AT1G58430

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0001786
Physical Location & Seq
Reverse (-)
21711494 .. 21713103
1610 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G58430.1

Sequence Viewer

Length: 1083 bp
ATGTGGACTTCTAAAACCATAAGCTTCACTCTCTTCATCACAACAACACTTCTCGGGTCCTGCAACGCATCTGCAAAGGCCAAAACGCAACCGCTATTCCCAGCGATTCTAATCTTTGGTGATTCAACAGTCGACACAGGCAACAATAATTACCCTTCACAAACAATCTTCAGAGCTAAACATGTTCCTTACGGAATTGATCTCCCAAACCACTCACCTAACGGAAGATTCTCAAACGGGAAAATTTTCTCCGACATAATCGCAACCAAACTCAACATCAAACAGTTTGTTCCTCCTTTCTTACAACCAAATCTCACCGACCAAGAAATTGTAACCGGAGTCTGTTTCGCATCAGCAGGTGCCGGTTACGATGACCAAACCAGTCTCACGACACAAGCGATTCGTGTCTCGGAACAACCAAATATGTTCAAGAGTTACATTGCTCGTCTTAAGAGTATCGTAGGAGACAAGAAAGCCATGAAGATCATAAACAATGCTTTGGTGGTTGTGAGTGCAGGGCCTAATGATTTCATCTTGAATTATTACGAGGTTCCCTCATGGCGTCGCATGTATCCTAGCATTTCTGATTACCAAGATTTTGTTCTTAGTAGGCTTAACAATTTCGTGAAGGAGCTTTACAGCCTAGGTTGCCGGAAAATTTTGGTCGGAGGTTTACCGCCAATGGGATGTTTACCGATTCAAATGACTGCTCAATTCCGCAACGTCCTAAGGTTTTGCTTGGAACAAGAGAACAGAGACTCTGTTTTATACAATCAGAAACTTCAGAAGCTCTTACCTCAGACACAAGCATCTCTTACAGGAAGCAAGATCCTTTACTCTGATGTCTATGACCCTATGATGGAGATGCTCCAAAACCCTAGCAAATACGGATTTAAAGAGACGACGAGAGGATGTTGTGGAACAGGGTTCTTGGAGACGAGCTTCATGTGTAATGCTTATTCTTCCATGTGTCAGAATCGCTCGGAGTTTCTGTTCTTTGACTCGATTCATCCATCTGAAGCTACCTACAATTACATTGGTAATGTTCTGGATACTAAGATTCGTGGGTGGCTTAAGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

360

Amino Acids

40.4

Weight (kDa)

8.93

Isoelectric Point (pI)

