Rh5AG441900

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000537
Physical Location & Seq
Reverse (-)
75797062 .. 75801407
4346 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG441900.1

Sequence Viewer

Length: 582 bp
ATGTCGCTTATCCCCAATTTCCGACGTAGCACGGTAGCAGCAACATCTTTGACCCCCTTTTCCCTCGATCTCTGGGACCCCTTCAAGGATTTTCCATTCCCTTCTTCATCGCTCTCTCTTTTTCCCGAATTTCTTCGCGAAAATTCGGCTTTTGTCAACACTAGGATCGACTGGAAGGAGACCCCGGAAGCCCATGTTTTCAAGGCTGACATTCCGGGGCTGACGAAAGAAGAGATCAAGGTTGAGGTAGAAGACGACAGGGTGCTTCGGATCAGCGGAGAGAGGAAGATAGAGAAGGAGGACAAGAACGACACCTGGCACCGGGTCGAGAGAAGCAGCGGCAAGTTCTCCAGAAGGTTCCTGCTTCCTGAGAATGCCAAGATGGATGAAATCAAGGCTGCTATGGAGAACGGGGTTCTCAGCGTGACTGTTCCAAAGGCGGAGGTGAAAAGGCCTGATGTTAAAGCCATTCAAATCTCTGAGGCTATATTCTGTTCTCACAACTTTTATATGGCATTTGAAATCTACAGAGGCTTGTACGAATTTCAGAAAAGCAAACACCAAATATACACTGCAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

193

Amino Acids

22.41

Weight (kDa)

6.93

Isoelectric Point (pI)

