Rorug05G0238200

Aluminum-activated malate transporter 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000374
Physical Location & Seq
Reverse (-)
25099799 .. 25131527
31729 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0238200.1

Sequence Viewer

Length: 3024 bp
ATGAAGATGGCAAGTAGAGATAAGTTGATCATGATGCTCATGATATTGGTTTTCTGCTTCATATGCATAAACTCAGTCAAAGTTGAAGCACAGTCTGCAACTCTTATTGCTTCAGAAGTGGAAGCTCTGAAAGAAATAGCTAAACAAATAGGAAAGAATAATTGGAACTTTAGTGTCGACCCTTGCAGCAATGACAAGAGCTGGGTCACTCCAATACCTCCTCGTCAGTTCAAAAACAACGTCACCTGCAGCTGCTCCTTCTCTGATGGTGCATGCCACGTCACCACCATCTTTCTTAAGGGGCAAGACCTTGCTGGTGTGCTTCCACCGTCTATTGCAAAGTTACCCTACCTGACAAATGTCAATTTCATGAGGAACTACCTCAATGGCAGCATACCGCGAGAATGGGCGTCAACGAAGTTGCAATATCTGTCCATTGCTGTGAACAACTTATCAGGACCAATCCCGAGCTACCTGGGAAACATTACCACTCTAACATATCTGAACATAGACAATAACATGTTCTCAGGAACTGTTCCTCCTGAGCTTGGAAACTTGGTTAACTTGCAGAATCTTATTCTTAGTGCAAACAATCTCACAGGAGAACTGCCGGTGGCTCTTATGAATCTGACTAAGTTAACAGAACTGAGGATTAGCAGCAACAACTTCACTGGAAGAATGCCCGAGTTTCTTCAAAGTTGGAATCAACTTCAGAAATTAGGGATCCAAGCTAGTGGTCTACAAGGCCCCATTCCATCGAGCATTTCTGCCTTGAGTAATTTAACAGAACTAAGGATCAGCGACTTAAATGGAGGGGGTTCAGTATTTCGATACTTGAGCCATATGATAAGCTTGCAAAGATTAATGCTCAGGAGCTGTCACTTATCTGGACCTTTTCCTTCTTATTTATCAGCACTGTCACAGTTGAATACATTAGATCTAAGCTTCAACAGATTGGAGGGCAGCATTCCTGATTTGGCAGATCTGCCAGACTTGCAGTACTTGTATCTAACAAGCAACTTGCTTACTGGATCTATTCCAGACTGGATCAAGAGCAGAGATAATCTCTACGTGATAGATGTTTCTTACAATGATTTTTCTGAGAGCTCTGAGTCACCTTATTGTACAGAAAACCTGAATGTGTTCAAAAGCTTTTCTGCACGTGATAACTCATTATATGCAGAGTGCCTGAAGAGCTCTCCATGTCCAAAAGATCAGTACTCATTGCATATCAATTGTGGTGGTAAAGCAACCACCATTGGAGGAATCAACTTCGAAGCGGATCAAGACCCAGGAGGTGCAGCACATTTCGTTCCTTCAGGGCCTAGCTGGGGATTCAGTAGCACTGGACATTTCTGGGATACTAGCGTCACCTCTAATGACTATATAGCAAATAATATTTCTATACTCGAAATGGACAACTCTGAGTTGTACACAAATGCACGCATCTCTCCTCTTTCTCTTACGTATTATGGACGCTGCTTCGGCAGTGGAAATTATACTGTGAAGCTTTACTTTTCAGAGATTATAATCAGAGGCAATAGATCTTTTTCGAGTCTTGGAAGACGGATCTTCGATGTTTATATTCAGGAGAAGCTAGTGTTGAAGGATTTCAACATTGAAAAGGCTGCCCAAGGGGTTGATAAGCCAGTCATCAGGGAATTTAAAGCAGTTGAGGTTAAGAACAAAACTTTAGAGATCCGATTTCATTGGTCTGGGAAGGGGACAACAACTTCCCCGAATAGAGGAGTACATGGTCCCCTTATATCAGCTATCTCTGTAGAGTTTGAGTCTGATGATGATAGCAAAAAGAAGGTAATTGTGGTTGCAGCTTCAGCTGGAGGTTCGCTTTTGTGTCTTGCTTTCTTAATCTTAGGCATTCTTTGGTGGAAAGGCTGTCTGGATAGCATGACATCAAGGGGAAAAGCTCATAGAGGACTGGATCTGCAAACGGGTTTTTTCACCTTCAAGCAAATTCAAGCTGCCACTAACAACTTTGCTCTGGAAAACAAAATTGGGGAAGGTGGTTTTGGGTCTGTCTACAAGGGTGTATTATTGGATGGTACAATAGTTGCGGTTAAACAACTATCTTCAAAATCAAAGCAGGGAAATCGTGAATTTGTGAATGAAATAGGCATGATTTCTAGTTTGCAACATCCAAATCTTGTTAGATTGTATGGATCCTGTATTGAAGCAAATGAACTACTGTTGGTTTATGAATACATGGAAAACAATAGCCTTGCACATGCTTTGTTTGGTCCAGAGGAAGGTCCGGTGAGATTGGACTGGCCTACAAGGCAGAGAATATGTATAGGCATAGCAAAAGGTCTGGCTTTTTTGCATGAGGAATCAGCACTTAAGGTTGTACATAGGGACATCAAAACAACGAATGTGCTATTGGACCAAGACCTTAATGCTAAGATCTCGGATTTTGGTCTGGCCAAGCTTGATGAAGAAGAGAACACCCACATTAGCACCAGAGTTGCTGGAACTATAGGATACATGGCGCCAGAATATGCACTATGGGGTTATTTAACCTTCAAAGCAGATGTCTTCAGTTTTGGTGTTGTTGCATTAGAAATAGTTGCTGGAAAAAACAACATGAAATTTCGACCAAATGAGAATTTTGTAGGCCTTGTGGATTGGGCTCTTGTTTTACAACAAAAAGAGAGTTTAGTGGAACTGGTGGATCCAAGGTTAGGATTGGATTTCAGTAAGGAAGAGGCAGTTAGAATGGTCAAAGTAGCTCTGCTATGCATCAATCCGGCAGCTGCACTTAGGCCTAACATGTCTGTAGTCGTGAGTATGCTTGAAGGGCGAGCTCCTGTCGATGAATTGATTATGGATCCAAGTATATATAGTGAAGAAATGAGGTTAACAGCATTGAGAAATCCGTTTGAGCAGATTGCAGAAGAGAGTGCAGGTGGAACTAAGAGCTTGATACATTCATCAGATGCATCATGGAATGGTTCTTCTGCTCCTACAACATCTTCAGATCTCTATAAAATCAGTCCTAGTTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ALMT

Protein Analysis

1007

Amino Acids

110.9

Weight (kDa)

5.87

Isoelectric Point (pI)

37.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 141 - 340 2.1e-14 Leucine-rich repeat region
LRR_8 PF13855 284 - 342 1.2e-07 Leucine rich repeat
LRR_4 PF12799 308 - 344 5.8e-06 Leucine Rich repeats (2 copies)
Malectin PF11721 408 - 592 2.8e-36 Malectin domain
Pkinase PF00069 667 - 869 6.1e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 668 - 935 1.6e-46 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000374)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G08430 AT1G08440 AT2G27240 AT2G27240 AT4G17585
fragaria_vesca FvH4_3g26440 FvH4_3g26440 FvH4_3g26490 FvH4_3g26520 FvH4_3g26530 FvH4_6g51850 FvH4_6g51860
malus_domestica MD00G1017600.v1.1 MD00G1049200.v1.1 MD00G1049700.v1.1 MD03G1155200.v1.1 MD03G1155400.v1.1 MD03G1155500.v1.1 MD11G1173000.v1.1
prunus_persica Prupe.6G144000_v2.0.a1 Prupe.6G144100_v2.0.a1 Prupe.6G144200_v2.0.a1
pyrus_communis pycom03g10930 pycom11g14730
rosa_chinensis RchiOBHm_Chr5g0048251 RchiOBHm_Chr5g0048321 RchiOBHm_Chr5g0048411 RchiOBHm_Chr5g0048431 RchiOBHm_Chr5g0048441 RchiOBHm_Chr5g0048461 RchiOBHm_Chr5g0048471 RchiOBHm_Chr5g0048481
rosa_laevigata RLG00000034506 RLG00000034507 RLG00000034512 RLG00000034514 RLG00000034515 RLG00000034517 RLG00000034519 RLG00000034523 RLG00000034525
