RchiOBHm_Chr5g0071691

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001786
Physical Location & Seq
Reverse (-)
77465796 .. 77470668
4873 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34673

Sequence Viewer

Length: 1203 bp
ATGTCCCTTACGGACATGTTCCTGCTGGTCCGGCCTCGCCGGCAGCTTCAGAACAAATTAACAATGGCACCCACAGGCACAACCCTCTTTCTAATTATACTAGTCCACATTTGGTTTTGCACCTTATTATTCATTTCCAACACATGCAAAGCCACAAGGCCTACACCAAAATTCCCAGCCATTCTAGTCTTTGGGGATTCAACAGTGGACACAGGCAACAACAATTACCTCAAAACATTGTTTAAGGGTAACCATTATCCATATGGCCAAGATTTTCCAGGTCATGTTACTACAGGCAGGTTTTCTAATGGAAAATTGGTTCCCGACTTCCTTGCCTCCATGTTAAACATCAAAGAAACTGTTCCTCCTTTTCTTGATCCGAGCCTCTCGGATACCAACCTTGTCACCGGTGTCAGCTTTGCTTCGGGTGGATCTGGATTCGACGATATTACAGGTAGTGTAGGTGGGATCATTCCATTTTCAAAGCAGGTTGAGTACTTCAAGAGATACATAGTGAGAGTCCAAGGTATTGTAGGGGAAAAGGAGGCAAAGAAGTTAATTAGTAGTGCAGTGGTTGTGATCAGTGCTGGGACTAATGACTTTGGTTTTAATTTCTATGACATCCCCACAAGAAGGTTAGAGTTCAATATTAGTGGCTACCAAGATTTTCTGCAGAATAAACTGCATATATTCATAGAGGAACTTTATGAGCTTGGATGCAGAAGAATGGCTATAGCTGGGCTTCCTCCAATAGGTTGCCTACCCATACAAATAACTGTAAAAGTCGAAAGTCCACATGATAGAAGATGTATAGAGGAAGAAAATTTGGATGCACTTGTATACAATAAGAAGCTTGCAAGCCTCTTGCCCAAAATACAATCACTGCTGCCAAGGAGCAAAATTGTGTATGCAGACGTGTATATGCCTTTAATTGACATGATCAATAATCCACTAAACTTTGGTTTTCTGGAAACAAAAAGAGGCTGCTGTGGTACTGGGTTAGTGGAAACAGGACCCTTCTGTAATTCATTAACAGCAGTATGTGATAATGTCTTGGAGTACTTGTTTTGGGATAGCATACATCCAAGTCAAGCAGCATATCTCTACATTTCAAAATACTTGGAGAAGAAAAGCATTCCCGAGCTTCTTGATTACGATAATCAATCACAGCCGTTAGTTACTCTATTTGATCAGACAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

400

Amino Acids

44.72

Weight (kDa)

6.6

Isoelectric Point (pI)