33.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 36 - 348 5.1e-31 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 347
Acc36I ACCTGC 1 cut(s) 347
AccB1I GGYRCC 1 cut(s) 359
AccI GTMKAC 1 cut(s) 132
AciI CCGC 3 cut(s) 92, 677, 718
AclWI GGATC 1 cut(s) 823
AcsI RAATTY 2 cut(s) 243, 657
AcuI CTGAAG 3 cut(s) 154, 767, 1038
AcyI GRCGYC 1 cut(s) 562
AfiI CCNNNNNNNGG 2 cut(s) 683, 859
AflII CTTAAG 2 cut(s) 449, 1073
AflIII ACRYGT 1 cut(s) 181
AgsI TTSAA 4 cut(s) 126, 430, 538, 701
AjuI GAANNNNNNNTTGG 2 cut(s) 585, 617
AloI GAACNNNNNNTCC 2 cut(s) 977, 1009
AluBI AGCT 6 cut(s) 24, 176, 634, 790, 942, 1022
AluI AGCT 6 cut(s) 24, 176, 634, 790, 942, 1022
Alw26I GTCTC 6 cut(s) 389, 412, 459, 750, 893, 929
AlwI GGATC 1 cut(s) 823
Ama87I CYCGRG 1 cut(s) 53
AoxI GGCC 2 cut(s) 78, 518
ApoI RAATTY 2 cut(s) 243, 657
Asp700I GAANNNNTTC 1 cut(s) 245
AspA2I CCTAGG 1 cut(s) 643
AspS9I GGNCC 2 cut(s) 57, 518
AsuHPI GGTGA 3 cut(s) 131, 207, 307
AvaI CYCGRG 1 cut(s) 53
AvaII GGWCC 1 cut(s) 57
AvrII CCTAGG 1 cut(s) 643
AxyI CCTNAGG 1 cut(s) 728
BanI GGYRCC 1 cut(s) 359
BarI GAAGNNNNNNTAC 2 cut(s) 620, 652
BccI CCATC 2 cut(s) 853, 1021
BciVI GTATCC 2 cut(s) 582, 1045
BcoDI GTCTC 6 cut(s) 389, 412, 459, 750, 893, 929
BfaI CTAG 3 cut(s) 576, 644, 879
BfrI CTTAAG 2 cut(s) 449, 1073
BfuAI ACCTGC 1 cut(s) 347
BfuI GTATCC 2 cut(s) 582, 1045
BlnI CCTAGG 1 cut(s) 643
Bme18I GGWCC 1 cut(s) 57
BmeT110I CYCGRG 1 cut(s) 53
BmgT120I GGNCC 2 cut(s) 57, 518
BmiI GGNNCC 3 cut(s) 58, 361, 552
BmsI GCATC 4 cut(s) 77, 359, 818, 855
BplI GAGNNNNNCTC 2 cut(s) 539, 571
BsaBI GATNNNNATC 1 cut(s) 110
BsaHI GRCGYC 1 cut(s) 562
BsaJI CCNNGG 1 cut(s) 643
BsaWI WCCGGW 1 cut(s) 335
BsaXI ACNNNNNCTCC 2 cut(s) 977, 1007
Bsc4I CCNNNNNNNGG 2 cut(s) 683, 859
Bse118I RCCGGY 1 cut(s) 362
Bse1I ACTGG 1 cut(s) 381
Bse21I CCTNAGG 1 cut(s) 728
Bse3DI GCAATG 1 cut(s) 438
Bse8I GATNNNNATC 1 cut(s) 110
BseDI CCNNGG 1 cut(s) 643
BseGI GGATG 3 cut(s) 692, 917, 1009
BseJI GATNNNNATC 1 cut(s) 110
BseLI CCNNNNNNNGG 2 cut(s) 683, 859
BseMI GCAATG 1 cut(s) 438
BseMII CTCAG 1 cut(s) 812
BseNI ACTGG 1 cut(s) 381
BseYI CCCAGC 1 cut(s) 100
BsgI GTGCAG 1 cut(s) 534
BshFI GGCC 2 cut(s) 80, 520
BshNI GGYRCC 1 cut(s) 359
BsiHKCI CYCGRG 1 cut(s) 53
BsiSI CCGG 3 cut(s) 336, 363, 652
BslI CCNNNNNNNGG 2 cut(s) 683, 859
BsmAI GTCTC 6 cut(s) 389, 412, 459, 750, 893, 929
BsmBI CGTCTC 2 cut(s) 893, 929
BsnI GGCC 2 cut(s) 80, 520
BsoBI CYCGRG 1 cut(s) 53
Bsp143I GATC 3 cut(s) 199, 483, 828
BspACI CCGC 3 cut(s) 92, 677, 718
