Rw5G041330

kDa class I heat shock protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000537
Physical Location & Seq
Forward (+)
72834218 .. 72842113
7896 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G041330.1

Sequence Viewer

Length: 480 bp
ATGTCGCTCATTCCCAGCTTCCGAAGAAGCAGCGTCTTTGATCCCTTTTCCCTCGATCTCTGGGACCCTTTCAAGGACTTCCAATTCCATGCTTCATCGCTCTCCACTTTCCCTGAATTTCCTCGCGAAAATTCAGCTTTTGCAACAGTTAACACCAGGATTGACTGGAAGGAGACTCCAGAAGCCCATGTGTTGAAGGCCGACATTCCGGGGCTGAAGAAAGAAGAGGTCAAGGTTGAGGTTGAAGATGACGGGGTGCTTCAGATTAGCGGAGAGAGGAAGATAGAGAAGGAGGACAAGAACGACACCTGGCACCGGTTCGAGCGTAGCAGAGGCAAGTTCTCCAGAAGGTTCAGGCTTCCTGAGAATACCAAGATAGACGAGATCAAGGCTGCTATGGAGAATGGGGTTCTCAGCGTGACAATTCCTAAGGTGAAGGTGAATAAGCCCGATGTCAAAGCTATTGAAATCTCCGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

159

Amino Acids

18.32

Weight (kDa)

6.34

Isoelectric Point (pI)