48.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 57 - 160 1.4e-31 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000537)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07400 AT2G29500
fragaria_vesca FvH4_3g35270 FvH4_3g37330 FvH4_3g37360 FvH4_3g38620 FvH4_7g23650
malus_domestica MD07G1210800.v1.1 MD11G1087100.v1.1 MD11G1088300.v1.1 MD11G1089300.v1.1
prunus_persica Prupe.2G243400_v2.0.a1 Prupe.6G065900_v2.0.a1 Prupe.6G066100_v2.0.a1 Prupe.6G066200_v2.0.a1 Prupe.6G066300_v2.0.a1 Prupe.6G066400_v2.0.a1 Prupe.6G066500_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0368921 RchiOBHm_Chr1g0369001 RchiOBHm_Chr4g0444561 RchiOBHm_Chr5g0063321 RchiOBHm_Chr5g0063331 RchiOBHm_Chr5g0063361 RchiOBHm_Chr5g0067111 RchiOBHm_Chr5g0067191
rosa_laevigata RLG00000005821 RLG00000027165 RLG00000027176 RLG00000035622 RLG00000035623 RLG00000035626 RLG00000035628 RLG00000035914
rosa_multiflora Rmu_co8027810.1_g000001 Rmu_sc0000510.1_g000026 Rmu_sc0003839.1_g000004 Rmu_sc0004250.1_g000031 Rmu_sc0004250.1_g000043 Rmu_sc0005045.1_g000005 Rmu_sc0006795.1_g000002 Rmu_sc0008775.1_g000001 Rmu_sc0008775.1_g000012 Rmu_sc0012648.1_g000004
rosa_roxburghii Rroxscaffold_1G00013950 Rroxscaffold_1G00017380 Rroxscaffold_4G00288090 Rroxscaffold_4G00288200 Rroxscaffold_5G00385020
rosa_rugosa Rorug01G0345900 Rorug01G0346900 Rorug04G0353900 Rorug05G0356000 Rorug05G0356800 Rorug05G0381900 Rorug05G0382600
rosa_samantha Rh1AG353800 Rh1BG317300 Rh1CG331800 Rh1DG347900 Rh1DG348200 Rh4BG425600 Rh4CG440300 Rh4DG421200 Rh5AG415700 Rh5AG415800 Rh5AG416000 Rh5AG441900 Rh5BG430600 Rh5BG430700 Rh5BG431100 Rh5CG454100 Rh5CG454200 Rh5CG454400 Rh5DG444400 Rh5DG444500 Rh5DG444700
rosa_wichuraiana Rw1G031150 Rw1G031260 Rw4G035560 Rw5G039060 Rw5G039080 Rw5G041290 Rw5G041330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 318
AccII CGCG 1 cut(s) 138
AciI CCGC 3 cut(s) 276, 339, 440
AclWI GGATC 2 cut(s) 173, 278
AcsI RAATTY 3 cut(s) 128, 142, 542
AfaI GTAC 1 cut(s) 539
AfiI CCNNNNNNNGG 2 cut(s) 85, 321
AgsI TTSAA 4 cut(s) 85, 202, 473, 521
AjnI CCWGG 1 cut(s) 314
Alw26I GTCTC 1 cut(s) 173
AlwI GGATC 2 cut(s) 173, 278
AoxI GGCC 1 cut(s) 452
ApeKI GCWGC 3 cut(s) 38, 336, 398
ApoI RAATTY 3 cut(s) 128, 142, 542
Asp700I GAANNNNTTC 1 cut(s) 132
AspS9I GGNCC 1 cut(s) 76
AsuC2I CCSGG 3 cut(s) 185, 216, 323
AsuHPI GGTGA 1 cut(s) 457
AvaII GGWCC 1 cut(s) 76
BanI GGYRCC 1 cut(s) 318
BbsI GAAGAC 1 cut(s) 258
BbvI GCAGC 3 cut(s) 50, 348, 385
BccI CCATC 1 cut(s) 376
BciT130I CCWGG 1 cut(s) 316
BcnI CCSGG 3 cut(s) 185, 216, 323
BcoDI GTCTC 1 cut(s) 173
BfaI CTAG 1 cut(s) 162
BfmI CTRYAG 1 cut(s) 526
BisI GCNGC 4 cut(s) 39, 337, 340, 399
BlsI GCNGC 4 cut(s) 40, 338, 341, 400
Bme1390I CCNGG 4 cut(s) 185, 216, 316, 323
Bme18I GGWCC 1 cut(s) 76
