Rroxscaffold_5G00353510

Belongs to the UDP-glycosyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001515
Physical Location & Seq
Forward (+)
31375714 .. 31377327
1614 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00353510.1

Sequence Viewer

Length: 1614 bp
ATGCCAACCCAAACAGAAGACGTCATTGATGAGCATATACTCAGAAACTCTCACAAGCAATCCAAAAGCATATATAGTCAGTCCATTCATATAGTAATCCCAAAACCCATTCAGAGTCAAAAGCAAAAAAATCAAAGAATTTCAGAGATGGGTTTTGATAAGGAACACATAGTGATGCTACCATTCTTGGCTCAAGGCCATCTGATTCCATTCCTAGCCCTAGCCAAGCAAATCCAGCACAGAACAGGGTTCACCATCACCATCGCCACCACCGCCCTCAACGCCCATTACCTTCGGTCTGCTCAACCCAACACCAACATCCACTTGGCCGAGCTTCCCTTCCGCAGCGAAGACTATGGCCTCCCGCCCAACACAGAGAACACAGAGAACATACCTCTCAACGTAGTCGGAAACCTCTTCACTGCATCGCAACATCTCGAAGCTCCGACTCGCCGCCTTGTCTCGGACATCACCAAAAAAGATGGCAAGCCGCCGCTTTGCTTAATCTCTGACGTGTTTTTTGGGTGGGCAGTGAATGTAGCGAAAAGCTTCGGCACCGTCAACGTCGCCTTCACCACCGGTGGGGCTTACGGCACGGCGGCGTACGTATCCATCTGGCAACATCTCCCGCATCGTAGTGTGTCTTCAGAAGACGAGGAGTTCCACCTTCCGGGGTTCCCGGAACGGTGTCGTTTGACACCGTCTCACTTGCATCCGTTTTTGAGAGCCGCCGACGGTACTGATTCTTGGTCGAGATATTTCCAGCTGCAGATCTCGCTCTCGATGAAGTCTTTTGGTTGCATATGCAACACTATTGAGGAAATCGAGCCGTTCGGATTGCAAGTGTTGCGAAATTACCTTAAGGTTCCTGTTTGGTCCGTTTTGGCTTCCAATATTTCCAGCCAAAAATATTCCGGCAAGAAATCCGGAATTTCGGCTGAGAAATGTATTGAGTGGCTCGACTCGCACGCTTCCGATTCGGTGGTGTACATCTCGTTCGGTTCCCAGAACACGATCAGTGCCTCCCAGATGATAGAATTGGCCAAAGGATTGGAAAAGAGCGGGAAGCCTTTTGTCTGGGTCATAAGGCCGCCGGTGGGTCACGACTTGAAGGGCGAGTTCCGACCGGAGTGGCTGCCGGAGCGTTTCGAGGAGCGAATGAGGGAGACGAAGCAGGGGTTATTGGTGCACAACTGGGCCTCGCAGCTGGAGATTCTGTCGCACGCGGCGACGCGAGCGTTTGTGAGTCACTGCGGGTGGAACTCGGTGTTGGAGAGCTTGAGCAGAGGGGTGCCGATCATCGGGTGGCCGCTGGCGGCGGAGCAGGCGTATAACTCGAAGATGTTGGTGGAGGAGATGGGTGTGAGTGTGGAGCTGACGAGGGGTGTGAAGAGTAATGTGGAAGCGGAGGAGGTGAGGAGAGTGATTGAGTTGGTGGCGGACGAGAGTGGGGAAGGTGGTGAGATGAGGAGAAGAGCTGGTGAGATTATGGAGAAGATAAGAGGAGCTGTAGGGGAGGATGGTGAAGCAAAGGGGTCTTCCCTTAAAGCTATAGATGATTTTGTTTCTGAACTTCTTTCACAGAGAGAAGGAAGAGGATCAAAAATCCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0008194 GO:0016740 GO:0016757 GO:0016758 GO:0035251 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0046527 GO:0080043 GO:0080044
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UDPGT

Protein Analysis

537

Amino Acids

59.88

Weight (kDa)

6.46

Isoelectric Point (pI)

