Rroxscaffold_5G00385020

Hsp20/alpha crystallin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000537
Physical Location & Seq
Forward (+)
64197878 .. 64198565
688 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00385020.1

Sequence Viewer

Length: 546 bp
ATGCTTTCTATTGACTTTTCGAGAACCATCTCTCTGGAACCTCCTTTGGCCAGACAATACCAGACACTTCAGGAAGAGACGAAGAATCCCTTTTTCGGCAACGGTCGACGGAGTAACATCTTCGACCCGTTCTCGCTGGACATCTGGGACCCGTTTCAGGACTTCCCCTTGATCAACTCCCGCTCGGCTCCAAGCTCAGAGACGGCGGCAGTGGCGAACACGCGCATCGACTGGAAGGAGACGCCGGAGGCGCACGTGTTCAAGGCGGACCTGCCGGGGCTGAAGAAGGAGGAGGTGAAGGTGGAGGTGGAGGAAGGGAAGGTGCTGCAGATCAGCGGCGAGAGGAGAGTGGAGAAGGAGGACAAAAGCGACAAGTGGCACAGGATGGAGAGGAGCAGAGGCAAGTTCGTGAGGAGGTTCAGGCTGCCGGAGAATGCCAGGGTGGAGCAGGTGAAGGCGGCAATGGAGAATGGGGTGCTTACTGTCACTGTGCCAAAGGTGGAGGTCAAGAAGCCTGATGTGAAGGCCATTCAGATTTCTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

181

Amino Acids

20.82

Weight (kDa)

7.91

Isoelectric Point (pI)

