RLG00000036240

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0001786
Physical Location & Seq
Reverse (-)
77934213 .. 77935870
1658 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036240

Sequence Viewer

Length: 999 bp
ATGGACACAGGCAACAACAATTACCTCACAACATTGTTTAAGGGTAACCATTATCCATATGGCCAAGATTTTCCAGGTCATGTTACTACAGGCAGGTTTTCTAACGGAAAATTGGTTCCCGACTTCCTTGCCTCTATGTTAAACATCAAAGAAACTGTTCCTCCTTTTCTTGATCCGAGCCTCTCGGATAACAACCTTGTCACCGGTGTCAGCTTTGCTTCGGGTGGATCCGGATTCGACGATATTACAGGTAGTGTAGCTGGGATCATTCCATTTTCAAAGCAGGTTGAGTACTTCAAAAGATACATAGTGAGAGTCCAAGGTATTGTAGGGGAAAAGGAGGCAAAGAAGTTAATTAGTAGTGCAGTGGTTGTGATCAGTGCTGGGACTAATGACTTTGGTTTTAATTTCTATGACATCCCCACAAGAAGGTTAGAGTTCAATATTAGTGGCTACCAAGATTTTCTGCAGAATAAACTGCATATATTCATAGAGGAACTCTATGAGCTTGGATGCAGAAGAATGGCTATAGCTGGGCTTCCTCCAGTAGGTTGCCTACCCATACAAATAACTGTAAAATTTGAAAGTCCACATGATAGAAGATGTATAGAGGAAGAAAATTTGGATGCACTTGCATACAATCAGAAGCTTGCAAGGCTCTTGCCCAAAATACAATCACTGCTGCCAGGGAGCAAAATTATGTATGCAGACGTGTATATGCCTTTAATTGACATGATCAACAATCCACTAAACTATGGTTTTCTGGAAACAAAAAGAGGCTGCTGTGGTACTGGGTTAGTGGAAGCAGGACCCTTCTGTAATTCATTAACAGCAGTATGTGATAATGTCTTGGAGTACTTGTTTTGGGATAGCATACATCCAAGTCAAGCAGCATATCTCTACATTTCAAAATACCTGGAGAAGAAAATCATTCCCGAGCTTCTTGATTACGATAATCAATCACAGCCGTTAGTTACTCTATTTGATCAGACAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

333

Amino Acids

37.05

Weight (kDa)

5.21

Isoelectric Point (pI)

