Rroxscaffold_1G00009480

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0001786
Physical Location & Seq
Forward (+)
12005307 .. 12008350
3044 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00009480.1

Sequence Viewer

Length: 1125 bp
ATGTTTTGCGAGCTAGACCGGCGTGATTCTAGATCAGGTCTTAACGATGTGAGTTGTGGCAGCATGAGGCCCGGCGGTTGGAGTCGAAGTGGCTTGAGGTCGTGGCAGCAAGGATTGTGGGGGTACAAGTCGTGGCAGAACTGGTCGAGCAGACGGATGAGCTGGTTTTGCTGTGTACGACATAGTGTCTTGGAGATGCTAGGTCATGTTACTACAGGCAGGTTTTCTAATGGAAAATTGGTTCCCGACTTCCTTGCCTCCATGTTAAACATCAAAGAAACTGTTCCTCCTTTTCTTGATCCGAGCCTCTCGGACAACAACCTTGTCACCGGTGTCAGCTTTGCTTCGGGTGGATCCGGATTCGACGATATTACAGGCAGTATAGGTGGGATCATTCCATTTTCAAAGCAGGTTGAGTACTTCAAGAGATACATAGTGAGAGTCCAAGGTATTGTAGGGGAAAAGGAGGCAAAGAAGTTAATTAGTAGTGCAGTGGTTGTGATCAGTGCTGGGACTAATGACTTTGGTTTTAATTTCTATGACATCCCCACAAGAAGGTTAGAGTTCAATATTAGTGGCTACCAAGATTTTCTGCAGAATAAACTGCATATATTCATAGAGGAACTTTATAAGCTTGGATGCAGAAGAATGGCTATAGCTGGGCTTCCTCCAATAGGTTGCCTACCCATACAAATAACTGTGAAATTTGAAAGTCCACATGATAGAAGATGTATAGAGGAAGAAAATTTGGGTGCACTTGTATACAATAAGAAGCTTGCAAGGCTCTTGCCCGAAATACAATCACTGCTGCCAAGGAGCAAAATTGTGTATGCAGACGTGTATATGCCTTTAATTGACATGATCAATAATCCACTAAACTTTGGTTTTCTGGAAACAAAAAGAGGCTGCTGTGGTACTGGGTTAGTGGAAGCAGGACCCTTCTGTAATTCGTTAACAGCAGTATGTGATAATGTCTTGGAGTACTTGTTTTGGGATAGCATACATCCAAGTCAAGCAGCATATCTCTACATTTCAAAATACCTGGAGAAGAAAATCATTCCCGAGCTTCTTGATTACGATAATCAATCACAGCCGTTAGTTACTCTATTTGATCAGACAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

374

Amino Acids

42.11

Weight (kDa)

7.48

Isoelectric Point (pI)