BspANI GGCC 2 cut(s) 80, 520
BspCNI CTCAG 1 cut(s) 811
BspLI GGNNCC 3 cut(s) 58, 361, 552
BspMI ACCTGC 1 cut(s) 347
BspPI GGATC 1 cut(s) 823
BspT107I GGYRCC 1 cut(s) 359
BspTI CTTAAG 2 cut(s) 449, 1073
BsrDI GCAATG 1 cut(s) 438
BsrFI RCCGGY 1 cut(s) 362
BsrI ACTGG 1 cut(s) 381
BssAI RCCGGY 1 cut(s) 362
BssECI CCNNGG 1 cut(s) 643
BssMI GATC 3 cut(s) 199, 483, 828
BssNI GRCGYC 1 cut(s) 562
BssT1I CCWWGG 1 cut(s) 643
Bst4CI ACNGT 2 cut(s) 130, 285
Bst6I CTCTTC 1 cut(s) 38
BstACI GRCGYC 1 cut(s) 562
BstAFI CTTAAG 2 cut(s) 449, 1073
BstDEI CTNAG 4 cut(s) 605, 728, 798, 1056
BstF5I GGATG 3 cut(s) 692, 917, 1009
BstKTI GATC 3 cut(s) 202, 486, 831
BstMAI GTCTC 6 cut(s) 389, 412, 459, 750, 893, 929
BstMBI GATC 3 cut(s) 199, 483, 828
BstMWI GCNNNNNNNGC 1 cut(s) 648
BstNSI RCATGY 2 cut(s) 185, 571
BstX2I RGATCY 1 cut(s) 828
BstYI RGATCY 1 cut(s) 828
Bsu36I CCTNAGG 1 cut(s) 728
BsuI GTATCC 2 cut(s) 582, 1045
BsuRI GGCC 2 cut(s) 80, 520
BtsCI GGATG 3 cut(s) 692, 917, 1009
BveI ACCTGC 1 cut(s) 347
Cfr10I RCCGGY 1 cut(s) 362
Cfr13I GGNCC 2 cut(s) 57, 518
CseI GACGC 1 cut(s) 551
CviAII CATG 6 cut(s) 182, 478, 558, 568, 946, 967
DdeI CTNAG 4 cut(s) 605, 728, 798, 1056
DpnI GATC 3 cut(s) 201, 485, 830
DpnII GATC 3 cut(s) 199, 483, 828
DraI TTTAAA 1 cut(s) 895
Eam1104I CTCTTC 1 cut(s) 38
EarI CTCTTC 1 cut(s) 38
Eco130I CCWWGG 1 cut(s) 643
Eco47I GGWCC 1 cut(s) 57
Eco57I CTGAAG 3 cut(s) 154, 767, 1038
Eco81I CCTNAGG 1 cut(s) 728
Eco88I CYCGRG 1 cut(s) 53
EcoO109I RGGNCCY 2 cut(s) 57, 518
EcoT14I CCWWGG 1 cut(s) 643
ErhI CCWWGG 1 cut(s) 643
Esp3I CGTCTC 2 cut(s) 893, 929
FaeI CATG 6 cut(s) 185, 481, 561, 571, 949, 970
FalI AAGNNNNNCTT 2 cut(s) 798, 830
FatI CATG 6 cut(s) 181, 477, 557, 567, 945, 966
FblI GTMKAC 1 cut(s) 132
FokI GGATG 3 cut(s) 699, 924, 996
FspBI CTAG 3 cut(s) 576, 644, 879
GsaI CCCAGC 1 cut(s) 104
HaeIII GGCC 2 cut(s) 80, 520
HapII CCGG 3 cut(s) 336, 363, 652
HgaI GACGC 1 cut(s) 551
Hin1I GRCGYC 1 cut(s) 562
Hin1II CATG 6 cut(s) 185, 481, 561, 571, 949, 970
HincII GTYRAC 1 cut(s) 133
HindII GTYRAC 1 cut(s) 133
HindIII AAGCTT 1 cut(s) 22
HpaII CCGG 3 cut(s) 336, 363, 652
HphI GGTGA 3 cut(s) 131, 207, 307
Hpy166II GTNNAC 4 cut(s) 6, 133, 674, 692
Hpy188III TCNNGA 5 cut(s) 388, 430, 535, 625, 1049
Hpy8I GTNNAC 4 cut(s) 6, 133, 674, 692
Hpy99I CGWCG 2 cut(s) 567, 907
HpyAV CCTTC 2 cut(s) 165, 622
HpyCH4III ACNGT 2 cut(s) 130, 285
HpyCH4IV ACGT 1 cut(s) 723
HpyCH4V TGCA 3 cut(s) 63, 74, 515
HpyF10VI GCNNNNNNNGC 1 cut(s) 648
HpyF3I CTNAG 4 cut(s) 605, 728, 798, 1056