rosa_multiflora Rmu_sc0000398.1_g000002 Rmu_sc0001240.1_g000004 Rmu_sc0001240.1_g000016 Rmu_sc0002563.1_g000001 Rmu_sc0002563.1_g000017 Rmu_sc0002563.1_g000037 Rmu_sc0002563.1_g000048 Rmu_sc0002563.1_g000055 Rmu_sc0002563.1_g000073 Rmu_sc0002591.1_g000001 Rmu_sc0002591.1_g000037 Rmu_sc0002591.1_g000045
rosa_roxburghii Rroxscaffold_1G00033370 Rroxscaffold_1G00033390 Rroxscaffold_1G00033420 Rroxscaffold_1G00033440 Rroxscaffold_1G00033450 Rroxscaffold_1G00033530 Rroxscaffold_1G00033600
rosa_rugosa Rorug05G0237500 Rorug05G0237700 Rorug05G0237800 Rorug05G0238000 Rorug05G0238200 Rorug05G0238300
rosa_samantha Rh5AG316700 Rh5AG316900 Rh5AG317100 Rh5AG317400 Rh5AG317500 Rh5AG317800 Rh5AG318200 Rh5AG318500 Rh5BG327700 Rh5BG327800 Rh5BG327900 Rh5BG328100 Rh5BG328200 Rh5BG328500 Rh5BG328600 Rh5CG353400 Rh5CG353500 Rh5CG353600 Rh5CG353800 Rh5CG354100 Rh5CG354300 Rh5CG354600 Rh5DG338400 Rh5DG338900 Rh5DG339000 Rh5DG339200 Rh5DG339300 Rh5DG339400 Rh5DG339600 Rh5DG339700
rosa_wichuraiana Rw5G029670 Rw5G029690 Rw5G029700 Rw5G029720 Rw5G029730 Rw5G029750 Rw5G029760 Rw5G029770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1529
AarI CACCTGC 2 cut(s) 254, 2912
Acc36I ACCTGC 2 cut(s) 254, 2912
AccB1I GGYRCC 1 cut(s) 2506
AccI GTMKAC 3 cut(s) 177, 739, 2040
AccII CGCG 1 cut(s) 400
AciI CCGC 3 cut(s) 398, 1280, 2075
AcoI YGGCCR 1 cut(s) 2439
AcsI RAATTY 5 cut(s) 1661, 1974, 2117, 2606, 2623
AcuI CTGAAG 7 cut(s) 96, 695, 1211, 1302, 1818, 2539, 2976
AcvI CACGTG 1 cut(s) 1163
AcyI GRCGYC 2 cut(s) 410, 2507
AfaI GTAC 7 cut(s) 1001, 1126, 1220, 1433, 1752, 2065, 2367
AfiI CCNNNNNNNGG 5 cut(s) 548, 1331, 1745, 2267, 2699
AflII CTTAAG 2 cut(s) 296, 2357
AflIII ACRYGT 2 cut(s) 519, 2787
AjiI CACGTC 1 cut(s) 280
AjnI CCWGG 2 cut(s) 474, 1291
AjuI GAANNNNNNNTTGG 8 cut(s) 145, 177, 1998, 2013, 2030, 2045, 2381, 2413
AloI GAACNNNNNNTCC 2 cut(s) 523, 555
Alw21I GWGCWC 3 cut(s) 1109, 1199, 2824
AlwNI CAGNNNCTG 2 cut(s) 533, 876
Ama87I CYCGRG 2 cut(s) 466, 683
AoxI GGCC 6 cut(s) 745, 1322, 2288, 2439, 2632, 2780
ApoI RAATTY 5 cut(s) 1661, 1974, 2117, 2606, 2623
ArsI GACNNNNNNTTYG 4 cut(s) 225, 257, 2535, 2567
AseI ATTAAT 1 cut(s) 863
Asp700I GAANNNNTTC 2 cut(s) 1142, 1610
AspLEI GCGC 1 cut(s) 2509
AspS9I GGNCC 8 cut(s) 458, 746, 890, 1322, 1757, 2258, 2270, 2401
AsuHPI GGTGA 6 cut(s) 235, 274, 1107, 1363, 1954, 2287
AsuII TTCGAA 1 cut(s) 1275
AvaI CYCGRG 2 cut(s) 466, 683
AvaII GGWCC 6 cut(s) 458, 890, 1757, 2258, 2270, 2401
