RLG00000027165

response to heat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000537
Physical Location & Seq
Forward (+)
9201859 .. 9202332
474 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027165

Sequence Viewer

Length: 474 bp
ATGTCGCTAATTCCCAACCCATTCGGCCGCCGATCGGGCGCGTTCGACCCGTTCTCTCTTGACGTGTGGGAACACCACCACCACCCACTGGACCCCTGGCCCTTCCGCTCCGTCTCGTCGCAGTCCGAAACGGCGTCGTTCGCCCACGCGCGCATCGACTGGAAGGAGACGCCGACGGCGCACGTGTTCAAGGCCGACGTGCCGGGCCTGCGGAAGGACGAGGTCAAGGTGGAGGTGGAGGACGGCAAGATCCTCCAGATCAGCGGAGAGCGGAAGCGCGATGTGGAGGAGAAGACCGACACGTGGCACCGGGTGGAGCGCAGCAGCGGCAGGTTCACGAGGCGGTTCCGGCTGCCGGAGAATGCGAGAGTCGAGCAGGTCCAGGCGGCGATGGAGAACGGCGTCCTCACCGTCACGGTGCCCAAGGAGGAGGCCAAGAAGGTCGATGTCAGGTCAATTGAGATTTCTGGTTAA

Protein Analysis

158

Amino Acids

17.99

Weight (kDa)

6.66

Isoelectric Point (pI)

