AT1G07400

heat- shock

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0000537
Physical Location & Seq
Forward (+)
2274926 .. 2275783
858 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G07400.1

Sequence Viewer

Length: 474 bp
ATGTCGCTTATTCCAAGCTTCTTCGGCAACAACAGGCGAAGCAACAGCATCTTCGACCCGTTCTCACTCGACGTTTGGGATCCATTCAAGGAACTGCAATTCCCGTCGTCGCTCTCCGGAGAAACATCGGCGATAACGAACGCACGTGTGGACTGGAAAGAGACGGCGGAGGCGCACGTGTTCAAGGCCGATCTGCCGGGGATGAAGAAAGAGGAGGTTAAAGTGGAGATAGAGGACGACAGTGTGCTCAAGATCAGTGGAGAGAGACACGTGGAGAAAGAGGAGAAGCAGGACACGTGGCATCGTGTGGAGAGGTCGAGCGGGCAATTCTCGAGGAAGTTTAAGCTGCCGGAGAATGTGAAGATGGATCAAGTCAAGGCTTCCATGGAGAACGGAGTTCTGACTGTGACGGTGCCTAAAGTGGAAGAGGCTAAGAAGAAGGCTCAAGTTAAGTCTATTGATATCTCTGGCTAA

Protein Analysis

157

Amino Acids

17.83

Weight (kDa)

5.99

Isoelectric Point (pI)