BaeI ACNNNNGTAYC 4 cut(s) 1353, 1386, 2055, 2088
BalI TGGCCA 1 cut(s) 2441
BamHI GGATCC 4 cut(s) 723, 2180, 2689, 2845
BanI GGYRCC 1 cut(s) 2506
BanII GRGCYC 4 cut(s) 1109, 1199, 2650, 2824
BbrPI CACGTG 1 cut(s) 1163
BbsI GAAGAC 2 cut(s) 1570, 2545
Bbv12I GWGCWC 3 cut(s) 1109, 1199, 2824
BccI CCATC 4 cut(s) 260, 296, 763, 2054
BcgI CGANNNNNNTGC 2 cut(s) 2093, 2127
BciT130I CCWGG 2 cut(s) 476, 1293
BciVI GTATCC 2 cut(s) 1354, 2492
BclI TGATCA 1 cut(s) 27
BfaI CTAG 6 cut(s) 732, 1326, 1365, 1598, 2145, 3015
BfmI CTRYAG 4 cut(s) 247, 1779, 2493, 2793
BfoI RGCGCY 1 cut(s) 2510
BfrI CTTAAG 2 cut(s) 296, 2357
BfuAI ACCTGC 2 cut(s) 254, 2912
BfuI GTATCC 2 cut(s) 1354, 2492
BglI GCCNNNNNGGC 1 cut(s) 2296
BglII AGATCT 5 cut(s) 937, 982, 1544, 2421, 2995
BmcAI AGTACT 2 cut(s) 1001, 1220
Bme1390I CCNGG 2 cut(s) 476, 1293
Bme18I GGWCC 6 cut(s) 458, 890, 1757, 2258, 2270, 2401
BmeT110I CYCGRG 2 cut(s) 466, 683
BmgBI CACGTC 1 cut(s) 280
BmgT120I GGNCC 8 cut(s) 458, 746, 890, 1322, 1757, 2258, 2270, 2401
BmiI GGNNCC 7 cut(s) 725, 748, 1759, 2182, 2508, 2691, 2847
BmrFI CCNGG 2 cut(s) 476, 1293
BmsI GCATC 5 cut(s) 24, 1455, 2766, 2944, 2966
BoxI GACNNNNGTC 1 cut(s) 359
BpiI GAAGAC 2 cut(s) 1570, 2545
BplI GAGNNNNNCTC 2 cut(s) 1050, 1082
BpmI CTGGAG 1 cut(s) 1860
Bpu10I CCTNAGC 2 cut(s) 543, 869
Bpu14I TTCGAA 1 cut(s) 1275
BpuEI CTTGAG 2 cut(s) 793, 856
BsaAI YACGTR 3 cut(s) 1072, 1163, 1467
BsaBI GATNNNNATC 1 cut(s) 1529
BsaHI GRCGYC 2 cut(s) 410, 2507
BsaJI CCNNGG 4 cut(s) 475, 1291, 1633, 2693
BsaWI WCCGGW 1 cut(s) 2272
BsaXI ACNNNNNCTCC 2 cut(s) 523, 553
Bsc4I CCNNNNNNNGG 5 cut(s) 548, 1331, 1745, 2267, 2699
Bse118I RCCGGY 1 cut(s) 610
Bse1I ACTGG 8 cut(s) 676, 1033, 1049, 1351, 1649, 1944, 2291, 2688
Bse3DI GCAATG 3 cut(s) 196, 435, 1223
Bse8I GATNNNNATC 1 cut(s) 1529
BseBI CCWGG 2 cut(s) 476, 1293
BseDI CCNNGG 4 cut(s) 475, 1291, 1633, 2693
BseGI GGATG 2 cut(s) 2065, 2155
BseJI GATNNNNATC 1 cut(s) 1529
BseLI CCNNNNNNNGG 5 cut(s) 548, 1331, 1745, 2267, 2699
BseMI GCAATG 3 cut(s) 196, 435, 1223
BseMII CTCAG 8 cut(s) 87, 534, 540, 638, 883, 1092, 1101, 1416
BseNI ACTGG 8 cut(s) 676, 1033, 1049, 1351, 1649, 1944, 2291, 2688
BseRI GAGGAG 3 cut(s) 210, 1443, 1761
BseYI CCCAGC 2 cut(s) 201, 1329
BsgI GTGCAG 4 cut(s) 1143, 1320, 2757, 2940
Bsh1236I CGCG 1 cut(s) 400
BshFI GGCC 6 cut(s) 747, 1324, 2290, 2441, 2634, 2782
BshNI GGYRCC 1 cut(s) 2506