54.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 53 - 156 8.2e-31 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000537)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07400 AT2G29500
fragaria_vesca FvH4_3g35270 FvH4_3g37330 FvH4_3g37360 FvH4_3g38620 FvH4_7g23650
malus_domestica MD07G1210800.v1.1 MD11G1087100.v1.1 MD11G1088300.v1.1 MD11G1089300.v1.1
prunus_persica Prupe.2G243400_v2.0.a1 Prupe.6G065900_v2.0.a1 Prupe.6G066100_v2.0.a1 Prupe.6G066200_v2.0.a1 Prupe.6G066300_v2.0.a1 Prupe.6G066400_v2.0.a1 Prupe.6G066500_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0368921 RchiOBHm_Chr1g0369001 RchiOBHm_Chr4g0444561 RchiOBHm_Chr5g0063321 RchiOBHm_Chr5g0063331 RchiOBHm_Chr5g0063361 RchiOBHm_Chr5g0067111 RchiOBHm_Chr5g0067191
rosa_laevigata RLG00000005821 RLG00000027165 RLG00000027176 RLG00000035622 RLG00000035623 RLG00000035626 RLG00000035628 RLG00000035914
rosa_multiflora Rmu_co8027810.1_g000001 Rmu_sc0000510.1_g000026 Rmu_sc0003839.1_g000004 Rmu_sc0004250.1_g000031 Rmu_sc0004250.1_g000043 Rmu_sc0005045.1_g000005 Rmu_sc0006795.1_g000002 Rmu_sc0008775.1_g000001 Rmu_sc0008775.1_g000012 Rmu_sc0012648.1_g000004
rosa_roxburghii Rroxscaffold_1G00013950 Rroxscaffold_1G00017380 Rroxscaffold_4G00288090 Rroxscaffold_4G00288200 Rroxscaffold_5G00385020
rosa_rugosa Rorug01G0345900 Rorug01G0346900 Rorug04G0353900 Rorug05G0356000 Rorug05G0356800 Rorug05G0381900 Rorug05G0382600
rosa_samantha Rh1AG353800 Rh1BG317300 Rh1CG331800 Rh1DG347900 Rh1DG348200 Rh4BG425600 Rh4CG440300 Rh4DG421200 Rh5AG415700 Rh5AG415800 Rh5AG416000 Rh5AG441900 Rh5BG430600 Rh5BG430700 Rh5BG431100 Rh5CG454100 Rh5CG454200 Rh5CG454400 Rh5DG444400 Rh5DG444500 Rh5DG444700
rosa_wichuraiana Rw1G031150 Rw1G031260 Rw4G035560 Rw5G039060 Rw5G039080 Rw5G041290 Rw5G041330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 321, 367
AccB1I GGYRCC 2 cut(s) 306, 418
AccB7I CCANNNNNTGG 1 cut(s) 88
AccBSI CCGCTC 2 cut(s) 108, 271
AccII CGCG 4 cut(s) 41, 149, 151, 279
AciI CCGC 8 cut(s) 28, 106, 211, 264, 271, 327, 343, 386
AclWI GGATC 1 cut(s) 244
AcoI YGGCCR 1 cut(s) 25
AcvI CACGTG 2 cut(s) 184, 303
AcyI GRCGYC 3 cut(s) 134, 170, 402
AdeI CACNNNGTG 1 cut(s) 313
AfiI CCNNNNNNNGG 5 cut(s) 34, 88, 214, 303, 355
AflIII ACRYGT 3 cut(s) 63, 183, 300
AgsI TTSAA 1 cut(s) 190
AjiI CACGTC 2 cut(s) 64, 199
AjnI CCWGG 2 cut(s) 95, 381
Alw26I GTCTC 2 cut(s) 118, 161
AlwI GGATC 1 cut(s) 244
AoxI GGCC 5 cut(s) 25, 98, 192, 205, 432
ApeKI GCWGC 3 cut(s) 321, 324, 352
AspLEI GCGC 6 cut(s) 41, 151, 153, 181, 279, 321
AspS9I GGNCC 4 cut(s) 91, 99, 205, 379