51.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 77 - 180 1e-31 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000537)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07400 AT2G29500
fragaria_vesca FvH4_3g35270 FvH4_3g37330 FvH4_3g37360 FvH4_3g38620 FvH4_7g23650
malus_domestica MD07G1210800.v1.1 MD11G1087100.v1.1 MD11G1088300.v1.1 MD11G1089300.v1.1
prunus_persica Prupe.2G243400_v2.0.a1 Prupe.6G065900_v2.0.a1 Prupe.6G066100_v2.0.a1 Prupe.6G066200_v2.0.a1 Prupe.6G066300_v2.0.a1 Prupe.6G066400_v2.0.a1 Prupe.6G066500_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0368921 RchiOBHm_Chr1g0369001 RchiOBHm_Chr4g0444561 RchiOBHm_Chr5g0063321 RchiOBHm_Chr5g0063331 RchiOBHm_Chr5g0063361 RchiOBHm_Chr5g0067111 RchiOBHm_Chr5g0067191
rosa_laevigata RLG00000005821 RLG00000027165 RLG00000027176 RLG00000035622 RLG00000035623 RLG00000035626 RLG00000035628 RLG00000035914
rosa_multiflora Rmu_co8027810.1_g000001 Rmu_sc0000510.1_g000026 Rmu_sc0003839.1_g000004 Rmu_sc0004250.1_g000031 Rmu_sc0004250.1_g000043 Rmu_sc0005045.1_g000005 Rmu_sc0006795.1_g000002 Rmu_sc0008775.1_g000001 Rmu_sc0008775.1_g000012 Rmu_sc0012648.1_g000004
rosa_roxburghii Rroxscaffold_1G00013950 Rroxscaffold_1G00017380 Rroxscaffold_4G00288090 Rroxscaffold_4G00288200 Rroxscaffold_5G00385020
rosa_rugosa Rorug01G0345900 Rorug01G0346900 Rorug04G0353900 Rorug05G0356000 Rorug05G0356800 Rorug05G0381900 Rorug05G0382600
rosa_samantha Rh1AG353800 Rh1BG317300 Rh1CG331800 Rh1DG347900 Rh1DG348200 Rh4BG425600 Rh4CG440300 Rh4DG421200 Rh5AG415700 Rh5AG415800 Rh5AG416000 Rh5AG441900 Rh5BG430600 Rh5BG430700 Rh5BG431100 Rh5CG454100 Rh5CG454200 Rh5CG454400 Rh5DG444400 Rh5DG444500 Rh5DG444700
rosa_wichuraiana Rw1G031150 Rw1G031260 Rw4G035560 Rw5G039060 Rw5G039080 Rw5G041290 Rw5G041330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 439
Acc36I ACCTGC 2 cut(s) 279, 439
AccBSI CCGCTC 1 cut(s) 183
AccI GTMKAC 1 cut(s) 106
AccII CGCG 1 cut(s) 223
AciI CCGC 5 cut(s) 181, 206, 266, 336, 458
AcoI YGGCCR 1 cut(s) 48
AcuI CTGAAG 2 cut(s) 53, 302
AcvI CACGTG 1 cut(s) 256
AcyI GRCGYC 1 cut(s) 242
AfiI CCNNNNNNNGG 2 cut(s) 95, 157
AflIII ACRYGT 1 cut(s) 255
AgsI TTSAA 1 cut(s) 262
AjnI CCWGG 1 cut(s) 437
AluBI AGCT 1 cut(s) 195
AluI AGCT 1 cut(s) 195
Alw26I GTCTC 3 cut(s) 71, 194, 233
AoxI GGCC 2 cut(s) 48, 525
ApeKI GCWGC 2 cut(s) 325, 424
AspLEI GCGC 2 cut(s) 225, 253
AspS9I GGNCC 2 cut(s) 148, 268
AsuC2I CCSGG 1 cut(s) 276
AsuHPI GGTGA 2 cut(s) 307, 463
AvaII GGWCC 2 cut(s) 148, 268
BalI TGGCCA 1 cut(s) 50
BbrPI CACGTG 1 cut(s) 256
BbvI GCAGC 2 cut(s) 312, 411
BccI CCATC 2 cut(s) 35, 379
BceAI ACGGC 1 cut(s) 219
BciT130I CCWGG 1 cut(s) 439
BclI TGATCA 1 cut(s) 171
BcnI CCSGG 1 cut(s) 276
BcoDI GTCTC 3 cut(s) 71, 194, 233
BfmI CTRYAG 1 cut(s) 326
BfuAI ACCTGC 2 cut(s) 279, 439
BisI GCNGC 5 cut(s) 207, 326, 337, 425, 459
BlsI GCNGC 5 cut(s) 208, 327, 338, 426, 460
Bme1390I CCNGG 2 cut(s) 276, 439
