Rh1DG348200

response to heat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0000537
Physical Location & Seq
Reverse (-)
57275990 .. 57276475
486 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG348200.1

Sequence Viewer

Length: 486 bp
ATGTCGCTGATTCCCAACCCATTCGGCCGCCGATTGGGCGCGTTCGACCCGTTCTCTCTTGACGTGTGGGACCACCACCACCACCACCCACTGGACCCCTGGCCCTTCCGCTCCGTCTCGTCGCAGTCCGAAACGGCGTCGTTCGCCCACGCGCACATCGACTGGAAGGAGACGCCGACGGCGCACGTGTTCAAGGCCGACGTGCCTGGCCTGCGGAAGGACGAGGTCAAGGTGGAGGTGGAGGACGGCAAGATCCTCCAGATCAGCGGAGAGCGGAAGCGCGATGTGGAGGAGAAGACCGACACGTGGCACCGGGTGGAGCGCAGGCTGCGCAGCAGCGGCAGGTTCACGAGACGGTTCCGGCTGCCGGAGAATGCGAGAGTCGAGCAGGTCCAGGCGGCGATGGAGAACGGCGTCCTCACCGTCACGGTGCCTAAGGAGGAGGCCAAGAAGGTCGATGTCAGGTCAATTGAGATCTCCGGTTAA

Protein Analysis

161

Amino Acids

18.55

Weight (kDa)

7.15

Isoelectric Point (pI)

