Hsp20/alpha crystallin family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 603 |
| AccI | GTMKAC | 1 cut(s) | 1076 |
| AclWI | GGATC | 1 cut(s) | 68 |
| AcoI | YGGCCR | 1 cut(s) | 3 |
| AcsI | RAATTY | 1 cut(s) | 852 |
| AcyI | GRCGYC | 1 cut(s) | 631 |
| AfaI | GTAC | 3 cut(s) | 115, 269, 749 |
| AfeI | AGCGCT | 1 cut(s) | 154 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 57, 485, 603 |
| AgsI | TTSAA | 5 cut(s) | 120, 364, 464, 766, 926 |
| AjnI | CCWGG | 2 cut(s) | 50, 602 |
| AleI | CACNNNNGTG | 1 cut(s) | 605 |
| AluBI | AGCT | 4 cut(s) | 139, 662, 912, 1019 |
| AluI | AGCT | 4 cut(s) | 139, 662, 912, 1019 |
| Alw21I | GWGCWC | 1 cut(s) | 914 |
| Alw26I | GTCTC | 3 cut(s) | 540, 830, 998 |
| AlwI | GGATC | 1 cut(s) | 68 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 603 |
| Aor51HI | AGCGCT | 1 cut(s) | 154 |
| AoxI | GGCC | 2 cut(s) | 3, 60 |
| ApeKI | GCWGC | 1 cut(s) | 1016 |
| ApoI | RAATTY | 1 cut(s) | 852 |
| AseI | ATTAAT | 1 cut(s) | 108 |
| AspLEI | GCGC | 1 cut(s) | 155 |
| AspS9I | GGNCC | 2 cut(s) | 60, 305 |
| AsuHPI | GGTGA | 1 cut(s) | 619 |
| AvaII | GGWCC | 1 cut(s) | 305 |
| BalI | TGGCCA | 1 cut(s) | 5 |
| BanII | GRGCYC | 1 cut(s) | 914 |
| BbsI | GAAGAC | 1 cut(s) | 954 |
| Bbv12I | GWGCWC | 1 cut(s) | 914 |
| BbvI | GCAGC | 1 cut(s) | 1028 |
| BccI | CCATC | 4 cut(s) | 389, 848, 959, 1091 |
| BceAI | ACGGC | 1 cut(s) | 967 |
| BciT130I | CCWGG | 2 cut(s) | 52, 604 |
| BclI | TGATCA | 3 cut(s) | 226, 246, 469 |
| BcoDI | GTCTC | 3 cut(s) | 540, 830, 998 |
| BfaI | CTAG | 4 cut(s) | 20, 588, 713, 887 |
| BfmI | CTRYAG | 1 cut(s) | 347 |
| BfoI | RGCGCY | 1 cut(s) | 156 |
| BisI | GCNGC | 1 cut(s) | 1017 |
| BlsI | GCNGC | 1 cut(s) | 1018 |
| Bme1390I | CCNGG | 2 cut(s) | 52, 604 |
| Bme18I | GGWCC | 1 cut(s) | 305 |
| BmgT120I | GGNCC | 2 cut(s) | 60, 305 |
| BmrFI | CCNGG | 2 cut(s) | 52, 604 |
| BmsI | GCATC | 5 cut(s) | 342, 530, 819, 888, 1058 |
| BpiI | GAAGAC | 1 cut(s) | 954 |
| BpmI | CTGGAG | 1 cut(s) | 1049 |
| BpuEI | CTTGAG | 1 cut(s) | 854 |
| BsaBI | GATNNNNATC | 3 cut(s) | 231, 248, 474 |
| BsaHI | GRCGYC | 1 cut(s) | 631 |
| BsaI | GGTCTC | 1 cut(s) | 830 |
| BsaJI | CCNNGG | 4 cut(s) | 238, 603, 674, 1011 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 57, 485, 603 |
| Bse1I | ACTGG | 2 cut(s) | 554, 990 |
| Bse8I | GATNNNNATC | 3 cut(s) | 231, 248, 474 |
| BseBI | CCWGG | 2 cut(s) | 52, 604 |
