RchiOBHm_Chr1g0369001

response to heat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000537
Physical Location & Seq
Reverse (-)
58943779 .. 58944888
1110 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ59328

Sequence Viewer

Length: 504 bp
ATGTCGCTGATTCCCAACCCATTCGGCCGCCGATCTGGCGCATTCGGCCGCCGATCTGGCGCGTTCGACCCGTTCTCTCTTGACGCGTGGGACCACCACCCACTGGACCCCTGGCCCTTCCGCTCCGTCTCGTCGCAGTCCGAAACGGCGTCGTTCGCCCACGCGCACATCGACTGGAAGGAGACGCCGACGGCGTACGTGTTCAAGGCCGACGTGCCGGGCCTGCGCAAGGACGAGGTCAAGGTGGAGGTGGAGGACGGCAAGATCCTCCAGATCAGCGGAGAGCGGAAGCGCGATGTGGAGGAGAAGACCGACACGTGGCACCGGGTGGAACGCAGCAGCGGCAGTTTCACGAGGCGGTTCCGGCTGCCGGAGAATGCGAGAGTCGAGCAGGTCCAGGCGGCGATGGAGAACGGCGTCCTCACTGTCACGGTGCCTAAGGAGAAGGCCAAGAAGGTCGATGTCAGGTGTTCATTCGCCTATAATCTTAACGAGTTCATGTAG

Protein Analysis

167

Amino Acids

19.08

Weight (kDa)

7.9

Isoelectric Point (pI)

