RLG00000005821

Hsp20/alpha crystallin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0000537
Physical Location & Seq
Reverse (-)
2158309 .. 2158785
477 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005821

Sequence Viewer

Length: 477 bp
ATGTCTCTCATTCCGGGAAGCTTTTTCGGCAACGGTCGACGGAGTAATATCTTCGACCCGTTCTCGCTCGACATCTGGGACCCGTTCCAAGACTCCCCCTTGATCAACTCCCGCTCGACTCCAAGCTCAGAGACCGCGGCGGTGGCGAACACGCGCATAGACTGGAAGGAGACGCCGGAGGCGCACGTGTTCAAGGCGGACCTGCCGGGGCTGAAGAAGGAGGAGGTGAAGGTGGAGGTGGAGGAAGGGAAGGTGCTGCAGATCAGCGGCGAGAGGAGAGTGGAGAAGGAGGATAAGAGCGACAAGTGGCACAGGATGGAGAGGAGCAGCGGCAAGTTCTTGAGGAGGTTCAGGCTGCCGGAGAATGCCAAGGTGGAGCAGGTGAAGGCGGAAATGGAGAATGGGGTGGTTACTGTCACTGTGCCAAAGGTGGAGGTCAAGAAGCCTGATGTTAAGGCCATTCAGATTTCGGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

159

Amino Acids

17.95

Weight (kDa)

6.77

Isoelectric Point (pI)

