Rw2G033820

WUSCHEL-related homeobox

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001847
Physical Location & Seq
Reverse (-)
55284511 .. 55286197
1687 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G033820.1

Sequence Viewer

Length: 999 bp
ATGATGATGGGGTGTAGCAACGGTGATTGTAGAGACCCCAACAGAAACAATAATAGGGATCTGCCTGAGTACTCGATCAAAGCTGCAAATCCTCAGCCTGCAGTACAGCCAGTACTATTCACTCCAAAGCTTAGCTCTACTTCCAACATGACCCAAAACCCTGTGTTACCATTCAACCACCACCATCATCGCATGGTTCCAGTAATGTCTGATGAGCAAGGCAGAAGTAGCAGTGGTTGTTTTTATAGTGATAAACAACCGGCAGCTGGAGCTCCTCGGCGAAGCTGCCGATGGAATCCAACCCCGGAGCAGATAAAGGACCTGGAAGAGCTGTATCGATCTGGGATCAAGACACCGACGGCCCAACAAATCCGACAGGTGACTGCGCGGCTTCGAAACTATGGGAGGGTTGAAGGCAAGAATGTATTCTACTGGTTTCAGAACCACCGAGCCAGGGAGAGGCAGAAGAGACGCCGTGAACTCATCTCCATGCATCAGTCTGACACTAAACAACAGCAACAAACTTTGATTAACAGCAAGATTACCAGTCTCCAGAAAAAAGAATCTGCAGGAGTTAGCTCTGTTGGTCGTCTTGAAGTTGAGCAGCAAAATTGGCCGACCCTACCAAATTTCACTGTTCTTTCCGATCAGGAATCATCTTCAGTACTGCAGGAGAGAATGATGAGCATGGTGAAAAGCAATTCCTCCAGGCTACCTATGGAACAATCCAATTCACCTCCGACCTCATCAGACACAAACGCGTCATCAGCTACATTAATTTACACAAAGCTCTTGGACTTTCATCACTATTATGATTATGACTACGGTATGGTATTGACTGCACCCAATGTTTACAAAGAAGAAGAAAAAGAAAGCAGAGAAACTCAAACTCTAGAGCTCTTTCCACTTGAGAGCGACAATCTTAAGAGTGCTAAGCATACCAAATTACCTATGAGCAGAACAAGCACTGCAGATTTCAAGGAAGACCAATATTATTAG

Protein Analysis

332

Amino Acids

38.17

Weight (kDa)

9.01

Isoelectric Point (pI)