58.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 54 - 160 1.6e-28 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000537)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07400 AT2G29500
fragaria_vesca FvH4_3g35270 FvH4_3g37330 FvH4_3g37360 FvH4_3g38620 FvH4_7g23650
malus_domestica MD07G1210800.v1.1 MD11G1087100.v1.1 MD11G1088300.v1.1 MD11G1089300.v1.1
prunus_persica Prupe.2G243400_v2.0.a1 Prupe.6G065900_v2.0.a1 Prupe.6G066100_v2.0.a1 Prupe.6G066200_v2.0.a1 Prupe.6G066300_v2.0.a1 Prupe.6G066400_v2.0.a1 Prupe.6G066500_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0368921 RchiOBHm_Chr1g0369001 RchiOBHm_Chr4g0444561 RchiOBHm_Chr5g0063321 RchiOBHm_Chr5g0063331 RchiOBHm_Chr5g0063361 RchiOBHm_Chr5g0067111 RchiOBHm_Chr5g0067191
rosa_laevigata RLG00000005821 RLG00000027165 RLG00000027176 RLG00000035622 RLG00000035623 RLG00000035626 RLG00000035628 RLG00000035914
rosa_multiflora Rmu_co8027810.1_g000001 Rmu_sc0000510.1_g000026 Rmu_sc0003839.1_g000004 Rmu_sc0004250.1_g000031 Rmu_sc0004250.1_g000043 Rmu_sc0005045.1_g000005 Rmu_sc0006795.1_g000002 Rmu_sc0008775.1_g000001 Rmu_sc0008775.1_g000012 Rmu_sc0012648.1_g000004
rosa_roxburghii Rroxscaffold_1G00013950 Rroxscaffold_1G00017380 Rroxscaffold_4G00288090 Rroxscaffold_4G00288200 Rroxscaffold_5G00385020
rosa_rugosa Rorug01G0345900 Rorug01G0346900 Rorug04G0353900 Rorug05G0356000 Rorug05G0356800 Rorug05G0381900 Rorug05G0382600
rosa_samantha Rh1AG353800 Rh1BG317300 Rh1CG331800 Rh1DG347900 Rh1DG348200 Rh4BG425600 Rh4CG440300 Rh4DG421200 Rh5AG415700 Rh5AG415800 Rh5AG416000 Rh5AG441900 Rh5BG430600 Rh5BG430700 Rh5BG431100 Rh5CG454100 Rh5CG454200 Rh5CG454400 Rh5DG444400 Rh5DG444500 Rh5DG444700
rosa_wichuraiana Rw1G031150 Rw1G031260 Rw4G035560 Rw5G039060 Rw5G039080 Rw5G041290 Rw5G041330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 332
Acc36I ACCTGC 2 cut(s) 333, 379
AccB1I GGYRCC 2 cut(s) 309, 430
AccB7I CCANNNNNTGG 1 cut(s) 91
AccBSI CCGCTC 2 cut(s) 111, 274
AccII CGCG 3 cut(s) 41, 152, 282
AciI CCGC 7 cut(s) 28, 109, 214, 267, 274, 339, 398
AclWI GGATC 1 cut(s) 247
AcoI YGGCCR 1 cut(s) 25
AcvI CACGTG 2 cut(s) 187, 306
AcyI GRCGYC 3 cut(s) 137, 173, 414
AdeI CACNNNGTG 1 cut(s) 316
AfiI CCNNNNNNNGG 5 cut(s) 34, 91, 217, 306, 367
AflIII ACRYGT 3 cut(s) 63, 186, 303
AgsI TTSAA 1 cut(s) 193
AjiI CACGTC 2 cut(s) 64, 202
AjnI CCWGG 3 cut(s) 98, 205, 393
Alw26I GTCTC 3 cut(s) 121, 164, 346
AlwI GGATC 1 cut(s) 247
AoxI GGCC 5 cut(s) 25, 101, 195, 208, 444
ApeKI GCWGC 4 cut(s) 328, 333, 336, 364
AspLEI GCGC 6 cut(s) 41, 154, 184, 282, 324, 333