Bme18I GGWCC 2 cut(s) 148, 268
BmgT120I GGNCC 2 cut(s) 148, 268
BmiI GGNNCC 4 cut(s) 39, 149, 150, 189
BmrFI CCNGG 2 cut(s) 276, 439
BmsI GCATC 1 cut(s) 234
BpuMI CCSGG 1 cut(s) 276
BsaAI YACGTR 1 cut(s) 256
BsaHI GRCGYC 1 cut(s) 242
BsaJI CCNNGG 2 cut(s) 275, 438
Bsc4I CCNNNNNNNGG 2 cut(s) 95, 157
Bse1I ACTGG 1 cut(s) 236
Bse3DI GCAATG 1 cut(s) 468
BseBI CCWGG 1 cut(s) 439
BseDI CCNNGG 2 cut(s) 275, 438
BseGI GGATG 1 cut(s) 390
BseLI CCNNNNNNNGG 2 cut(s) 95, 157
BseMI GCAATG 1 cut(s) 468
BseMII CTCAG 1 cut(s) 210
BseNI ACTGG 1 cut(s) 236
BseRI GAGGAG 4 cut(s) 305, 358, 406, 427
BseXI GCAGC 2 cut(s) 312, 411
Bsh1236I CGCG 1 cut(s) 223
Bsh1285I CGRYCG 1 cut(s) 106
BshFI GGCC 2 cut(s) 50, 527
BsiEI CGRYCG 1 cut(s) 106
BsiSI CCGG 3 cut(s) 245, 275, 428
BslFI GGGAC 1 cut(s) 161
BslI CCNNNNNNNGG 2 cut(s) 95, 157
BsmAI GTCTC 3 cut(s) 71, 194, 233
BsmBI CGTCTC 3 cut(s) 71, 194, 233
BsmFI GGGAC 1 cut(s) 161
BsmI GAATGC 1 cut(s) 439
BsnI GGCC 2 cut(s) 50, 527
Bsp143I GATC 2 cut(s) 171, 330
BspACI CCGC 5 cut(s) 181, 206, 266, 336, 458
BspANI GGCC 2 cut(s) 50, 527
BspCNI CTCAG 1 cut(s) 209
BspFNI CGCG 1 cut(s) 223
BspLI GGNNCC 4 cut(s) 39, 149, 150, 189
BspMAI CTGCAG 1 cut(s) 330
BspMI ACCTGC 2 cut(s) 279, 439
BsrBI CCGCTC 1 cut(s) 183
BsrDI GCAATG 1 cut(s) 468
BsrI ACTGG 1 cut(s) 236
BssECI CCNNGG 2 cut(s) 275, 438
BssMI GATC 2 cut(s) 171, 330
BssNI GRCGYC 1 cut(s) 242
Bst2UI CCWGG 1 cut(s) 439
Bst4CI ACNGT 3 cut(s) 104, 484, 490
Bst6I CTCTTC 1 cut(s) 69
BstACI GRCGYC 1 cut(s) 242
BstBAI YACGTR 1 cut(s) 256
BstDEI CTNAG 1 cut(s) 196
BstF5I GGATG 1 cut(s) 390
BstFNI CGCG 1 cut(s) 223
BstHHI GCGC 2 cut(s) 225, 253
BstKTI GATC 2 cut(s) 174, 333
BstMAI GTCTC 3 cut(s) 71, 194, 233
BstMBI GATC 2 cut(s) 171, 330
BstMCI CGRYCG 1 cut(s) 106
BstMWI GCNNNNNNNGC 2 cut(s) 212, 250
BstNI CCWGG 1 cut(s) 439
BstSCI CCNGG 2 cut(s) 274, 437
BstSFI CTRYAG 1 cut(s) 326
BstUI CGCG 1 cut(s) 223
BstV1I GCAGC 2 cut(s) 312, 411
BstXI CCANNNNNNTGG 1 cut(s) 34
BsuRI GGCC 2 cut(s) 50, 527
BtsCI GGATG 1 cut(s) 390
BtsI GCAGTG 1 cut(s) 216
BtsIMutI CAGTG 2 cut(s) 216, 486
BveI ACCTGC 2 cut(s) 279, 439
CfoI GCGC 2 cut(s) 225, 253
Cfr13I GGNCC 2 cut(s) 148, 268
CseI GACGC 1 cut(s) 250
CviJI RGCY 7 cut(s) 50, 188, 195, 280, 424, 514, 527
CviKI_1 RGCY 7 cut(s) 50, 188, 195, 280, 424, 514, 527
DdeI CTNAG 1 cut(s) 196
DpnI GATC 2 cut(s) 173, 332
DpnII GATC 2 cut(s) 171, 330
EaeI YGGCCR 1 cut(s) 48
Eam1104I CTCTTC 1 cut(s) 69
EarI CTCTTC 1 cut(s) 69
EciI GGCGGA 1 cut(s) 281
Eco47I GGWCC 2 cut(s) 148, 268
Eco57I CTGAAG 2 cut(s) 53, 302
Eco72I CACGTG 1 cut(s) 256
EcoO109I RGGNCCY 1 cut(s) 148
EcoRII CCWGG 1 cut(s) 437
Esp3I CGTCTC 3 cut(s) 71, 194, 233
FalI AAGNNNNNCTT 2 cut(s) 74, 106
FaqI GGGAC 1 cut(s) 161
FauI CCCGC 1 cut(s) 188
FbaI TGATCA 1 cut(s) 171
FblI GTMKAC 1 cut(s) 106
Fnu4HI GCNGC 5 cut(s) 207, 326, 337, 425, 459
FokI GGATG 1 cut(s) 397
Fsp4HI GCNGC 5 cut(s) 207, 326, 337, 425, 459
