RchiOBHm_Chr5g0067191

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000537
Physical Location & Seq
Forward (+)
73377694 .. 73378395
702 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34292

Sequence Viewer

Length: 429 bp
ATGTCGCTCATCCCCAGCTTCCGAAGAAGCAGCGTCTTTGATCCCTTTTCCCTCAATCTCTGGGACCCTTTCAAGGACTTCCAATTCCCTGCTTCATCGCTCTCCACTTTCCCTGAATTTCCTCGCGAAAATTCGGCTTTTGTTAACACCAGGATTGACTGGAAGGAGACTCCAGAAGCCCATGTTGAGGTTGAAGATGACAGGGTGCTTCAGATTAGCGTAGAGAGGAAGATAGAGAAGGAGGACAAGAACGACACCTGGCACCGGATCGAGCGTAGCAGAGGCAAGTTCTCCAGAAGGTTCAGTCTTACTGAGAATACCAAGATAGACGAGATCAAGGCTGCTATGGAGAATGGGGTTCTCAGCGTGACAATTCCTAAGGTGGAGGTGAATAAGCCCGATGTCAAAGCTATTGAAATCTCCGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

142

Amino Acids

16.5

Weight (kDa)

5.51

Isoelectric Point (pI)

55.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 59 - 141 2.7e-19 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000537)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07400 AT2G29500
fragaria_vesca FvH4_3g35270 FvH4_3g37330 FvH4_3g37360 FvH4_3g38620 FvH4_7g23650
malus_domestica MD07G1210800.v1.1 MD11G1087100.v1.1 MD11G1088300.v1.1 MD11G1089300.v1.1
prunus_persica Prupe.2G243400_v2.0.a1 Prupe.6G065900_v2.0.a1 Prupe.6G066100_v2.0.a1 Prupe.6G066200_v2.0.a1 Prupe.6G066300_v2.0.a1 Prupe.6G066400_v2.0.a1 Prupe.6G066500_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0368921 RchiOBHm_Chr1g0369001 RchiOBHm_Chr4g0444561 RchiOBHm_Chr5g0063321 RchiOBHm_Chr5g0063331 RchiOBHm_Chr5g0063361 RchiOBHm_Chr5g0067111 RchiOBHm_Chr5g0067191
rosa_laevigata RLG00000005821 RLG00000027165 RLG00000027176 RLG00000035622 RLG00000035623 RLG00000035626 RLG00000035628 RLG00000035914
rosa_multiflora Rmu_co8027810.1_g000001 Rmu_sc0000510.1_g000026 Rmu_sc0003839.1_g000004 Rmu_sc0004250.1_g000031 Rmu_sc0004250.1_g000043 Rmu_sc0005045.1_g000005 Rmu_sc0006795.1_g000002 Rmu_sc0008775.1_g000001 Rmu_sc0008775.1_g000012 Rmu_sc0012648.1_g000004
rosa_roxburghii Rroxscaffold_1G00013950 Rroxscaffold_1G00017380 Rroxscaffold_4G00288090 Rroxscaffold_4G00288200 Rroxscaffold_5G00385020
rosa_rugosa Rorug01G0345900 Rorug01G0346900 Rorug04G0353900 Rorug05G0356000 Rorug05G0356800 Rorug05G0381900 Rorug05G0382600
rosa_samantha Rh1AG353800 Rh1BG317300 Rh1CG331800 Rh1DG347900 Rh1DG348200 Rh4BG425600 Rh4CG440300 Rh4DG421200 Rh5AG415700 Rh5AG415800 Rh5AG416000 Rh5AG441900 Rh5BG430600 Rh5BG430700 Rh5BG431100 Rh5CG454100 Rh5CG454200 Rh5CG454400 Rh5DG444400 Rh5DG444500 Rh5DG444700