53.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 68 - 347 3.5e-28 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 630
Acc36I ACCTGC 2 cut(s) 210, 400
AccI GTMKAC 1 cut(s) 762
AccIII TCCGGA 1 cut(s) 356
AciI CCGC 1 cut(s) 75
AclWI GGATC 4 cut(s) 293, 348, 361, 398
AcsI RAATTY 2 cut(s) 704, 745
AfaI GTAC 5 cut(s) 125, 177, 419, 916, 983
AfiI CCNNNNNNNGG 3 cut(s) 78, 555, 674
AflIII ACRYGT 1 cut(s) 837
AgeI ACCGGT 1 cut(s) 329
AgsI TTSAA 5 cut(s) 405, 424, 568, 710, 1035
AhdI GACNNNNNGTC 1 cut(s) 185
AjiI CACGTC 1 cut(s) 838
AjnI CCWGG 1 cut(s) 1041
AloI GAACNNNNNNTCC 2 cut(s) 225, 257
AluBI AGCT 7 cut(s) 13, 162, 339, 634, 659, 775, 1066
AluI AGCT 7 cut(s) 13, 162, 339, 634, 659, 775, 1066
Alw21I GWGCWC 1 cut(s) 757
Alw44I GTGCAC 1 cut(s) 753
AlwI GGATC 4 cut(s) 293, 348, 361, 398
Ama87I CYCGRG 1 cut(s) 1061
Aor13HI TCCGGA 1 cut(s) 356
AoxI GGCC 1 cut(s) 68
ApaLI GTGCAC 1 cut(s) 753
ApeKI GCWGC 5 cut(s) 60, 106, 808, 906, 1016
ApoI RAATTY 2 cut(s) 704, 745
AsiGI ACCGGT 1 cut(s) 329
Asp700I GAANNNNTTC 1 cut(s) 282
AspS9I GGNCC 2 cut(s) 69, 935
AsuC2I CCSGG 1 cut(s) 72
AsuHPI GGTGA 1 cut(s) 319
AvaI CYCGRG 1 cut(s) 1061
AvaII GGWCC 1 cut(s) 935
BaeGI GKGCMC 1 cut(s) 757
BamHI GGATCC 1 cut(s) 353
Bbv12I GWGCWC 1 cut(s) 757
BbvI GCAGC 5 cut(s) 72, 118, 795, 893, 1028
BceAI ACGGC 1 cut(s) 1078
BcgI CGANNNNNNTGC 2 cut(s) 236, 270
BciT130I CCWGG 1 cut(s) 1043
BclI TGATCA 3 cut(s) 501, 861, 1111
BcnI CCSGG 1 cut(s) 72
BfaI CTAG 3 cut(s) 14, 30, 200
BfmI CTRYAG 3 cut(s) 213, 593, 654
BfuAI ACCTGC 2 cut(s) 210, 400
BisI GCNGC 5 cut(s) 61, 107, 809, 907, 1017
BlsI GCNGC 5 cut(s) 62, 108, 810, 908, 1018
BmcAI AGTACT 2 cut(s) 419, 983
Bme1390I CCNGG 2 cut(s) 72, 1043
Bme18I GGWCC 1 cut(s) 935
BmeRI GACNNNNNGTC 1 cut(s) 185
BmeT110I CYCGRG 1 cut(s) 1061
BmgBI CACGTC 1 cut(s) 838
BmgT120I GGNCC 2 cut(s) 69, 935
BmiI GGNNCC 3 cut(s) 243, 355, 937
BmrFI CCNGG 2 cut(s) 72, 1043
BmrI ACTGGG 1 cut(s) 927
BmsI GCATC 2 cut(s) 186, 629
BmuI ACTGGG 1 cut(s) 927
BpmI CTGGAG 1 cut(s) 1064
BpuEI CTTGAG 1 cut(s) 115
BpuMI CCSGG 1 cut(s) 72
BsaJI CCNNGG 2 cut(s) 445, 812
BsaWI WCCGGW 2 cut(s) 329, 356
BsaXI ACNNNNNCTCC 6 cut(s) 73, 103, 271, 301, 971, 1001
Bsc4I CCNNNNNNNGG 3 cut(s) 78, 555, 674
Bse118I RCCGGY 2 cut(s) 18, 329
Bse1I ACTGG 2 cut(s) 146, 922
BseAI TCCGGA 1 cut(s) 356