AsuC2I CCSGG 2 cut(s) 204, 311
AsuHPI GGTGA 1 cut(s) 400
AvaII GGWCC 2 cut(s) 91, 379
BaeGI GKGCMC 1 cut(s) 423
BanI GGYRCC 2 cut(s) 306, 418
BauI CACGAG 1 cut(s) 337
BbrPI CACGTG 2 cut(s) 184, 303
BbsI GAAGAC 1 cut(s) 299
BbvI GCAGC 3 cut(s) 333, 336, 339
BccI CCATC 1 cut(s) 385
BceAI ACGGC 4 cut(s) 147, 192, 259, 415
BciT130I CCWGG 2 cut(s) 97, 383
BcnI CCSGG 2 cut(s) 204, 311
BcoDI GTCTC 2 cut(s) 118, 161
BfuAI ACCTGC 2 cut(s) 321, 367
BglI GCCNNNNNGGC 1 cut(s) 36
BisI GCNGC 6 cut(s) 28, 322, 325, 328, 353, 387
BlsI GCNGC 6 cut(s) 29, 323, 326, 329, 354, 388
Bme1390I CCNGG 4 cut(s) 97, 204, 311, 383
Bme18I GGWCC 2 cut(s) 91, 379
BmgBI CACGTC 2 cut(s) 64, 199
BmgT120I GGNCC 4 cut(s) 91, 99, 205, 379
BmiI GGNNCC 4 cut(s) 93, 308, 347, 420
BmrFI CCNGG 4 cut(s) 97, 204, 311, 383
BmsI GCATC 1 cut(s) 162
BpiI GAAGAC 1 cut(s) 299
BpmI CTGGAG 1 cut(s) 239
BpuMI CCSGG 2 cut(s) 204, 311
BsaAI YACGTR 2 cut(s) 184, 303
BsaHI GRCGYC 3 cut(s) 134, 170, 402
BsaJI CCNNGG 2 cut(s) 95, 423
Bsc4I CCNNNNNNNGG 5 cut(s) 34, 88, 214, 303, 355
Bse1I ACTGG 2 cut(s) 93, 164
BseBI CCWGG 2 cut(s) 97, 383
BseDI CCNNGG 2 cut(s) 95, 423
BseLI CCNNNNNNNGG 5 cut(s) 34, 88, 214, 303, 355
BseNI ACTGG 2 cut(s) 93, 164
BsePI GCGCGC 1 cut(s) 149
BseRI GAGGAG 2 cut(s) 302, 443
BseSI GKGCMC 1 cut(s) 423
BseX3I CGGCCG 1 cut(s) 25
BseXI GCAGC 3 cut(s) 333, 336, 339
Bsh1236I CGCG 4 cut(s) 41, 149, 151, 279
Bsh1285I CGRYCG 2 cut(s) 28, 35
BshFI GGCC 5 cut(s) 27, 100, 194, 207, 434
BshNI GGYRCC 2 cut(s) 306, 418
BsiEI CGRYCG 2 cut(s) 28, 35
BsiSI CCGG 4 cut(s) 203, 310, 349, 356
BslI CCNNNNNNNGG 5 cut(s) 34, 88, 214, 303, 355
BsmAI GTCTC 2 cut(s) 118, 161
BsmBI CGTCTC 2 cut(s) 118, 161
BsmI GAATGC 1 cut(s) 367
BsnI GGCC 5 cut(s) 27, 100, 194, 207, 434
Bsp1286I GDGCHC 1 cut(s) 423
Bsp143I GATC 3 cut(s) 32, 249, 258
BspACI CCGC 8 cut(s) 28, 106, 211, 264, 271, 327, 343, 386
BspANI GGCC 5 cut(s) 27, 100, 194, 207, 434
BspFNI CGCG 4 cut(s) 41, 149, 151, 279
BspLI GGNNCC 4 cut(s) 93, 308, 347, 420
BspMI ACCTGC 2 cut(s) 321, 367
BspPI GGATC 1 cut(s) 244
BspT107I GGYRCC 2 cut(s) 306, 418
BsrBI CCGCTC 2 cut(s) 108, 271
BsrI ACTGG 2 cut(s) 93, 164
BssECI CCNNGG 2 cut(s) 95, 423
BssHII GCGCGC 1 cut(s) 149
BssMI GATC 3 cut(s) 32, 249, 258
BssNI GRCGYC 3 cut(s) 134, 170, 402
BssSI CACGAG 1 cut(s) 337
BssT1I CCWWGG 1 cut(s) 423
Bst2BI CACGAG 1 cut(s) 337
Bst2UI CCWGG 2 cut(s) 97, 383
Bst4CI ACNGT 2 cut(s) 412, 418
BstACI GRCGYC 3 cut(s) 134, 170, 402
BstBAI YACGTR 2 cut(s) 184, 303
BstC8I GCNNGC 2 cut(s) 151, 209
BstENI CCTNNNNNAGG 1 cut(s) 212