HpySE526I ACGT 1 cut(s) 723
Hsp92I GRCGYC 1 cut(s) 562
Hsp92II CATG 6 cut(s) 185, 481, 561, 571, 949, 970
Kzo9I GATC 3 cut(s) 199, 483, 828
LmnI GCTCC 2 cut(s) 631, 873
LweI GCATC 4 cut(s) 77, 359, 818, 855
MaeI CTAG 3 cut(s) 576, 644, 879
MaeII ACGT 1 cut(s) 723
MaeIII GTNAC 3 cut(s) 331, 365, 434
MalI GATC 3 cut(s) 201, 485, 830
MboI GATC 3 cut(s) 199, 483, 828
MboII GAAGA 5 cut(s) 25, 160, 237, 493, 954
MflI RGATCY 1 cut(s) 828
MluCI AATT 9 cut(s) 148, 195, 243, 327, 538, 619, 657, 713, 1030
MlyI GAGTC 3 cut(s) 348, 752, 995
MmeI TCCRAC 2 cut(s) 276, 646
MnlI CCTC 6 cut(s) 303, 541, 565, 662, 807, 902
MroXI GAANNNNTTC 1 cut(s) 245
MseI TTAA 5 cut(s) 450, 615, 894, 1074, 1081
MspCI CTTAAG 2 cut(s) 449, 1073
MspI CCGG 3 cut(s) 336, 363, 652
MwoI GCNNNNNNNGC 1 cut(s) 648
NdeII GATC 3 cut(s) 199, 483, 828
NlaIII CATG 6 cut(s) 185, 481, 561, 571, 949, 970
NlaIV GGNNCC 3 cut(s) 58, 361, 552
NspI RCATGY 2 cut(s) 185, 571
PaqCI CACCTGC 1 cut(s) 347
PciI ACATGT 1 cut(s) 181
PcsI WCGNNNNNNNCGW 1 cut(s) 395
PdmI GAANNNNTTC 1 cut(s) 245
PfeI GAWTC 8 cut(s) 106, 122, 228, 400, 697, 976, 1006, 1060
PleI GAGTC 3 cut(s) 347, 752, 995
PpsI GAGTC 3 cut(s) 347, 752, 995
PpuMI RGGWCCY 1 cut(s) 57
PscI ACATGT 1 cut(s) 181
Psp5II RGGWCCY 1 cut(s) 57
PspFI CCCAGC 1 cut(s) 100
PspN4I GGNNCC 3 cut(s) 58, 361, 552
PspPI GGNCC 2 cut(s) 57, 518
PspPPI RGGWCCY 1 cut(s) 57
PsuI RGATCY 1 cut(s) 828
SalI GTCGAC 1 cut(s) 131
SaqAI TTAA 5 cut(s) 450, 615, 894, 1074, 1081
Sau3AI GATC 3 cut(s) 199, 483, 828
Sau96I GGNCC 2 cut(s) 57, 518
SchI GAGTC 3 cut(s) 348, 752, 995
SfaNI GCATC 4 cut(s) 77, 359, 818, 855
SinI GGWCC 1 cut(s) 57
SmlI CTYRAG 2 cut(s) 449, 1073
SmoI CTYRAG 2 cut(s) 449, 1073
Sse9I AATT 9 cut(s) 148, 195, 243, 327, 538, 619, 657, 713, 1030
SsiI CCGC 3 cut(s) 92, 677, 718
SspMI CTAG 3 cut(s) 576, 644, 879
StyI CCWWGG 1 cut(s) 643
TaaI ACNGT 2 cut(s) 130, 285
TaiI ACGT 1 cut(s) 726
TaqI TCGA 2 cut(s) 132, 1004
TasI AATT 9 cut(s) 148, 195, 243, 327, 538, 619, 657, 713, 1030
TfiI GAWTC 8 cut(s) 106, 122, 228, 400, 697, 976, 1006, 1060
Tru1I TTAA 5 cut(s) 450, 615, 894, 1074, 1081
Tru9I TTAA 5 cut(s) 450, 615, 894, 1074, 1081
TspDTI ATGAA 5 cut(s) 25, 494, 520, 934, 998
TspGWI ACGGA 3 cut(s) 207, 237, 903
Vha464I CTTAAG 2 cut(s) 449, 1073
VpaK11BI GGWCC 1 cut(s) 57
XapI RAATTY 2 cut(s) 243, 657
XceI RCATGY 2 cut(s) 185, 571
XmaJI CCTAGG 1 cut(s) 643
XmiI GTMKAC 1 cut(s) 132
XmnI GAANNNNTTC 1 cut(s) 245
XspI CTAG 3 cut(s) 576, 644, 879
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.