Rw1G031150

Hsp20/alpha crystallin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000537
Physical Location & Seq
Reverse (-)
59278223 .. 59278699
477 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G031150.1

Sequence Viewer

Length: 477 bp
ATGTCTCTCATTCCGGGAAGCTTTTTCGGCAACGGTCGACGGAGCAACATCTTCGACCCGTTCTCGCTCGACATCTGGGACCCGTTCCAGGACTTCCCCTTGATCAACTCGCGCTCGGCTCCACGCTCGGAGACGGCGGCGGTGGCGAACACGCGCATCGACTGGAAGGAGACGCCGGAGGCGCATGTGTTCAAGGCGGACCTGCCGGGGCTGAAGAAGGAGGAGGTGAAGGTGGAGGTGGAGGAAGGGAAGGTGCTGCAGATCAGCGGCGAGAGGAAAGTGGAGAAGGAGGACAAGAGCGACAAGTGGCACAGGGTGGAGAGGAGCAGCGGTAAGTTCTTGAGGAGGTTCAGGCTGCCGGAGAATGCCAAGGTGGAGCAGGTGAAGGCAGCAATGGAGAATGGGGTGCTTACTGTCACTGTGCCGAAGGTGGAGGTCAAGAAGCCTGATGTGAAGGCCATTCAGATTTCTGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

158

Amino Acids

17.95

Weight (kDa)

8.8

Isoelectric Point (pI)