| BseDI | CCNNGG | 4 cut(s) | 238, 603, 674, 1011 |
| BseGI | GGATG | 7 cut(s) | 249, 333, 381, 834, 840, 882, 1049 |
| BseJI | GATNNNNATC | 3 cut(s) | 231, 248, 474 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 57, 485, 603 |
| BseMII | CTCAG | 5 cut(s) | 177, 403, 654, 927, 1011 |
| BseNI | ACTGG | 2 cut(s) | 554, 990 |
| BseRI | GAGGAG | 1 cut(s) | 377 |
| BseXI | GCAGC | 1 cut(s) | 1028 |
| BseYI | CCCAGC | 1 cut(s) | 135 |
| BshFI | GGCC | 2 cut(s) | 5, 62 |
| BsiHKAI | GWGCWC | 1 cut(s) | 914 |
| BslFI | GGGAC | 1 cut(s) | 1090 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 57, 485, 603 |
| BsmAI | GTCTC | 3 cut(s) | 540, 830, 998 |
| BsmFI | GGGAC | 1 cut(s) | 1090 |
| BsmI | GAATGC | 2 cut(s) | 459, 954 |
| BsnI | GGCC | 2 cut(s) | 5, 62 |
| Bso31I | GGTCTC | 1 cut(s) | 830 |
| Bsp1286I | GDGCHC | 1 cut(s) | 914 |
| Bsp143I | GATC | 5 cut(s) | 73, 226, 246, 430, 469 |
| BspANI | GGCC | 2 cut(s) | 5, 62 |
| BspCNI | CTCAG | 5 cut(s) | 176, 402, 655, 926, 1012 |
| BspMAI | CTGCAG | 1 cut(s) | 351 |
| BspPI | GGATC | 1 cut(s) | 68 |
| BspQI | GCTCTTC | 1 cut(s) | 80 |
| BspTNI | GGTCTC | 1 cut(s) | 830 |
| BsrI | ACTGG | 2 cut(s) | 554, 990 |
| BssECI | CCNNGG | 4 cut(s) | 238, 603, 674, 1011 |
| BssMI | GATC | 5 cut(s) | 73, 226, 246, 430, 469 |
| BssNAI | GTATAC | 1 cut(s) | 1077 |
| BssNI | GRCGYC | 1 cut(s) | 631 |
| BssT1I | CCWWGG | 3 cut(s) | 238, 674, 1011 |
| Bst1107I | GTATAC | 1 cut(s) | 1077 |
| Bst2UI | CCWGG | 2 cut(s) | 52, 604 |
| Bst4CI | ACNGT | 3 cut(s) | 319, 933, 1074 |
| Bst6I | CTCTTC | 1 cut(s) | 80 |
| BstACI | GRCGYC | 1 cut(s) | 631 |
| BstC8I | GCNNGC | 3 cut(s) | 64, 151, 693 |
| BstDEI | CTNAG | 5 cut(s) | 163, 389, 663, 913, 1020 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 55 |
| BstF5I | GGATG | 7 cut(s) | 249, 333, 381, 834, 840, 882, 1049 |
| BstH2I | RGCGCY | 1 cut(s) | 156 |
| BstHHI | GCGC | 1 cut(s) | 155 |
| BstKTI | GATC | 5 cut(s) | 76, 229, 249, 433, 472 |
| BstMAI | GTCTC | 3 cut(s) | 540, 830, 998 |
| BstMBI | GATC | 5 cut(s) | 73, 226, 246, 430, 469 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 668 |
| BstNI | CCWGG | 2 cut(s) | 52, 604 |
| BstNSI | RCATGY | 1 cut(s) | 803 |
| BstSCI | CCNGG | 2 cut(s) | 50, 602 |
| BstSFI | CTRYAG | 1 cut(s) | 347 |
| BstV1I | GCAGC | 1 cut(s) | 1028 |
| BstV2I | GAAGAC | 1 cut(s) | 954 |
| BstZ17I | GTATAC | 1 cut(s) | 1077 |
| BsuRI | GGCC | 2 cut(s) | 5, 62 |