53.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_N PF26168 57 - 184 2.1e-06 Glycosyltransferase, N-terminal domain
UDPGT PF00201 316 - 460 2.7e-21 UDP-glucoronosyl and UDP-glucosyl transferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 24
AccB1I GGYRCC 2 cut(s) 554, 1291
AccBSI CCGCTC 1 cut(s) 1062
AccII CGCG 2 cut(s) 1226, 1234
AccIII TCCGGA 1 cut(s) 926
AclWI GGATC 1 cut(s) 1606
AcoI YGGCCR 3 cut(s) 327, 1041, 1307
AcsI RAATTY 2 cut(s) 138, 930
AcuI CTGAAG 1 cut(s) 630
AcyI GRCGYC 1 cut(s) 21
AdeI CACNNNGTG 1 cut(s) 172
AfaI GTAC 3 cut(s) 605, 739, 989
AfiI CCNNNNNNNGG 5 cut(s) 463, 582, 670, 1097, 1301
AflII CTTAAG 1 cut(s) 860
AflIII ACRYGT 1 cut(s) 513
AgeI ACCGGT 1 cut(s) 578
AgsI TTSAA 1 cut(s) 1111
AjiI CACGTC 1 cut(s) 514
AjuI GAANNNNNNNTTGG 2 cut(s) 1253, 1285
Alw21I GWGCWC 1 cut(s) 1191
Alw26I GTCTC 3 cut(s) 466, 708, 1160
Alw44I GTGCAC 1 cut(s) 1187
AlwI GGATC 1 cut(s) 1606
Aor13HI TCCGGA 1 cut(s) 926
AoxI GGCC 7 cut(s) 196, 327, 358, 1041, 1088, 1197, 1307
ApaLI GTGCAC 1 cut(s) 1187
ApeKI GCWGC 4 cut(s) 345, 766, 1135, 1204
ApoI RAATTY 2 cut(s) 138, 930
ArsI GACNNNNNNTTYG 2 cut(s) 503, 535
AsiGI ACCGGT 1 cut(s) 578
Asp700I GAANNNNTTC 1 cut(s) 548
AspS9I GGNCC 2 cut(s) 876, 1197
AsuC2I CCSGG 2 cut(s) 672, 680
AsuHPI GGTGA 8 cut(s) 244, 250, 463, 565, 1426, 1472, 1493, 1535
AvaII GGWCC 1 cut(s) 876
BaeGI GKGCMC 1 cut(s) 1191
BalI TGGCCA 1 cut(s) 1043
BanI GGYRCC 2 cut(s) 554, 1291
BarI GAAGNNNNNNTAC 2 cut(s) 628, 660
BbsI GAAGAC 5 cut(s) 24, 357, 636, 657, 1530
Bbv12I GWGCWC 1 cut(s) 1191
BbvI GCAGC 4 cut(s) 357, 753, 1122, 1216
BccI CCATC 8 cut(s) 142, 207, 263, 269, 476, 620, 1351, 1514
BceAI ACGGC 3 cut(s) 607, 612, 814
BciVI GTATCC 1 cut(s) 619
BcnI CCSGG 2 cut(s) 672, 680
BcoDI GTCTC 3 cut(s) 466, 708, 1160
BfaI CTAG 2 cut(s) 215, 221
BfmI CTRYAG 3 cut(s) 767, 1509, 1551
BfrI CTTAAG 1 cut(s) 860
BfuI GTATCC 1 cut(s) 619
BglII AGATCT 1 cut(s) 771
Bme1390I CCNGG 2 cut(s) 672, 680
Bme18I GGWCC 1 cut(s) 876
BmgBI CACGTC 1 cut(s) 514
BmgT120I GGNCC 2 cut(s) 876, 1197
BmiI GGNNCC 5 cut(s) 556, 677, 867, 1003, 1293
BmrFI CCNGG 2 cut(s) 672, 680
BmrI ACTGGG 1 cut(s) 1204
BmsI GCATC 4 cut(s) 165, 434, 640, 721
