RchiOBHm_Chr5g0067111

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000537
Physical Location & Seq
Reverse (-)
73165407 .. 73166281
875 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34284

Sequence Viewer

Length: 435 bp
ATGTCGCTTATCCCCAATTTCCGACGTAGCACGGATTTTCCATTCCCTTCTTCATCGCTCTCTTTTTTTCCCGAATTTCCTCGCGAAAATTCGGCTTTTGTCAACACTAGGATCGACTGGAAGGAGACCCCGGAAGCCCATGTTTTCAAGGCTGACATTCCGGGGCTGACGAAAGAAGAGATCAAGGTTGAGGTAGAAGACGACAGGGTGCTTCAGATCAGCGGAGAGAGGAAGATAGAGAATAAGGAGGACAAGAACGGCACCTGGCACCGGGTCGAGAGAAGCAGCGGCAAGTTCTCCAGAAGGTTCAGGCTTCCTGAGAATGCCAAGATGGATGAAATCAAGGCTGCTATGGAGAACGGGGTTCTCAGCGTGACTGTTCCAAAGGCGGAGGTGAAAAGGCCTGATGTTAAAGCCATTCAAATCTCTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

144

Amino Acids

16.52

Weight (kDa)

7.97

Isoelectric Point (pI)

53.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 39 - 143 6.2e-32 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000537)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07400 AT2G29500
fragaria_vesca FvH4_3g35270 FvH4_3g37330 FvH4_3g37360 FvH4_3g38620 FvH4_7g23650
malus_domestica MD07G1210800.v1.1 MD11G1087100.v1.1 MD11G1088300.v1.1 MD11G1089300.v1.1
prunus_persica Prupe.2G243400_v2.0.a1 Prupe.6G065900_v2.0.a1 Prupe.6G066100_v2.0.a1 Prupe.6G066200_v2.0.a1 Prupe.6G066300_v2.0.a1 Prupe.6G066400_v2.0.a1 Prupe.6G066500_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0368921 RchiOBHm_Chr1g0369001 RchiOBHm_Chr4g0444561 RchiOBHm_Chr5g0063321 RchiOBHm_Chr5g0063331 RchiOBHm_Chr5g0063361 RchiOBHm_Chr5g0067111 RchiOBHm_Chr5g0067191
rosa_laevigata RLG00000005821 RLG00000027165 RLG00000027176 RLG00000035622 RLG00000035623 RLG00000035626 RLG00000035628 RLG00000035914
rosa_multiflora Rmu_co8027810.1_g000001 Rmu_sc0000510.1_g000026 Rmu_sc0003839.1_g000004 Rmu_sc0004250.1_g000031 Rmu_sc0004250.1_g000043 Rmu_sc0005045.1_g000005 Rmu_sc0006795.1_g000002 Rmu_sc0008775.1_g000001 Rmu_sc0008775.1_g000012 Rmu_sc0012648.1_g000004
rosa_roxburghii Rroxscaffold_1G00013950 Rroxscaffold_1G00017380 Rroxscaffold_4G00288090 Rroxscaffold_4G00288200 Rroxscaffold_5G00385020
rosa_rugosa Rorug01G0345900 Rorug01G0346900 Rorug04G0353900 Rorug05G0356000 Rorug05G0356800 Rorug05G0381900 Rorug05G0382600
rosa_samantha Rh1AG353800 Rh1BG317300 Rh1CG331800 Rh1DG347900 Rh1DG348200 Rh4BG425600 Rh4CG440300 Rh4DG421200 Rh5AG415700 Rh5AG415800 Rh5AG416000 Rh5AG441900 Rh5BG430600 Rh5BG430700 Rh5BG431100 Rh5CG454100 Rh5CG454200 Rh5CG454400 Rh5DG444400 Rh5DG444500 Rh5DG444700
rosa_wichuraiana Rw1G031150 Rw1G031260 Rw4G035560 Rw5G039060 Rw5G039080 Rw5G041290 Rw5G041330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 260, 267
AccII CGCG 1 cut(s) 84