43.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 51 - 156 8.2e-32 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000537)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07400 AT2G29500
fragaria_vesca FvH4_3g35270 FvH4_3g37330 FvH4_3g37360 FvH4_3g38620 FvH4_7g23650
malus_domestica MD07G1210800.v1.1 MD11G1087100.v1.1 MD11G1088300.v1.1 MD11G1089300.v1.1
prunus_persica Prupe.2G243400_v2.0.a1 Prupe.6G065900_v2.0.a1 Prupe.6G066100_v2.0.a1 Prupe.6G066200_v2.0.a1 Prupe.6G066300_v2.0.a1 Prupe.6G066400_v2.0.a1 Prupe.6G066500_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0368921 RchiOBHm_Chr1g0369001 RchiOBHm_Chr4g0444561 RchiOBHm_Chr5g0063321 RchiOBHm_Chr5g0063331 RchiOBHm_Chr5g0063361 RchiOBHm_Chr5g0067111 RchiOBHm_Chr5g0067191
rosa_laevigata RLG00000005821 RLG00000027165 RLG00000027176 RLG00000035622 RLG00000035623 RLG00000035626 RLG00000035628 RLG00000035914
rosa_multiflora Rmu_co8027810.1_g000001 Rmu_sc0000510.1_g000026 Rmu_sc0003839.1_g000004 Rmu_sc0004250.1_g000031 Rmu_sc0004250.1_g000043 Rmu_sc0005045.1_g000005 Rmu_sc0006795.1_g000002 Rmu_sc0008775.1_g000001 Rmu_sc0008775.1_g000012 Rmu_sc0012648.1_g000004
rosa_roxburghii Rroxscaffold_1G00013950 Rroxscaffold_1G00017380 Rroxscaffold_4G00288090 Rroxscaffold_4G00288200 Rroxscaffold_5G00385020
rosa_rugosa Rorug01G0345900 Rorug01G0346900 Rorug04G0353900 Rorug05G0356000 Rorug05G0356800 Rorug05G0381900 Rorug05G0382600
rosa_samantha Rh1AG353800 Rh1BG317300 Rh1CG331800 Rh1DG347900 Rh1DG348200 Rh4BG425600 Rh4CG440300 Rh4DG421200 Rh5AG415700 Rh5AG415800 Rh5AG416000 Rh5AG441900 Rh5BG430600 Rh5BG430700 Rh5BG431100 Rh5CG454100 Rh5CG454200 Rh5CG454400 Rh5DG444400 Rh5DG444500 Rh5DG444700
rosa_wichuraiana Rw1G031150 Rw1G031260 Rw4G035560 Rw5G039060 Rw5G039080 Rw5G041290 Rw5G041330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 412
AccBSI CCGCTC 1 cut(s) 321
AccIII TCCGGA 1 cut(s) 116
AciI CCGC 2 cut(s) 167, 321
AclWI GGATC 3 cut(s) 74, 87, 375
AcvI CACGTG 4 cut(s) 146, 178, 271, 297
AflIII ACRYGT 4 cut(s) 145, 177, 268, 294
AgsI TTSAA 2 cut(s) 88, 184
AloI GAACNNNNNNTCC 2 cut(s) 84, 116
AluBI AGCT 2 cut(s) 18, 346
AluI AGCT 2 cut(s) 18, 346
Alw21I GWGCWC 1 cut(s) 249
Alw26I GTCTC 2 cut(s) 155, 259
AlwI GGATC 3 cut(s) 74, 87, 375
Ama87I CYCGRG 1 cut(s) 331
Aor13HI TCCGGA 1 cut(s) 116
AoxI GGCC 1 cut(s) 186
ApeKI GCWGC 1 cut(s) 346
AspLEI GCGC 1 cut(s) 175
AsuC2I CCSGG 1 cut(s) 198
AvaI CYCGRG 1 cut(s) 331
BamHI GGATCC 1 cut(s) 79
BanI GGYRCC 1 cut(s) 412
BbrPI CACGTG 4 cut(s) 146, 178, 271, 297
Bbv12I GWGCWC 1 cut(s) 249
BbvI GCAGC 1 cut(s) 333
BccI CCATC 1 cut(s) 358
BceAI ACGGC 1 cut(s) 180
BcnI CCSGG 1 cut(s) 198
BcoDI GTCTC 2 cut(s) 155, 259
BisI GCNGC 1 cut(s) 347