46.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 54 - 157 1.1e-31 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000537)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07400 AT2G29500
fragaria_vesca FvH4_3g35270 FvH4_3g37330 FvH4_3g37360 FvH4_3g38620 FvH4_7g23650
malus_domestica MD07G1210800.v1.1 MD11G1087100.v1.1 MD11G1088300.v1.1 MD11G1089300.v1.1
prunus_persica Prupe.2G243400_v2.0.a1 Prupe.6G065900_v2.0.a1 Prupe.6G066100_v2.0.a1 Prupe.6G066200_v2.0.a1 Prupe.6G066300_v2.0.a1 Prupe.6G066400_v2.0.a1 Prupe.6G066500_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0368921 RchiOBHm_Chr1g0369001 RchiOBHm_Chr4g0444561 RchiOBHm_Chr5g0063321 RchiOBHm_Chr5g0063331 RchiOBHm_Chr5g0063361 RchiOBHm_Chr5g0067111 RchiOBHm_Chr5g0067191
rosa_laevigata RLG00000005821 RLG00000027165 RLG00000027176 RLG00000035622 RLG00000035623 RLG00000035626 RLG00000035628 RLG00000035914
rosa_multiflora Rmu_co8027810.1_g000001 Rmu_sc0000510.1_g000026 Rmu_sc0003839.1_g000004 Rmu_sc0004250.1_g000031 Rmu_sc0004250.1_g000043 Rmu_sc0005045.1_g000005 Rmu_sc0006795.1_g000002 Rmu_sc0008775.1_g000001 Rmu_sc0008775.1_g000012 Rmu_sc0012648.1_g000004
rosa_roxburghii Rroxscaffold_1G00013950 Rroxscaffold_1G00017380 Rroxscaffold_4G00288090 Rroxscaffold_4G00288200 Rroxscaffold_5G00385020
rosa_rugosa Rorug01G0345900 Rorug01G0346900 Rorug04G0353900 Rorug05G0356000 Rorug05G0356800 Rorug05G0381900 Rorug05G0382600
rosa_samantha Rh1AG353800 Rh1BG317300 Rh1CG331800 Rh1DG347900 Rh1DG348200 Rh4BG425600 Rh4CG440300 Rh4DG421200 Rh5AG415700 Rh5AG415800 Rh5AG416000 Rh5AG441900 Rh5BG430600 Rh5BG430700 Rh5BG431100 Rh5CG454100 Rh5CG454200 Rh5CG454400 Rh5DG444400 Rh5DG444500 Rh5DG444700
rosa_wichuraiana Rw1G031150 Rw1G031260 Rw4G035560 Rw5G039060 Rw5G039080 Rw5G041290 Rw5G041330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 370
Acc36I ACCTGC 2 cut(s) 210, 370
AccI GTMKAC 1 cut(s) 37
AccII CGCG 2 cut(s) 112, 154
AciI CCGC 5 cut(s) 137, 140, 197, 267, 330
AcuI CTGAAG 1 cut(s) 233
AcyI GRCGYC 1 cut(s) 173
AdeI CACNNNGTG 1 cut(s) 316
AfiI CCNNNNNNNGG 1 cut(s) 88
AgsI TTSAA 1 cut(s) 193
AjnI CCWGG 1 cut(s) 87
AluBI AGCT 1 cut(s) 21
AluI AGCT 1 cut(s) 21
Alw26I GTCTC 3 cut(s) 9, 125, 164
AoxI GGCC 1 cut(s) 456
ApeKI GCWGC 4 cut(s) 256, 327, 355, 389
AspLEI GCGC 3 cut(s) 114, 156, 184
AspS9I GGNCC 2 cut(s) 79, 199
AsuC2I CCSGG 2 cut(s) 15, 207
AsuHPI GGTGA 2 cut(s) 238, 394
AvaII GGWCC 2 cut(s) 79, 199
BbvI GCAGC 4 cut(s) 243, 339, 342, 401
BceAI ACGGC 1 cut(s) 150
BcgI CGANNNNNNTGC 2 cut(s) 34, 68
BciT130I CCWGG 1 cut(s) 89
BclI TGATCA 1 cut(s) 102
BcnI CCSGG 2 cut(s) 15, 207
BcoDI GTCTC 3 cut(s) 9, 125, 164
BfaI CTAG 1 cut(s) 475
BfmI CTRYAG 1 cut(s) 257
BfuAI ACCTGC 2 cut(s) 210, 370
BisI GCNGC 6 cut(s) 138, 257, 268, 328, 356, 390
BlsI GCNGC 6 cut(s) 139, 258, 269, 329, 357, 391
