Rh5BG489800

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0001786
Physical Location & Seq
Reverse (-)
77954035 .. 77956324
2290 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG489800.1

Sequence Viewer

Length: 1140 bp
ATGGCACCCACAGGCACAACCCTCTTTCTAATTATACTAGTCCACATTTGGTTTTGCACCTTATTATTCATTTCCAACACATGCAAAGCCACAAGGCCTACACCAAAATTCCCAGCCATTCTAGTCTTTGGGGATTCAACAGTGGACACAGGCAACAACAATTACCTCAAAACATTGTTTAAGGGTAACCATTATCCATATGGCCAAGATTTTCCAGGTCATGTTACTACAGGCAGGTTTTCTAATGGAAAATTGGTTCCCGACTTCCTTGCCTCCATGTTAAACATCAAAGAAACTGTTCCTCCTTTTCTTGATCCGAGCCTCTCAGATAACAACCTTGTCACCGGTGTCAGCTTTGCTTCGGGTGGATCCGGATTCGACGATATTACAGGTAGTGTAGGTGGGATCATTCCATTTTCAAAGCAGGTTGAGTACTTCAAGAGATACATAGTGAGAGTCCAAGGTATTGTAGGGGAAAAGGAGGCAAAGAAGTTAATTAGTAGTGCAGTGGTTGTGATCAGTGCTGGGACTAATGACTTTGGTTTTAATTTCTATGACATCCCCACAAGAAGGTTAGAGTTCAATATTAGTGGCTACCAAGATTTTCTGCAGAATAAACTGCATATATTCATAGAGGAACTTTATGAGCTTGGATGCAGAAGAATGGCTATAGCTGGGCTTCCTCCAATAGGTTGCCTACCCATACAAATAACTGTAAAAATTGAAAGTCCACATGATAGAAGATGTATAGAGGAAGAAAATTTGGATGCACTTGTATACAATAAGAAGCTTGCAAGGCTCTTGCCCAAAATACAATCACTGCTGCCAAGGAGCAAAATTGTGTATGCAGACGTATATATGCCTTTTATTGACATGATCAATAATCCACTAAACTTTGGTTTTCTGGAAACAAAAATAGGCTGCTGTGGTACTGGGTTAGTGGAAACAGGACCCTTCTGTAATTCATTAACAGCAGTATGTGATAATGTCTTGGAGTACTTGTTTTGGGATAGCATACATCCAAGTCAAGCAGCATATCTCTACATTTCAAAATACCTGGAGAAGAAAATCATTCCCGAGCTTCTTGATTACGATAATCAATCACAGCCGTTAGTTACTCTATTTGATCAGACAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

379

Amino Acids

42.29

Weight (kDa)

5.91

Isoelectric Point (pI)