AspS9I GGNCC 4 cut(s) 70, 94, 102, 391
AsuC2I CCSGG 1 cut(s) 314
AsuHPI GGTGA 1 cut(s) 412
AvaII GGWCC 3 cut(s) 70, 94, 391
AxyI CCTNAGG 1 cut(s) 435
BanI GGYRCC 2 cut(s) 309, 430
BauI CACGAG 1 cut(s) 349
BbrPI CACGTG 2 cut(s) 187, 306
BbsI GAAGAC 1 cut(s) 302
BbvI GCAGC 4 cut(s) 315, 345, 348, 351
BccI CCATC 1 cut(s) 397
BceAI ACGGC 4 cut(s) 150, 195, 262, 427
BciT130I CCWGG 3 cut(s) 100, 207, 395
BcnI CCSGG 1 cut(s) 314
BcoDI GTCTC 3 cut(s) 121, 164, 346
BfuAI ACCTGC 2 cut(s) 333, 379
BglI GCCNNNNNGGC 1 cut(s) 36
BglII AGATCT 1 cut(s) 474
BisI GCNGC 7 cut(s) 28, 329, 334, 337, 340, 365, 399
BlsI GCNGC 7 cut(s) 29, 330, 335, 338, 341, 366, 400
Bme1390I CCNGG 4 cut(s) 100, 207, 314, 395
Bme18I GGWCC 3 cut(s) 70, 94, 391
BmgBI CACGTC 2 cut(s) 64, 202
BmgT120I GGNCC 4 cut(s) 70, 94, 102, 391
BmiI GGNNCC 5 cut(s) 71, 96, 311, 359, 432
BmrFI CCNGG 4 cut(s) 100, 207, 314, 395
BpiI GAAGAC 1 cut(s) 302
BpmI CTGGAG 1 cut(s) 242
BpuMI CCSGG 1 cut(s) 314
BsaAI YACGTR 2 cut(s) 187, 306
BsaHI GRCGYC 3 cut(s) 137, 173, 414
BsaJI CCNNGG 1 cut(s) 98
BsaWI WCCGGW 1 cut(s) 479
Bsc4I CCNNNNNNNGG 5 cut(s) 34, 91, 217, 306, 367
Bse1I ACTGG 2 cut(s) 96, 167
Bse21I CCTNAGG 1 cut(s) 435
BseBI CCWGG 3 cut(s) 100, 207, 395
BseDI CCNNGG 1 cut(s) 98
BseLI CCNNNNNNNGG 5 cut(s) 34, 91, 217, 306, 367
BseNI ACTGG 2 cut(s) 96, 167
BseRI GAGGAG 2 cut(s) 305, 455
BseX3I CGGCCG 1 cut(s) 25
BseXI GCAGC 4 cut(s) 315, 345, 348, 351
Bsh1236I CGCG 3 cut(s) 41, 152, 282
Bsh1285I CGRYCG 1 cut(s) 28
BshFI GGCC 5 cut(s) 27, 103, 197, 210, 446
BshNI GGYRCC 2 cut(s) 309, 430
BsiEI CGRYCG 1 cut(s) 28
BsiSI CCGG 4 cut(s) 313, 361, 368, 480
BslFI GGGAC 1 cut(s) 83
BslI CCNNNNNNNGG 5 cut(s) 34, 91, 217, 306, 367
BsmAI GTCTC 3 cut(s) 121, 164, 346
BsmBI CGTCTC 3 cut(s) 121, 164, 346
BsmFI GGGAC 1 cut(s) 83
BsmI GAATGC 1 cut(s) 379
BsnI GGCC 5 cut(s) 27, 103, 197, 210, 446
Bsp143I GATC 3 cut(s) 252, 261, 474
BspACI CCGC 7 cut(s) 28, 109, 214, 267, 274, 339, 398
BspANI GGCC 5 cut(s) 27, 103, 197, 210, 446
BspFNI CGCG 3 cut(s) 41, 152, 282
BspLI GGNNCC 5 cut(s) 71, 96, 311, 359, 432
BspMI ACCTGC 2 cut(s) 333, 379
BspPI GGATC 1 cut(s) 247
BspT107I GGYRCC 2 cut(s) 309, 430
BsrBI CCGCTC 2 cut(s) 111, 274
BsrI ACTGG 2 cut(s) 96, 167
BssECI CCNNGG 1 cut(s) 98
BssMI GATC 3 cut(s) 252, 261, 474
BssNI GRCGYC 3 cut(s) 137, 173, 414
BssSI CACGAG 1 cut(s) 349
Bst2BI CACGAG 1 cut(s) 349
Bst2UI CCWGG 3 cut(s) 100, 207, 395
Bst4CI ACNGT 3 cut(s) 357, 424, 430
BstACI GRCGYC 3 cut(s) 137, 173, 414
BstBAI YACGTR 2 cut(s) 187, 306
BstC8I GCNNGC 2 cut(s) 212, 326
BstDEI CTNAG 1 cut(s) 435