Bme1390I CCNGG 3 cut(s) 15, 89, 207
Bme18I GGWCC 2 cut(s) 79, 199
BmgT120I GGNCC 2 cut(s) 79, 199
BmiI GGNNCC 3 cut(s) 80, 81, 120
BmrFI CCNGG 3 cut(s) 15, 89, 207
BmsI GCATC 1 cut(s) 165
BpuEI CTTGAG 1 cut(s) 361
BpuMI CCSGG 2 cut(s) 15, 207
BsaHI GRCGYC 1 cut(s) 173
BsaJI CCNNGG 2 cut(s) 206, 369
BsaXI ACNNNNNCTCC 2 cut(s) 316, 346
Bsc4I CCNNNNNNNGG 1 cut(s) 88
Bse1I ACTGG 1 cut(s) 167
Bse3DI GCAATG 1 cut(s) 399
BseBI CCWGG 1 cut(s) 89
BseDI CCNNGG 2 cut(s) 206, 369
BseLI CCNNNNNNNGG 1 cut(s) 88
BseMI GCAATG 1 cut(s) 399
BseNI ACTGG 1 cut(s) 167
BseRI GAGGAG 3 cut(s) 236, 337, 358
BseXI GCAGC 4 cut(s) 243, 339, 342, 401
Bsh1236I CGCG 2 cut(s) 112, 154
Bsh1285I CGRYCG 1 cut(s) 37
BshFI GGCC 1 cut(s) 458
BsiEI CGRYCG 1 cut(s) 37
BsiSI CCGG 4 cut(s) 14, 176, 206, 359
BslFI GGGAC 1 cut(s) 92
BslI CCNNNNNNNGG 1 cut(s) 88
BsmAI GTCTC 3 cut(s) 9, 125, 164
BsmBI CGTCTC 2 cut(s) 125, 164
BsmFI GGGAC 1 cut(s) 92
BsmI GAATGC 1 cut(s) 370
BsnI GGCC 1 cut(s) 458
Bsp143I GATC 2 cut(s) 102, 261
BspACI CCGC 5 cut(s) 137, 140, 197, 267, 330
BspANI GGCC 1 cut(s) 458
BspFNI CGCG 2 cut(s) 112, 154
BspLI GGNNCC 3 cut(s) 80, 81, 120
BspMAI CTGCAG 1 cut(s) 261
BspMI ACCTGC 2 cut(s) 210, 370
BsrDI GCAATG 1 cut(s) 399
BsrI ACTGG 1 cut(s) 167
BssECI CCNNGG 2 cut(s) 206, 369
BssMI GATC 2 cut(s) 102, 261
BssNI GRCGYC 1 cut(s) 173
BssT1I CCWWGG 1 cut(s) 369
Bst2UI CCWGG 1 cut(s) 89
Bst4CI ACNGT 3 cut(s) 35, 415, 421
BstACI GRCGYC 1 cut(s) 173
BstFNI CGCG 2 cut(s) 112, 154
BstHHI GCGC 3 cut(s) 114, 156, 184
BstKTI GATC 2 cut(s) 105, 264
BstMAI GTCTC 3 cut(s) 9, 125, 164
BstMBI GATC 2 cut(s) 102, 261
BstMCI CGRYCG 1 cut(s) 37
BstMWI GCNNNNNNNGC 3 cut(s) 27, 143, 181
BstNI CCWGG 1 cut(s) 89
BstNSI RCATGY 1 cut(s) 188
BstSCI CCNGG 3 cut(s) 13, 87, 205
BstSFI CTRYAG 1 cut(s) 257
BstUI CGCG 2 cut(s) 112, 154
BstV1I GCAGC 4 cut(s) 243, 339, 342, 401
BsuRI GGCC 1 cut(s) 458
BtsIMutI CAGTG 1 cut(s) 417
BveI ACCTGC 2 cut(s) 210, 370
CfoI GCGC 3 cut(s) 114, 156, 184
Cfr13I GGNCC 2 cut(s) 79, 199
CseI GACGC 1 cut(s) 181
CviAII CATG 1 cut(s) 185
CviJI RGCY 6 cut(s) 21, 119, 211, 355, 445, 458
CviKI_1 RGCY 6 cut(s) 21, 119, 211, 355, 445, 458
DpnI GATC 2 cut(s) 104, 263
DpnII GATC 2 cut(s) 102, 261
DraIII CACNNNGTG 1 cut(s) 316
EciI GGCGGA 1 cut(s) 212
Eco130I CCWWGG 1 cut(s) 369
Eco47I GGWCC 2 cut(s) 79, 199
Eco57I CTGAAG 1 cut(s) 233
EcoO109I RGGNCCY 1 cut(s) 79
EcoRII CCWGG 1 cut(s) 87
EcoT14I CCWWGG 1 cut(s) 369
ErhI CCWWGG 1 cut(s) 369
Esp3I CGTCTC 2 cut(s) 125, 164
FaeI CATG 1 cut(s) 188
FaiI YATR 1 cut(s) 186
FaqI GGGAC 1 cut(s) 92
FatI CATG 1 cut(s) 184
FbaI TGATCA 1 cut(s) 102
FblI GTMKAC 1 cut(s) 37
Fnu4HI GCNGC 6 cut(s) 138, 257, 268, 328, 356, 390
Fsp4HI GCNGC 6 cut(s) 138, 257, 268, 328, 356, 390
FspBI CTAG 1 cut(s) 475
GlaI GCGC 3 cut(s) 113, 155, 183