43.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 27 - 305 3.9e-29 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 84, 274
AccIII TCCGGA 1 cut(s) 230
AclWI GGATC 4 cut(s) 167, 222, 235, 272
AcoI YGGCCR 1 cut(s) 61
AcsI RAATTY 2 cut(s) 578, 619
AfaI GTAC 3 cut(s) 293, 790, 857
AfiI CCNNNNNNNGG 2 cut(s) 429, 548
AflIII ACRYGT 1 cut(s) 711
AgeI ACCGGT 1 cut(s) 203
AgsI TTSAA 5 cut(s) 279, 298, 442, 584, 909
AjiI CACGTC 1 cut(s) 712
AjnI CCWGG 3 cut(s) 73, 685, 915
AloI GAACNNNNNNTCC 2 cut(s) 99, 131
AluBI AGCT 6 cut(s) 213, 260, 508, 533, 649, 940
AluI AGCT 6 cut(s) 213, 260, 508, 533, 649, 940
AlwI GGATC 4 cut(s) 167, 222, 235, 272
Ama87I CYCGRG 1 cut(s) 935
Aor13HI TCCGGA 1 cut(s) 230
AoxI GGCC 1 cut(s) 61
ApeKI GCWGC 3 cut(s) 682, 780, 890
ApoI RAATTY 2 cut(s) 578, 619
AsiGI ACCGGT 1 cut(s) 203
Asp700I GAANNNNTTC 1 cut(s) 156
AspS9I GGNCC 1 cut(s) 809
AsuHPI GGTGA 1 cut(s) 193
AvaI CYCGRG 1 cut(s) 935
AvaII GGWCC 1 cut(s) 809
BalI TGGCCA 1 cut(s) 63
BamHI GGATCC 1 cut(s) 227
BbvI GCAGC 3 cut(s) 669, 767, 902
BceAI ACGGC 1 cut(s) 952
BcgI CGANNNNNNTGC 2 cut(s) 110, 144
BciT130I CCWGG 3 cut(s) 75, 687, 917
BclI TGATCA 3 cut(s) 375, 735, 985
BfmI CTRYAG 3 cut(s) 87, 467, 528
BfuAI ACCTGC 2 cut(s) 84, 274
BisI GCNGC 3 cut(s) 683, 781, 891
BlsI GCNGC 3 cut(s) 684, 782, 892
BmcAI AGTACT 2 cut(s) 293, 857
Bme1390I CCNGG 3 cut(s) 75, 687, 917
Bme18I GGWCC 1 cut(s) 809
BmeT110I CYCGRG 1 cut(s) 935
BmgBI CACGTC 1 cut(s) 712
BmgT120I GGNCC 1 cut(s) 809
BmiI GGNNCC 3 cut(s) 117, 229, 811
BmrFI CCNGG 3 cut(s) 75, 687, 917
BmrI ACTGGG 1 cut(s) 801
BmsI GCATC 2 cut(s) 503, 616
BmuI ACTGGG 1 cut(s) 801
BpmI CTGGAG 2 cut(s) 528, 938
BsaJI CCNNGG 2 cut(s) 319, 686
BsaWI WCCGGW 2 cut(s) 203, 230
BsaXI ACNNNNNCTCC 4 cut(s) 145, 175, 845, 875
Bsc4I CCNNNNNNNGG 2 cut(s) 429, 548
Bse118I RCCGGY 1 cut(s) 203
Bse1I ACTGG 2 cut(s) 545, 796
BseAI TCCGGA 1 cut(s) 230
BseBI CCWGG 3 cut(s) 75, 687, 917
BseDI CCNNGG 2 cut(s) 319, 686
BseGI GGATG 4 cut(s) 417, 518, 631, 877
BseLI CCNNNNNNNGG 2 cut(s) 429, 548
BseNI ACTGG 2 cut(s) 545, 796
BseXI GCAGC 3 cut(s) 669, 767, 902
BseYI CCCAGC 3 cut(s) 260, 383, 533
BsgI GTGCAG 1 cut(s) 384
BshFI GGCC 1 cut(s) 63
BshTI ACCGGT 1 cut(s) 203
BsiHKCI CYCGRG 1 cut(s) 935
BsiSI CCGG 2 cut(s) 204, 231
BslFI GGGAC 1 cut(s) 400
BslI CCNNNNNNNGG 2 cut(s) 429, 548
BsmFI GGGAC 1 cut(s) 400
BsnI GGCC 1 cut(s) 63
BsoBI CYCGRG 1 cut(s) 935
Bsp13I TCCGGA 1 cut(s) 230
Bsp143I GATC 6 cut(s) 172, 227, 264, 375, 735, 985
BspANI GGCC 1 cut(s) 63
BspEI TCCGGA 1 cut(s) 230
BspLI GGNNCC 3 cut(s) 117, 229, 811
BspMAI CTGCAG 1 cut(s) 471
BspMI ACCTGC 2 cut(s) 84, 274
BspPI GGATC 4 cut(s) 167, 222, 235, 272
BsrFI RCCGGY 1 cut(s) 203
BsrI ACTGG 2 cut(s) 545, 796