AciI CCGC 3 cut(s) 222, 288, 389
AclWI GGATC 1 cut(s) 119
AcsI RAATTY 2 cut(s) 74, 88
AcuI CTGAAG 1 cut(s) 197
AfiI CCNNNNNNNGG 1 cut(s) 270
AgsI TTSAA 2 cut(s) 148, 422
AjnI CCWGG 1 cut(s) 263
Alw26I GTCTC 1 cut(s) 119
AlwI GGATC 1 cut(s) 119
AoxI GGCC 1 cut(s) 401
ApeKI GCWGC 2 cut(s) 285, 347
ApoI RAATTY 2 cut(s) 74, 88
AsuC2I CCSGG 3 cut(s) 131, 162, 272
AsuHPI GGTGA 1 cut(s) 406
BanI GGYRCC 2 cut(s) 260, 267
BbsI GAAGAC 1 cut(s) 204
BbvI GCAGC 2 cut(s) 297, 334
BccI CCATC 1 cut(s) 325
BceAI ACGGC 1 cut(s) 274
BciT130I CCWGG 1 cut(s) 265
BcnI CCSGG 3 cut(s) 131, 162, 272
BcoDI GTCTC 1 cut(s) 119
BfaI CTAG 1 cut(s) 108
BisI GCNGC 3 cut(s) 286, 289, 348
BlsI GCNGC 3 cut(s) 287, 290, 349
Bme1390I CCNGG 4 cut(s) 131, 162, 265, 272
BmiI GGNNCC 2 cut(s) 262, 269
BmrFI CCNGG 4 cut(s) 131, 162, 265, 272
BpiI GAAGAC 1 cut(s) 204
BpmI CTGGAG 1 cut(s) 283
BpuMI CCSGG 3 cut(s) 131, 162, 272
BsaI GGTCTC 1 cut(s) 119
BsaJI CCNNGG 2 cut(s) 129, 161
Bsc4I CCNNNNNNNGG 1 cut(s) 270
Bse1I ACTGG 1 cut(s) 122
BseBI CCWGG 1 cut(s) 265
BseDI CCNNGG 2 cut(s) 129, 161
BseGI GGATG 1 cut(s) 340
BseLI CCNNNNNNNGG 1 cut(s) 270
BseMII CTCAG 2 cut(s) 309, 382
BseNI ACTGG 1 cut(s) 122
BseXI GCAGC 2 cut(s) 297, 334
Bsh1236I CGCG 1 cut(s) 84
BshFI GGCC 1 cut(s) 403
BshNI GGYRCC 2 cut(s) 260, 267
BsiSI CCGG 3 cut(s) 131, 161, 271
BslI CCNNNNNNNGG 1 cut(s) 270
BsmAI GTCTC 1 cut(s) 119
BsmI GAATGC 1 cut(s) 328
BsnI GGCC 1 cut(s) 403
Bso31I GGTCTC 1 cut(s) 119
Bsp143I GATC 3 cut(s) 111, 180, 216
Bsp68I TCGCGA 1 cut(s) 84
BspACI CCGC 3 cut(s) 222, 288, 389
BspANI GGCC 1 cut(s) 403
BspCNI CTCAG 2 cut(s) 310, 381
BspFNI CGCG 1 cut(s) 84
BspLI GGNNCC 2 cut(s) 262, 269
BspPI GGATC 1 cut(s) 119
BspT107I GGYRCC 2 cut(s) 260, 267
BspTNI GGTCTC 1 cut(s) 119
BsrI ACTGG 1 cut(s) 122
BssECI CCNNGG 2 cut(s) 129, 161
BssMI GATC 3 cut(s) 111, 180, 216
Bst2UI CCWGG 1 cut(s) 265
Bst4CI ACNGT 1 cut(s) 379
Bst6I CTCTTC 1 cut(s) 171
BstDEI CTNAG 2 cut(s) 318, 368
BstF5I GGATG 1 cut(s) 340
BstFNI CGCG 1 cut(s) 84
BstKTI GATC 3 cut(s) 114, 183, 219
BstMAI GTCTC 1 cut(s) 119
BstMBI GATC 3 cut(s) 111, 180, 216
BstNI CCWGG 1 cut(s) 265
BstSCI CCNGG 4 cut(s) 129, 160, 263, 270
BstUI CGCG 1 cut(s) 84
BstV1I GCAGC 2 cut(s) 297, 334
BstV2I GAAGAC 1 cut(s) 204
BsuRI GGCC 1 cut(s) 403
BtgZI GCGATG 1 cut(s) 39
BtsCI GGATG 1 cut(s) 340
BtuMI TCGCGA 1 cut(s) 84
CviAII CATG 1 cut(s) 140
CviJI RGCY 8 cut(s) 95, 137, 152, 166, 313, 347, 403, 416
CviKI_1 RGCY 8 cut(s) 95, 137, 152, 166, 313, 347, 403, 416
DdeI CTNAG 2 cut(s) 318, 368
DpnI GATC 3 cut(s) 113, 182, 218
DpnII GATC 3 cut(s) 111, 180, 216
Eam1104I CTCTTC 1 cut(s) 171
EarI CTCTTC 1 cut(s) 171