GluI GCNGC 6 cut(s) 138, 257, 268, 328, 356, 390
HaeIII GGCC 1 cut(s) 458
HapII CCGG 4 cut(s) 14, 176, 206, 359
HgaI GACGC 1 cut(s) 181
HhaI GCGC 3 cut(s) 114, 156, 184
Hin1I GRCGYC 1 cut(s) 173
Hin1II CATG 1 cut(s) 188
Hin6I GCGC 3 cut(s) 112, 154, 182
HinP1I GCGC 3 cut(s) 112, 154, 182
HincII GTYRAC 1 cut(s) 38
HindII GTYRAC 1 cut(s) 38
HindIII AAGCTT 1 cut(s) 19
HpaII CCGG 4 cut(s) 14, 176, 206, 359
HphI GGTGA 2 cut(s) 238, 394
Hpy166II GTNNAC 1 cut(s) 38
Hpy188I TCNGA 3 cut(s) 130, 465, 472
Hpy188III TCNNGA 2 cut(s) 340, 439
Hpy8I GTNNAC 1 cut(s) 38
Hpy99I CGWCG 1 cut(s) 42
HpyAV CCTTC 9 cut(s) 160, 211, 223, 239, 244, 280, 379, 421, 448
HpyCH4III ACNGT 3 cut(s) 35, 415, 421
HpyCH4V TGCA 1 cut(s) 259
HpyF10VI GCNNNNNNNGC 3 cut(s) 27, 143, 181
Hsp92I GRCGYC 1 cut(s) 173
Hsp92II CATG 1 cut(s) 188
HspAI GCGC 3 cut(s) 112, 154, 182
KflI GGGWCCC 1 cut(s) 79
Ksp22I TGATCA 1 cut(s) 102
Kzo9I GATC 2 cut(s) 102, 261
LmnI GCTCC 4 cut(s) 42, 124, 324, 376
Lsp1109I GCAGC 4 cut(s) 243, 339, 342, 401
LweI GCATC 1 cut(s) 165
MaeI CTAG 1 cut(s) 475
MaeIII GTNAC 1 cut(s) 415
MalI GATC 2 cut(s) 104, 263
MboI GATC 2 cut(s) 102, 261
MboII GAAGA 2 cut(s) 43, 226
MspA1I CMGCKG 2 cut(s) 267, 330
MspI CCGG 4 cut(s) 14, 176, 206, 359
MspR9I CCNGG 3 cut(s) 15, 89, 207
Mva1269I GAATGC 1 cut(s) 370
MvaI CCWGG 1 cut(s) 89
MvnI CGCG 2 cut(s) 112, 154
MwoI GCNNNNNNNGC 3 cut(s) 27, 143, 181
NciI CCSGG 2 cut(s) 15, 207
NdeII GATC 2 cut(s) 102, 261
NlaIII CATG 1 cut(s) 188
NlaIV GGNNCC 3 cut(s) 80, 81, 120
NmeAIII GCCGAG 1 cut(s) 95
NmuCI GTSAC 1 cut(s) 415
NspI RCATGY 1 cut(s) 188
PaqCI CACCTGC 1 cut(s) 370
PctI GAATGC 1 cut(s) 370
PfoI TCCNGGA 2 cut(s) 13, 87
PkrI GCNGC 6 cut(s) 139, 258, 269, 329, 357, 391
PpuMI RGGWCCY 1 cut(s) 79
Psp5II RGGWCCY 1 cut(s) 79
Psp6I CCWGG 1 cut(s) 87
PspGI CCWGG 1 cut(s) 87
PspN4I GGNNCC 3 cut(s) 80, 81, 120
PspPI GGNCC 2 cut(s) 79, 199
PspPPI RGGWCCY 1 cut(s) 79
PstI CTGCAG 1 cut(s) 261
SalI GTCGAC 1 cut(s) 36
SatI GCNGC 6 cut(s) 138, 257, 268, 328, 356, 390
Sau3AI GATC 2 cut(s) 102, 261
Sau96I GGNCC 2 cut(s) 79, 199
ScrFI CCNGG 3 cut(s) 15, 89, 207
SfaNI GCATC 1 cut(s) 165
SfcI CTRYAG 1 cut(s) 257
SinI GGWCC 2 cut(s) 79, 199
SmlI CTYRAG 1 cut(s) 340
SmoI CTYRAG 1 cut(s) 340
SsiI CCGC 5 cut(s) 137, 140, 197, 267, 330
SspMI CTAG 1 cut(s) 475
StyD4I CCNGG 3 cut(s) 13, 87, 205
StyI CCWWGG 1 cut(s) 369
TaaI ACNGT 3 cut(s) 35, 415, 421
TaqI TCGA 4 cut(s) 37, 54, 69, 159
TauI GCSGC 2 cut(s) 140, 270
TscAI CASTG 1 cut(s) 424
TseFI GTSAC 1 cut(s) 415
TseI GCWGC 4 cut(s) 256, 327, 355, 389
Tsp45I GTSAC 1 cut(s) 415
TspGWI ACGGA 1 cut(s) 55
TspRI CASTG 1 cut(s) 424
VpaK11BI GGWCC 2 cut(s) 79, 199
XceI RCATGY 1 cut(s) 188
XmiI GTMKAC 1 cut(s) 37
XspI CTAG 1 cut(s) 475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.