FvH4_3g35270

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Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000537
Physical Location & Seq
Forward (+)
30419450 .. 30420384
935 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g35270.t1

Sequence Viewer

Length: 456 bp
ATGTCGCTCATCCCAAGTTCCCGACGAAGCAGCATCTTCGACCTCGATCCCTGGGACCCATTCAGGGACATCCAATTCCCTTCCTCATTGCTCTCTACATTCCCTAGCGAAAACTCGGCTTTTGTAAACACCAGGATCGACTGGAAGGAGACCCCAGAAGCCCATGTGTTCAAGGCCGACATTCCGGGGCTGAAGAAAGAAGAGGTGAAGGTTGAGATTGAAAATGACAGGGTGCTGCAGATCAGCGGAGAGAGGAAGATAGAGAAGGAGGACAAGAACGACAAGTGGCACCGGGTCGAGAGAAGCAGCGGCAAGTTCTCCAGGAGGTTCAGGCTTCCTGAGAATGCCAAGGTTGATGAGATTAAGGCTGCAATGGAGAATGGGGTTCTGAGTGTCACTATTCCTAAGGTGGAGGTGAAGAATCCTGATGTCAAAGCCGTTGAAATTTCTGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

152

Amino Acids

17.31

Weight (kDa)

6.19

Isoelectric Point (pI)