45.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 55 - 158 3.1e-30 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000537)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07400 AT2G29500
fragaria_vesca FvH4_3g35270 FvH4_3g37330 FvH4_3g37360 FvH4_3g38620 FvH4_7g23650
malus_domestica MD07G1210800.v1.1 MD11G1087100.v1.1 MD11G1088300.v1.1 MD11G1089300.v1.1
prunus_persica Prupe.2G243400_v2.0.a1 Prupe.6G065900_v2.0.a1 Prupe.6G066100_v2.0.a1 Prupe.6G066200_v2.0.a1 Prupe.6G066300_v2.0.a1 Prupe.6G066400_v2.0.a1 Prupe.6G066500_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0368921 RchiOBHm_Chr1g0369001 RchiOBHm_Chr4g0444561 RchiOBHm_Chr5g0063321 RchiOBHm_Chr5g0063331 RchiOBHm_Chr5g0063361 RchiOBHm_Chr5g0067111 RchiOBHm_Chr5g0067191
rosa_laevigata RLG00000005821 RLG00000027165 RLG00000027176 RLG00000035622 RLG00000035623 RLG00000035626 RLG00000035628 RLG00000035914
rosa_multiflora Rmu_co8027810.1_g000001 Rmu_sc0000510.1_g000026 Rmu_sc0003839.1_g000004 Rmu_sc0004250.1_g000031 Rmu_sc0004250.1_g000043 Rmu_sc0005045.1_g000005 Rmu_sc0006795.1_g000002 Rmu_sc0008775.1_g000001 Rmu_sc0008775.1_g000012 Rmu_sc0012648.1_g000004
rosa_roxburghii Rroxscaffold_1G00013950 Rroxscaffold_1G00017380 Rroxscaffold_4G00288090 Rroxscaffold_4G00288200 Rroxscaffold_5G00385020
rosa_rugosa Rorug01G0345900 Rorug01G0346900 Rorug04G0353900 Rorug05G0356000 Rorug05G0356800 Rorug05G0381900 Rorug05G0382600
rosa_samantha Rh1AG353800 Rh1BG317300 Rh1CG331800 Rh1DG347900 Rh1DG348200 Rh4BG425600 Rh4CG440300 Rh4DG421200 Rh5AG415700 Rh5AG415800 Rh5AG416000 Rh5AG441900 Rh5BG430600 Rh5BG430700 Rh5BG431100 Rh5CG454100 Rh5CG454200 Rh5CG454400 Rh5DG444400 Rh5DG444500 Rh5DG444700
rosa_wichuraiana Rw1G031150 Rw1G031260 Rw4G035560 Rw5G039060 Rw5G039080 Rw5G041290 Rw5G041330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 312
AccII CGCG 1 cut(s) 126
AciI CCGC 1 cut(s) 270
AclWI GGATC 1 cut(s) 35
AcsI RAATTY 2 cut(s) 116, 130
AcuI CTGAAG 2 cut(s) 236, 245
AfiI CCNNNNNNNGG 2 cut(s) 73, 315
AgeI ACCGGT 1 cut(s) 315
AgsI TTSAA 4 cut(s) 73, 196, 245, 467
AjnI CCWGG 2 cut(s) 155, 308
AluBI AGCT 3 cut(s) 18, 137, 461
AluI AGCT 3 cut(s) 18, 137, 461
Alw26I GTCTC 1 cut(s) 167
AlwI GGATC 1 cut(s) 35
AoxI GGCC 1 cut(s) 198
ApeKI GCWGC 2 cut(s) 30, 392
ApoI RAATTY 2 cut(s) 116, 130
AsiGI ACCGGT 1 cut(s) 315
AspS9I GGNCC 1 cut(s) 64
AsuC2I CCSGG 1 cut(s) 210
AsuHPI GGTGA 2 cut(s) 445, 451
AvaII GGWCC 1 cut(s) 64
AxyI CCTNAGG 1 cut(s) 429
BanI GGYRCC 1 cut(s) 312
BbvI GCAGC 2 cut(s) 42, 379
BciT130I CCWGG 2 cut(s) 157, 310
BcnI CCSGG 1 cut(s) 210
BcoDI GTCTC 1 cut(s) 167
BisI GCNGC 2 cut(s) 31, 393
BlsI GCNGC 2 cut(s) 32, 394
Bme1390I CCNGG 3 cut(s) 157, 210, 310
Bme18I GGWCC 1 cut(s) 64
BmgT120I GGNCC 1 cut(s) 64
BmiI GGNNCC 3 cut(s) 65, 66, 314
BmrFI CCNGG 3 cut(s) 157, 210, 310
BpmI CTGGAG 2 cut(s) 162, 328
BpuMI CCSGG 1 cut(s) 210
BsaJI CCNNGG 1 cut(s) 209
BsaWI WCCGGW 2 cut(s) 315, 473
Bsc4I CCNNNNNNNGG 2 cut(s) 73, 315
Bse118I RCCGGY 1 cut(s) 315
Bse1I ACTGG 1 cut(s) 170
Bse21I CCTNAGG 1 cut(s) 429
BseBI CCWGG 2 cut(s) 157, 310
BseDI CCNNGG 1 cut(s) 209
BseLI CCNNNNNNNGG 2 cut(s) 73, 315
BseMII CTCAG 2 cut(s) 354, 427
BseNI ACTGG 1 cut(s) 170
BseXI GCAGC 2 cut(s) 42, 379
BseYI CCCAGC 1 cut(s) 14
Bsh1236I CGCG 1 cut(s) 126
BshFI GGCC 1 cut(s) 200
BshNI GGYRCC 1 cut(s) 312
BshTI ACCGGT 1 cut(s) 315
BsiSI CCGG 3 cut(s) 209, 316, 474
BslFI GGGAC 1 cut(s) 77
BslI CCNNNNNNNGG 2 cut(s) 73, 315
BsmAI GTCTC 1 cut(s) 167
BsmFI GGGAC 1 cut(s) 77
BsnI GGCC 1 cut(s) 200
Bsp143I GATC 3 cut(s) 40, 55, 384
Bsp68I TCGCGA 1 cut(s) 126
BspACI CCGC 1 cut(s) 270
BspANI GGCC 1 cut(s) 200
BspCNI CTCAG 2 cut(s) 355, 426
BspFNI CGCG 1 cut(s) 126
BspLI GGNNCC 3 cut(s) 65, 66, 314