BssAI RCCGGY 1 cut(s) 203
BssECI CCNNGG 2 cut(s) 319, 686
BssMI GATC 6 cut(s) 172, 227, 264, 375, 735, 985
BssT1I CCWWGG 1 cut(s) 319
Bst2UI CCWGG 3 cut(s) 75, 687, 917
Bst4CI ACNGT 2 cut(s) 157, 574
BstC8I GCNNGC 1 cut(s) 651
BstEII GGTNACC 1 cut(s) 44
BstENI CCTNNNNNAGG 1 cut(s) 546
BstF5I GGATG 4 cut(s) 417, 518, 631, 877
BstKTI GATC 6 cut(s) 175, 230, 267, 378, 738, 988
BstMBI GATC 6 cut(s) 172, 227, 264, 375, 735, 985
BstMWI GCNNNNNNNGC 1 cut(s) 655
BstNI CCWGG 3 cut(s) 75, 687, 917
BstPI GGTNACC 1 cut(s) 44
BstSCI CCNGG 3 cut(s) 73, 685, 915
BstSFI CTRYAG 3 cut(s) 87, 467, 528
BstV1I GCAGC 3 cut(s) 669, 767, 902
BstX2I RGATCY 1 cut(s) 227
BstYI RGATCY 1 cut(s) 227
BsuRI GGCC 1 cut(s) 63
BtrI CACGTC 1 cut(s) 712
BtsCI GGATG 4 cut(s) 417, 518, 631, 877
BtsI GCAGTG 2 cut(s) 372, 677
BtsIMutI CAGTG 3 cut(s) 372, 385, 677
BveI ACCTGC 2 cut(s) 84, 274
Cac8I GCNNGC 1 cut(s) 651
Cfr10I RCCGGY 1 cut(s) 203
Cfr13I GGNCC 1 cut(s) 809
Csp6I GTAC 3 cut(s) 292, 789, 856
CspAI ACCGGT 1 cut(s) 203
CspCI CAANNNNNGTGG 2 cut(s) 430, 465
CviAII CATG 3 cut(s) 80, 593, 733
CviQI GTAC 3 cut(s) 292, 789, 856
DpnI GATC 6 cut(s) 174, 229, 266, 377, 737, 987
DpnII GATC 6 cut(s) 172, 227, 264, 375, 735, 985
EaeI YGGCCR 1 cut(s) 61
Eco130I CCWWGG 1 cut(s) 319
Eco47I GGWCC 1 cut(s) 809
Eco88I CYCGRG 1 cut(s) 935
Eco91I GGTNACC 1 cut(s) 44
EcoNI CCTNNNNNAGG 1 cut(s) 546
EcoO109I RGGNCCY 1 cut(s) 809
EcoO65I GGTNACC 1 cut(s) 44
EcoRII CCWGG 3 cut(s) 73, 685, 915
EcoT14I CCWWGG 1 cut(s) 319
ErhI CCWWGG 1 cut(s) 319
FaeI CATG 3 cut(s) 83, 596, 736
FaqI GGGAC 1 cut(s) 400
FatI CATG 3 cut(s) 79, 592, 732
FauNDI CATATG 1 cut(s) 58
FbaI TGATCA 3 cut(s) 375, 735, 985
Fnu4HI GCNGC 3 cut(s) 683, 781, 891
FokI GGATG 4 cut(s) 404, 525, 638, 864
Fsp4HI GCNGC 3 cut(s) 683, 781, 891
GluI GCNGC 3 cut(s) 683, 781, 891
GsaI CCCAGC 3 cut(s) 264, 387, 537
GsuI CTGGAG 2 cut(s) 528, 938
HaeIII GGCC 1 cut(s) 63
HapII CCGG 2 cut(s) 204, 231
Hin1II CATG 3 cut(s) 83, 596, 736
HindIII AAGCTT 1 cut(s) 647
HinfI GANTC 2 cut(s) 234, 315
HpaII CCGG 2 cut(s) 204, 231
HphI GGTGA 1 cut(s) 193
Hpy166II GTNNAC 1 cut(s) 590
Hpy188I TCNGA 4 cut(s) 177, 187, 645, 990
Hpy188III TCNNGA 6 cut(s) 119, 170, 231, 764, 935, 944
Hpy8I GTNNAC 1 cut(s) 590
Hpy99I CGWCG 1 cut(s) 242
HpyAV CCTTC 2 cut(s) 423, 823
HpyCH4III ACNGT 2 cut(s) 157, 574
HpyCH4IV ACGT 1 cut(s) 711
HpyCH4V TGCA 8 cut(s) 365, 469, 481, 516, 629, 635, 653, 707
HpyF10VI GCNNNNNNNGC 1 cut(s) 655
HpySE526I ACGT 1 cut(s) 711
Hsp92II CATG 3 cut(s) 83, 596, 736
Kpn2I TCCGGA 1 cut(s) 230
Ksp22I TGATCA 3 cut(s) 375, 735, 985
Kzo9I GATC 6 cut(s) 172, 227, 264, 375, 735, 985
LmnI GCTCC 1 cut(s) 690
Lsp1109I GCAGC 3 cut(s) 669, 767, 902
LweI GCATC 2 cut(s) 503, 616