45.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 61 - 373 3.1e-33 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 288, 478
AccB1I GGYRCC 1 cut(s) 67
AccI GTMKAC 1 cut(s) 840
AclWI GGATC 3 cut(s) 371, 439, 476
AcoI YGGCCR 1 cut(s) 265
AcsI RAATTY 2 cut(s) 170, 823
AcuI CTGAAG 1 cut(s) 32
AfaI GTAC 3 cut(s) 497, 994, 1061
AfiI CCNNNNNNNGG 2 cut(s) 633, 752
AflIII ACRYGT 2 cut(s) 15, 915
AgeI ACCGGT 1 cut(s) 407
AgsI TTSAA 5 cut(s) 201, 483, 502, 646, 1113
AhlI ACTAGT 1 cut(s) 100
AjiI CACGTC 1 cut(s) 916
AjnI CCWGG 1 cut(s) 277
AloI GAACNNNNNNTCC 2 cut(s) 303, 335
AluBI AGCT 6 cut(s) 46, 417, 712, 737, 853, 1144
AluI AGCT 6 cut(s) 46, 417, 712, 737, 853, 1144
AlwI GGATC 3 cut(s) 371, 439, 476
Ama87I CYCGRG 1 cut(s) 1139
AoxI GGCC 3 cut(s) 32, 158, 265
ApeKI GCWGC 4 cut(s) 43, 886, 984, 1094
ApoI RAATTY 2 cut(s) 170, 823
AsiGI ACCGGT 1 cut(s) 407
Asp700I GAANNNNTTC 1 cut(s) 360
AspS9I GGNCC 2 cut(s) 28, 1013
AsuHPI GGTGA 1 cut(s) 397
AvaI CYCGRG 1 cut(s) 1139
AvaII GGWCC 2 cut(s) 28, 1013
BalI TGGCCA 1 cut(s) 267
BanI GGYRCC 1 cut(s) 67
BbvI GCAGC 4 cut(s) 55, 873, 971, 1106
BceAI ACGGC 1 cut(s) 1156
BcgI CGANNNNNNTGC 2 cut(s) 314, 348
BciT130I CCWGG 1 cut(s) 279
BciVI GTATCC 1 cut(s) 385
BclI TGATCA 3 cut(s) 579, 939, 1189
BcuI ACTAGT 1 cut(s) 100
BfaI CTAG 2 cut(s) 101, 185
BfmI CTRYAG 3 cut(s) 291, 671, 732
BfuAI ACCTGC 2 cut(s) 288, 478
BfuI GTATCC 1 cut(s) 385
BglI GCCNNNNNGGC 1 cut(s) 40
BisI GCNGC 4 cut(s) 44, 887, 985, 1095
BlsI GCNGC 4 cut(s) 45, 888, 986, 1096
BmcAI AGTACT 2 cut(s) 497, 1061
Bme1390I CCNGG 1 cut(s) 279
Bme18I GGWCC 2 cut(s) 28, 1013
BmeT110I CYCGRG 1 cut(s) 1139
BmgBI CACGTC 1 cut(s) 916
BmgT120I GGNCC 2 cut(s) 28, 1013
BmiI GGNNCC 3 cut(s) 69, 321, 1015
BmrFI CCNGG 1 cut(s) 279
BmrI ACTGGG 1 cut(s) 1005
BmsI GCATC 2 cut(s) 707, 820
BmuI ACTGGG 1 cut(s) 1005
BsaJI CCNNGG 2 cut(s) 523, 890
BsaWI WCCGGW 1 cut(s) 407
BsaXI ACNNNNNCTCC 4 cut(s) 349, 379, 1049, 1079
Bsc4I CCNNNNNNNGG 2 cut(s) 633, 752
Bse118I RCCGGY 2 cut(s) 39, 407
Bse1I ACTGG 1 cut(s) 1000
BseBI CCWGG 1 cut(s) 279
BseDI CCNNGG 2 cut(s) 523, 890
BseGI GGATG 4 cut(s) 621, 722, 835, 1081
BseLI CCNNNNNNNGG 2 cut(s) 633, 752
BseNI ACTGG 1 cut(s) 1000
BseXI GCAGC 4 cut(s) 55, 873, 971, 1106
BseYI CCCAGC 3 cut(s) 175, 587, 737
