RchiOBHm_Chr4g0444561

Hsp20/alpha crystallin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000537
Physical Location & Seq
Forward (+)
65247038 .. 65247950
913 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41220

Sequence Viewer

Length: 366 bp
ATGTCTCTCATTCCGGGAAGCTTTTTCGGCAACGGTCGACGGAGTAACATCTTCGACCCGTTCTCGCTGGACATCTGGGACCCGTTCCAGGACTTCCCCTTGATCAACTCCCGCTGGGCTCCAAGCTCAGAGACGGCGGCGGTGGCGAACACGCGCATCGACTGGAAGGAGACGCCGGAGGCGCACGTCGACAAGTGGCACAGGCTGGAGAGGAGCAGCGGCAAGTTCTTGAGGAGGTTCAGGCTGCCGGAGAATGCCAAGGTGGAGCAGGTGAATGCGGCAATGGAGAATGGGGTGCTTACTGTCACTGTGCCAAAGGTGGAGGTCAAGAAGCCTGATGTGAAGGCCATTCAGATTTCTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

121

Amino Acids

13.8

Weight (kDa)

8.08

Isoelectric Point (pI)

52.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 59 - 120 8.6e-18 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000537)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07400 AT2G29500
fragaria_vesca FvH4_3g35270 FvH4_3g37330 FvH4_3g37360 FvH4_3g38620 FvH4_7g23650
malus_domestica MD07G1210800.v1.1 MD11G1087100.v1.1 MD11G1088300.v1.1 MD11G1089300.v1.1
prunus_persica Prupe.2G243400_v2.0.a1 Prupe.6G065900_v2.0.a1 Prupe.6G066100_v2.0.a1 Prupe.6G066200_v2.0.a1 Prupe.6G066300_v2.0.a1 Prupe.6G066400_v2.0.a1 Prupe.6G066500_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0368921 RchiOBHm_Chr1g0369001 RchiOBHm_Chr4g0444561 RchiOBHm_Chr5g0063321 RchiOBHm_Chr5g0063331 RchiOBHm_Chr5g0063361 RchiOBHm_Chr5g0067111 RchiOBHm_Chr5g0067191
rosa_laevigata RLG00000005821 RLG00000027165 RLG00000027176 RLG00000035622 RLG00000035623 RLG00000035626 RLG00000035628 RLG00000035914
rosa_multiflora Rmu_co8027810.1_g000001 Rmu_sc0000510.1_g000026 Rmu_sc0003839.1_g000004 Rmu_sc0004250.1_g000031 Rmu_sc0004250.1_g000043 Rmu_sc0005045.1_g000005 Rmu_sc0006795.1_g000002 Rmu_sc0008775.1_g000001 Rmu_sc0008775.1_g000012 Rmu_sc0012648.1_g000004
rosa_roxburghii Rroxscaffold_1G00013950 Rroxscaffold_1G00017380 Rroxscaffold_4G00288090 Rroxscaffold_4G00288200 Rroxscaffold_5G00385020
rosa_rugosa Rorug01G0345900 Rorug01G0346900 Rorug04G0353900 Rorug05G0356000 Rorug05G0356800 Rorug05G0381900 Rorug05G0382600