BmgT120I GGNCC 1 cut(s) 76
BmiI GGNNCC 4 cut(s) 77, 78, 320, 359
BmrFI CCNGG 4 cut(s) 185, 216, 316, 323
BpiI GAAGAC 1 cut(s) 258
BpmI CTGGAG 1 cut(s) 334
BpuMI CCSGG 3 cut(s) 185, 216, 323
BsaI GGTCTC 1 cut(s) 173
BsaJI CCNNGG 2 cut(s) 183, 215
Bsc4I CCNNNNNNNGG 2 cut(s) 85, 321
Bse1I ACTGG 1 cut(s) 176
BseBI CCWGG 1 cut(s) 316
BseDI CCNNGG 2 cut(s) 183, 215
BseGI GGATG 1 cut(s) 391
BseLI CCNNNNNNNGG 2 cut(s) 85, 321
BseMII CTCAG 3 cut(s) 360, 433, 471
BseNI ACTGG 1 cut(s) 176
BseXI GCAGC 3 cut(s) 50, 348, 385
Bsh1236I CGCG 1 cut(s) 138
BshFI GGCC 1 cut(s) 454
BshNI GGYRCC 1 cut(s) 318
BsiSI CCGG 3 cut(s) 185, 215, 322
BslFI GGGAC 1 cut(s) 89
BslI CCNNNNNNNGG 2 cut(s) 85, 321
BsmAI GTCTC 1 cut(s) 173
BsmFI GGGAC 1 cut(s) 89
BsmI GAATGC 1 cut(s) 379
BsnI GGCC 1 cut(s) 454
Bso31I GGTCTC 1 cut(s) 173
Bsp143I GATC 4 cut(s) 67, 165, 234, 270
Bsp68I TCGCGA 1 cut(s) 138
BspACI CCGC 3 cut(s) 276, 339, 440
BspANI GGCC 1 cut(s) 454
BspCNI CTCAG 3 cut(s) 361, 432, 472
BspFNI CGCG 1 cut(s) 138
BspLI GGNNCC 4 cut(s) 77, 78, 320, 359
BspPI GGATC 2 cut(s) 173, 278
BspT107I GGYRCC 1 cut(s) 318
BspTNI GGTCTC 1 cut(s) 173
BsrI ACTGG 1 cut(s) 176
BssECI CCNNGG 2 cut(s) 183, 215
BssMI GATC 4 cut(s) 67, 165, 234, 270
Bst2UI CCWGG 1 cut(s) 316
Bst4CI ACNGT 2 cut(s) 34, 430
Bst6I CTCTTC 1 cut(s) 225
BstDEI CTNAG 3 cut(s) 369, 419, 480
BstF5I GGATG 1 cut(s) 391
BstFNI CGCG 1 cut(s) 138
BstKTI GATC 4 cut(s) 70, 168, 237, 273
BstMAI GTCTC 1 cut(s) 173
BstMBI GATC 4 cut(s) 67, 165, 234, 270
BstNI CCWGG 1 cut(s) 316
BstSCI CCNGG 4 cut(s) 183, 214, 314, 321
BstSFI CTRYAG 1 cut(s) 526
BstUI CGCG 1 cut(s) 138
BstV1I GCAGC 3 cut(s) 50, 348, 385
BstV2I GAAGAC 1 cut(s) 258
BsuRI GGCC 1 cut(s) 454
BtgZI GCGATG 1 cut(s) 93
BtsCI GGATG 1 cut(s) 391
BtsI GCAGTG 1 cut(s) 570
BtsIMutI CAGTG 1 cut(s) 570
BtuMI TCGCGA 1 cut(s) 138
Cfr13I GGNCC 1 cut(s) 76
Csp6I GTAC 1 cut(s) 538
CviAII CATG 1 cut(s) 194
CviJI RGCY 9 cut(s) 149, 191, 206, 220, 398, 454, 467, 485, 534
CviKI_1 RGCY 9 cut(s) 149, 191, 206, 220, 398, 454, 467, 485, 534
CviQI GTAC 1 cut(s) 538
DdeI CTNAG 3 cut(s) 369, 419, 480
DpnI GATC 4 cut(s) 69, 167, 236, 272
DpnII GATC 4 cut(s) 67, 165, 234, 270
Eam1104I CTCTTC 1 cut(s) 225
EarI CTCTTC 1 cut(s) 225
EciI GGCGGA 1 cut(s) 455
Eco147I AGGCCT 1 cut(s) 454
Eco31I GGTCTC 1 cut(s) 173
Eco47I GGWCC 1 cut(s) 76
EcoO109I RGGNCCY 1 cut(s) 76
EcoRII CCWGG 1 cut(s) 314
FaeI CATG 1 cut(s) 197
FaiI YATR 6 cut(s) 195, 404, 488, 510, 512, 568
FaqI GGGAC 1 cut(s) 89
FatI CATG 1 cut(s) 193
Fnu4HI GCNGC 4 cut(s) 39, 337, 340, 399
FokI GGATG 1 cut(s) 398
Fsp4HI GCNGC 4 cut(s) 39, 337, 340, 399
FspBI CTAG 1 cut(s) 162
GluI GCNGC 4 cut(s) 39, 337, 340, 399
GsuI CTGGAG 1 cut(s) 334
HaeIII GGCC 1 cut(s) 454