BsgI GTGCAG 1 cut(s) 588
BshFI GGCC 3 cut(s) 34, 160, 267
BshNI GGYRCC 1 cut(s) 67
BshTI ACCGGT 1 cut(s) 407
BsiHKCI CYCGRG 1 cut(s) 1139
BsiSI CCGG 3 cut(s) 31, 40, 408
BslFI GGGAC 1 cut(s) 604
BslI CCNNNNNNNGG 2 cut(s) 633, 752
BsmFI GGGAC 1 cut(s) 604
BsmI GAATGC 1 cut(s) 1134
BsnI GGCC 3 cut(s) 34, 160, 267
BsoBI CYCGRG 1 cut(s) 1139
Bsp143I GATC 6 cut(s) 376, 431, 468, 579, 939, 1189
BspANI GGCC 3 cut(s) 34, 160, 267
BspLI GGNNCC 3 cut(s) 69, 321, 1015
BspMAI CTGCAG 1 cut(s) 675
BspMI ACCTGC 2 cut(s) 288, 478
BspPI GGATC 3 cut(s) 371, 439, 476
BspT107I GGYRCC 1 cut(s) 67
BsrFI RCCGGY 2 cut(s) 39, 407
BsrI ACTGG 1 cut(s) 1000
BssAI RCCGGY 2 cut(s) 39, 407
BssECI CCNNGG 2 cut(s) 523, 890
BssMI GATC 6 cut(s) 376, 431, 468, 579, 939, 1189
BssNAI GTATAC 1 cut(s) 841
BssT1I CCWWGG 2 cut(s) 523, 890
Bst1107I GTATAC 1 cut(s) 841
Bst2UI CCWGG 1 cut(s) 279
Bst4CI ACNGT 3 cut(s) 205, 361, 778
BstC8I GCNNGC 3 cut(s) 41, 855, 859
BstEII GGTNACC 1 cut(s) 248
BstENI CCTNNNNNAGG 1 cut(s) 750
BstF5I GGATG 4 cut(s) 621, 722, 835, 1081
BstKTI GATC 6 cut(s) 379, 434, 471, 582, 942, 1192
BstMBI GATC 6 cut(s) 376, 431, 468, 579, 939, 1189
BstMWI GCNNNNNNNGC 2 cut(s) 31, 40
BstNI CCWGG 1 cut(s) 279
BstNSI RCATGY 2 cut(s) 19, 147
BstPI GGTNACC 1 cut(s) 248
BstSCI CCNGG 1 cut(s) 277
BstSFI CTRYAG 3 cut(s) 291, 671, 732
BstV1I GCAGC 4 cut(s) 55, 873, 971, 1106
BstX2I RGATCY 1 cut(s) 431
BstYI RGATCY 1 cut(s) 431
BstZ17I GTATAC 1 cut(s) 841
BsuI GTATCC 1 cut(s) 385
BsuRI GGCC 3 cut(s) 34, 160, 267
BtrI CACGTC 1 cut(s) 916
BtsCI GGATG 4 cut(s) 621, 722, 835, 1081
BtsI GCAGTG 2 cut(s) 576, 881
BtsIMutI CAGTG 4 cut(s) 210, 576, 589, 881
BveI ACCTGC 2 cut(s) 288, 478
Cac8I GCNNGC 3 cut(s) 41, 855, 859
Cfr10I RCCGGY 2 cut(s) 39, 407
Cfr13I GGNCC 2 cut(s) 28, 1013
Csp6I GTAC 3 cut(s) 496, 993, 1060
CspAI ACCGGT 1 cut(s) 407
CspCI CAANNNNNGTGG 2 cut(s) 634, 669
CviAII CATG 6 cut(s) 16, 144, 284, 340, 797, 937
CviQI GTAC 3 cut(s) 496, 993, 1060
DpnI GATC 6 cut(s) 378, 433, 470, 581, 941, 1191
DpnII GATC 6 cut(s) 376, 431, 468, 579, 939, 1189
EaeI YGGCCR 1 cut(s) 265
Eco130I CCWWGG 2 cut(s) 523, 890
Eco147I AGGCCT 1 cut(s) 160
Eco47I GGWCC 2 cut(s) 28, 1013
Eco57I CTGAAG 1 cut(s) 32
Eco88I CYCGRG 1 cut(s) 1139
Eco91I GGTNACC 1 cut(s) 248
EcoNI CCTNNNNNAGG 1 cut(s) 750