rosa_wichuraiana Rw1G031150 Rw1G031260 Rw4G035560 Rw5G039060 Rw5G039080 Rw5G041290 Rw5G041330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 261
AccII CGCG 1 cut(s) 126
AclWI GGATC 2 cut(s) 35, 275
AcsI RAATTY 2 cut(s) 116, 130
AcuI CTGAAG 1 cut(s) 194
AfiI CCNNNNNNNGG 3 cut(s) 73, 187, 264
AgsI TTSAA 3 cut(s) 73, 194, 416
AjnI CCWGG 2 cut(s) 149, 257
AluBI AGCT 2 cut(s) 18, 410
AluI AGCT 2 cut(s) 18, 410
Alw26I GTCTC 1 cut(s) 161
AlwI GGATC 2 cut(s) 35, 275
ApeKI GCWGC 2 cut(s) 30, 341
ApoI RAATTY 2 cut(s) 116, 130
AspS9I GGNCC 1 cut(s) 64
AsuHPI GGTGA 1 cut(s) 400
AvaII GGWCC 1 cut(s) 64
AxyI CCTNAGG 1 cut(s) 378
BanI GGYRCC 1 cut(s) 261
BbvI GCAGC 2 cut(s) 42, 328
BciT130I CCWGG 2 cut(s) 151, 259
BcoDI GTCTC 1 cut(s) 161
BisI GCNGC 2 cut(s) 31, 342
BlsI GCNGC 2 cut(s) 32, 343
Bme1390I CCNGG 2 cut(s) 151, 259
Bme18I GGWCC 1 cut(s) 64
BmgT120I GGNCC 1 cut(s) 64
BmiI GGNNCC 3 cut(s) 65, 66, 263
BmrFI CCNGG 2 cut(s) 151, 259
BpmI CTGGAG 2 cut(s) 156, 277
BsaWI WCCGGW 2 cut(s) 264, 422
Bsc4I CCNNNNNNNGG 3 cut(s) 73, 187, 264
Bse1I ACTGG 1 cut(s) 164
Bse21I CCTNAGG 1 cut(s) 378
BseBI CCWGG 2 cut(s) 151, 259
BseGI GGATG 1 cut(s) 9
BseLI CCNNNNNNNGG 3 cut(s) 73, 187, 264
BseMII CTCAG 2 cut(s) 303, 376
BseNI ACTGG 1 cut(s) 164
BseXI GCAGC 2 cut(s) 42, 328
BseYI CCCAGC 1 cut(s) 14
Bsh1236I CGCG 1 cut(s) 126
BshNI GGYRCC 1 cut(s) 261
BsiSI CCGG 2 cut(s) 265, 423
BslFI GGGAC 1 cut(s) 77
BslI CCNNNNNNNGG 3 cut(s) 73, 187, 264
BsmAI GTCTC 1 cut(s) 161
BsmFI GGGAC 1 cut(s) 77
Bsp143I GATC 3 cut(s) 40, 267, 333
Bsp68I TCGCGA 1 cut(s) 126
BspCNI CTCAG 2 cut(s) 304, 375
BspFNI CGCG 1 cut(s) 126
BspLI GGNNCC 3 cut(s) 65, 66, 263
BspPI GGATC 2 cut(s) 35, 275
BspT107I GGYRCC 1 cut(s) 261
BsrI ACTGG 1 cut(s) 164
BssMI GATC 3 cut(s) 40, 267, 333
Bst2UI CCWGG 2 cut(s) 151, 259
BstDEI CTNAG 3 cut(s) 312, 362, 378
BstF5I GGATG 1 cut(s) 9
BstFNI CGCG 1 cut(s) 126
BstKTI GATC 3 cut(s) 43, 270, 336
BstMAI GTCTC 1 cut(s) 161
BstMBI GATC 3 cut(s) 40, 267, 333
BstNI CCWGG 2 cut(s) 151, 259
BstSCI CCNGG 2 cut(s) 149, 257
BstUI CGCG 1 cut(s) 126
BstV1I GCAGC 2 cut(s) 42, 328
Bsu36I CCTNAGG 1 cut(s) 378
BtgZI GCGATG 1 cut(s) 81
BtsCI GGATG 1 cut(s) 9
BtuMI TCGCGA 1 cut(s) 126
Cfr13I GGNCC 1 cut(s) 64
CseI GACGC 1 cut(s) 22
CviAII CATG 1 cut(s) 182
CviJI RGCY 6 cut(s) 18, 137, 179, 341, 397, 410
CviKI_1 RGCY 6 cut(s) 18, 137, 179, 341, 397, 410
DdeI CTNAG 3 cut(s) 312, 362, 378
DpnI GATC 3 cut(s) 42, 269, 335