BstENI CCTNNNNNAGG 1 cut(s) 215
BstFNI CGCG 3 cut(s) 41, 152, 282
BstHHI GCGC 6 cut(s) 41, 154, 184, 282, 324, 333
BstKTI GATC 3 cut(s) 255, 264, 477
BstMAI GTCTC 3 cut(s) 121, 164, 346
BstMBI GATC 3 cut(s) 252, 261, 474
BstMCI CGRYCG 1 cut(s) 28
BstMWI GCNNNNNNNGC 7 cut(s) 36, 143, 181, 211, 328, 330, 339
BstNI CCWGG 3 cut(s) 100, 207, 395
BstSCI CCNGG 4 cut(s) 98, 205, 312, 393
BstUI CGCG 3 cut(s) 41, 152, 282
BstV1I GCAGC 4 cut(s) 315, 345, 348, 351
BstV2I GAAGAC 1 cut(s) 302
BstX2I RGATCY 2 cut(s) 252, 474
BstYI RGATCY 2 cut(s) 252, 474
BstZI CGGCCG 1 cut(s) 25
Bsu36I CCTNAGG 1 cut(s) 435
BsuRI GGCC 5 cut(s) 27, 103, 197, 210, 446
BtgZI GCGATG 2 cut(s) 297, 416
BtrI CACGTC 2 cut(s) 64, 202
BtsIMutI CAGTG 1 cut(s) 89
BveI ACCTGC 2 cut(s) 333, 379
Cac8I GCNNGC 2 cut(s) 212, 326
CfoI GCGC 6 cut(s) 41, 154, 184, 282, 324, 333
Cfr13I GGNCC 4 cut(s) 70, 94, 102, 391
CseI GACGC 3 cut(s) 126, 181, 403
CviJI RGCY 7 cut(s) 27, 103, 197, 210, 328, 364, 446
CviKI_1 RGCY 7 cut(s) 27, 103, 197, 210, 328, 364, 446
DdeI CTNAG 1 cut(s) 435
DpnI GATC 3 cut(s) 254, 263, 476
DpnII GATC 3 cut(s) 252, 261, 474
DraIII CACNNNGTG 1 cut(s) 316
EaeI YGGCCR 1 cut(s) 25
EagI CGGCCG 1 cut(s) 25
EclXI CGGCCG 1 cut(s) 25
Eco47I GGWCC 3 cut(s) 70, 94, 391
Eco52I CGGCCG 1 cut(s) 25
Eco72I CACGTG 2 cut(s) 187, 306
Eco81I CCTNAGG 1 cut(s) 435
EcoNI CCTNNNNNAGG 1 cut(s) 215
EcoRII CCWGG 3 cut(s) 98, 205, 393
Esp3I CGTCTC 3 cut(s) 121, 164, 346
FaqI GGGAC 1 cut(s) 83
Fnu4HI GCNGC 7 cut(s) 28, 329, 334, 337, 340, 365, 399
Fsp4HI GCNGC 7 cut(s) 28, 329, 334, 337, 340, 365, 399
FspI TGCGCA 1 cut(s) 332
GlaI GCGC 6 cut(s) 40, 153, 183, 281, 323, 332
GluI GCNGC 7 cut(s) 28, 329, 334, 337, 340, 365, 399
GsuI CTGGAG 1 cut(s) 242
HaeIII GGCC 5 cut(s) 27, 103, 197, 210, 446
HapII CCGG 4 cut(s) 313, 361, 368, 480
HgaI GACGC 3 cut(s) 126, 181, 403
HhaI GCGC 6 cut(s) 41, 154, 184, 282, 324, 333
Hin1I GRCGYC 3 cut(s) 137, 173, 414
Hin6I GCGC 6 cut(s) 39, 152, 182, 280, 322, 331
HinP1I GCGC 6 cut(s) 39, 152, 182, 280, 322, 331
HinfI GANTC 2 cut(s) 10, 381
HpaII CCGG 4 cut(s) 313, 361, 368, 480
HphI GGTGA 1 cut(s) 412
Hpy166II GTNNAC 1 cut(s) 348
Hpy188I TCNGA 1 cut(s) 130
Hpy188III TCNNGA 3 cut(s) 59, 259, 349
Hpy8I GTNNAC 1 cut(s) 348
Hpy99I CGWCG 4 cut(s) 124, 142, 181, 203
HpyAV CCTTC 4 cut(s) 115, 160, 211, 445
HpyCH4III ACNGT 3 cut(s) 357, 424, 430
HpyCH4IV ACGT 4 cut(s) 63, 186, 201, 305
HpyF10VI GCNNNNNNNGC 7 cut(s) 36, 143, 181, 211, 328, 330, 339
HpyF3I CTNAG 1 cut(s) 435
HpySE526I ACGT 4 cut(s) 63, 186, 201, 305
Hsp92I GRCGYC 3 cut(s) 137, 173, 414