BmuI ACTGGG 1 cut(s) 1204
BpiI GAAGAC 5 cut(s) 24, 357, 636, 657, 1530
BpmI CTGGAG 1 cut(s) 1229
BpuEI CTTGAG 2 cut(s) 177, 1300
BpuMI CCSGG 2 cut(s) 672, 680
BsaAI YACGTR 1 cut(s) 607
BsaHI GRCGYC 1 cut(s) 21
BsaJI CCNNGG 1 cut(s) 671
BsaWI WCCGGW 3 cut(s) 578, 926, 1126
BsaXI ACNNNNNCTCC 4 cut(s) 1202, 1232, 1313, 1343
Bsc4I CCNNNNNNNGG 5 cut(s) 463, 582, 670, 1097, 1301
Bse118I RCCGGY 2 cut(s) 578, 1093
Bse1I ACTGG 2 cut(s) 1199, 1609
BseAI TCCGGA 1 cut(s) 926
BseDI CCNNGG 1 cut(s) 671
BseGI GGATG 3 cut(s) 318, 712, 1525
BseLI CCNNNNNNNGG 5 cut(s) 463, 582, 670, 1097, 1301
BseMII CTCAG 2 cut(s) 55, 930
BseNI ACTGG 2 cut(s) 1199, 1609
BseRI GAGGAG 7 cut(s) 671, 1166, 1367, 1424, 1432, 1483, 1518
BseSI GKGCMC 1 cut(s) 1191
BseXI GCAGC 4 cut(s) 357, 753, 1122, 1216
Bsh1236I CGCG 2 cut(s) 1226, 1234
Bsh1285I CGRYCG 1 cut(s) 1127
BshFI GGCC 7 cut(s) 198, 329, 360, 1043, 1090, 1199, 1309
BshNI GGYRCC 2 cut(s) 554, 1291
BshTI ACCGGT 1 cut(s) 578
BsiEI CGRYCG 1 cut(s) 1127
BsiHKAI GWGCWC 1 cut(s) 1191
BsiSI CCGG 8 cut(s) 579, 671, 680, 915, 927, 1094, 1127, 1139
BsiWI CGTACG 1 cut(s) 603
BslI CCNNNNNNNGG 5 cut(s) 463, 582, 670, 1097, 1301
BsmAI GTCTC 3 cut(s) 466, 708, 1160
BsmBI CGTCTC 2 cut(s) 708, 1160
BsnI GGCC 7 cut(s) 198, 329, 360, 1043, 1090, 1199, 1309
Bsp1286I GDGCHC 1 cut(s) 1191
Bsp13I TCCGGA 1 cut(s) 926
Bsp1407I TGTACA 1 cut(s) 987
Bsp143I GATC 4 cut(s) 771, 1014, 1296, 1598
BspANI GGCC 7 cut(s) 198, 329, 360, 1043, 1090, 1199, 1309
BspCNI CTCAG 2 cut(s) 54, 931
BspEI TCCGGA 1 cut(s) 926
BspFNI CGCG 2 cut(s) 1226, 1234
BspLI GGNNCC 5 cut(s) 556, 677, 867, 1003, 1293
BspMAI CTGCAG 1 cut(s) 771
BspPI GGATC 1 cut(s) 1606
BspQI GCTCTTC 1 cut(s) 1468
BspT107I GGYRCC 2 cut(s) 554, 1291
BspTI CTTAAG 1 cut(s) 860
BsrBI CCGCTC 1 cut(s) 1062
BsrFI RCCGGY 2 cut(s) 578, 1093
BsrGI TGTACA 1 cut(s) 987
BsrI ACTGG 2 cut(s) 1199, 1609
BssAI RCCGGY 2 cut(s) 578, 1093
BssECI CCNNGG 1 cut(s) 671
BssMI GATC 4 cut(s) 771, 1014, 1296, 1598
BssNI GRCGYC 1 cut(s) 21
Bst4CI ACNGT 4 cut(s) 559, 687, 702, 737
Bst6I CTCTTC 4 cut(s) 422, 1385, 1468, 1588
BstACI GRCGYC 1 cut(s) 21
BstAFI CTTAAG 1 cut(s) 860
BstAPI GCANNNNNTGC 1 cut(s) 847
