Rw2G014850

Alcohol dehydrogenase GroES-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001379
Physical Location & Seq
Forward (+)
16963627 .. 16964737
1111 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G014850.1

Sequence Viewer

Length: 657 bp
ATGGTTGTTCATCAAAGGTTTGTGGTTCGGATACCAGAAAAGCTAGCACCGGAGCAGGCGGCTCCATTGCTGTGTGCTGGAGTTACAGCTTACAGTCCGCTGAGGCAATTCATGGGGTCTGACAGGGTTGTAAGGGGAGGCATATTGGGTTTAGGAGGAGTTGGACACTTGGGTGTCATAATTGCAAAGGCAATGGGTCACCATGTGACTGTGATAAGCTCTTCTAATAAGAAGAGGGAAGAGGCTTTGGAGATTTTAGGTGCAGATGCTTTTATAGTTGGCTCCAATGAGGCTGAGATGGAGGGAGCTGCCAGCAGCCTTGACTATATCCTCGACACTGTACCAGCTTTGCACCCTCTGCACTCTTACCTTTCACTCCTCAAGGTTGACGGTAAGCTCATAATAGTTGCTGCTGTTCCTCAACCGCTTCAGTTTGATGCAGTTGACATAATTCTAGGGAAGAGGAGCATTTCTGGGAGCTTCATCGGAAGCATGGAAGAAACGCAGGAAATACTGGAGTTCTGGGCAGACAAGGATTTGACGACTATGATAGAGATAGTAAAACTGGATTATGCTAACATGGCATTTGAGAGAATGGAAAAAAATGATGTGAGGTTTAGGTTTGTCCTGGATGTAGCCGGAAGCAACCTGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
ADH_N ADH_zinc_N

Protein Analysis

218

Amino Acids

23.72

Weight (kDa)

5.27

Isoelectric Point (pI)