64.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 95 - 155 2.4e-13 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 388, 761
AciI CCGC 1 cut(s) 388
AclWI GGATC 2 cut(s) 66, 353
AcoI YGGCCR 1 cut(s) 614
AcsI RAATTY 1 cut(s) 628
AcuI CTGAAG 1 cut(s) 645
AcyI GRCGYC 1 cut(s) 472
AfaI GTAC 4 cut(s) 71, 105, 114, 666
AfiI CCNNNNNNNGG 3 cut(s) 266, 454, 459
AflII CTTAAG 1 cut(s) 923
AflIII ACRYGT 1 cut(s) 759
AgsI TTSAA 4 cut(s) 175, 413, 596, 979
AjnI CCWGG 3 cut(s) 321, 452, 707
Alw21I GWGCWC 2 cut(s) 274, 900
Alw26I GTCTC 3 cut(s) 27, 463, 554
AlwI GGATC 2 cut(s) 66, 353
AoxI GGCC 2 cut(s) 360, 614
ApeKI GCWGC 4 cut(s) 83, 263, 285, 604
ApoI RAATTY 1 cut(s) 628
AseI ATTAAT 1 cut(s) 776
Asp700I GAANNNNTTC 1 cut(s) 425
AspLEI GCGC 1 cut(s) 388
AspS9I GGNCC 2 cut(s) 319, 361
AsuC2I CCSGG 1 cut(s) 305
AsuHPI GGTGA 4 cut(s) 35, 391, 703, 726
AsuII TTCGAA 1 cut(s) 394
AvaII GGWCC 1 cut(s) 319
BanII GRGCYC 2 cut(s) 274, 900
BbsI GAAGAC 1 cut(s) 990
Bbv12I GWGCWC 2 cut(s) 274, 900
BbvCI CCTCAGC 1 cut(s) 93
BbvI GCAGC 4 cut(s) 70, 272, 275, 616
BccI CCATC 2 cut(s) 192, 285
BceAI ACGGC 2 cut(s) 375, 459
BciT130I CCWGG 3 cut(s) 323, 454, 709
BcnI CCSGG 1 cut(s) 305
BcoDI GTCTC 3 cut(s) 27, 463, 554
BfaI CTAG 1 cut(s) 893
BfmI CTRYAG 4 cut(s) 99, 567, 668, 969
BfrI CTTAAG 1 cut(s) 923
BisI GCNGC 5 cut(s) 84, 264, 286, 389, 605
BlpI GCTNAGC 2 cut(s) 131, 933
BlsI GCNGC 5 cut(s) 85, 265, 287, 390, 606
BmcAI AGTACT 3 cut(s) 71, 114, 666
Bme1390I CCNGG 4 cut(s) 305, 323, 454, 709
Bme18I GGWCC 1 cut(s) 319
BmgT120I GGNCC 2 cut(s) 319, 361
BmiI GGNNCC 1 cut(s) 198
BmrFI CCNGG 4 cut(s) 305, 323, 454, 709
BmsI GCATC 1 cut(s) 502
BpiI GAAGAC 1 cut(s) 990
BpmI CTGGAG 3 cut(s) 288, 536, 691
Bpu10I CCTNAGC 1 cut(s) 93
Bpu1102I GCTNAGC 2 cut(s) 131, 933
Bpu14I TTCGAA 1 cut(s) 394
BpuEI CTTGAG 1 cut(s) 929
BpuMI CCSGG 1 cut(s) 305
Bsa29I ATCGAT 1 cut(s) 337
BsaHI GRCGYC 1 cut(s) 472
BsaI GGTCTC 1 cut(s) 27
BsaJI CCNNGG 3 cut(s) 275, 303, 453
Bsc4I CCNNNNNNNGG 3 cut(s) 266, 454, 459
Bse118I RCCGGY 1 cut(s) 259
Bse1I ACTGG 4 cut(s) 110, 200, 437, 546
BseBI CCWGG 3 cut(s) 323, 454, 709
BseCI ATCGAT 1 cut(s) 337
BseDI CCNNGG 3 cut(s) 275, 303, 453
BseLI CCNNNNNNNGG 3 cut(s) 266, 454, 459
BseMII CTCAG 2 cut(s) 57, 107
BseNI ACTGG 4 cut(s) 110, 200, 437, 546
BseRI GAGGAG 1 cut(s) 264
BseXI GCAGC 4 cut(s) 70, 272, 275, 616
BsgI GTGCAG 1 cut(s) 825
Bsh1236I CGCG 2 cut(s) 388, 761
