, 348
EarI CTCTTC 2 cut(s) 219, 348
EciI GGCGGA 1 cut(s) 437
Eco130I CCWWGG 1 cut(s) 478
Eco24I GRGCYC 1 cut(s) 362
Eco32I GATATC 1 cut(s) 608
Eco88I CYCGRG 2 cut(s) 556, 590
EcoRV GATATC 1 cut(s) 608
EcoT14I CCWWGG 1 cut(s) 478
EcoT22I ATGCAT 1 cut(s) 153
EcoT38I GRGCYC 1 cut(s) 362
ErhI CCWWGG 1 cut(s) 478
FaeI CATG 1 cut(s) 482
FaiI YATR 5 cut(s) 104, 106, 480, 705, 711
FaqI GGGAC 2 cut(s) 706, 715
FatI CATG 1 cut(s) 478
FauI CCCGC 1 cut(s) 390
FauNDI CATATG 1 cut(s) 104
Fnu4HI GCNGC 3 cut(s) 24, 36, 262
FokI GGATG 2 cut(s) 431, 510
FriOI GRGCYC 1 cut(s) 362
Fsp4HI GCNGC 3 cut(s) 24, 36, 262
FspBI CTAG 1 cut(s) 200
GlaI GCGC 2 cut(s) 386, 587
GluI GCNGC 3 cut(s) 24, 36, 262
HaeIII GGCC 2 cut(s) 127, 434
HapII CCGG 5 cut(s) 410, 531, 636, 652, 734
HhaI GCGC 2 cut(s) 387, 588
Hin1II CATG 1 cut(s) 482
Hin6I GCGC 2 cut(s) 385, 586
HinP1I GCGC 2 cut(s) 385, 586
HindIII AAGCTT 1 cut(s) 300
HinfI GANTC 4 cut(s) 109, 311, 547, 670
HpaII CCGG 5 cut(s) 410, 531, 636, 652, 734
HphI GGTGA 2 cut(s) 467, 602
Hpy166II GTNNAC 1 cut(s) 333
Hpy188I TCNGA 2 cut(s) 316, 684
Hpy188III TCNNGA 4 cut(s) 250, 592, 603, 739
Hpy8I GTNNAC 1 cut(s) 333
Hpy99I CGWCG 1 cut(s) 275
HpyAV CCTTC 3 cut(s) 5, 518, 611
HpyCH4III ACNGT 1 cut(s) 376
HpyCH4IV ACGT 2 cut(s) 273, 438
HpyCH4V TGCA 4 cut(s) 151, 333, 662, 709
HpyF10VI GCNNNNNNNGC 6 cut(s) 32, 157, 267, 357, 583, 648
HpySE526I ACGT 2 cut(s) 273, 438
Hsp92II CATG 1 cut(s) 482
HspAI GCGC 2 cut(s) 385, 586
KpnI GGTACC 1 cut(s) 504
Kzo9I GATC 1 cut(s) 599
LmnI GCTCC 3 cut(s) 20, 520, 639
LpnPI CCDG 9 cut(s) 140, 228, 239, 423, 525, 544, 649, 665, 747
Lsp1109I GCAGC 2 cut(s) 22, 35
LweI GCATC 2 cut(s) 160, 532
MaeI CTAG 1 cut(s) 200
MaeII ACGT 2 cut(s) 273, 438
MaeIII GTNAC 2 cut(s) 228, 415
MalI GATC 1 cut(s) 601
MboI GATC 1 cut(s) 599
MboII GAAGA 4 cut(s) 83, 206, 365, 542
MfeI CAATTG 1 cut(s) 363
MflI RGATCY 1 cut(s) 599
MhlI GDGCHC 4 cut(s) 335, 362, 374, 579
MlsI TGGCCA 1 cut(s) 127
MluCI AATT 1 cut(s) 363
MluNI TGGCCA 1 cut(s) 127
MlyI GAGTC 3 cut(s) 118, 320, 679
MnlI CCTC 8 cut(s) 49, 130, 418, 485, 528, 599, 657, 693