BstAUI TGTACA 1 cut(s) 987
BstBAI YACGTR 1 cut(s) 607
BstC8I GCNNGC 6 cut(s) 488, 969, 1224, 1236, 1314, 1326
BstDEI CTNAG 2 cut(s) 41, 939
BstF5I GGATG 3 cut(s) 318, 712, 1525
BstFNI CGCG 2 cut(s) 1226, 1234
BstKTI GATC 4 cut(s) 774, 1017, 1299, 1601
BstMAI GTCTC 3 cut(s) 466, 708, 1160
BstMBI GATC 4 cut(s) 771, 1014, 1296, 1598
BstMCI CGRYCG 1 cut(s) 1127
BstMWI GCNNNNNNNGC 9 cut(s) 235, 272, 281, 775, 847, 964, 1141, 1235, 1325
BstSCI CCNGG 2 cut(s) 670, 678
BstSFI CTRYAG 3 cut(s) 767, 1509, 1551
BstSLI GKGCMC 1 cut(s) 1191
BstSNI TACGTA 1 cut(s) 607
BstUI CGCG 2 cut(s) 1226, 1234
BstV1I GCAGC 4 cut(s) 357, 753, 1122, 1216
BstV2I GAAGAC 5 cut(s) 24, 357, 636, 657, 1530
BstX2I RGATCY 1 cut(s) 771
BstXI CCANNNNNNTGG 1 cut(s) 1051
BstYI RGATCY 1 cut(s) 771
BsuI GTATCC 1 cut(s) 619
BsuRI GGCC 7 cut(s) 198, 329, 360, 1043, 1090, 1199, 1309
BtgZI GCGATG 2 cut(s) 247, 411
BtrI CACGTC 1 cut(s) 514
BtsCI GGATG 3 cut(s) 318, 712, 1525
BtsI GCAGTG 3 cut(s) 420, 537, 1249
BtsIMutI CAGTG 4 cut(s) 420, 537, 1024, 1249
Cac8I GCNNGC 6 cut(s) 488, 969, 1224, 1236, 1314, 1326
Cfr10I RCCGGY 2 cut(s) 578, 1093
Cfr13I GGNCC 2 cut(s) 876, 1197
CseI GACGC 1 cut(s) 1240
Csp6I GTAC 3 cut(s) 604, 738, 988
CspAI ACCGGT 1 cut(s) 578
CviQI GTAC 3 cut(s) 604, 738, 988
DdeI CTNAG 2 cut(s) 41, 939
DpnI GATC 4 cut(s) 773, 1016, 1298, 1600
DpnII GATC 4 cut(s) 771, 1014, 1296, 1598
DraIII CACNNNGTG 1 cut(s) 172
EaeI YGGCCR 3 cut(s) 327, 1041, 1307
Eam1104I CTCTTC 4 cut(s) 422, 1385, 1468, 1588
EarI CTCTTC 4 cut(s) 422, 1385, 1468, 1588
EciI GGCGGA 2 cut(s) 1334, 1454
Eco105I TACGTA 1 cut(s) 607
Eco47I GGWCC 1 cut(s) 876
Eco57I CTGAAG 1 cut(s) 630
Esp3I CGTCTC 2 cut(s) 708, 1160
FauI CCCGC 4 cut(s) 372, 636, 1055, 1247
FauNDI CATATG 1 cut(s) 803
FokI GGATG 3 cut(s) 305, 699, 1532
FspBI CTAG 2 cut(s) 215, 221
GsuI CTGGAG 1 cut(s) 1229
HaeIII GGCC 7 cut(s) 198, 329, 360, 1043, 1090, 1199, 1309
HapII CCGG 8 cut(s) 579, 671, 680, 915, 927, 1094, 1127, 1139
HgaI GACGC 1 cut(s) 1240
Hin1I GRCGYC 1 cut(s) 21
HincII GTYRAC 1 cut(s) 562
HindII GTYRAC 1 cut(s) 562
HindIII AAGCTT 1 cut(s) 547
HinfI GANTC 8 cut(s) 115, 205, 448, 743, 962, 977, 1213, 1246