DpnII GATC 3 cut(s) 40, 267, 333
Eco47I GGWCC 1 cut(s) 64
Eco57I CTGAAG 1 cut(s) 194
Eco81I CCTNAGG 1 cut(s) 378
EcoO109I RGGNCCY 1 cut(s) 64
EcoRII CCWGG 2 cut(s) 149, 257
FaeI CATG 1 cut(s) 185
FaiI YATR 2 cut(s) 183, 347
FaqI GGGAC 1 cut(s) 77
FatI CATG 1 cut(s) 181
Fnu4HI GCNGC 2 cut(s) 31, 342
Fsp4HI GCNGC 2 cut(s) 31, 342
GluI GCNGC 2 cut(s) 31, 342
GsaI CCCAGC 1 cut(s) 18
GsuI CTGGAG 2 cut(s) 156, 277
HapII CCGG 2 cut(s) 265, 423
HgaI GACGC 1 cut(s) 22
Hin1II CATG 1 cut(s) 185
HincII GTYRAC 1 cut(s) 145
HindII GTYRAC 1 cut(s) 145
HinfI GANTC 1 cut(s) 169
HpaI GTTAAC 1 cut(s) 145
HpaII CCGG 2 cut(s) 265, 423
HphI GGTGA 1 cut(s) 400
Hpy166II GTNNAC 1 cut(s) 145
Hpy188I TCNGA 2 cut(s) 23, 213
Hpy188III TCNNGA 3 cut(s) 125, 173, 294
Hpy8I GTNNAC 1 cut(s) 145
HpyAV CCTTC 3 cut(s) 157, 232, 291
HpyF3I CTNAG 3 cut(s) 312, 362, 378
Hsp92II CATG 1 cut(s) 185
KflI GGGWCCC 1 cut(s) 64
KspAI GTTAAC 1 cut(s) 145
Kzo9I GATC 3 cut(s) 40, 267, 333
Lsp1109I GCAGC 2 cut(s) 42, 328
MaeIII GTNAC 1 cut(s) 367
MalI GATC 3 cut(s) 42, 269, 335
MboI GATC 3 cut(s) 40, 267, 333
MboII GAAGA 3 cut(s) 36, 206, 241
MluCI AATT 4 cut(s) 83, 116, 130, 372
MlyI GAGTC 1 cut(s) 163
MnlI CCTC 7 cut(s) 62, 132, 181, 219, 235, 275, 379
MseI TTAA 1 cut(s) 144
MspI CCGG 2 cut(s) 265, 423
MspR9I CCNGG 2 cut(s) 151, 259
MvaI CCWGG 2 cut(s) 151, 259
MvnI CGCG 1 cut(s) 126
NdeII GATC 3 cut(s) 40, 267, 333
NlaIII CATG 1 cut(s) 185
NlaIV GGNNCC 3 cut(s) 65, 66, 263
NmuCI GTSAC 1 cut(s) 367
NruI TCGCGA 1 cut(s) 126
PkrI GCNGC 2 cut(s) 32, 343
PleI GAGTC 1 cut(s) 163
PpsI GAGTC 1 cut(s) 163
PpuMI RGGWCCY 1 cut(s) 64
Psp5II RGGWCCY 1 cut(s) 64
Psp6I CCWGG 2 cut(s) 149, 257
PspFI CCCAGC 1 cut(s) 14
PspGI CCWGG 2 cut(s) 149, 257
PspN4I GGNNCC 3 cut(s) 65, 66, 263
PspPI GGNCC 1 cut(s) 64
PspPPI RGGWCCY 1 cut(s) 64
RruI TCGCGA 1 cut(s) 126
SaqAI TTAA 1 cut(s) 144
SatI GCNGC 2 cut(s) 31, 342
Sau3AI GATC 3 cut(s) 40, 267, 333
Sau96I GGNCC 1 cut(s) 64
SchI GAGTC 1 cut(s) 163
ScrFI CCNGG 2 cut(s) 151, 259
SetI ASST 7 cut(s) 20, 192, 260, 302, 384, 390, 412
SinI GGWCC 1 cut(s) 64
Sse9I AATT 4 cut(s) 83, 116, 130, 372
StyD4I CCNGG 2 cut(s) 149, 257
TaqI TCGA 1 cut(s) 270
TasI AATT 4 cut(s) 83, 116, 130, 372
Tru1I TTAA 1 cut(s) 144
Tru9I TTAA 1 cut(s) 144
TseFI GTSAC 1 cut(s) 367
TseI GCWGC 2 cut(s) 30, 341
Tsp45I GTSAC 1 cut(s) 367
TspDTI ATGAA 1 cut(s) 84
VpaK11BI GGWCC 1 cut(s) 64
XapI RAATTY 2 cut(s) 116, 130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.