Mox20I TGGCCA 1 cut(s) 127
Mph1103I ATGCAT 1 cut(s) 153
MscI TGGCCA 1 cut(s) 127
MseI TTAA 4 cut(s) 168, 236, 489, 696
MslI CAYNNNNRTG 3 cut(s) 393, 449, 483
Msp20I TGGCCA 1 cut(s) 127
MspCI CTTAAG 1 cut(s) 695
MspI CCGG 5 cut(s) 410, 531, 636, 652, 734
MspR9I CCNGG 2 cut(s) 637, 734
MunI CAATTG 1 cut(s) 363
Mva1269I GAATGC 2 cut(s) 153, 179
MvnI CGCG 2 cut(s) 385, 393
MwoI GCNNNNNNNGC 6 cut(s) 32, 157, 267, 357, 583, 648
NciI CCSGG 2 cut(s) 637, 734
NcoI CCATGG 1 cut(s) 478
NdeI CATATG 1 cut(s) 104
NdeII GATC 1 cut(s) 599
NlaIII CATG 1 cut(s) 482
NlaIV GGNNCC 4 cut(s) 378, 408, 502, 601
NmeAIII GCCGAG 1 cut(s) 499
NsiI ATGCAT 1 cut(s) 153
PctI GAATGC 2 cut(s) 153, 179
PfeI GAWTC 1 cut(s) 547
PkrI GCNGC 3 cut(s) 25, 37, 263
PleI GAGTC 3 cut(s) 117, 319, 678
PpsI GAGTC 3 cut(s) 117, 319, 678
PspN4I GGNNCC 4 cut(s) 378, 408, 502, 601
PspPI GGNCC 1 cut(s) 433
PsuI RGATCY 1 cut(s) 599
RsaI GTAC 2 cut(s) 283, 502
RsaNI GTAC 2 cut(s) 282, 501
RseI CAYNNNNRTG 3 cut(s) 393, 449, 483
SaqAI TTAA 4 cut(s) 168, 236, 489, 696
SatI GCNGC 3 cut(s) 24, 36, 262
Sau3AI GATC 1 cut(s) 599
Sau96I GGNCC 1 cut(s) 433
SchI GAGTC 3 cut(s) 118, 320, 679
ScrFI CCNGG 2 cut(s) 637, 734
SduI GDGCHC 4 cut(s) 335, 362, 374, 579
SetI ASST 9 cut(s) 59, 276, 304, 441, 510, 622, 644, 649, 704
SfaNI GCATC 2 cut(s) 160, 532
SgrAI CRCCGGYG 1 cut(s) 651
SmiMI CAYNNNNRTG 3 cut(s) 393, 449, 483
SmlI CTYRAG 4 cut(s) 84, 297, 338, 695
SmoI CTYRAG 4 cut(s) 84, 297, 338, 695
Sse9I AATT 1 cut(s) 363
SsiI CCGC 5 cut(s) 261, 383, 391, 422, 428
SspMI CTAG 1 cut(s) 200
StyD4I CCNGG 2 cut(s) 635, 732
StyI CCWWGG 1 cut(s) 478
TaaI ACNGT 1 cut(s) 376
TaiI ACGT 2 cut(s) 276, 441
TaqI TCGA 2 cut(s) 64, 624
TasI AATT 1 cut(s) 363
TauI GCSGC 1 cut(s) 264
TfiI GAWTC 1 cut(s) 547
Tru1I TTAA 4 cut(s) 168, 236, 489, 696
Tru9I TTAA 4 cut(s) 168, 236, 489, 696
TseI GCWGC 2 cut(s) 23, 35
TspDTI ATGAA 1 cut(s) 336
Vha464I CTTAAG 1 cut(s) 695
VneI GTGCAC 1 cut(s) 331
XspI CTAG 1 cut(s) 200
Zsp2I ATGCAT 1 cut(s) 153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.