36.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_zinc_N PF00107 53 - 174 2e-22 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 86 - 205 6.4e-06 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 59, 98, 425
AcuI CTGAAG 1 cut(s) 413
AdeI CACNNNGTG 1 cut(s) 205
AfaI GTAC 1 cut(s) 342
AjnI CCWGG 2 cut(s) 627, 648
AleI CACNNNNGTG 1 cut(s) 171
AluBI AGCT 7 cut(s) 43, 89, 219, 308, 347, 397, 480
AluI AGCT 7 cut(s) 43, 89, 219, 308, 347, 397, 480
ApeKI GCWGC 3 cut(s) 308, 315, 410
AsuHPI GGTGA 1 cut(s) 191
AsuNHI GCTAGC 1 cut(s) 43
BbvCI CCTCAGC 1 cut(s) 101
BbvI GCAGC 3 cut(s) 295, 327, 397
BccI CCATC 1 cut(s) 292
BciT130I CCWGG 2 cut(s) 629, 650
BciVI GTATCC 1 cut(s) 24
BfaI CTAG 2 cut(s) 44, 455
BfuI GTATCC 1 cut(s) 24
BisI GCNGC 4 cut(s) 60, 309, 316, 411
BlsI GCNGC 4 cut(s) 61, 310, 317, 412
Bme1390I CCNGG 2 cut(s) 629, 650
BmiI GGNNCC 2 cut(s) 63, 283
BmrFI CCNGG 2 cut(s) 629, 650
BmsI GCATC 2 cut(s) 256, 427
BmtI GCTAGC 1 cut(s) 47
BpmI CTGGAG 2 cut(s) 99, 536
Bpu10I CCTNAGC 1 cut(s) 101
BpuEI CTTGAG 1 cut(s) 365
BsaWI WCCGGW 1 cut(s) 49
Bse1I ACTGG 2 cut(s) 519, 570
Bse3DI GCAATG 2 cut(s) 65, 198
BseBI CCWGG 2 cut(s) 629, 650
BseGI GGATG 1 cut(s) 637
BseMI GCAATG 2 cut(s) 65, 198
BseMII CTCAG 2 cut(s) 92, 285
BseNI ACTGG 2 cut(s) 519, 570
BseRI GAGGAG 3 cut(s) 171, 368, 478
BseXI GCAGC 3 cut(s) 295, 327, 397
BsgI GTGCAG 2 cut(s) 282, 344
BsiSI CCGG 2 cut(s) 50, 639
BspACI CCGC 3 cut(s) 59, 98, 425
BspCNI CTCAG 2 cut(s) 93, 286
BspLI GGNNCC 2 cut(s) 63, 283
BspOI GCTAGC 1 cut(s) 47
BspQI GCTCTTC 1 cut(s) 226
BsrDI GCAATG 2 cut(s) 65, 198
BsrI ACTGG 2 cut(s) 519, 570
Bst2UI CCWGG 2 cut(s) 629, 650
Bst4CI ACNGT 4 cut(s) 95, 211, 340, 392
Bst6I CTCTTC 4 cut(s) 226, 227, 234, 455
BstAPI GCANNNNNTGC 1 cut(s) 358
BstC8I GCNNGC 3 cut(s) 45, 57, 313
BstDEI CTNAG 2 cut(s) 101, 294
BstEII GGTNACC 1 cut(s) 197
BstF5I GGATG 1 cut(s) 637
BstMWI GCNNNNNNNGC 2 cut(s) 358, 581
BstNI CCWGG 2 cut(s) 629, 650
BstPI GGTNACC 1 cut(s) 197
BstSCI CCNGG 2 cut(s) 627, 648
BstV1I GCAGC 3 cut(s) 295, 327, 397
BsuI GTATCC 1 cut(s) 24
BtsCI GGATG 1 cut(s) 637
BtsIMutI CAGTG 1 cut(s) 336
Cac8I GCNNGC 3 cut(s) 45, 57, 313
Csp6I GTAC 1 cut(s) 341
CviAII CATG 4 cut(s) 112, 203, 493, 580
CviQI GTAC 1 cut(s) 341
DdeI CTNAG 2 cut(s) 101, 294
DraIII CACNNNGTG 1 cut(s) 205
Eam1104I CTCTTC 4 cut(s) 226, 227, 234, 455
EarI CTCTTC 4 cut(s) 226, 227, 234, 455
Eco57I CTGAAG 1 cut(s) 413
Eco91I GGTNACC 1 cut(s) 197
EcoO65I GGTNACC 1 cut(s) 197
EcoRII CCWGG 2 cut(s) 627, 648
FaeI CATG 4 cut(s) 115, 206, 496, 583
FatI CATG 4 cut(s) 111, 202, 492, 579
Fnu4HI GCNGC 4 cut(s) 60, 309, 316, 411
FokI GGATG 1 cut(s) 644
Fsp4HI GCNGC 4 cut(s) 60, 309, 316, 411
FspBI CTAG 2 cut(s) 44, 455
GluI GCNGC 4 cut(s) 60, 309, 316, 411
GsuI CTGGAG 2 cut(s) 99, 536
HapII CCGG 2 cut(s) 50, 639
Hin1II CATG 4 cut(s) 115, 206, 496, 583
HincII GTYRAC 2 cut(s) 388, 445
HindII GTYRAC 2 cut(s) 388, 445
HpaII CCGG 2 cut(s) 50, 639
HphI GGTGA 1 cut(s) 191
Hpy166II GTNNAC 2 cut(s) 388, 445
Hpy188I TCNGA 3 cut(s) 30, 121, 488
Hpy8I GTNNAC 2 cut(s) 388, 445
HpyCH4III ACNGT 4 cut(s) 95, 211, 340, 392
HpyCH4V TGCA 5 cut(s) 185, 263, 352, 361, 440
HpyF10VI GCNNNNNNNGC 2 cut(s) 358, 581
HpyF3I CTNAG 2 cut(s) 101, 294
Hsp92II CATG 4 cut(s) 115, 206, 496, 583
LguI GCTCTTC 1 cut(s) 226
LmnI GCTCC 6 cut(s) 52, 67, 287, 305, 465, 477
Lsp1109I GCAGC 3 cut(s) 295, 327, 397
LweI GCATC 2 cut(s) 256, 427
MaeI CTAG 2 cut(s) 44, 455
MaeIII GTNAC 3 cut(s) 82, 197, 205
MboII GAAGA 5 cut(s) 213, 244, 251, 472, 509
MluCI AATT 3 cut(s) 107, 180, 450
MmeI TCCRAC 1 cut(s) 142
MslI CAYNNNNRTG 2 cut(s) 70, 171
MspA1I CMGCKG 1 cut(s) 100
MspI CCGG 2 cut(s) 50, 639
MspR9I CCNGG 2 cut(s) 629, 650
MvaI CCWGG 2 cut(s) 629, 650
MwoI GCNNNNNNNGC 2 cut(s) 358, 581
NheI GCTAGC 1 cut(s) 43
NlaIII CATG 4 cut(s) 115, 206, 496, 583
NlaIV GGNNCC 2 cut(s) 63, 283
NmuCI GTSAC 2 cut(s) 197, 205
OliI CACNNNNGTG 1 cut(s) 171
PciSI GCTCTTC 1 cut(s) 226
PfoI TCCNGGA 1 cut(s) 627
PkrI GCNGC 4 cut(s) 61, 310, 317, 412
Psp6I CCWGG 2 cut(s) 627, 648
PspEI GGTNACC 1 cut(s) 197
PspGI CCWGG 2 cut(s) 627, 648
PspN4I GGNNCC 2 cut(s) 63, 283
RsaI GTAC 1 cut(s) 342
RsaNI GTAC 1 cut(s) 341
RseI CAYNNNNRTG 2 cut(s) 70, 171
SapI GCTCTTC 1 cut(s) 226
SatI GCNGC 4 cut(s) 60, 309, 316, 411
ScrFI CCNGG 2 cut(s) 629, 650
SfaNI GCATC 2 cut(s) 256, 427
SmiMI CAYNNNNRTG 2 cut(s) 70, 171
SmlI CTYRAG 1 cut(s) 380
SmoI CTYRAG 1 cut(s) 380
Sse9I AATT 3 cut(s) 107, 180, 450
SsiI CCGC 3 cut(s) 59, 98, 425
SspMI CTAG 2 cut(s) 44, 455
StyD4I CCNGG 2 cut(s) 627, 648
TaaI ACNGT 4 cut(s) 95, 211, 340, 392
TaqI TCGA 1 cut(s) 333
TasI AATT 3 cut(s) 107, 180, 450
TauI GCSGC 1 cut(s) 62
TscAI CASTG 1 cut(s) 343
TseFI GTSAC 2 cut(s) 197, 205
TseI GCWGC 3 cut(s) 308, 315, 410
Tsp45I GTSAC 2 cut(s) 197, 205
TspDTI ATGAA 2 cut(s) 100, 472
TspRI CASTG 1 cut(s) 343
XspI CTAG 2 cut(s) 44, 455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.