HpaII CCGG 8 cut(s) 579, 671, 680, 915, 927, 1094, 1127, 1139
HphI GGTGA 8 cut(s) 244, 250, 463, 565, 1426, 1472, 1493, 1535
Hpy166II GTNNAC 4 cut(s) 252, 562, 988, 1189
Hpy188III TCNNGA 5 cut(s) 437, 753, 781, 927, 1103
Hpy8I GTNNAC 4 cut(s) 252, 562, 988, 1189
Hpy99I CGWCG 3 cut(s) 569, 737, 1234
HpyAV CCTTC 7 cut(s) 302, 349, 580, 677, 1105, 1448, 1583
HpyCH4III ACNGT 4 cut(s) 559, 687, 702, 737
HpyCH4IV ACGT 5 cut(s) 21, 402, 513, 564, 606
HpyCH4V TGCA 7 cut(s) 425, 712, 769, 801, 807, 841, 1189
HpyF10VI GCNNNNNNNGC 9 cut(s) 235, 272, 281, 775, 847, 964, 1141, 1235, 1325
HpyF3I CTNAG 2 cut(s) 41, 939
HpySE526I ACGT 5 cut(s) 21, 402, 513, 564, 606
Hsp92I GRCGYC 1 cut(s) 21
Kpn2I TCCGGA 1 cut(s) 926
Kzo9I GATC 4 cut(s) 771, 1014, 1296, 1598
LguI GCTCTTC 1 cut(s) 1468
LmnI GCTCC 6 cut(s) 448, 1141, 1153, 1321, 1372, 1505
Lsp1109I GCAGC 4 cut(s) 357, 753, 1122, 1216
LweI GCATC 4 cut(s) 165, 434, 640, 721
MaeI CTAG 2 cut(s) 215, 221
MaeII ACGT 5 cut(s) 21, 402, 513, 564, 606
MaeIII GTNAC 2 cut(s) 1100, 1247
MalI GATC 4 cut(s) 773, 1016, 1298, 1600
MbiI CCGCTC 1 cut(s) 1062
MboI GATC 4 cut(s) 771, 1014, 1296, 1598
MflI RGATCY 1 cut(s) 771
MhlI GDGCHC 1 cut(s) 1191
MlsI TGGCCA 1 cut(s) 1043
MluCI AATT 4 cut(s) 138, 853, 930, 1037
MluNI TGGCCA 1 cut(s) 1043
MlyI GAGTC 4 cut(s) 124, 442, 956, 1255
MmeI TCCRAC 4 cut(s) 388, 470, 1147, 1251
Mox20I TGGCCA 1 cut(s) 1043
MroI TCCGGA 1 cut(s) 926
MroXI GAANNNNTTC 1 cut(s) 548
MscI TGGCCA 1 cut(s) 1043
MseI TTAA 3 cut(s) 503, 861, 1545
MslI CAYNNNNRTG 2 cut(s) 173, 636
Msp20I TGGCCA 1 cut(s) 1043
MspA1I CMGCKG 3 cut(s) 766, 1207, 1312
MspCI CTTAAG 1 cut(s) 860
MspI CCGG 8 cut(s) 579, 671, 680, 915, 927, 1094, 1127, 1139
MspR9I CCNGG 2 cut(s) 672, 680
MvnI CGCG 2 cut(s) 1226, 1234
MwoI GCNNNNNNNGC 9 cut(s) 235, 272, 281, 775, 847, 964, 1141, 1235, 1325
NciI CCSGG 2 cut(s) 672, 680
NdeI CATATG 1 cut(s) 803
NdeII GATC 4 cut(s) 771, 1014, 1296, 1598
NlaIV GGNNCC 5 cut(s) 556, 677, 867, 1003, 1293
NmeAIII GCCGAG 1 cut(s) 355
NmuCI GTSAC 2 cut(s) 1100, 1247
PciSI GCTCTTC 1 cut(s) 1468
PcsI WCGNNNNNNNCGW 1 cut(s) 1226
PdmI GAANNNNTTC 1 cut(s) 548
PfeI GAWTC 4 cut(s) 205, 743, 977, 1213