52.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 54 - 157 1e-30 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000537)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07400 AT2G29500
fragaria_vesca FvH4_3g35270 FvH4_3g37330 FvH4_3g37360 FvH4_3g38620 FvH4_7g23650
malus_domestica MD07G1210800.v1.1 MD11G1087100.v1.1 MD11G1088300.v1.1 MD11G1089300.v1.1
prunus_persica Prupe.2G243400_v2.0.a1 Prupe.6G065900_v2.0.a1 Prupe.6G066100_v2.0.a1 Prupe.6G066200_v2.0.a1 Prupe.6G066300_v2.0.a1 Prupe.6G066400_v2.0.a1 Prupe.6G066500_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0368921 RchiOBHm_Chr1g0369001 RchiOBHm_Chr4g0444561 RchiOBHm_Chr5g0063321 RchiOBHm_Chr5g0063331 RchiOBHm_Chr5g0063361 RchiOBHm_Chr5g0067111 RchiOBHm_Chr5g0067191
rosa_laevigata RLG00000005821 RLG00000027165 RLG00000027176 RLG00000035622 RLG00000035623 RLG00000035626 RLG00000035628 RLG00000035914
rosa_multiflora Rmu_co8027810.1_g000001 Rmu_sc0000510.1_g000026 Rmu_sc0003839.1_g000004 Rmu_sc0004250.1_g000031 Rmu_sc0004250.1_g000043 Rmu_sc0005045.1_g000005 Rmu_sc0006795.1_g000002 Rmu_sc0008775.1_g000001 Rmu_sc0008775.1_g000012 Rmu_sc0012648.1_g000004
rosa_roxburghii Rroxscaffold_1G00013950 Rroxscaffold_1G00017380 Rroxscaffold_4G00288090 Rroxscaffold_4G00288200 Rroxscaffold_5G00385020
rosa_rugosa Rorug01G0345900 Rorug01G0346900 Rorug04G0353900 Rorug05G0356000 Rorug05G0356800 Rorug05G0381900 Rorug05G0382600
rosa_samantha Rh1AG353800 Rh1BG317300 Rh1CG331800 Rh1DG347900 Rh1DG348200 Rh4BG425600 Rh4CG440300 Rh4DG421200 Rh5AG415700 Rh5AG415800 Rh5AG416000 Rh5AG441900 Rh5BG430600 Rh5BG430700 Rh5BG431100 Rh5CG454100 Rh5CG454200 Rh5CG454400 Rh5DG444400 Rh5DG444500 Rh5DG444700
rosa_wichuraiana Rw1G031150 Rw1G031260 Rw4G035560 Rw5G039060 Rw5G039080 Rw5G041290 Rw5G041330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 370
Acc36I ACCTGC 2 cut(s) 210, 370
AccBSI CCGCTC 1 cut(s) 114
AccI GTMKAC 1 cut(s) 37
AccII CGCG 2 cut(s) 137, 154
AciI CCGC 8 cut(s) 112, 135, 137, 140, 197, 267, 330, 389
AcuI CTGAAG 1 cut(s) 233
AcvI CACGTG 1 cut(s) 187
AcyI GRCGYC 1 cut(s) 173
AflIII ACRYGT 1 cut(s) 186
AgsI TTSAA 1 cut(s) 193
AluBI AGCT 2 cut(s) 21, 126
AluI AGCT 2 cut(s) 21, 126
Alw26I GTCTC 3 cut(s) 9, 125, 164
AoxI GGCC 1 cut(s) 456
ApeKI GCWGC 3 cut(s) 256, 327, 355
AspLEI GCGC 2 cut(s) 156, 184
AspS9I GGNCC 2 cut(s) 79, 199
AsuC2I CCSGG 2 cut(s) 15, 207
AsuHPI GGTGA 2 cut(s) 238, 394
AvaII GGWCC 2 cut(s) 79, 199
BbrPI CACGTG 1 cut(s) 187
BbvI GCAGC 3 cut(s) 243, 339, 342
BccI CCATC 1 cut(s) 310
BclI TGATCA 1 cut(s) 102
BcnI CCSGG 2 cut(s) 15, 207
BcoDI GTCTC 3 cut(s) 9, 125, 164
BfmI CTRYAG 1 cut(s) 257
BfuAI ACCTGC 2 cut(s) 210, 370
BisI GCNGC 6 cut(s) 138, 257, 268, 328, 331, 356
BlsI GCNGC 6 cut(s) 139, 258, 269, 329, 332, 357
Bme1390I CCNGG 2 cut(s) 15, 207
Bme18I GGWCC 2 cut(s) 79, 199
BmgT120I GGNCC 2 cut(s) 79, 199