44.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 40 - 352 1.8e-33 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 225, 415
AccB1I GGYRCC 1 cut(s) 4
AccI GTMKAC 1 cut(s) 777
AccIII TCCGGA 1 cut(s) 371
AclWI GGATC 4 cut(s) 308, 363, 376, 413
AcoI YGGCCR 1 cut(s) 202
AcsI RAATTY 2 cut(s) 107, 760
AfaI GTAC 3 cut(s) 434, 931, 998
AfiI CCNNNNNNNGG 2 cut(s) 570, 689
AgeI ACCGGT 1 cut(s) 344
AgsI TTSAA 6 cut(s) 138, 420, 439, 583, 725, 1050
AhlI ACTAGT 1 cut(s) 37
AjnI CCWGG 2 cut(s) 214, 1056
AloI GAACNNNNNNTCC 2 cut(s) 240, 272
AluBI AGCT 5 cut(s) 354, 649, 674, 790, 1081
AluI AGCT 5 cut(s) 354, 649, 674, 790, 1081
AlwI GGATC 4 cut(s) 308, 363, 376, 413
Ama87I CYCGRG 1 cut(s) 1076
Aor13HI TCCGGA 1 cut(s) 371
AoxI GGCC 2 cut(s) 95, 202
ApeKI GCWGC 3 cut(s) 823, 921, 1031
ApoI RAATTY 2 cut(s) 107, 760
AsiGI ACCGGT 1 cut(s) 344
Asp700I GAANNNNTTC 1 cut(s) 297
AspS9I GGNCC 1 cut(s) 950
AsuHPI GGTGA 1 cut(s) 334
AvaI CYCGRG 1 cut(s) 1076
AvaII GGWCC 1 cut(s) 950
BalI TGGCCA 1 cut(s) 204
BamHI GGATCC 1 cut(s) 368
BanI GGYRCC 1 cut(s) 4
BbvI GCAGC 3 cut(s) 810, 908, 1043
BceAI ACGGC 1 cut(s) 1093
BcgI CGANNNNNNTGC 2 cut(s) 251, 285
BciT130I CCWGG 2 cut(s) 216, 1058
BclI TGATCA 3 cut(s) 516, 876, 1126
BcuI ACTAGT 1 cut(s) 37
BfaI CTAG 2 cut(s) 38, 122
BfmI CTRYAG 3 cut(s) 228, 608, 669
BfuAI ACCTGC 2 cut(s) 225, 415
BisI GCNGC 3 cut(s) 824, 922, 1032
BlsI GCNGC 3 cut(s) 825, 923, 1033
BmcAI AGTACT 2 cut(s) 434, 998
Bme1390I CCNGG 2 cut(s) 216, 1058
Bme18I GGWCC 1 cut(s) 950
BmeT110I CYCGRG 1 cut(s) 1076
BmgT120I GGNCC 1 cut(s) 950
BmiI GGNNCC 4 cut(s) 6, 258, 370, 952
BmrFI CCNGG 2 cut(s) 216, 1058
BmrI ACTGGG 1 cut(s) 942
BmsI GCATC 2 cut(s) 644, 757
BmuI ACTGGG 1 cut(s) 942
BpmI CTGGAG 1 cut(s) 1079
BsaJI CCNNGG 2 cut(s) 460, 827
BsaWI WCCGGW 2 cut(s) 344, 371
BsaXI ACNNNNNCTCC 4 cut(s) 286, 316, 986, 1016
Bsc4I CCNNNNNNNGG 2 cut(s) 570, 689
Bse118I RCCGGY 1 cut(s) 344
Bse1I ACTGG 1 cut(s) 937
BseAI TCCGGA 1 cut(s) 371
BseBI CCWGG 2 cut(s) 216, 1058
BseDI CCNNGG 2 cut(s) 460, 827
BseGI GGATG 4 cut(s) 558, 659, 772, 1018
BseLI CCNNNNNNNGG 2 cut(s) 570, 689
BseMII CTCAG 1 cut(s) 339
BseNI ACTGG 1 cut(s) 937
BseXI GCAGC 3 cut(s) 810, 908, 1043