52.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 58 - 152 8.3e-30 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000537)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07400 AT2G29500
fragaria_vesca FvH4_3g35270 FvH4_3g37330 FvH4_3g37360 FvH4_3g38620 FvH4_7g23650
malus_domestica MD07G1210800.v1.1 MD11G1087100.v1.1 MD11G1088300.v1.1 MD11G1089300.v1.1
prunus_persica Prupe.2G243400_v2.0.a1 Prupe.6G065900_v2.0.a1 Prupe.6G066100_v2.0.a1 Prupe.6G066200_v2.0.a1 Prupe.6G066300_v2.0.a1 Prupe.6G066400_v2.0.a1 Prupe.6G066500_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0368921 RchiOBHm_Chr1g0369001 RchiOBHm_Chr4g0444561 RchiOBHm_Chr5g0063321 RchiOBHm_Chr5g0063331 RchiOBHm_Chr5g0063361 RchiOBHm_Chr5g0067111 RchiOBHm_Chr5g0067191
rosa_laevigata RLG00000005821 RLG00000027165 RLG00000027176 RLG00000035622 RLG00000035623 RLG00000035626 RLG00000035628 RLG00000035914
rosa_multiflora Rmu_co8027810.1_g000001 Rmu_sc0000510.1_g000026 Rmu_sc0003839.1_g000004 Rmu_sc0004250.1_g000031 Rmu_sc0004250.1_g000043 Rmu_sc0005045.1_g000005 Rmu_sc0006795.1_g000002 Rmu_sc0008775.1_g000001 Rmu_sc0008775.1_g000012 Rmu_sc0012648.1_g000004
rosa_roxburghii Rroxscaffold_1G00013950 Rroxscaffold_1G00017380 Rroxscaffold_4G00288090 Rroxscaffold_4G00288200 Rroxscaffold_5G00385020
rosa_rugosa Rorug01G0345900 Rorug01G0346900 Rorug04G0353900 Rorug05G0356000 Rorug05G0356800 Rorug05G0381900 Rorug05G0382600
rosa_samantha Rh1AG353800 Rh1BG317300 Rh1CG331800 Rh1DG347900 Rh1DG348200 Rh4BG425600 Rh4CG440300 Rh4DG421200 Rh5AG415700 Rh5AG415800 Rh5AG416000 Rh5AG441900 Rh5BG430600 Rh5BG430700 Rh5BG431100 Rh5CG454100 Rh5CG454200 Rh5CG454400 Rh5DG444400 Rh5DG444500 Rh5DG444700
rosa_wichuraiana Rw1G031150 Rw1G031260 Rw4G035560 Rw5G039060 Rw5G039080 Rw5G041290 Rw5G041330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 227
Acc36I ACCTGC 1 cut(s) 382
AccB1I GGYRCC 2 cut(s) 321, 433
AccB7I CCANNNNNTGG 1 cut(s) 103
AccBSI CCGCTC 2 cut(s) 123, 286
AccII CGCG 4 cut(s) 62, 86, 164, 294
AciI CCGC 8 cut(s) 28, 49, 121, 279, 286, 342, 358, 401
AclWI GGATC 1 cut(s) 259
AcoI YGGCCR 2 cut(s) 25, 46
AcvI CACGTG 1 cut(s) 318
AcyI GRCGYC 3 cut(s) 149, 185, 417
AdeI CACNNNGTG 1 cut(s) 328
AfaI GTAC 1 cut(s) 197
AfiI CCNNNNNNNGG 4 cut(s) 103, 229, 318, 370
AflIII ACRYGT 3 cut(s) 84, 198, 315
AgsI TTSAA 1 cut(s) 205
AjiI CACGTC 1 cut(s) 214
AjnI CCWGG 2 cut(s) 110, 396
Alw26I GTCTC 2 cut(s) 133, 176
AlwI GGATC 1 cut(s) 259
AoxI GGCC 6 cut(s) 25, 46, 113, 207, 220, 447
ApeKI GCWGC 3 cut(s) 336, 339, 367
AspLEI GCGC 5 cut(s) 41, 62, 166, 228, 294
AspS9I GGNCC 5 cut(s) 91, 106, 114, 220, 394