BseBI CCWGG 1 cut(s) 1043
BseDI CCNNGG 2 cut(s) 445, 812
BseGI GGATG 4 cut(s) 162, 543, 644, 1003
BseLI CCNNNNNNNGG 3 cut(s) 78, 555, 674
BseNI ACTGG 2 cut(s) 146, 922
BseSI GKGCMC 1 cut(s) 757
BseXI GCAGC 5 cut(s) 72, 118, 795, 893, 1028
BseYI CCCAGC 2 cut(s) 509, 659
BsgI GTGCAG 1 cut(s) 510
BshFI GGCC 1 cut(s) 70
BshTI ACCGGT 1 cut(s) 329
BsiHKAI GWGCWC 1 cut(s) 757
BsiHKCI CYCGRG 1 cut(s) 1061
BsiSI CCGG 4 cut(s) 19, 72, 330, 357
BslFI GGGAC 1 cut(s) 526
BslI CCNNNNNNNGG 3 cut(s) 78, 555, 674
BsmFI GGGAC 1 cut(s) 526
BsnI GGCC 1 cut(s) 70
BsoBI CYCGRG 1 cut(s) 1061
Bsp1286I GDGCHC 1 cut(s) 757
Bsp13I TCCGGA 1 cut(s) 356
Bsp143I GATC 7 cut(s) 32, 298, 353, 390, 501, 861, 1111
BspACI CCGC 1 cut(s) 75
BspANI GGCC 1 cut(s) 70
BspEI TCCGGA 1 cut(s) 356
BspLI GGNNCC 3 cut(s) 243, 355, 937
BspMAI CTGCAG 1 cut(s) 597
BspMI ACCTGC 2 cut(s) 210, 400
BspPI GGATC 4 cut(s) 293, 348, 361, 398
BsrFI RCCGGY 2 cut(s) 18, 329
BsrI ACTGG 2 cut(s) 146, 922
BssAI RCCGGY 2 cut(s) 18, 329
BssECI CCNNGG 2 cut(s) 445, 812
BssMI GATC 7 cut(s) 32, 298, 353, 390, 501, 861, 1111
BssNAI GTATAC 1 cut(s) 763
BssT1I CCWWGG 2 cut(s) 445, 812
Bst1107I GTATAC 1 cut(s) 763
Bst2UI CCWGG 1 cut(s) 1043
Bst4CI ACNGT 2 cut(s) 283, 700
BstC8I GCNNGC 2 cut(s) 11, 777
BstENI CCTNNNNNAGG 1 cut(s) 672
BstF5I GGATG 4 cut(s) 162, 543, 644, 1003
BstKTI GATC 7 cut(s) 35, 301, 356, 393, 504, 864, 1114
BstMBI GATC 7 cut(s) 32, 298, 353, 390, 501, 861, 1111
BstMWI GCNNNNNNNGC 3 cut(s) 19, 168, 781
BstNI CCWGG 1 cut(s) 1043
BstSCI CCNGG 2 cut(s) 70, 1041
BstSFI CTRYAG 3 cut(s) 213, 593, 654
BstSLI GKGCMC 1 cut(s) 757
BstV1I GCAGC 5 cut(s) 72, 118, 795, 893, 1028
BstX2I RGATCY 1 cut(s) 353
BstYI RGATCY 1 cut(s) 353
BstZ17I GTATAC 1 cut(s) 763
BsuRI GGCC 1 cut(s) 70
BtrI CACGTC 1 cut(s) 838
BtsCI GGATG 4 cut(s) 162, 543, 644, 1003
BtsI GCAGTG 2 cut(s) 498, 803
BtsIMutI CAGTG 3 cut(s) 498, 511, 803
BveI ACCTGC 2 cut(s) 210, 400
Cac8I GCNNGC 2 cut(s) 11, 777
Cfr10I RCCGGY 2 cut(s) 18, 329
Cfr13I GGNCC 2 cut(s) 69, 935
Csp6I GTAC 5 cut(s) 124, 176, 418, 915, 982
CspAI ACCGGT 1 cut(s) 329
CspCI CAANNNNNGTGG 4 cut(s) 98, 133, 556, 591
CviAII CATG 5 cut(s) 64, 206, 262, 719, 859
CviQI GTAC 5 cut(s) 124, 176, 418, 915, 982
DpnI GATC 7 cut(s) 34, 300, 355, 392, 503, 863, 1113
DpnII GATC 7 cut(s) 32, 298, 353, 390, 501, 861, 1111
DriI GACNNNNNGTC 1 cut(s) 185