BlsI GCNGC 1 cut(s) 348
Bme1390I CCNGG 1 cut(s) 198
BmeT110I CYCGRG 1 cut(s) 331
BmiI GGNNCC 2 cut(s) 81, 414
BmrFI CCNGG 1 cut(s) 198
BmsI GCATC 2 cut(s) 57, 310
BpuEI CTTGAG 2 cut(s) 233, 429
BpuMI CCSGG 1 cut(s) 198
BsaAI YACGTR 4 cut(s) 146, 178, 271, 297
BsaJI CCNNGG 2 cut(s) 197, 384
BsaWI WCCGGW 1 cut(s) 116
Bse1I ACTGG 1 cut(s) 158
BseAI TCCGGA 1 cut(s) 116
BseDI CCNNGG 2 cut(s) 197, 384
BseGI GGATG 1 cut(s) 207
BseNI ACTGG 1 cut(s) 158
BseRI GAGGAG 2 cut(s) 227, 296
BseXI GCAGC 1 cut(s) 333
BshFI GGCC 1 cut(s) 188
BshNI GGYRCC 1 cut(s) 412
BsiHKAI GWGCWC 1 cut(s) 249
BsiHKCI CYCGRG 1 cut(s) 331
BsiSI CCGG 3 cut(s) 117, 197, 350
BsmAI GTCTC 2 cut(s) 155, 259
BsmBI CGTCTC 1 cut(s) 155
BsnI GGCC 1 cut(s) 188
BsoBI CYCGRG 1 cut(s) 331
Bsp1286I GDGCHC 1 cut(s) 249
Bsp13I TCCGGA 1 cut(s) 116
Bsp143I GATC 4 cut(s) 79, 190, 252, 367
Bsp19I CCATGG 1 cut(s) 384
BspACI CCGC 2 cut(s) 167, 321
BspANI GGCC 1 cut(s) 188
BspEI TCCGGA 1 cut(s) 116
BspLI GGNNCC 2 cut(s) 81, 414
BspPI GGATC 3 cut(s) 74, 87, 375
BspT107I GGYRCC 1 cut(s) 412
BsrBI CCGCTC 1 cut(s) 321
BsrI ACTGG 1 cut(s) 158
BssECI CCNNGG 2 cut(s) 197, 384
BssMI GATC 4 cut(s) 79, 190, 252, 367
BssT1I CCWWGG 1 cut(s) 384
Bst4CI ACNGT 3 cut(s) 242, 406, 412
Bst6I CTCTTC 1 cut(s) 420
BstBAI YACGTR 4 cut(s) 146, 178, 271, 297
BstC8I GCNNGC 1 cut(s) 323
BstDEI CTNAG 1 cut(s) 432
BstDSI CCRYGG 1 cut(s) 384
BstF5I GGATG 1 cut(s) 207
BstHHI GCGC 1 cut(s) 175
BstKTI GATC 4 cut(s) 82, 193, 255, 370
BstMAI GTCTC 2 cut(s) 155, 259
BstMBI GATC 4 cut(s) 79, 190, 252, 367
BstMWI GCNNNNNNNGC 1 cut(s) 24
BstSCI CCNGG 1 cut(s) 196
BstV1I GCAGC 1 cut(s) 333
BstX2I RGATCY 1 cut(s) 79
BstYI RGATCY 1 cut(s) 79
BsuRI GGCC 1 cut(s) 188
BtgI CCRYGG 1 cut(s) 384
BtsCI GGATG 1 cut(s) 207
BtsIMutI CAGTG 2 cut(s) 247, 262
Cac8I GCNNGC 1 cut(s) 323
CfoI GCGC 1 cut(s) 175
CspCI CAANNNNNGTGG 2 cut(s) 238, 273
CviAII CATG 1 cut(s) 385
CviJI RGCY 7 cut(s) 18, 188, 346, 380, 431, 443, 471
CviKI_1 RGCY 7 cut(s) 18, 188, 346, 380, 431, 443, 471
DdeI CTNAG 1 cut(s) 432
DpnI GATC 4 cut(s) 81, 192, 254, 369
DpnII GATC 4 cut(s) 79, 190, 252, 367
Eam1104I CTCTTC 1 cut(s) 420
EarI CTCTTC 1 cut(s) 420
EciI GGCGGA 1 cut(s) 182
Eco130I CCWWGG 1 cut(s) 384
Eco32I GATATC 1 cut(s) 463
Eco72I CACGTG 4 cut(s) 146, 178, 271, 297
Eco88I CYCGRG 1 cut(s) 331
EcoRV GATATC 1 cut(s) 463
EcoT14I CCWWGG 1 cut(s) 384
ErhI CCWWGG 1 cut(s) 384
Esp3I CGTCTC 1 cut(s) 155
FaeI CATG 1 cut(s) 388
FaiI YATR 1 cut(s) 386
FatI CATG 1 cut(s) 384
FauI CCCGC 1 cut(s) 314
Fnu4HI GCNGC 1 cut(s) 347
FokI GGATG 1 cut(s) 214
Fsp4HI GCNGC 1 cut(s) 347
GlaI GCGC 1 cut(s) 174
GluI GCNGC 1 cut(s) 347
HaeIII GGCC 1 cut(s) 188
HapII CCGG 3 cut(s) 117, 197, 350