BstFNI CGCG 4 cut(s) 41, 149, 151, 279
BstHHI GCGC 6 cut(s) 41, 151, 153, 181, 279, 321
BstKTI GATC 3 cut(s) 35, 252, 261
BstMAI GTCTC 2 cut(s) 118, 161
BstMBI GATC 3 cut(s) 32, 249, 258
BstMCI CGRYCG 2 cut(s) 28, 35
BstMWI GCNNNNNNNGC 6 cut(s) 36, 140, 178, 208, 327, 349
BstNI CCWGG 2 cut(s) 97, 383
BstSCI CCNGG 4 cut(s) 95, 202, 309, 381
BstSLI GKGCMC 1 cut(s) 423
BstUI CGCG 4 cut(s) 41, 149, 151, 279
BstV1I GCAGC 3 cut(s) 333, 336, 339
BstV2I GAAGAC 1 cut(s) 299
BstX2I RGATCY 1 cut(s) 249
BstYI RGATCY 1 cut(s) 249
BstZI CGGCCG 1 cut(s) 25
BsuRI GGCC 5 cut(s) 27, 100, 194, 207, 434
BtgZI GCGATG 2 cut(s) 294, 404
BtrI CACGTC 2 cut(s) 64, 199
BtsIMutI CAGTG 1 cut(s) 86
BveI ACCTGC 2 cut(s) 321, 367
Cac8I GCNNGC 2 cut(s) 151, 209
CfoI GCGC 6 cut(s) 41, 151, 153, 181, 279, 321
Cfr13I GGNCC 4 cut(s) 91, 99, 205, 379
CseI GACGC 3 cut(s) 123, 178, 391
CviJI RGCY 6 cut(s) 27, 100, 194, 207, 352, 434
CviKI_1 RGCY 6 cut(s) 27, 100, 194, 207, 352, 434
DpnI GATC 3 cut(s) 34, 251, 260
DpnII GATC 3 cut(s) 32, 249, 258
DraIII CACNNNGTG 1 cut(s) 313
EaeI YGGCCR 1 cut(s) 25
EagI CGGCCG 1 cut(s) 25
EclXI CGGCCG 1 cut(s) 25
Eco130I CCWWGG 1 cut(s) 423
Eco47I GGWCC 2 cut(s) 91, 379
Eco52I CGGCCG 1 cut(s) 25
Eco72I CACGTG 2 cut(s) 184, 303
EcoNI CCTNNNNNAGG 1 cut(s) 212
EcoRII CCWGG 2 cut(s) 95, 381
EcoT14I CCWWGG 1 cut(s) 423
ErhI CCWWGG 1 cut(s) 423
Esp3I CGTCTC 2 cut(s) 118, 161
Fnu4HI GCNGC 6 cut(s) 28, 322, 325, 328, 353, 387
Fsp4HI GCNGC 6 cut(s) 28, 322, 325, 328, 353, 387
GlaI GCGC 6 cut(s) 40, 150, 152, 180, 278, 320
GluI GCNGC 6 cut(s) 28, 322, 325, 328, 353, 387
GsuI CTGGAG 1 cut(s) 239
HaeIII GGCC 5 cut(s) 27, 100, 194, 207, 434
HapII CCGG 4 cut(s) 203, 310, 349, 356
HgaI GACGC 3 cut(s) 123, 178, 391
HhaI GCGC 6 cut(s) 41, 151, 153, 181, 279, 321
Hin1I GRCGYC 3 cut(s) 134, 170, 402
Hin6I GCGC 6 cut(s) 39, 149, 151, 179, 277, 319
HinP1I GCGC 6 cut(s) 39, 149, 151, 179, 277, 319
HinfI GANTC 1 cut(s) 369
HpaII CCGG 4 cut(s) 203, 310, 349, 356
HphI GGTGA 1 cut(s) 400
Hpy166II GTNNAC 1 cut(s) 336
Hpy188I TCNGA 1 cut(s) 127
Hpy188III TCNNGA 3 cut(s) 59, 256, 337
Hpy8I GTNNAC 1 cut(s) 336
Hpy99I CGWCG 4 cut(s) 121, 139, 178, 200
HpyAV CCTTC 4 cut(s) 112, 157, 208, 433
HpyCH4III ACNGT 2 cut(s) 412, 418
HpyCH4IV ACGT 4 cut(s) 63, 183, 198, 302
HpyF10VI GCNNNNNNNGC 6 cut(s) 36, 140, 178, 208, 327, 349
HpySE526I ACGT 4 cut(s) 63, 183, 198, 302
Hsp92I GRCGYC 3 cut(s) 134, 170, 402
HspAI GCGC 6 cut(s) 39, 149, 151, 179, 277, 319
Kzo9I GATC 3 cut(s) 32, 249, 258
LmnI GCTCC 2 cut(s) 113, 316