BspPI GGATC 1 cut(s) 35
BspT107I GGYRCC 1 cut(s) 312
BsrFI RCCGGY 1 cut(s) 315
BsrI ACTGG 1 cut(s) 170
BssAI RCCGGY 1 cut(s) 315
BssECI CCNNGG 1 cut(s) 209
BssMI GATC 3 cut(s) 40, 55, 384
Bst2UI CCWGG 2 cut(s) 157, 310
Bst4CI ACNGT 1 cut(s) 148
Bst6I CTCTTC 1 cut(s) 219
BstDEI CTNAG 3 cut(s) 363, 413, 429
BstFNI CGCG 1 cut(s) 126
BstKTI GATC 3 cut(s) 43, 58, 387
BstMAI GTCTC 1 cut(s) 167
BstMBI GATC 3 cut(s) 40, 55, 384
BstNI CCWGG 2 cut(s) 157, 310
BstSCI CCNGG 3 cut(s) 155, 208, 308
BstUI CGCG 1 cut(s) 126
BstV1I GCAGC 2 cut(s) 42, 379
Bsu36I CCTNAGG 1 cut(s) 429
BsuRI GGCC 1 cut(s) 200
BtgZI GCGATG 1 cut(s) 81
BtuMI TCGCGA 1 cut(s) 126
Cfr10I RCCGGY 1 cut(s) 315
Cfr13I GGNCC 1 cut(s) 64
CseI GACGC 1 cut(s) 22
CspAI ACCGGT 1 cut(s) 315
CviAII CATG 2 cut(s) 89, 188
CviJI RGCY 9 cut(s) 18, 137, 185, 200, 214, 358, 392, 448, 461
CviKI_1 RGCY 9 cut(s) 18, 137, 185, 200, 214, 358, 392, 448, 461
DdeI CTNAG 3 cut(s) 363, 413, 429
DpnI GATC 3 cut(s) 42, 57, 386
DpnII GATC 3 cut(s) 40, 55, 384
Eam1104I CTCTTC 1 cut(s) 219
EarI CTCTTC 1 cut(s) 219
Eco47I GGWCC 1 cut(s) 64
Eco57I CTGAAG 2 cut(s) 236, 245
Eco81I CCTNAGG 1 cut(s) 429
EcoO109I RGGNCCY 1 cut(s) 64
EcoRII CCWGG 2 cut(s) 155, 308
FaeI CATG 2 cut(s) 92, 191
FaiI YATR 3 cut(s) 90, 189, 398
FaqI GGGAC 1 cut(s) 77
FatI CATG 2 cut(s) 88, 187
Fnu4HI GCNGC 2 cut(s) 31, 393
Fsp4HI GCNGC 2 cut(s) 31, 393
GluI GCNGC 2 cut(s) 31, 393
GsaI CCCAGC 1 cut(s) 18
GsuI CTGGAG 2 cut(s) 162, 328
HaeIII GGCC 1 cut(s) 200
HapII CCGG 3 cut(s) 209, 316, 474
HgaI GACGC 1 cut(s) 22
Hin1II CATG 2 cut(s) 92, 191
HincII GTYRAC 1 cut(s) 151
HindII GTYRAC 1 cut(s) 151
HinfI GANTC 1 cut(s) 175
HpaI GTTAAC 1 cut(s) 151
HpaII CCGG 3 cut(s) 209, 316, 474
HphI GGTGA 2 cut(s) 445, 451
Hpy166II GTNNAC 1 cut(s) 151
Hpy188I TCNGA 2 cut(s) 23, 264
Hpy188III TCNNGA 4 cut(s) 125, 179, 345, 362
Hpy8I GTNNAC 1 cut(s) 151
HpyAV CCTTC 5 cut(s) 163, 190, 283, 342, 430
HpyCH4III ACNGT 1 cut(s) 148
HpyCH4V TGCA 1 cut(s) 143
HpyF3I CTNAG 3 cut(s) 363, 413, 429
Hsp92II CATG 2 cut(s) 92, 191
KflI GGGWCCC 1 cut(s) 64
KspAI GTTAAC 1 cut(s) 151
Kzo9I GATC 3 cut(s) 40, 55, 384
Lsp1109I GCAGC 2 cut(s) 42, 379
MaeIII GTNAC 1 cut(s) 418
MalI GATC 3 cut(s) 42, 57, 386
MboI GATC 3 cut(s) 40, 55, 384
MboII GAAGA 5 cut(s) 36, 229, 236, 257, 292
MluCI AATT 4 cut(s) 83, 116, 130, 423
MlyI GAGTC 1 cut(s) 169
MnlI CCTC 7 cut(s) 62, 132, 220, 232, 270, 286, 326
MseI TTAA 1 cut(s) 150
MspI CCGG 3 cut(s) 209, 316, 474
MspR9I CCNGG 3 cut(s) 157, 210, 310
MvaI CCWGG 2 cut(s) 157, 310
MvnI CGCG 1 cut(s) 126
NciI CCSGG 1 cut(s) 210
NdeII GATC 3 cut(s) 40, 55, 384
NlaIII CATG 2 cut(s) 92, 191
NlaIV GGNNCC 3 cut(s) 65, 66, 314
NmuCI GTSAC 1 cut(s) 418
NruI TCGCGA 1 cut(s) 126
PinAI ACCGGT 1 cut(s) 315
PkrI GCNGC 2 cut(s) 32, 394
PleI GAGTC 1 cut(s) 169
PpsI GAGTC 1 cut(s) 169
PpuMI RGGWCCY 1 cut(s) 64
Psp5II RGGWCCY 1 cut(s) 64
Psp6I CCWGG 2 cut(s) 155, 308
PspFI CCCAGC 1 cut(s) 14
PspGI CCWGG 2 cut(s) 155, 308
PspN4I GGNNCC 3 cut(s) 65, 66, 314
PspPI GGNCC 1 cut(s) 64
PspPPI RGGWCCY 1 cut(s) 64
RruI TCGCGA 1 cut(s) 126
SaqAI TTAA 1 cut(s) 150
SatI GCNGC 2 cut(s) 31, 393
Sau3AI GATC 3 cut(s) 40, 55, 384
Sau96I GGNCC 1 cut(s) 64
SchI GAGTC 1 cut(s) 169
ScrFI CCNGG 3 cut(s) 157, 210, 310
SinI GGWCC 1 cut(s) 64
Sse9I AATT 4 cut(s) 83, 116, 130, 423
SsiI CCGC 1 cut(s) 270
StyD4I CCNGG 3 cut(s) 155, 208, 308
TaaI ACNGT 1 cut(s) 148
TaqI TCGA 2 cut(s) 54, 321
TasI AATT 4 cut(s) 83, 116, 130, 423
Tru1I TTAA 1 cut(s) 150
Tru9I TTAA 1 cut(s) 150
TseFI GTSAC 1 cut(s) 418
TseI GCWGC 2 cut(s) 30, 392
Tsp45I GTSAC 1 cut(s) 418
TspDTI ATGAA 1 cut(s) 84
VpaK11BI GGWCC 1 cut(s) 64
XapI RAATTY 2 cut(s) 116, 130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.