MD11G1089300.v1.1

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0000537
Physical Location & Seq
Reverse (-)
7421465 .. 7421947
483 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1089300.v1.1.491

Sequence Viewer

Length: 483 bp
ATGTCGCTAATTCCCAACTCCCGACGAGGAAGCAGCAGCGTCTTCGACCCATTCTCCCTCAATCTGTGGGACCCTTTCAAGGATTTCCCGTTCCCTTCCTCCTCATCACTCTCCGCATTTCCTGAATTTTCTCGGGAGAATTCGGCTTTTGTGAACACTAGGGTCGACTGGAAGGAGACCCCCGAAGCCCATGTGTTCAAGGCGGACGTTCCGGGGCTGAAAAAAGAGGAGGTGAAGGTGGAGGTGGAAGACGACAGGGTGCTTAAGATCAGCGGAGAGAGGAACGTGGAGGAGGAGGATAAAAACGACAAGTGGTACAGAGTGGAGAGGAGCAGCGGCAAGTTCTTGAGGAGGTTTCAGCTTCCGGAGAACGCAAAGGTTGATCAGATTAAGGCTGCCATGGAGAATGGAGTTCTAAGTGTCACTGTTCCGAAGGCGGAGTTGAAGAACGTTGACGTCAGAGCCATTGAAATTTCGGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

161

Amino Acids

18.22

Weight (kDa)

5.39

Isoelectric Point (pI)