HhaI GCGC 1 cut(s) 175
Hin1II CATG 1 cut(s) 388
Hin6I GCGC 1 cut(s) 173
HinP1I GCGC 1 cut(s) 173
HindIII AAGCTT 1 cut(s) 16
HpaII CCGG 3 cut(s) 117, 197, 350
Hpy166II GTNNAC 1 cut(s) 151
Hpy188I TCNGA 1 cut(s) 402
Hpy188III TCNNGA 3 cut(s) 117, 250, 331
Hpy8I GTNNAC 1 cut(s) 151
Hpy99I CGWCG 3 cut(s) 74, 109, 112
HpyAV CCTTC 1 cut(s) 433
HpyCH4III ACNGT 3 cut(s) 242, 406, 412
HpyCH4IV ACGT 5 cut(s) 72, 145, 177, 270, 296
HpyCH4V TGCA 1 cut(s) 97
HpyF10VI GCNNNNNNNGC 1 cut(s) 24
HpyF3I CTNAG 1 cut(s) 432
HpySE526I ACGT 5 cut(s) 72, 145, 177, 270, 296
Hsp92II CATG 1 cut(s) 388
HspAI GCGC 1 cut(s) 173
Kpn2I TCCGGA 1 cut(s) 116
Kzo9I GATC 4 cut(s) 79, 190, 252, 367
LpnPI CCDG 7 cut(s) 19, 130, 139, 210, 275, 363, 453
Lsp1109I GCAGC 1 cut(s) 333
LweI GCATC 2 cut(s) 57, 310
MaeII ACGT 5 cut(s) 72, 145, 177, 270, 296
MaeIII GTNAC 1 cut(s) 406
MalI GATC 4 cut(s) 81, 192, 254, 369
MbiI CCGCTC 1 cut(s) 321
MboI GATC 4 cut(s) 79, 190, 252, 367
MboII GAAGA 6 cut(s) 13, 43, 217, 373, 437, 448
MflI RGATCY 1 cut(s) 79
MhlI GDGCHC 1 cut(s) 249
MluCI AATT 2 cut(s) 98, 326
MnlI CCTC 8 cut(s) 163, 205, 208, 226, 274, 306, 327, 421
MroI TCCGGA 1 cut(s) 116
MseI TTAA 3 cut(s) 219, 342, 450
MspI CCGG 3 cut(s) 117, 197, 350
MspR9I CCNGG 1 cut(s) 198
MwoI GCNNNNNNNGC 1 cut(s) 24
NciI CCSGG 1 cut(s) 198
NcoI CCATGG 1 cut(s) 384
NdeII GATC 4 cut(s) 79, 190, 252, 367
NlaIII CATG 1 cut(s) 388
NlaIV GGNNCC 2 cut(s) 81, 414
NmuCI GTSAC 1 cut(s) 406
PaeR7I CTCGAG 1 cut(s) 331
PcsI WCGNNNNNNNCGW 1 cut(s) 134
PkrI GCNGC 1 cut(s) 348
PmaCI CACGTG 4 cut(s) 146, 178, 271, 297
PmlI CACGTG 4 cut(s) 146, 178, 271, 297
Ppu21I YACGTR 4 cut(s) 146, 178, 271, 297
PspCI CACGTG 4 cut(s) 146, 178, 271, 297
PspN4I GGNNCC 2 cut(s) 81, 414
PsuI RGATCY 1 cut(s) 79
SaqAI TTAA 3 cut(s) 219, 342, 450
SatI GCNGC 1 cut(s) 347
Sau3AI GATC 4 cut(s) 79, 190, 252, 367
ScrFI CCNGG 1 cut(s) 198
SduI GDGCHC 1 cut(s) 249
SetI ASST 9 cut(s) 20, 75, 148, 180, 219, 273, 299, 317, 348
SfaNI GCATC 2 cut(s) 57, 310
Sfr274I CTCGAG 1 cut(s) 331
SlaI CTCGAG 1 cut(s) 331
SmlI CTYRAG 3 cut(s) 248, 331, 444
SmoI CTYRAG 3 cut(s) 248, 331, 444
Sse9I AATT 2 cut(s) 98, 326
SsiI CCGC 2 cut(s) 167, 321
StyD4I CCNGG 1 cut(s) 196
StyI CCWWGG 1 cut(s) 384
TaaI ACNGT 3 cut(s) 242, 406, 412
TaiI ACGT 5 cut(s) 75, 148, 180, 273, 299
TaqI TCGA 4 cut(s) 54, 69, 317, 332
TasI AATT 2 cut(s) 98, 326
Tru1I TTAA 3 cut(s) 219, 342, 450
Tru9I TTAA 3 cut(s) 219, 342, 450
TscAI CASTG 2 cut(s) 247, 262
TseFI GTSAC 1 cut(s) 406
TseI GCWGC 1 cut(s) 346
Tsp45I GTSAC 1 cut(s) 406
TspDTI ATGAA 1 cut(s) 218
TspGWI ACGGA 1 cut(s) 408
TspRI CASTG 2 cut(s) 247, 262
XhoI CTCGAG 1 cut(s) 331
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.