GlaI GCGC 2 cut(s) 224, 252
GluI GCNGC 5 cut(s) 207, 326, 337, 425, 459
HaeIII GGCC 2 cut(s) 50, 527
HapII CCGG 3 cut(s) 245, 275, 428
HgaI GACGC 1 cut(s) 250
HhaI GCGC 2 cut(s) 225, 253
Hin1I GRCGYC 1 cut(s) 242
Hin6I GCGC 2 cut(s) 223, 251
HinP1I GCGC 2 cut(s) 223, 251
HincII GTYRAC 1 cut(s) 107
HindII GTYRAC 1 cut(s) 107
HinfI GANTC 1 cut(s) 85
HpaII CCGG 3 cut(s) 245, 275, 428
HphI GGTGA 2 cut(s) 307, 463
Hpy166II GTNNAC 1 cut(s) 107
Hpy188I TCNGA 3 cut(s) 199, 534, 541
Hpy188III TCNNGA 6 cut(s) 21, 35, 71, 158, 409, 508
Hpy8I GTNNAC 1 cut(s) 107
Hpy99I CGWCG 1 cut(s) 111
HpyAV CCTTC 8 cut(s) 229, 280, 292, 308, 313, 349, 448, 517
HpyCH4III ACNGT 3 cut(s) 104, 484, 490
HpyCH4IV ACGT 1 cut(s) 255
HpyCH4V TGCA 1 cut(s) 328
HpyF10VI GCNNNNNNNGC 2 cut(s) 212, 250
HpyF3I CTNAG 1 cut(s) 196
HpySE526I ACGT 1 cut(s) 255
Hsp92I GRCGYC 1 cut(s) 242
HspAI GCGC 2 cut(s) 223, 251
KflI GGGWCCC 1 cut(s) 148
Ksp22I TGATCA 1 cut(s) 171
Kzo9I GATC 2 cut(s) 171, 330
LmnI GCTCC 3 cut(s) 193, 393, 445
Lsp1109I GCAGC 2 cut(s) 312, 411
LweI GCATC 1 cut(s) 234
MaeII ACGT 1 cut(s) 255
MaeIII GTNAC 2 cut(s) 113, 484
MalI GATC 2 cut(s) 173, 332
MbiI CCGCTC 1 cut(s) 183
MboI GATC 2 cut(s) 171, 330
MboII GAAGA 4 cut(s) 86, 94, 112, 295
MlsI TGGCCA 1 cut(s) 50
MluNI TGGCCA 1 cut(s) 50
Mox20I TGGCCA 1 cut(s) 50
MscI TGGCCA 1 cut(s) 50
Msp20I TGGCCA 1 cut(s) 50
MspA1I CMGCKG 1 cut(s) 336
MspI CCGG 3 cut(s) 245, 275, 428
MspR9I CCNGG 2 cut(s) 276, 439
Mva1269I GAATGC 1 cut(s) 439
MvaI CCWGG 1 cut(s) 439
MvnI CGCG 1 cut(s) 223
MwoI GCNNNNNNNGC 2 cut(s) 212, 250
NciI CCSGG 1 cut(s) 276
NdeII GATC 2 cut(s) 171, 330
NlaIV GGNNCC 4 cut(s) 39, 149, 150, 189
NmeAIII GCCGAG 1 cut(s) 164
NmuCI GTSAC 1 cut(s) 484
PaqCI CACCTGC 1 cut(s) 439
PctI GAATGC 1 cut(s) 439
PfeI GAWTC 1 cut(s) 85
PkrI GCNGC 5 cut(s) 208, 327, 338, 426, 460
PmaCI CACGTG 1 cut(s) 256
PmlI CACGTG 1 cut(s) 256
Ppu21I YACGTR 1 cut(s) 256
PpuMI RGGWCCY 1 cut(s) 148
Psp5II RGGWCCY 1 cut(s) 148
Psp6I CCWGG 1 cut(s) 437
PspCI CACGTG 1 cut(s) 256
PspGI CCWGG 1 cut(s) 437
PspN4I GGNNCC 4 cut(s) 39, 149, 150, 189
PspPI GGNCC 2 cut(s) 148, 268
PspPPI RGGWCCY 1 cut(s) 148
PstI CTGCAG 1 cut(s) 330
SalI GTCGAC 1 cut(s) 105
SatI GCNGC 5 cut(s) 207, 326, 337, 425, 459
Sau3AI GATC 2 cut(s) 171, 330
Sau96I GGNCC 2 cut(s) 148, 268
ScrFI CCNGG 2 cut(s) 276, 439
SfaNI GCATC 1 cut(s) 234
SfcI CTRYAG 1 cut(s) 326
SinI GGWCC 2 cut(s) 148, 268
SsiI CCGC 5 cut(s) 181, 206, 266, 336, 458
StyD4I CCNGG 2 cut(s) 274, 437
TaaI ACNGT 3 cut(s) 104, 484, 490
TaiI ACGT 1 cut(s) 258
TaqI TCGA 4 cut(s) 20, 106, 123, 228
TauI GCSGC 3 cut(s) 209, 339, 461
TfiI GAWTC 1 cut(s) 85
TscAI CASTG 2 cut(s) 216, 493
TseFI GTSAC 1 cut(s) 484
TseI GCWGC 2 cut(s) 325, 424
Tsp45I GTSAC 1 cut(s) 484
TspGWI ACGGA 1 cut(s) 124
TspRI CASTG 2 cut(s) 216, 493
VpaK11BI GGWCC 2 cut(s) 148, 268
XmiI GTMKAC 1 cut(s) 106
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.