| BtsCI | GGATG | 7 cut(s) | 249, 333, 381, 834, 840, 882, 1049 |
| BtsIMutI | CAGTG | 2 cut(s) | 397, 929 |
| Cac8I | GCNNGC | 3 cut(s) | 64, 151, 693 |
| CaiI | CAGNNNCTG | 1 cut(s) | 603 |
| CfoI | GCGC | 1 cut(s) | 155 |
| Cfr13I | GGNCC | 2 cut(s) | 60, 305 |
| CseI | GACGC | 2 cut(s) | 530, 639 |
| Csp6I | GTAC | 3 cut(s) | 114, 268, 748 |
| CviAII | CATG | 9 cut(s) | 145, 368, 413, 428, 578, 751, 790, 800, 1140 |
| CviQI | GTAC | 3 cut(s) | 114, 268, 748 |
| DdeI | CTNAG | 5 cut(s) | 163, 389, 663, 913, 1020 |
| DpnI | GATC | 5 cut(s) | 75, 228, 248, 432, 471 |
| DpnII | GATC | 5 cut(s) | 73, 226, 246, 430, 469 |
| EaeI | YGGCCR | 1 cut(s) | 3 |
| Eam1104I | CTCTTC | 1 cut(s) | 80 |
| EarI | CTCTTC | 1 cut(s) | 80 |
| Ecl136II | GAGCTC | 1 cut(s) | 912 |
| Eco130I | CCWWGG | 3 cut(s) | 238, 674, 1011 |
| Eco24I | GRGCYC | 1 cut(s) | 914 |
| Eco31I | GGTCTC | 1 cut(s) | 830 |
| Eco47I | GGWCC | 1 cut(s) | 305 |
| Eco47III | AGCGCT | 1 cut(s) | 154 |
| Eco53kI | GAGCTC | 1 cut(s) | 912 |
| EcoICRI | GAGCTC | 1 cut(s) | 912 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 55 |
| EcoRII | CCWGG | 2 cut(s) | 50, 602 |
| EcoT14I | CCWWGG | 3 cut(s) | 238, 674, 1011 |
| EcoT38I | GRGCYC | 1 cut(s) | 914 |
| ErhI | CCWWGG | 3 cut(s) | 238, 674, 1011 |
| FaeI | CATG | 9 cut(s) | 148, 371, 416, 431, 581, 754, 793, 803, 1143 |
| FalI | AAGNNNNNCTT | 1 cut(s) | 1139 |
| FaqI | GGGAC | 1 cut(s) | 1090 |
| FatI | CATG | 9 cut(s) | 144, 367, 412, 427, 577, 750, 789, 799, 1139 |
| FauNDI | CATATG | 1 cut(s) | 437 |
| FbaI | TGATCA | 3 cut(s) | 226, 246, 469 |
| FblI | GTMKAC | 1 cut(s) | 1076 |
| Fnu4HI | GCNGC | 1 cut(s) | 1017 |
| FokI | GGATG | 7 cut(s) | 256, 320, 388, 827, 841, 869, 1036 |
| FriOI | GRGCYC | 1 cut(s) | 914 |
| Fsp4HI | GCNGC | 1 cut(s) | 1017 |
| FspBI | CTAG | 4 cut(s) | 20, 588, 713, 887 |
| GlaI | GCGC | 1 cut(s) | 154 |
| GluI | GCNGC | 1 cut(s) | 1017 |
| GsaI | CCCAGC | 1 cut(s) | 139 |
| GsuI | CTGGAG | 1 cut(s) | 1049 |
| HaeII | RGCGCY | 1 cut(s) | 156 |
| HaeIII | GGCC | 2 cut(s) | 5, 62 |
| HgaI | GACGC | 2 cut(s) | 530, 639 |
| HhaI | GCGC | 1 cut(s) | 155 |
| Hin1I | GRCGYC | 1 cut(s) | 631 |
| Hin1II | CATG | 9 cut(s) | 148, 371, 416, 431, 581, 754, 793, 803, 1143 |
| Hin6I | GCGC | 1 cut(s) | 153 |
| HinP1I | GCGC | 1 cut(s) | 153 |
| HincII | GTYRAC | 1 cut(s) | 805 |
| HindII | GTYRAC | 1 cut(s) | 805 |