51.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 47 - 150 5.1e-31 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000537)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07400 AT2G29500
fragaria_vesca FvH4_3g35270 FvH4_3g37330 FvH4_3g37360 FvH4_3g38620 FvH4_7g23650
malus_domestica MD07G1210800.v1.1 MD11G1087100.v1.1 MD11G1088300.v1.1 MD11G1089300.v1.1
prunus_persica Prupe.2G243400_v2.0.a1 Prupe.6G065900_v2.0.a1 Prupe.6G066100_v2.0.a1 Prupe.6G066200_v2.0.a1 Prupe.6G066300_v2.0.a1 Prupe.6G066400_v2.0.a1 Prupe.6G066500_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0368921 RchiOBHm_Chr1g0369001 RchiOBHm_Chr4g0444561 RchiOBHm_Chr5g0063321 RchiOBHm_Chr5g0063331 RchiOBHm_Chr5g0063361 RchiOBHm_Chr5g0067111 RchiOBHm_Chr5g0067191
rosa_laevigata RLG00000005821 RLG00000027165 RLG00000027176 RLG00000035622 RLG00000035623 RLG00000035626 RLG00000035628 RLG00000035914
rosa_multiflora Rmu_co8027810.1_g000001 Rmu_sc0000510.1_g000026 Rmu_sc0003839.1_g000004 Rmu_sc0004250.1_g000031 Rmu_sc0004250.1_g000043 Rmu_sc0005045.1_g000005 Rmu_sc0006795.1_g000002 Rmu_sc0008775.1_g000001 Rmu_sc0008775.1_g000012 Rmu_sc0012648.1_g000004
rosa_roxburghii Rroxscaffold_1G00013950 Rroxscaffold_1G00017380 Rroxscaffold_4G00288090 Rroxscaffold_4G00288200 Rroxscaffold_5G00385020
rosa_rugosa Rorug01G0345900 Rorug01G0346900 Rorug04G0353900 Rorug05G0356000 Rorug05G0356800 Rorug05G0381900 Rorug05G0382600
rosa_samantha Rh1AG353800 Rh1BG317300 Rh1CG331800 Rh1DG347900 Rh1DG348200 Rh4BG425600 Rh4CG440300 Rh4DG421200 Rh5AG415700 Rh5AG415800 Rh5AG416000 Rh5AG441900 Rh5BG430600 Rh5BG430700 Rh5BG431100 Rh5CG454100 Rh5CG454200 Rh5CG454400 Rh5DG444400 Rh5DG444500 Rh5DG444700
rosa_wichuraiana Rw1G031150 Rw1G031260 Rw4G035560 Rw5G039060 Rw5G039080 Rw5G041290 Rw5G041330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 288
AciI CCGC 2 cut(s) 246, 309
AclWI GGATC 2 cut(s) 41, 143
AcsI RAATTY 1 cut(s) 444
AcuI CTGAAG 1 cut(s) 212
AfiI CCNNNNNNNGG 1 cut(s) 64
AgsI TTSAA 3 cut(s) 172, 221, 443
AjnI CCWGG 3 cut(s) 50, 131, 320
Alw26I GTCTC 1 cut(s) 143
AlwI GGATC 2 cut(s) 41, 143
AoxI GGCC 1 cut(s) 174
ApeKI GCWGC 4 cut(s) 30, 235, 306, 368
ApoI RAATTY 1 cut(s) 444
AspS9I GGNCC 1 cut(s) 55
AsuC2I CCSGG 2 cut(s) 186, 293
AsuHPI GGTGA 2 cut(s) 217, 427
AvaII GGWCC 1 cut(s) 55
AxyI CCTNAGG 1 cut(s) 405
BanI GGYRCC 1 cut(s) 288
BbvI GCAGC 4 cut(s) 42, 222, 318, 355
BceAI ACGGC 1 cut(s) 422
BcgI CGANNNNNNTGC 2 cut(s) 19, 53
BciT130I CCWGG 3 cut(s) 52, 133, 322
BcnI CCSGG 2 cut(s) 186, 293
BcoDI GTCTC 1 cut(s) 143
BfaI CTAG 1 cut(s) 105
BfmI CTRYAG 1 cut(s) 236