BseYI CCCAGC 3 cut(s) 112, 524, 674
BsgI GTGCAG 1 cut(s) 525
BshFI GGCC 2 cut(s) 97, 204
BshNI GGYRCC 1 cut(s) 4
BshTI ACCGGT 1 cut(s) 344
BsiHKCI CYCGRG 1 cut(s) 1076
BsiSI CCGG 2 cut(s) 345, 372
BslFI GGGAC 1 cut(s) 541
BslI CCNNNNNNNGG 2 cut(s) 570, 689
BsmFI GGGAC 1 cut(s) 541
BsnI GGCC 2 cut(s) 97, 204
BsoBI CYCGRG 1 cut(s) 1076
Bsp13I TCCGGA 1 cut(s) 371
Bsp143I GATC 6 cut(s) 313, 368, 405, 516, 876, 1126
BspANI GGCC 2 cut(s) 97, 204
BspCNI CTCAG 1 cut(s) 338
BspEI TCCGGA 1 cut(s) 371
BspLI GGNNCC 4 cut(s) 6, 258, 370, 952
BspMAI CTGCAG 1 cut(s) 612
BspMI ACCTGC 2 cut(s) 225, 415
BspPI GGATC 4 cut(s) 308, 363, 376, 413
BspT107I GGYRCC 1 cut(s) 4
BsrFI RCCGGY 1 cut(s) 344
BsrI ACTGG 1 cut(s) 937
BssAI RCCGGY 1 cut(s) 344
BssECI CCNNGG 2 cut(s) 460, 827
BssMI GATC 6 cut(s) 313, 368, 405, 516, 876, 1126
BssNAI GTATAC 1 cut(s) 778
BssT1I CCWWGG 2 cut(s) 460, 827
Bst1107I GTATAC 1 cut(s) 778
Bst2UI CCWGG 2 cut(s) 216, 1058
Bst4CI ACNGT 3 cut(s) 142, 298, 715
BstC8I GCNNGC 1 cut(s) 792
BstDEI CTNAG 1 cut(s) 325
BstEII GGTNACC 1 cut(s) 185
BstENI CCTNNNNNAGG 1 cut(s) 687
BstF5I GGATG 4 cut(s) 558, 659, 772, 1018
BstKTI GATC 6 cut(s) 316, 371, 408, 519, 879, 1129
BstMBI GATC 6 cut(s) 313, 368, 405, 516, 876, 1126
BstMWI GCNNNNNNNGC 1 cut(s) 796
BstNI CCWGG 2 cut(s) 216, 1058
BstNSI RCATGY 1 cut(s) 84
BstPI GGTNACC 1 cut(s) 185
BstSCI CCNGG 2 cut(s) 214, 1056
BstSFI CTRYAG 3 cut(s) 228, 608, 669
BstV1I GCAGC 3 cut(s) 810, 908, 1043
BstX2I RGATCY 1 cut(s) 368
BstYI RGATCY 1 cut(s) 368
BstZ17I GTATAC 1 cut(s) 778
BsuRI GGCC 2 cut(s) 97, 204
BtsCI GGATG 4 cut(s) 558, 659, 772, 1018
BtsI GCAGTG 2 cut(s) 513, 818
BtsIMutI CAGTG 4 cut(s) 147, 513, 526, 818
BveI ACCTGC 2 cut(s) 225, 415
Cac8I GCNNGC 1 cut(s) 792
Cfr10I RCCGGY 1 cut(s) 344
Cfr13I GGNCC 1 cut(s) 950
Csp6I GTAC 3 cut(s) 433, 930, 997
CspAI ACCGGT 1 cut(s) 344
CspCI CAANNNNNGTGG 2 cut(s) 571, 606
CviAII CATG 5 cut(s) 81, 221, 277, 734, 874
CviQI GTAC 3 cut(s) 433, 930, 997
DdeI CTNAG 1 cut(s) 325
DpnI GATC 6 cut(s) 315, 370, 407, 518, 878, 1128
DpnII GATC 6 cut(s) 313, 368, 405, 516, 876, 1126
EaeI YGGCCR 1 cut(s) 202
Eco130I CCWWGG 2 cut(s) 460, 827
Eco147I AGGCCT 1 cut(s) 97
Eco47I GGWCC 1 cut(s) 950