BshFI GGCC 2 cut(s) 362, 616
BshVI ATCGAT 1 cut(s) 337
BsiHKAI GWGCWC 2 cut(s) 274, 900
BsiSI CCGG 2 cut(s) 260, 305
BslI CCNNNNNNNGG 3 cut(s) 266, 454, 459
BsmAI GTCTC 3 cut(s) 27, 463, 554
BsmBI CGTCTC 1 cut(s) 463
BsnI GGCC 2 cut(s) 362, 616
Bso31I GGTCTC 1 cut(s) 27
Bsp119I TTCGAA 1 cut(s) 394
Bsp1286I GDGCHC 2 cut(s) 274, 900
Bsp143I GATC 5 cut(s) 58, 75, 338, 345, 646
Bsp1720I GCTNAGC 2 cut(s) 131, 933
BspACI CCGC 1 cut(s) 388
BspANI GGCC 2 cut(s) 362, 616
BspCNI CTCAG 2 cut(s) 58, 106
BspDI ATCGAT 1 cut(s) 337
BspFNI CGCG 2 cut(s) 388, 761
BspLI GGNNCC 1 cut(s) 198
BspMAI CTGCAG 4 cut(s) 103, 571, 672, 973
BspPI GGATC 2 cut(s) 66, 353
BspQI GCTCTTC 1 cut(s) 321
BspT104I TTCGAA 1 cut(s) 394
BspTI CTTAAG 1 cut(s) 923
BspTNI GGTCTC 1 cut(s) 27
BsrFI RCCGGY 1 cut(s) 259
BsrI ACTGG 4 cut(s) 110, 200, 437, 546
BssAI RCCGGY 1 cut(s) 259
BssECI CCNNGG 3 cut(s) 275, 303, 453
BssMI GATC 5 cut(s) 58, 75, 338, 345, 646
BssNI GRCGYC 1 cut(s) 472
Bst2UI CCWGG 3 cut(s) 323, 454, 709
Bst4CI ACNGT 3 cut(s) 23, 637, 827
Bst6I CTCTTC 2 cut(s) 321, 461
BstACI GRCGYC 1 cut(s) 472
BstAFI CTTAAG 1 cut(s) 923
BstBI TTCGAA 1 cut(s) 394
BstC8I GCNNGC 1 cut(s) 99
BstDEI CTNAG 4 cut(s) 66, 93, 131, 933
BstFNI CGCG 2 cut(s) 388, 761
BstHHI GCGC 1 cut(s) 388
BstKTI GATC 5 cut(s) 61, 78, 341, 348, 649
BstMAI GTCTC 3 cut(s) 27, 463, 554
BstMBI GATC 5 cut(s) 58, 75, 338, 345, 646
BstMWI GCNNNNNNNGC 5 cut(s) 228, 269, 613, 767, 963
BstNI CCWGG 3 cut(s) 323, 454, 709
BstSCI CCNGG 4 cut(s) 303, 321, 452, 707
BstSFI CTRYAG 4 cut(s) 99, 567, 668, 969
BstUI CGCG 2 cut(s) 388, 761
BstV1I GCAGC 4 cut(s) 70, 272, 275, 616
BstV2I GAAGAC 1 cut(s) 990
BstX2I RGATCY 1 cut(s) 58
BstYI RGATCY 1 cut(s) 58
Bsu15I ATCGAT 1 cut(s) 337
BsuRI GGCC 2 cut(s) 362, 616
BsuTUI ATCGAT 1 cut(s) 337
BtgZI GCGATG 1 cut(s) 173
BtsI GCAGTG 2 cut(s) 238, 966
BtsIMutI CAGTG 3 cut(s) 238, 633, 966
Cac8I GCNNGC 1 cut(s) 99
CfoI GCGC 1 cut(s) 388
Cfr10I RCCGGY 1 cut(s) 259
Cfr13I GGNCC 2 cut(s) 319, 361
ClaI ATCGAT 1 cut(s) 337
CseI GACGC 2 cut(s) 480, 750
Csp6I GTAC 4 cut(s) 70, 104, 113, 665
CviAII CATG 4 cut(s) 148, 193, 490, 688
CviQI GTAC 4 cut(s) 70, 104, 113, 665
DdeI CTNAG 4 cut(s) 66, 93, 131, 933
DpnI GATC 5 cut(s) 60, 77, 340, 347, 648
DpnII GATC 5 cut(s) 58, 75, 338, 345, 646
EaeI YGGCCR 1 cut(s) 614
Eam1104I CTCTTC 2 cut(s) 321, 461
EarI CTCTTC 2 cut(s) 321, 461
Ecl136II GAGCTC 2 cut(s) 272, 898