rosa_samantha Rh1AG353800 Rh1BG317300 Rh1CG331800 Rh1DG347900 Rh1DG348200 Rh4BG425600 Rh4CG440300 Rh4DG421200 Rh5AG415700 Rh5AG415800 Rh5AG416000 Rh5AG441900 Rh5BG430600 Rh5BG430700 Rh5BG431100 Rh5CG454100 Rh5CG454200 Rh5CG454400 Rh5DG444400 Rh5DG444500 Rh5DG444700
rosa_wichuraiana Rw1G031150 Rw1G031260 Rw4G035560 Rw5G039060 Rw5G039080 Rw5G041290 Rw5G041330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 259
Acc36I ACCTGC 1 cut(s) 259
AccI GTMKAC 2 cut(s) 37, 189
AccII CGCG 1 cut(s) 154
AciI CCGC 5 cut(s) 112, 137, 140, 219, 278
AcyI GRCGYC 1 cut(s) 173
AfiI CCNNNNNNNGG 1 cut(s) 88
AjiI CACGTC 1 cut(s) 187
AjnI CCWGG 1 cut(s) 87
AluBI AGCT 2 cut(s) 21, 126
AluI AGCT 2 cut(s) 21, 126
Alw26I GTCTC 3 cut(s) 9, 125, 164
AoxI GGCC 1 cut(s) 345
ApeKI GCWGC 2 cut(s) 216, 244
AspLEI GCGC 2 cut(s) 156, 184
AspS9I GGNCC 1 cut(s) 79
AsuC2I CCSGG 1 cut(s) 15
AsuHPI GGTGA 1 cut(s) 283
AvaII GGWCC 1 cut(s) 79
BanII GRGCYC 1 cut(s) 121
BbvI GCAGC 2 cut(s) 228, 231
BceAI ACGGC 1 cut(s) 150
BciT130I CCWGG 1 cut(s) 89
BclI TGATCA 1 cut(s) 102
BcnI CCSGG 1 cut(s) 15
BcoDI GTCTC 3 cut(s) 9, 125, 164
BfuAI ACCTGC 1 cut(s) 259
BisI GCNGC 5 cut(s) 138, 217, 220, 245, 279
BlsI GCNGC 5 cut(s) 139, 218, 221, 246, 280
Bme1390I CCNGG 2 cut(s) 15, 89
Bme18I GGWCC 1 cut(s) 79
BmgBI CACGTC 1 cut(s) 187
BmgT120I GGNCC 1 cut(s) 79
BmiI GGNNCC 3 cut(s) 80, 81, 120
BmrFI CCNGG 2 cut(s) 15, 89
BmsI GCATC 1 cut(s) 165
BpmI CTGGAG 1 cut(s) 227
BpuEI CTTGAG 1 cut(s) 250
BpuMI CCSGG 1 cut(s) 15
BsaHI GRCGYC 1 cut(s) 173
BsaJI CCNNGG 1 cut(s) 258
Bsc4I CCNNNNNNNGG 1 cut(s) 88
Bse1I ACTGG 1 cut(s) 167
Bse3DI GCAATG 1 cut(s) 288
BseBI CCWGG 1 cut(s) 89
BseDI CCNNGG 1 cut(s) 258
BseLI CCNNNNNNNGG 1 cut(s) 88
BseMI GCAATG 1 cut(s) 288
BseMII CTCAG 1 cut(s) 141
BseNI ACTGG 1 cut(s) 167
BseRI GAGGAG 2 cut(s) 226, 247
BseXI GCAGC 2 cut(s) 228, 231
BseYI CCCAGC 1 cut(s) 114
Bsh1236I CGCG 1 cut(s) 154
Bsh1285I CGRYCG 1 cut(s) 37
BshFI GGCC 1 cut(s) 347
BsiEI CGRYCG 1 cut(s) 37
BsiSI CCGG 3 cut(s) 14, 176, 248
BslFI GGGAC 1 cut(s) 92
BslI CCNNNNNNNGG 1 cut(s) 88
BsmAI GTCTC 3 cut(s) 9, 125, 164
BsmBI CGTCTC 2 cut(s) 125, 164
BsmFI GGGAC 1 cut(s) 92
BsmI GAATGC 2 cut(s) 259, 280
BsnI GGCC 1 cut(s) 347