Eco88I CYCGRG 1 cut(s) 1076
Eco91I GGTNACC 1 cut(s) 185
EcoNI CCTNNNNNAGG 1 cut(s) 687
EcoO109I RGGNCCY 1 cut(s) 950
EcoO65I GGTNACC 1 cut(s) 185
EcoRII CCWGG 2 cut(s) 214, 1056
EcoT14I CCWWGG 2 cut(s) 460, 827
ErhI CCWWGG 2 cut(s) 460, 827
FaeI CATG 5 cut(s) 84, 224, 280, 737, 877
FaqI GGGAC 1 cut(s) 541
FatI CATG 5 cut(s) 80, 220, 276, 733, 873
FauNDI CATATG 1 cut(s) 199
FbaI TGATCA 3 cut(s) 516, 876, 1126
FblI GTMKAC 1 cut(s) 777
Fnu4HI GCNGC 3 cut(s) 824, 922, 1032
FokI GGATG 4 cut(s) 545, 666, 779, 1005
Fsp4HI GCNGC 3 cut(s) 824, 922, 1032
FspBI CTAG 2 cut(s) 38, 122
GluI GCNGC 3 cut(s) 824, 922, 1032
GsaI CCCAGC 3 cut(s) 116, 528, 678
GsuI CTGGAG 1 cut(s) 1079
HaeIII GGCC 2 cut(s) 97, 204
HapII CCGG 2 cut(s) 345, 372
Hin1II CATG 5 cut(s) 84, 224, 280, 737, 877
HindIII AAGCTT 1 cut(s) 788
HinfI GANTC 3 cut(s) 134, 375, 456
HpaII CCGG 2 cut(s) 345, 372
HphI GGTGA 1 cut(s) 334
Hpy166II GTNNAC 4 cut(s) 43, 145, 731, 778
Hpy188I TCNGA 3 cut(s) 318, 328, 1131
Hpy188III TCNNGA 7 cut(s) 260, 311, 372, 439, 905, 1076, 1085
Hpy8I GTNNAC 4 cut(s) 43, 145, 731, 778
Hpy99I CGWCG 1 cut(s) 383
HpyAV CCTTC 2 cut(s) 564, 964
HpyCH4III ACNGT 3 cut(s) 142, 298, 715
HpyCH4IV ACGT 1 cut(s) 852
HpyCH4V TGCA 9 cut(s) 57, 84, 506, 610, 622, 657, 770, 794, 848
HpyF10VI GCNNNNNNNGC 1 cut(s) 796
HpyF3I CTNAG 1 cut(s) 325
HpySE526I ACGT 1 cut(s) 852
Hsp92II CATG 5 cut(s) 84, 224, 280, 737, 877
Kpn2I TCCGGA 1 cut(s) 371
Ksp22I TGATCA 3 cut(s) 516, 876, 1126
Kzo9I GATC 6 cut(s) 313, 368, 405, 516, 876, 1126
LmnI GCTCC 1 cut(s) 831
Lsp1109I GCAGC 3 cut(s) 810, 908, 1043
LweI GCATC 2 cut(s) 644, 757
MaeI CTAG 2 cut(s) 38, 122
MaeII ACGT 1 cut(s) 852
MaeIII GTNAC 4 cut(s) 185, 223, 340, 1114
MalI GATC 6 cut(s) 315, 370, 407, 518, 878, 1128
MboI GATC 6 cut(s) 313, 368, 405, 516, 876, 1126
MboII GAAGA 4 cut(s) 672, 753, 767, 1075
MflI RGATCY 1 cut(s) 368
MlsI TGGCCA 1 cut(s) 204
MluNI TGGCCA 1 cut(s) 204
MlyI GAGTC 1 cut(s) 465
MmeI TCCRAC 1 cut(s) 99
MnlI CCTC 9 cut(s) 32, 176, 283, 312, 332, 475, 628, 693, 745
Mox20I TGGCCA 1 cut(s) 204
MroI TCCGGA 1 cut(s) 371
MroXI GAANNNNTTC 1 cut(s) 297
MscI TGGCCA 1 cut(s) 204
MseI TTAA 5 cut(s) 180, 281, 494, 546, 968
Msp20I TGGCCA 1 cut(s) 204