Eam1105I GACNNNNNGTC 1 cut(s) 185
Eco130I CCWWGG 2 cut(s) 445, 812
Eco47I GGWCC 1 cut(s) 935
Eco88I CYCGRG 1 cut(s) 1061
EcoNI CCTNNNNNAGG 1 cut(s) 672
EcoO109I RGGNCCY 1 cut(s) 935
EcoRII CCWGG 1 cut(s) 1041
EcoT14I CCWWGG 2 cut(s) 445, 812
ErhI CCWWGG 2 cut(s) 445, 812
FaeI CATG 5 cut(s) 67, 209, 265, 722, 862
FaqI GGGAC 1 cut(s) 526
FatI CATG 5 cut(s) 63, 205, 261, 718, 858
FbaI TGATCA 3 cut(s) 501, 861, 1111
FblI GTMKAC 1 cut(s) 762
Fnu4HI GCNGC 5 cut(s) 61, 107, 809, 907, 1017
FokI GGATG 4 cut(s) 169, 530, 651, 990
Fsp4HI GCNGC 5 cut(s) 61, 107, 809, 907, 1017
FspBI CTAG 3 cut(s) 14, 30, 200
GluI GCNGC 5 cut(s) 61, 107, 809, 907, 1017
GsaI CCCAGC 2 cut(s) 513, 663
GsuI CTGGAG 1 cut(s) 1064
HaeIII GGCC 1 cut(s) 70
HapII CCGG 4 cut(s) 19, 72, 330, 357
Hin1II CATG 5 cut(s) 67, 209, 265, 722, 862
HincII GTYRAC 1 cut(s) 954
HindII GTYRAC 1 cut(s) 954
HindIII AAGCTT 2 cut(s) 632, 773
HinfI GANTC 4 cut(s) 26, 82, 360, 441
HpaI GTTAAC 1 cut(s) 954
HpaII CCGG 4 cut(s) 19, 72, 330, 357
HphI GGTGA 1 cut(s) 319
Hpy166II GTNNAC 5 cut(s) 176, 716, 755, 763, 954
Hpy188I TCNGA 3 cut(s) 303, 313, 1116
Hpy188III TCNNGA 8 cut(s) 30, 245, 296, 357, 424, 890, 1061, 1070
Hpy8I GTNNAC 5 cut(s) 176, 716, 755, 763, 954
Hpy99I CGWCG 1 cut(s) 368
HpyAV CCTTC 2 cut(s) 549, 949
HpyCH4III ACNGT 2 cut(s) 283, 700
HpyCH4IV ACGT 1 cut(s) 837
HpyCH4V TGCA 7 cut(s) 491, 595, 607, 642, 755, 779, 833
HpyF10VI GCNNNNNNNGC 3 cut(s) 19, 168, 781
HpySE526I ACGT 1 cut(s) 837
Hsp92II CATG 5 cut(s) 67, 209, 265, 722, 862
Kpn2I TCCGGA 1 cut(s) 356
Ksp22I TGATCA 3 cut(s) 501, 861, 1111
KspAI GTTAAC 1 cut(s) 954
Kzo9I GATC 7 cut(s) 32, 298, 353, 390, 501, 861, 1111
LmnI GCTCC 1 cut(s) 816
Lsp1109I GCAGC 5 cut(s) 72, 118, 795, 893, 1028
LweI GCATC 2 cut(s) 186, 629
MaeI CTAG 3 cut(s) 14, 30, 200
MaeII ACGT 1 cut(s) 837
MaeIII GTNAC 3 cut(s) 208, 325, 1099
MalI GATC 7 cut(s) 34, 300, 355, 392, 503, 863, 1113
MboI GATC 7 cut(s) 32, 298, 353, 390, 501, 861, 1111
MboII GAAGA 4 cut(s) 657, 738, 752, 1060
MflI RGATCY 1 cut(s) 353
MhlI GDGCHC 1 cut(s) 757
MluCI AATT 8 cut(s) 236, 480, 532, 704, 745, 822, 852, 946
MlyI GAGTC 2 cut(s) 91, 450
MmeI TCCRAC 1 cut(s) 59
MroI TCCGGA 1 cut(s) 356
MroXI GAANNNNTTC 1 cut(s) 282
MseI TTAA 6 cut(s) 42, 266, 479, 531, 851, 953
MspI CCGG 4 cut(s) 19, 72, 330, 357