EcoO109I RGGNCCY 1 cut(s) 1013
EcoO65I GGTNACC 1 cut(s) 248
EcoRII CCWGG 1 cut(s) 277
EcoT14I CCWWGG 2 cut(s) 523, 890
ErhI CCWWGG 2 cut(s) 523, 890
FaeI CATG 6 cut(s) 19, 147, 287, 343, 800, 940
FaqI GGGAC 1 cut(s) 604
FatI CATG 6 cut(s) 15, 143, 283, 339, 796, 936
FauNDI CATATG 1 cut(s) 262
FbaI TGATCA 3 cut(s) 579, 939, 1189
FblI GTMKAC 1 cut(s) 840
Fnu4HI GCNGC 4 cut(s) 44, 887, 985, 1095
FokI GGATG 4 cut(s) 608, 729, 842, 1068
Fsp4HI GCNGC 4 cut(s) 44, 887, 985, 1095
FspBI CTAG 2 cut(s) 101, 185
GluI GCNGC 4 cut(s) 44, 887, 985, 1095
GsaI CCCAGC 3 cut(s) 179, 591, 741
HaeIII GGCC 3 cut(s) 34, 160, 267
HapII CCGG 3 cut(s) 31, 40, 408
Hin1II CATG 6 cut(s) 19, 147, 287, 343, 800, 940
HindIII AAGCTT 1 cut(s) 851
HinfI GANTC 3 cut(s) 197, 438, 519
HpaII CCGG 3 cut(s) 31, 40, 408
HphI GGTGA 1 cut(s) 397
Hpy166II GTNNAC 4 cut(s) 106, 208, 794, 841
Hpy188I TCNGA 4 cut(s) 51, 381, 391, 1194
Hpy188III TCNNGA 7 cut(s) 323, 374, 435, 502, 968, 1139, 1148
Hpy8I GTNNAC 4 cut(s) 106, 208, 794, 841
Hpy99I CGWCG 1 cut(s) 446
HpyAV CCTTC 2 cut(s) 627, 1027
HpyCH4III ACNGT 3 cut(s) 205, 361, 778
HpyCH4IV ACGT 1 cut(s) 915
HpyCH4V TGCA 9 cut(s) 120, 147, 569, 673, 685, 720, 833, 857, 911
HpyF10VI GCNNNNNNNGC 2 cut(s) 31, 40
HpySE526I ACGT 1 cut(s) 915
Hsp92II CATG 6 cut(s) 19, 147, 287, 343, 800, 940
KroI GCCGGC 1 cut(s) 39
KroNI GCCGGC 1 cut(s) 41
Ksp22I TGATCA 3 cut(s) 579, 939, 1189
Kzo9I GATC 6 cut(s) 376, 431, 468, 579, 939, 1189
LmnI GCTCC 1 cut(s) 894
Lsp1109I GCAGC 4 cut(s) 55, 873, 971, 1106
LweI GCATC 2 cut(s) 707, 820
MaeI CTAG 2 cut(s) 101, 185
MaeII ACGT 1 cut(s) 915
MaeIII GTNAC 4 cut(s) 248, 286, 403, 1177
MalI GATC 6 cut(s) 378, 433, 470, 581, 941, 1191
MboI GATC 6 cut(s) 376, 431, 468, 579, 939, 1189
MboII GAAGA 4 cut(s) 735, 816, 830, 1138
MflI RGATCY 1 cut(s) 431
MlsI TGGCCA 1 cut(s) 267
MluNI TGGCCA 1 cut(s) 267
MlyI GAGTC 1 cut(s) 528
MmeI TCCRAC 1 cut(s) 162
Mox20I TGGCCA 1 cut(s) 267
MroNI GCCGGC 1 cut(s) 39
MroXI GAANNNNTTC 1 cut(s) 360
MscI TGGCCA 1 cut(s) 267
MseI TTAA 7 cut(s) 59, 243, 344, 557, 609, 929, 1031
Msp20I TGGCCA 1 cut(s) 267
MspI CCGG 3 cut(s) 31, 40, 408
MspR9I CCNGG 1 cut(s) 279
Mva1269I GAATGC 1 cut(s) 1134
MvaI CCWGG 1 cut(s) 279
MwoI GCNNNNNNNGC 2 cut(s) 31, 40
NaeI GCCGGC 1 cut(s) 41
NdeI CATATG 1 cut(s) 262