47.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 56 - 159 1.4e-31 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000537)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07400 AT2G29500
fragaria_vesca FvH4_3g35270 FvH4_3g37330 FvH4_3g37360 FvH4_3g38620 FvH4_7g23650
malus_domestica MD07G1210800.v1.1 MD11G1087100.v1.1 MD11G1088300.v1.1 MD11G1089300.v1.1
prunus_persica Prupe.2G243400_v2.0.a1 Prupe.6G065900_v2.0.a1 Prupe.6G066100_v2.0.a1 Prupe.6G066200_v2.0.a1 Prupe.6G066300_v2.0.a1 Prupe.6G066400_v2.0.a1 Prupe.6G066500_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0368921 RchiOBHm_Chr1g0369001 RchiOBHm_Chr4g0444561 RchiOBHm_Chr5g0063321 RchiOBHm_Chr5g0063331 RchiOBHm_Chr5g0063361 RchiOBHm_Chr5g0067111 RchiOBHm_Chr5g0067191
rosa_laevigata RLG00000005821 RLG00000027165 RLG00000027176 RLG00000035622 RLG00000035623 RLG00000035626 RLG00000035628 RLG00000035914
rosa_multiflora Rmu_co8027810.1_g000001 Rmu_sc0000510.1_g000026 Rmu_sc0003839.1_g000004 Rmu_sc0004250.1_g000031 Rmu_sc0004250.1_g000043 Rmu_sc0005045.1_g000005 Rmu_sc0006795.1_g000002 Rmu_sc0008775.1_g000001 Rmu_sc0008775.1_g000012 Rmu_sc0012648.1_g000004
rosa_roxburghii Rroxscaffold_1G00013950 Rroxscaffold_1G00017380 Rroxscaffold_4G00288090 Rroxscaffold_4G00288200 Rroxscaffold_5G00385020
rosa_rugosa Rorug01G0345900 Rorug01G0346900 Rorug04G0353900 Rorug05G0356000 Rorug05G0356800 Rorug05G0381900 Rorug05G0382600
rosa_samantha Rh1AG353800 Rh1BG317300 Rh1CG331800 Rh1DG347900 Rh1DG348200 Rh4BG425600 Rh4CG440300 Rh4DG421200 Rh5AG415700 Rh5AG415800 Rh5AG416000 Rh5AG441900 Rh5BG430600 Rh5BG430700 Rh5BG431100 Rh5CG454100 Rh5CG454200 Rh5CG454400 Rh5DG444400 Rh5DG444500 Rh5DG444700
rosa_wichuraiana Rw1G031150 Rw1G031260 Rw4G035560 Rw5G039060 Rw5G039080 Rw5G041290 Rw5G041330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 459
AccI GTMKAC 1 cut(s) 165
AccIII TCCGGA 1 cut(s) 364
AciI CCGC 5 cut(s) 114, 203, 273, 336, 437
AclI AACGTT 1 cut(s) 450
AcsI RAATTY 3 cut(s) 125, 139, 471
AcyI GRCGYC 1 cut(s) 456
AfaI GTAC 1 cut(s) 317
AfiI CCNNNNNNNGG 1 cut(s) 79
AflII CTTAAG 1 cut(s) 263
AgsI TTSAA 4 cut(s) 79, 199, 445, 470
AloI GAACNNNNNNTCC 2 cut(s) 74, 106
AluBI AGCT 1 cut(s) 361
AluI AGCT 1 cut(s) 361
Alw26I GTCTC 1 cut(s) 170
Ama87I CYCGRG 1 cut(s) 132
Aor13HI TCCGGA 1 cut(s) 364
ApeKI GCWGC 4 cut(s) 33, 36, 333, 395
ApoI RAATTY 3 cut(s) 125, 139, 471
AspS9I GGNCC 1 cut(s) 70
AsuC2I CCSGG 1 cut(s) 213
AsuHPI GGTGA 1 cut(s) 244
AvaI CYCGRG 1 cut(s) 132
AvaII GGWCC 1 cut(s) 70
BbsI GAAGAC 2 cut(s) 34, 255
BbvI GCAGC 4 cut(s) 45, 48, 345, 382
BcgI CGANNNNNNTGC 2 cut(s) 25, 59
BclI TGATCA 1 cut(s) 382
BcnI CCSGG 1 cut(s) 213