| HinfI | GANTC | 5 cut(s) | 364, 384, 1024, 1041, 1103 |
| HphI | GGTGA | 1 cut(s) | 619 |
| Hpy166II | GTNNAC | 4 cut(s) | 129, 182, 805, 1077 |
| Hpy188I | TCNGA | 4 cut(s) | 78, 207, 560, 916 |
| Hpy188III | TCNNGA | 6 cut(s) | 103, 308, 381, 450, 545, 1028 |
| Hpy8I | GTNNAC | 4 cut(s) | 129, 182, 805, 1077 |
| Hpy99I | CGWCG | 1 cut(s) | 546 |
| HpyAV | CCTTC | 3 cut(s) | 19, 248, 891 |
| HpyCH4III | ACNGT | 3 cut(s) | 319, 933, 1074 |
| HpyCH4V | TGCA | 5 cut(s) | 349, 457, 593, 671, 1049 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 668 |
| HpyF3I | CTNAG | 5 cut(s) | 163, 389, 663, 913, 1020 |
| Hsp92I | GRCGYC | 1 cut(s) | 631 |
| Hsp92II | CATG | 9 cut(s) | 148, 371, 416, 431, 581, 754, 793, 803, 1143 |
| HspAI | GCGC | 1 cut(s) | 153 |
| Ksp22I | TGATCA | 3 cut(s) | 226, 246, 469 |
| Kzo9I | GATC | 5 cut(s) | 73, 226, 246, 430, 469 |
| LguI | GCTCTTC | 1 cut(s) | 80 |
| LmnI | GCTCC | 2 cut(s) | 404, 909 |
| Lsp1109I | GCAGC | 1 cut(s) | 1028 |
| LweI | GCATC | 5 cut(s) | 342, 530, 819, 888, 1058 |
| MaeI | CTAG | 4 cut(s) | 20, 588, 713, 887 |
| MaeIII | GTNAC | 1 cut(s) | 319 |
| MalI | GATC | 5 cut(s) | 75, 228, 248, 432, 471 |
| MboI | GATC | 5 cut(s) | 73, 226, 246, 430, 469 |
| MboII | GAAGA | 9 cut(s) | 97, 335, 541, 662, 716, 959, 1143, 1156, 1159 |
| MhlI | GDGCHC | 1 cut(s) | 914 |
| MlsI | TGGCCA | 1 cut(s) | 5 |
| MluCI | AATT | 7 cut(s) | 39, 501, 720, 728, 852, 1067, 1092 |
| MluNI | TGGCCA | 1 cut(s) | 5 |
| MlyI | GAGTC | 2 cut(s) | 378, 1112 |
| MmeI | TCCRAC | 1 cut(s) | 230 |
| MnlI | CCTC | 8 cut(s) | 172, 270, 398, 644, 816, 853, 1024, 1121 |
| Mox20I | TGGCCA | 1 cut(s) | 5 |
| MscI | TGGCCA | 1 cut(s) | 5 |
| MseI | TTAA | 5 cut(s) | 108, 158, 339, 554, 1089 |
| MslI | CAYNNNNRTG | 1 cut(s) | 605 |
| Msp20I | TGGCCA | 1 cut(s) | 5 |
| MspA1I | CMGCKG | 1 cut(s) | 1019 |
| MspR9I | CCNGG | 2 cut(s) | 52, 604 |
| Mva1269I | GAATGC | 2 cut(s) | 459, 954 |
| MvaI | CCWGG | 2 cut(s) | 52, 604 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 668 |
| NdeI | CATATG | 1 cut(s) | 437 |
| NdeII | GATC | 5 cut(s) | 73, 226, 246, 430, 469 |
| NlaIII | CATG | 9 cut(s) | 148, 371, 416, 431, 581, 754, 793, 803, 1143 |
| NspI | RCATGY | 1 cut(s) | 803 |
| OliI | CACNNNNGTG | 1 cut(s) | 605 |
| PciSI | GCTCTTC | 1 cut(s) | 80 |
| PctI | GAATGC | 2 cut(s) | 459, 954 |
| PfeI | GAWTC | 3 cut(s) | 364, 1024, 1041 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 603 |