AsuC2I CCSGG 2 cut(s) 219, 326
AvaII GGWCC 3 cut(s) 91, 106, 394
AxyI CCTNAGG 1 cut(s) 438
BanI GGYRCC 2 cut(s) 321, 433
BauI CACGAG 1 cut(s) 352
BbrPI CACGTG 1 cut(s) 318
BbsI GAAGAC 1 cut(s) 314
BbvI GCAGC 3 cut(s) 348, 351, 354
BccI CCATC 1 cut(s) 400
BceAI ACGGC 4 cut(s) 162, 207, 274, 430
BciT130I CCWGG 2 cut(s) 112, 398
BcnI CCSGG 2 cut(s) 219, 326
BcoDI GTCTC 2 cut(s) 133, 176
BfuAI ACCTGC 1 cut(s) 382
BglI GCCNNNNNGGC 2 cut(s) 36, 57
BisI GCNGC 7 cut(s) 28, 49, 337, 340, 343, 368, 402
BlsI GCNGC 7 cut(s) 29, 50, 338, 341, 344, 369, 403
Bme1390I CCNGG 4 cut(s) 112, 219, 326, 398
Bme18I GGWCC 3 cut(s) 91, 106, 394
BmgBI CACGTC 1 cut(s) 214
BmgT120I GGNCC 5 cut(s) 91, 106, 114, 220, 394
BmiI GGNNCC 5 cut(s) 92, 108, 323, 362, 435
BmrFI CCNGG 4 cut(s) 112, 219, 326, 398
BpiI GAAGAC 1 cut(s) 314
BpmI CTGGAG 1 cut(s) 254
BpuMI CCSGG 2 cut(s) 219, 326
BsaAI YACGTR 2 cut(s) 199, 318
BsaHI GRCGYC 3 cut(s) 149, 185, 417
BsaJI CCNNGG 1 cut(s) 110
Bsc4I CCNNNNNNNGG 4 cut(s) 103, 229, 318, 370
Bse1I ACTGG 2 cut(s) 108, 179
Bse21I CCTNAGG 1 cut(s) 438
BseBI CCWGG 2 cut(s) 112, 398
BseDI CCNNGG 1 cut(s) 110
BseLI CCNNNNNNNGG 4 cut(s) 103, 229, 318, 370
BseNI ACTGG 2 cut(s) 108, 179
BseRI GAGGAG 1 cut(s) 317
BseX3I CGGCCG 2 cut(s) 25, 46
BseXI GCAGC 3 cut(s) 348, 351, 354
Bsh1236I CGCG 4 cut(s) 62, 86, 164, 294
Bsh1285I CGRYCG 2 cut(s) 28, 49
BshFI GGCC 6 cut(s) 27, 48, 115, 209, 222, 449
BshNI GGYRCC 2 cut(s) 321, 433
BsiEI CGRYCG 2 cut(s) 28, 49
BsiSI CCGG 4 cut(s) 218, 325, 364, 371
BsiWI CGTACG 1 cut(s) 195
BslFI GGGAC 1 cut(s) 104
BslI CCNNNNNNNGG 4 cut(s) 103, 229, 318, 370
BsmAI GTCTC 2 cut(s) 133, 176
BsmBI CGTCTC 2 cut(s) 133, 176
BsmFI GGGAC 1 cut(s) 104
BsmI GAATGC 2 cut(s) 41, 382
BsnI GGCC 6 cut(s) 27, 48, 115, 209, 222, 449
Bsp143I GATC 4 cut(s) 32, 53, 264, 273
BspACI CCGC 8 cut(s) 28, 49, 121, 279, 286, 342, 358, 401
BspANI GGCC 6 cut(s) 27, 48, 115, 209, 222, 449
BspFNI CGCG 4 cut(s) 62, 86, 164, 294
BspLI GGNNCC 5 cut(s) 92, 108, 323, 362, 435
BspMI ACCTGC 1 cut(s) 382
BspPI GGATC 1 cut(s) 259
BspT107I GGYRCC 2 cut(s) 321, 433
BsrBI CCGCTC 2 cut(s) 123, 286
BsrI ACTGG 2 cut(s) 108, 179
BssECI CCNNGG 1 cut(s) 110
BssMI GATC 4 cut(s) 32, 53, 264, 273
BssNI GRCGYC 3 cut(s) 149, 185, 417
BssSI CACGAG 1 cut(s) 352
Bst2BI CACGAG 1 cut(s) 352
Bst2UI CCWGG 2 cut(s) 112, 398
Bst4CI ACNGT 2 cut(s) 427, 433
BstACI GRCGYC 3 cut(s) 149, 185, 417
BstBAI YACGTR 2 cut(s) 199, 318
BstC8I GCNNGC 1 cut(s) 224
BstDEI CTNAG 1 cut(s) 438
BstENI CCTNNNNNAGG 1 cut(s) 227
BstFNI CGCG 4 cut(s) 62, 86, 164, 294
BstHHI GCGC 5 cut(s) 41, 62, 166, 228, 294