Bsp1286I GDGCHC 1 cut(s) 121
Bsp143I GATC 1 cut(s) 102
BspACI CCGC 5 cut(s) 112, 137, 140, 219, 278
BspANI GGCC 1 cut(s) 347
BspCNI CTCAG 1 cut(s) 140
BspFNI CGCG 1 cut(s) 154
BspLI GGNNCC 3 cut(s) 80, 81, 120
BspMI ACCTGC 1 cut(s) 259
BsrDI GCAATG 1 cut(s) 288
BsrI ACTGG 1 cut(s) 167
BssECI CCNNGG 1 cut(s) 258
BssMI GATC 1 cut(s) 102
BssNI GRCGYC 1 cut(s) 173
BssT1I CCWWGG 1 cut(s) 258
Bst2UI CCWGG 1 cut(s) 89
Bst4CI ACNGT 3 cut(s) 35, 304, 310
BstACI GRCGYC 1 cut(s) 173
BstDEI CTNAG 1 cut(s) 127
BstFNI CGCG 1 cut(s) 154
BstHHI GCGC 2 cut(s) 156, 184
BstKTI GATC 1 cut(s) 105
BstMAI GTCTC 3 cut(s) 9, 125, 164
BstMBI GATC 1 cut(s) 102
BstMCI CGRYCG 1 cut(s) 37
BstMWI GCNNNNNNNGC 3 cut(s) 27, 143, 181
BstNI CCWGG 1 cut(s) 89
BstSCI CCNGG 2 cut(s) 13, 87
BstUI CGCG 1 cut(s) 154
BstV1I GCAGC 2 cut(s) 228, 231
BsuRI GGCC 1 cut(s) 347
BtrI CACGTC 1 cut(s) 187
BtsIMutI CAGTG 1 cut(s) 306
BveI ACCTGC 1 cut(s) 259
CfoI GCGC 2 cut(s) 156, 184
Cfr13I GGNCC 1 cut(s) 79
CseI GACGC 1 cut(s) 181
CviJI RGCY 7 cut(s) 21, 119, 126, 205, 244, 334, 347
CviKI_1 RGCY 7 cut(s) 21, 119, 126, 205, 244, 334, 347
DdeI CTNAG 1 cut(s) 127
DpnI GATC 1 cut(s) 104
DpnII GATC 1 cut(s) 102
Eco130I CCWWGG 1 cut(s) 258
Eco24I GRGCYC 1 cut(s) 121
Eco47I GGWCC 1 cut(s) 79
EcoO109I RGGNCCY 1 cut(s) 79
EcoRII CCWGG 1 cut(s) 87
EcoT14I CCWWGG 1 cut(s) 258
EcoT38I GRGCYC 1 cut(s) 121
ErhI CCWWGG 1 cut(s) 258
Esp3I CGTCTC 2 cut(s) 125, 164
FaqI GGGAC 1 cut(s) 92
FauI CCCGC 1 cut(s) 119
FbaI TGATCA 1 cut(s) 102
FblI GTMKAC 2 cut(s) 37, 189
Fnu4HI GCNGC 5 cut(s) 138, 217, 220, 245, 279
FriOI GRGCYC 1 cut(s) 121
Fsp4HI GCNGC 5 cut(s) 138, 217, 220, 245, 279
GlaI GCGC 2 cut(s) 155, 183
GluI GCNGC 5 cut(s) 138, 217, 220, 245, 279
GsaI CCCAGC 1 cut(s) 118
GsuI CTGGAG 1 cut(s) 227
HaeIII GGCC 1 cut(s) 347
HapII CCGG 3 cut(s) 14, 176, 248
HgaI GACGC 1 cut(s) 181
HhaI GCGC 2 cut(s) 156, 184
Hin1I GRCGYC 1 cut(s) 173
Hin6I GCGC 2 cut(s) 154, 182
HinP1I GCGC 2 cut(s) 154, 182
HincII GTYRAC 2 cut(s) 38, 190
HindII GTYRAC 2 cut(s) 38, 190
HindIII AAGCTT 1 cut(s) 19
HpaII CCGG 3 cut(s) 14, 176, 248
HphI GGTGA 1 cut(s) 283
Hpy166II GTNNAC 2 cut(s) 38, 190
Hpy188I TCNGA 3 cut(s) 130, 354, 361