BisI GCNGC 5 cut(s) 31, 236, 307, 310, 369
BlsI GCNGC 5 cut(s) 32, 237, 308, 311, 370
Bme1390I CCNGG 5 cut(s) 52, 133, 186, 293, 322
Bme18I GGWCC 1 cut(s) 55
BmgT120I GGNCC 1 cut(s) 55
BmiI GGNNCC 3 cut(s) 56, 57, 290
BmrFI CCNGG 5 cut(s) 52, 133, 186, 293, 322
BmsI GCATC 1 cut(s) 42
BpmI CTGGAG 1 cut(s) 304
BpuMI CCSGG 2 cut(s) 186, 293
BsaI GGTCTC 1 cut(s) 143
BsaJI CCNNGG 4 cut(s) 50, 51, 185, 348
Bsc4I CCNNNNNNNGG 1 cut(s) 64
Bse1I ACTGG 1 cut(s) 146
Bse21I CCTNAGG 1 cut(s) 405
Bse3DI GCAATG 2 cut(s) 86, 378
BseBI CCWGG 3 cut(s) 52, 133, 322
BseDI CCNNGG 4 cut(s) 50, 51, 185, 348
BseGI GGATG 2 cut(s) 9, 69
BseLI CCNNNNNNNGG 1 cut(s) 64
BseMI GCAATG 2 cut(s) 86, 378
BseMII CTCAG 2 cut(s) 330, 380
BseNI ACTGG 1 cut(s) 146
BseXI GCAGC 4 cut(s) 42, 222, 318, 355
BshFI GGCC 1 cut(s) 176
BshNI GGYRCC 1 cut(s) 288
BsiSI CCGG 2 cut(s) 185, 292
BslFI GGGAC 2 cut(s) 68, 80
BslI CCNNNNNNNGG 1 cut(s) 64
BsmAI GTCTC 1 cut(s) 143
BsmFI GGGAC 2 cut(s) 68, 80
BsmI GAATGC 1 cut(s) 349
BsnI GGCC 1 cut(s) 176
Bso31I GGTCTC 1 cut(s) 143
Bsp143I GATC 3 cut(s) 46, 135, 240
BspACI CCGC 2 cut(s) 246, 309
BspANI GGCC 1 cut(s) 176
BspCNI CTCAG 2 cut(s) 331, 381
BspLI GGNNCC 3 cut(s) 56, 57, 290
BspMAI CTGCAG 1 cut(s) 240
BspPI GGATC 2 cut(s) 41, 143
BspT107I GGYRCC 1 cut(s) 288
BspTNI GGTCTC 1 cut(s) 143
BsrDI GCAATG 2 cut(s) 86, 378
BsrI ACTGG 1 cut(s) 146
BssECI CCNNGG 4 cut(s) 50, 51, 185, 348
BssMI GATC 3 cut(s) 46, 135, 240
BssT1I CCWWGG 1 cut(s) 348
Bst2UI CCWGG 3 cut(s) 52, 133, 322
Bst6I CTCTTC 1 cut(s) 195
BstDEI CTNAG 3 cut(s) 339, 389, 405
BstF5I GGATG 2 cut(s) 9, 69
BstKTI GATC 3 cut(s) 49, 138, 243
BstMAI GTCTC 1 cut(s) 143
BstMBI GATC 3 cut(s) 46, 135, 240
BstNI CCWGG 3 cut(s) 52, 133, 322
BstSCI CCNGG 5 cut(s) 50, 131, 184, 291, 320
BstSFI CTRYAG 1 cut(s) 236
BstV1I GCAGC 4 cut(s) 42, 222, 318, 355
Bsu36I CCTNAGG 1 cut(s) 405
BsuRI GGCC 1 cut(s) 176
BtsCI GGATG 2 cut(s) 9, 69
Cfr13I GGNCC 1 cut(s) 55
CviAII CATG 1 cut(s) 164
CviJI RGCY 7 cut(s) 119, 161, 176, 190, 334, 368, 437
CviKI_1 RGCY 7 cut(s) 119, 161, 176, 190, 334, 368, 437
DdeI CTNAG 3 cut(s) 339, 389, 405
DpnI GATC 3 cut(s) 48, 137, 242
DpnII GATC 3 cut(s) 46, 135, 240
Eam1104I CTCTTC 1 cut(s) 195
EarI CTCTTC 1 cut(s) 195
Eco130I CCWWGG 1 cut(s) 348
Eco31I GGTCTC 1 cut(s) 143
Eco47I GGWCC 1 cut(s) 55
Eco57I CTGAAG 1 cut(s) 212
Eco81I CCTNAGG 1 cut(s) 405
EcoO109I RGGNCCY 1 cut(s) 55
EcoRII CCWGG 3 cut(s) 50, 131, 320
EcoT14I CCWWGG 1 cut(s) 348
ErhI CCWWGG 1 cut(s) 348
FaeI CATG 1 cut(s) 167
FaiI YATR 1 cut(s) 165
FaqI GGGAC 2 cut(s) 68, 80
FatI CATG 1 cut(s) 163