HspAI GCGC 6 cut(s) 39, 152, 182, 280, 322, 331
Kzo9I GATC 3 cut(s) 252, 261, 474
LmnI GCTCC 2 cut(s) 116, 319
Lsp1109I GCAGC 4 cut(s) 315, 345, 348, 351
MaeII ACGT 4 cut(s) 63, 186, 201, 305
MaeIII GTNAC 1 cut(s) 424
MalI GATC 3 cut(s) 254, 263, 476
MbiI CCGCTC 2 cut(s) 111, 274
MboI GATC 3 cut(s) 252, 261, 474
MboII GAAGA 1 cut(s) 307
MfeI CAATTG 1 cut(s) 468
MflI RGATCY 2 cut(s) 252, 474
MluCI AATT 1 cut(s) 468
MlyI GAGTC 1 cut(s) 390
MnlI CCTC 8 cut(s) 217, 229, 235, 266, 283, 428, 433, 436
MseI TTAA 1 cut(s) 484
MspA1I CMGCKG 2 cut(s) 267, 339
MspI CCGG 4 cut(s) 313, 361, 368, 480
MspR9I CCNGG 4 cut(s) 100, 207, 314, 395
MunI CAATTG 1 cut(s) 468
Mva1269I GAATGC 1 cut(s) 379
MvaI CCWGG 3 cut(s) 100, 207, 395
MvnI CGCG 3 cut(s) 41, 152, 282
MwoI GCNNNNNNNGC 7 cut(s) 36, 143, 181, 211, 328, 330, 339
NciI CCSGG 1 cut(s) 314
NdeII GATC 3 cut(s) 252, 261, 474
NlaIV GGNNCC 5 cut(s) 71, 96, 311, 359, 432
NmuCI GTSAC 1 cut(s) 424
NsbI TGCGCA 1 cut(s) 332
PcsI WCGNNNNNNNCGW 1 cut(s) 156
PctI GAATGC 1 cut(s) 379
PfeI GAWTC 1 cut(s) 10
PflFI GACNNNGTC 1 cut(s) 224
PflMI CCANNNNNTGG 1 cut(s) 91
PkrI GCNGC 7 cut(s) 29, 330, 335, 338, 341, 366, 400
PleI GAGTC 1 cut(s) 389
PmaCI CACGTG 2 cut(s) 187, 306
PmlI CACGTG 2 cut(s) 187, 306
PpsI GAGTC 1 cut(s) 389
Ppu21I YACGTR 2 cut(s) 187, 306
Psp6I CCWGG 3 cut(s) 98, 205, 393
PspCI CACGTG 2 cut(s) 187, 306
PspGI CCWGG 3 cut(s) 98, 205, 393
PspN4I GGNNCC 5 cut(s) 71, 96, 311, 359, 432
PspPI GGNCC 4 cut(s) 70, 94, 102, 391
PsuI RGATCY 2 cut(s) 252, 474
PsyI GACNNNGTC 1 cut(s) 224
SaqAI TTAA 1 cut(s) 484
SatI GCNGC 7 cut(s) 28, 329, 334, 337, 340, 365, 399
Sau3AI GATC 3 cut(s) 252, 261, 474
Sau96I GGNCC 4 cut(s) 70, 94, 102, 391
SchI GAGTC 1 cut(s) 390
ScrFI CCNGG 4 cut(s) 100, 207, 314, 395
SinI GGWCC 3 cut(s) 70, 94, 391
Sse9I AATT 1 cut(s) 468
SsiI CCGC 7 cut(s) 28, 109, 214, 267, 274, 339, 398
StyD4I CCNGG 4 cut(s) 98, 205, 312, 393
TaaI ACNGT 3 cut(s) 357, 424, 430
TaiI ACGT 4 cut(s) 66, 189, 204, 308
TaqI TCGA 4 cut(s) 45, 159, 384, 456
TaqII GACCGA 1 cut(s) 314
TasI AATT 1 cut(s) 468
TauI GCSGC 3 cut(s) 30, 342, 401
TfiI GAWTC 1 cut(s) 10
Tru1I TTAA 1 cut(s) 484
Tru9I TTAA 1 cut(s) 484
TscAI CASTG 1 cut(s) 96
TseFI GTSAC 1 cut(s) 424
TseI GCWGC 4 cut(s) 328, 333, 336, 364
Tsp45I GTSAC 1 cut(s) 424
TspGWI ACGGA 1 cut(s) 103
TspRI CASTG 1 cut(s) 96
Tth111I GACNNNGTC 1 cut(s) 224
Van91I CCANNNNNTGG 1 cut(s) 91
VpaK11BI GGWCC 3 cut(s) 70, 94, 391
XagI CCTNNNNNAGG 1 cut(s) 215
XcmI CCANNNNNNNNNTGG 1 cut(s) 96
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.