BstKTI GATC 4 cut(s) 35, 56, 267, 276
BstMAI GTCTC 2 cut(s) 133, 176
BstMBI GATC 4 cut(s) 32, 53, 264, 273
BstMCI CGRYCG 2 cut(s) 28, 49
BstMWI GCNNNNNNNGC 7 cut(s) 36, 45, 57, 155, 223, 342, 364
BstNI CCWGG 2 cut(s) 112, 398
BstSCI CCNGG 4 cut(s) 110, 217, 324, 396
BstUI CGCG 4 cut(s) 62, 86, 164, 294
BstV1I GCAGC 3 cut(s) 348, 351, 354
BstV2I GAAGAC 1 cut(s) 314
BstX2I RGATCY 1 cut(s) 264
BstYI RGATCY 1 cut(s) 264
BstZI CGGCCG 2 cut(s) 25, 46
Bsu36I CCTNAGG 1 cut(s) 438
BsuRI GGCC 6 cut(s) 27, 48, 115, 209, 222, 449
BtgZI GCGATG 2 cut(s) 309, 419
BtrI CACGTC 1 cut(s) 214
BtsIMutI CAGTG 2 cut(s) 101, 423
BveI ACCTGC 1 cut(s) 382
Cac8I GCNNGC 1 cut(s) 224
CfoI GCGC 5 cut(s) 41, 62, 166, 228, 294
Cfr13I GGNCC 5 cut(s) 91, 106, 114, 220, 394
CseI GACGC 4 cut(s) 92, 138, 193, 406
Csp6I GTAC 1 cut(s) 196
CviAII CATG 1 cut(s) 499
CviJI RGCY 7 cut(s) 27, 48, 115, 209, 222, 367, 449
CviKI_1 RGCY 7 cut(s) 27, 48, 115, 209, 222, 367, 449
CviQI GTAC 1 cut(s) 196
DdeI CTNAG 1 cut(s) 438
DpnI GATC 4 cut(s) 34, 55, 266, 275
DpnII GATC 4 cut(s) 32, 53, 264, 273
DraIII CACNNNGTG 1 cut(s) 328
EaeI YGGCCR 2 cut(s) 25, 46
EagI CGGCCG 2 cut(s) 25, 46
EclXI CGGCCG 2 cut(s) 25, 46
Eco47I GGWCC 3 cut(s) 91, 106, 394
Eco52I CGGCCG 2 cut(s) 25, 46
Eco72I CACGTG 1 cut(s) 318
Eco81I CCTNAGG 1 cut(s) 438
EcoNI CCTNNNNNAGG 1 cut(s) 227
EcoRII CCWGG 2 cut(s) 110, 396
Esp3I CGTCTC 2 cut(s) 133, 176
FaeI CATG 1 cut(s) 502
FaiI YATR 2 cut(s) 483, 500
FaqI GGGAC 1 cut(s) 104
FatI CATG 1 cut(s) 498
Fnu4HI GCNGC 7 cut(s) 28, 49, 337, 340, 343, 368, 402
Fsp4HI GCNGC 7 cut(s) 28, 49, 337, 340, 343, 368, 402
FspI TGCGCA 1 cut(s) 227
GlaI GCGC 5 cut(s) 40, 61, 165, 227, 293
GluI GCNGC 7 cut(s) 28, 49, 337, 340, 343, 368, 402
GsuI CTGGAG 1 cut(s) 254
HaeIII GGCC 6 cut(s) 27, 48, 115, 209, 222, 449
HapII CCGG 4 cut(s) 218, 325, 364, 371
HgaI GACGC 4 cut(s) 92, 138, 193, 406
HhaI GCGC 5 cut(s) 41, 62, 166, 228, 294
Hin1I GRCGYC 3 cut(s) 149, 185, 417
Hin1II CATG 1 cut(s) 502
Hin6I GCGC 5 cut(s) 39, 60, 164, 226, 292
HinP1I GCGC 5 cut(s) 39, 60, 164, 226, 292
HinfI GANTC 2 cut(s) 10, 384
HpaII CCGG 4 cut(s) 218, 325, 364, 371
Hpy188I TCNGA 1 cut(s) 142
Hpy188III TCNNGA 3 cut(s) 80, 271, 352
Hpy99I CGWCG 4 cut(s) 136, 154, 193, 215
HpyAV CCTTC 4 cut(s) 127, 172, 439, 448
HpyCH4III ACNGT 2 cut(s) 427, 433
HpyCH4IV ACGT 3 cut(s) 198, 213, 317
HpyF10VI GCNNNNNNNGC 7 cut(s) 36, 45, 57, 155, 223, 342, 364
HpyF3I CTNAG 1 cut(s) 438
HpySE526I ACGT 3 cut(s) 198, 213, 317
Hsp92I GRCGYC 3 cut(s) 149, 185, 417
Hsp92II CATG 1 cut(s) 502
HspAI GCGC 5 cut(s) 39, 60, 164, 226, 292
Kzo9I GATC 4 cut(s) 32, 53, 264, 273