BmiI GGNNCC 2 cut(s) 80, 81
BmrFI CCNGG 2 cut(s) 15, 207
BpuEI CTTGAG 1 cut(s) 361
BpuMI CCSGG 2 cut(s) 15, 207
BsaAI YACGTR 1 cut(s) 187
BsaHI GRCGYC 1 cut(s) 173
BsaI GGTCTC 1 cut(s) 125
BsaJI CCNNGG 3 cut(s) 135, 206, 369
Bse1I ACTGG 1 cut(s) 167
BseDI CCNNGG 3 cut(s) 135, 206, 369
BseGI GGATG 1 cut(s) 321
BseMII CTCAG 1 cut(s) 141
BseNI ACTGG 1 cut(s) 167
BseRI GAGGAG 4 cut(s) 236, 289, 337, 358
BseXI GCAGC 3 cut(s) 243, 339, 342
Bsh1236I CGCG 2 cut(s) 137, 154
Bsh1285I CGRYCG 1 cut(s) 37
BshFI GGCC 1 cut(s) 458
BsiEI CGRYCG 1 cut(s) 37
BsiSI CCGG 4 cut(s) 14, 176, 206, 359
BslFI GGGAC 1 cut(s) 92
BsmAI GTCTC 3 cut(s) 9, 125, 164
BsmBI CGTCTC 1 cut(s) 164
BsmFI GGGAC 1 cut(s) 92
BsmI GAATGC 1 cut(s) 370
BsnI GGCC 1 cut(s) 458
Bso31I GGTCTC 1 cut(s) 125
Bsp143I GATC 2 cut(s) 102, 261
BspACI CCGC 8 cut(s) 112, 135, 137, 140, 197, 267, 330, 389
BspANI GGCC 1 cut(s) 458
BspCNI CTCAG 1 cut(s) 140
BspFNI CGCG 2 cut(s) 137, 154
BspLI GGNNCC 2 cut(s) 80, 81
BspMAI CTGCAG 1 cut(s) 261
BspMI ACCTGC 2 cut(s) 210, 370
BspTNI GGTCTC 1 cut(s) 125
BsrBI CCGCTC 1 cut(s) 114
BsrI ACTGG 1 cut(s) 167
BssECI CCNNGG 3 cut(s) 135, 206, 369
BssMI GATC 2 cut(s) 102, 261
BssNI GRCGYC 1 cut(s) 173
BssT1I CCWWGG 1 cut(s) 369
Bst4CI ACNGT 3 cut(s) 35, 415, 421
BstACI GRCGYC 1 cut(s) 173
BstBAI YACGTR 1 cut(s) 187
BstDEI CTNAG 1 cut(s) 127
BstDSI CCRYGG 1 cut(s) 135
BstF5I GGATG 1 cut(s) 321
BstFNI CGCG 2 cut(s) 137, 154
BstHHI GCGC 2 cut(s) 156, 184
BstKTI GATC 2 cut(s) 105, 264
BstMAI GTCTC 3 cut(s) 9, 125, 164
BstMBI GATC 2 cut(s) 102, 261
BstMCI CGRYCG 1 cut(s) 37
BstMWI GCNNNNNNNGC 3 cut(s) 27, 143, 181
BstSCI CCNGG 2 cut(s) 13, 205
BstSFI CTRYAG 1 cut(s) 257
BstUI CGCG 2 cut(s) 137, 154
BstV1I GCAGC 3 cut(s) 243, 339, 342
BsuRI GGCC 1 cut(s) 458
BtgI CCRYGG 1 cut(s) 135
BtsCI GGATG 1 cut(s) 321
BtsIMutI CAGTG 1 cut(s) 417
BveI ACCTGC 2 cut(s) 210, 370
CfoI GCGC 2 cut(s) 156, 184
Cfr13I GGNCC 2 cut(s) 79, 199
Cfr42I CCGCGG 1 cut(s) 138
CseI GACGC 1 cut(s) 181
CviJI RGCY 6 cut(s) 21, 126, 211, 355, 445, 458
CviKI_1 RGCY 6 cut(s) 21, 126, 211, 355, 445, 458
DdeI CTNAG 1 cut(s) 127
DpnI GATC 2 cut(s) 104, 263
DpnII GATC 2 cut(s) 102, 261
EciI GGCGGA 2 cut(s) 212, 404
Eco130I CCWWGG 1 cut(s) 369
Eco31I GGTCTC 1 cut(s) 125
Eco47I GGWCC 2 cut(s) 79, 199
Eco57I CTGAAG 1 cut(s) 233
Eco72I CACGTG 1 cut(s) 187
EcoO109I RGGNCCY 1 cut(s) 79
EcoT14I CCWWGG 1 cut(s) 369
ErhI CCWWGG 1 cut(s) 369
Esp3I CGTCTC 1 cut(s) 164
FaiI YATR 1 cut(s) 158
FaqI GGGAC 1 cut(s) 92
FauI CCCGC 1 cut(s) 119
FbaI TGATCA 1 cut(s) 102
FblI GTMKAC 1 cut(s) 37
Fnu4HI GCNGC 6 cut(s) 138, 257, 268, 328, 331, 356
FokI GGATG 1 cut(s) 328
Fsp4HI GCNGC 6 cut(s) 138, 257, 268, 328, 331, 356
GlaI GCGC 2 cut(s) 155, 183
GluI GCNGC 6 cut(s) 138, 257, 268, 328, 331, 356
HaeIII GGCC 1 cut(s) 458
HapII CCGG 4 cut(s) 14, 176, 206, 359
HgaI GACGC 1 cut(s) 181