BcoDI GTCTC 1 cut(s) 170
BfaI CTAG 1 cut(s) 159
BfrI CTTAAG 1 cut(s) 263
BisI GCNGC 5 cut(s) 34, 37, 334, 337, 396
BlsI GCNGC 5 cut(s) 35, 38, 335, 338, 397
Bme1390I CCNGG 1 cut(s) 213
Bme18I GGWCC 1 cut(s) 70
BmeT110I CYCGRG 1 cut(s) 132
BmgT120I GGNCC 1 cut(s) 70
BmiI GGNNCC 2 cut(s) 71, 72
BmrFI CCNGG 1 cut(s) 213
BpiI GAAGAC 2 cut(s) 34, 255
BpuEI CTTGAG 1 cut(s) 367
BpuMI CCSGG 1 cut(s) 213
BsaHI GRCGYC 1 cut(s) 456
BsaI GGTCTC 1 cut(s) 170
BsaJI CCNNGG 2 cut(s) 212, 399
BsaWI WCCGGW 1 cut(s) 364
BsaXI ACNNNNNCTCC 2 cut(s) 38, 68
Bsc4I CCNNNNNNNGG 1 cut(s) 79
Bse1I ACTGG 1 cut(s) 173
BseAI TCCGGA 1 cut(s) 364
BseDI CCNNGG 2 cut(s) 212, 399
BseLI CCNNNNNNNGG 1 cut(s) 79
BseNI ACTGG 1 cut(s) 173
BseRI GAGGAG 6 cut(s) 91, 242, 305, 308, 343, 364
BseXI GCAGC 4 cut(s) 45, 48, 345, 382
BsiHKCI CYCGRG 1 cut(s) 132
BsiSI CCGG 2 cut(s) 212, 365
BslFI GGGAC 1 cut(s) 83
BslI CCNNNNNNNGG 1 cut(s) 79
BsmAI GTCTC 1 cut(s) 170
BsmFI GGGAC 1 cut(s) 83
Bso31I GGTCTC 1 cut(s) 170
BsoBI CYCGRG 1 cut(s) 132
Bsp13I TCCGGA 1 cut(s) 364
Bsp143I GATC 2 cut(s) 267, 382
Bsp19I CCATGG 1 cut(s) 399
BspACI CCGC 5 cut(s) 114, 203, 273, 336, 437
BspEI TCCGGA 1 cut(s) 364
BspLI GGNNCC 2 cut(s) 71, 72
BspTI CTTAAG 1 cut(s) 263
BspTNI GGTCTC 1 cut(s) 170
BsrI ACTGG 1 cut(s) 173
BssECI CCNNGG 2 cut(s) 212, 399
BssMI GATC 2 cut(s) 267, 382
BssNI GRCGYC 1 cut(s) 456
BssT1I CCWWGG 1 cut(s) 399
Bst4CI ACNGT 1 cut(s) 427
BstACI GRCGYC 1 cut(s) 456
BstAFI CTTAAG 1 cut(s) 263
BstDEI CTNAG 1 cut(s) 416
BstDSI CCRYGG 1 cut(s) 399
BstKTI GATC 2 cut(s) 270, 385
BstMAI GTCTC 1 cut(s) 170
BstMBI GATC 2 cut(s) 267, 382
BstSCI CCNGG 1 cut(s) 211
BstV1I GCAGC 4 cut(s) 45, 48, 345, 382
BstV2I GAAGAC 2 cut(s) 34, 255
BtgI CCRYGG 1 cut(s) 399
BtsIMutI CAGTG 1 cut(s) 423
Cfr13I GGNCC 1 cut(s) 70
CseI GACGC 1 cut(s) 28
Csp6I GTAC 1 cut(s) 316
CviAII CATG 2 cut(s) 191, 400
CviJI RGCY 6 cut(s) 146, 188, 217, 361, 395, 464
CviKI_1 RGCY 6 cut(s) 146, 188, 217, 361, 395, 464
CviQI GTAC 1 cut(s) 316
DdeI CTNAG 1 cut(s) 416
DpnI GATC 2 cut(s) 269, 384
DpnII GATC 2 cut(s) 267, 382
EciI GGCGGA 2 cut(s) 218, 452
Eco130I CCWWGG 1 cut(s) 399
Eco31I GGTCTC 1 cut(s) 170
Eco47I GGWCC 1 cut(s) 70
Eco88I CYCGRG 1 cut(s) 132
EcoO109I RGGNCCY 1 cut(s) 70
EcoRI GAATTC 1 cut(s) 139
EcoT14I CCWWGG 1 cut(s) 399
ErhI CCWWGG 1 cut(s) 399
FaeI CATG 2 cut(s) 194, 403
FaiI YATR 2 cut(s) 192, 401
FaqI GGGAC 1 cut(s) 83
FatI CATG 2 cut(s) 190, 399
FbaI TGATCA 1 cut(s) 382
FblI GTMKAC 1 cut(s) 165
Fnu4HI GCNGC 5 cut(s) 34, 37, 334, 337, 396
Fsp4HI GCNGC 5 cut(s) 34, 37, 334, 337, 396
FspBI CTAG 1 cut(s) 159
GluI GCNGC 5 cut(s) 34, 37, 334, 337, 396