Eco24I GRGCYC 2 cut(s) 274, 900
Eco31I GGTCTC 1 cut(s) 27
Eco47I GGWCC 1 cut(s) 319
Eco53kI GAGCTC 2 cut(s) 272, 898
Eco57I CTGAAG 1 cut(s) 645
EcoICRI GAGCTC 2 cut(s) 272, 898
EcoO109I RGGNCCY 1 cut(s) 319
EcoRII CCWGG 3 cut(s) 321, 452, 707
EcoT22I ATGCAT 1 cut(s) 495
EcoT38I GRGCYC 2 cut(s) 274, 900
Esp3I CGTCTC 1 cut(s) 463
FaeI CATG 4 cut(s) 151, 196, 493, 691
FatI CATG 4 cut(s) 147, 192, 489, 687
Fnu4HI GCNGC 5 cut(s) 84, 264, 286, 389, 605
FriOI GRGCYC 2 cut(s) 274, 900
Fsp4HI GCNGC 5 cut(s) 84, 264, 286, 389, 605
FspBI CTAG 1 cut(s) 893
GlaI GCGC 1 cut(s) 387
GluI GCNGC 5 cut(s) 84, 264, 286, 389, 605
GsuI CTGGAG 3 cut(s) 288, 536, 691
HaeIII GGCC 2 cut(s) 362, 616
HapII CCGG 2 cut(s) 260, 305
HgaI GACGC 2 cut(s) 480, 750
HhaI GCGC 1 cut(s) 388
Hin1I GRCGYC 1 cut(s) 472
Hin1II CATG 4 cut(s) 151, 196, 493, 691
Hin6I GCGC 1 cut(s) 386
HinP1I GCGC 1 cut(s) 386
HindIII AAGCTT 1 cut(s) 128
HinfI GANTC 3 cut(s) 295, 563, 653
HpaII CCGG 2 cut(s) 260, 305
HphI GGTGA 4 cut(s) 35, 391, 703, 726
Hpy166II GTNNAC 2 cut(s) 479, 853
Hpy188I TCNGA 7 cut(s) 211, 374, 441, 502, 646, 741, 751
Hpy188III TCNNGA 5 cut(s) 349, 553, 593, 650, 893
Hpy8I GTNNAC 2 cut(s) 479, 853
Hpy99I CGWCG 1 cut(s) 361
HpyAV CCTTC 1 cut(s) 407
HpyCH4III ACNGT 3 cut(s) 23, 637, 827
HpyCH4V TGCA 7 cut(s) 86, 101, 493, 569, 670, 842, 971
HpyF10VI GCNNNNNNNGC 5 cut(s) 228, 269, 613, 767, 963
HpyF3I CTNAG 4 cut(s) 66, 93, 131, 933
Hsp92I GRCGYC 1 cut(s) 472
Hsp92II CATG 4 cut(s) 151, 196, 493, 691
HspAI GCGC 1 cut(s) 386
Kzo9I GATC 5 cut(s) 58, 75, 338, 345, 646
LguI GCTCTTC 1 cut(s) 321
LmnI GCTCC 3 cut(s) 269, 277, 307
Lsp1109I GCAGC 4 cut(s) 70, 272, 275, 616
LweI GCATC 1 cut(s) 502
MaeI CTAG 1 cut(s) 893
MaeIII GTNAC 2 cut(s) 165, 379
MalI GATC 5 cut(s) 60, 77, 340, 347, 648
MboI GATC 5 cut(s) 58, 75, 338, 345, 646
MboII GAAGA 6 cut(s) 338, 478, 651, 872, 875, 995
MflI RGATCY 1 cut(s) 58
MhlI GDGCHC 2 cut(s) 274, 900
MluCI AATT 6 cut(s) 610, 628, 700, 730, 777, 944
MluI ACGCGT 1 cut(s) 759
MmeI TCCRAC 4 cut(s) 168, 323, 397, 764
MnlI CCTC 7 cut(s) 102, 285, 399, 453, 715, 747, 754
Mph1103I ATGCAT 1 cut(s) 495
MroXI GAANNNNTTC 1 cut(s) 425
MseI TTAA 3 cut(s) 531, 776, 924
MslI CAYNNNNRTG 2 cut(s) 488, 810
MspA1I CMGCKG 1 cut(s) 266
MspCI CTTAAG 1 cut(s) 923
MspI CCGG 2 cut(s) 260, 305
MspR9I CCNGG 4 cut(s) 305, 323, 454, 709
MvaI CCWGG 3 cut(s) 323, 454, 709
MvnI CGCG 2 cut(s) 388, 761