Lsp1109I GCAGC 3 cut(s) 333, 336, 339
LweI GCATC 1 cut(s) 162
MaeII ACGT 4 cut(s) 63, 183, 198, 302
MaeIII GTNAC 1 cut(s) 412
MalI GATC 3 cut(s) 34, 251, 260
MbiI CCGCTC 2 cut(s) 108, 271
MboI GATC 3 cut(s) 32, 249, 258
MboII GAAGA 1 cut(s) 304
MfeI CAATTG 1 cut(s) 456
MflI RGATCY 1 cut(s) 249
MhlI GDGCHC 1 cut(s) 423
MluCI AATT 2 cut(s) 9, 456
MlyI GAGTC 1 cut(s) 378
MnlI CCTC 9 cut(s) 214, 226, 232, 263, 280, 333, 416, 421, 424
MseI TTAA 1 cut(s) 472
MspA1I CMGCKG 2 cut(s) 264, 327
MspI CCGG 4 cut(s) 203, 310, 349, 356
MspR9I CCNGG 4 cut(s) 97, 204, 311, 383
MunI CAATTG 1 cut(s) 456
Mva1269I GAATGC 1 cut(s) 367
MvaI CCWGG 2 cut(s) 97, 383
MvnI CGCG 4 cut(s) 41, 149, 151, 279
MwoI GCNNNNNNNGC 6 cut(s) 36, 140, 178, 208, 327, 349
NciI CCSGG 2 cut(s) 204, 311
NdeII GATC 3 cut(s) 32, 249, 258
NlaIV GGNNCC 4 cut(s) 93, 308, 347, 420
NmuCI GTSAC 1 cut(s) 412
PauI GCGCGC 1 cut(s) 149
PcsI WCGNNNNNNNCGW 1 cut(s) 153
PctI GAATGC 1 cut(s) 367
PflFI GACNNNGTC 1 cut(s) 221
PflMI CCANNNNNTGG 1 cut(s) 88
PkrI GCNGC 6 cut(s) 29, 323, 326, 329, 354, 388
Ple19I CGATCG 1 cut(s) 35
PleI GAGTC 1 cut(s) 377
PmaCI CACGTG 2 cut(s) 184, 303
PmlI CACGTG 2 cut(s) 184, 303
PpsI GAGTC 1 cut(s) 377
Ppu21I YACGTR 2 cut(s) 184, 303
Psp6I CCWGG 2 cut(s) 95, 381
PspCI CACGTG 2 cut(s) 184, 303
PspGI CCWGG 2 cut(s) 95, 381
PspN4I GGNNCC 4 cut(s) 93, 308, 347, 420
PspPI GGNCC 4 cut(s) 91, 99, 205, 379
PsuI RGATCY 1 cut(s) 249
PsyI GACNNNGTC 1 cut(s) 221
PteI GCGCGC 1 cut(s) 149
PvuI CGATCG 1 cut(s) 35
SaqAI TTAA 1 cut(s) 472
SatI GCNGC 6 cut(s) 28, 322, 325, 328, 353, 387
Sau3AI GATC 3 cut(s) 32, 249, 258
Sau96I GGNCC 4 cut(s) 91, 99, 205, 379
SchI GAGTC 1 cut(s) 378
ScrFI CCNGG 4 cut(s) 97, 204, 311, 383
SduI GDGCHC 1 cut(s) 423
SfaNI GCATC 1 cut(s) 162
SinI GGWCC 2 cut(s) 91, 379
Sse9I AATT 2 cut(s) 9, 456
SsiI CCGC 8 cut(s) 28, 106, 211, 264, 271, 327, 343, 386
StyD4I CCNGG 4 cut(s) 95, 202, 309, 381
StyI CCWWGG 1 cut(s) 423
TaaI ACNGT 2 cut(s) 412, 418
TaiI ACGT 4 cut(s) 66, 186, 201, 305
TaqI TCGA 4 cut(s) 45, 156, 372, 444
TaqII GACCGA 1 cut(s) 311
TasI AATT 2 cut(s) 9, 456
TauI GCSGC 3 cut(s) 30, 330, 389
Tru1I TTAA 1 cut(s) 472
Tru9I TTAA 1 cut(s) 472
TscAI CASTG 1 cut(s) 93
TseFI GTSAC 1 cut(s) 412
TseI GCWGC 3 cut(s) 321, 324, 352
Tsp45I GTSAC 1 cut(s) 412
TspGWI ACGGA 1 cut(s) 100
TspRI CASTG 1 cut(s) 93
Tth111I GACNNNGTC 1 cut(s) 221
Van91I CCANNNNNTGG 1 cut(s) 88
VpaK11BI GGWCC 2 cut(s) 91, 379
XagI CCTNNNNNAGG 1 cut(s) 212
XcmI CCANNNNNNNNNTGG 1 cut(s) 93
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.