HapII CCGG 3 cut(s) 185, 215, 322
Hin1II CATG 1 cut(s) 197
HincII GTYRAC 1 cut(s) 157
HindII GTYRAC 1 cut(s) 157
HpaII CCGG 3 cut(s) 185, 215, 322
HphI GGTGA 1 cut(s) 457
Hpy166II GTNNAC 1 cut(s) 157
Hpy188I TCNGA 4 cut(s) 23, 270, 481, 549
Hpy188III TCNNGA 5 cut(s) 125, 137, 328, 351, 368
Hpy8I GTNNAC 1 cut(s) 157
Hpy99I CGWCG 1 cut(s) 27
HpyAV CCTTC 5 cut(s) 91, 111, 169, 289, 348
HpyCH4III ACNGT 2 cut(s) 34, 430
HpyCH4IV ACGT 1 cut(s) 25
HpyCH4V TGCA 1 cut(s) 575
HpyF3I CTNAG 3 cut(s) 369, 419, 480
HpySE526I ACGT 1 cut(s) 25
Hsp92II CATG 1 cut(s) 197
KflI GGGWCCC 1 cut(s) 76
Kzo9I GATC 4 cut(s) 67, 165, 234, 270
Lsp1109I GCAGC 3 cut(s) 50, 348, 385
MaeI CTAG 1 cut(s) 162
MaeII ACGT 1 cut(s) 25
MaeIII GTNAC 1 cut(s) 424
MalI GATC 4 cut(s) 69, 167, 236, 272
MboI GATC 4 cut(s) 67, 165, 234, 270
MboII GAAGA 5 cut(s) 96, 125, 242, 263, 298
MluCI AATT 4 cut(s) 16, 128, 142, 542
MmeI TCCRAC 1 cut(s) 46
MnlI CCTC 7 cut(s) 74, 238, 276, 292, 436, 475, 524
MroXI GAANNNNTTC 1 cut(s) 132
MseI TTAA 1 cut(s) 462
MspA1I CMGCKG 2 cut(s) 276, 339
MspI CCGG 3 cut(s) 185, 215, 322
MspR9I CCNGG 4 cut(s) 185, 216, 316, 323
Mva1269I GAATGC 1 cut(s) 379
MvaI CCWGG 1 cut(s) 316
MvnI CGCG 1 cut(s) 138
NciI CCSGG 3 cut(s) 185, 216, 323
NdeII GATC 4 cut(s) 67, 165, 234, 270
NlaIII CATG 1 cut(s) 197
NlaIV GGNNCC 4 cut(s) 77, 78, 320, 359
NmuCI GTSAC 1 cut(s) 424
NruI TCGCGA 1 cut(s) 138
PceI AGGCCT 1 cut(s) 454
PctI GAATGC 1 cut(s) 379
PdmI GAANNNNTTC 1 cut(s) 132
PkrI GCNGC 4 cut(s) 40, 338, 341, 400
PpuMI RGGWCCY 1 cut(s) 76
Psp5II RGGWCCY 1 cut(s) 76
Psp6I CCWGG 1 cut(s) 314
PspGI CCWGG 1 cut(s) 314
PspN4I GGNNCC 4 cut(s) 77, 78, 320, 359
PspPI GGNCC 1 cut(s) 76
PspPPI RGGWCCY 1 cut(s) 76
RruI TCGCGA 1 cut(s) 138
RsaI GTAC 1 cut(s) 539
RsaNI GTAC 1 cut(s) 538
SaqAI TTAA 1 cut(s) 462
SatI GCNGC 4 cut(s) 39, 337, 340, 399
Sau3AI GATC 4 cut(s) 67, 165, 234, 270
Sau96I GGNCC 1 cut(s) 76
ScrFI CCNGG 4 cut(s) 185, 216, 316, 323
SetI ASST 6 cut(s) 28, 243, 249, 317, 359, 447
SfcI CTRYAG 1 cut(s) 526
SinI GGWCC 1 cut(s) 76
Sse9I AATT 4 cut(s) 16, 128, 142, 542
SseBI AGGCCT 1 cut(s) 454
SsiI CCGC 3 cut(s) 276, 339, 440
SspMI CTAG 1 cut(s) 162
StuI AGGCCT 1 cut(s) 454
StyD4I CCNGG 4 cut(s) 183, 214, 314, 321
TaaI ACNGT 2 cut(s) 34, 430
TaiI ACGT 1 cut(s) 28
TaqI TCGA 3 cut(s) 66, 168, 327
TasI AATT 4 cut(s) 16, 128, 142, 542
TauI GCSGC 1 cut(s) 342
Tru1I TTAA 1 cut(s) 462
Tru9I TTAA 1 cut(s) 462
TscAI CASTG 1 cut(s) 577
TseFI GTSAC 1 cut(s) 424
TseI GCWGC 3 cut(s) 38, 336, 398
Tsp45I GTSAC 1 cut(s) 424
TspDTI ATGAA 2 cut(s) 96, 402
TspRI CASTG 1 cut(s) 577
VpaK11BI GGWCC 1 cut(s) 76
XapI RAATTY 3 cut(s) 128, 142, 542
XmnI GAANNNNTTC 1 cut(s) 132
XspI CTAG 1 cut(s) 162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.