| PfoI | TCCNGGA | 1 cut(s) | 50 |
| PkrI | GCNGC | 1 cut(s) | 1018 |
| PleI | GAGTC | 2 cut(s) | 378, 1111 |
| PpsI | GAGTC | 2 cut(s) | 378, 1111 |
| PshBI | ATTAAT | 1 cut(s) | 108 |
| Psp124BI | GAGCTC | 1 cut(s) | 914 |
| Psp6I | CCWGG | 2 cut(s) | 50, 602 |
| PspFI | CCCAGC | 1 cut(s) | 135 |
| PspGI | CCWGG | 2 cut(s) | 50, 602 |
| PspPI | GGNCC | 2 cut(s) | 60, 305 |
| PstI | CTGCAG | 1 cut(s) | 351 |
| PstNI | CAGNNNCTG | 1 cut(s) | 603 |
| PvuII | CAGCTG | 1 cut(s) | 1019 |
| RsaI | GTAC | 3 cut(s) | 115, 269, 749 |
| RsaNI | GTAC | 3 cut(s) | 114, 268, 748 |
| RseI | CAYNNNNRTG | 1 cut(s) | 605 |
| SacI | GAGCTC | 1 cut(s) | 914 |
| SapI | GCTCTTC | 1 cut(s) | 80 |
| SaqAI | TTAA | 5 cut(s) | 108, 158, 339, 554, 1089 |
| SatI | GCNGC | 1 cut(s) | 1017 |
| Sau3AI | GATC | 5 cut(s) | 73, 226, 246, 430, 469 |
| Sau96I | GGNCC | 2 cut(s) | 60, 305 |
| SchI | GAGTC | 2 cut(s) | 378, 1112 |
| ScrFI | CCNGG | 2 cut(s) | 52, 604 |
| SduI | GDGCHC | 1 cut(s) | 914 |
| SfaNI | GCATC | 5 cut(s) | 342, 530, 819, 888, 1058 |
| SfcI | CTRYAG | 1 cut(s) | 347 |
| SinI | GGWCC | 1 cut(s) | 305 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 605 |
| SmlI | CTYRAG | 1 cut(s) | 833 |
| SmoI | CTYRAG | 1 cut(s) | 833 |
| Sse9I | AATT | 7 cut(s) | 39, 501, 720, 728, 852, 1067, 1092 |
| SspI | AATATT | 1 cut(s) | 202 |
| SspMI | CTAG | 4 cut(s) | 20, 588, 713, 887 |
| SstI | GAGCTC | 1 cut(s) | 914 |
| StyD4I | CCNGG | 2 cut(s) | 50, 602 |
| StyI | CCWWGG | 3 cut(s) | 238, 674, 1011 |
| TaaI | ACNGT | 3 cut(s) | 319, 933, 1074 |
| TaqI | TCGA | 3 cut(s) | 193, 252, 544 |
| TaqII | GACCGA | 1 cut(s) | 293 |
| TasI | AATT | 7 cut(s) | 39, 501, 720, 728, 852, 1067, 1092 |
| TatI | WGTACW | 1 cut(s) | 747 |
| TfiI | GAWTC | 3 cut(s) | 364, 1024, 1041 |
| Tru1I | TTAA | 5 cut(s) | 108, 158, 339, 554, 1089 |
| Tru9I | TTAA | 5 cut(s) | 108, 158, 339, 554, 1089 |
| TscAI | CASTG | 2 cut(s) | 397, 936 |
| TseI | GCWGC | 1 cut(s) | 1016 |
| TspDTI | ATGAA | 6 cut(s) | 312, 437, 629, 767, 806, 960 |
| TspRI | CASTG | 2 cut(s) | 397, 936 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 603 |
| VpaK11BI | GGWCC | 1 cut(s) | 305 |
| VspI | ATTAAT | 1 cut(s) | 108 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 55 |
| XapI | RAATTY | 1 cut(s) | 852 |
| XceI | RCATGY | 1 cut(s) | 803 |
| XmiI | GTMKAC | 1 cut(s) | 1076 |
| XspI | CTAG | 4 cut(s) | 20, 588, 713, 887 |