HapII CCGG 2 cut(s) 212, 365
HgaI GACGC 1 cut(s) 28
Hin1I GRCGYC 1 cut(s) 456
Hin1II CATG 2 cut(s) 194, 403
HincII GTYRAC 2 cut(s) 166, 454
HindII GTYRAC 2 cut(s) 166, 454
HpaII CCGG 2 cut(s) 212, 365
HphI GGTGA 1 cut(s) 244
Hpy166II GTNNAC 3 cut(s) 154, 166, 454
Hpy188I TCNGA 3 cut(s) 387, 432, 461
Hpy188III TCNNGA 5 cut(s) 21, 122, 134, 346, 365
Hpy8I GTNNAC 3 cut(s) 154, 166, 454
Hpy99I CGWCG 1 cut(s) 27
HpyAV CCTTC 4 cut(s) 105, 166, 229, 427
HpyCH4III ACNGT 1 cut(s) 427
HpyCH4IV ACGT 4 cut(s) 207, 285, 450, 456
HpyF3I CTNAG 1 cut(s) 416
HpySE526I ACGT 4 cut(s) 207, 285, 450, 456
Hsp92I GRCGYC 1 cut(s) 456
Hsp92II CATG 2 cut(s) 194, 403
KflI GGGWCCC 1 cut(s) 70
Kpn2I TCCGGA 1 cut(s) 364
Ksp22I TGATCA 1 cut(s) 382
Kzo9I GATC 2 cut(s) 267, 382
LmnI GCTCC 1 cut(s) 330
LpnPI CCDG 5 cut(s) 135, 154, 225, 241, 378
Lsp1109I GCAGC 4 cut(s) 45, 48, 345, 382
MaeI CTAG 1 cut(s) 159
MaeII ACGT 4 cut(s) 207, 285, 450, 456
MaeIII GTNAC 1 cut(s) 421
MalI GATC 2 cut(s) 269, 384
MboI GATC 2 cut(s) 267, 382
MboII GAAGA 3 cut(s) 34, 260, 457
MluCI AATT 4 cut(s) 9, 125, 139, 471
MroI TCCGGA 1 cut(s) 364
MseI TTAA 2 cut(s) 264, 390
MspA1I CMGCKG 2 cut(s) 273, 336
MspCI CTTAAG 1 cut(s) 263
MspI CCGG 2 cut(s) 212, 365
MspR9I CCNGG 1 cut(s) 213
NciI CCSGG 1 cut(s) 213
NcoI CCATGG 1 cut(s) 399
NdeII GATC 2 cut(s) 267, 382
NlaIII CATG 2 cut(s) 194, 403
NlaIV GGNNCC 2 cut(s) 71, 72
NmuCI GTSAC 1 cut(s) 421
PkrI GCNGC 5 cut(s) 35, 38, 335, 338, 397
PpuMI RGGWCCY 1 cut(s) 70
Psp1406I AACGTT 1 cut(s) 450
Psp5II RGGWCCY 1 cut(s) 70
PspN4I GGNNCC 2 cut(s) 71, 72
PspPI GGNCC 1 cut(s) 70
PspPPI RGGWCCY 1 cut(s) 70
RsaI GTAC 1 cut(s) 317
RsaNI GTAC 1 cut(s) 316
SalI GTCGAC 1 cut(s) 164
SaqAI TTAA 2 cut(s) 264, 390
SatI GCNGC 5 cut(s) 34, 37, 334, 337, 396
Sau3AI GATC 2 cut(s) 267, 382
Sau96I GGNCC 1 cut(s) 70
ScrFI CCNGG 1 cut(s) 213
SinI GGWCC 1 cut(s) 70
SmlI CTYRAG 2 cut(s) 263, 346
SmoI CTYRAG 2 cut(s) 263, 346
Sse9I AATT 4 cut(s) 9, 125, 139, 471
SsiI CCGC 5 cut(s) 114, 203, 273, 336, 437
SspMI CTAG 1 cut(s) 159
StyD4I CCNGG 1 cut(s) 211
StyI CCWWGG 1 cut(s) 399
TaaI ACNGT 1 cut(s) 427
TaiI ACGT 4 cut(s) 210, 288, 453, 459
TaqI TCGA 2 cut(s) 45, 165
TasI AATT 4 cut(s) 9, 125, 139, 471
TauI GCSGC 1 cut(s) 339
Tru1I TTAA 2 cut(s) 264, 390
Tru9I TTAA 2 cut(s) 264, 390
TscAI CASTG 1 cut(s) 430
TseFI GTSAC 1 cut(s) 421
TseI GCWGC 4 cut(s) 33, 36, 333, 395
Tsp45I GTSAC 1 cut(s) 421
TspRI CASTG 1 cut(s) 430
Vha464I CTTAAG 1 cut(s) 263
VpaK11BI GGWCC 1 cut(s) 70
XapI RAATTY 3 cut(s) 125, 139, 471
XmiI GTMKAC 1 cut(s) 165
XspI CTAG 1 cut(s) 159
ZraI GACGTC 1 cut(s) 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.