BsiHKAI GWGCWC 3 cut(s) 1109, 1199, 2824
BsiHKCI CYCGRG 2 cut(s) 466, 683
BsiSI CCGG 3 cut(s) 611, 2273, 2765
BslFI GGGAC 3 cut(s) 1738, 1743, 2387
BslI CCNNNNNNNGG 5 cut(s) 548, 1331, 1745, 2267, 2699
BsmFI GGGAC 3 cut(s) 1738, 1743, 2387
BsmI GAATGC 3 cut(s) 684, 966, 1878
BsnI GGCC 6 cut(s) 747, 1324, 2290, 2441, 2634, 2782
BsoBI CYCGRG 2 cut(s) 466, 683
Bsp119I TTCGAA 1 cut(s) 1275
Bsp1286I GDGCHC 4 cut(s) 1109, 1199, 2650, 2824
Bsp1407I TGTACA 3 cut(s) 1124, 1431, 2365
BspACI CCGC 3 cut(s) 398, 1280, 2075
BspANI GGCC 6 cut(s) 747, 1324, 2290, 2441, 2634, 2782
BspCNI CTCAG 8 cut(s) 86, 535, 539, 639, 882, 1093, 1102, 1417
BspFNI CGCG 1 cut(s) 400
BspHI TCATGA 3 cut(s) 30, 39, 369
BspLI GGNNCC 7 cut(s) 725, 748, 1759, 2182, 2508, 2691, 2847
BspMAI CTGCAG 1 cut(s) 251
BspMI ACCTGC 2 cut(s) 254, 2912
BspQI GCTCTTC 1 cut(s) 1187
BspT104I TTCGAA 1 cut(s) 1275
BspT107I GGYRCC 1 cut(s) 2506
BspTI CTTAAG 2 cut(s) 296, 2357
BsrDI GCAATG 3 cut(s) 196, 435, 1223
BsrFI RCCGGY 1 cut(s) 610
BsrGI TGTACA 3 cut(s) 1124, 1431, 2365
BsrI ACTGG 8 cut(s) 676, 1033, 1049, 1351, 1649, 1944, 2291, 2688
BssAI RCCGGY 1 cut(s) 610
BssECI CCNNGG 4 cut(s) 475, 1291, 1633, 2693
BssNI GRCGYC 2 cut(s) 410, 2507
BssT1I CCWWGG 2 cut(s) 1633, 2693
Bst2UI CCWGG 2 cut(s) 476, 1293
Bst4CI ACNGT 7 cut(s) 93, 330, 535, 918, 924, 1504, 2208
Bst6I CTCTTC 4 cut(s) 1187, 2451, 2715, 2907
BstACI GRCGYC 2 cut(s) 410, 2507
BstAFI CTTAAG 2 cut(s) 296, 2357
BstAPI GCANNNNNTGC 1 cut(s) 95
BstAUI TGTACA 3 cut(s) 1124, 1431, 2365
BstBAI YACGTR 3 cut(s) 1072, 1163, 1467
BstBI TTCGAA 1 cut(s) 1275
BstC8I GCNNGC 4 cut(s) 274, 854, 1444, 2820
BstF5I GGATG 2 cut(s) 2065, 2155
BstFNI CGCG 1 cut(s) 400
BstH2I RGCGCY 1 cut(s) 2510
BstHHI GCGC 1 cut(s) 2509
BstMWI GCNNNNNNNGC 8 cut(s) 63, 95, 994, 1194, 1485, 1835, 2296, 2815
BstNI CCWGG 2 cut(s) 476, 1293
BstNSI RCATGY 4 cut(s) 276, 523, 2249, 2791
BstPAI GACNNNNGTC 1 cut(s) 359
BstSCI CCNGG 2 cut(s) 474, 1291
BstSFI CTRYAG 4 cut(s) 247, 1779, 2493, 2793
BstSNI TACGTA 1 cut(s) 1467
BstUI CGCG 1 cut(s) 400
BstV2I GAAGAC 2 cut(s) 1570, 2545
BstXI CCANNNNNNTGG 1 cut(s) 734
BsuI GTATCC 2 cut(s) 1354, 2492
BsuRI GGCC 6 cut(s) 747, 1324, 2290, 2441, 2634, 2782
BtrI CACGTC 1 cut(s) 280
BtsCI GGATG 2 cut(s) 2065, 2155
BtsI GCAGTG 1 cut(s) 1495
BtsIMutI CAGTG 4 cut(s) 669, 914, 1344, 1495
BveI ACCTGC 2 cut(s) 254, 2912
Cac8I GCNNGC 4 cut(s) 274, 854, 1444, 2820