HhaI GCGC 2 cut(s) 156, 184
Hin1I GRCGYC 1 cut(s) 173
Hin6I GCGC 2 cut(s) 154, 182
HinP1I GCGC 2 cut(s) 154, 182
HincII GTYRAC 1 cut(s) 38
HindII GTYRAC 1 cut(s) 38
HindIII AAGCTT 1 cut(s) 19
HinfI GANTC 2 cut(s) 92, 118
HpaII CCGG 4 cut(s) 14, 176, 206, 359
HphI GGTGA 2 cut(s) 238, 394
Hpy166II GTNNAC 1 cut(s) 38
Hpy188I TCNGA 3 cut(s) 130, 465, 472
Hpy188III TCNNGA 2 cut(s) 340, 439
Hpy8I GTNNAC 1 cut(s) 38
Hpy99I CGWCG 1 cut(s) 42
HpyAV CCTTC 7 cut(s) 160, 211, 223, 239, 244, 280, 379
HpyCH4III ACNGT 3 cut(s) 35, 415, 421
HpyCH4IV ACGT 1 cut(s) 186
HpyCH4V TGCA 1 cut(s) 259
HpyF10VI GCNNNNNNNGC 3 cut(s) 27, 143, 181
HpyF3I CTNAG 1 cut(s) 127
HpySE526I ACGT 1 cut(s) 186
Hsp92I GRCGYC 1 cut(s) 173
HspAI GCGC 2 cut(s) 154, 182
KflI GGGWCCC 1 cut(s) 79
Ksp22I TGATCA 1 cut(s) 102
KspI CCGCGG 1 cut(s) 138
Kzo9I GATC 2 cut(s) 102, 261
LmnI GCTCC 2 cut(s) 324, 376
Lsp1109I GCAGC 3 cut(s) 243, 339, 342
MaeII ACGT 1 cut(s) 186
MaeIII GTNAC 2 cut(s) 409, 415
MalI GATC 2 cut(s) 104, 263
MbiI CCGCTC 1 cut(s) 114
MboI GATC 2 cut(s) 102, 261
MboII GAAGA 2 cut(s) 43, 226
MlyI GAGTC 2 cut(s) 86, 112
MseI TTAA 1 cut(s) 453
MspA1I CMGCKG 3 cut(s) 137, 267, 330
MspI CCGG 4 cut(s) 14, 176, 206, 359
MspR9I CCNGG 2 cut(s) 15, 207
Mva1269I GAATGC 1 cut(s) 370
MvnI CGCG 2 cut(s) 137, 154
MwoI GCNNNNNNNGC 3 cut(s) 27, 143, 181
NciI CCSGG 2 cut(s) 15, 207
NdeII GATC 2 cut(s) 102, 261
NlaIV GGNNCC 2 cut(s) 80, 81
NmuCI GTSAC 1 cut(s) 415
PaqCI CACCTGC 1 cut(s) 370
PctI GAATGC 1 cut(s) 370
PfoI TCCNGGA 1 cut(s) 13
PkrI GCNGC 6 cut(s) 139, 258, 269, 329, 332, 357
PleI GAGTC 2 cut(s) 86, 112
PmaCI CACGTG 1 cut(s) 187
PmlI CACGTG 1 cut(s) 187
PpsI GAGTC 2 cut(s) 86, 112
Ppu21I YACGTR 1 cut(s) 187
PpuMI RGGWCCY 1 cut(s) 79
Psp5II RGGWCCY 1 cut(s) 79
PspCI CACGTG 1 cut(s) 187
PspN4I GGNNCC 2 cut(s) 80, 81
PspPI GGNCC 2 cut(s) 79, 199
PspPPI RGGWCCY 1 cut(s) 79
PstI CTGCAG 1 cut(s) 261
SacII CCGCGG 1 cut(s) 138
SalI GTCGAC 1 cut(s) 36
SaqAI TTAA 1 cut(s) 453
SatI GCNGC 6 cut(s) 138, 257, 268, 328, 331, 356
Sau3AI GATC 2 cut(s) 102, 261
Sau96I GGNCC 2 cut(s) 79, 199
SchI GAGTC 2 cut(s) 86, 112
ScrFI CCNGG 2 cut(s) 15, 207
SfcI CTRYAG 1 cut(s) 257
Sfr303I CCGCGG 1 cut(s) 138
SgrBI CCGCGG 1 cut(s) 138
SinI GGWCC 2 cut(s) 79, 199
SmlI CTYRAG 1 cut(s) 340
SmoI CTYRAG 1 cut(s) 340
SsiI CCGC 8 cut(s) 112, 135, 137, 140, 197, 267, 330, 389
StyD4I CCNGG 2 cut(s) 13, 205
StyI CCWWGG 1 cut(s) 369
TaaI ACNGT 3 cut(s) 35, 415, 421
TaiI ACGT 1 cut(s) 189
TaqI TCGA 4 cut(s) 37, 54, 69, 116
TauI GCSGC 3 cut(s) 140, 270, 333
Tru1I TTAA 1 cut(s) 453
Tru9I TTAA 1 cut(s) 453
TscAI CASTG 1 cut(s) 424
TseFI GTSAC 1 cut(s) 415
TseI GCWGC 3 cut(s) 256, 327, 355
Tsp45I GTSAC 1 cut(s) 415
TspGWI ACGGA 1 cut(s) 55
TspRI CASTG 1 cut(s) 424
VpaK11BI GGWCC 2 cut(s) 79, 199
XmiI GTMKAC 1 cut(s) 37
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.