MaeII ACGT 1 cut(s) 711
MaeIII GTNAC 4 cut(s) 44, 82, 199, 973
MalI GATC 6 cut(s) 174, 229, 266, 377, 737, 987
MboI GATC 6 cut(s) 172, 227, 264, 375, 735, 985
MboII GAAGA 4 cut(s) 531, 612, 626, 934
MflI RGATCY 1 cut(s) 227
MlsI TGGCCA 1 cut(s) 63
MluCI AATT 9 cut(s) 19, 110, 354, 406, 578, 619, 696, 726, 820
MluNI TGGCCA 1 cut(s) 63
MlyI GAGTC 1 cut(s) 324
MnlI CCTC 9 cut(s) 35, 142, 171, 191, 334, 487, 552, 604, 770
Mox20I TGGCCA 1 cut(s) 63
MroI TCCGGA 1 cut(s) 230
MroXI GAANNNNTTC 1 cut(s) 156
MscI TGGCCA 1 cut(s) 63
MseI TTAA 6 cut(s) 39, 140, 353, 405, 725, 827
Msp20I TGGCCA 1 cut(s) 63
MspI CCGG 2 cut(s) 204, 231
MspR9I CCNGG 3 cut(s) 75, 687, 917
MvaI CCWGG 3 cut(s) 75, 687, 917
MwoI GCNNNNNNNGC 1 cut(s) 655
NdeI CATATG 1 cut(s) 58
NdeII GATC 6 cut(s) 172, 227, 264, 375, 735, 985
NlaIII CATG 3 cut(s) 83, 596, 736
NlaIV GGNNCC 3 cut(s) 117, 229, 811
NmuCI GTSAC 1 cut(s) 199
PdmI GAANNNNTTC 1 cut(s) 156
PfeI GAWTC 1 cut(s) 234
PinAI ACCGGT 1 cut(s) 203
PkrI GCNGC 3 cut(s) 684, 782, 892
PleI GAGTC 1 cut(s) 323
PpsI GAGTC 1 cut(s) 323
PpuMI RGGWCCY 1 cut(s) 809
Psp5II RGGWCCY 1 cut(s) 809
Psp6I CCWGG 3 cut(s) 73, 685, 915
PspEI GGTNACC 1 cut(s) 44
PspFI CCCAGC 3 cut(s) 260, 383, 533
PspGI CCWGG 3 cut(s) 73, 685, 915
PspN4I GGNNCC 3 cut(s) 117, 229, 811
PspPI GGNCC 1 cut(s) 809
PspPPI RGGWCCY 1 cut(s) 809
PstI CTGCAG 1 cut(s) 471
PsuI RGATCY 1 cut(s) 227
RsaI GTAC 3 cut(s) 293, 790, 857
RsaNI GTAC 3 cut(s) 292, 789, 856
SaqAI TTAA 6 cut(s) 39, 140, 353, 405, 725, 827
SatI GCNGC 3 cut(s) 683, 781, 891
Sau3AI GATC 6 cut(s) 172, 227, 264, 375, 735, 985
Sau96I GGNCC 1 cut(s) 809
ScaI AGTACT 2 cut(s) 293, 857
SchI GAGTC 1 cut(s) 324
ScrFI CCNGG 3 cut(s) 75, 687, 917
SfaNI GCATC 2 cut(s) 503, 616
SfcI CTRYAG 3 cut(s) 87, 467, 528
SgrAI CRCCGGYG 1 cut(s) 203
SinI GGWCC 1 cut(s) 809
Sse9I AATT 9 cut(s) 19, 110, 354, 406, 578, 619, 696, 726, 820
SspI AATATT 1 cut(s) 445
StyD4I CCNGG 3 cut(s) 73, 685, 915
StyI CCWWGG 1 cut(s) 319
TaaI ACNGT 2 cut(s) 157, 574
TaiI ACGT 1 cut(s) 714
TaqI TCGA 1 cut(s) 237
TasI AATT 9 cut(s) 19, 110, 354, 406, 578, 619, 696, 726, 820
TatI WGTACW 2 cut(s) 291, 855
TfiI GAWTC 1 cut(s) 234
Tru1I TTAA 6 cut(s) 39, 140, 353, 405, 725, 827
Tru9I TTAA 6 cut(s) 39, 140, 353, 405, 725, 827
TscAI CASTG 3 cut(s) 372, 385, 684
TseFI GTSAC 1 cut(s) 199
TseI GCWGC 3 cut(s) 682, 780, 890
Tsp45I GTSAC 1 cut(s) 199
TspDTI ATGAA 2 cut(s) 478, 813
TspGWI ACGGA 1 cut(s) 120
TspRI CASTG 3 cut(s) 372, 385, 684
VpaK11BI GGWCC 1 cut(s) 809
XagI CCTNNNNNAGG 1 cut(s) 546
XapI RAATTY 2 cut(s) 578, 619
XcmI CCANNNNNNNNNTGG 1 cut(s) 56
XmnI GAANNNNTTC 1 cut(s) 156
ZrmI AGTACT 2 cut(s) 293, 857
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.