NdeII GATC 6 cut(s) 376, 431, 468, 579, 939, 1189
NgoMIV GCCGGC 1 cut(s) 39
NlaIII CATG 6 cut(s) 19, 147, 287, 343, 800, 940
NlaIV GGNNCC 3 cut(s) 69, 321, 1015
NmuCI GTSAC 1 cut(s) 403
NspI RCATGY 2 cut(s) 19, 147
PceI AGGCCT 1 cut(s) 160
PciI ACATGT 1 cut(s) 15
PctI GAATGC 1 cut(s) 1134
PdiI GCCGGC 1 cut(s) 41
PdmI GAANNNNTTC 1 cut(s) 360
PfeI GAWTC 2 cut(s) 197, 438
PinAI ACCGGT 1 cut(s) 407
PkrI GCNGC 4 cut(s) 45, 888, 986, 1096
PleI GAGTC 1 cut(s) 527
PpsI GAGTC 1 cut(s) 527
PpuMI RGGWCCY 1 cut(s) 1013
PscI ACATGT 1 cut(s) 15
Psp5II RGGWCCY 1 cut(s) 1013
Psp6I CCWGG 1 cut(s) 277
PspEI GGTNACC 1 cut(s) 248
PspFI CCCAGC 3 cut(s) 175, 587, 737
PspGI CCWGG 1 cut(s) 277
PspN4I GGNNCC 3 cut(s) 69, 321, 1015
PspPI GGNCC 2 cut(s) 28, 1013
PspPPI RGGWCCY 1 cut(s) 1013
PstI CTGCAG 1 cut(s) 675
PsuI RGATCY 1 cut(s) 431
RsaI GTAC 3 cut(s) 497, 994, 1061
RsaNI GTAC 3 cut(s) 496, 993, 1060
SaqAI TTAA 7 cut(s) 59, 243, 344, 557, 609, 929, 1031
SatI GCNGC 4 cut(s) 44, 887, 985, 1095
Sau3AI GATC 6 cut(s) 376, 431, 468, 579, 939, 1189
Sau96I GGNCC 2 cut(s) 28, 1013
ScaI AGTACT 2 cut(s) 497, 1061
SchI GAGTC 1 cut(s) 528
ScrFI CCNGG 1 cut(s) 279
SfaNI GCATC 2 cut(s) 707, 820
SfcI CTRYAG 3 cut(s) 291, 671, 732
SgrAI CRCCGGYG 1 cut(s) 407
SinI GGWCC 2 cut(s) 28, 1013
SpeI ACTAGT 1 cut(s) 100
SseBI AGGCCT 1 cut(s) 160
SspI AATATT 1 cut(s) 649
SspMI CTAG 2 cut(s) 101, 185
StuI AGGCCT 1 cut(s) 160
StyD4I CCNGG 1 cut(s) 277
StyI CCWWGG 2 cut(s) 523, 890
TaaI ACNGT 3 cut(s) 205, 361, 778
TaiI ACGT 1 cut(s) 918
TaqI TCGA 2 cut(s) 441, 786
TatI WGTACW 2 cut(s) 495, 1059
TfiI GAWTC 2 cut(s) 197, 438
Tru1I TTAA 7 cut(s) 59, 243, 344, 557, 609, 929, 1031
Tru9I TTAA 7 cut(s) 59, 243, 344, 557, 609, 929, 1031
TscAI CASTG 4 cut(s) 210, 576, 589, 888
TseFI GTSAC 1 cut(s) 403
TseI GCWGC 4 cut(s) 43, 886, 984, 1094
Tsp45I GTSAC 1 cut(s) 403
TspDTI ATGAA 3 cut(s) 121, 682, 1017
TspGWI ACGGA 1 cut(s) 26
TspRI CASTG 4 cut(s) 210, 576, 589, 888
VpaK11BI GGWCC 2 cut(s) 28, 1013
XagI CCTNNNNNAGG 1 cut(s) 750
XapI RAATTY 2 cut(s) 170, 823
XceI RCATGY 2 cut(s) 19, 147
XcmI CCANNNNNNNNNTGG 1 cut(s) 260
XmiI GTMKAC 1 cut(s) 840
XmnI GAANNNNTTC 1 cut(s) 360
XspI CTAG 2 cut(s) 101, 185
ZrmI AGTACT 2 cut(s) 497, 1061
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.