MspI CCGG 2 cut(s) 345, 372
MspR9I CCNGG 2 cut(s) 216, 1058
MvaI CCWGG 2 cut(s) 216, 1058
MwoI GCNNNNNNNGC 1 cut(s) 796
NdeI CATATG 1 cut(s) 199
NdeII GATC 6 cut(s) 313, 368, 405, 516, 876, 1126
NlaIII CATG 5 cut(s) 84, 224, 280, 737, 877
NlaIV GGNNCC 4 cut(s) 6, 258, 370, 952
NmuCI GTSAC 1 cut(s) 340
NspI RCATGY 1 cut(s) 84
PceI AGGCCT 1 cut(s) 97
PdmI GAANNNNTTC 1 cut(s) 297
PfeI GAWTC 2 cut(s) 134, 375
PinAI ACCGGT 1 cut(s) 344
PkrI GCNGC 3 cut(s) 825, 923, 1033
PleI GAGTC 1 cut(s) 464
PpsI GAGTC 1 cut(s) 464
PpuMI RGGWCCY 1 cut(s) 950
Psp5II RGGWCCY 1 cut(s) 950
Psp6I CCWGG 2 cut(s) 214, 1056
PspEI GGTNACC 1 cut(s) 185
PspFI CCCAGC 3 cut(s) 112, 524, 674
PspGI CCWGG 2 cut(s) 214, 1056
PspN4I GGNNCC 4 cut(s) 6, 258, 370, 952
PspPI GGNCC 1 cut(s) 950
PspPPI RGGWCCY 1 cut(s) 950
PstI CTGCAG 1 cut(s) 612
PsuI RGATCY 1 cut(s) 368
RsaI GTAC 3 cut(s) 434, 931, 998
RsaNI GTAC 3 cut(s) 433, 930, 997
SaqAI TTAA 5 cut(s) 180, 281, 494, 546, 968
SatI GCNGC 3 cut(s) 824, 922, 1032
Sau3AI GATC 6 cut(s) 313, 368, 405, 516, 876, 1126
Sau96I GGNCC 1 cut(s) 950
ScaI AGTACT 2 cut(s) 434, 998
SchI GAGTC 1 cut(s) 465
ScrFI CCNGG 2 cut(s) 216, 1058
SfaNI GCATC 2 cut(s) 644, 757
SfcI CTRYAG 3 cut(s) 228, 608, 669
SgrAI CRCCGGYG 1 cut(s) 344
SinI GGWCC 1 cut(s) 950
SpeI ACTAGT 1 cut(s) 37
SseBI AGGCCT 1 cut(s) 97
SspI AATATT 1 cut(s) 586
SspMI CTAG 2 cut(s) 38, 122
StuI AGGCCT 1 cut(s) 97
StyD4I CCNGG 2 cut(s) 214, 1056
StyI CCWWGG 2 cut(s) 460, 827
TaaI ACNGT 3 cut(s) 142, 298, 715
TaiI ACGT 1 cut(s) 855
TaqI TCGA 1 cut(s) 378
TatI WGTACW 2 cut(s) 432, 996
TfiI GAWTC 2 cut(s) 134, 375
Tru1I TTAA 5 cut(s) 180, 281, 494, 546, 968
Tru9I TTAA 5 cut(s) 180, 281, 494, 546, 968
TscAI CASTG 4 cut(s) 147, 513, 526, 825
TseFI GTSAC 1 cut(s) 340
TseI GCWGC 3 cut(s) 823, 921, 1031
Tsp45I GTSAC 1 cut(s) 340
TspDTI ATGAA 3 cut(s) 58, 619, 954
TspRI CASTG 4 cut(s) 147, 513, 526, 825
VpaK11BI GGWCC 1 cut(s) 950
XagI CCTNNNNNAGG 1 cut(s) 687
XapI RAATTY 2 cut(s) 107, 760
XceI RCATGY 1 cut(s) 84
XcmI CCANNNNNNNNNTGG 1 cut(s) 197
XmiI GTMKAC 1 cut(s) 777
XmnI GAANNNNTTC 1 cut(s) 297
XspI CTAG 2 cut(s) 38, 122
ZrmI AGTACT 2 cut(s) 434, 998
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.