Pfl23II CGTACG 1 cut(s) 603
PflFI GACNNNGTC 1 cut(s) 700
PfoI TCCNGGA 1 cut(s) 678
PinAI ACCGGT 1 cut(s) 578
PleI GAGTC 4 cut(s) 123, 442, 956, 1254
PpsI GAGTC 4 cut(s) 123, 442, 956, 1254
Ppu21I YACGTR 1 cut(s) 607
PspLI CGTACG 1 cut(s) 603
PspN4I GGNNCC 5 cut(s) 556, 677, 867, 1003, 1293
PspPI GGNCC 2 cut(s) 876, 1197
PsrI GAACNNNNNNTAC 2 cut(s) 980, 1012
PstI CTGCAG 1 cut(s) 771
PsuI RGATCY 1 cut(s) 771
PsyI GACNNNGTC 1 cut(s) 700
PvuII CAGCTG 2 cut(s) 766, 1207
RsaI GTAC 3 cut(s) 605, 739, 989
RsaNI GTAC 3 cut(s) 604, 738, 988
RseI CAYNNNNRTG 2 cut(s) 173, 636
SapI GCTCTTC 1 cut(s) 1468
SaqAI TTAA 3 cut(s) 503, 861, 1545
Sau3AI GATC 4 cut(s) 771, 1014, 1296, 1598
Sau96I GGNCC 2 cut(s) 876, 1197
SchI GAGTC 4 cut(s) 124, 442, 956, 1255
ScrFI CCNGG 2 cut(s) 672, 680
SduI GDGCHC 1 cut(s) 1191
SfaNI GCATC 4 cut(s) 165, 434, 640, 721
SfcI CTRYAG 3 cut(s) 767, 1509, 1551
SgrAI CRCCGGYG 2 cut(s) 578, 1093
SinI GGWCC 1 cut(s) 876
SmiMI CAYNNNNRTG 2 cut(s) 173, 636
SmlI CTYRAG 3 cut(s) 192, 860, 1279
SmoI CTYRAG 3 cut(s) 192, 860, 1279
SnaBI TACGTA 1 cut(s) 607
Sse9I AATT 4 cut(s) 138, 853, 930, 1037
SspI AATATT 2 cut(s) 895, 911
SspMI CTAG 2 cut(s) 215, 221
StyD4I CCNGG 2 cut(s) 670, 678
TaaI ACNGT 4 cut(s) 559, 687, 702, 737
TaiI ACGT 5 cut(s) 24, 405, 516, 567, 609
TaqI TCGA 7 cut(s) 438, 752, 782, 825, 960, 1149, 1337
TaqII GACCGA 1 cut(s) 285
TasI AATT 4 cut(s) 138, 853, 930, 1037
TatI WGTACW 1 cut(s) 987
TauI GCSGC 9 cut(s) 456, 493, 496, 602, 731, 1093, 1229, 1312, 1319
TfiI GAWTC 4 cut(s) 205, 743, 977, 1213
Tru1I TTAA 3 cut(s) 503, 861, 1545
Tru9I TTAA 3 cut(s) 503, 861, 1545
TscAI CASTG 4 cut(s) 427, 537, 1024, 1256
TseFI GTSAC 2 cut(s) 1100, 1247
TseI GCWGC 4 cut(s) 345, 766, 1135, 1204
Tsp45I GTSAC 2 cut(s) 1100, 1247
TspDTI ATGAA 2 cut(s) 77, 800
TspGWI ACGGA 2 cut(s) 705, 868
TspRI CASTG 4 cut(s) 427, 537, 1024, 1256
Tth111I GACNNNGTC 1 cut(s) 700
Vha464I CTTAAG 1 cut(s) 860
VneI GTGCAC 1 cut(s) 1187
VpaK11BI GGWCC 1 cut(s) 876
XapI RAATTY 2 cut(s) 138, 930
XcmI CCANNNNNNNNNTGG 1 cut(s) 322
XmnI GAANNNNTTC 1 cut(s) 548
XspI CTAG 2 cut(s) 215, 221
ZraI GACGTC 1 cut(s) 22
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.