Fnu4HI GCNGC 5 cut(s) 31, 236, 307, 310, 369
FokI GGATG 1 cut(s) 56
Fsp4HI GCNGC 5 cut(s) 31, 236, 307, 310, 369
FspBI CTAG 1 cut(s) 105
GluI GCNGC 5 cut(s) 31, 236, 307, 310, 369
GsuI CTGGAG 1 cut(s) 304
HaeIII GGCC 1 cut(s) 176
HapII CCGG 2 cut(s) 185, 292
Hin1II CATG 1 cut(s) 167
HinfI GANTC 1 cut(s) 421
HpaII CCGG 2 cut(s) 185, 292
HphI GGTGA 2 cut(s) 217, 427
Hpy166II GTNNAC 1 cut(s) 127
Hpy188I TCNGA 1 cut(s) 390
Hpy188III TCNNGA 4 cut(s) 21, 298, 338, 425
Hpy8I GTNNAC 1 cut(s) 127
Hpy99I CGWCG 1 cut(s) 27
HpyAV CCTTC 4 cut(s) 90, 139, 202, 259
HpyCH4V TGCA 2 cut(s) 238, 371
HpyF3I CTNAG 3 cut(s) 339, 389, 405
Hsp92II CATG 1 cut(s) 167
KflI GGGWCCC 1 cut(s) 55
Kzo9I GATC 3 cut(s) 46, 135, 240
Lsp1109I GCAGC 4 cut(s) 42, 222, 318, 355
LweI GCATC 1 cut(s) 42
MaeI CTAG 1 cut(s) 105
MaeIII GTNAC 1 cut(s) 394
MalI GATC 3 cut(s) 48, 137, 242
MboI GATC 3 cut(s) 46, 135, 240
MboII GAAGA 5 cut(s) 28, 205, 212, 268, 430
MluCI AATT 2 cut(s) 74, 444
MnlI CCTC 7 cut(s) 53, 94, 196, 246, 262, 318, 406
MseI TTAA 1 cut(s) 363
MspA1I CMGCKG 2 cut(s) 246, 309
MspI CCGG 2 cut(s) 185, 292
MspR9I CCNGG 5 cut(s) 52, 133, 186, 293, 322
Mva1269I GAATGC 1 cut(s) 349
MvaI CCWGG 3 cut(s) 52, 133, 322
NciI CCSGG 2 cut(s) 186, 293
NdeII GATC 3 cut(s) 46, 135, 240
NlaIII CATG 1 cut(s) 167
NlaIV GGNNCC 3 cut(s) 56, 57, 290
NmeAIII GCCGAG 1 cut(s) 95
NmuCI GTSAC 1 cut(s) 394
PasI CCCWGGG 1 cut(s) 51
PctI GAATGC 1 cut(s) 349
PfeI GAWTC 1 cut(s) 421
PfoI TCCNGGA 1 cut(s) 320
PkrI GCNGC 5 cut(s) 32, 237, 308, 311, 370
PpuMI RGGWCCY 1 cut(s) 55
Psp5II RGGWCCY 1 cut(s) 55
Psp6I CCWGG 3 cut(s) 50, 131, 320
PspGI CCWGG 3 cut(s) 50, 131, 320
PspN4I GGNNCC 3 cut(s) 56, 57, 290
PspPI GGNCC 1 cut(s) 55
PspPPI RGGWCCY 1 cut(s) 55
PstI CTGCAG 1 cut(s) 240
SaqAI TTAA 1 cut(s) 363
SatI GCNGC 5 cut(s) 31, 236, 307, 310, 369
Sau3AI GATC 3 cut(s) 46, 135, 240
Sau96I GGNCC 1 cut(s) 55
ScrFI CCNGG 5 cut(s) 52, 133, 186, 293, 322
SetI ASST 7 cut(s) 45, 207, 213, 329, 354, 411, 417
SfaNI GCATC 1 cut(s) 42
SfcI CTRYAG 1 cut(s) 236
SinI GGWCC 1 cut(s) 55
Sse9I AATT 2 cut(s) 74, 444
SsiI CCGC 2 cut(s) 246, 309
SspMI CTAG 1 cut(s) 105
StyD4I CCNGG 5 cut(s) 50, 131, 184, 291, 320
StyI CCWWGG 1 cut(s) 348
TaqI TCGA 4 cut(s) 39, 45, 138, 297
TasI AATT 2 cut(s) 74, 444
TauI GCSGC 1 cut(s) 312
TfiI GAWTC 1 cut(s) 421
Tru1I TTAA 1 cut(s) 363
Tru9I TTAA 1 cut(s) 363
TseFI GTSAC 1 cut(s) 394
TseI GCWGC 4 cut(s) 30, 235, 306, 368
Tsp45I GTSAC 1 cut(s) 394
VpaK11BI GGWCC 1 cut(s) 55
XapI RAATTY 1 cut(s) 444
XspI CTAG 1 cut(s) 105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.