CaiI CAGNNNCTG 2 cut(s) 533, 876
CciI TCATGA 3 cut(s) 30, 39, 369
CfoI GCGC 1 cut(s) 2509
Cfr10I RCCGGY 1 cut(s) 610
Cfr13I GGNCC 8 cut(s) 458, 746, 890, 1322, 1757, 2258, 2270, 2401
CseI GACGC 3 cut(s) 399, 1357, 1485
Csp6I GTAC 7 cut(s) 1000, 1125, 1219, 1432, 1751, 2064, 2366
CspCI CAANNNNNGTGG 2 cut(s) 1222, 1257
CviQI GTAC 7 cut(s) 1000, 1125, 1219, 1432, 1751, 2064, 2366
DinI GGCGCC 1 cut(s) 2508
DraI TTTAAA 1 cut(s) 1666
EaeI YGGCCR 1 cut(s) 2439
Eam1104I CTCTTC 4 cut(s) 1187, 2451, 2715, 2907
EarI CTCTTC 4 cut(s) 1187, 2451, 2715, 2907
Ecl136II GAGCTC 3 cut(s) 1107, 1197, 2822
Eco105I TACGTA 1 cut(s) 1467
Eco130I CCWWGG 2 cut(s) 1633, 2693
Eco147I AGGCCT 2 cut(s) 2634, 2782
Eco24I GRGCYC 4 cut(s) 1109, 1199, 2650, 2824
Eco47I GGWCC 6 cut(s) 458, 890, 1757, 2258, 2270, 2401
Eco53kI GAGCTC 3 cut(s) 1107, 1197, 2822
Eco57I CTGAAG 7 cut(s) 96, 695, 1211, 1302, 1818, 2539, 2976
Eco72I CACGTG 1 cut(s) 1163
Eco88I CYCGRG 2 cut(s) 466, 683
EcoICRI GAGCTC 3 cut(s) 1107, 1197, 2822
EcoO109I RGGNCCY 2 cut(s) 746, 1322
EcoRII CCWGG 2 cut(s) 474, 1291
EcoT14I CCWWGG 2 cut(s) 1633, 2693
EcoT22I ATGCAT 3 cut(s) 68, 2759, 2959
EcoT38I GRGCYC 4 cut(s) 1109, 1199, 2650, 2824
EgeI GGCGCC 1 cut(s) 2508
EheI GGCGCC 1 cut(s) 2508
ErhI CCWWGG 2 cut(s) 1633, 2693
FalI AAGNNNNNCTT 2 cut(s) 1499, 1531
FaqI GGGAC 3 cut(s) 1738, 1743, 2387
FauNDI CATATG 2 cut(s) 62, 843
FbaI TGATCA 1 cut(s) 27
FblI GTMKAC 3 cut(s) 177, 739, 2040
FokI GGATG 2 cut(s) 2072, 2142
FriOI GRGCYC 4 cut(s) 1109, 1199, 2650, 2824
FspBI CTAG 6 cut(s) 732, 1326, 1365, 1598, 2145, 3015
GlaI GCGC 1 cut(s) 2508
GsaI CCCAGC 2 cut(s) 205, 1333
GsuI CTGGAG 1 cut(s) 1860
HaeII RGCGCY 1 cut(s) 2510
HaeIII GGCC 6 cut(s) 747, 1324, 2290, 2441, 2634, 2782
HapII CCGG 3 cut(s) 611, 2273, 2765
HgaI GACGC 3 cut(s) 399, 1357, 1485
HhaI GCGC 1 cut(s) 2509
Hin1I GRCGYC 2 cut(s) 410, 2507
Hin6I GCGC 1 cut(s) 2507
HinP1I GCGC 1 cut(s) 2507
HincII GTYRAC 5 cut(s) 178, 414, 562, 639, 2877
HindII GTYRAC 5 cut(s) 178, 414, 562, 639, 2877
HindIII AAGCTT 5 cut(s) 850, 943, 1150, 1508, 2444
HinfI GANTC 9 cut(s) 571, 625, 703, 1112, 1266, 1335, 1555, 1790, 2348
HpaI GTTAAC 3 cut(s) 562, 639, 2877
HpaII CCGG 3 cut(s) 611, 2273, 2765
HphI GGTGA 6 cut(s) 235, 274, 1107, 1363, 1954, 2287
Hpy166II GTNNAC 9 cut(s) 178, 414, 445, 562, 639, 740, 1434, 2041, 2877
Hpy8I GTNNAC 9 cut(s) 178, 414, 445, 562, 639, 740, 1434, 2041, 2877