LmnI GCTCC 1 cut(s) 128
Lsp1109I GCAGC 3 cut(s) 348, 351, 354
MaeII ACGT 3 cut(s) 198, 213, 317
MaeIII GTNAC 1 cut(s) 427
MalI GATC 4 cut(s) 34, 55, 266, 275
MbiI CCGCTC 2 cut(s) 123, 286
MboI GATC 4 cut(s) 32, 53, 264, 273
MboII GAAGA 1 cut(s) 319
MflI RGATCY 1 cut(s) 264
MluI ACGCGT 1 cut(s) 84
MlyI GAGTC 1 cut(s) 393
MnlI CCTC 7 cut(s) 229, 241, 247, 278, 295, 348, 431
MseI TTAA 1 cut(s) 489
MspA1I CMGCKG 2 cut(s) 279, 342
MspI CCGG 4 cut(s) 218, 325, 364, 371
MspR9I CCNGG 4 cut(s) 112, 219, 326, 398
Mva1269I GAATGC 2 cut(s) 41, 382
MvaI CCWGG 2 cut(s) 112, 398
MvnI CGCG 4 cut(s) 62, 86, 164, 294
MwoI GCNNNNNNNGC 7 cut(s) 36, 45, 57, 155, 223, 342, 364
NciI CCSGG 2 cut(s) 219, 326
NdeII GATC 4 cut(s) 32, 53, 264, 273
NlaIII CATG 1 cut(s) 502
NlaIV GGNNCC 5 cut(s) 92, 108, 323, 362, 435
NmuCI GTSAC 1 cut(s) 427
NsbI TGCGCA 1 cut(s) 227
PcsI WCGNNNNNNNCGW 2 cut(s) 168, 191
PctI GAATGC 2 cut(s) 41, 382
PfeI GAWTC 1 cut(s) 10
Pfl23II CGTACG 1 cut(s) 195
PflFI GACNNNGTC 1 cut(s) 236
PflMI CCANNNNNTGG 1 cut(s) 103
PkrI GCNGC 7 cut(s) 29, 50, 338, 341, 344, 369, 403
PleI GAGTC 1 cut(s) 392
PmaCI CACGTG 1 cut(s) 318
PmlI CACGTG 1 cut(s) 318
PpsI GAGTC 1 cut(s) 392
Ppu21I YACGTR 2 cut(s) 199, 318
Psp6I CCWGG 2 cut(s) 110, 396
PspCI CACGTG 1 cut(s) 318
PspGI CCWGG 2 cut(s) 110, 396
PspLI CGTACG 1 cut(s) 195
PspN4I GGNNCC 5 cut(s) 92, 108, 323, 362, 435
PspPI GGNCC 5 cut(s) 91, 106, 114, 220, 394
PsuI RGATCY 1 cut(s) 264
PsyI GACNNNGTC 1 cut(s) 236
RsaI GTAC 1 cut(s) 197
RsaNI GTAC 1 cut(s) 196
SaqAI TTAA 1 cut(s) 489
SatI GCNGC 7 cut(s) 28, 49, 337, 340, 343, 368, 402
Sau3AI GATC 4 cut(s) 32, 53, 264, 273
Sau96I GGNCC 5 cut(s) 91, 106, 114, 220, 394
SchI GAGTC 1 cut(s) 393
ScrFI CCNGG 4 cut(s) 112, 219, 326, 398
SetI ASST 9 cut(s) 201, 216, 240, 246, 252, 320, 396, 459, 470
SinI GGWCC 3 cut(s) 91, 106, 394
SsiI CCGC 8 cut(s) 28, 49, 121, 279, 286, 342, 358, 401
StyD4I CCNGG 4 cut(s) 110, 217, 324, 396
TaaI ACNGT 2 cut(s) 427, 433
TaiI ACGT 3 cut(s) 201, 216, 320
TaqI TCGA 4 cut(s) 66, 171, 387, 459
TaqII GACCGA 1 cut(s) 326
TauI GCSGC 4 cut(s) 30, 51, 345, 404
TfiI GAWTC 1 cut(s) 10
Tru1I TTAA 1 cut(s) 489
Tru9I TTAA 1 cut(s) 489
TscAI CASTG 2 cut(s) 108, 430
TseFI GTSAC 1 cut(s) 427
TseI GCWGC 3 cut(s) 336, 339, 367
Tsp45I GTSAC 1 cut(s) 427
TspDTI ATGAA 2 cut(s) 462, 487
TspGWI ACGGA 1 cut(s) 115
TspRI CASTG 2 cut(s) 108, 430
Tth111I GACNNNGTC 1 cut(s) 236
Van91I CCANNNNNTGG 1 cut(s) 103
VpaK11BI GGWCC 3 cut(s) 91, 106, 394
XagI CCTNNNNNAGG 1 cut(s) 227
XcmI CCANNNNNNNNNTGG 1 cut(s) 108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.