EciI GGCGGA 1 cut(s) 404
Eco147I AGGCCT 1 cut(s) 403
Eco31I GGTCTC 1 cut(s) 119
Eco57I CTGAAG 1 cut(s) 197
EcoRII CCWGG 1 cut(s) 263
FaeI CATG 1 cut(s) 143
FaiI YATR 2 cut(s) 141, 353
FatI CATG 1 cut(s) 139
Fnu4HI GCNGC 3 cut(s) 286, 289, 348
FokI GGATG 1 cut(s) 347
Fsp4HI GCNGC 3 cut(s) 286, 289, 348
FspBI CTAG 1 cut(s) 108
GluI GCNGC 3 cut(s) 286, 289, 348
GsuI CTGGAG 1 cut(s) 283
HaeIII GGCC 1 cut(s) 403
HapII CCGG 3 cut(s) 131, 161, 271
Hin1II CATG 1 cut(s) 143
HincII GTYRAC 1 cut(s) 103
HindII GTYRAC 1 cut(s) 103
HpaII CCGG 3 cut(s) 131, 161, 271
HphI GGTGA 1 cut(s) 406
Hpy166II GTNNAC 1 cut(s) 103
Hpy188I TCNGA 2 cut(s) 23, 216
Hpy188III TCNNGA 5 cut(s) 71, 83, 277, 300, 317
Hpy8I GTNNAC 1 cut(s) 103
Hpy99I CGWCG 1 cut(s) 27
HpyAV CCTTC 3 cut(s) 57, 115, 297
HpyCH4III ACNGT 1 cut(s) 379
HpyCH4IV ACGT 1 cut(s) 25
HpyF3I CTNAG 2 cut(s) 318, 368
HpySE526I ACGT 1 cut(s) 25
Hsp92II CATG 1 cut(s) 143
Kzo9I GATC 3 cut(s) 111, 180, 216
Lsp1109I GCAGC 2 cut(s) 297, 334
MaeI CTAG 1 cut(s) 108
MaeII ACGT 1 cut(s) 25
MaeIII GTNAC 1 cut(s) 373
MalI GATC 3 cut(s) 113, 182, 218
MboI GATC 3 cut(s) 111, 180, 216
MboII GAAGA 4 cut(s) 42, 188, 209, 244
MluCI AATT 3 cut(s) 16, 74, 88
MmeI TCCRAC 1 cut(s) 46
MnlI CCTC 5 cut(s) 90, 184, 222, 241, 385
MseI TTAA 2 cut(s) 411, 433
MspA1I CMGCKG 2 cut(s) 222, 288
MspI CCGG 3 cut(s) 131, 161, 271
MspR9I CCNGG 4 cut(s) 131, 162, 265, 272
Mva1269I GAATGC 1 cut(s) 328
MvaI CCWGG 1 cut(s) 265
MvnI CGCG 1 cut(s) 84
NciI CCSGG 3 cut(s) 131, 162, 272
NdeII GATC 3 cut(s) 111, 180, 216
NlaIII CATG 1 cut(s) 143
NlaIV GGNNCC 2 cut(s) 262, 269
NmuCI GTSAC 1 cut(s) 373
NruI TCGCGA 1 cut(s) 84
PceI AGGCCT 1 cut(s) 403
PctI GAATGC 1 cut(s) 328
PkrI GCNGC 3 cut(s) 287, 290, 349
Psp6I CCWGG 1 cut(s) 263
PspGI CCWGG 1 cut(s) 263
PspN4I GGNNCC 2 cut(s) 262, 269
RruI TCGCGA 1 cut(s) 84
SaqAI TTAA 2 cut(s) 411, 433
SatI GCNGC 3 cut(s) 286, 289, 348
Sau3AI GATC 3 cut(s) 111, 180, 216
ScrFI CCNGG 4 cut(s) 131, 162, 265, 272
SetI ASST 6 cut(s) 28, 189, 195, 266, 308, 396
Sse9I AATT 3 cut(s) 16, 74, 88
SseBI AGGCCT 1 cut(s) 403
SsiI CCGC 3 cut(s) 222, 288, 389
SspMI CTAG 1 cut(s) 108
StuI AGGCCT 1 cut(s) 403
StyD4I CCNGG 4 cut(s) 129, 160, 263, 270
TaaI ACNGT 1 cut(s) 379
TaiI ACGT 1 cut(s) 28
TaqI TCGA 2 cut(s) 114, 276
TasI AATT 3 cut(s) 16, 74, 88
TauI GCSGC 1 cut(s) 291
Tru1I TTAA 2 cut(s) 411, 433
Tru9I TTAA 2 cut(s) 411, 433
TseFI GTSAC 1 cut(s) 373
TseI GCWGC 2 cut(s) 285, 347
Tsp45I GTSAC 1 cut(s) 373
TspDTI ATGAA 2 cut(s) 42, 351
TspGWI ACGGA 1 cut(s) 47
XapI RAATTY 2 cut(s) 74, 88
XspI CTAG 1 cut(s) 108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.