Hpy188III TCNNGA 2 cut(s) 229, 328
Hpy8I GTNNAC 2 cut(s) 38, 190
Hpy99I CGWCG 2 cut(s) 42, 191
HpyAV CCTTC 2 cut(s) 160, 337
HpyCH4III ACNGT 3 cut(s) 35, 304, 310
HpyCH4IV ACGT 1 cut(s) 186
HpyF10VI GCNNNNNNNGC 3 cut(s) 27, 143, 181
HpyF3I CTNAG 1 cut(s) 127
HpySE526I ACGT 1 cut(s) 186
Hsp92I GRCGYC 1 cut(s) 173
HspAI GCGC 2 cut(s) 154, 182
KflI GGGWCCC 1 cut(s) 79
Ksp22I TGATCA 1 cut(s) 102
Kzo9I GATC 1 cut(s) 102
LmnI GCTCC 3 cut(s) 124, 213, 265
Lsp1109I GCAGC 2 cut(s) 228, 231
LweI GCATC 1 cut(s) 165
MaeII ACGT 1 cut(s) 186
MaeIII GTNAC 2 cut(s) 44, 304
MalI GATC 1 cut(s) 104
MboI GATC 1 cut(s) 102
MboII GAAGA 1 cut(s) 43
MhlI GDGCHC 1 cut(s) 121
MnlI CCTC 5 cut(s) 172, 204, 225, 228, 316
MspA1I CMGCKG 2 cut(s) 114, 219
MspI CCGG 3 cut(s) 14, 176, 248
MspR9I CCNGG 2 cut(s) 15, 89
Mva1269I GAATGC 2 cut(s) 259, 280
MvaI CCWGG 1 cut(s) 89
MvnI CGCG 1 cut(s) 154
MwoI GCNNNNNNNGC 3 cut(s) 27, 143, 181
NciI CCSGG 1 cut(s) 15
NdeII GATC 1 cut(s) 102
NlaIV GGNNCC 3 cut(s) 80, 81, 120
NmuCI GTSAC 1 cut(s) 304
PaqCI CACCTGC 1 cut(s) 259
PctI GAATGC 2 cut(s) 259, 280
PfoI TCCNGGA 2 cut(s) 13, 87
PkrI GCNGC 5 cut(s) 139, 218, 221, 246, 280
PpuMI RGGWCCY 1 cut(s) 79
Psp5II RGGWCCY 1 cut(s) 79
Psp6I CCWGG 1 cut(s) 87
PspFI CCCAGC 1 cut(s) 114
PspGI CCWGG 1 cut(s) 87
PspN4I GGNNCC 3 cut(s) 80, 81, 120
PspPI GGNCC 1 cut(s) 79
PspPPI RGGWCCY 1 cut(s) 79
SalI GTCGAC 2 cut(s) 36, 188
SatI GCNGC 5 cut(s) 138, 217, 220, 245, 279
Sau3AI GATC 1 cut(s) 102
Sau96I GGNCC 1 cut(s) 79
ScrFI CCNGG 2 cut(s) 15, 89
SduI GDGCHC 1 cut(s) 121
SetI ASST 8 cut(s) 23, 128, 189, 239, 264, 273, 321, 327
SfaNI GCATC 1 cut(s) 165
SinI GGWCC 1 cut(s) 79
SmlI CTYRAG 1 cut(s) 229
SmoI CTYRAG 1 cut(s) 229
SsiI CCGC 5 cut(s) 112, 137, 140, 219, 278
StyD4I CCNGG 2 cut(s) 13, 87
StyI CCWWGG 1 cut(s) 258
TaaI ACNGT 3 cut(s) 35, 304, 310
TaiI ACGT 1 cut(s) 189
TaqI TCGA 4 cut(s) 37, 54, 159, 189
TauI GCSGC 3 cut(s) 140, 222, 281
TscAI CASTG 1 cut(s) 313
TseFI GTSAC 1 cut(s) 304
TseI GCWGC 2 cut(s) 216, 244
Tsp45I GTSAC 1 cut(s) 304
TspGWI ACGGA 1 cut(s) 55
TspRI CASTG 1 cut(s) 313
VpaK11BI GGWCC 1 cut(s) 79
XmiI GTMKAC 2 cut(s) 37, 189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.