HpyCH4III ACNGT 7 cut(s) 93, 330, 535, 918, 924, 1504, 2208
HpyCH4IV ACGT 5 cut(s) 240, 279, 1071, 1162, 1466
HpyF10VI GCNNNNNNNGC 8 cut(s) 63, 95, 994, 1194, 1485, 1835, 2296, 2815
HpySE526I ACGT 5 cut(s) 240, 279, 1071, 1162, 1466
Hsp92I GRCGYC 2 cut(s) 410, 2507
HspAI GCGC 1 cut(s) 2507
KasI GGCGCC 1 cut(s) 2506
Ksp22I TGATCA 1 cut(s) 27
KspAI GTTAAC 3 cut(s) 562, 639, 2877
LguI GCTCTTC 1 cut(s) 1187
LmnI GCTCC 4 cut(s) 260, 873, 2827, 2983
LweI GCATC 5 cut(s) 24, 1455, 2766, 2944, 2966
MaeI CTAG 6 cut(s) 732, 1326, 1365, 1598, 2145, 3015
MaeII ACGT 5 cut(s) 240, 279, 1071, 1162, 1466
MaeIII GTNAC 8 cut(s) 205, 241, 280, 342, 878, 918, 1113, 1369
MfeI CAATTG 1 cut(s) 1234
MhlI GDGCHC 4 cut(s) 1109, 1199, 2650, 2824
MlsI TGGCCA 1 cut(s) 2441
MluNI TGGCCA 1 cut(s) 2441
Mly113I GGCGCC 1 cut(s) 2507
MlyI GAGTC 3 cut(s) 1121, 1564, 1799
MmeI TCCRAC 1 cut(s) 680
Mox20I TGGCCA 1 cut(s) 2441
Mph1103I ATGCAT 3 cut(s) 68, 2759, 2959
MroXI GAANNNNTTC 2 cut(s) 1142, 1610
MscI TGGCCA 1 cut(s) 2441
MslI CAYNNNNRTG 1 cut(s) 440
Msp20I TGGCCA 1 cut(s) 2441
MspA1I CMGCKG 3 cut(s) 252, 1838, 2771
MspCI CTTAAG 2 cut(s) 296, 2357
MspI CCGG 3 cut(s) 611, 2273, 2765
MspR9I CCNGG 2 cut(s) 476, 1293
MunI CAATTG 1 cut(s) 1234
Mva1269I GAATGC 3 cut(s) 684, 966, 1878
MvaI CCWGG 2 cut(s) 476, 1293
MvnI CGCG 1 cut(s) 400
MwoI GCNNNNNNNGC 8 cut(s) 63, 95, 994, 1194, 1485, 1835, 2296, 2815
NarI GGCGCC 1 cut(s) 2507
NdeI CATATG 2 cut(s) 62, 843
NlaIV GGNNCC 7 cut(s) 725, 748, 1759, 2182, 2508, 2691, 2847
NmuCI GTSAC 7 cut(s) 205, 241, 280, 878, 918, 1113, 1369
NsiI ATGCAT 3 cut(s) 68, 2759, 2959
NspI RCATGY 4 cut(s) 276, 523, 2249, 2791
NspV TTCGAA 1 cut(s) 1275
PaeI GCATGC 1 cut(s) 276
PagI TCATGA 3 cut(s) 30, 39, 369
PaqCI CACCTGC 2 cut(s) 254, 2912
PceI AGGCCT 2 cut(s) 2634, 2782
PciI ACATGT 2 cut(s) 519, 2787
PciSI GCTCTTC 1 cut(s) 1187
PctI GAATGC 3 cut(s) 684, 966, 1878
PdmI GAANNNNTTC 2 cut(s) 1142, 1610
PfeI GAWTC 6 cut(s) 571, 625, 703, 1266, 1335, 2348
PleI GAGTC 3 cut(s) 1120, 1563, 1798
PluTI GGCGCC 1 cut(s) 2510
PmaCI CACGTG 1 cut(s) 1163
PmlI CACGTG 1 cut(s) 1163
PpsI GAGTC 3 cut(s) 1120, 1563, 1798
Ppu21I YACGTR 3 cut(s) 1072, 1163, 1467
PscI ACATGT 2 cut(s) 519, 2787
PshAI GACNNNNGTC 1 cut(s) 359
PshBI ATTAAT 1 cut(s) 863
PsiI TTATAA 1 cut(s) 1529
Psp124BI GAGCTC 3 cut(s) 1109, 1199, 2824
Psp6I CCWGG 2 cut(s) 474, 1291
PspCI CACGTG 1 cut(s) 1163