MspR9I CCNGG 2 cut(s) 72, 1043
MvaI CCWGG 1 cut(s) 1043
MwoI GCNNNNNNNGC 3 cut(s) 19, 168, 781
NciI CCSGG 1 cut(s) 72
NdeII GATC 7 cut(s) 32, 298, 353, 390, 501, 861, 1111
NlaIII CATG 5 cut(s) 67, 209, 265, 722, 862
NlaIV GGNNCC 3 cut(s) 243, 355, 937
NmuCI GTSAC 1 cut(s) 325
PdmI GAANNNNTTC 1 cut(s) 282
PfeI GAWTC 2 cut(s) 26, 360
PinAI ACCGGT 1 cut(s) 329
PkrI GCNGC 5 cut(s) 62, 108, 810, 908, 1018
PleI GAGTC 2 cut(s) 90, 449
PpsI GAGTC 2 cut(s) 90, 449
PpuMI RGGWCCY 1 cut(s) 935
PsiI TTATAA 1 cut(s) 630
Psp5II RGGWCCY 1 cut(s) 935
Psp6I CCWGG 1 cut(s) 1041
PspFI CCCAGC 2 cut(s) 509, 659
PspGI CCWGG 1 cut(s) 1041
PspN4I GGNNCC 3 cut(s) 243, 355, 937
PspPI GGNCC 2 cut(s) 69, 935
PspPPI RGGWCCY 1 cut(s) 935
PstI CTGCAG 1 cut(s) 597
PsuI RGATCY 1 cut(s) 353
RsaI GTAC 5 cut(s) 125, 177, 419, 916, 983
RsaNI GTAC 5 cut(s) 124, 176, 418, 915, 982
SaqAI TTAA 6 cut(s) 42, 266, 479, 531, 851, 953
SatI GCNGC 5 cut(s) 61, 107, 809, 907, 1017
Sau3AI GATC 7 cut(s) 32, 298, 353, 390, 501, 861, 1111
Sau96I GGNCC 2 cut(s) 69, 935
ScaI AGTACT 2 cut(s) 419, 983
SchI GAGTC 2 cut(s) 91, 450
ScrFI CCNGG 2 cut(s) 72, 1043
SduI GDGCHC 1 cut(s) 757
SfaNI GCATC 2 cut(s) 186, 629
SfcI CTRYAG 3 cut(s) 213, 593, 654
SgrAI CRCCGGYG 1 cut(s) 329
SinI GGWCC 1 cut(s) 935
SmlI CTYRAG 1 cut(s) 94
SmoI CTYRAG 1 cut(s) 94
Sse9I AATT 8 cut(s) 236, 480, 532, 704, 745, 822, 852, 946
SsiI CCGC 1 cut(s) 75
SspI AATATT 1 cut(s) 571
SspMI CTAG 3 cut(s) 14, 30, 200
StyD4I CCNGG 2 cut(s) 70, 1041
StyI CCWWGG 2 cut(s) 445, 812
TaaI ACNGT 2 cut(s) 283, 700
TaiI ACGT 1 cut(s) 840
TaqI TCGA 3 cut(s) 85, 146, 363
TasI AATT 8 cut(s) 236, 480, 532, 704, 745, 822, 852, 946
TatI WGTACW 2 cut(s) 417, 981
TfiI GAWTC 2 cut(s) 26, 360
Tru1I TTAA 6 cut(s) 42, 266, 479, 531, 851, 953
Tru9I TTAA 6 cut(s) 42, 266, 479, 531, 851, 953
TscAI CASTG 3 cut(s) 498, 511, 810
TseFI GTSAC 1 cut(s) 325
TseI GCWGC 5 cut(s) 60, 106, 808, 906, 1016
Tsp45I GTSAC 1 cut(s) 325
TspDTI ATGAA 1 cut(s) 604
TspGWI ACGGA 1 cut(s) 169
TspRI CASTG 3 cut(s) 498, 511, 810
VneI GTGCAC 1 cut(s) 753
VpaK11BI GGWCC 1 cut(s) 935
XagI CCTNNNNNAGG 1 cut(s) 672
XapI RAATTY 2 cut(s) 704, 745
XbaI TCTAGA 1 cut(s) 29
XmiI GTMKAC 1 cut(s) 762
XmnI GAANNNNTTC 1 cut(s) 282
XspI CTAG 3 cut(s) 14, 30, 200
ZrmI AGTACT 2 cut(s) 419, 983
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.