MwoI GCNNNNNNNGC 5 cut(s) 228, 269, 613, 767, 963
NciI CCSGG 1 cut(s) 305
NdeII GATC 5 cut(s) 58, 75, 338, 345, 646
NlaIII CATG 4 cut(s) 151, 196, 493, 691
NlaIV GGNNCC 1 cut(s) 198
NmeAIII GCCGAG 1 cut(s) 256
NmuCI GTSAC 1 cut(s) 379
NsiI ATGCAT 1 cut(s) 495
NspV TTCGAA 1 cut(s) 394
PciSI GCTCTTC 1 cut(s) 321
PdmI GAANNNNTTC 1 cut(s) 425
PfeI GAWTC 3 cut(s) 295, 563, 653
PkrI GCNGC 5 cut(s) 85, 265, 287, 390, 606
PpuMI RGGWCCY 1 cut(s) 319
PshBI ATTAAT 1 cut(s) 776
Psp124BI GAGCTC 2 cut(s) 274, 900
Psp5II RGGWCCY 1 cut(s) 319
Psp6I CCWGG 3 cut(s) 321, 452, 707
PspGI CCWGG 3 cut(s) 321, 452, 707
PspN4I GGNNCC 1 cut(s) 198
PspPI GGNCC 2 cut(s) 319, 361
PspPPI RGGWCCY 1 cut(s) 319
PstI CTGCAG 4 cut(s) 103, 571, 672, 973
PsuI RGATCY 1 cut(s) 58
PvuII CAGCTG 1 cut(s) 266
RsaI GTAC 4 cut(s) 71, 105, 114, 666
RsaNI GTAC 4 cut(s) 70, 104, 113, 665
RseI CAYNNNNRTG 2 cut(s) 488, 810
SacI GAGCTC 2 cut(s) 274, 900
SapI GCTCTTC 1 cut(s) 321
SaqAI TTAA 3 cut(s) 531, 776, 924
SatI GCNGC 5 cut(s) 84, 264, 286, 389, 605
Sau3AI GATC 5 cut(s) 58, 75, 338, 345, 646
Sau96I GGNCC 2 cut(s) 319, 361
ScaI AGTACT 3 cut(s) 71, 114, 666
ScrFI CCNGG 4 cut(s) 305, 323, 454, 709
SduI GDGCHC 2 cut(s) 274, 900
SfaNI GCATC 1 cut(s) 502
SfcI CTRYAG 4 cut(s) 99, 567, 668, 969
SfuI TTCGAA 1 cut(s) 394
SinI GGWCC 1 cut(s) 319
SmiMI CAYNNNNRTG 2 cut(s) 488, 810
SmlI CTYRAG 2 cut(s) 908, 923
SmoI CTYRAG 2 cut(s) 908, 923
Sse9I AATT 6 cut(s) 610, 628, 700, 730, 777, 944
SsiI CCGC 1 cut(s) 388
SspI AATATT 1 cut(s) 992
SspMI CTAG 1 cut(s) 893
SstI GAGCTC 2 cut(s) 274, 900
StyD4I CCNGG 4 cut(s) 303, 321, 452, 707
TaaI ACNGT 3 cut(s) 23, 637, 827
TaqI TCGA 3 cut(s) 74, 337, 394
TasI AATT 6 cut(s) 610, 628, 700, 730, 777, 944
TatI WGTACW 4 cut(s) 69, 103, 112, 664
TauI GCSGC 1 cut(s) 391
TfiI GAWTC 3 cut(s) 295, 563, 653
Tru1I TTAA 3 cut(s) 531, 776, 924
Tru9I TTAA 3 cut(s) 531, 776, 924
TscAI CASTG 3 cut(s) 238, 640, 973
TseFI GTSAC 1 cut(s) 379
TseI GCWGC 4 cut(s) 83, 263, 285, 604
Tsp45I GTSAC 1 cut(s) 379
TspDTI ATGAA 1 cut(s) 791
TspRI CASTG 3 cut(s) 238, 640, 973
Vha464I CTTAAG 1 cut(s) 923
VpaK11BI GGWCC 1 cut(s) 319
VspI ATTAAT 1 cut(s) 776
XapI RAATTY 1 cut(s) 628
XbaI TCTAGA 1 cut(s) 892
XcmI CCANNNNNNNNNTGG 1 cut(s) 715
XmnI GAANNNNTTC 1 cut(s) 425
XspI CTAG 1 cut(s) 893
ZrmI AGTACT 3 cut(s) 71, 114, 666
Zsp2I ATGCAT 1 cut(s) 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.