PspFI CCCAGC 2 cut(s) 201, 1329
PspGI CCWGG 2 cut(s) 474, 1291
PspN4I GGNNCC 7 cut(s) 725, 748, 1759, 2182, 2508, 2691, 2847
PspPI GGNCC 8 cut(s) 458, 746, 890, 1322, 1757, 2258, 2270, 2401
PstI CTGCAG 1 cut(s) 251
PstNI CAGNNNCTG 2 cut(s) 533, 876
PvuII CAGCTG 3 cut(s) 252, 1838, 2771
RsaI GTAC 7 cut(s) 1001, 1126, 1220, 1433, 1752, 2065, 2367
RsaNI GTAC 7 cut(s) 1000, 1125, 1219, 1432, 1751, 2064, 2366
RseI CAYNNNNRTG 1 cut(s) 440
SacI GAGCTC 3 cut(s) 1109, 1199, 2824
SalI GTCGAC 1 cut(s) 176
SapI GCTCTTC 1 cut(s) 1187
Sau96I GGNCC 8 cut(s) 458, 746, 890, 1322, 1757, 2258, 2270, 2401
ScaI AGTACT 2 cut(s) 1001, 1220
SchI GAGTC 3 cut(s) 1121, 1564, 1799
ScrFI CCNGG 2 cut(s) 476, 1293
SduI GDGCHC 4 cut(s) 1109, 1199, 2650, 2824
SfaNI GCATC 5 cut(s) 24, 1455, 2766, 2944, 2966
SfcI CTRYAG 4 cut(s) 247, 1779, 2493, 2793
SfoI GGCGCC 1 cut(s) 2508
SfuI TTCGAA 1 cut(s) 1275
SinI GGWCC 6 cut(s) 458, 890, 1757, 2258, 2270, 2401
SmiMI CAYNNNNRTG 1 cut(s) 440
SmlI CTYRAG 4 cut(s) 296, 772, 835, 2357
SmoI CTYRAG 4 cut(s) 296, 772, 835, 2357
SnaBI TACGTA 1 cut(s) 1467
SphI GCATGC 1 cut(s) 276
SseBI AGGCCT 2 cut(s) 2634, 2782
SsiI CCGC 3 cut(s) 398, 1280, 2075
SspDI GGCGCC 1 cut(s) 2506
SspI AATATT 1 cut(s) 1399
SspMI CTAG 6 cut(s) 732, 1326, 1365, 1598, 2145, 3015
SstI GAGCTC 3 cut(s) 1109, 1199, 2824
StuI AGGCCT 2 cut(s) 2634, 2782
StyD4I CCNGG 2 cut(s) 474, 1291
StyI CCWWGG 2 cut(s) 1633, 2693
TaaI ACNGT 7 cut(s) 93, 330, 535, 918, 924, 1504, 2208
TaiI ACGT 5 cut(s) 243, 282, 1074, 1165, 1469
TaqI TCGA 9 cut(s) 177, 758, 829, 1275, 1410, 1553, 1575, 2611, 2829
TatI WGTACW 6 cut(s) 999, 1124, 1218, 1431, 1750, 2365
TfiI GAWTC 6 cut(s) 571, 625, 703, 1266, 1335, 2348
TscAI CASTG 4 cut(s) 676, 921, 1351, 1495
TseFI GTSAC 7 cut(s) 205, 241, 280, 878, 918, 1113, 1369
Tsp45I GTSAC 7 cut(s) 205, 241, 280, 878, 918, 1113, 1369
TspGWI ACGGA 2 cut(s) 1582, 2883
TspRI CASTG 4 cut(s) 676, 921, 1351, 1495
Vha464I CTTAAG 2 cut(s) 296, 2357
VpaK11BI GGWCC 6 cut(s) 458, 890, 1757, 2258, 2270, 2401
VspI ATTAAT 1 cut(s) 863
XapI RAATTY 5 cut(s) 1661, 1974, 2117, 2606, 2623
XceI RCATGY 4 cut(s) 276, 523, 2249, 2791
XmiI GTMKAC 3 cut(s) 177, 739, 2040
XmnI GAANNNNTTC 2 cut(s) 1142, 1610
XspI CTAG 6 cut(s) 732, 1326, 1365, 1598, 2145, 3015
ZrmI AGTACT 2 cut(s) 1001, 1220
Zsp2I ATGCAT 3 cut(s) 68, 2759, 2959
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.